Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82689.t1 | XP_024640623.1 | 93.2 | 205 | 14 | 0 | 1 | 205 | 1 | 205 | 4.70E-102 | 380.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82689.t1 | Q9SZL8 | 28.4 | 176 | 109 | 7 | 41 | 204 | 70 | 240 | 6.2e-10 | 65.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82689.t1 | A0A072TDR2 | 93.7 | 158 | 10 | 0 | 20 | 177 | 1 | 158 | 2.6e-78 | 301.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene82689.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82689.t1 | MTR_0853s0010 | 93.671 | 158 | 10 | 0 | 20 | 177 | 1 | 158 | 2.17e-109 | 310 |
| MS.gene82689.t1 | MTR_1g019850 | 52.381 | 168 | 67 | 2 | 18 | 179 | 38 | 198 | 6.76e-54 | 172 |
| MS.gene82689.t1 | MTR_2g024230 | 43.137 | 102 | 43 | 1 | 17 | 103 | 48 | 149 | 3.76e-21 | 85.9 |
| MS.gene82689.t1 | MTR_1g033820 | 45.333 | 75 | 40 | 1 | 32 | 105 | 15 | 89 | 1.09e-16 | 72.4 |
| MS.gene82689.t1 | MTR_6g048540 | 39.394 | 99 | 56 | 2 | 38 | 134 | 22 | 118 | 2.55e-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82689.t1 | AT4G38180 | 27.273 | 176 | 111 | 6 | 41 | 204 | 70 | 240 | 3.89e-12 | 65.1 |
Find 23 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTTCGGCATATGATTT+TGG | 0.142338 | 3.1:-37901437 | None:intergenic |
| TATTGATCCTTGTATTGATA+TGG | 0.239238 | 3.1:+37901675 | MS.gene82689:CDS |
| CCAAGAGTATAAACTATCTT+AGG | 0.313740 | 3.1:+37901998 | MS.gene82689:CDS |
| CCTAAGATAGTTTATACTCT+TGG | 0.340102 | 3.1:-37901998 | None:intergenic |
| CTAAGATAGTTTATACTCTT+GGG | 0.346829 | 3.1:-37901997 | None:intergenic |
| CATGTTATGACTATCTTCTT+CGG | 0.402279 | 3.1:-37901450 | None:intergenic |
| AACATCAAGAACTGATTGTA+AGG | 0.444776 | 3.1:+37901888 | MS.gene82689:CDS |
| ATATTTGTTTGCTCTAATGC+TGG | 0.447741 | 3.1:+37901796 | MS.gene82689:CDS |
| TGATCATCTAAGTCATGCTC+AGG | 0.470772 | 3.1:-37901640 | None:intergenic |
| AATGTTTAGCAATGAAGGTT+CGG | 0.471515 | 3.1:+37902104 | MS.gene82689:CDS |
| GATCAAGAGGGCTTGCCTAC+CGG | 0.495549 | 3.1:+37902069 | MS.gene82689:CDS |
| GCTGCAATGTTTAGCAATGA+AGG | 0.497956 | 3.1:+37902099 | MS.gene82689:CDS |
| GTATTGTTAAATGTAATGAT+AGG | 0.499697 | 3.1:-37901952 | None:intergenic |
| TGTCATTACTATCAACATGT+TGG | 0.533945 | 3.1:-37901819 | None:intergenic |
| TCATAACATGGAGGACATAG+AGG | 0.553789 | 3.1:+37901463 | MS.gene82689:CDS |
| AGTTGAACAGTTTGATCAAG+AGG | 0.557965 | 3.1:+37902056 | MS.gene82689:CDS |
| TAAGATAGTTTATACTCTTG+GGG | 0.560800 | 3.1:-37901996 | None:intergenic |
| GTTGAACAGTTTGATCAAGA+GGG | 0.578599 | 3.1:+37902057 | MS.gene82689:CDS |
| CGAAGAAGATAGTCATAACA+TGG | 0.604552 | 3.1:+37901451 | MS.gene82689:CDS |
| CAATACTCATAATCATGCAA+TGG | 0.611156 | 3.1:+37901969 | MS.gene82689:CDS |
| TCAAATTCCATATCAATACA+AGG | 0.614636 | 3.1:-37901682 | None:intergenic |
| GATCATCTAAGTCATGCTCA+GGG | 0.668219 | 3.1:-37901639 | None:intergenic |
| AGAAGATAGTCATAACATGG+AGG | 0.736903 | 3.1:+37901454 | MS.gene82689:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTATTGTTAAATGTAATGAT+AGG | - | chr3.1:37901955-37901974 | None:intergenic | 20.0% |
| ! | CTAAGATAGTTTATACTCTT+GGG | - | chr3.1:37902000-37902019 | None:intergenic | 25.0% |
| ! | TAAGATAGTTTATACTCTTG+GGG | - | chr3.1:37901999-37902018 | None:intergenic | 25.0% |
| ! | TAGTTTCAAAAGCAAAAATG+AGG | + | chr3.1:37901920-37901939 | MS.gene82689:CDS | 25.0% |
| ! | TCAAATTCCATATCAATACA+AGG | - | chr3.1:37901685-37901704 | None:intergenic | 25.0% |
