AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82944

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82944.t1 MTR_2g011820 90.476 105 10 0 2 106 380 484 8.10e-61 195
MS.gene82944.t1 MTR_7g110060 96.226 53 2 0 107 159 240 292 9.44e-29 109
MS.gene82944.t1 MTR_7g110060 96.226 53 2 0 107 159 240 292 1.30e-28 109
MS.gene82944.t1 MTR_1g086370 92.453 53 4 0 107 159 121 173 4.74e-28 105
MS.gene82944.t1 MTR_1g086370 92.453 53 4 0 107 159 207 259 1.44e-27 106
MS.gene82944.t1 MTR_7g076030 90.566 53 5 0 107 159 199 251 1.53e-27 105
MS.gene82944.t1 MTR_7g076030 90.566 53 5 0 107 159 306 358 1.83e-27 107
MS.gene82944.t1 MTR_1g086370 92.453 53 4 0 107 159 240 292 2.05e-27 106
MS.gene82944.t1 MTR_1g084760 90.566 53 5 0 107 159 240 292 1.15e-26 104
MS.gene82944.t1 MTR_2g005620 90.566 53 5 0 107 159 255 307 1.39e-26 103
MS.gene82944.t1 MTR_2g083940 90.566 53 5 0 107 159 199 251 4.86e-26 102
MS.gene82944.t1 MTR_2g083940 90.566 53 5 0 107 159 253 305 7.25e-26 102
MS.gene82944.t1 MTR_8g432510 86.792 53 7 0 107 159 304 356 1.83e-25 101
MS.gene82944.t1 MTR_2g016490 86.792 53 7 0 107 159 205 257 1.88e-25 100
MS.gene82944.t1 MTR_7g114300 47.368 76 28 1 95 158 248 323 3.79e-16 75.1
MS.gene82944.t1 MTR_7g114300 47.368 76 28 1 95 158 248 323 4.81e-16 74.7
MS.gene82944.t1 MTR_7g114300 47.368 76 28 1 95 158 248 323 5.05e-16 74.7
MS.gene82944.t1 MTR_5g008860 44.000 75 30 1 96 158 282 356 5.16e-16 74.7
MS.gene82944.t1 MTR_7g114300 47.368 76 28 1 95 158 248 323 5.23e-16 74.7
MS.gene82944.t1 MTR_2g020210 41.860 86 38 1 85 158 233 318 8.71e-16 74.3
MS.gene82944.t1 MTR_4g118780 45.333 75 29 1 96 158 246 320 1.35e-15 73.6
MS.gene82944.t1 MTR_1g081870 44.737 76 30 1 95 158 263 338 2.17e-15 73.2
MS.gene82944.t1 MTR_3g098760 44.737 76 30 1 95 158 238 313 1.43e-14 70.5
MS.gene82944.t1 MTR_2g085200 42.667 75 31 1 96 158 276 350 1.65e-14 70.5
MS.gene82944.t1 MTR_2g032060 44.444 54 30 0 106 159 167 220 4.54e-14 68.6
MS.gene82944.t1 MTR_4g094430 43.396 53 30 0 106 158 167 219 6.38e-14 68.2
MS.gene82944.t1 MTR_3g085650 47.458 59 31 0 101 159 574 632 8.30e-14 68.6
MS.gene82944.t1 MTR_3g085650 47.458 59 31 0 101 159 574 632 8.37e-14 68.6
MS.gene82944.t1 MTR_2g090405 28.221 163 92 4 10 158 206 357 8.98e-14 68.6
MS.gene82944.t1 MTR_3g085650 47.458 59 31 0 101 159 574 632 9.15e-14 68.6
MS.gene82944.t1 MTR_3g085650 47.458 59 31 0 101 159 574 632 9.59e-14 68.2
MS.gene82944.t1 MTR_8g080190 48.276 58 30 0 102 159 544 601 9.61e-14 68.2
MS.gene82944.t1 MTR_8g080190 48.276 58 30 0 102 159 544 601 1.08e-13 68.2
MS.gene82944.t1 MTR_8g099770 45.333 75 29 2 96 158 232 306 1.09e-13 68.2
MS.gene82944.t1 MTR_4g007750 48.148 54 28 0 106 159 184 237 2.53e-13 66.6
MS.gene82944.t1 MTR_8g028720 58.696 46 19 0 107 152 202 247 3.58e-13 66.2
MS.gene82944.t1 MTR_7g078690 46.667 60 32 0 107 166 203 262 4.95e-13 65.9
MS.gene82944.t1 MTR_4g078290 43.333 60 34 0 99 158 286 345 5.01e-13 66.2
MS.gene82944.t1 MTR_4g078290 43.333 60 34 0 99 158 286 345 5.06e-13 66.2
MS.gene82944.t1 MTR_8g092290 38.571 70 43 0 89 158 288 357 5.54e-13 66.2
MS.gene82944.t1 MTR_4g061330 49.057 53 27 0 107 159 9 61 5.84e-13 63.5
MS.gene82944.t1 MTR_6g087920 44.615 65 36 0 95 159 267 331 6.69e-13 65.9
MS.gene82944.t1 MTR_4g103810 44.068 59 33 0 101 159 378 436 6.90e-13 65.9
MS.gene82944.t1 MTR_4g103810 44.068 59 33 0 101 159 268 326 8.68e-13 65.5
MS.gene82944.t1 MTR_8g098845 43.333 60 34 0 107 166 217 276 1.02e-12 65.1
