AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82292

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82292.t1 MTR_5g039760 73.077 182 25 1 1 182 1 158 4.66e-89 273
MS.gene82292.t1 MTR_7g074790 65.922 179 42 2 1 179 1 160 5.63e-75 238
MS.gene82292.t1 MTR_7g074790 63.158 76 24 3 492 566 155 227 6.18e-21 92.4
MS.gene82292.t1 MTR_7g061980 68.333 120 37 1 1 120 1 119 3.65e-53 177
MS.gene82292.t1 MTR_0402s0040 35.495 293 142 8 10 300 26 273 3.63e-44 164
MS.gene82292.t1 MTR_3g058090 50.000 126 39 2 368 493 5 106 2.04e-28 109
MS.gene82292.t1 MTR_4g102720 31.053 190 93 4 7 196 4 155 7.42e-23 97.8
MS.gene82292.t1 MTR_4g069880 63.492 63 23 0 1 63 1 63 2.43e-21 88.2
MS.gene82292.t1 MTR_4g117620 74.000 50 13 0 517 566 27 76 2.46e-20 85.9
MS.gene82292.t1 MTR_5g015480 41.463 123 69 3 10 130 56 177 1.19e-18 86.3
MS.gene82292.t1 MTR_2g070590 41.905 105 58 3 10 112 17 120 9.54e-18 79.7
MS.gene82292.t1 MTR_2g099430 48.315 89 43 3 33 120 26 112 2.73e-17 78.6
MS.gene82292.t1 MTR_6g051425 29.101 189 122 5 9 187 13 199 1.43e-16 81.6
MS.gene82292.t1 MTR_1g019850 32.624 141 87 3 3 142 63 196 1.00e-15 77.4
MS.gene82292.t1 MTR_6g017200 44.186 86 46 2 10 94 58 142 3.75e-15 73.6
MS.gene82292.t1 MTR_1g013060 29.412 170 115 2 7 171 21 190 1.45e-13 70.9
MS.gene82292.t1 MTR_7g113010 37.008 127 65 3 10 135 457 569 3.35e-13 72.8
MS.gene82292.t1 MTR_5g076070 30.625 160 109 2 13 170 56 215 2.43e-12 68.2
MS.gene82292.t1 MTR_4g067290 26.702 191 104 5 9 197 55 211 2.72e-12 67.4
MS.gene82292.t1 MTR_2g014100 26.425 193 120 5 8 181 45 234 3.58e-12 67.0
MS.gene82292.t1 MTR_4g067290 26.702 191 104 5 9 197 75 231 5.85e-12 66.6
MS.gene82292.t1 MTR_4g067290 36.364 110 66 3 9 117 55 161 7.63e-12 65.9
MS.gene82292.t1 MTR_4g067290 36.364 110 66 3 9 117 75 181 1.18e-11 65.9
MS.gene82292.t1 MTR_4g067290 36.364 110 66 3 9 117 73 179 1.18e-11 65.5
MS.gene82292.t1 MTR_4g067290 26.702 191 104 5 9 197 115 271 1.26e-11 66.2
MS.gene82292.t1 MTR_1g057920 39.535 86 50 2 10 94 34 118 1.47e-11 62.4
MS.gene82292.t1 MTR_0734s0010 29.947 187 102 7 360 526 1 178 1.89e-11 63.5
MS.gene82292.t1 MTR_0558s0020 26.425 193 120 5 8 181 37 226 2.16e-11 65.5
MS.gene82292.t1 MTR_1g083640 25.907 193 121 5 8 181 52 241 6.95e-11 63.9
MS.gene82292.t1 MTR_5g045210 31.765 85 58 0 370 454 20 104 9.06e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82292.t1 AT4G38180 30.137 219 149 3 183 399 424 640 1.07e-33 137
MS.gene82292.t1 AT4G38180 31.068 206 138 3 6 208 69 273 1.16e-22 103
MS.gene82292.t1 AT4G15090 24.297 391 261 10 183 567 400 761 4.34e-32 132
MS.gene82292.t1 AT4G15090 26.705 176 125 4 3 176 44 217 5.72e-12 69.3
MS.gene82292.t1 AT4G15090 24.297 391 261 10 183 567 400 761 4.34e-32 132
MS.gene82292.t1 AT4G15090 26.705 176 125 4 3 176 44 217 5.72e-12 69.3
MS.gene82292.t1 AT4G15090 24.297 391 261 10 183 567 400 761 4.34e-32 132
MS.gene82292.t1 AT4G15090 26.705 176 125 4 3 176 44 217 5.72e-12 69.3
MS.gene82292.t1 AT4G15090 24.297 391 261 10 183 567 400 761 4.34e-32 132
MS.gene82292.t1 AT4G15090 26.705 176 125 4 3 176 44 217 5.72e-12 69.3
MS.gene82292.t1 AT4G15090 24.297 391 261 10 183 567 400 761 4.34e-32 132
MS.gene82292.t1 AT4G15090 26.705 176 125 4 3 176 44 217 5.72e-12 69.3
MS.gene82292.t1 AT4G38170 26.269 335 232 8 165 495 174 497 7.83e-32 130
MS.gene82292.t1 AT3G22170 28.682 258 171 5 183 437 409 656 1.19e-30 127
MS.gene82292.t1 AT3G22170 28.682 258 171 5 183 437 409 656 1.19e-30 127
MS.gene82292.t1 AT1G76320 29.858 211 145 2 183 393 337 544 1.93e-28 120
MS.gene82292.t1 AT1G76320 29.858 211 145 2 183 393 337 544 1.93e-28 120
