Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82296.t1 | XP_003599125.1 | 98 | 410 | 7 | 1 | 1 | 409 | 1 | 410 | 2.10E-217 | 765 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82296.t1 | E0X9N4 | 54.9 | 319 | 129 | 7 | 1 | 307 | 1 | 316 | 1.9e-73 | 278.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82296.t1 | G7IWL7 | 98.0 | 410 | 7 | 1 | 1 | 409 | 1 | 410 | 1.5e-217 | 765.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene82296.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82296.t1 | MTR_3g028170 | 98.380 | 432 | 4 | 2 | 1 | 431 | 1 | 430 | 0.0 | 885 |
| MS.gene82296.t1 | MTR_3g028190 | 52.888 | 329 | 126 | 8 | 1 | 307 | 1 | 322 | 1.48e-106 | 321 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82296.t1 | AT3G08505 | 51.125 | 311 | 125 | 8 | 1 | 307 | 1 | 288 | 7.27e-100 | 303 |
| MS.gene82296.t1 | AT3G08505 | 51.125 | 311 | 125 | 8 | 1 | 307 | 1 | 288 | 7.27e-100 | 303 |
| MS.gene82296.t1 | AT3G08505 | 51.125 | 311 | 125 | 8 | 1 | 307 | 1 | 288 | 7.27e-100 | 303 |
Find 109 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGCATCCTACTGATAAAA+AGG | 0.246259 | 3.4:+22061123 | MS.gene82296:CDS |
| CAGAAAGGGTCTTGTGCTTA+TGG | 0.259648 | 3.4:+22060779 | MS.gene82296:CDS |
| GATTCGCTGGCTGGCGGTTC+AGG | 0.263297 | 3.4:+22069591 | MS.gene82296:CDS |
| GATGCAGCAACACCGCTATT+TGG | 0.292037 | 3.4:+22070738 | MS.gene82296:intron |
| AGCATCATCTTCAGCATCTA+TGG | 0.292528 | 3.4:+22060838 | MS.gene82296:CDS |
| GTGCTAAAGTAGCATCAAAT+TGG | 0.294959 | 3.4:+22060891 | MS.gene82296:CDS |
| TTTGAGGACTTGTGACAAAA+AGG | 0.300862 | 3.4:+22061159 | MS.gene82296:CDS |
| GCTCTTGGGATGGATGCTTT+TGG | 0.301364 | 3.4:+22069696 | MS.gene82296:CDS |
| TTGTCATTCCAAGTGGTATT+TGG | 0.301592 | 3.4:+22061422 | MS.gene82296:CDS |
| AAGCCATCAGAATGTAAGTT+TGG | 0.301620 | 3.4:+22061259 | MS.gene82296:CDS |
| TGGCGGTTCAGGTCCTTGTT+TGG | 0.314287 | 3.4:+22069602 | MS.gene82296:CDS |
| GGCGGTTCAGGTCCTTGTTT+GGG | 0.319796 | 3.4:+22069603 | MS.gene82296:CDS |
| GGAAATGGAAATTGTCCATT+TGG | 0.321258 | 3.4:+22061629 | MS.gene82296:CDS |
| TCCTCATCGTCATCGTCATC+AGG | 0.328553 | 3.4:-22069720 | None:intergenic |
| ATGCAAGAGGCCATGGCTCT+TGG | 0.356930 | 3.4:+22069681 | MS.gene82296:CDS |
| CAGAAAGCATGATCACATTC+TGG | 0.360354 | 3.4:-22061289 | None:intergenic |
| TATGATAGTTTGCGGCAAAC+AGG | 0.366308 | 3.4:-22061397 | None:intergenic |
| GAAATGGAAATTGTCCATTT+GGG | 0.373127 | 3.4:+22061630 | MS.gene82296:CDS |
| CGATTTCCATTGCAGAGGTT+GGG | 0.374375 | 3.4:-22061345 | None:intergenic |
| CCCTTTACGCCACGCTTGTC+AGG | 0.376758 | 3.4:-22060941 | None:intergenic |
| CCTGACAAGCGTGGCGTAAA+GGG | 0.379956 | 3.4:+22060941 | MS.gene82296:CDS |
| GTTTGTCATGTATCCGCAAT+TGG | 0.381206 | 3.4:+22061311 | MS.gene82296:CDS |
