AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82986

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82986.t1 MTR_8g071030 86.417 773 73 3 1 765 1 749 0.0 1310
MS.gene82986.t1 MTR_8g070070 74.520 781 171 8 1 765 1 769 0.0 1145
MS.gene82986.t1 MTR_8g070075 64.713 785 223 9 1 761 4 758 0.0 1009
MS.gene82986.t1 MTR_1g035420 63.197 538 183 6 229 764 134 658 0.0 697
MS.gene82986.t1 MTR_1g035400 61.702 470 177 3 288 756 101 568 0.0 608
MS.gene82986.t1 MTR_6g044690 57.419 465 175 8 298 753 114 564 0.0 538
MS.gene82986.t1 MTR_0376s0030 51.176 510 222 8 267 764 83 577 4.55e-172 507
MS.gene82986.t1 MTR_6g043960 51.509 464 183 8 298 753 53 482 1.60e-157 466
MS.gene82986.t1 MTR_2g054460 44.112 569 201 13 194 753 4 464 4.51e-144 431
MS.gene82986.t1 MTR_7g088370 43.312 471 225 17 296 753 239 680 6.41e-107 342
MS.gene82986.t1 MTR_5g013420 40.000 460 253 8 296 751 618 1058 1.98e-105 348
MS.gene82986.t1 MTR_5g013420 40.000 460 253 8 296 751 618 1058 2.17e-105 348
MS.gene82986.t1 MTR_3g011440 36.315 559 321 13 204 752 100 633 1.47e-104 335
MS.gene82986.t1 MTR_4g010830 40.741 459 250 9 296 751 510 949 3.80e-102 337
MS.gene82986.t1 MTR_8g042750 40.381 473 249 13 296 753 204 658 9.57e-102 328
MS.gene82986.t1 MTR_4g010550 38.961 462 224 12 296 751 24 433 1.49e-93 300
MS.gene82986.t1 MTR_7g084090 37.967 482 259 14 296 752 261 727 1.20e-87 293
MS.gene82986.t1 MTR_6g061270 37.838 481 260 13 296 752 199 664 2.23e-86 288
MS.gene82986.t1 MTR_6g061200 37.421 473 244 15 296 752 199 635 1.27e-80 271
MS.gene82986.t1 MTR_7g084090 41.322 363 183 11 296 641 261 610 3.50e-72 249
MS.gene82986.t1 MTR_7g445710 31.875 480 228 17 295 751 109 512 5.23e-54 196
MS.gene82986.t1 MTR_7g445530 31.667 480 229 17 295 751 109 512 7.28e-54 194
MS.gene82986.t1 MTR_1g048950 32.979 470 264 15 298 751 73 507 4.36e-52 191
MS.gene82986.t1 MTR_7g450730 30.349 458 227 15 296 751 107 474 7.33e-51 186
MS.gene82986.t1 MTR_7g052480 31.613 310 186 9 459 752 227 526 1.13e-38 152
MS.gene82986.t1 MTR_7g445600 34.667 150 61 2 602 751 243 355 1.33e-22 100
MS.gene82986.t1 MTR_7g445600 37.984 129 66 5 296 424 106 220 4.64e-15 77.8
MS.gene82986.t1 MTR_6g044000 67.925 53 17 0 701 753 117 169 1.21e-20 90.1
MS.gene82986.t1 MTR_6g044000 52.941 68 32 0 298 365 53 120 2.47e-18 83.6
MS.gene82986.t1 MTR_8g078490 29.730 185 118 3 568 751 1056 1229 3.72e-20 96.7
MS.gene82986.t1 MTR_5g018850 31.492 181 112 3 568 747 1310 1479 4.26e-20 96.7
MS.gene82986.t1 MTR_8g078505 27.857 280 155 8 503 751 252 515 6.41e-20 94.7
