AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82266


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82266.t1 MTR_5g082700 88.066 243 21 1 1 235 347 589 1.11e-147 426
MS.gene82266.t1 MTR_5g082700 88.066 243 21 1 1 235 347 589 8.72e-147 426
MS.gene82266.t1 MTR_5g082700 88.066 243 21 1 1 235 347 589 1.46e-146 426
MS.gene82266.t1 MTR_6g074905 57.080 226 53 2 18 235 859 1048 5.17e-79 258
MS.gene82266.t1 MTR_6g074870 52.586 232 55 4 12 235 95 279 3.38e-70 219
MS.gene82266.t1 MTR_0349s0030 73.913 115 30 0 121 235 54 168 2.67e-57 184
MS.gene82266.t1 MTR_8g069620 58.228 158 58 1 27 176 14 171 7.85e-55 175
MS.gene82266.t1 MTR_6g074640 50.877 171 46 2 37 207 11 143 4.75e-49 161
MS.gene82266.t1 MTR_8g070930 39.583 240 120 7 15 235 91 324 5.49e-44 158
MS.gene82266.t1 MTR_6g074750 75.000 80 20 0 156 235 18 97 2.98e-38 130
MS.gene82266.t1 MTR_1g111180 33.838 198 112 6 52 235 671 863 2.77e-24 101
MS.gene82266.t1 MTR_1g111180 33.838 198 112 6 52 235 670 862 2.96e-24 101
MS.gene82266.t1 MTR_3g076540 27.907 215 117 5 53 235 90 298 6.96e-19 85.5
MS.gene82266.t1 MTR_3g076540 27.907 215 117 5 53 235 8 216 7.02e-19 84.7
MS.gene82266.t1 MTR_4g104620 27.442 215 118 5 53 235 94 302 1.99e-18 84.0
MS.gene82266.t1 MTR_3g076540 28.571 182 102 3 77 235 23 199 1.35e-17 80.9
MS.gene82266.t1 MTR_4g104620 28.415 183 103 3 76 235 22 199 2.19e-17 80.1
MS.gene82266.t1 MTR_4g104620 28.415 183 103 3 76 235 31 208 2.75e-17 80.1
MS.gene82266.t1 MTR_3g076540 27.670 206 111 5 53 226 90 289 6.73e-17 79.7
MS.gene82266.t1 MTR_3g109390 27.103 214 119 5 53 235 70 277 8.32e-17 78.6
MS.gene82266.t1 MTR_3g109390 27.064 218 122 5 49 235 44 255 9.05e-17 79.0
MS.gene82266.t1 MTR_3g109390 27.064 218 122 5 49 235 66 277 9.23e-17 79.0
MS.gene82266.t1 MTR_3g109390 27.064 218 122 5 49 235 66 277 9.79e-17 79.0
MS.gene82266.t1 MTR_3g086530 24.444 225 128 6 46 235 82 299 2.55e-16 77.0
MS.gene82266.t1 MTR_3g086530 24.444 225 128 6 46 235 82 299 3.28e-16 77.4
MS.gene82266.t1 MTR_3g086530 24.444 225 128 6 46 235 82 299 3.87e-16 77.4
MS.gene82266.t1 MTR_3g109390 27.624 181 104 3 77 235 111 286 1.01e-14 73.2
MS.gene82266.t1 MTR_3g109390 27.624 181 104 3 77 235 89 264 1.02e-14 73.2
MS.gene82266.t1 MTR_3g086530 25.946 185 107 4 76 235 3 182 1.10e-14 72.4
MS.gene82266.t1 MTR_4g056390 34.932 146 78 5 57 189 39 180 1.52e-12 67.0
MS.gene82266.t1 MTR_2g084555 36.134 119 67 3 76 189 67 181 4.00e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82266.t1 AT2G40120 75.566 221 46 1 23 235 227 447 1.58e-117 347