| AAACTTACATTCTGATGGCT+TGG | 0.395562 | 3.4:-22061257 | None:intergenic |
| AACTTACATTCTGATGGCTT+GGG | 0.410250 | 3.4:-22061256 | None:intergenic |
| TGCAAGAGGCCATGGCTCTT+GGG | 0.417374 | 3.4:+22069682 | MS.gene82296:CDS |
| ACCTGACAAGCGTGGCGTAA+AGG | 0.437739 | 3.4:+22060940 | MS.gene82296:CDS |
| GATAATGCCATCATCTCAAA+TGG | 0.443626 | 3.4:-22069567 | None:intergenic |
| AAAATCTCACCATGTCCAAC+AGG | 0.444234 | 3.4:+22059781 | None:intergenic |
| AGCCATCAGAATGTAAGTTT+GGG | 0.447348 | 3.4:+22061260 | MS.gene82296:CDS |
| CCGATTTCCATTGCAGAGGT+TGG | 0.450187 | 3.4:-22061346 | None:intergenic |
| GATTCAGACGATGAGGATGA+TGG | 0.450779 | 3.4:+22069633 | MS.gene82296:CDS |
| CCAACCTCTGCAATGGAAAT+CGG | 0.452415 | 3.4:+22061346 | MS.gene82296:CDS |
| TTTGCTCTTATTATCAGAAA+GGG | 0.457240 | 3.4:+22060765 | MS.gene82296:CDS |
| ACATGTCATGCATATCGAAA+TGG | 0.462288 | 3.4:-22069461 | None:intergenic |
| TGCTGCTATGCAAGAGGCCA+TGG | 0.469668 | 3.4:+22069674 | MS.gene82296:CDS |
| ATTTGCTCTTATTATCAGAA+AGG | 0.469932 | 3.4:+22060764 | MS.gene82296:CDS |
| GCTCGAGGCGTTTGTTTGAA+AGG | 0.470915 | 3.4:+22060439 | MS.gene82296:CDS |
| TTCCCTCCCTCCTCTTTCAA+CGG | 0.475235 | 3.4:-22070565 | None:intergenic |
| GGGTTTACAACGCAAGCATC+AGG | 0.478209 | 3.4:+22060961 | MS.gene82296:CDS |
| GCATTATCAGATTCGCTGGC+TGG | 0.489570 | 3.4:+22069582 | MS.gene82296:CDS |
| AATGGATCCATTTGAGATGA+TGG | 0.491467 | 3.4:+22069560 | MS.gene82296:CDS |
| GATTTCCATTGCAGAGGTTG+GGG | 0.493235 | 3.4:-22061344 | None:intergenic |
| TGCTAAAGTAGCATCAAATT+GGG | 0.494694 | 3.4:+22060892 | MS.gene82296:CDS |
| TAGAAACAGCTAGCCCCAAA+TGG | 0.494880 | 3.4:-22061644 | None:intergenic |
| GGCTGCTATGCAAGAGGCCA+TGG | 0.495488 | 3.4:+22069764 | MS.gene82296:CDS |
| TGGTCTAGCACTACCAGTAC+TGG | 0.497170 | 3.4:-22061005 | None:intergenic |
| TGTCTCCATGTGTATGAGCC+AGG | 0.499940 | 3.4:-22069408 | None:intergenic |
| TCCTCATCGTCTGAATCAAA+AGG | 0.507460 | 3.4:-22069627 | None:intergenic |
| GAAGGAACCTTTGTAACCTT+CGG | 0.509713 | 3.4:-22060917 | None:intergenic |
| AGGGCAGGTTCTCACTGTAT+TGG | 0.515799 | 3.4:-22061377 | None:intergenic |
| GATGGCATTATCAGATTCGC+TGG | 0.519486 | 3.4:+22069578 | MS.gene82296:CDS |
| TTTGCTGCTGCCAACTGCCC+CGG | 0.519590 | 3.4:+22061049 | MS.gene82296:CDS |
| TAGTTTGCGGCAAACAGGGC+AGG | 0.521329 | 3.4:-22061392 | None:intergenic |
| TGACAAAATATGATAGTTTG+CGG | 0.523339 | 3.4:-22061405 | None:intergenic |
| ACTTTAGCACTGCGAGCCAC+AGG | 0.525845 | 3.4:-22060878 | None:intergenic |
| TTCAGATTCTGCTGTGCCTG+TGG | 0.528627 | 3.4:+22060862 | MS.gene82296:CDS |
| TGAATACGAGAACATCCGCC+GGG | 0.529114 | 3.4:-22061067 | None:intergenic |
| AGAACATCCGCCGGGGCAGT+TGG | 0.529403 | 3.4:-22061059 | None:intergenic |