MS.gene82986.t1 MTR_4g132610 29.101 189 112 3 569 751 851 1023 6.27e-19 92.4
MS.gene82986.t1 MTR_5g098680 36.076 158 87 4 321 473 267 415 4.90e-16 82.8
MS.gene82986.t1 MTR_8g102580 36.076 158 87 4 321 473 261 409 1.04e-15 81.6
MS.gene82986.t1 MTR_7g445810 28.287 251 130 10 226 450 38 264 6.28e-13 70.5
MS.gene82986.t1 MTR_3g114910 33.333 153 88 4 321 473 187 325 1.40e-12 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82986.t1 AT4G13460 61.237 485 181 5 282 764 170 649 0.0 629
MS.gene82986.t1 AT4G13460 61.237 485 181 5 282 764 170 649 0.0 629
MS.gene82986.t1 AT2G33290 58.264 484 190 5 282 764 178 650 0.0 602
MS.gene82986.t1 AT2G33290 58.264 484 190 5 282 764 178 650 0.0 602
MS.gene82986.t1 AT2G33290 58.264 484 190 5 282 764 178 650 0.0 602
MS.gene82986.t1 AT2G33290 58.264 484 190 5 282 764 178 650 0.0 602
MS.gene82986.t1 AT5G04940 40.166 483 259 15 279 753 180 640 2.54e-98 319
MS.gene82986.t1 AT5G04940 40.166 483 259 15 279 753 180 640 2.54e-98 319
MS.gene82986.t1 AT1G73100 36.347 553 312 15 220 753 108 639 8.98e-96 312
MS.gene82986.t1 AT2G22740 36.820 478 275 8 285 751 298 759 4.45e-93 308
MS.gene82986.t1 AT2G22740 36.820 478 275 8 285 751 298 759 4.45e-93 308
MS.gene82986.t1 AT2G35160 34.857 525 291 13 236 751 245 727 8.18e-90 298
MS.gene82986.t1 AT2G35160 34.857 525 291 13 236 751 281 763 3.00e-89 298
MS.gene82986.t1 AT2G35160 34.857 525 291 13 236 751 281 763 3.00e-89 298
MS.gene82986.t1 AT2G35160 34.857 525 291 13 236 751 281 763 3.00e-89 298
MS.gene82986.t1 AT2G35160 34.857 525 291 13 236 751 281 763 3.00e-89 298
MS.gene82986.t1 AT5G13960 36.831 486 258 16 296 752 129 594 1.20e-83 278
MS.gene82986.t1 AT5G13960 37.313 469 246 15 313 752 11 460 5.03e-83 273
MS.gene82986.t1 AT1G17770 31.836 512 314 16 258 753 169 661 6.26e-72 249
MS.gene82986.t1 AT2G24740 36.078 510 285 18 253 753 247 724 6.32e-72 250
MS.gene82986.t1 AT5G47150 40.449 178 97 4 296 473 156 324 7.37e-28 115
MS.gene82986.t1 AT5G47160 40.351 171 92 5 300 469 246 407 3.25e-24 106
MS.gene82986.t1 AT5G47160 40.351 171 92 5 300 469 246 407 3.25e-24 106
MS.gene82986.t1 AT5G47160 40.351 171 92 5 300 469 246 407 3.25e-24 106
MS.gene82986.t1 AT2G23740 30.964 197 109 5 570 756 1184 1363 5.34e-22 102
MS.gene82986.t1 AT2G23740 30.964 197 109 5 570 756 1184 1363 5.34e-22 102
MS.gene82986.t1 AT2G23740 30.964 197 109 5 570 756 1184 1363 5.34e-22 102
MS.gene82986.t1 AT2G23740 30.964 197 109 5 570 756 1177 1356 5.42e-22 102
MS.gene82986.t1 AT2G05900 38.506 174 99 6 358 531 26 191 1.28e-19 90.9