MS.gene82292.t1 AT1G76320 29.858 211 145 2 183 393 337 544 1.95e-28 120
MS.gene82292.t1 AT1G76320 25.879 313 215 6 183 493 337 634 3.21e-28 120
MS.gene82292.t1 AT5G18960 28.226 248 165 4 183 425 521 760 6.03e-28 119
MS.gene82292.t1 AT5G18960 38.462 104 49 2 9 111 42 131 3.13e-12 70.1
MS.gene82292.t1 AT5G18960 28.226 248 165 4 183 425 521 760 6.03e-28 119
MS.gene82292.t1 AT5G18960 38.462 104 49 2 9 111 42 131 3.13e-12 70.1
MS.gene82292.t1 AT3G06250 27.419 248 167 4 183 425 497 736 9.59e-28 119
MS.gene82292.t1 AT3G06250 37.500 104 50 2 9 111 27 116 1.27e-11 68.2
MS.gene82292.t1 AT3G06250 27.419 248 167 4 183 425 497 736 9.59e-28 119
MS.gene82292.t1 AT3G06250 37.500 104 50 2 9 111 27 116 1.27e-11 68.2
MS.gene82292.t1 AT3G06250 27.419 248 167 4 183 425 497 736 9.59e-28 119
MS.gene82292.t1 AT3G06250 37.500 104 50 2 9 111 27 116 1.27e-11 68.2
MS.gene82292.t1 AT4G19990 30.516 213 145 2 183 393 348 559 7.38e-27 116
MS.gene82292.t1 AT4G19990 30.516 213 145 2 183 393 348 559 7.38e-27 116
MS.gene82292.t1 AT4G19990 30.516 213 145 2 183 393 348 559 7.45e-27 115
MS.gene82292.t1 AT4G19990 30.516 213 145 2 183 393 348 559 7.45e-27 115
MS.gene82292.t1 AT4G19990 30.516 213 145 2 183 393 291 502 7.60e-27 115
MS.gene82292.t1 AT4G19990 30.516 213 145 2 183 393 348 559 8.13e-27 115
MS.gene82292.t1 AT4G19990 30.516 213 145 2 183 393 362 573 9.70e-27 115
MS.gene82292.t1 AT1G52520 25.909 220 153 3 183 393 420 638 4.00e-21 98.2
MS.gene82292.t1 AT2G32250 23.462 260 185 4 184 439 304 553 2.30e-19 92.8
MS.gene82292.t1 AT2G32250 23.462 260 185 4 184 439 304 553 2.35e-19 92.8
MS.gene82292.t1 AT2G32250 23.462 260 185 4 184 439 345 594 2.50e-19 92.8
MS.gene82292.t1 AT2G32250 23.462 260 185 4 184 439 345 594 2.50e-19 92.8
MS.gene82292.t1 AT2G32250 23.462 260 185 4 184 439 345 594 2.50e-19 92.8
MS.gene82292.t1 AT2G32250 23.462 260 185 4 184 439 345 594 2.53e-19 92.4
MS.gene82292.t1 AT2G32250 23.462 260 185 4 184 439 345 594 2.53e-19 92.4
MS.gene82292.t1 AT2G27110 25.292 257 183 5 183 437 226 475 2.76e-19 92.4
MS.gene82292.t1 AT2G27110 25.292 257 183 5 183 437 371 620 2.88e-19 92.4
MS.gene82292.t1 AT2G27110 25.292 257 183 5 183 437 371 620 2.88e-19 92.4
MS.gene82292.t1 AT2G32250 23.462 260 185 4 184 439 345 594 3.04e-19 92.4
MS.gene82292.t1 AT1G80010 26.950 282 190 9 183 451 445 723 6.19e-19 91.3
MS.gene82292.t1 AT1G10240 23.214 280 199 9 183 460 402 667 6.16e-13 72.0
MS.gene82292.t1 AT1G10240 23.214 280 199 9 183 460 402 667 6.16e-13 72.0
MS.gene82292.t1 AT3G59470 35.577 104 64 2 9 111 69 170 6.93e-11 63.5
MS.gene82292.t1 AT3G59470 35.577 104 64 2 9 111 69 170 6.93e-11 63.5

Find 89 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTCTTAATCCAGAGTTAT+TGG 0.153704 3.4:+22199670 None:intergenic
TGGAAAAGGCTAAACAATTT+TGG 0.157186 3.4:-22200024 MS.gene82292:CDS
CGAAATGATTGAAGAATTCT+AGG 0.180160 3.4:+22198805 None:intergenic
TAAGAATATGCTCCAATATT+TGG 0.196187 3.4:+22197680 None:intergenic
TCTTGGATCAAGACAACTTA+TGG 0.222245 3.4:-22198951 MS.gene82292:CDS
CCCTTTGACGATCCAAATAT+TGG 0.237193 3.4:-22197692 MS.gene82292:CDS
GAATTACACTTTCACTTTGT+AGG 0.262576 3.4:-22199820 MS.gene82292:CDS
TGGAATGGGATTTGATAATA+TGG 0.262817 3.4:-22200044 MS.gene82292:CDS
CTTCCATTGCTTGTAGTTGT+AGG 0.300092 3.4:-22198509 MS.gene82292:CDS
TTTCCTTAGTGATGACATAT+TGG 0.316925 3.4:+22198574 None:intergenic
TAACTACACAGCCATGTTAA+TGG 0.324178 3.4:-22197800 MS.gene82292:intron
ACCTGTGCACTTCTTTGTTT+AGG 0.326471 3.4:+22198015 None:intergenic
GAGTCAGTTTCATGTCTAAT+TGG 0.338630 3.4:+22198603 None:intergenic
