Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82405.t1 | AES90030.2 | 87.2 | 156 | 20 | 0 | 10 | 165 | 38 | 193 | 4.50E-71 | 277.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82405.t1 | Q7XLP4 | 39.1 | 110 | 63 | 2 | 37 | 143 | 83 | 191 | 9.4e-17 | 88.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82405.t1 | G7JHL3 | 87.2 | 156 | 20 | 0 | 10 | 165 | 38 | 193 | 3.3e-71 | 277.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene82405.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82405.t1 | MTR_4g083390 | 87.179 | 156 | 20 | 0 | 10 | 165 | 38 | 193 | 1.47e-98 | 282 |
| MS.gene82405.t1 | MTR_4g083390 | 90.977 | 133 | 12 | 0 | 33 | 165 | 1 | 133 | 4.21e-88 | 253 |
| MS.gene82405.t1 | MTR_4g083390 | 85.496 | 131 | 19 | 0 | 33 | 163 | 1 | 131 | 2.68e-80 | 234 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGGAATTGAACCGCTTTC+AGG | 0.230161 | 4.4:+29356939 | MS.gene82405:CDS |
| TTCAAAGTTCAAATTCTTAG+AGG | 0.303256 | 4.4:+29357344 | MS.gene82405:CDS |
| AGTTGGGCCACGACTTATAA+TGG | 0.308961 | 4.4:+29357200 | MS.gene82405:CDS |
| ACGGGGACTAAGATGTGTTT+TGG | 0.312922 | 4.4:-29356988 | None:intergenic |
| TTCCGACTATTCTCAAATAT+CGG | 0.320685 | 4.4:+29357171 | MS.gene82405:CDS |
| GCATCCCTCTCACCGGTTCC+AGG | 0.356774 | 4.4:-29357398 | None:intergenic |
| TGAAATTACCAATCCATAAC+GGG | 0.384987 | 4.4:-29357006 | None:intergenic |
| GAGAATAGTCGGAACCGATT+TGG | 0.387243 | 4.4:-29357162 | None:intergenic |
| ACACATCTTAGTCCCCGTTA+TGG | 0.404304 | 4.4:+29356993 | MS.gene82405:CDS |
| GAAGATAATTATTTGAAGCT+TGG | 0.405476 | 4.4:+29357272 | MS.gene82405:CDS |
| TCCGACTATTCTCAAATATC+GGG | 0.408506 | 4.4:+29357172 | MS.gene82405:CDS |
| GCCAACGCTATCAAACAGTT+TGG | 0.414501 | 4.4:-29357234 | None:intergenic |
| AACTGTTTGATAGCGTTGGC+TGG | 0.415821 | 4.4:+29357237 | MS.gene82405:CDS |
| GAAAGGACCACATGAAAGTA+TGG | 0.437117 | 4.4:-29356966 | None:intergenic |
| CTTTACGAATTTCCTGGAAC+CGG | 0.450094 | 4.4:+29357386 | MS.gene82405:CDS |
| CAAATATCGGGGTAAGAGTT+GGG | 0.464634 | 4.4:+29357184 | MS.gene82405:CDS |
| GATTACTAATCTATGACAAA+TGG | 0.467072 | 4.4:-29357422 | None:intergenic |
| TCAAATATCGGGGTAAGAGT+TGG | 0.468409 | 4.4:+29357183 | MS.gene82405:CDS |
| TGAAGAACTTCCTTCCAAAT+CGG | 0.469161 | 4.4:+29357148 | MS.gene82405:CDS |
| GTTGGGCCACGACTTATAAT+GGG | 0.482324 | 4.4:+29357201 | MS.gene82405:CDS |
| ATGAAATTACCAATCCATAA+CGG | 0.499697 | 4.4:-29357007 | None:intergenic |
| ATGTCGTCTTCAAAATCAGC+TGG | 0.502794 | 4.4:+29356745 | MS.gene82405:CDS |
| ACCAAACTGTTTGATAGCGT+TGG | 0.508522 | 4.4:+29357233 | MS.gene82405:CDS |