MS.gene82986.t1 AT1G57820 36.047 172 86 6 317 473 264 426 1.21e-16 84.7
MS.gene82986.t1 AT1G57820 36.047 172 86 6 317 473 264 426 1.21e-16 84.7
MS.gene82986.t1 AT3G03750 30.918 207 115 6 548 752 143 323 3.45e-16 81.3
MS.gene82986.t1 AT1G57820 35.503 169 88 6 317 473 264 423 1.52e-15 81.3
MS.gene82986.t1 AT5G39550 34.302 172 89 6 317 473 249 411 1.73e-15 80.9
MS.gene82986.t1 AT2G23740 30.405 148 79 4 570 710 1184 1314 5.16e-15 80.1
MS.gene82986.t1 AT2G23740 30.405 148 79 4 570 710 1184 1314 5.16e-15 80.1
MS.gene82986.t1 AT1G66040 31.977 172 93 5 317 473 249 411 1.07e-14 78.6
MS.gene82986.t1 AT1G66050 31.977 172 93 5 317 473 249 411 1.12e-14 78.2

Find 231 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAAATCTCAACCTTAATT+TGG 0.115237 8.3:+31485624 MS.gene82986:CDS
GCTAGGGATATTAAGAAAAT+TGG 0.127502 8.3:+31486660 MS.gene82986:CDS
CCAAATTAAGGTTGAGATTT+AGG 0.131281 8.3:-31485624 None:intergenic
GCTACTAGTGTAATTGTTTC+AGG 0.143034 8.3:+31486294 MS.gene82986:CDS
CCCCATTCTACCCATCTATT+TGG 0.166114 8.3:-31487203 None:intergenic
CTATTGATATTTCAAATAAT+AGG 0.206413 8.3:+31486712 MS.gene82986:CDS
GCAAATTTGTGATCAGAAAT+TGG 0.219259 8.3:+31486401 MS.gene82986:CDS
ACATACTGAATGAATATATT+TGG 0.228005 8.3:-31487350 None:intergenic
GATGGATATGGTGGTGATTT+GGG 0.254601 8.3:-31485365 None:intergenic
AAGCAATCCATGTGGATTAT+AGG 0.255997 8.3:-31486922 None:intergenic
TAGGTCCTTGGACCTTATTC+AGG 0.261644 8.3:+31487079 MS.gene82986:CDS
CGATGGATATGGTGGTGATT+TGG 0.266979 8.3:-31485366 None:intergenic
CTAAATCTCAACCTTAATTT+GGG 0.267648 8.3:+31485625 MS.gene82986:CDS
GGTGAGAGCGGTGTGATTTA+AGG 0.267982 8.3:-31485338 None:intergenic
TCAGATTCGGGAGAGAAATT+AGG 0.279456 8.3:-31485469 None:intergenic
TGATTTAAGGTTGAAATTCT+AGG 0.282052 8.3:-31485325 None:intergenic
TGAGAGTGGGTGAGGAGAAT+AGG 0.282380 8.3:+31485998 MS.gene82986:CDS
TAGGTTGTTGTTGGGAATCT+TGG 0.291847 8.3:-31485450 None:intergenic
GAGAGTGGGTGAGGAGAATA+GGG 0.304281 8.3:+31485999 MS.gene82986:CDS
AATTGGTTGTTGAAGTTGAT+AGG 0.309699 8.3:+31485890 MS.gene82986:CDS
TATTGATATTTCAAATAATA+GGG 0.311494 8.3:+31486713 MS.gene82986:CDS
TGATCATCGGTTTCAGATTC+GGG 0.313526 8.3:-31485481 None:intergenic
GCAACACGGTTTCGACAATT+AGG 0.314745 8.3:-31486996 None:intergenic
GATATAGGAAAGTCTGGTTT+TGG 0.315495 8.3:+31486570 MS.gene82986:CDS
GTCGAGTTTGATGGCATATA+TGG 0.315529 8.3:+31485708 MS.gene82986:CDS
GAATGGGTTTCAAGGGTTAA+TGG 0.316851 8.3:-31485223 None:intergenic
TTGATCATCGGTTTCAGATT+CGG 0.324605 8.3:-31485482 None:intergenic
AATATAGCTTGGAAGTGTTT+AGG 0.327076 8.3:+31487033 MS.gene82986:CDS