MS.gene82944.t1 MTR_8g103240 50.943 53 26 0 107 159 292 344 1.04e-12 65.1
MS.gene82944.t1 MTR_1g075610 39.474 76 35 1 95 159 167 242 1.06e-12 64.7
MS.gene82944.t1 MTR_1g098300 42.857 63 36 0 96 158 280 342 1.12e-12 65.1
MS.gene82944.t1 MTR_8g070930 52.830 53 24 1 107 159 307 358 1.14e-12 65.5
MS.gene82944.t1 MTR_4g109090 41.935 62 36 0 97 158 112 173 1.70e-12 64.7
MS.gene82944.t1 MTR_5g010030 43.333 60 34 0 107 166 206 265 2.05e-12 64.3
MS.gene82944.t1 MTR_8g086000 50.943 53 26 0 107 159 198 250 2.25e-12 63.9
MS.gene82944.t1 MTR_8g086000 50.943 53 26 0 107 159 241 293 2.34e-12 64.3
MS.gene82944.t1 MTR_4g061320 49.057 53 27 0 107 159 205 257 2.41e-12 63.9
MS.gene82944.t1 MTR_4g109090 41.935 62 36 0 97 158 260 321 2.78e-12 63.9
MS.gene82944.t1 MTR_6g013030 43.333 60 34 0 99 158 267 326 3.78e-12 63.5
MS.gene82944.t1 MTR_7g011800 37.333 75 35 1 96 158 262 336 1.31e-11 62.0
MS.gene82944.t1 MTR_3g460950 45.614 57 31 0 107 163 198 254 2.29e-11 61.2
MS.gene82944.t1 MTR_3g060330 45.614 57 31 0 107 163 198 254 2.29e-11 61.2
MS.gene82944.t1 MTR_3g040810 43.396 53 30 0 106 158 201 253 3.15e-11 60.8
MS.gene82944.t1 MTR_8g461040 54.545 55 22 2 107 159 195 248 3.36e-11 60.8
MS.gene82944.t1 MTR_3g040520 43.396 53 30 0 106 158 203 255 3.91e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82944.t1 AT4G21540 70.192 104 31 0 2 105 171 274 1.49e-49 161
MS.gene82944.t1 AT4G21540 70.192 104 31 0 2 105 381 484 1.84e-47 160
MS.gene82944.t1 AT4G21534 69.811 106 32 0 2 107 376 481 2.90e-47 160
MS.gene82944.t1 AT4G21534 69.811 106 32 0 2 107 376 481 2.90e-47 160
MS.gene82944.t1 AT5G14640 94.340 53 3 0 107 159 239 291 9.36e-28 107
MS.gene82944.t1 AT5G14640 94.340 53 3 0 107 159 239 291 9.36e-28 107
MS.gene82944.t1 AT3G05840 92.453 53 4 0 107 159 238 290 1.58e-27 106
MS.gene82944.t1 AT3G05840 92.453 53 4 0 107 159 238 290 1.58e-27 106
MS.gene82944.t1 AT5G26751 92.453 53 4 0 107 159 234 286 2.32e-27 105
MS.gene82944.t1 AT1G09840 88.679 53 6 0 107 159 121 173 5.95e-27 103
MS.gene82944.t1 AT1G57870 90.566 53 5 0 107 159 247 299 1.02e-26 104
MS.gene82944.t1 AT1G57870 90.566 53 5 0 107 159 247 299 1.02e-26 104
MS.gene82944.t1 AT1G57870 90.566 53 5 0 107 159 247 299 1.02e-26 104
MS.gene82944.t1 AT4G18710 88.679 53 6 0 107 159 199 251 1.06e-26 102
MS.gene82944.t1 AT1G57870 90.566 53 5 0 107 159 247 299 1.25e-26 104
MS.gene82944.t1 AT1G09840 88.679 53 6 0 107 159 248 300 2.64e-26 103
MS.gene82944.t1 AT1G09840 88.679 53 6 0 107 159 248 300 2.64e-26 103
MS.gene82944.t1 AT1G09840 88.679 53 6 0 107 159 248 300 2.64e-26 103
MS.gene82944.t1 AT1G09840 88.679 53 6 0 107 159 248 300 2.64e-26 103
MS.gene82944.t1 AT1G09840 88.679 53 6 0 107 159 248 300 2.64e-26 103
MS.gene82944.t1 AT1G09840 88.679 53 6 0 107 159 248 300 2.64e-26 103
MS.gene82944.t1 AT1G09840 88.679 53 6 0 107 159 248 300 2.64e-26 103
MS.gene82944.t1 AT1G09840 88.679 53 6 0 107 159 248 300 2.64e-26 103
MS.gene82944.t1 AT1G09840 88.679 53 6 0 107 159 248 300 2.64e-26 103
MS.gene82944.t1 AT1G09840 88.679 53 6 0 107 159 248 300 2.64e-26 103
MS.gene82944.t1 AT4G18710 88.679 53 6 0 107 159 205 257 6.45e-26 101
MS.gene82944.t1 AT2G30980 88.679 53 6 0 107 159 237 289 1.34e-25 101
MS.gene82944.t1 AT1G06390 88.679 53 6 0 107 159 235 287 1.54e-25 101
MS.gene82944.t1 AT1G06390 88.679 53 6 0 107 159 235 287 1.54e-25 101
MS.gene82944.t1 AT4G00720 83.019 53 9 0 107 159 303 355 2.17e-25 101
MS.gene82944.t1 AT3G61160 77.358 53 12 0 107 159 267 319 3.82e-24 96.7