TGTAGGAAGTTTGAGACATT+TGG 0.350416 3.4:-22198492 MS.gene82292:CDS
TCAAATCCCATTCCAACCTT+TGG 0.351708 3.4:+22200052 None:intergenic
CTTTGAATTGAATGATGTTA+AGG 0.355506 3.4:-22198442 MS.gene82292:CDS
CGGTGAAAACGGTGCTCGTC+CGG 0.369800 3.4:-22198163 MS.gene82292:CDS
CTTGGATCAAGACAACTTAT+GGG 0.391827 3.4:-22198950 MS.gene82292:CDS
TTCACCAATACTAACCCAAA+TGG 0.399541 3.4:-22198697 MS.gene82292:CDS
CTTCTTAAGATATACATATC+AGG 0.401674 3.4:+22198414 None:intergenic
ATAATATTCGTGATAAATCT+TGG 0.406498 3.4:-22198968 MS.gene82292:CDS
ACAAGCATATGAAATTGATA+TGG 0.409436 3.4:-22199693 MS.gene82292:CDS
TATGGTTTACGTGTGGGCTT+CGG 0.418417 3.4:-22199995 MS.gene82292:CDS
ATGTAGGTTCGTTTGTTGTA+AGG 0.422514 3.4:-22199924 MS.gene82292:CDS
ATTCGATTTCTTAACAACTT+AGG 0.424669 3.4:+22198327 None:intergenic
AAGGAAGGGGTGAAGAGTAC+AGG 0.424745 3.4:-22199905 MS.gene82292:CDS
ATCTTGCACAATGCCATAAC+GGG 0.434412 3.4:+22198377 None:intergenic
GGATCAGAACTCATAATTGC+CGG 0.442476 3.4:+22198144 None:intergenic
AATTCTAGGATATCCACATT+TGG 0.443045 3.4:+22198819 None:intergenic
TTTCCCATTTGGGTTAGTAT+TGG 0.448473 3.4:+22198693 None:intergenic
GGAATCTCGCGCACAAGCTA+TGG 0.451664 3.4:-22198193 MS.gene82292:CDS
ATTGATATGGCCAATAACTC+TGG 0.454000 3.4:-22199680 MS.gene82292:CDS
TAAAGGATTAACAGCTCATA+TGG 0.458478 3.4:-22197887 MS.gene82292:CDS
GGGATTTGATAATATGGAAA+AGG 0.467508 3.4:-22200038 MS.gene82292:CDS
TGGCATCTACAAATGTTGAT+TGG 0.468983 3.4:-22200078 MS.gene82292:CDS
TGTCGATCGCCGGTCAATCA+AGG 0.471310 3.4:+22197657 None:intergenic
TGGACAAGGGAAGCCCGTTA+TGG 0.472858 3.4:-22198390 MS.gene82292:CDS
GGTTCGTTTGTTGTAAGGAA+GGG 0.477120 3.4:-22199919 MS.gene82292:CDS
TCCAATATTTGGATCGTCAA+AGG 0.483053 3.4:+22197691 None:intergenic
TGGAAGCCAAAGGTTGGAAT+GGG 0.484209 3.4:-22200058 MS.gene82292:CDS
CTTGCTTATGGTTTACGTGT+GGG 0.490896 3.4:-22200001 MS.gene82292:CDS
TCACCAATACTAACCCAAAT+GGG 0.493652 3.4:-22198696 MS.gene82292:CDS
TGTGCACTTCTTTGTTTAGG+TGG 0.498449 3.4:+22198018 None:intergenic
AATCTTGCACAATGCCATAA+CGG 0.499131 3.4:+22198376 None:intergenic
AGGTTCGTTTGTTGTAAGGA+AGG 0.507670 3.4:-22199920 MS.gene82292:CDS
CGAAGCATGTGTGTAGTCTC+TGG 0.507970 3.4:+22199740 None:intergenic
TGACATGCTAAAGAAAATGA+TGG 0.509229 3.4:-22198214 MS.gene82292:CDS
TTGGAAGCCAAAGGTTGGAA+TGG 0.512541 3.4:-22200059 MS.gene82292:CDS
TTGAATCCCTTTGCTTGCAT+AGG 0.513123 3.4:+22198123 None:intergenic
CCAATATTTGGATCGTCAAA+GGG 0.514929 3.4:+22197692 None:intergenic
GTTGATTGGAAGCCAAAGGT+TGG 0.521153 3.4:-22200064 MS.gene82292:CDS
GTTCGTTTGTTGTAAGGAAG+GGG 0.523718 3.4:-22199918 MS.gene82292:CDS
TATGTTCATCTAGAAACTCA+TGG 0.526110 3.4:+22199780 None:intergenic
ATGGATAAGAACGGTGAAAA+CGG 0.526574 3.4:-22198174 MS.gene82292:CDS
ACACTTTCACTTTGTAGGAA+CGG 0.535912 3.4:-22199815 MS.gene82292:CDS
CAACTTATGAGCACACATGC+AGG 0.539062 3.4:-22199635 MS.gene82292:CDS
TTTATGGGAAATGAGGTTGA+AGG 0.539484 3.4:-22198354 MS.gene82292:CDS
ACTTTGTAGGAACGGAAAGT+TGG 0.539900 3.4:-22199807 MS.gene82292:CDS
CTTCCTACAACTACAAGCAA+TGG 0.547623 3.4:+22198506 None:intergenic
TTCTGATCCTATGCAAGCAA+AGG 0.549891 3.4:-22198130 MS.gene82292:CDS
AATCCTCACATAAGCTTGCA+AGG 0.551010 3.4:-22198734 MS.gene82292:CDS
CTGATATGTATATCTTAAGA+AGG 0.552468 3.4:-22198413 MS.gene82292:CDS
GCTTGCTTATGGTTTACGTG+TGG 0.553841 3.4:-22200002 MS.gene82292:CDS
AAATGTTGATTGGAAGCCAA+AGG 0.556734 3.4:-22200068 MS.gene82292:CDS
CACTGTTATCAACAAACTTG+AGG 0.562499 3.4:+22198241 None:intergenic
TCTGATCCTATGCAAGCAAA+GGG 0.565167 3.4:-22198129 MS.gene82292:CDS
CTCTAAGTTCATTAGAGCAG+CGG 0.565189 3.4:-22198289 MS.gene82292:CDS
TTGCCAATATGTCATCACTA+AGG 0.566698 3.4:-22198577 MS.gene82292:CDS
