Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82205.t1 | XP_003605334.1 | 96.9 | 159 | 5 | 0 | 1 | 159 | 1 | 159 | 1.10E-77 | 299.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82205.t1 | Q9FJK3 | 46.2 | 156 | 83 | 1 | 1 | 155 | 1 | 156 | 7.1e-30 | 131.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82205.t1 | G7JJI5 | 96.9 | 159 | 5 | 0 | 1 | 159 | 1 | 159 | 7.7e-78 | 299.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene82205.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82205.t1 | MTR_4g028720 | 96.855 | 159 | 5 | 0 | 1 | 159 | 1 | 159 | 3.92e-112 | 315 |
| MS.gene82205.t1 | MTR_7g011950 | 90.566 | 159 | 15 | 0 | 1 | 159 | 1 | 159 | 2.67e-97 | 278 |
| MS.gene82205.t1 | MTR_1g077320 | 84.906 | 159 | 24 | 0 | 1 | 159 | 1 | 159 | 8.21e-90 | 258 |
| MS.gene82205.t1 | MTR_5g047580 | 69.872 | 156 | 47 | 0 | 1 | 156 | 1 | 156 | 3.45e-75 | 221 |
| MS.gene82205.t1 | MTR_1g077390 | 69.427 | 157 | 48 | 0 | 1 | 157 | 1 | 157 | 3.69e-75 | 221 |
| MS.gene82205.t1 | MTR_1g084950 | 75.641 | 156 | 38 | 0 | 1 | 156 | 1 | 156 | 2.65e-74 | 219 |
| MS.gene82205.t1 | MTR_1g077300 | 73.585 | 159 | 40 | 1 | 1 | 159 | 1 | 157 | 5.79e-71 | 211 |
| MS.gene82205.t1 | MTR_1g090697 | 55.484 | 155 | 69 | 0 | 1 | 155 | 1 | 155 | 7.63e-58 | 177 |
| MS.gene82205.t1 | MTR_5g047560 | 58.333 | 156 | 49 | 1 | 1 | 156 | 1 | 140 | 1.91e-56 | 173 |
| MS.gene82205.t1 | MTR_1g090710 | 53.846 | 156 | 71 | 1 | 1 | 155 | 1 | 156 | 4.45e-56 | 173 |
| MS.gene82205.t1 | MTR_1g090783 | 54.248 | 153 | 70 | 0 | 3 | 155 | 2 | 154 | 1.34e-54 | 169 |
| MS.gene82205.t1 | MTR_4g032620 | 40.645 | 155 | 90 | 2 | 1 | 153 | 1 | 155 | 5.29e-42 | 140 |
| MS.gene82205.t1 | MTR_3g466980 | 45.098 | 153 | 83 | 1 | 1 | 152 | 1 | 153 | 7.84e-41 | 136 |
| MS.gene82205.t1 | MTR_3g065100 | 47.134 | 157 | 82 | 1 | 1 | 156 | 1 | 157 | 3.39e-38 | 130 |
| MS.gene82205.t1 | MTR_5g075380 | 37.821 | 156 | 96 | 1 | 1 | 155 | 1 | 156 | 4.71e-35 | 120 |
| MS.gene82205.t1 | MTR_3g466830 | 41.401 | 157 | 91 | 1 | 3 | 158 | 2 | 158 | 3.64e-34 | 117 |
| MS.gene82205.t1 | MTR_8g036130 | 38.365 | 159 | 94 | 2 | 1 | 155 | 1 | 159 | 9.20e-34 | 119 |
| MS.gene82205.t1 | MTR_4g063790 | 37.107 | 159 | 98 | 2 | 1 | 157 | 1 | 159 | 9.80e-33 | 114 |