MS.gene82266.t1 AT1G73460 67.699 226 65 1 18 235 819 1044 2.80e-104 327
MS.gene82266.t1 AT1G73460 67.699 226 65 1 18 235 819 1044 2.80e-104 327
MS.gene82266.t1 AT1G73450 67.699 226 65 1 18 235 802 1027 2.80e-104 327
MS.gene82266.t1 AT3G17750 67.257 226 66 1 18 235 788 1013 2.71e-102 321
MS.gene82266.t1 AT5G35980 40.271 221 108 7 33 235 92 306 1.61e-42 154
MS.gene82266.t1 AT5G35980 40.271 221 108 7 33 235 57 271 2.04e-42 154
MS.gene82266.t1 AT5G35980 40.271 221 108 7 33 235 92 306 2.09e-42 154
MS.gene82266.t1 AT1G13350 29.851 201 122 5 49 235 507 702 1.49e-20 90.9
MS.gene82266.t1 AT1G13350 32.716 162 102 4 76 235 473 629 1.81e-20 90.5
MS.gene82266.t1 AT3G53640 33.540 161 102 3 76 235 354 510 2.01e-20 90.5
MS.gene82266.t1 AT1G13350 32.716 162 102 4 76 235 500 656 2.15e-20 90.5
MS.gene82266.t1 AT3G25840 30.928 194 115 6 56 235 354 542 1.04e-19 88.2
MS.gene82266.t1 AT3G25840 32.298 161 102 4 77 235 649 804 1.15e-18 85.5
MS.gene82266.t1 AT4G24740 26.977 215 119 5 53 235 94 302 8.26e-18 81.3
MS.gene82266.t1 AT4G24740 26.977 215 119 5 53 235 94 302 9.01e-18 81.6
MS.gene82266.t1 AT4G24740 26.977 215 119 5 53 235 56 264 1.27e-17 81.6
MS.gene82266.t1 AT4G24740 26.977 215 119 5 53 235 94 302 1.56e-17 81.6
MS.gene82266.t1 AT3G53570 25.561 223 128 5 46 235 104 321 1.86e-17 81.6
MS.gene82266.t1 AT3G53570 25.561 223 128 5 46 235 104 321 1.86e-17 81.6
MS.gene82266.t1 AT3G53570 25.561 223 128 5 46 235 104 321 1.86e-17 81.6
MS.gene82266.t1 AT3G53570 25.561 223 128 5 46 235 90 307 1.93e-17 81.3
MS.gene82266.t1 AT3G53570 25.561 223 128 5 46 235 90 307 1.93e-17 81.3
MS.gene82266.t1 AT4G24740 28.022 182 103 3 77 235 23 199 6.24e-17 77.8
MS.gene82266.t1 AT4G24740 28.022 182 103 3 77 235 23 199 1.44e-16 78.2
MS.gene82266.t1 AT4G24740 28.022 182 103 3 77 235 23 199 1.44e-16 78.2
MS.gene82266.t1 AT4G24740 28.022 182 103 3 77 235 23 199 1.44e-16 78.2
MS.gene82266.t1 AT4G32660 28.972 214 115 5 53 235 67 274 4.91e-16 76.6
MS.gene82266.t1 AT4G32660 28.972 214 115 5 53 235 67 274 6.07e-16 76.6
MS.gene82266.t1 AT4G32660 28.972 214 115 5 53 235 59 266 6.72e-16 76.6
MS.gene82266.t1 AT2G17530 37.288 118 65 3 77 189 66 179 2.08e-12 66.2
MS.gene82266.t1 AT2G17530 37.288 118 65 3 77 189 66 179 4.81e-12 65.5
MS.gene82266.t1 AT2G17530 37.288 118 65 3 77 189 66 179 4.81e-12 65.5
MS.gene82266.t1 AT4G32660 30.303 165 88 3 93 235 3 162 6.44e-12 64.3
MS.gene82266.t1 AT3G44850 36.975 119 66 4 76 189 72 186 3.63e-11 63.2

