AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82045


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82045.t1 MTR_2g055210 93.855 179 11 0 26 204 162 340 8.02e-121 346
MS.gene82045.t1 MTR_4g116300 34.197 193 103 5 26 203 66 249 1.28e-23 94.7
MS.gene82045.t1 MTR_4g094680 34.054 185 85 4 26 203 82 236 8.77e-23 92.0
MS.gene82045.t1 MTR_2g036170 35.393 178 86 7 29 203 100 251 5.42e-17 77.0
MS.gene82045.t1 MTR_4g078170 29.050 179 80 4 26 203 76 208 2.06e-15 72.0
MS.gene82045.t1 MTR_5g030130 29.609 179 81 4 26 203 96 230 6.67e-15 71.2
MS.gene82045.t1 MTR_5g007230 41.096 73 42 1 26 97 82 154 1.47e-12 64.3
MS.gene82045.t1 MTR_7g102790 41.667 72 41 1 26 96 71 142 2.49e-12 63.5
MS.gene82045.t1 MTR_8g066820 40.909 66 38 1 26 90 94 159 1.70e-11 61.6
MS.gene82045.t1 MTR_5g030130 40.580 69 40 1 26 93 96 164 2.70e-11 60.5
MS.gene82045.t1 MTR_1g062160 38.824 85 49 3 26 109 69 151 3.47e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82045.t1 AT3G60670 50.802 187 76 7 26 204 67 245 2.62e-44 148
MS.gene82045.t1 AT2G12646 35.385 195 104 5 26 203 63 252 6.98e-24 95.5
MS.gene82045.t1 AT2G12646 35.385 195 104 5 26 203 66 255 8.12e-24 95.5
MS.gene82045.t1 AT2G12646 35.385 195 104 5 26 203 66 255 8.12e-24 95.5
MS.gene82045.t1 AT1G31040 35.519 183 94 4 26 203 79 242 3.41e-22 90.9
MS.gene82045.t1 AT1G21000 30.508 177 111 5 29 203 77 243 6.10e-14 68.6
MS.gene82045.t1 AT1G21000 30.508 177 111 5 29 203 80 246 7.08e-14 68.6
MS.gene82045.t1 AT1G32700 42.254 71 40 1 26 95 34 104 2.55e-12 63.2
MS.gene82045.t1 AT1G32700 42.254 71 40 1 26 95 73 143 4.96e-12 62.8
MS.gene82045.t1 AT2G27930 40.000 75 35 1 26 90 69 143 6.40e-12 62.4
MS.gene82045.t1 AT2G27930 40.000 75 35 1 26 90 69 143 6.40e-12 62.4
MS.gene82045.t1 AT1G76590 30.508 177 108 8 29 203 82 245 1.65e-11 62.0
MS.gene82045.t1 AT2G27930 40.000 75 35 1 26 90 66 140 2.11e-11 60.5
MS.gene82045.t1 AT2G27930 40.000 75 35 1 26 90 66 140 2.11e-11 60.5
MS.gene82045.t1 AT4G17900 27.222 180 81 7 26 203 81 212 2.51e-11 60.8
MS.gene82045.t1 AT4G17900 26.667 180 82 6 26 203 95 226 2.66e-11 61.2
MS.gene82045.t1 AT2G27930 39.474 76 36 1 26 91 66 141 3.10e-11 60.5
MS.gene82045.t1 AT2G27930 39.474 76 36 1 26 91 66 141 3.10e-11 60.5

Find 52 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAACATTTGGTTCACTATC+TGG 0.237092 7.3:+59966837 MS.gene82045:CDS
GTAAGGGTCTTGAAGACTTC+TGG 0.303743 7.3:-59966247 None:intergenic