| MS.gene82205.t1 | MTR_3g466890 | 41.558 | 154 | 89 | 1 | 3 | 155 | 2 | 155 | 1.83e-32 | 113 |
| MS.gene82205.t1 | MTR_4g031910 | 42.000 | 150 | 85 | 2 | 1 | 148 | 1 | 150 | 1.16e-31 | 113 |
| MS.gene82205.t1 | MTR_4g032260 | 41.875 | 160 | 91 | 2 | 1 | 158 | 1 | 160 | 7.82e-31 | 109 |
| MS.gene82205.t1 | MTR_3g467080 | 34.161 | 161 | 104 | 2 | 1 | 159 | 1 | 161 | 2.62e-30 | 108 |
| MS.gene82205.t1 | MTR_2g016210 | 38.994 | 159 | 95 | 2 | 1 | 157 | 1 | 159 | 9.11e-30 | 108 |
| MS.gene82205.t1 | MTR_4g032290 | 39.752 | 161 | 95 | 2 | 1 | 159 | 1 | 161 | 1.96e-28 | 105 |
| MS.gene82205.t1 | MTR_3g031240 | 33.333 | 159 | 104 | 2 | 1 | 157 | 1 | 159 | 7.48e-28 | 102 |
| MS.gene82205.t1 | MTR_3g031100 | 34.395 | 157 | 101 | 2 | 1 | 155 | 1 | 157 | 6.73e-27 | 101 |
| MS.gene82205.t1 | MTR_2g035580 | 36.129 | 155 | 98 | 1 | 1 | 154 | 1 | 155 | 2.80e-25 | 95.1 |
| MS.gene82205.t1 | MTR_2g035610 | 41.525 | 118 | 68 | 1 | 1 | 117 | 1 | 118 | 2.75e-24 | 91.7 |
| MS.gene82205.t1 | MTR_3g466930 | 37.600 | 125 | 77 | 1 | 32 | 155 | 2 | 126 | 8.87e-21 | 82.8 |
| MS.gene82205.t1 | MTR_3g466900 | 38.400 | 125 | 76 | 1 | 32 | 155 | 2 | 126 | 1.52e-20 | 82.0 |
| MS.gene82205.t1 | MTR_3g465410 | 33.588 | 131 | 85 | 2 | 29 | 157 | 1 | 131 | 1.92e-20 | 82.4 |
| MS.gene82205.t1 | MTR_4g019670 | 37.008 | 127 | 79 | 1 | 3 | 128 | 6 | 132 | 4.67e-20 | 86.3 |
| MS.gene82205.t1 | MTR_4g028800 | 45.833 | 72 | 37 | 1 | 1 | 72 | 1 | 70 | 1.18e-19 | 78.2 |
| MS.gene82205.t1 | MTR_7g106510 | 38.889 | 108 | 65 | 1 | 3 | 109 | 6 | 113 | 3.07e-19 | 83.6 |
| MS.gene82205.t1 | MTR_7g055800 | 31.624 | 117 | 53 | 2 | 1 | 117 | 1 | 90 | 5.06e-14 | 64.3 |
| MS.gene82205.t1 | MTR_3g093900 | 31.034 | 145 | 94 | 3 | 3 | 143 | 6 | 148 | 6.28e-14 | 68.6 |
| MS.gene82205.t1 | MTR_1g033370 | 42.553 | 94 | 53 | 1 | 64 | 156 | 28 | 121 | 2.81e-13 | 63.2 |
| MS.gene82205.t1 | MTR_1g033790 | 40.816 | 98 | 57 | 1 | 62 | 158 | 20 | 117 | 3.58e-13 | 62.8 |
| MS.gene82205.t1 | MTR_1g033930 | 38.776 | 98 | 59 | 1 | 62 | 158 | 20 | 117 | 3.73e-13 | 62.8 |
| MS.gene82205.t1 | MTR_6g005450 | 31.373 | 102 | 64 | 3 | 1 | 96 | 1 | 102 | 8.86e-12 | 62.0 |
| MS.gene82205.t1 | MTR_7g055940 | 33.654 | 104 | 42 | 3 | 1 | 104 | 1 | 77 | 2.26e-11 | 57.0 |