Find 0 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAAAAGTTACAAAAAAT+GGG - 42454:10210-10229 MS.gene82266:CDS 15.0%
!! TAATCAAAAGTTACAAAAAA+TGG - 42454:10209-10228 MS.gene82266:CDS 15.0%
!! ATTGCAATATTTACATAACT+TGG - 42454:10150-10169 MS.gene82266:CDS 20.0%
!! GAAGATTATTAAAAATGACA+AGG - 42454:9883-9902 MS.gene82266:CDS 20.0%
!! TTGCAATATTTACATAACTT+GGG - 42454:10151-10170 MS.gene82266:CDS 20.0%
!!! CAATTTTTTCATCATATTGA+AGG + 42454:10336-10355 MS.gene82266:intergenic 20.0%
CAATGAATAAATGTTCCTGA+TGG + 42454:10012-10031 MS.gene82266:intergenic 30.0%
TAAGAAGCTTGATTTCATCT+AGG + 42454:9925-9944 MS.gene82266:intergenic 30.0%
TACTATGTGACAGATTATCT+CGG - 42454:9821-9840 MS.gene82266:CDS 30.0%
! TCAATCAATTGAGTTGTGTA+AGG + 42454:10379-10398 MS.gene82266:intergenic 30.0%
!! CTTTTGATTAGAACGTTTTC+AGG + 42454:10199-10218 MS.gene82266:intergenic 30.0%
!! TTATGACTATTTCTACCATC+AGG - 42454:9994-10013 MS.gene82266:CDS 30.0%
AAATTCAACCAAGAATCTGG+TGG - 42454:10070-10089 MS.gene82266:CDS 35.0%
ATTATTATCCTCTCCATCTG+TGG + 42454:9660-9679 MS.gene82266:intergenic 35.0%
CAGAAATTCAACCAAGAATC+TGG - 42454:10067-10086 MS.gene82266:CDS 35.0%
CATCTTGACTGTATTTCAAG+AGG + 42454:9694-9713 MS.gene82266:intergenic 35.0%
GACTGGATTTGAAGAAAACA+AGG - 42454:9760-9779 MS.gene82266:CDS 35.0%
TGTGTCTATATGTACAATCC+CGG - 42454:10281-10300 MS.gene82266:CDS 35.0%
TTTGAGCTAAGAATTGTGCA+TGG - 42454:9728-9747 MS.gene82266:CDS 35.0%
! ATTGATGACAGAACACCTTT+AGG - 42454:10352-10371 MS.gene82266:CDS 35.0%
!! TTAGAACGTTTTCAGGCTTT+AGG + 42454:10192-10211 MS.gene82266:intergenic 35.0%
AGAGCTTCTGATGTGATGTT+AGG - 42454:10310-10329 MS.gene82266:CDS 40.0%
AGTATGTGGTGTTTATCTCC+CGG + 42454:9968-9987 MS.gene82266:intergenic 40.0%
AGTCATAAAGACGCAGTATG+TGG + 42454:9982-10001 MS.gene82266:intergenic 40.0%
ATCAGAAGCTCTATAAGACC+GGG + 42454:10302-10321 MS.gene82266:intergenic 40.0%
CATCAGAAGCTCTATAAGAC+CGG + 42454:10303-10322 MS.gene82266:intergenic 40.0%
GAATTGTGCATGGAAAGAAC+AGG - 42454:9738-9757 MS.gene82266:CDS 40.0%
GTTCATTACTCGACAGTGTT+TGG - 42454:10123-10142 MS.gene82266:CDS 40.0%
TAAGTTTGCACGCAGAAGTT+TGG + 42454:10038-10057 MS.gene82266:intergenic 40.0%
TGAGTTGTGTAAGGACCTAA+AGG + 42454:10370-10389 MS.gene82266:intergenic 40.0%
ATGCTTCACCACCAGATTCT+TGG + 42454:10081-10100 MS.gene82266:intergenic 45.0%
CATTACTCGACAGTGTTTGG+AGG - 42454:10126-10145 MS.gene82266:CDS 45.0%
CGGTTCAGCTACATTCAGTA+GGG - 42454:9841-9860 MS.gene82266:CDS 45.0%
GTATGTGGTGTTTATCTCCC+GGG + 42454:9967-9986 MS.gene82266:intergenic 45.0%
TCGGTTCAGCTACATTCAGT+AGG - 42454:9840-9859 MS.gene82266:CDS 45.0%
TTCAGTAGGGATGTTCTCGA+TGG - 42454:9854-9873 MS.gene82266:CDS 45.0%
!! AGTGCTGATTGTGCTGAACA+CGG - 42454:9784-9803 MS.gene82266:CDS 45.0%
ACTTGTCGATAAGCACGACC+CGG - 42454:9946-9965 MS.gene82266:CDS 50.0%
GATTGTGCTGAACACGGTCA+TGG - 42454:9790-9809 MS.gene82266:CDS 50.0%
GTGCATGGAAAGAACAGGAC+TGG - 42454:9743-9762 MS.gene82266:CDS 50.0%
TGAACACGGTCATGGCTGAA+AGG - 42454:9798-9817 MS.gene82266:CDS 50.0%
CTTGTCGATAAGCACGACCC+GGG - 42454:9947-9966 MS.gene82266:CDS 55.0%


