Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82203.t1 | XP_003605334.1 | 94.3 | 159 | 9 | 0 | 1 | 159 | 1 | 159 | 4.50E-76 | 293.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82203.t1 | Q9FJK3 | 44.2 | 156 | 86 | 1 | 1 | 155 | 1 | 156 | 2.1e-26 | 120.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82203.t1 | G7JJI5 | 94.3 | 159 | 9 | 0 | 1 | 159 | 1 | 159 | 3.2e-76 | 293.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene82203.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82203.t1 | MTR_4g028720 | 94.340 | 159 | 9 | 0 | 1 | 159 | 1 | 159 | 1.59e-100 | 286 |
| MS.gene82203.t1 | MTR_7g011950 | 88.679 | 159 | 18 | 0 | 1 | 159 | 1 | 159 | 5.52e-96 | 274 |
| MS.gene82203.t1 | MTR_1g077320 | 83.648 | 159 | 26 | 0 | 1 | 159 | 1 | 159 | 4.75e-89 | 256 |
| MS.gene82203.t1 | MTR_1g077390 | 70.701 | 157 | 46 | 0 | 1 | 157 | 1 | 157 | 1.52e-77 | 228 |
| MS.gene82203.t1 | MTR_5g047580 | 69.231 | 156 | 48 | 0 | 1 | 156 | 1 | 156 | 7.50e-74 | 218 |
| MS.gene82203.t1 | MTR_1g084950 | 73.718 | 156 | 41 | 0 | 1 | 156 | 1 | 156 | 1.58e-72 | 215 |
| MS.gene82203.t1 | MTR_1g077300 | 72.956 | 159 | 41 | 1 | 1 | 159 | 1 | 157 | 2.54e-70 | 209 |
| MS.gene82203.t1 | MTR_1g090697 | 55.484 | 155 | 69 | 0 | 1 | 155 | 1 | 155 | 3.91e-56 | 173 |
| MS.gene82203.t1 | MTR_5g047560 | 57.692 | 156 | 50 | 1 | 1 | 156 | 1 | 140 | 4.41e-55 | 170 |
| MS.gene82203.t1 | MTR_1g090710 | 53.205 | 156 | 72 | 1 | 1 | 155 | 1 | 156 | 1.19e-53 | 167 |
| MS.gene82203.t1 | MTR_1g090783 | 51.634 | 153 | 74 | 0 | 3 | 155 | 2 | 154 | 5.59e-50 | 157 |
| MS.gene82203.t1 | MTR_4g032620 | 40.000 | 155 | 91 | 2 | 1 | 153 | 1 | 155 | 5.38e-39 | 132 |
| MS.gene82203.t1 | MTR_3g466980 | 43.791 | 153 | 85 | 1 | 1 | 152 | 1 | 153 | 1.15e-36 | 126 |
| MS.gene82203.t1 | MTR_3g065100 | 45.860 | 157 | 84 | 1 | 1 | 156 | 1 | 157 | 1.24e-35 | 124 |
| MS.gene82203.t1 | MTR_5g075380 | 36.538 | 156 | 98 | 1 | 1 | 155 | 1 | 156 | 9.29e-33 | 114 |
| MS.gene82203.t1 | MTR_4g063790 | 38.994 | 159 | 95 | 2 | 1 | 157 | 1 | 159 | 3.92e-32 | 113 |
| MS.gene82203.t1 | MTR_3g466830 | 40.127 | 157 | 93 | 1 | 3 | 158 | 2 | 158 | 4.93e-31 | 110 |
| MS.gene82203.t1 | MTR_8g036130 | 37.107 | 159 | 96 | 2 | 1 | 155 | 1 | 159 | 1.63e-29 | 108 |
| MS.gene82203.t1 | MTR_3g466890 | 38.961 | 154 | 93 | 1 | 3 | 155 | 2 | 155 | 1.74e-28 | 103 |
| MS.gene82203.t1 | MTR_4g031910 | 42.000 | 150 | 85 | 2 | 1 | 148 | 1 | 150 | 2.90e-28 | 104 |
| MS.gene82203.t1 | MTR_3g031240 | 35.220 | 159 | 101 | 2 | 1 | 157 | 1 | 159 | 1.31e-27 | 101 |
| MS.gene82203.t1 | MTR_3g467080 | 35.404 | 161 | 102 | 2 | 1 | 159 | 1 | 161 | 1.76e-27 | 100 |
| MS.gene82203.t1 | MTR_4g032260 | 38.750 | 160 | 96 | 2 | 1 | 158 | 1 | 160 | 2.56e-27 | 100 |
| MS.gene82203.t1 | MTR_3g031100 | 36.076 | 158 | 97 | 3 | 1 | 155 | 1 | 157 | 5.03e-26 | 99.4 |