ACCTAAACAAAGAAGTGCAC+AGG 0.577644 3.4:-22198016 MS.gene82292:intron
GTGAAAGTGTAATTCTTGCA+AGG 0.578211 3.4:+22199828 None:intergenic
ATGAAGGAAGTGTTAACACT+TGG 0.582316 3.4:-22198900 MS.gene82292:CDS
CAACCTTGCAAGCTTATGTG+AGG 0.584735 3.4:+22198731 None:intergenic
ATATCTTAAGAAGGTGGACA+AGG 0.586063 3.4:-22198404 MS.gene82292:CDS
AACTTATGAGCACACATGCA+GGG 0.592671 3.4:-22199634 MS.gene82292:CDS
TTAACAGCTCATATGGTCGA+TGG 0.594285 3.4:-22197880 MS.gene82292:CDS
GTCTTGTATGCAACCAAATG+TGG 0.594828 3.4:-22198832 MS.gene82292:CDS
AGGAAGGGGTGAAGAGTACA+GGG 0.606717 3.4:-22199904 MS.gene82292:CDS
ATATGTATATCTTAAGAAGG+TGG 0.611193 3.4:-22198410 MS.gene82292:CDS
GCAGGGCATAGAGCTAATGT+AGG 0.612172 3.4:-22199617 MS.gene82292:CDS
ATGGGCGGCGTGTTACATGA+AGG 0.617720 3.4:-22198916 MS.gene82292:CDS
ACGGCACTGTATCATCATGT+AGG 0.621980 3.4:-22199940 MS.gene82292:CDS
ATGTGAAATTACCATTAACA+TGG 0.624421 3.4:+22197789 None:intergenic
TTTACAAACAGGAAGAAAGA+CGG 0.640619 3.4:-22199959 MS.gene82292:CDS
TTCTTTGTTTAGGTGGAGCG+CGG 0.641114 3.4:+22198025 None:intergenic
TAATGTAGGATTTACAGAGA+AGG 0.645234 3.4:-22199603 MS.gene82292:CDS
TGTGCTCATAAGTTGAAATG+TGG 0.654667 3.4:+22199643 None:intergenic
AAGGGATTCAAGAATCGACC+CGG 0.658744 3.4:-22198111 MS.gene82292:CDS
TATCTTAAGAAGGTGGACAA+GGG 0.672366 3.4:-22198403 MS.gene82292:CDS
GCACAAGCTATGGATAAGAA+CGG 0.681477 3.4:-22198183 MS.gene82292:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACATAATTATTTTAGGTTAA+AGG - chr3.4:22199845-22199864 MS.gene82292:CDS 15.0%
!! ATAATATTCGTGATAAATCT+TGG - chr3.4:22198781-22198800 MS.gene82292:CDS 20.0%
!! CATATTATTAACACAAAACT+GGG + chr3.4:22199789-22199808 None:intergenic 20.0%
!! TGAAAAAAGAAAAAGAAGTA+TGG - chr3.4:22198188-22198207 MS.gene82292:CDS 20.0%
!! TTATGGGCTTAAAAAAAAAT+GGG - chr3.4:22198815-22198834 MS.gene82292:CDS 20.0%
!!! AACAAATTACAAATGTGTTT+TGG - chr3.4:22198304-22198323 MS.gene82292:CDS 20.0%
! ACAAGCATATGAAATTGATA+TGG - chr3.4:22198056-22198075 MS.gene82292:CDS 25.0%
! AGACAATTGAGTCATTTAAA+TGG - chr3.4:22198487-22198506 MS.gene82292:CDS 25.0%
! ATATGTATATCTTAAGAAGG+TGG - chr3.4:22199339-22199358 MS.gene82292:intron 25.0%
! ATGTGAAATTACCATTAACA+TGG + chr3.4:22199963-22199982 None:intergenic 25.0%
! ATTCGATTTCTTAACAACTT+AGG + chr3.4:22199425-22199444 None:intergenic 25.0%
! CCATATTATTAACACAAAAC+TGG + chr3.4:22199790-22199809 None:intergenic 25.0%
! CTGATATGTATATCTTAAGA+AGG - chr3.4:22199336-22199355 MS.gene82292:intron 25.0%
! CTTATGGGCTTAAAAAAAAA+TGG - chr3.4:22198814-22198833 MS.gene82292:CDS 25.0%
! CTTCTTAAGATATACATATC+AGG + chr3.4:22199338-22199357 None:intergenic 25.0%
! CTTTGAATTGAATGATGTTA+AGG - chr3.4:22199307-22199326 MS.gene82292:intron 25.0%
! TAAGAATATGCTCCAATATT+TGG + chr3.4:22200072-22200091 None:intergenic 25.0%
!! ATATTTTCAACGACAATTGT+TGG - chr3.4:22198239-22198258 MS.gene82292:CDS 25.0%
!! TCATGATACTTTTACATGTA+AGG + chr3.4:22200005-22200024 None:intergenic 25.0%
!!! CACATTTGTAATTTGTTCTT+CGG + chr3.4:22198301-22198320 None:intergenic 25.0%
!!! CCAGTTTTGTGTTAATAATA+TGG - chr3.4:22199787-22199806 MS.gene82292:CDS 25.0%
!!! TATCTCTTTGTTATTAACGT+AGG - chr3.4:22200032-22200051 MS.gene82292:CDS 25.0%
AAAAAAAGCAAGTGGTCTAT+TGG + chr3.4:22198409-22198428 None:intergenic 30.0%
AAAATGGAGTCAAACATCTT+GGG - chr3.4:22198649-22198668 MS.gene82292:CDS 30.0%
AAACCAGAAAAAAAAGCAAG+TGG + chr3.4:22198417-22198436 None:intergenic 30.0%
ATCACTAAGGAAAAACATGA+AGG - chr3.4:22199185-22199204 MS.gene82292:intron 30.0%
CGAAATGATTGAAGAATTCT+AGG + chr3.4:22198947-22198966 None:intergenic 30.0%