| CCCCGATATTTGAGAATAGT+CGG | 0.515602 | 4.4:-29357173 | None:intergenic |
| TCTTAGTCCCCGTTATGGAT+TGG | 0.515821 | 4.4:+29356998 | MS.gene82405:CDS |
| TCCTTCGACAAATATGCAAA+CGG | 0.531644 | 4.4:+29356917 | MS.gene82405:CDS |
| GAAGGTGATGATAACGAGGC+TGG | 0.531657 | 4.4:-29356891 | None:intergenic |
| ACTGTTTGATAGCGTTGGCT+GGG | 0.539366 | 4.4:+29357238 | MS.gene82405:CDS |
| TCCGTTTGCATATTTGTCGA+AGG | 0.549975 | 4.4:-29356918 | None:intergenic |
| GTTCTGCCCATTATAAGTCG+TGG | 0.557426 | 4.4:-29357207 | None:intergenic |
| TCTTCAAAATCAGCTGGAAG+AGG | 0.564849 | 4.4:+29356751 | MS.gene82405:CDS |
| AAATGGTGCATCCCTCTCAC+CGG | 0.578086 | 4.4:-29357405 | None:intergenic |
| AGGAGTGCCATACTTTCATG+TGG | 0.578309 | 4.4:+29356959 | MS.gene82405:CDS |
| GAGTAGCAGTCAAGTTGCTG+TGG | 0.583739 | 4.4:+29356863 | MS.gene82405:CDS |
| TCTTCACATATGCTGCTGCT+CGG | 0.585483 | 4.4:-29357131 | None:intergenic |
| ATTTCCTGGAACCGGTGAGA+GGG | 0.587927 | 4.4:+29357394 | MS.gene82405:CDS |
| TCGACAAATATGCAAACGGA+TGG | 0.588200 | 4.4:+29356921 | MS.gene82405:CDS |
| AGTAGCAGTCAAGTTGCTGT+GGG | 0.603556 | 4.4:+29356864 | MS.gene82405:CDS |
| ATATTTGTCGAAGGAGCAGA+AGG | 0.614477 | 4.4:-29356909 | None:intergenic |
| AATTTCCTGGAACCGGTGAG+AGG | 0.633829 | 4.4:+29357393 | MS.gene82405:CDS |
| ATAGTCGGAACCGATTTGGA+AGG | 0.643344 | 4.4:-29357158 | None:intergenic |
| AAGTATGGCACTCCTGAAAG+CGG | 0.678549 | 4.4:-29356951 | None:intergenic |
| CCGACTATTCTCAAATATCG+GGG | 0.681942 | 4.4:+29357173 | MS.gene82405:CDS |
| AGCAGAAGGTGATGATAACG+AGG | 0.685363 | 4.4:-29356895 | None:intergenic |
| GAAATTACCAATCCATAACG+GGG | 0.716232 | 4.4:-29357005 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAATCTTGTATTGTATTGT+TGG | + | chr4.4:29357098-29357117 | MS.gene82405:intron | 20.0% |
| !! | TAATGTGATATGAAATACTA+AGG | - | chr4.4:29356784-29356803 | None:intergenic | 20.0% |
| !! | TTATAGATCAAACTAAATGA+AGG | + | chr4.4:29357047-29357066 | MS.gene82405:intron | 20.0% |
| ! | AAGATTAACTTTCATGTACA+CGG | + | chr4.4:29356806-29356825 | MS.gene82405:intron | 25.0% |
| ! | ATGAAATTACCAATCCATAA+CGG | - | chr4.4:29357010-29357029 | None:intergenic | 25.0% |
| ! | TATTGTATTGTTGGCATATA+GGG | + | chr4.4:29357107-29357126 | MS.gene82405:intron | 25.0% |
| ! | TTCAAAGTTCAAATTCTTAG+AGG | + | chr4.4:29357344-29357363 | MS.gene82405:CDS | 25.0% |
| !! | ATCAAACTAAATGAAGGTTT+TGG | + | chr4.4:29357053-29357072 | MS.gene82405:intron | 25.0% |
| !! | GAAGATAATTATTTGAAGCT+TGG | + | chr4.4:29357272-29357291 | MS.gene82405:CDS | 25.0% |
| ATTGTATTGTTGGCATATAG+GGG | + | chr4.4:29357108-29357127 | MS.gene82405:intron | 30.0% | |