| MS.gene82203.t1 | MTR_4g032290 | 38.509 | 161 | 97 | 2 | 1 | 159 | 1 | 161 | 1.18e-25 | 98.2 |
| MS.gene82203.t1 | MTR_2g016210 | 35.897 | 156 | 98 | 2 | 1 | 154 | 1 | 156 | 1.27e-24 | 95.5 |
| MS.gene82203.t1 | MTR_2g035610 | 39.831 | 118 | 70 | 1 | 1 | 117 | 1 | 118 | 2.66e-22 | 86.3 |
| MS.gene82203.t1 | MTR_2g035580 | 34.194 | 155 | 101 | 1 | 1 | 154 | 1 | 155 | 4.82e-21 | 84.3 |
| MS.gene82203.t1 | MTR_3g465410 | 35.878 | 131 | 82 | 2 | 29 | 157 | 1 | 131 | 1.01e-20 | 82.8 |
| MS.gene82203.t1 | MTR_3g466900 | 36.000 | 125 | 79 | 1 | 32 | 155 | 2 | 126 | 1.12e-17 | 74.7 |
| MS.gene82203.t1 | MTR_4g019670 | 32.653 | 147 | 95 | 2 | 3 | 148 | 6 | 149 | 2.66e-17 | 78.2 |
| MS.gene82203.t1 | MTR_3g466930 | 36.508 | 126 | 77 | 3 | 32 | 155 | 2 | 126 | 2.87e-17 | 73.6 |
| MS.gene82203.t1 | MTR_4g028800 | 44.444 | 72 | 38 | 1 | 1 | 72 | 1 | 70 | 7.91e-17 | 70.9 |
| MS.gene82203.t1 | MTR_7g106510 | 37.037 | 108 | 67 | 1 | 3 | 109 | 6 | 113 | 1.59e-16 | 75.9 |
| MS.gene82203.t1 | MTR_1g033370 | 41.489 | 94 | 54 | 1 | 64 | 156 | 28 | 121 | 6.61e-14 | 64.7 |
| MS.gene82203.t1 | MTR_7g055800 | 32.479 | 117 | 52 | 2 | 1 | 117 | 1 | 90 | 1.22e-13 | 63.2 |
| MS.gene82203.t1 | MTR_1g033930 | 39.796 | 98 | 58 | 1 | 62 | 158 | 20 | 117 | 1.79e-13 | 63.5 |
| MS.gene82203.t1 | MTR_1g033790 | 38.776 | 98 | 59 | 1 | 62 | 158 | 20 | 117 | 4.27e-12 | 60.1 |
| MS.gene82203.t1 | MTR_7g055940 | 35.577 | 104 | 40 | 3 | 1 | 104 | 1 | 77 | 5.98e-12 | 58.5 |
| MS.gene82203.t1 | MTR_6g005450 | 31.132 | 106 | 67 | 3 | 1 | 100 | 1 | 106 | 1.80e-11 | 61.2 |
| MS.gene82203.t1 | MTR_7g055790 | 37.615 | 109 | 51 | 2 | 1 | 109 | 59 | 150 | 2.19e-11 | 58.9 |
| MS.gene82203.t1 | MTR_3g093900 | 27.815 | 151 | 106 | 2 | 3 | 151 | 6 | 155 | 3.75e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82203.t1 | AT3G05860 | 38.926 | 149 | 90 | 1 | 1 | 148 | 1 | 149 | 1.55e-29 | 107 |
| MS.gene82203.t1 | AT5G48670 | 44.444 | 153 | 84 | 1 | 1 | 152 | 1 | 153 | 2.92e-29 | 109 |
| MS.gene82203.t1 | AT3G05860 | 38.926 | 149 | 90 | 1 | 1 | 148 | 1 | 149 | 7.29e-29 | 106 |
| MS.gene82203.t1 | AT3G05860 | 38.926 | 149 | 90 | 1 | 1 | 148 | 1 | 149 | 1.07e-28 | 106 |
| MS.gene82203.t1 | AT5G26630 | 38.750 | 160 | 97 | 1 | 1 | 159 | 1 | 160 | 4.39e-27 | 101 |
| MS.gene82203.t1 | AT1G65300 | 35.211 | 142 | 91 | 1 | 3 | 143 | 2 | 143 | 2.36e-24 | 95.9 |
| MS.gene82203.t1 | AT1G65330 | 36.054 | 147 | 93 | 1 | 3 | 148 | 2 | 148 | 4.41e-24 | 95.1 |
| MS.gene82203.t1 | AT5G27960 | 41.026 | 117 | 66 | 2 | 4 | 119 | 2 | 116 | 4.39e-22 | 90.5 |
| MS.gene82203.t1 | AT5G26650 | 41.026 | 117 | 66 | 2 | 4 | 119 | 2 | 116 | 5.53e-22 | 90.9 |
| MS.gene82203.t1 | AT2G28700 | 39.216 | 102 | 61 | 1 | 1 | 101 | 1 | 102 | 7.03e-20 | 84.7 |