MS.gene82944.t1 AT3G61160 77.358 53 12 0 107 159 267 319 4.01e-24 97.1
MS.gene82944.t1 AT3G61160 77.358 53 12 0 107 159 274 326 4.84e-24 96.7
MS.gene82944.t1 AT3G61160 77.358 53 12 0 107 159 267 319 8.45e-24 96.7
MS.gene82944.t1 AT3G61160 77.358 53 12 0 107 159 267 319 1.01e-23 96.3
MS.gene82944.t1 AT3G61160 77.358 53 12 0 107 159 274 326 1.07e-23 96.3
MS.gene82944.t1 AT1G54610 48.000 75 27 1 96 158 260 334 3.06e-16 75.5
MS.gene82944.t1 AT1G54610 48.000 75 27 1 96 158 260 334 3.06e-16 75.5
MS.gene82944.t1 AT1G54610 48.000 75 27 1 96 158 260 334 3.06e-16 75.5
MS.gene82944.t1 AT4G10010 50.000 62 31 0 96 157 130 191 1.91e-15 73.2
MS.gene82944.t1 AT5G50860 40.698 86 39 1 85 158 245 330 2.50e-15 72.8
MS.gene82944.t1 AT2G46070 51.667 60 29 0 107 166 206 265 3.09e-15 72.0
MS.gene82944.t1 AT5G50860 40.698 86 39 1 85 158 245 330 3.39e-15 72.4
MS.gene82944.t1 AT4G10010 50.000 62 31 0 96 157 310 371 3.76e-15 72.4
MS.gene82944.t1 AT2G46070 51.667 60 29 0 107 166 240 299 4.40e-15 72.0
MS.gene82944.t1 AT2G46070 51.667 60 29 0 107 166 206 265 4.70e-15 71.6
MS.gene82944.t1 AT3G48750 46.296 54 29 0 106 159 167 220 4.82e-15 71.2
MS.gene82944.t1 AT5G63370 54.717 53 24 0 107 159 466 518 6.28e-15 71.6
MS.gene82944.t1 AT5G63370 54.717 53 24 0 107 159 466 518 6.28e-15 71.6
MS.gene82944.t1 AT2G38620 40.659 91 40 3 82 159 148 237 7.03e-15 70.5
MS.gene82944.t1 AT2G38620 40.659 91 40 3 82 159 148 237 7.03e-15 70.5
MS.gene82944.t1 AT5G63370 54.717 53 24 0 107 159 466 518 7.43e-15 71.6
MS.gene82944.t1 AT5G63370 54.717 53 24 0 107 159 466 518 7.43e-15 71.6
MS.gene82944.t1 AT5G63370 54.717 53 24 0 107 159 466 518 7.43e-15 71.6
MS.gene82944.t1 AT5G63370 54.717 53 24 0 107 159 466 518 7.43e-15 71.6
MS.gene82944.t1 AT5G63370 54.717 53 24 0 107 159 466 518 7.43e-15 71.6
MS.gene82944.t1 AT5G63370 54.717 53 24 0 107 159 332 384 8.76e-15 71.2
MS.gene82944.t1 AT5G63370 54.717 53 24 0 107 159 332 384 1.02e-14 71.2
MS.gene82944.t1 AT5G63370 54.717 53 24 0 107 159 332 384 1.02e-14 71.2
MS.gene82944.t1 AT2G38620 40.659 91 40 3 82 159 86 175 1.20e-14 69.7
MS.gene82944.t1 AT1G71530 47.619 63 33 0 96 158 301 363 1.39e-14 70.9
MS.gene82944.t1 AT1G53050 42.667 75 31 1 96 158 276 350 1.66e-14 70.5
MS.gene82944.t1 AT1G53050 42.667 75 31 1 96 158 276 350 1.66e-14 70.5
MS.gene82944.t1 AT2G38620 40.659 91 40 3 82 159 148 237 1.69e-14 69.7
MS.gene82944.t1 AT1G03740 42.667 75 31 1 96 158 355 429 1.79e-14 70.5
MS.gene82944.t1 AT1G71530 47.619 63 33 0 96 158 301 363 1.80e-14 70.5
MS.gene82944.t1 AT1G71530 47.619 63 33 0 96 158 301 363 1.87e-14 70.5
MS.gene82944.t1 AT1G03740 42.667 75 31 1 96 158 355 429 2.11e-14 70.5
MS.gene82944.t1 AT1G33770 44.444 63 35 0 96 158 295 357 3.11e-14 69.7
MS.gene82944.t1 AT4G22940 39.535 86 40 1 85 158 235 320 3.23e-14 69.7
MS.gene82944.t1 AT4G22940 39.535 86 40 1 85 158 235 320 3.74e-14 69.7
MS.gene82944.t1 AT1G76540 43.421 76 32 2 95 159 163 238 3.87e-14 68.9
MS.gene82944.t1 AT1G67580 48.276 58 30 0 102 159 565 622 3.87e-14 69.7
MS.gene82944.t1 AT1G67580 48.276 58 30 0 102 159 565 622 3.87e-14 69.7
MS.gene82944.t1 AT1G01560 48.333 60 31 0 107 166 205 264 1.91e-13 67.0
MS.gene82944.t1 AT1G09600 41.935 62 36 0 97 158 318 379 2.02e-13 67.4
MS.gene82944.t1 AT1G20930 43.421 76 32 2 95 159 165 240 2.14e-13 67.0
MS.gene82944.t1 AT1G01560 53.846 52 24 0 107 158 205 256 2.17e-13 66.6
MS.gene82944.t1 AT1G01560 53.846 52 24 0 107 158 205 256 2.17e-13 66.6