| AAAATCTGAGTCCCTCATGA+TGG | 0.533480 | 3.4:-22069529 | None:intergenic |
| TGGGGCTAGCTGTTTCTACA+AGG | 0.535393 | 3.4:+22061649 | MS.gene82296:CDS |
| ATTCAGACGATGAGGATGAT+GGG | 0.536703 | 3.4:+22069634 | MS.gene82296:CDS |
| GATTGTTTGACCGTTGAAAG+AGG | 0.538299 | 3.4:+22070555 | MS.gene82296:CDS |
| AACCCAAACTTACATTCTGA+TGG | 0.549424 | 3.4:-22061262 | None:intergenic |
| GTTTGACCGTTGAAAGAGGA+GGG | 0.552214 | 3.4:+22070559 | MS.gene82296:CDS |
| TTTCAGCATACAGTGAAGCC+TGG | 0.555178 | 3.4:+22069390 | MS.gene82296:intron |
| ATGATAGTTTGCGGCAAACA+GGG | 0.556192 | 3.4:-22061396 | None:intergenic |
| AACATGGATTTGAACTGTGA+TGG | 0.557338 | 3.4:-22070766 | None:intergenic |
| GTTGAAAGAGGAGGGAGGGA+AGG | 0.557464 | 3.4:+22070567 | MS.gene82296:CDS |
| TCAAATTGCTGCTATGCAAG+AGG | 0.561508 | 3.4:+22069668 | MS.gene82296:CDS |
| CATCAGGACTCTACCGATGT+TGG | 0.562367 | 3.4:+22060977 | MS.gene82296:CDS |
| GACCGTTGAAAGAGGAGGGA+GGG | 0.565159 | 3.4:+22070563 | MS.gene82296:CDS |
| TGGTATTTGGTTTACTACAA+AGG | 0.568402 | 3.4:+22061435 | MS.gene82296:CDS |
| GATGTTGGTGAATCCAGTAC+TGG | 0.570914 | 3.4:+22060992 | MS.gene82296:CDS |
| GGCGGATGTTCTCGTATTCA+CGG | 0.571733 | 3.4:+22061070 | MS.gene82296:CDS |
| AGCATCAAATTGGGTTCCGA+AGG | 0.575611 | 3.4:+22060901 | MS.gene82296:CDS |
| TGCGTTGAGGTGGTGGCCTG+CGG | 0.577755 | 3.4:-22069431 | None:intergenic |
| TAGTGCCCCAACCTCTGCAA+TGG | 0.580807 | 3.4:+22061339 | MS.gene82296:CDS |
| TTTCGATATGCATGACATGT+TGG | 0.582133 | 3.4:+22069464 | MS.gene82296:CDS |
| CCTCATCGTCATCGTCATCA+GGG | 0.582725 | 3.4:-22069719 | None:intergenic |
| TCGAATGGCTGCTATGCAAG+AGG | 0.583412 | 3.4:+22069758 | MS.gene82296:CDS |
| AAGATGATGCTTGAGAAGCT+CGG | 0.587317 | 3.4:-22060826 | None:intergenic |
| AGAGGCCATGGCTCTTGGGA+TGG | 0.587677 | 3.4:+22069686 | MS.gene82296:CDS |
| GGTCTTGTGCTTATGGTAGT+CGG | 0.588927 | 3.4:+22060786 | MS.gene82296:CDS |
| TTGGGTTCCGAAGGTTACAA+AGG | 0.589756 | 3.4:+22060910 | MS.gene82296:CDS |
| ACGCCACGCTTGTCAGGTGA+AGG | 0.590498 | 3.4:-22060935 | None:intergenic |
| GCTGCTGCCAACTGCCCCGG+CGG | 0.595992 | 3.4:+22061052 | MS.gene82296:CDS |
| AGCATCCATCCCAAGAGCCA+TGG | 0.600499 | 3.4:-22069691 | None:intergenic |
| TTATCAGATTCGCTGGCTGG+CGG | 0.606192 | 3.4:+22069585 | MS.gene82296:CDS |
| GTGAATACGAGAACATCCGC+CGG | 0.606994 | 3.4:-22061068 | None:intergenic |
| TGAAGCCTGGCTCATACACA+TGG | 0.611090 | 3.4:+22069403 | MS.gene82296:CDS |
| AAATGGAAATTGTCCATTTG+GGG | 0.614806 | 3.4:+22061631 | MS.gene82296:CDS |
| ATAACTACAAGGCAAACTGC+AGG | 0.617185 | 3.4:+22061479 | MS.gene82296:CDS |
| CATGACATGTTGGATGTGCT+CGG | 0.619477 | 3.4:+22069474 | MS.gene82296:CDS |
| GTAGTAAACCAAATACCACT+TGG | 0.621876 | 3.4:-22061430 | None:intergenic |