AATGAATGAGGAGAAGAAAT+TGG 0.327732 8.3:+31485873 MS.gene82986:CDS
AGCAATCCATGTGGATTATA+GGG 0.332580 8.3:-31486921 None:intergenic
GACGCGAAGAAATTGTTAAA+AGG 0.332988 8.3:-31485427 None:intergenic
ATATATTTGGACTCGAACTA+TGG 0.334818 8.3:-31487337 None:intergenic
ATCTTGCTCGCCCACTCGTC+CGG 0.340319 8.3:-31487470 None:intergenic
TTAGGAGGACATGAGCAAAA+AGG 0.343616 8.3:-31486978 None:intergenic
AGAGAACTCTGCATAGATTA+TGG 0.345054 8.3:+31487443 MS.gene82986:CDS
AATGGGTTTCAAGGGTTAAT+GGG 0.348241 8.3:-31485222 None:intergenic
GGGAGACAATTAATGGAATA+AGG 0.348461 8.3:+31486043 MS.gene82986:CDS
ATATCATTAAATAGCCTAAC+TGG 0.350719 8.3:-31486744 None:intergenic
GTTAGTTGCTTGTTTGATAT+AGG 0.351027 8.3:+31486555 MS.gene82986:CDS
AGAAATTGTTAAAAGGTTCC+AGG 0.355471 8.3:-31485420 None:intergenic
CACAGACAGGTTGGGGAGTT+AGG 0.355710 8.3:+31487060 MS.gene82986:CDS
ATTGGATGAGGAGAAGATAT+TGG 0.356889 8.3:+31485945 MS.gene82986:CDS
CTAGTACATTTCCAAATAGA+TGG 0.359463 8.3:+31487192 MS.gene82986:CDS
GAAGGGAGTGCATCTACTTC+TGG 0.362995 8.3:+31486498 MS.gene82986:CDS
TAAAGGATCCAGCCTGAATA+AGG 0.368631 8.3:-31487091 None:intergenic
TTTGATGGCATATATGGTTA+TGG 0.371504 8.3:+31485714 MS.gene82986:CDS
ATTGTCTGAGGAAAGGAAAT+TGG 0.371576 8.3:+31485927 MS.gene82986:CDS
AAGTGATGTTCCGATGGATA+TGG 0.384008 8.3:-31485377 None:intergenic
ACCAGTATACTCGCAGATAA+AGG 0.385474 8.3:-31487108 None:intergenic
TATGGTGGTGATTTGGGGTG+GGG 0.386266 8.3:-31485359 None:intergenic
TTGTTAAAAGGTTCCAGGTT+TGG 0.388510 8.3:-31485415 None:intergenic
CGTAGTATCAACCACATTCA+TGG 0.388913 8.3:+31486069 MS.gene82986:CDS
CTAGGGATATTAAGAAAATT+GGG 0.394850 8.3:+31486661 MS.gene82986:CDS
TCGAAACCGTGTTGCACAAA+AGG 0.395320 8.3:+31487004 MS.gene82986:CDS
TGTTGTTTCATTGGAGAATA+CGG 0.399431 8.3:+31485582 MS.gene82986:CDS
TCCAAATAGATGGGTAGAAT+GGG 0.401426 8.3:+31487202 MS.gene82986:CDS
GAGAGAAATTAGGTTGTTGT+TGG 0.401584 8.3:-31485459 None:intergenic
TGCACTATGGGATTGAGGTT+AGG 0.405470 8.3:+31486454 MS.gene82986:CDS
ACCACATTCATGGTTATATG+AGG 0.405787 8.3:+31486079 MS.gene82986:CDS
TCAAAGAGTATGTCTTATAA+TGG 0.405858 8.3:+31486261 MS.gene82986:CDS
GATATGGTGGTGATTTGGGG+TGG 0.408728 8.3:-31485361 None:intergenic
TAGAATTTGGCGTATAGGTT+GGG 0.409698 8.3:-31485505 None:intergenic
TGTGGTAGAATTTGGCGTAT+AGG 0.411852 8.3:-31485510 None:intergenic
AGAAATAGAAGTAGAAGAAT+GGG 0.413089 8.3:-31485239 None:intergenic
GCACTATGGGATTGAGGTTA+GGG 0.416487 8.3:+31486455 MS.gene82986:CDS
TTCCAAATAGATGGGTAGAA+TGG 0.421060 8.3:+31487201 MS.gene82986:CDS
TTGTTTGATATAGGAAAGTC+TGG 0.421956 8.3:+31486564 MS.gene82986:CDS