| MS.gene82205.t1 | MTR_6g018920 | 26.316 | 133 | 76 | 5 | 1 | 114 | 1 | 130 | 9.25e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82205.t1 | AT5G48670 | 46.405 | 153 | 81 | 1 | 1 | 152 | 1 | 153 | 1.49e-34 | 123 |
| MS.gene82205.t1 | AT3G05860 | 38.926 | 149 | 90 | 1 | 1 | 148 | 1 | 149 | 9.82e-32 | 113 |
| MS.gene82205.t1 | AT3G05860 | 38.926 | 149 | 90 | 1 | 1 | 148 | 1 | 149 | 4.70e-31 | 112 |
| MS.gene82205.t1 | AT3G05860 | 38.926 | 149 | 90 | 1 | 1 | 148 | 1 | 149 | 6.97e-31 | 112 |
| MS.gene82205.t1 | AT5G26630 | 38.750 | 160 | 97 | 1 | 1 | 159 | 1 | 160 | 2.35e-29 | 107 |
| MS.gene82205.t1 | AT1G65300 | 35.915 | 142 | 90 | 1 | 3 | 143 | 2 | 143 | 1.10e-27 | 104 |
| MS.gene82205.t1 | AT1G65330 | 36.735 | 147 | 92 | 1 | 3 | 148 | 2 | 148 | 7.62e-27 | 102 |
| MS.gene82205.t1 | AT5G26650 | 33.333 | 153 | 101 | 1 | 4 | 155 | 2 | 154 | 1.04e-24 | 98.2 |
| MS.gene82205.t1 | AT5G27960 | 32.680 | 153 | 102 | 1 | 4 | 155 | 2 | 154 | 1.98e-23 | 94.0 |
| MS.gene82205.t1 | AT1G22590 | 30.247 | 162 | 97 | 3 | 1 | 155 | 1 | 153 | 8.31e-23 | 89.0 |
| MS.gene82205.t1 | AT1G31630 | 29.677 | 155 | 107 | 2 | 3 | 156 | 2 | 155 | 1.90e-21 | 89.0 |
| MS.gene82205.t1 | AT5G27810 | 42.708 | 96 | 54 | 1 | 29 | 123 | 1 | 96 | 1.14e-20 | 82.0 |
| MS.gene82205.t1 | AT2G28700 | 33.553 | 152 | 94 | 3 | 1 | 148 | 1 | 149 | 1.36e-20 | 86.7 |
| MS.gene82205.t1 | AT1G31640 | 32.000 | 150 | 100 | 2 | 3 | 151 | 2 | 150 | 5.01e-20 | 85.9 |
| MS.gene82205.t1 | AT5G26580 | 30.968 | 155 | 94 | 1 | 1 | 155 | 1 | 142 | 2.95e-18 | 80.1 |
| MS.gene82205.t1 | AT5G06500 | 31.646 | 158 | 106 | 2 | 1 | 157 | 1 | 157 | 2.78e-16 | 73.6 |
| MS.gene82205.t1 | AT2G40210 | 35.000 | 140 | 85 | 3 | 1 | 137 | 1 | 137 | 1.76e-15 | 72.8 |
| MS.gene82205.t1 | AT5G58890 | 32.479 | 117 | 70 | 3 | 1 | 108 | 1 | 117 | 4.36e-13 | 65.5 |
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGCAAAAGGAAACTTTCT+TGG | 0.256030 | 4.2:-75748217 | None:intergenic |
| AGCTGAACATTCTTATGATT+TGG | 0.274450 | 4.2:+75748297 | MS.gene82205:CDS |
| TCATCTGTTTGGCTTCCTTT+AGG | 0.279952 | 4.2:-75748145 | None:intergenic |
| CGCCTTTCTTGTTGAATCAT+TGG | 0.287726 | 4.2:-75748000 | None:intergenic |
| ACCTTTCTTCCTCCGCTTGT+AGG | 0.316491 | 4.2:-75748024 | None:intergenic |
| TGTTGAATCATTGGAGATAA+AGG | 0.341938 | 4.2:-75747991 | None:intergenic |