| GGAAATTATTGATAACTACA+AGG | 0.622897 | 3.4:+22061468 | MS.gene82296:CDS |
| TGTTTGACCGTTGAAAGAGG+AGG | 0.628307 | 3.4:+22070558 | MS.gene82296:CDS |
| GAATCAAAAGGACCCAAACA+AGG | 0.628395 | 3.4:-22069615 | None:intergenic |
| GGGCACTACTACGCCAATTG+CGG | 0.634177 | 3.4:-22061324 | None:intergenic |
| GGTGAGGTTGATCTATCAAG+TGG | 0.638628 | 3.4:+22069495 | MS.gene82296:CDS |
| TGACCGTTGAAAGAGGAGGG+AGG | 0.647609 | 3.4:+22070562 | MS.gene82296:CDS |
| ACTACCGATTTCCATTGCAG+AGG | 0.647818 | 3.4:-22061350 | None:intergenic |
| TAAGATTTACCTGTTGGACA+TGG | 0.648362 | 3.4:-22059790 | None:intergenic |
| CATACACATGGAGACACCGC+AGG | 0.651112 | 3.4:+22069415 | MS.gene82296:CDS |
| AGTACTGGATTCACCAACAT+CGG | 0.669244 | 3.4:-22060990 | None:intergenic |
| GTTCCTTCACCTGACAAGCG+TGG | 0.671133 | 3.4:+22060932 | MS.gene82296:CDS |
| CCCTGATGACGATGACGATG+AGG | 0.676584 | 3.4:+22069719 | MS.gene82296:CDS |
| CATGTTGGATGTGCTCGGTG+AGG | 0.698239 | 3.4:+22069479 | MS.gene82296:CDS |
| GTGATGGAAAGTCCAAATAG+CGG | 0.716896 | 3.4:-22070750 | None:intergenic |
| GAATACGAGAACATCCGCCG+GGG | 0.781384 | 3.4:-22061066 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 22059792 | 22070849 | 22059792 | ID=MS.gene82296 |
| chr3.4 | mRNA | 22059792 | 22070849 | 22059792 | ID=MS.gene82296.t1;Parent=MS.gene82296 |
| chr3.4 | exon | 22059792 | 22059802 | 22059792 | ID=MS.gene82296.t1.exon1;Parent=MS.gene82296.t1 |
| chr3.4 | CDS | 22059792 | 22059802 | 22059792 | ID=cds.MS.gene82296.t1;Parent=MS.gene82296.t1 |
| chr3.4 | exon | 22060423 | 22060507 | 22060423 | ID=MS.gene82296.t1.exon2;Parent=MS.gene82296.t1 |
| chr3.4 | CDS | 22060423 | 22060507 | 22060423 | ID=cds.MS.gene82296.t1;Parent=MS.gene82296.t1 |
| chr3.4 | exon | 22060764 | 22061500 | 22060764 | ID=MS.gene82296.t1.exon3;Parent=MS.gene82296.t1 |
| chr3.4 | CDS | 22060764 | 22061500 | 22060764 | ID=cds.MS.gene82296.t1;Parent=MS.gene82296.t1 |
| chr3.4 | exon | 22061601 | 22061670 | 22061601 | ID=MS.gene82296.t1.exon4;Parent=MS.gene82296.t1 |
| chr3.4 | CDS | 22061601 | 22061670 | 22061601 | ID=cds.MS.gene82296.t1;Parent=MS.gene82296.t1 |
| chr3.4 | exon | 22069396 | 22069785 | 22069396 | ID=MS.gene82296.t1.exon5;Parent=MS.gene82296.t1 |
| chr3.4 | CDS | 22069396 | 22069785 | 22069396 | ID=cds.MS.gene82296.t1;Parent=MS.gene82296.t1 |
| chr3.4 | exon | 22070555 | 22070611 | 22070555 | ID=MS.gene82296.t1.exon6;Parent=MS.gene82296.t1 |
| chr3.4 | CDS | 22070555 | 22070611 | 22070555 | ID=cds.MS.gene82296.t1;Parent=MS.gene82296.t1 |
| chr3.4 | exon | 22070739 | 22070849 | 22070739 | ID=MS.gene82296.t1.exon7;Parent=MS.gene82296.t1 |
| chr3.4 | CDS | 22070739 | 22070849 | 22070739 | ID=cds.MS.gene82296.t1;Parent=MS.gene82296.t1 |
| Gene Sequence |
| Protein sequence |