TGCCTCTCACAGACTCTTGC+AGG 0.308178 7.3:+59965766 MS.gene82045:CDS
GAGGAGCATCTCTTTGGAAT+TGG 0.338342 7.3:-59966201 None:intergenic
TTGCTTGAGGAGCATCTCTT+TGG 0.338603 7.3:-59966207 None:intergenic
AACCGAACTTCATCCGGTTC+CGG 0.371774 7.3:+59966957 MS.gene82045:CDS
AGTCACTTGGTGAAAACATT+TGG 0.391874 7.3:+59966825 MS.gene82045:CDS
GAGAGAGAACAGAATTTGTA+AGG 0.394736 7.3:-59966264 None:intergenic
CACCGGAACCGGATGAAGTT+CGG 0.397970 7.3:-59966959 None:intergenic
TCTCGGAGAGTCTGATGAAT+AGG 0.410323 7.3:+59967111 MS.gene82045:CDS
GGTTCAAACCGAACTTCATC+CGG 0.422747 7.3:+59966951 MS.gene82045:CDS
ATGCCACCATTATCTGATTC+TGG 0.428827 7.3:+59966882 MS.gene82045:CDS
GGGGATGTATCCACCGGAAC+CGG 0.447568 7.3:-59966970 None:intergenic
GCCTGCATGCTCGCCGGTGT+CGG 0.449198 7.3:+59967085 MS.gene82045:CDS
CGGTAGTAGCGGTGCAAGCA+AGG 0.456996 7.3:-59967010 None:intergenic
GGATGAAGTTCGGTTTGAAC+CGG 0.475769 7.3:-59966949 None:intergenic
TCTAGTCCAGAATCAGATAA+TGG 0.486066 7.3:-59966888 None:intergenic
CTCGCCGGTGTCGGAAATCT+CGG 0.497072 7.3:+59967094 MS.gene82045:CDS
GATGGTTCGAGAACCGAGTC+AGG 0.499983 7.3:-59966927 None:intergenic
GTTGTCCGAAAGAAAAGAAC+CGG 0.516356 7.3:+59967035 MS.gene82045:CDS
ATTCTGTTCTCTCTCTTGCA+AGG 0.523330 7.3:+59966272 MS.gene82045:CDS
GTGTCGGCCTGCATGCTCGC+CGG 0.526462 7.3:+59967079 MS.gene82045:CDS
CAATATAACGGGGCCCTATG+TGG 0.529393 7.3:-59967146 None:intergenic
GACTCGGTTCTCGAACCATC+CGG 0.529624 7.3:+59966930 MS.gene82045:CDS
GTCTTGAAGACTTCTGGCAC+AGG 0.531073 7.3:-59966241 None:intergenic
GAGGAAAGGAACGCCACATA+GGG 0.533397 7.3:+59967133 MS.gene82045:CDS
TCCGACACCGGCGAGCATGC+AGG 0.535651 7.3:-59967086 None:intergenic
CTACAGTTCTACAGAACAAA+CGG 0.540640 7.3:+59966150 MS.gene82045:intron
CGGTCTAATGACACCTGACT+CGG 0.553504 7.3:+59966914 MS.gene82045:CDS
CGAACTTCATCCGGTTCCGG+TGG 0.558699 7.3:+59966960 MS.gene82045:CDS
ATCAATGGGGATGTATCCAC+CGG 0.561626 7.3:-59966976 None:intergenic
TGCTTGCACCGCTACTACCG+AGG 0.570706 7.3:+59967013 MS.gene82045:CDS
CGGAGAGTCTGATGAATAGG+AGG 0.571731 7.3:+59967114 MS.gene82045:CDS
GGAGGAAAGGAACGCCACAT+AGG 0.574415 7.3:+59967132 MS.gene82045:CDS
GGTAGTAGCGGTGCAAGCAA+GGG 0.580460 7.3:-59967009 None:intergenic
AGTCTGATGAATAGGAGGAA+AGG 0.580615 7.3:+59967119 MS.gene82045:CDS
GAAGTTCGGTTTGAACCGGA+TGG 0.598179 7.3:-59966945 None:intergenic
GGTTCTGCAGATATTGCTTG+AGG 0.599864 7.3:-59966220 None:intergenic