| ATACCTCTCAATCATCTGTT+TGG | 0.362514 | 4.2:-75748156 | None:intergenic |
| TGTTTGGCTTCCTTTAGGTT+TGG | 0.378359 | 4.2:-75748140 | None:intergenic |
| CAGAGCTCATATATGAAAGA+TGG | 0.456034 | 4.2:+75748179 | MS.gene82205:CDS |
| CTCCAATGATTCAACAAGAA+AGG | 0.460238 | 4.2:+75747998 | MS.gene82205:CDS |
| TGTTTCTGATTTGAAAGACT+TGG | 0.473410 | 4.2:+75748355 | MS.gene82205:CDS |
| TGCAAAAGATTACTAAAGCT+AGG | 0.481563 | 4.2:+75748234 | MS.gene82205:CDS |
| GCAAAAGATTACTAAAGCTA+GGG | 0.487712 | 4.2:+75748235 | MS.gene82205:CDS |
| ACCTACAAGCGGAGGAAGAA+AGG | 0.494539 | 4.2:+75748023 | MS.gene82205:CDS |
| CAAAGTCATGGCTAGGAAGA+AGG | 0.502352 | 4.2:+75747959 | None:intergenic |
| TCGAAAATTATAGCACATGC+TGG | 0.519544 | 4.2:-75748089 | None:intergenic |
| AGAGAACTCACTATTCTTTG+TGG | 0.526508 | 4.2:+75748062 | MS.gene82205:CDS |
| AGAGGTGTGGCCAAACCTAA+AGG | 0.549410 | 4.2:+75748130 | MS.gene82205:CDS |
| GAAGGAAATTGATGACAAGA+TGG | 0.549956 | 4.2:+75748400 | MS.gene82205:CDS |
| AGAGCTCATATATGAAAGAT+GGG | 0.560272 | 4.2:+75748180 | MS.gene82205:CDS |
| TACATTGCAAAGTCATGGCT+AGG | 0.590557 | 4.2:+75747952 | None:intergenic |
| GAAGAAAGGTATCATCAAGA+AGG | 0.595296 | 4.2:+75748037 | MS.gene82205:CDS |
| GAAAGGCGACCTACAAGCGG+AGG | 0.629626 | 4.2:+75748015 | MS.gene82205:CDS |
| AAGCCAAACAGATGATTGAG+AGG | 0.630265 | 4.2:+75748153 | MS.gene82205:CDS |
| TTGATTCTAAAACAGAGGTG+TGG | 0.633348 | 4.2:+75748117 | MS.gene82205:CDS |
| CAAGAAAGGCGACCTACAAG+CGG | 0.654193 | 4.2:+75748012 | MS.gene82205:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTGATTGAAAAAAATATGA+AGG | + | chr4.2:75748382-75748401 | MS.gene82205:CDS | 20.0% |
| !! | CTTTAGTAATCTTTTGCAAA+AGG | - | chr4.2:75748232-75748251 | None:intergenic | 25.0% |
| AGAGCTCATATATGAAAGAT+GGG | + | chr4.2:75748180-75748199 | MS.gene82205:CDS | 30.0% | |
| AGCTGAACATTCTTATGATT+TGG | + | chr4.2:75748297-75748316 | MS.gene82205:CDS | 30.0% | |
| GCAAAAGATTACTAAAGCTA+GGG | + | chr4.2:75748235-75748254 | MS.gene82205:CDS | 30.0% | |
| TGCAAAAGATTACTAAAGCT+AGG | + | chr4.2:75748234-75748253 | MS.gene82205:CDS | 30.0% | |
| TGTTGAATCATTGGAGATAA+AGG | - | chr4.2:75747994-75748013 | None:intergenic | 30.0% | |
| TTTGCAAAAGGAAACTTTCT+TGG | - | chr4.2:75748220-75748239 | None:intergenic | 30.0% | |