Chromosome Type Strat End Strand Name
42454 gene 9653 10398 9653 ID=MS.gene82266
42454 mRNA 9653 10398 9653 ID=MS.gene82266.t1;Parent=MS.gene82266
42454 exon 9653 10398 9653 ID=MS.gene82266.t1.exon1;Parent=MS.gene82266.t1
42454 CDS 9653 10398 9653 ID=cds.MS.gene82266.t1;Parent=MS.gene82266.t1
Gene Sequence

>MS.gene82266

GCTGCCACAGATGGAGAGGATAATAATATTGATGATGACCTCTTGAAATACAGTCAAGATGATGAATATGAAGTATTTGAGCTAAGAATTGTGCATGGAAAGAACAGGACTGGATTTGAAGAAAACAAGGAAGTGCTGATTGTGCTGAACACGGTCATGGCTGAAAGGTACTATGTGACAGATTATCTCGGTTCAGCTACATTCAGTAGGGATGTTCTCGATGGTTGTTTGAAGATTATTAAAAATGACAAGGATTTCTTTCATCAAAGCCTAGATGAAATCAAGCTTCTTAAACTTGTCGATAAGCACGACCCGGGAGATAAACACCACATACTGCGTCTTTATGACTATTTCTACCATCAGGAACATTTATTCATTGTGACCAAACTTCTGCGTGCAAACTTATATGAATTTCAGAAATTCAACCAAGAATCTGGTGGTGAAGCATATTTTACATTGAAGAGATTGCAGTTCATTACTCGACAGTGTTTGGAGGCATTGCAATATTTACATAACTTGGGAATTGTTCACTGTGACCTAAAGCCTGAAAACGTTCTAATCAAAAGTTACAAAAAATGGGAAATCAAAATTATTGATCTTGTAAGCAGTTGCTTTAAAACAGACAATTTGTGTCTATATGTACAATCCCGGTCTTATAGAGCTTCTGATGTGATGTTAGGCCTTCAATATGATGAAAAAATTGATGACAGAACACCTTTAGGTCCTTACACAACTCAATTGATTGA

Protein sequence

>MS.gene82266.t1

AATDGEDNNIDDDLLKYSQDDEYEVFELRIVHGKNRTGFEENKEVLIVLNTVMAERYYVTDYLGSATFSRDVLDGCLKIIKNDKDFFHQSLDEIKLLKLVDKHDPGDKHHILRLYDYFYHQEHLFIVTKLLRANLYEFQKFNQESGGEAYFTLKRLQFITRQCLEALQYLHNLGIVHCDLKPENVLIKSYKKWEIKIIDLVSSCFKTDNLCLYVQSRSYRASDVMLGLQYDEKIDDRTPLGPYTTQLI