| GGAAATTCGTAAAGCAAAAA+TGG | - | chr4.4:29357380-29357399 | None:intergenic | 30.0% | |
| GTATTGTATTGTTGGCATAT+AGG | + | chr4.4:29357106-29357125 | MS.gene82405:intron | 30.0% | |
| GTGTACATGAAAGTTAATCT+TGG | - | chr4.4:29356807-29356826 | None:intergenic | 30.0% | |
| TGAAATTACCAATCCATAAC+GGG | - | chr4.4:29357009-29357028 | None:intergenic | 30.0% | |
| TTCCGACTATTCTCAAATAT+CGG | + | chr4.4:29357171-29357190 | MS.gene82405:CDS | 30.0% | |
| ! | TTTTTGCTTTACGAATTTCC+TGG | + | chr4.4:29357380-29357399 | MS.gene82405:CDS | 30.0% |
| !!! | ACTAAATGAAGGTTTTGGAA+AGG | + | chr4.4:29357058-29357077 | MS.gene82405:intron | 30.0% |
| GAAATTACCAATCCATAACG+GGG | - | chr4.4:29357008-29357027 | None:intergenic | 35.0% | |
| TCCGACTATTCTCAAATATC+GGG | + | chr4.4:29357172-29357191 | MS.gene82405:CDS | 35.0% | |
| TCCTTCGACAAATATGCAAA+CGG | + | chr4.4:29356917-29356936 | MS.gene82405:CDS | 35.0% | |
| TGAAGAACTTCCTTCCAAAT+CGG | + | chr4.4:29357148-29357167 | MS.gene82405:CDS | 35.0% | |
| TTAACTTTCATGTACACGGT+CGG | + | chr4.4:29356810-29356829 | MS.gene82405:intron | 35.0% | |
| !!! | GACTAAGATGTGTTTTGGAA+AGG | - | chr4.4:29356986-29357005 | None:intergenic | 35.0% |
| ATATTTGTCGAAGGAGCAGA+AGG | - | chr4.4:29356912-29356931 | None:intergenic | 40.0% | |
| ATGTCGTCTTCAAAATCAGC+TGG | + | chr4.4:29356745-29356764 | MS.gene82405:CDS | 40.0% | |
| CAAATATCGGGGTAAGAGTT+GGG | + | chr4.4:29357184-29357203 | MS.gene82405:CDS | 40.0% | |
| CCCCGATATTTGAGAATAGT+CGG | - | chr4.4:29357176-29357195 | None:intergenic | 40.0% | |
| CCGACTATTCTCAAATATCG+GGG | + | chr4.4:29357173-29357192 | MS.gene82405:CDS | 40.0% | |
| CTTTACGAATTTCCTGGAAC+CGG | + | chr4.4:29357386-29357405 | MS.gene82405:CDS | 40.0% | |
| GAAAGGACCACATGAAAGTA+TGG | - | chr4.4:29356969-29356988 | None:intergenic | 40.0% | |
| GCTCATGAAAAACAGTGAAG+AGG | + | chr4.4:29357313-29357332 | MS.gene82405:CDS | 40.0% | |
| TCAAATATCGGGGTAAGAGT+TGG | + | chr4.4:29357183-29357202 | MS.gene82405:CDS | 40.0% | |
| TCCGTTTGCATATTTGTCGA+AGG | - | chr4.4:29356921-29356940 | None:intergenic | 40.0% | |
| TCGACAAATATGCAAACGGA+TGG | + | chr4.4:29356921-29356940 | MS.gene82405:CDS | 40.0% | |
| TCTTCAAAATCAGCTGGAAG+AGG | + | chr4.4:29356751-29356770 | MS.gene82405:CDS | 40.0% | |
| !! | ACCAAACTGTTTGATAGCGT+TGG | + | chr4.4:29357233-29357252 | MS.gene82405:CDS | 40.0% |
| AAGTATGGCACTCCTGAAAG+CGG | - | chr4.4:29356954-29356973 | None:intergenic | 45.0% | |
| ACACATCTTAGTCCCCGTTA+TGG | + | chr4.4:29356993-29357012 | MS.gene82405:CDS | 45.0% | |
| AGTTGGGCCACGACTTATAA+TGG | + | chr4.4:29357200-29357219 | MS.gene82405:CDS | 45.0% | |