MS.gene82944.t1 AT1G18670 45.161 62 34 0 97 158 287 348 2.28e-13 67.4
MS.gene82944.t1 AT1G57700 41.935 62 36 0 97 158 301 362 2.37e-13 67.4
MS.gene82944.t1 AT1G01560 48.333 60 31 0 107 166 205 264 2.71e-13 66.6
MS.gene82944.t1 AT1G18670 45.161 62 34 0 97 158 287 348 2.83e-13 67.0
MS.gene82944.t1 AT1G18670 45.161 62 34 0 97 158 287 348 2.86e-13 67.0
MS.gene82944.t1 AT1G74330 27.273 154 107 2 10 158 186 339 2.92e-13 67.0
MS.gene82944.t1 AT3G54180 37.778 90 44 2 82 159 146 235 2.98e-13 66.2
MS.gene82944.t1 AT1G74330 27.273 154 107 2 10 158 186 339 3.48e-13 66.6
MS.gene82944.t1 AT1G74330 27.273 154 107 2 10 158 186 339 3.55e-13 66.6
MS.gene82944.t1 AT3G01085 40.000 75 33 1 96 158 257 331 3.62e-13 66.6
MS.gene82944.t1 AT3G01085 40.000 75 33 1 96 158 257 331 3.62e-13 66.6
MS.gene82944.t1 AT3G01085 40.000 75 33 1 96 158 257 331 3.62e-13 66.6
MS.gene82944.t1 AT4G01370 48.333 60 31 0 107 166 208 267 4.70e-13 66.2
MS.gene82944.t1 AT5G35980 46.875 64 33 1 96 159 243 305 6.15e-13 66.2
MS.gene82944.t1 AT5G35980 46.875 64 33 1 96 159 278 340 6.18e-13 66.2
MS.gene82944.t1 AT5G35980 46.875 64 33 1 96 159 278 340 6.76e-13 65.9
MS.gene82944.t1 AT5G44290 40.789 76 33 1 95 158 278 353 8.46e-13 65.5
MS.gene82944.t1 AT5G44290 40.789 76 33 1 95 158 278 353 8.46e-13 65.5
MS.gene82944.t1 AT5G44290 40.789 76 33 1 95 158 278 353 8.46e-13 65.5
MS.gene82944.t1 AT5G44290 40.789 76 33 1 95 158 278 353 8.46e-13 65.5
MS.gene82944.t1 AT5G44290 40.789 76 33 1 95 158 278 353 8.46e-13 65.5
MS.gene82944.t1 AT1G73690 47.170 53 28 0 107 159 173 225 8.93e-13 65.5
MS.gene82944.t1 AT5G44290 41.333 75 32 1 96 158 279 353 9.63e-13 65.5
MS.gene82944.t1 AT5G39420 38.667 75 34 1 96 158 247 321 9.77e-13 65.5
MS.gene82944.t1 AT1G66750 40.789 76 34 2 95 159 152 227 1.02e-12 65.1
MS.gene82944.t1 AT5G44290 41.333 75 32 1 96 158 279 353 1.23e-12 65.1
MS.gene82944.t1 AT3G05050 41.333 75 32 1 96 158 280 354 1.60e-12 64.7
MS.gene82944.t1 AT3G05050 41.333 75 32 1 96 158 280 354 1.60e-12 64.7
MS.gene82944.t1 AT3G05050 41.333 75 32 1 96 158 280 354 1.60e-12 64.7
MS.gene82944.t1 AT4G11330 50.000 46 23 0 107 152 208 253 3.26e-12 63.5
MS.gene82944.t1 AT1G71530 50.000 54 27 0 96 149 301 354 3.31e-12 63.5
MS.gene82944.t1 AT3G59790 43.396 53 30 0 107 159 225 277 8.23e-12 62.8
MS.gene82944.t1 AT1G18040 43.396 53 30 0 107 159 174 226 1.21e-11 62.0
MS.gene82944.t1 AT4G28980 42.105 76 42 2 86 159 274 349 1.82e-11 61.6
MS.gene82944.t1 AT4G28980 42.105 76 42 2 86 159 274 349 1.82e-11 61.6
MS.gene82944.t1 AT4G28980 42.105 76 42 2 86 159 274 349 1.82e-11 61.6
MS.gene82944.t1 AT5G64960 45.283 53 29 0 106 158 204 256 3.20e-11 60.8
MS.gene82944.t1 AT4G13020 39.773 88 42 2 82 159 129 215 3.52e-11 60.8
MS.gene82944.t1 AT4G13020 39.773 88 42 2 82 159 129 215 3.70e-11 60.8
MS.gene82944.t1 AT4G13020 39.773 88 42 2 82 159 138 224 4.04e-11 60.5
MS.gene82944.t1 AT4G13020 39.773 88 42 2 82 159 129 215 4.34e-11 60.5
MS.gene82944.t1 AT4G13020 49.057 53 27 0 107 159 171 223 4.77e-11 60.5
MS.gene82944.t1 AT4G13020 49.057 53 27 0 107 159 171 223 4.78e-11 60.5
MS.gene82944.t1 AT4G13020 49.057 53 27 0 107 159 163 215 5.07e-11 60.5
MS.gene82944.t1 AT5G64960 45.283 53 29 0 106 158 151 203 5.42e-11 60.5
MS.gene82944.t1 AT4G13020 49.057 53 27 0 107 159 172 224 5.46e-11 60.5

Find 39 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTTGATTTCTTCCCTTGT+TGG 0.201375 8.1:-33354261 None:intergenic