TAGGTCTACACAGACAGGTT+GGG 0.422135 8.3:+31487052 MS.gene82986:CDS
GAGAAATAGAAGTAGAAGAA+TGG 0.422660 8.3:-31485240 None:intergenic
TGAAACAACAAAATCATTGT+TGG 0.424236 8.3:-31485569 None:intergenic
GGCAGAGATGAGAGTGGGTG+AGG 0.425042 8.3:+31485990 MS.gene82986:CDS
TCCTTTATCTGCGAGTATAC+TGG 0.426481 8.3:+31487107 MS.gene82986:CDS
AGGTTGTTGTTGGGAATCTT+GGG 0.427326 8.3:-31485449 None:intergenic
CATCTATTTGGAAATGTACT+AGG 0.428903 8.3:-31487191 None:intergenic
GAAACAAGAGTCGAGTTTGA+TGG 0.434269 8.3:+31485699 MS.gene82986:CDS
AAGTAGAAGAATGGGTTTCA+AGG 0.435832 8.3:-31485231 None:intergenic
TATAAGTTATTGAGAATTGA+GGG 0.436671 8.3:+31486603 MS.gene82986:CDS
GTAGAATTTGGCGTATAGGT+TGG 0.436679 8.3:-31485506 None:intergenic
TCTTTATTATGAGTATCTTC+CGG 0.438277 8.3:+31486785 MS.gene82986:CDS
ATTGGTTGTTGAAGTTGATA+GGG 0.446123 8.3:+31485891 MS.gene82986:CDS
TCCTTGGACCTTATTCAGGC+TGG 0.446886 8.3:+31487083 MS.gene82986:CDS
CGAAACCGTGTTGCACAAAA+GGG 0.448108 8.3:+31487005 MS.gene82986:CDS
AATTGAGGGGCAGGCGAAGA+TGG 0.448823 8.3:+31486617 MS.gene82986:CDS
AAACTCGACTCTTGTTTCTC+AGG 0.449003 8.3:-31485694 None:intergenic
AGGAGGGAATTGTGTGTGAC+TGG 0.450008 8.3:+31486201 MS.gene82986:CDS
ACCTAGTGGTTGTGAATGTA+AGG 0.454638 8.3:+31486848 MS.gene82986:CDS
GGAATAGGAACATGAGAAGA+AGG 0.457689 8.3:-31485265 None:intergenic
AGAGAAATTAGGTTGTTGTT+GGG 0.464968 8.3:-31485458 None:intergenic
ATATGGTGGTGATTTGGGGT+GGG 0.466003 8.3:-31485360 None:intergenic
TTGATGAGAAATACTAAGAT+CGG 0.467844 8.3:-31485299 None:intergenic
GATTTAAGGTTGAAATTCTA+GGG 0.470586 8.3:-31485324 None:intergenic
TAAGGTTGAGATTTAGGTCC+GGG 0.474895 8.3:-31485618 None:intergenic
TTGGAAAGGAGTATGCACTA+TGG 0.475190 8.3:+31486441 MS.gene82986:CDS
ACATCACCAACATAAATTCC+AGG 0.479414 8.3:-31486168 None:intergenic
TGATGAGAAATACTAAGATC+GGG 0.480139 8.3:-31485298 None:intergenic
GGTTTGTGTTTCATCAGAGT+GGG 0.481554 8.3:+31486823 MS.gene82986:CDS
AGTAGAAGAATGGGTTTCAA+GGG 0.482906 8.3:-31485230 None:intergenic
GGATATTAAGAAAATTGGGT+TGG 0.483689 8.3:+31486665 MS.gene82986:CDS
AGGATAGTTGGACCGATACC+TGG 0.485997 8.3:+31486150 MS.gene82986:CDS
TTCGCTTCGCGTGAGAATTG+AGG 0.488587 8.3:+31485840 MS.gene82986:CDS
TCTTGCTCGCCCACTCGTCC+GGG 0.490402 8.3:-31487469 None:intergenic
GGTTATGGAGAAGGAAAGCC+AGG 0.494082 8.3:+31485729 MS.gene82986:CDS
AATTGTTTCAGGTGGTTATG+AGG 0.495448 8.3:+31486305 MS.gene82986:CDS
TAATCTAAAGGAGGTATTGA+CGG 0.498715 8.3:-31487269 None:intergenic
ATAGGGAAAATGTTCCAGTT+AGG 0.499113 8.3:+31486730 MS.gene82986:CDS
TATCGTGATCAAAGGATAGT+TGG 0.501111 8.3:+31486138 MS.gene82986:CDS