GAATTACACTTTCACTTTGT+AGG - chr3.4:22197929-22197948 MS.gene82292:intron 30.0%
TAAAGGATTAACAGCTCATA+TGG - chr3.4:22199862-22199881 MS.gene82292:CDS 30.0%
TAATGTAGGATTTACAGAGA+AGG - chr3.4:22198146-22198165 MS.gene82292:CDS 30.0%
TATGTTCATCTAGAAACTCA+TGG + chr3.4:22197972-22197991 None:intergenic 30.0%
TGACATGCTAAAGAAAATGA+TGG - chr3.4:22199535-22199554 MS.gene82292:intron 30.0%
TTTACAAACAGGAAGAAAGA+CGG - chr3.4:22197790-22197809 MS.gene82292:intron 30.0%
TTTCCTTAGTGATGACATAT+TGG + chr3.4:22199178-22199197 None:intergenic 30.0%
! ATTTTGGATTACGGGTATTT+TGG - chr3.4:22198342-22198361 MS.gene82292:CDS 30.0%
! GGGATTTGATAATATGGAAA+AGG - chr3.4:22197711-22197730 MS.gene82292:intron 30.0%
! TGGAAAAGGCTAAACAATTT+TGG - chr3.4:22197725-22197744 MS.gene82292:intron 30.0%
! TGGAATGGGATTTGATAATA+TGG - chr3.4:22197705-22197724 MS.gene82292:intron 30.0%
! TGTGTATACTTTTCCCATTT+GGG + chr3.4:22199069-22199088 None:intergenic 30.0%
! TTAGAGTACGTTTTACAAAC+AGG - chr3.4:22197779-22197798 MS.gene82292:intron 30.0%
!! AATTCTAGGATATCCACATT+TGG + chr3.4:22198933-22198952 None:intergenic 30.0%
!! CATCAGATTTTGAGAAACTT+TGG - chr3.4:22198739-22198758 MS.gene82292:CDS 30.0%
!!! AAACAATTTTGGCTTGCTTA+TGG - chr3.4:22197736-22197755 MS.gene82292:intron 30.0%
!!! TCTGGTTTTAACCATTATAG+AGG - chr3.4:22198429-22198448 MS.gene82292:CDS 30.0%
AAAGGATCGAACCTCTATAA+TGG + chr3.4:22198443-22198462 None:intergenic 35.0%
AATCTTGCACAATGCCATAA+CGG + chr3.4:22199376-22199395 None:intergenic 35.0%
ACACTTTCACTTTGTAGGAA+CGG - chr3.4:22197934-22197953 MS.gene82292:intron 35.0%
AGGAAAAACATGAAGGATCT+TGG - chr3.4:22199192-22199211 MS.gene82292:intron 35.0%
ATATCTTAAGAAGGTGGACA+AGG - chr3.4:22199345-22199364 MS.gene82292:intron 35.0%
ATGGATAAGAACGGTGAAAA+CGG - chr3.4:22199575-22199594 MS.gene82292:CDS 35.0%
ATTGATATGGCCAATAACTC+TGG - chr3.4:22198069-22198088 MS.gene82292:CDS 35.0%
CAAAATGGAGTCAAACATCT+TGG - chr3.4:22198648-22198667 MS.gene82292:CDS 35.0%
CACTGTTATCAACAAACTTG+AGG + chr3.4:22199511-22199530 None:intergenic 35.0%
CATCTGATATGCATGAAAGA+AGG + chr3.4:22198274-22198293 None:intergenic 35.0%
CCAATATTTGGATCGTCAAA+GGG + chr3.4:22200060-22200079 None:intergenic 35.0%
CTGTCTTAATCCAGAGTTAT+TGG + chr3.4:22198082-22198101 None:intergenic 35.0%
CTTCTTTCATGCATATCAGA+TGG - chr3.4:22198272-22198291 MS.gene82292:CDS 35.0%
CTTGGATCAAGACAACTTAT+GGG - chr3.4:22198799-22198818 MS.gene82292:CDS 35.0%
GAGTCAGTTTCATGTCTAAT+TGG + chr3.4:22199149-22199168 None:intergenic 35.0%
GGGAACAAAAATGCATATGA+AGG - chr3.4:22197865-22197884 MS.gene82292:CDS 35.0%
TAACTACACAGCCATGTTAA+TGG - chr3.4:22199949-22199968 MS.gene82292:CDS 35.0%
TATCTTAAGAAGGTGGACAA+GGG - chr3.4:22199346-22199365 MS.gene82292:intron 35.0%
TCACCAATACTAACCCAAAT+GGG - chr3.4:22199053-22199072 MS.gene82292:intron 35.0%
TCCAATATTTGGATCGTCAA+AGG + chr3.4:22200061-22200080 None:intergenic 35.0%
TCTTGGATCAAGACAACTTA+TGG - chr3.4:22198798-22198817 MS.gene82292:CDS 35.0%
TGATATGCATGAAAGAAGGA+TGG + chr3.4:22198270-22198289 None:intergenic 35.0%
TGGCATCTACAAATGTTGAT+TGG - chr3.4:22197671-22197690 MS.gene82292:CDS 35.0%
TGGGCTTAAAAAAAAATGGG+CGG - chr3.4:22198818-22198837 MS.gene82292:CDS 35.0%
TTCACCAATACTAACCCAAA+TGG - chr3.4:22199052-22199071 MS.gene82292:intron 35.0%
TTGCCAATATGTCATCACTA+AGG - chr3.4:22199172-22199191 MS.gene82292:intron 35.0%
TTTATGGGAAATGAGGTTGA+AGG - chr3.4:22199395-22199414 MS.gene82292:intron 35.0%
! AAATGTTGATTGGAAGCCAA+AGG - chr3.4:22197681-22197700 MS.gene82292:CDS 35.0%
! ACATGGCACTTGTTACAAAA+TGG - chr3.4:22198633-22198652 MS.gene82292:CDS 35.0%