| ! | TGTTTCTGATTTGAAAGACT+TGG | + | chr4.2:75748355-75748374 | MS.gene82205:CDS | 30.0% |
| !!! | ACCTCTGTTTTAGAATCAAA+AGG | - | chr4.2:75748116-75748135 | None:intergenic | 30.0% |
| !!! | TCCTTTTGATTCTAAAACAG+AGG | + | chr4.2:75748112-75748131 | MS.gene82205:CDS | 30.0% |
| AGAGAACTCACTATTCTTTG+TGG | + | chr4.2:75748062-75748081 | MS.gene82205:CDS | 35.0% | |
| ATACCTCTCAATCATCTGTT+TGG | - | chr4.2:75748159-75748178 | None:intergenic | 35.0% | |
| CAGAGCTCATATATGAAAGA+TGG | + | chr4.2:75748179-75748198 | MS.gene82205:CDS | 35.0% | |
| CTCCAATGATTCAACAAGAA+AGG | + | chr4.2:75747998-75748017 | MS.gene82205:CDS | 35.0% | |
| TCGAAAATTATAGCACATGC+TGG | - | chr4.2:75748092-75748111 | None:intergenic | 35.0% | |
| ! | GAAGGAAATTGATGACAAGA+TGG | + | chr4.2:75748400-75748419 | MS.gene82205:CDS | 35.0% |
| !! | GAAGAAAGGTATCATCAAGA+AGG | + | chr4.2:75748037-75748056 | MS.gene82205:CDS | 35.0% |
| !! | TTGATTCTAAAACAGAGGTG+TGG | + | chr4.2:75748117-75748136 | MS.gene82205:CDS | 35.0% |
| AAGCCAAACAGATGATTGAG+AGG | + | chr4.2:75748153-75748172 | MS.gene82205:CDS | 40.0% | |
| CGCCTTTCTTGTTGAATCAT+TGG | - | chr4.2:75748003-75748022 | None:intergenic | 40.0% | |
| ! | TCATCTGTTTGGCTTCCTTT+AGG | - | chr4.2:75748148-75748167 | None:intergenic | 40.0% |
| ! | TGTTTGGCTTCCTTTAGGTT+TGG | - | chr4.2:75748143-75748162 | None:intergenic | 40.0% |
| !!! | CTGACAGCTTGTTGTTTTGT+AGG | - | chr4.2:75748325-75748344 | None:intergenic | 40.0% |
| ACCTACAAGCGGAGGAAGAA+AGG | + | chr4.2:75748023-75748042 | MS.gene82205:CDS | 50.0% | |
| ACCTTTCTTCCTCCGCTTGT+AGG | - | chr4.2:75748027-75748046 | None:intergenic | 50.0% | |
| CAAGAAAGGCGACCTACAAG+CGG | + | chr4.2:75748012-75748031 | MS.gene82205:CDS | 50.0% | |
| ! | AGAGGTGTGGCCAAACCTAA+AGG | + | chr4.2:75748130-75748149 | MS.gene82205:CDS | 50.0% |
| GAAAGGCGACCTACAAGCGG+AGG | + | chr4.2:75748015-75748034 | MS.gene82205:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 75747966 | 75748445 | 75747966 | ID=MS.gene82205 |
| chr4.2 | mRNA | 75747966 | 75748445 | 75747966 | ID=MS.gene82205.t1;Parent=MS.gene82205 |
| chr4.2 | exon | 75747966 | 75748445 | 75747966 | ID=MS.gene82205.t1.exon1;Parent=MS.gene82205.t1 |
| chr4.2 | CDS | 75747966 | 75748445 | 75747966 | ID=cds.MS.gene82205.t1;Parent=MS.gene82205.t1 |
| Gene Sequence |
| Protein sequence |