TCTTATCAGCCACTGTTTCC+TGG 0.308862 8.1:+33354035 MS.gene82944:intron
GCAGTTCTCCGATGGTTATC+TGG 0.324091 8.1:+33352518 MS.gene82944:CDS
AATGATGTCAGAGCTGAATA+AGG 0.325937 8.1:+33352584 MS.gene82944:CDS
TGGTCAGCTGGTTGCGTACT+TGG 0.374738 8.1:+33353915 MS.gene82944:CDS
AGCCTTCTGCTCACACTTGT+AGG 0.411998 8.1:-33352870 None:intergenic
TGTTATCTGCAATTTATCAT+AGG 0.415768 8.1:-33352900 None:intergenic
TACTCAGTTGCACCGAATAT+AGG 0.447929 8.1:-33353873 None:intergenic
CCTTGATAATTTCCACAAGT+TGG 0.452229 8.1:-33354073 None:intergenic
AAAGGTGAAAGCTTTCAGAT+TGG 0.471295 8.1:+33352766 MS.gene82944:intron
AGAGCTCCTGAGCCTATATT+CGG 0.474591 8.1:+33353861 MS.gene82944:CDS
GTCATTGACATCTGGTCAGC+TGG 0.482850 8.1:+33353903 MS.gene82944:CDS
TTGCAGATAACAGTGGATCA+AGG 0.505297 8.1:+33352911 MS.gene82944:CDS
CCACTGTTTCCTGGTGAGAG+TGG 0.506242 8.1:+33354044 MS.gene82944:CDS
CCAACTTGTGGAAATTATCA+AGG 0.511188 8.1:+33354073 MS.gene82944:CDS
TCCGTATGTCACGTACCTAA+AGG 0.512712 8.1:+33352623 MS.gene82944:CDS
TTGACAACAAACGTAACTTC+GGG 0.523157 8.1:-33352563 None:intergenic
ACACCACAGTCATTGACATC+TGG 0.524089 8.1:+33353895 MS.gene82944:CDS
AAAGCTTTCAGATTGGAACT+TGG 0.531075 8.1:+33352773 MS.gene82944:CDS
ATTGACAACAAACGTAACTT+CGG 0.535428 8.1:-33352564 None:intergenic
ACGCACCAAGGACCTAGAAA+AGG 0.542258 8.1:+33352799 MS.gene82944:CDS
ATGATGTCAGAGCTGAATAA+GGG 0.543037 8.1:+33352585 MS.gene82944:CDS
ACCTTTAGGTACGTGACATA+CGG 0.546444 8.1:-33352624 None:intergenic
AACCTACAAGTGTGAGCAGA+AGG 0.550765 8.1:+33352868 MS.gene82944:CDS
TGGTAAATGCAGTTCTCCGA+TGG 0.552479 8.1:+33352510 None:intergenic
GTTGCACCGAATATAGGCTC+AGG 0.565527 8.1:-33353867 None:intergenic
TGGTCTACTCCACTCTCACC+AGG 0.583110 8.1:-33354053 None:intergenic
CCAAGGACCTAGAAAAGGAA+GGG 0.587821 8.1:+33352804 MS.gene82944:CDS
ACCAAGGACCTAGAAAAGGA+AGG 0.599301 8.1:+33352803 MS.gene82944:CDS
GATCATAGATTCAGATGGTG+AGG 0.602096 8.1:+33352826 MS.gene82944:CDS
GATCAAGTCCAGATAACCAT+CGG 0.618277 8.1:-33352526 None:intergenic
GAAGGGATCATAGATTCAGA+TGG 0.634115 8.1:+33352821 MS.gene82944:CDS
TGTCAGAGCTGAATAAGGGA+GGG 0.635123 8.1:+33352589 MS.gene82944:CDS
ATGTCAGAGCTGAATAAGGG+AGG 0.639595 8.1:+33352588 MS.gene82944:CDS
CCACTCTCACCAGGAAACAG+TGG 0.653830 8.1:-33354044 None:intergenic
GGAACTTGGTACACGCACCA+AGG 0.664119 8.1:+33352787 MS.gene82944:CDS
TGATAAATTGCAGATAACAG+TGG 0.665901 8.1:+33352904 MS.gene82944:CDS
GAGTGGAGTAGACCAACTTG+TGG 0.666033 8.1:+33354061 MS.gene82944:CDS
TGACCAGATGTCAATGACTG+TGG 0.757242 8.1:-33353898 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTAGTTCAGTTAATT+CGG + chr8.1:33354202-33354221 MS.gene82944:intron 15.0%
!! AAACGAAACAAAAATATGAA+AGG - chr8.1:33354177-33354196 None:intergenic 20.0%
!! ACATCATCAAATATATTCAA+TGG + chr8.1:33353087-33353106 MS.gene82944:intron 20.0%
!! TATAAAGACTAAATGCTATA+TGG + chr8.1:33353785-33353804 MS.gene82944:intron 20.0%
!! TGAAATGATCTAATAAAACA+CGG - chr8.1:33353598-33353617 None:intergenic 20.0%
!!! ATGTTTTAGCATTTACAAAT+TGG + chr8.1:33353514-33353533 MS.gene82944:intron 20.0%
!!! TTATCATTACACTTTTTCTT+TGG - chr8.1:33353217-33353236 None:intergenic 20.0%
!!! TTTTGATGTTGTATAAAAAC+AGG + chr8.1:33353659-33353678 MS.gene82944:intron 20.0%
! ACATATCTTACATTTGTTCT+AGG + chr8.1:33353832-33353851 MS.gene82944:intron 25.0%
! CTAAACTTTCAATAACTTCA+TGG - chr8.1:33353307-33353326 None:intergenic 25.0%
! GAACAAATGTAAGATATGTT+TGG - chr8.1:33353831-33353850 None:intergenic 25.0%
! TGTTATCTGCAATTTATCAT+AGG - chr8.1:33352903-33352922 None:intergenic 25.0%