ATGCAGGCCGACACGTTGGT+CGG 0.604022 7.3:-59967070 None:intergenic
AGAATCAGATAATGGTGGCA+TGG 0.607517 7.3:-59966880 None:intergenic
TCTGATTCTGGACTAGATGA+CGG 0.614207 7.3:+59966894 MS.gene82045:CDS
AGAGAGAACAGAATTTGTAA+GGG 0.616060 7.3:-59966263 None:intergenic
AGTAGCGGTGCAAGCAAGGG+CGG 0.618674 7.3:-59967006 None:intergenic
CTCTCCGAGATTTCCGACAC+CGG 0.623268 7.3:-59967098 None:intergenic
TCGGACAACCTCGGTAGTAG+CGG 0.624276 7.3:-59967021 None:intergenic
AAGAGTCTGTGAGAGGCATG+AGG 0.626300 7.3:-59965761 None:intergenic
TCACTTTCCGACCAACGTGT+CGG 0.667850 7.3:+59967063 MS.gene82045:CDS
AACCTGCAAGAGTCTGTGAG+AGG 0.669613 7.3:-59965768 None:intergenic
GAGCATGCAGGCCGACACGT+TGG 0.682817 7.3:-59967074 None:intergenic
AGTCCAGAATCAGATAATGG+TGG 0.683117 7.3:-59966885 None:intergenic
TGGTCGGAAAGTGATGACAC+CGG 0.742801 7.3:-59967054 None:intergenic
GCATGAGGAGAAACACAGTG+AGG 0.772523 7.3:-59965746 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATTAAACTATGTATAT+TGG + chr7.3:59965996-59966015 MS.gene82045:intron 10.0%
!!! CATTTTTTTTTGTTATTTTA+TGG + chr7.3:59966784-59966803 MS.gene82045:intron 10.0%
!! TGAAAAAAATGTTTATTGTT+TGG + chr7.3:59965703-59965722 MS.gene82045:CDS 15.0%
!!! GTTAATTTTTACAAGAATTT+AGG + chr7.3:59966055-59966074 MS.gene82045:intron 15.0%
!! AAAAAATATGACGATGAAAA+TGG - chr7.3:59966612-59966631 None:intergenic 20.0%
!! ATATTCAATTCGTGTAAAAT+TGG - chr7.3:59966506-59966525 None:intergenic 20.0%
!!! ATGTGTATCATGATATTTTA+AGG + chr7.3:59965886-59965905 MS.gene82045:intron 20.0%
!!! ATGTTGAATTTAGTTTTAGT+TGG + chr7.3:59966554-59966573 MS.gene82045:intron 20.0%
!!! TAATTGTTTGTCTGATTTTA+TGG - chr7.3:59966714-59966733 None:intergenic 20.0%
!!! TACAATGATTTTATCATGTT+TGG + chr7.3:59966372-59966391 MS.gene82045:intron 20.0%
!!! TATCATGATATTTTAAGGAT+GGG + chr7.3:59965891-59965910 MS.gene82045:intron 20.0%
!!! TTCAATTTTTAGTGTATGTT+TGG + chr7.3:59966331-59966350 MS.gene82045:intron 20.0%
! AGATTAAATATGAATGTGAG+AGG - chr7.3:59966307-59966326 None:intergenic 25.0%
!! CTATTCAAGTAAGTGATTTT+TGG + chr7.3:59965943-59965962 MS.gene82045:intron 25.0%
!! TACCACAATTAATTTTGTGT+TGG + chr7.3:59966110-59966129 MS.gene82045:intron 25.0%
!!! GTATCATGATATTTTAAGGA+TGG + chr7.3:59965890-59965909 MS.gene82045:intron 25.0%
ACAAGAAAGAGAAAAGAAGA+AGG - chr7.3:59965794-59965813 None:intergenic 30.0%
AGAGAGAACAGAATTTGTAA+GGG - chr7.3:59966266-59966285 None:intergenic 30.0%