! ACTTGATTTTCTTGTGCTTG+TGG + chr3.4:22199929-22199948 None:intergenic 35.0%
! AGAAAAAGAAGTATGGCGTA+TGG - chr3.4:22198195-22198214 MS.gene82292:CDS 35.0%
! CACGTATGATTTTGGATTAC+GGG - chr3.4:22198334-22198353 MS.gene82292:CDS 35.0%
! CTTGATTTTCTTGTGCTTGT+GGG + chr3.4:22199928-22199947 None:intergenic 35.0%
! GCAAGATTTTATGGGAAATG+AGG - chr3.4:22199388-22199407 MS.gene82292:intron 35.0%
! GCACGTATGATTTTGGATTA+CGG - chr3.4:22198333-22198352 MS.gene82292:CDS 35.0%
! GGCATTGTGCAAGATTTTAT+GGG - chr3.4:22199380-22199399 MS.gene82292:intron 35.0%
! GTCTAGTAAGGTGTTTATGT+TGG - chr3.4:22199763-22199782 MS.gene82292:CDS 35.0%
! GTGAAAGTGTAATTCTTGCA+AGG + chr3.4:22197924-22197943 None:intergenic 35.0%
! GTGTGTATACTTTTCCCATT+TGG + chr3.4:22199070-22199089 None:intergenic 35.0%
! TATAGAGGTTCGATCCTTTT+TGG - chr3.4:22198444-22198463 MS.gene82292:CDS 35.0%
! TGGCATTGTGCAAGATTTTA+TGG - chr3.4:22199379-22199398 MS.gene82292:intron 35.0%
! TGTAGGAAGTTTGAGACATT+TGG - chr3.4:22199257-22199276 MS.gene82292:intron 35.0%
! TGTGCTCATAAGTTGAAATG+TGG + chr3.4:22198109-22198128 None:intergenic 35.0%
! TTGATTTTCTTGTGCTTGTG+GGG + chr3.4:22199927-22199946 None:intergenic 35.0%
!! ATGAAGGAAGTGTTAACACT+TGG - chr3.4:22198849-22198868 MS.gene82292:CDS 35.0%
!! ATGTAGGTTCGTTTGTTGTA+AGG - chr3.4:22197825-22197844 MS.gene82292:CDS 35.0%
!! TTTCCCATTTGGGTTAGTAT+TGG + chr3.4:22199059-22199078 None:intergenic 35.0%
!!! AGACCACTTGCTTTTTTTTC+TGG - chr3.4:22198411-22198430 MS.gene82292:CDS 35.0%
AACTTATGAGCACACATGCA+GGG - chr3.4:22198115-22198134 MS.gene82292:CDS 40.0%
AATCCTCACATAAGCTTGCA+AGG - chr3.4:22199015-22199034 MS.gene82292:intron 40.0%
ACATCTTGGGAACTTGATGA+AGG - chr3.4:22198662-22198681 MS.gene82292:CDS 40.0%
ACATTAGTGCTGCACCAAAA+AGG + chr3.4:22198461-22198480 None:intergenic 40.0%
ACTTTGTAGGAACGGAAAGT+TGG - chr3.4:22197942-22197961 MS.gene82292:intron 40.0%
ATCTTGCACAATGCCATAAC+GGG + chr3.4:22199375-22199394 None:intergenic 40.0%
CACTGACCAAGATCAATCAA+TGG - chr3.4:22198563-22198582 MS.gene82292:CDS 40.0%
CATCTTGGGAACTTGATGAA+GGG - chr3.4:22198663-22198682 MS.gene82292:CDS 40.0%
CCCTTTGACGATCCAAATAT+TGG - chr3.4:22200057-22200076 MS.gene82292:CDS 40.0%
CTCTAAGTTCATTAGAGCAG+CGG - chr3.4:22199460-22199479 MS.gene82292:intron 40.0%
CTTCCATTGCTTGTAGTTGT+AGG - chr3.4:22199240-22199259 MS.gene82292:intron 40.0%
CTTCCTACAACTACAAGCAA+TGG + chr3.4:22199246-22199265 None:intergenic 40.0%
CTTGCTTATGGTTTACGTGT+GGG - chr3.4:22197748-22197767 MS.gene82292:intron 40.0%
GCACAAGCTATGGATAAGAA+CGG - chr3.4:22199566-22199585 MS.gene82292:CDS 40.0%
GGATCAGAACTCATAATTGC+CGG + chr3.4:22199608-22199627 None:intergenic 40.0%
GTCTTGTATGCAACCAAATG+TGG - chr3.4:22198917-22198936 MS.gene82292:CDS 40.0%
TCAAATCCCATTCCAACCTT+TGG + chr3.4:22197700-22197719 None:intergenic 40.0%
TCTGATCCTATGCAAGCAAA+GGG - chr3.4:22199620-22199639 MS.gene82292:CDS 40.0%
TTAACAGCTCATATGGTCGA+TGG - chr3.4:22199869-22199888 MS.gene82292:CDS 40.0%
TTCTGATCCTATGCAAGCAA+AGG - chr3.4:22199619-22199638 MS.gene82292:CDS 40.0%
TTGAATCCCTTTGCTTGCAT+AGG + chr3.4:22199629-22199648 None:intergenic 40.0%
! AACAAAGAAGTGCACAGGTA+TGG - chr3.4:22199738-22199757 MS.gene82292:CDS 40.0%
! ACCTAAACAAAGAAGTGCAC+AGG - chr3.4:22199733-22199752 MS.gene82292:CDS 40.0%
! ACCTGTGCACTTCTTTGTTT+AGG + chr3.4:22199737-22199756 None:intergenic 40.0%
! CACTTGCCATTGATTGATCT+TGG + chr3.4:22198572-22198591 None:intergenic 40.0%
! GTGTAGTCTCTGGATTTTGT+AGG + chr3.4:22198002-22198021 None:intergenic 40.0%
! GTGTGATGCACGTATGATTT+TGG - chr3.4:22198326-22198345 MS.gene82292:CDS 40.0%