| GAGAATAGTCGGAACCGATT+TGG | - | chr4.4:29357165-29357184 | None:intergenic | 45.0% | |
| GATGGAATTGAACCGCTTTC+AGG | + | chr4.4:29356939-29356958 | MS.gene82405:CDS | 45.0% | |
| GCCAACGCTATCAAACAGTT+TGG | - | chr4.4:29357237-29357256 | None:intergenic | 45.0% | |
| GTTCTGCCCATTATAAGTCG+TGG | - | chr4.4:29357210-29357229 | None:intergenic | 45.0% | |
| GTTGGGCCACGACTTATAAT+GGG | + | chr4.4:29357201-29357220 | MS.gene82405:CDS | 45.0% | |
| TCTTAGTCCCCGTTATGGAT+TGG | + | chr4.4:29356998-29357017 | MS.gene82405:CDS | 45.0% | |
| TCTTCACATATGCTGCTGCT+CGG | - | chr4.4:29357134-29357153 | None:intergenic | 45.0% | |
| ! | ACGGGGACTAAGATGTGTTT+TGG | - | chr4.4:29356991-29357010 | None:intergenic | 45.0% |
| ! | AGCAGAAGGTGATGATAACG+AGG | - | chr4.4:29356898-29356917 | None:intergenic | 45.0% |
| ! | AGTAGCAGTCAAGTTGCTGT+GGG | + | chr4.4:29356864-29356883 | MS.gene82405:CDS | 45.0% |
| !! | AACTGTTTGATAGCGTTGGC+TGG | + | chr4.4:29357237-29357256 | MS.gene82405:CDS | 45.0% |
| !! | ACTGTTTGATAGCGTTGGCT+GGG | + | chr4.4:29357238-29357257 | MS.gene82405:CDS | 45.0% |
| !! | AGGAGTGCCATACTTTCATG+TGG | + | chr4.4:29356959-29356978 | MS.gene82405:CDS | 45.0% |
| !! | ATAGTCGGAACCGATTTGGA+AGG | - | chr4.4:29357161-29357180 | None:intergenic | 45.0% |
| AAATGGTGCATCCCTCTCAC+CGG | - | chr4.4:29357408-29357427 | None:intergenic | 50.0% | |
| AATTTCCTGGAACCGGTGAG+AGG | + | chr4.4:29357393-29357412 | MS.gene82405:CDS | 50.0% | |
| ATTTCCTGGAACCGGTGAGA+GGG | + | chr4.4:29357394-29357413 | MS.gene82405:CDS | 50.0% | |
| ! | GAAGGTGATGATAACGAGGC+TGG | - | chr4.4:29356894-29356913 | None:intergenic | 50.0% |
| ! | GAGTAGCAGTCAAGTTGCTG+TGG | + | chr4.4:29356863-29356882 | MS.gene82405:CDS | 50.0% |
| !! | GCATCCCTCTCACCGGTTCC+AGG | - | chr4.4:29357401-29357420 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 29356745 | 29357439 | 29356745 | ID=MS.gene82405 |
| chr4.4 | mRNA | 29356745 | 29357439 | 29356745 | ID=MS.gene82405.t1;Parent=MS.gene82405 |
| chr4.4 | exon | 29356745 | 29356772 | 29356745 | ID=MS.gene82405.t1.exon1;Parent=MS.gene82405.t1 |
| chr4.4 | CDS | 29356745 | 29356772 | 29356745 | ID=cds.MS.gene82405.t1;Parent=MS.gene82405.t1 |
| chr4.4 | exon | 29356862 | 29357019 | 29356862 | ID=MS.gene82405.t1.exon2;Parent=MS.gene82405.t1 |
| chr4.4 | CDS | 29356862 | 29357019 | 29356862 | ID=cds.MS.gene82405.t1;Parent=MS.gene82405.t1 |
| chr4.4 | exon | 29357128 | 29357439 | 29357128 | ID=MS.gene82405.t1.exon3;Parent=MS.gene82405.t1 |
| chr4.4 | CDS | 29357128 | 29357439 | 29357128 | ID=cds.MS.gene82405.t1;Parent=MS.gene82405.t1 |
| Gene Sequence |
| Protein sequence |