! TTGCACTTTATTCTGTAAAT+GGG + chr8.1:33353343-33353362 MS.gene82944:intron 25.0%
! TTTGCACTTTATTCTGTAAA+TGG + chr8.1:33353342-33353361 MS.gene82944:intron 25.0%
!! AAAGTATTACACAGTATATC+AGG - chr8.1:33352657-33352676 None:intergenic 25.0%
!! AGAATGGAGATAGTAATTTT+TGG + chr8.1:33353628-33353647 MS.gene82944:intron 25.0%
!! TGTATCTTGCTATAAATCAA+AGG + chr8.1:33352748-33352767 MS.gene82944:intron 25.0%
!!! AATATAGCTTTTCTGAAGTA+TGG - chr8.1:33353378-33353397 None:intergenic 25.0%
!!! AATTACCAAGAGTATTACAT+AGG - chr8.1:33352956-33352975 None:intergenic 25.0%
!!! ACTCTTGGTAATTATTTTCT+CGG + chr8.1:33352963-33352982 MS.gene82944:intron 25.0%
!!! ATTACCAAGAGTATTACATA+GGG - chr8.1:33352955-33352974 None:intergenic 25.0%
!!! GAATATATTTGATGATGTAC+AGG - chr8.1:33353085-33353104 None:intergenic 25.0%
!!! TAAATGCTATATGGTTTTAC+AGG + chr8.1:33353794-33353813 MS.gene82944:intron 25.0%
AAACTAACGAAAGGATTTCA+AGG - chr8.1:33353745-33353764 None:intergenic 30.0%
AAATTGTGCATGTCACAAAT+CGG - chr8.1:33353412-33353431 None:intergenic 30.0%
AAATTTAACCGCCAACAATT+TGG - chr8.1:33353131-33353150 None:intergenic 30.0%
ATTACTATCTCCATTCTGTT+AGG - chr8.1:33353625-33353644 None:intergenic 30.0%
ATTGACAACAAACGTAACTT+CGG - chr8.1:33352567-33352586 None:intergenic 30.0%
ATTTCACTTTCCTAACAGAA+TGG + chr8.1:33353612-33353631 MS.gene82944:intron 30.0%
GTTATTGCAAAACTAACGAA+AGG - chr8.1:33353754-33353773 None:intergenic 30.0%
TCTATTATTGCAATCTCATG+TGG - chr8.1:33353256-33353275 None:intergenic 30.0%
TGATAAATTGCAGATAACAG+TGG + chr8.1:33352904-33352923 MS.gene82944:CDS 30.0%
TGTTATGATTTCTACCAGAA+AGG + chr8.1:33353465-33353484 MS.gene82944:intron 30.0%
! CATTTCTCAATTTTCCTTTC+TGG - chr8.1:33353482-33353501 None:intergenic 30.0%
!! ATATTTGATGATGTACAGGA+AGG - chr8.1:33353081-33353100 None:intergenic 30.0%
!! CTCTTGGTAATTATTTTCTC+GGG + chr8.1:33352964-33352983 MS.gene82944:intron 30.0%
!! TTACCAAGAGTATTACATAG+GGG - chr8.1:33352954-33352973 None:intergenic 30.0%
AAAGCTTTCAGATTGGAACT+TGG + chr8.1:33352773-33352792 MS.gene82944:CDS 35.0%
AAAGGTGAAAGCTTTCAGAT+TGG + chr8.1:33352766-33352785 MS.gene82944:intron 35.0%
AAGATATGCGACTTTAGTCT+TGG + chr8.1:33353705-33353724 MS.gene82944:intron 35.0%
AATGATGTCAGAGCTGAATA+AGG + chr8.1:33352584-33352603 MS.gene82944:CDS 35.0%
ATATGTTCAAGCTAGTAGCA+AGG - chr8.1:33354141-33354160 None:intergenic 35.0%
ATGATGTCAGAGCTGAATAA+GGG + chr8.1:33352585-33352604 MS.gene82944:CDS 35.0%
CCAACTTGTGGAAATTATCA+AGG + chr8.1:33354073-33354092 MS.gene82944:CDS 35.0%
CCTTTGTAACACCAAATTGT+TGG + chr8.1:33353117-33353136 MS.gene82944:intron 35.0%
GTGACATGCACAATTTGAAT+TGG + chr8.1:33353416-33353435 MS.gene82944:intron 35.0%
GTTAATTCGGAAGTTTCATG+TGG + chr8.1:33354215-33354234 MS.gene82944:intron 35.0%
TAAAGTCGCATATCTTCAGT+TGG - chr8.1:33353701-33353720 None:intergenic 35.0%
TATCAGGAAATCACACCTTT+AGG - chr8.1:33352641-33352660 None:intergenic 35.0%
TTACCCCTATGTAATACTCT+TGG + chr8.1:33352948-33352967 MS.gene82944:intron 35.0%
TTGACAACAAACGTAACTTC+GGG - chr8.1:33352566-33352585 None:intergenic 35.0%
TTGTAACACCAAATTGTTGG+CGG + chr8.1:33353120-33353139 MS.gene82944:intron 35.0%
! CCTTGATAATTTCCACAAGT+TGG - chr8.1:33354076-33354095 None:intergenic 35.0%
!! ATTGAGCTTTGAAAGAGTCT+TGG + chr8.1:33352689-33352708 MS.gene82944:intron 35.0%
!! CCAACAATTTGGTGTTACAA+AGG - chr8.1:33353120-33353139 None:intergenic 35.0%
!! GTGCATTCGTTGATTTATGT+GGG + chr8.1:33353539-33353558 MS.gene82944:intron 35.0%