! TCTCTGGATTTTGTAGGTCA+TGG + chr3.4:22197996-22198015 None:intergenic 40.0%
! TGATTTTCTTGTGCTTGTGG+GGG + chr3.4:22199926-22199945 None:intergenic 40.0%
! TGTGCACTTCTTTGTTTAGG+TGG + chr3.4:22199734-22199753 None:intergenic 40.0%
!! AAGGCAACTTTTAAGTCGCT+TGG + chr3.4:22199675-22199694 None:intergenic 40.0%
!! AGGTTCGTTTGTTGTAAGGA+AGG - chr3.4:22197829-22197848 MS.gene82292:CDS 40.0%
!! GGTTCGTTTGTTGTAAGGAA+GGG - chr3.4:22197830-22197849 MS.gene82292:CDS 40.0%
!! GTTCGTTTGTTGTAAGGAAG+GGG - chr3.4:22197831-22197850 MS.gene82292:CDS 40.0%
!!! CTTGACTATGCTGCTTTTCA+AGG + chr3.4:22199694-22199713 None:intergenic 40.0%
AAGCAAGTGGTCTATTGGCA+TGG + chr3.4:22198404-22198423 None:intergenic 45.0%
AAGGGATTCAAGAATCGACC+CGG - chr3.4:22199638-22199657 MS.gene82292:CDS 45.0%
AAGTGGTCTATTGGCATGGT+TGG + chr3.4:22198400-22198419 None:intergenic 45.0%
ACAGGTATGGCTGTCTAGTA+AGG - chr3.4:22199751-22199770 MS.gene82292:CDS 45.0%
ACGGCACTGTATCATCATGT+AGG - chr3.4:22197809-22197828 MS.gene82292:CDS 45.0%
AGTATGGCGTATGGTGAGAT+TGG - chr3.4:22198204-22198223 MS.gene82292:CDS 45.0%
AGTGCCATGTGCATAAACCA+TGG + chr3.4:22198623-22198642 None:intergenic 45.0%
CAACCTTGCAAGCTTATGTG+AGG + chr3.4:22199021-22199040 None:intergenic 45.0%
CAACTTATGAGCACACATGC+AGG - chr3.4:22198114-22198133 MS.gene82292:CDS 45.0%
CATAAACCATGGTGAGTCTC+AGG + chr3.4:22198612-22198631 None:intergenic 45.0%
CTCACCATGGTTTATGCACA+TGG - chr3.4:22198616-22198635 MS.gene82292:CDS 45.0%
GCTTGCTTATGGTTTACGTG+TGG - chr3.4:22197747-22197766 MS.gene82292:intron 45.0%
GTTGATTGGAAGCCAAAGGT+TGG - chr3.4:22197685-22197704 MS.gene82292:CDS 45.0%
TATGGTTTACGTGTGGGCTT+CGG - chr3.4:22197754-22197773 MS.gene82292:intron 45.0%
TGGAAGCCAAAGGTTGGAAT+GGG - chr3.4:22197691-22197710 MS.gene82292:CDS 45.0%
TGGTCAGTGAAGATCGTCTT+TGG + chr3.4:22198552-22198571 None:intergenic 45.0%
TTGGAAGCCAAAGGTTGGAA+TGG - chr3.4:22197690-22197709 MS.gene82292:CDS 45.0%
! TTCTTTGTTTAGGTGGAGCG+CGG + chr3.4:22199727-22199746 None:intergenic 45.0%
!! TCGCTTGGTTCTTTTTGAGC+CGG + chr3.4:22199660-22199679 None:intergenic 45.0%
!!! TAATTTTACATAATTATTTT+AGG - chr3.4:22199838-22199857 MS.gene82292:CDS 5.0%
CGAAGCATGTGTGTAGTCTC+TGG + chr3.4:22198012-22198031 None:intergenic 50.0%
CTTGGGAACTTGATGAAGGG+AGG - chr3.4:22198666-22198685 MS.gene82292:CDS 50.0%
GCAGGGCATAGAGCTAATGT+AGG - chr3.4:22198132-22198151 MS.gene82292:CDS 50.0%
! AAGGAAGGGGTGAAGAGTAC+AGG - chr3.4:22197844-22197863 MS.gene82292:CDS 50.0%
! AGGAAGGGGTGAAGAGTACA+GGG - chr3.4:22197845-22197864 MS.gene82292:CDS 50.0%
! GGCATGGTTGGTGCAATATG+TGG + chr3.4:22198388-22198407 None:intergenic 50.0%
! TGTGGGGGCACATAAGTTGA+AGG + chr3.4:22199911-22199930 None:intergenic 50.0%
!! CGCTTGGTTCTTTTTGAGCC+GGG + chr3.4:22199659-22199678 None:intergenic 50.0%
ATGGGCGGCGTGTTACATGA+AGG - chr3.4:22198833-22198852 MS.gene82292:CDS 55.0%
GGAATCTCGCGCACAAGCTA+TGG - chr3.4:22199556-22199575 MS.gene82292:CDS 55.0%
GTGATGCCTGAGACTCACCA+TGG - chr3.4:22198603-22198622 MS.gene82292:CDS 55.0%
TGGACAAGGGAAGCCCGTTA+TGG - chr3.4:22199359-22199378 MS.gene82292:intron 55.0%
CGGTGAAAACGGTGCTCGTC+CGG - chr3.4:22199586-22199605 MS.gene82292:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 22197670 22200101 22197670 ID=MS.gene82292
chr3.4 mRNA 22197670 22200101 22197670 ID=MS.gene82292.t1;Parent=MS.gene82292
chr3.4 exon 22199555 22200101 22199555 ID=MS.gene82292.t1.exon1;Parent=MS.gene82292.t1
chr3.4 CDS 22199555 22200101 22199555 ID=cds.MS.gene82292.t1;Parent=MS.gene82292.t1
chr3.4 exon 22198017 22199026 22198017 ID=MS.gene82292.t1.exon2;Parent=MS.gene82292.t1