| MS.gene82203.t1 | AT1G22590 | 32.479 | 117 | 78 | 1 | 1 | 117 | 1 | 116 | 3.46e-19 | 79.7 |
| MS.gene82203.t1 | AT1G31630 | 29.032 | 155 | 108 | 2 | 3 | 156 | 2 | 155 | 4.05e-19 | 82.4 |
| MS.gene82203.t1 | AT5G27810 | 40.625 | 96 | 56 | 1 | 29 | 123 | 1 | 96 | 7.55e-18 | 75.1 |
| MS.gene82203.t1 | AT5G26580 | 39.604 | 101 | 48 | 1 | 1 | 101 | 1 | 88 | 4.91e-17 | 77.0 |
| MS.gene82203.t1 | AT1G31640 | 37.069 | 116 | 71 | 2 | 3 | 117 | 2 | 116 | 2.00e-16 | 75.9 |
| MS.gene82203.t1 | AT2G40210 | 38.835 | 103 | 58 | 2 | 1 | 100 | 1 | 101 | 1.86e-13 | 67.0 |
| MS.gene82203.t1 | AT5G06500 | 29.375 | 160 | 107 | 3 | 1 | 157 | 1 | 157 | 1.88e-13 | 65.9 |
Find 25 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTTTCTTCCTCCGCTTGC+AGG | 0.240089 | 4.2:-75772475 | None:intergenic |
| TTTGCAAAAGGAAACTTTCT+TGG | 0.256030 | 4.2:-75772668 | None:intergenic |
| TGTTTAGCTTCCTTTAGATT+TGG | 0.322339 | 4.2:-75772591 | None:intergenic |
| AGCTGAACATTCGTATGATT+TGG | 0.323437 | 4.2:+75772748 | MS.gene82203:CDS |
| TCAGAAACCGTCACATCATC+TGG | 0.401819 | 4.2:-75772792 | None:intergenic |
| CTTAAATGATTCAACAAGAA+AGG | 0.417321 | 4.2:+75772449 | MS.gene82203:CDS |
| CAGAGCTCATATATCAAAGA+TGG | 0.456034 | 4.2:+75772630 | MS.gene82203:CDS |
| TGCAAAAGATTACTAAAGCT+AGG | 0.481563 | 4.2:+75772685 | MS.gene82203:CDS |
| GCAAAAGATTACTAAAGCTA+GGG | 0.487712 | 4.2:+75772686 | MS.gene82203:CDS |
| ACCTGCAAGCGGAGGAAGAA+AGG | 0.493077 | 4.2:+75772474 | MS.gene82203:CDS |
| CAAAGTCATGGCTAGGAAGA+AGG | 0.502352 | 4.2:+75772410 | None:intergenic |
| GGTTTCTGATTATAAAGACT+TGG | 0.508824 | 4.2:+75772806 | MS.gene82203:CDS |
| AGAGGTGTGGCCAAATCTAA+AGG | 0.522555 | 4.2:+75772581 | MS.gene82203:CDS |
| AGAGAACTCACTATTCTTTG+TGG | 0.526508 | 4.2:+75772513 | MS.gene82203:CDS |
| TCCTTTCGATTCTAAAACAG+AGG | 0.550758 | 4.2:+75772563 | MS.gene82203:CDS |
| TCGGAAATTATAGCACATGC+TGG | 0.559487 | 4.2:-75772540 | None:intergenic |
| GAAGGAAGTTGATGACAAGA+TGG | 0.579913 | 4.2:+75772851 | MS.gene82203:CDS |
| AACATTGCAAAGTCATGGCT+AGG | 0.583737 | 4.2:+75772403 | None:intergenic |
| AGAGCTCATATATCAAAGAT+GGG | 0.593695 | 4.2:+75772631 | MS.gene82203:CDS |
| GAAGAAAGGTATCATCAAGA+AGG | 0.595296 | 4.2:+75772488 | MS.gene82203:CDS |
| GAAAGGCGACCTGCAAGCGG+AGG | 0.605888 | 4.2:+75772466 | MS.gene82203:CDS |
| CAAGAAAGGCGACCTGCAAG+CGG | 0.616094 | 4.2:+75772463 | MS.gene82203:CDS |
| AAGCTAAACAGATGATTGAG+AGG | 0.630265 | 4.2:+75772604 | MS.gene82203:CDS |
| CAAGCTGCCAGATGATGTGA+CGG | 0.645186 | 4.2:+75772785 | MS.gene82203:CDS |
| TCGATTCTAAAACAGAGGTG+TGG | 0.664805 | 4.2:+75772568 | MS.gene82203:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTGATTGAAAAAAATATGA+AGG | + | chr4.2:75772833-75772852 | MS.gene82203:CDS | 20.0% |
| ! | CTTAAATGATTCAACAAGAA+AGG | + | chr4.2:75772449-75772468 | MS.gene82203:CDS | 25.0% |