CACCAACACAAAATTAATTG+TGG - chr7.3:59966115-59966134 None:intergenic 30.0%
! AAAACATTTGGTTCACTATC+TGG + chr7.3:59966837-59966856 MS.gene82045:CDS 30.0%
CTACAGTTCTACAGAACAAA+CGG + chr7.3:59966150-59966169 MS.gene82045:intron 35.0%
GAGAGAGAACAGAATTTGTA+AGG - chr7.3:59966267-59966286 None:intergenic 35.0%
TCTAGTCCAGAATCAGATAA+TGG - chr7.3:59966891-59966910 None:intergenic 35.0%
! AGTCACTTGGTGAAAACATT+TGG + chr7.3:59966825-59966844 MS.gene82045:CDS 35.0%
! TTTATGGTTGTGTTTTCCAC+AGG + chr7.3:59966800-59966819 MS.gene82045:intron 35.0%
AGTCTGATGAATAGGAGGAA+AGG + chr7.3:59967119-59967138 MS.gene82045:CDS 40.0%
ATGCCACCATTATCTGATTC+TGG + chr7.3:59966882-59966901 MS.gene82045:CDS 40.0%
ATTCTGTTCTCTCTCTTGCA+AGG + chr7.3:59966272-59966291 MS.gene82045:CDS 40.0%
GTTGTCCGAAAGAAAAGAAC+CGG + chr7.3:59967035-59967054 MS.gene82045:CDS 40.0%
! AGAATCAGATAATGGTGGCA+TGG - chr7.3:59966883-59966902 None:intergenic 40.0%
! AGTCCAGAATCAGATAATGG+TGG - chr7.3:59966888-59966907 None:intergenic 40.0%
! TCTGATTCTGGACTAGATGA+CGG + chr7.3:59966894-59966913 MS.gene82045:CDS 40.0%
! TCTTTTCTTTCGGACAACCT+CGG - chr7.3:59967033-59967052 None:intergenic 40.0%
!!! TGACACCGGTTCTTTTCTTT+CGG - chr7.3:59967043-59967062 None:intergenic 40.0%
ATCAATGGGGATGTATCCAC+CGG - chr7.3:59966979-59966998 None:intergenic 45.0%
GGTTCAAACCGAACTTCATC+CGG + chr7.3:59966951-59966970 MS.gene82045:CDS 45.0%
GGTTCTGCAGATATTGCTTG+AGG - chr7.3:59966223-59966242 None:intergenic 45.0%
GTAAGGGTCTTGAAGACTTC+TGG - chr7.3:59966250-59966269 None:intergenic 45.0%
TCTCGGAGAGTCTGATGAAT+AGG + chr7.3:59967111-59967130 MS.gene82045:CDS 45.0%
! AAGGGCGGTTTTGCAATCAA+TGG - chr7.3:59966994-59967013 None:intergenic 45.0%
! AGGGCGGTTTTGCAATCAAT+GGG - chr7.3:59966993-59967012 None:intergenic 45.0%
! GGATGAAGTTCGGTTTGAAC+CGG - chr7.3:59966952-59966971 None:intergenic 45.0%
! TTTTCCACAGGTGAGTCACT+TGG + chr7.3:59966812-59966831 MS.gene82045:intron 45.0%
!! GAGGAGCATCTCTTTGGAAT+TGG - chr7.3:59966204-59966223 None:intergenic 45.0%
!! TTGCTTGAGGAGCATCTCTT+TGG - chr7.3:59966210-59966229 None:intergenic 45.0%
AACCGAACTTCATCCGGTTC+CGG + chr7.3:59966957-59966976 MS.gene82045:CDS 50.0%
AACCTGCAAGAGTCTGTGAG+AGG - chr7.3:59965771-59965790 None:intergenic 50.0%
CGGAGAGTCTGATGAATAGG+AGG + chr7.3:59967114-59967133 MS.gene82045:CDS 50.0%
CGGTCTAATGACACCTGACT+CGG + chr7.3:59966914-59966933 MS.gene82045:CDS 50.0%