chr3.4 CDS 22198017 22199026 22198017 ID=cds.MS.gene82292.t1;Parent=MS.gene82292.t1
chr3.4 exon 22197801 22197911 22197801 ID=MS.gene82292.t1.exon3;Parent=MS.gene82292.t1
chr3.4 CDS 22197801 22197911 22197801 ID=cds.MS.gene82292.t1;Parent=MS.gene82292.t1
chr3.4 exon 22197670 22197717 22197670 ID=MS.gene82292.t1.exon4;Parent=MS.gene82292.t1
chr3.4 CDS 22197670 22197717 22197670 ID=cds.MS.gene82292.t1;Parent=MS.gene82292.t1
Gene Sequence

>MS.gene82292

ATGGCATCTACAAATGTTGATTGGAAGCCAAAGGTTGGAATGGGATTTGATAATATGGAAAAGGCTAAACAATTTTGGCTTGCTTATGGTTTACGTGTGGGCTTCGGAATTAGAGTACGTTTTACAAACAGGAAGAAAGACGGCACTGTATCATCATGTAGGTTCGTTTGTTGTAAGGAAGGGGTGAAGAGTACAGGGAACAAAAATGCATATGAAGGAAAATATGAGAGAGCTGATATTAGAACAAATTGCCTTGCAAGAATTACACTTTCACTTTGTAGGAACGGAAAGTTGGTCATCCATGAGTTTCTAGATGAACATAACCATGACCTACAAAATCCAGAGACTACACACATGCTTCGATCACATAGAAAGATAACTGAAGTACAAGCATATGAAATTGATATGGCCAATAACTCTGGATTAAGACAGAAGTCCACATTTCAACTTATGAGCACACATGCAGGGCATAGAGCTAATGTAGGATTTACAGAGAAGGATGTAAGAAACTACATCACTGAAAAAAGAAAAAGAAGTATGGCGTATGGTGAGATTGGATGTCTTTCACAATATTTTCAACGACAATTGTTGGAGAATCCATCCTTCTTTCATGCATATCAGATGGATGCCGAAGAACAAATTACAAATGTGTTTTGGTGTGATGCACGTATGATTTTGGATTACGGGTATTTTGGTGATGTTGTTTCATTAGACACCACATATTGCACCAACCATGCCAATAGACCACTTGCTTTTTTTTCTGGTTTTAACCATTATAGAGGTTCGATCCTTTTTGGTGCAGCACTAATGTATGATGAGACAATTGAGTCATTTAAATGGTTGTTTGATACATTTTTACAAGCACACAACAACAAAAAACCAAAGACGATCTTCACTGACCAAGATCAATCAATGGCAAGTGCACTTGAAAAAGTGATGCCTGAGACTCACCATGGTTTATGCACATGGCACTTGTTACAAAATGGAGTCAAACATCTTGGGAACTTGATGAAGGGAGGAAGTTTTCTGCTTAGAGATATAAAAAAATGCATGTATGATATTGACAATGCATCAGATTTTGAGAAACTTTGGTTTGATTTGATCAACGAATATAATATTCGTGATAAATCTTGGATCAAGACAACTTATGGGCTTAAAAAAAAATGGGCGGCGTGTTACATGAAGGAAGTGTTAACACTTGGCATGCGAAGTACACAACTTAGTGAAAGTTTGAATGCTCACTTCAAGTCTTGTATGCAACCAAATGTGGATATCCTAGAATTCTTCAATCATTTCGAAAGAGTTGTTGATGAGAAGAGAGCAAAAGAATTGAGTTGTGTGTATGAATCCTCACATAAGCTTGCAAGGTTGAAATATGAAACTTCACCAATACTAACCCAAATGGGAAAAGTATACACACACACTGTCTTTGAATTGTTTCAAGATGAGTTTAAGTCATTATTAGCTCTATCTGTACCAATTAGACATGAAACTGACTCTCTTTGCCAATATGTCATCACTAAGGAAAAACATGAAGGATCTTGGAGAGTGTCATTTAATCGTGTCTCAACTTCCATTGCTTGTAGTTGTAGGAAGTTTGAGACATTTGGCATACTTTGTTCACATGCATTAAAGATCTTTGAATTGAATGATGTTAAGGTAATGCCTGATATGTATATCTTAAGAAGGTGGACAAGGGAAGCCCGTTATGGCATTGTGCAAGATTTTATGGGAAATGAGGTTGAAGGAGATCCTAAGTTGTTAAGAAATCGAATGTACAGACAAGTTGTCTCTAAGTTCATTAGAGCAGCGGCTGAAGCATCAAATGATGAAGAAAGCCTCAAGTTTGTTGATAACAGTGTAGATGACATGCTAAAGAAAATGATGGAATCTCGCGCACAAGCTATGGATAAGAACGGTGAAAACGGTGCTCGTCCGGCAATTATGAGTTCTGATCCTATGCAAGCAAAGGGATTCAAGAATCGACCCGGCTCAAAAAGAACCAAGCGACTTAAAAGTTGCCTTGAAAAGCAGCATAGTCAAGCTACATCTAGCCGCGCTCCACCTAAACAAAGAAGTGCACAGGTATGGCTGTCTAGTAAGGTGTTTATGTTGGCCCAGTTTTGTGTTAATAATATGGCTAAAGAATTAATAAAATTAGCTTATTTTAATTTTACATAATTATTTTAGGTTAAAGGATTAACAGCTCATATGGTCGATGGAATATCTTGTTCTGCGCCTTCAACTTATGTGCCCCCACAAGCACAAGAAAATCAAGTTAACTACACAGCCATGTTAATGGTAATTTCACATCTTTTTCATTATGAAATATCCTTACATGTAAAAGTATCATGATACTAATTATCTCTTTGTTATTAACGTAGGCACCCTTTGACGATCCAAATATTGGAGCATATTCTTACCTTGATTGA
Protein sequence

>MS.gene82292.t1

MASTNVDWKPKVGMGFDNMEKAKQFWLAYGLRVGFGIRVRFTNRKKDGTVSSCRFVCCKEGVKSTGNKNAYEGKYERADIRTNCLARITLSLCRNGKLVIHEFLDEHNHDLQNPETTHMLRSHRKITEVQAYEIDMANNSGLRQKSTFQLMSTHAGHRANVGFTEKDVRNYITEKRKRSMAYDFEKLWFDLINEYNIRDKSWIKTTYGLKKKWAACYMKEVLTLGMRSTQLSESLNAHFKSCMQPNVDILEFFNHFERVVDEKRAKELSCVYESSHKLARLKYETSPILTQMGKVYTHTVFELFQDEFKSLLALSVPIRHETDSLCQYVITKEKHEGSWRVSFNRVSTSIACSCRKFETFGILCSHALKIFELNDVKVMPDMYILRRWTREARYGIVQDFMGNEVEGDPKLLRNRMYRQVVSKFIRAAAEASNDEESLKFVDNSVDDMLKKMMESRAQAMDKNGENGARPAIMSSDPMQAKGFKNRPGSKRTKRLKSCLEKQHSQATSSRAPPKQRSAQVKGLTAHMVDGISCSAPSTYVPPQAQENQVNYTAMLMAPFDDPNIGAYSYLD