| !! | TATTGATCCTTGTATTGATA+TGG | + | chr3.1:37901675-37901694 | MS.gene82689:CDS | 25.0% |
| !! | TTTGCTAAAAAAGAAGGTTT+TGG | + | chr3.1:37901739-37901758 | MS.gene82689:CDS | 25.0% |
| AACATCAAGAACTGATTGTA+AGG | + | chr3.1:37901888-37901907 | MS.gene82689:CDS | 30.0% | |
| AATGTTTAGCAATGAAGGTT+CGG | + | chr3.1:37902104-37902123 | MS.gene82689:CDS | 30.0% | |
| ATATTTGTTTGCTCTAATGC+TGG | + | chr3.1:37901796-37901815 | MS.gene82689:CDS | 30.0% | |
| CAATACTCATAATCATGCAA+TGG | + | chr3.1:37901969-37901988 | MS.gene82689:CDS | 30.0% | |
| CATGTTATGACTATCTTCTT+CGG | - | chr3.1:37901453-37901472 | None:intergenic | 30.0% | |
| CCTAAGATAGTTTATACTCT+TGG | - | chr3.1:37902001-37902020 | None:intergenic | 30.0% | |
| TGTCATTACTATCAACATGT+TGG | - | chr3.1:37901822-37901841 | None:intergenic | 30.0% | |
| ! | ACGTCATTTGCTAAAAAAGA+AGG | + | chr3.1:37901733-37901752 | MS.gene82689:CDS | 30.0% |
| ! | CCAAGAGTATAAACTATCTT+AGG | + | chr3.1:37901998-37902017 | MS.gene82689:CDS | 30.0% |
| ! | CTTAGGTGCCATAAAAAAAT+GGG | + | chr3.1:37902015-37902034 | MS.gene82689:CDS | 30.0% |
| ! | CTTTCTCTGATAGAGATTTT+TGG | + | chr3.1:37902127-37902146 | MS.gene82689:CDS | 30.0% |
| ! | TCTTAGGTGCCATAAAAAAA+TGG | + | chr3.1:37902014-37902033 | MS.gene82689:CDS | 30.0% |
| AGAAGATAGTCATAACATGG+AGG | + | chr3.1:37901454-37901473 | MS.gene82689:CDS | 35.0% | |
| AGTTGAACAGTTTGATCAAG+AGG | + | chr3.1:37902056-37902075 | MS.gene82689:CDS | 35.0% | |
| CGAAGAAGATAGTCATAACA+TGG | + | chr3.1:37901451-37901470 | MS.gene82689:CDS | 35.0% | |
| GTTGAACAGTTTGATCAAGA+GGG | + | chr3.1:37902057-37902076 | MS.gene82689:CDS | 35.0% | |
| ! | TCTTCTTCGGCATATGATTT+TGG | - | chr3.1:37901440-37901459 | None:intergenic | 35.0% |
| AAGCAAAAATGAGGCGCAAA+TGG | + | chr3.1:37901929-37901948 | MS.gene82689:CDS | 40.0% | |
| GATCATCTAAGTCATGCTCA+GGG | - | chr3.1:37901642-37901661 | None:intergenic | 40.0% | |
| GCTGCAATGTTTAGCAATGA+AGG | + | chr3.1:37902099-37902118 | MS.gene82689:CDS | 40.0% | |
| GTGCCATAAAAAAATGGGTG+GGG | + | chr3.1:37902020-37902039 | MS.gene82689:CDS | 40.0% | |
| TCATAACATGGAGGACATAG+AGG | + | chr3.1:37901463-37901482 | MS.gene82689:CDS | 40.0% | |
| TGATCATCTAAGTCATGCTC+AGG | - | chr3.1:37901643-37901662 | None:intergenic | 40.0% | |
| ! | AAACATTGCAGCGACTTTTC+CGG | - | chr3.1:37902091-37902110 | None:intergenic | 40.0% |
| ! | AGGTGCCATAAAAAAATGGG+TGG | + | chr3.1:37902018-37902037 | MS.gene82689:CDS | 40.0% |
| ! | GGTGCCATAAAAAAATGGGT+GGG | + | chr3.1:37902019-37902038 | MS.gene82689:CDS | 40.0% |
| !!! | CTGCCCCACCCATTTTTTTA+TGG | - | chr3.1:37902026-37902045 | None:intergenic | 45.0% |
| ! | ATTGCAGCGACTTTTCCGGT+AGG | - | chr3.1:37902087-37902106 | None:intergenic | 50.0% |
| GATCAAGAGGGCTTGCCTAC+CGG | + | chr3.1:37902069-37902088 | MS.gene82689:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 37901428 | 37902170 | 37901428 | ID=MS.gene82689 |
| chr3.1 | mRNA | 37901428 | 37902170 | 37901428 | ID=MS.gene82689.t1;Parent=MS.gene82689 |
| chr3.1 | exon | 37901428 | 37901484 | 37901428 | ID=MS.gene82689.t1.exon1;Parent=MS.gene82689.t1 |
| chr3.1 | CDS | 37901428 | 37901484 | 37901428 | ID=cds.MS.gene82689.t1;Parent=MS.gene82689.t1 |
| chr3.1 | exon | 37901610 | 37902170 | 37901610 | ID=MS.gene82689.t1.exon2;Parent=MS.gene82689.t1 |
| chr3.1 | CDS | 37901610 | 37902170 | 37901610 | ID=cds.MS.gene82689.t1;Parent=MS.gene82689.t1 |
| Gene Sequence |
| Protein sequence |