GAGGAAAGGAACGCCACATA+GGG + chr7.3:59967133-59967152 MS.gene82045:CDS 50.0%
GCATGAGGAGAAACACAGTG+AGG - chr7.3:59965749-59965768 None:intergenic 50.0%
GTCTTGAAGACTTCTGGCAC+AGG - chr7.3:59966244-59966263 None:intergenic 50.0%
TCACTTTCCGACCAACGTGT+CGG + chr7.3:59967063-59967082 MS.gene82045:CDS 50.0%
TGGTCGGAAAGTGATGACAC+CGG - chr7.3:59967057-59967076 None:intergenic 50.0%
TTCACCAAGTGACTCACCTG+TGG - chr7.3:59966819-59966838 None:intergenic 50.0%
! AAGAGTCTGTGAGAGGCATG+AGG - chr7.3:59965764-59965783 None:intergenic 50.0%
! CAATATAACGGGGCCCTATG+TGG - chr7.3:59967149-59967168 None:intergenic 50.0%
! GAAGTTCGGTTTGAACCGGA+TGG - chr7.3:59966948-59966967 None:intergenic 50.0%
! GGGCGGTTTTGCAATCAATG+GGG - chr7.3:59966992-59967011 None:intergenic 50.0%
CACCGGAACCGGATGAAGTT+CGG - chr7.3:59966962-59966981 None:intergenic 55.0%
CTCTCCGAGATTTCCGACAC+CGG - chr7.3:59967101-59967120 None:intergenic 55.0%
GATGGTTCGAGAACCGAGTC+AGG - chr7.3:59966930-59966949 None:intergenic 55.0%
GGAGGAAAGGAACGCCACAT+AGG + chr7.3:59967132-59967151 MS.gene82045:CDS 55.0%
GGTAGTAGCGGTGCAAGCAA+GGG - chr7.3:59967012-59967031 None:intergenic 55.0%
TCGGACAACCTCGGTAGTAG+CGG - chr7.3:59967024-59967043 None:intergenic 55.0%
TGCCTCTCACAGACTCTTGC+AGG + chr7.3:59965766-59965785 MS.gene82045:CDS 55.0%
! GACTCGGTTCTCGAACCATC+CGG + chr7.3:59966930-59966949 MS.gene82045:CDS 55.0%
AGTAGCGGTGCAAGCAAGGG+CGG - chr7.3:59967009-59967028 None:intergenic 60.0%
ATGCAGGCCGACACGTTGGT+CGG - chr7.3:59967073-59967092 None:intergenic 60.0%
CGAACTTCATCCGGTTCCGG+TGG + chr7.3:59966960-59966979 MS.gene82045:CDS 60.0%
CGGTAGTAGCGGTGCAAGCA+AGG - chr7.3:59967013-59967032 None:intergenic 60.0%
GGGGATGTATCCACCGGAAC+CGG - chr7.3:59966973-59966992 None:intergenic 60.0%
TGCTTGCACCGCTACTACCG+AGG + chr7.3:59967013-59967032 MS.gene82045:CDS 60.0%
! CTCGCCGGTGTCGGAAATCT+CGG + chr7.3:59967094-59967113 MS.gene82045:CDS 60.0%
GAGCATGCAGGCCGACACGT+TGG - chr7.3:59967077-59967096 None:intergenic 65.0%
GCCTGCATGCTCGCCGGTGT+CGG + chr7.3:59967085-59967104 MS.gene82045:CDS 70.0%
TCCGACACCGGCGAGCATGC+AGG - chr7.3:59967089-59967108 None:intergenic 70.0%
! GTGTCGGCCTGCATGCTCGC+CGG + chr7.3:59967079-59967098 MS.gene82045:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 59965702 59967169 59965702 ID=MS.gene82045
chr7.3 mRNA 59965702 59967169 59965702 ID=MS.gene82045.t1;Parent=MS.gene82045
chr7.3 exon 59965702 59965787 59965702 ID=MS.gene82045.t1.exon1;Parent=MS.gene82045.t1