| !! | CTTTAGTAATCTTTTGCAAA+AGG | - | chr4.2:75772683-75772702 | None:intergenic | 25.0% |
| AGAGCTCATATATCAAAGAT+GGG | + | chr4.2:75772631-75772650 | MS.gene82203:CDS | 30.0% | |
| GCAAAAGATTACTAAAGCTA+GGG | + | chr4.2:75772686-75772705 | MS.gene82203:CDS | 30.0% | |
| GGTTTCTGATTATAAAGACT+TGG | + | chr4.2:75772806-75772825 | MS.gene82203:CDS | 30.0% | |
| TGCAAAAGATTACTAAAGCT+AGG | + | chr4.2:75772685-75772704 | MS.gene82203:CDS | 30.0% | |
| TTTGCAAAAGGAAACTTTCT+TGG | - | chr4.2:75772671-75772690 | None:intergenic | 30.0% | |
| ! | TGTTTAGCTTCCTTTAGATT+TGG | - | chr4.2:75772594-75772613 | None:intergenic | 30.0% |
| !!! | TGTTTTAGAATCGAAAGGAT+CGG | - | chr4.2:75772562-75772581 | None:intergenic | 30.0% |
| AAGCTAAACAGATGATTGAG+AGG | + | chr4.2:75772604-75772623 | MS.gene82203:CDS | 35.0% | |
| AGAGAACTCACTATTCTTTG+TGG | + | chr4.2:75772513-75772532 | MS.gene82203:CDS | 35.0% | |
| AGCTGAACATTCGTATGATT+TGG | + | chr4.2:75772748-75772767 | MS.gene82203:CDS | 35.0% | |
| CAGAGCTCATATATCAAAGA+TGG | + | chr4.2:75772630-75772649 | MS.gene82203:CDS | 35.0% | |
| !! | GAAGAAAGGTATCATCAAGA+AGG | + | chr4.2:75772488-75772507 | MS.gene82203:CDS | 35.0% |
| !! | TCCTTTCGATTCTAAAACAG+AGG | + | chr4.2:75772563-75772582 | MS.gene82203:CDS | 35.0% |
| !!! | ACCTCTGTTTTAGAATCGAA+AGG | - | chr4.2:75772567-75772586 | None:intergenic | 35.0% |
| TCGGAAATTATAGCACATGC+TGG | - | chr4.2:75772543-75772562 | None:intergenic | 40.0% | |
| !! | GAAGGAAGTTGATGACAAGA+TGG | + | chr4.2:75772851-75772870 | MS.gene82203:CDS | 40.0% |
| !! | TCGATTCTAAAACAGAGGTG+TGG | + | chr4.2:75772568-75772587 | MS.gene82203:CDS | 40.0% |
| TCAGAAACCGTCACATCATC+TGG | - | chr4.2:75772795-75772814 | None:intergenic | 45.0% | |
| ! | AGAGGTGTGGCCAAATCTAA+AGG | + | chr4.2:75772581-75772600 | MS.gene82203:CDS | 45.0% |
| !!! | CTGGCAGCTTGTTGTTTTGT+AGG | - | chr4.2:75772776-75772795 | None:intergenic | 45.0% |
| CAAGCTGCCAGATGATGTGA+CGG | + | chr4.2:75772785-75772804 | MS.gene82203:CDS | 50.0% | |
| ACCTGCAAGCGGAGGAAGAA+AGG | + | chr4.2:75772474-75772493 | MS.gene82203:CDS | 55.0% | |
| ACCTTTCTTCCTCCGCTTGC+AGG | - | chr4.2:75772478-75772497 | None:intergenic | 55.0% | |
| CAAGAAAGGCGACCTGCAAG+CGG | + | chr4.2:75772463-75772482 | MS.gene82203:CDS | 55.0% | |
| GAAAGGCGACCTGCAAGCGG+AGG | + | chr4.2:75772466-75772485 | MS.gene82203:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 75772417 | 75772896 | 75772417 | ID=MS.gene82203 |
| chr4.2 | mRNA | 75772417 | 75772896 | 75772417 | ID=MS.gene82203.t1;Parent=MS.gene82203 |
| chr4.2 | exon | 75772417 | 75772896 | 75772417 | ID=MS.gene82203.t1.exon1;Parent=MS.gene82203.t1 |
| chr4.2 | CDS | 75772417 | 75772896 | 75772417 | ID=cds.MS.gene82203.t1;Parent=MS.gene82203.t1 |
| Gene Sequence |
| Protein sequence |