!! TGCATTCGTTGATTTATGTG+GGG + chr8.1:33353540-33353559 MS.gene82944:intron 35.0%
!!! TTTTCTTGAGTTGAGTGAGA+GGG + chr8.1:33353184-33353203 MS.gene82944:intron 35.0%
ACACAATGAAGCTAGTTACC+TGG - chr8.1:33353956-33353975 None:intergenic 40.0%
ACCTTTAGGTACGTGACATA+CGG - chr8.1:33352627-33352646 None:intergenic 40.0%
CACTTGATTTCTTCCCTTGT+TGG - chr8.1:33354264-33354283 None:intergenic 40.0%
CTAGCTTCATTGTGTATGCT+TGG + chr8.1:33353961-33353980 MS.gene82944:intron 40.0%
GAAGGGATCATAGATTCAGA+TGG + chr8.1:33352821-33352840 MS.gene82944:CDS 40.0%
GATCAAGTCCAGATAACCAT+CGG - chr8.1:33352529-33352548 None:intergenic 40.0%
GATCATAGATTCAGATGGTG+AGG + chr8.1:33352826-33352845 MS.gene82944:CDS 40.0%
TACTCAGTTGCACCGAATAT+AGG - chr8.1:33353876-33353895 None:intergenic 40.0%
TGTGTGTGTACAATATTGCG+TGG - chr8.1:33353001-33353020 None:intergenic 40.0%
TTGCAGATAACAGTGGATCA+AGG + chr8.1:33352911-33352930 MS.gene82944:CDS 40.0%
! CTATGATCCCTTCCTTTTCT+AGG - chr8.1:33352814-33352833 None:intergenic 40.0%
! CTTTGAAAGAGTCTTGGCAT+TGG + chr8.1:33352695-33352714 MS.gene82944:intron 40.0%
!! ATGGTGAGGTTTTGACAAGA+GGG + chr8.1:33352840-33352859 MS.gene82944:CDS 40.0%
!! CGTGCATTCGTTGATTTATG+TGG + chr8.1:33353538-33353557 MS.gene82944:intron 40.0%
!! GTACTTGGTGAACTTTTGCT+TGG + chr8.1:33353930-33353949 MS.gene82944:CDS 40.0%
!!! AGGTTTTAGGTACTCCAACA+AGG + chr8.1:33354247-33354266 MS.gene82944:intron 40.0%
!!! CTTTTCTGAAGTATGGATGC+AGG - chr8.1:33353371-33353390 None:intergenic 40.0%
!!! CTTTTCTTGAGTTGAGTGAG+AGG + chr8.1:33353183-33353202 MS.gene82944:intron 40.0%
!!! GGTTTTAGGTACTCCAACAA+GGG + chr8.1:33354248-33354267 MS.gene82944:intron 40.0%
AACCTACAAGTGTGAGCAGA+AGG + chr8.1:33352868-33352887 MS.gene82944:CDS 45.0%
ACACCACAGTCATTGACATC+TGG + chr8.1:33353895-33353914 MS.gene82944:CDS 45.0%
ACCAAGGACCTAGAAAAGGA+AGG + chr8.1:33352803-33352822 MS.gene82944:CDS 45.0%
AGAGCTCCTGAGCCTATATT+CGG + chr8.1:33353861-33353880 MS.gene82944:CDS 45.0%
ATGTCAGAGCTGAATAAGGG+AGG + chr8.1:33352588-33352607 MS.gene82944:CDS 45.0%
CCAAGGACCTAGAAAAGGAA+GGG + chr8.1:33352804-33352823 MS.gene82944:CDS 45.0%
GTCGCATATCTTCAGTTGGT+AGG - chr8.1:33353697-33353716 None:intergenic 45.0%
GTTTCATGTGGTTCACTTGC+AGG + chr8.1:33354227-33354246 MS.gene82944:intron 45.0%
TCCGTATGTCACGTACCTAA+AGG + chr8.1:33352623-33352642 MS.gene82944:CDS 45.0%
TCTTATCAGCCACTGTTTCC+TGG + chr8.1:33354035-33354054 MS.gene82944:intron 45.0%
TGACCAGATGTCAATGACTG+TGG - chr8.1:33353901-33353920 None:intergenic 45.0%
TGTCAGAGCTGAATAAGGGA+GGG + chr8.1:33352589-33352608 MS.gene82944:CDS 45.0%
! GTGGTTCACTTGCAGGTTTT+AGG + chr8.1:33354234-33354253 MS.gene82944:intron 45.0%
!! GATGGTGAGGTTTTGACAAG+AGG + chr8.1:33352839-33352858 MS.gene82944:CDS 45.0%
!!! TAAATTTTGAATTATTAATA+CGG + chr8.1:33353144-33353163 MS.gene82944:intron 5.0%
ACGCACCAAGGACCTAGAAA+AGG + chr8.1:33352799-33352818 MS.gene82944:CDS 50.0%
AGCCTTCTGCTCACACTTGT+AGG - chr8.1:33352873-33352892 None:intergenic 50.0%
GAGTGGAGTAGACCAACTTG+TGG + chr8.1:33354061-33354080 MS.gene82944:CDS 50.0%
GCAGTTCTCCGATGGTTATC+TGG + chr8.1:33352518-33352537 MS.gene82944:CDS 50.0%
GTCATTGACATCTGGTCAGC+TGG + chr8.1:33353903-33353922 MS.gene82944:CDS 50.0%
GTTGCACCGAATATAGGCTC+AGG - chr8.1:33353870-33353889 None:intergenic 50.0%
! CCCTTCCTTTTCTAGGTCCT+TGG - chr8.1:33352807-33352826 None:intergenic 50.0%
! TGGTGAACTTTTGCTTGGCC+AGG + chr8.1:33353935-33353954 MS.gene82944:CDS 50.0%