chr7.3 CDS 59965702 59965787 59965702 ID=cds.MS.gene82045.t1;Parent=MS.gene82045.t1
chr7.3 exon 59965908 59965950 59965908 ID=MS.gene82045.t1.exon2;Parent=MS.gene82045.t1
chr7.3 CDS 59965908 59965950 59965908 ID=cds.MS.gene82045.t1;Parent=MS.gene82045.t1
chr7.3 exon 59966156 59966293 59966156 ID=MS.gene82045.t1.exon3;Parent=MS.gene82045.t1
chr7.3 CDS 59966156 59966293 59966156 ID=cds.MS.gene82045.t1;Parent=MS.gene82045.t1
chr7.3 exon 59966822 59967169 59966822 ID=MS.gene82045.t1.exon4;Parent=MS.gene82045.t1
chr7.3 CDS 59966822 59967169 59966822 ID=cds.MS.gene82045.t1;Parent=MS.gene82045.t1
Gene Sequence

>MS.gene82045

ATGAAAAAAATGTTTATTGTTTGGATTGTTGCATCAGTCTTTGTCCTCACTGTGTTTCTCCTCATGCCTCTCACAGACTCTTGCAGGTTCCTTCTTCTTTTCTCTTTCTTGTTTTTAATAAATTAATTAATTAATTGAATTAATTTTAAGAAAAAAAAATGTTAATTTTTTCAGATAAGAAGATATGTGTATCATGATATTTTAAGGATGGGTGATGCTGCAAAGTTAATTGACTGTTCTTCTATTCAAGTAAGTGATTTTTGGTTTTTTGAGTTTATCATTTTTTATTAAAATATATATTAAACTATGTATATTGGTGTAATTTTCTTGTCAATTTTGATTTTAATTCTGAAGTTAATTTTTACAAGAATTTAGGTTATTTTTTTGTTGAGTTTATGTGTTTGTTATTACCACAATTAATTTTGTGTTGGTGTGTATGATGATGATGCTACAGTTCTACAGAACAAACGGTGCAAAAGTGATTTTTATAAATCAAAGACCAATTCCAAAGAGATGCTCCTCAAGCAATATCTGCAGAACCTGTGCCAGAAGTCTTCAAGACCCTTACAAATTCTGTTCTCTCTCTTGCAAGGTTCAGTTTTCCTCTCACATTCATATTTAATCTTCTTTTCAATTTTTAGTGTATGTTTGGTTACATATTTAAAGAAGTTACAATGATTTTATCATGTTTGGAATTTTTAAGTATAATTAGTTTTCTCTTTGAAGTTAATTTTAACTTAAGCTAGAATGTTATAAGTAGTTTTTTGCTTCTACAATTAATTCTTTTGTATTTATTTTCAACCAATTTTACACGAATTGAATATATACAAACATAAATTATAGTATTATTTTATGTTGAATTTAGTTTTAGTTGGAATCAATTTTATGTAATCATTTTATTCAAAATCCATTTTCATCGTCATATTTTTTTTAATCAAACAAACAATTACTTTAGTTAATTCTTTTTAATTTTATTTTTATTATTATTCTCATTTTTTCATTTTCATCACCATAAAATCAGACAAACAATTAATTGTTTTAATTCTTTTTATTTATTAAGTTTTAAATTTTATTATTATTCTCATTTTTTTTTGTTATTTTATGGTTGTGTTTTCCACAGGTGAGTCACTTGGTGAAAACATTTGGTTCACTATCTGGTTACTTACTCGAGTGCAACACCATGCCACCATTATCTGATTCTGGACTAGATGACGGTCTAATGACACCTGACTCGGTTCTCGAACCATCCGGTTCAAACCGAACTTCATCCGGTTCCGGTGGATACATCCCCATTGATTGCAAAACCGCCCTTGCTTGCACCGCTACTACCGAGGTTGTCCGAAAGAAAAGAACCGGTGTCATCACTTTCCGACCAACGTGTCGGCCTGCATGCTCGCCGGTGTCGGAAATCTCGGAGAGTCTGATGAATAGGAGGAAAGGAACGCCACATAGGGCCCCGTTATATTGA

Protein sequence

>MS.gene82045.t1

MKKMFIVWIVASVFVLTVFLLMPLTDSCRMGDAAKLIDCSSIQFYRTNGAKVIFINQRPIPKRCSSSNICRTCARSLQDPYKFCSLSCKVSHLVKTFGSLSGYLLECNTMPPLSDSGLDDGLMTPDSVLEPSGSNRTSSGSGGYIPIDCKTALACTATTEVVRKKRTGVITFRPTCRPACSPVSEISESLMNRRKGTPHRAPLY