CCACTCTCACCAGGAAACAG+TGG - chr8.1:33354047-33354066 None:intergenic 55.0%
CCACTGTTTCCTGGTGAGAG+TGG + chr8.1:33354044-33354063 MS.gene82944:CDS 55.0%
GGAACTTGGTACACGCACCA+AGG + chr8.1:33352787-33352806 MS.gene82944:CDS 55.0%
TGGTCAGCTGGTTGCGTACT+TGG + chr8.1:33353915-33353934 MS.gene82944:CDS 55.0%
TGGTCTACTCCACTCTCACC+AGG - chr8.1:33354056-33354075 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 33352516 33354288 33352516 ID=MS.gene82944
chr8.1 mRNA 33352516 33354288 33352516 ID=MS.gene82944.t1;Parent=MS.gene82944
chr8.1 exon 33352516 33352644 33352516 ID=MS.gene82944.t1.exon1;Parent=MS.gene82944.t1
chr8.1 CDS 33352516 33352644 33352516 ID=cds.MS.gene82944.t1;Parent=MS.gene82944.t1
chr8.1 exon 33352770 33352957 33352770 ID=MS.gene82944.t1.exon2;Parent=MS.gene82944.t1
chr8.1 CDS 33352770 33352957 33352770 ID=cds.MS.gene82944.t1;Parent=MS.gene82944.t1
chr8.1 exon 33353854 33353956 33353854 ID=MS.gene82944.t1.exon3;Parent=MS.gene82944.t1
chr8.1 CDS 33353854 33353956 33353854 ID=cds.MS.gene82944.t1;Parent=MS.gene82944.t1
chr8.1 exon 33354044 33354094 33354044 ID=MS.gene82944.t1.exon4;Parent=MS.gene82944.t1
chr8.1 CDS 33354044 33354094 33354044 ID=cds.MS.gene82944.t1;Parent=MS.gene82944.t1
chr8.1 exon 33354256 33354288 33354256 ID=MS.gene82944.t1.exon5;Parent=MS.gene82944.t1
chr8.1 CDS 33354256 33354288 33354256 ID=cds.MS.gene82944.t1;Parent=MS.gene82944.t1
Gene Sequence

>MS.gene82944

ATGCAGTTCTCCGATGGTTATCTGGACTTGATCATTACGAAGAATTGCCCGAAGTTACGTTTGTTGTCAATGATGTCAGAGCTGAATAAGGGAGGGCACATGAAATCTCCGTATGTCACGTACCTAAAGGTGTGATTTCCTGATATACTGTGTAATACTTTTGTAGCAGTTACATTGAGCTTTGAAAGAGTCTTGGCATTGGTTTAGTTGCATAACGAAGTTTTCTAATTTATGTATCTTGCTATAAATCAAAGGTGAAAGCTTTCAGATTGGAACTTGGTACACGCACCAAGGACCTAGAAAAGGAAGGGATCATAGATTCAGATGGTGAGGTTTTGACAAGAGGGAGAAGAACCTACAAGTGTGAGCAGAAGGCTTTGATGACCTATGATAAATTGCAGATAACAGTGGATCAAGGTTTAGCTACACTTTTTACCCCTATGTAATACTCTTGGTAATTATTTTCTCGGGCACAATTCACACCACGCAATATTGTACACACACACACACACACACACACACACACACACACACATTCAGATACATGTTTGTATCAACAGAACCTTCCTGTACATCATCAAATATATTCAATGGCAAGTTTCCTTTGTAACACCAAATTGTTGGCGGTTAAATTTTGAATTATTAATACGGAAATTATATTATCACTCTTTTCTTGAGTTGAGTGAGAGGGTACACTTCCAAAGAAAAAGTGTAATGATAAATATTACTTGAAAAATCCACATGAGATTGCAATAATAGATGTAGTTATATACATGTGTATAAACAAACCATGAAGTTATTGAAAGTTTAGCAGTTATGTTATGATTTTGCACTTTATTCTGTAAATGGGCACCTGCATCCATACTTCAGAAAAGCTATATTCAAAAACACAACCGATTTGTGACATGCACAATTTGAATTGGTTTTGTTTGTGCTTGAATTAATTCTATGTTATGATTTCTACCAGAAAGGAAAATTGAGAAATGATTTTTTATTATATGTTTTAGCATTTACAAATTGGTCGTGCATTCGTTGATTTATGTGGGGCAGCTGTTGTTTTGCTTCTGTAGTTTTTCTTGCCGTGTTTTATTAGATCATTTCACTTTCCTAACAGAATGGAGATAGTAATTTTTGGTTGTCTCTTTTTGATGTTGTATAAAAACAGGTCAATCTTCACACCTACCAACTGAAGATATGCGACTTTAGTCTTGGTTCGTTTCTCTTAACCTTGAAATCCTTTCGTTAGTTTTGCAATAACTATCTAAGTTTTATAAAGACTAAATGCTATATGGTTTTACAGGTTAAATGTGAACCAAACATATCTTACATTTGTTCTAGGTACTATAGAGCTCCTGAGCCTATATTCGGTGCAACTGAGTACACCACAGTCATTGACATCTGGTCAGCTGGTTGCGTACTTGGTGAACTTTTGCTTGGCCAGGTAACTAGCTTCATTGTGTATGCTTGGTCAAGTTACATTTAGAATTCATGTATGTCAAAACTAATGATGAATTTCTGTTCTTATCAGCCACTGTTTCCTGGTGAGAGTGGAGTAGACCAACTTGTGGAAATTATCAAGGTAAGATATGTTTTAAACAATAATATAGCAATAGTGATATTTACCTTGCTACTAGCTTGAACATATCAGTTTTAAGTGACCTTTCATATTTTTGTTTCGTTTTGTGCTATTTTTAGTTCAGTTAATTCGGAAGTTTCATGTGGTTCACTTGCAGGTTTTAGGTACTCCAACAAGGGAAGAAATCAAGTGCATGA
Protein sequence

>MS.gene82944.t1

MQFSDGYLDLIITKNCPKLRLLSMMSELNKGGHMKSPYVTYLKVKAFRLELGTRTKDLEKEGIIDSDGEVLTRGRRTYKCEQKALMTYDKLQITVDQGLATLFTPMYYRAPEPIFGATEYTTVIDIWSAGCVLGELLLGQPLFPGESGVDQLVEIIKVLQQGKKSSA