GTATAAGTTATTGAGAATTG+AGG 0.501728 8.3:+31486602 MS.gene82986:CDS
CACTGTTCTCAAAGATGCTA+GGG 0.502270 8.3:+31486644 MS.gene82986:CDS
CCCTCACAAGTTCCATCACC+TGG 0.503770 8.3:-31485747 None:intergenic
TCATGTCATGTTATATGCAA+TGG 0.506033 8.3:+31487403 MS.gene82986:CDS
TCTATGCAGAGTTCTCTCAT+AGG 0.506224 8.3:-31487437 None:intergenic
GTCGGCTACCAACGTGGAGG+GGG 0.510887 8.3:+31486023 MS.gene82986:CDS
AAAGGGGCTCAAATATAGCT+TGG 0.511664 8.3:+31487022 MS.gene82986:CDS
GAATCAGCGATTTCGAGAAA+AGG 0.512004 8.3:-31485547 None:intergenic
AGGTCTACACAGACAGGTTG+GGG 0.513098 8.3:+31487053 MS.gene82986:CDS
GATTTGGGGTGGGGTGAGAG+CGG 0.514925 8.3:-31485350 None:intergenic
GGGGTGGATTCTGTAAATGA+TGG 0.515279 8.3:+31485646 MS.gene82986:CDS
ACTCGAACTATGGCTCATAT+AGG 0.515301 8.3:-31487327 None:intergenic
TTAAGGTTGAGATTTAGGTC+CGG 0.515596 8.3:-31485619 None:intergenic
GAGTGCATCTACTTCTGGTA+AGG 0.518621 8.3:+31486503 MS.gene82986:CDS
GTATAGGTTGGGTTGATCAT+CGG 0.521132 8.3:-31485494 None:intergenic
TCAATAGAGATACAACTCAT+TGG 0.521177 8.3:-31486696 None:intergenic
TGCACTTTCGTCATAAGATA+AGG 0.521451 8.3:+31485797 MS.gene82986:CDS
GTGTCGGCTACCAACGTGGA+GGG 0.521469 8.3:+31486021 MS.gene82986:CDS
GCACTGTTCTCAAAGATGCT+AGG 0.522783 8.3:+31486643 MS.gene82986:CDS
TGTCGGCTACCAACGTGGAG+GGG 0.522877 8.3:+31486022 MS.gene82986:CDS
GCATACTCCTTTCCAATGCA+AGG 0.525232 8.3:-31486434 None:intergenic
GGCATATATGGTTATGGAGA+AGG 0.527217 8.3:+31485720 MS.gene82986:CDS
GGAGGTATTGACGGATAAGA+TGG 0.528649 8.3:-31487260 None:intergenic
TAATGAAGGAGCGTGGGCTA+TGG 0.529339 8.3:+31486112 MS.gene82986:CDS
AATCAGCGATTTCGAGAAAA+GGG 0.530354 8.3:-31485546 None:intergenic
AGAAGAGATTGTCTGAGGAA+AGG 0.532258 8.3:+31485920 MS.gene82986:CDS
GGAGAAGGAAAGCCAGGTGA+TGG 0.532924 8.3:+31485735 MS.gene82986:CDS
TGGTTTGTGTTTCATCAGAG+TGG 0.533225 8.3:+31486822 MS.gene82986:CDS
ATTATAATCTACACTGGCCA+TGG 0.534081 8.3:+31486354 MS.gene82986:CDS
TACTGGTGTTGCTATAACTA+GGG 0.536645 8.3:+31487124 MS.gene82986:CDS
AACAATGAATGGGGTCTCGT+TGG 0.538408 8.3:+31487163 MS.gene82986:CDS
GTTATTGAGAATTGAGGGGC+AGG 0.539794 8.3:+31486608 MS.gene82986:CDS
ACAATTACACTAGTAGCAAT+CGG 0.540891 8.3:-31486288 None:intergenic
TGGAAAGGAGTATGCACTAT+GGG 0.541585 8.3:+31486442 MS.gene82986:CDS
AATTCTTCTCTCGCCCTCCA+TGG 0.544307 8.3:-31486371 None:intergenic
GGTTGTTGAAGTTGATAGGG+AGG 0.545333 8.3:+31485894 MS.gene82986:CDS
ACATCCAGAAAATAATCTAA+AGG 0.546485 8.3:-31487281 None:intergenic
CTGATCACAAATTTGCCTTG+AGG 0.547153 8.3:-31486394 None:intergenic
ATTGAGGGGCAGGCGAAGAT+GGG 0.548419 8.3:+31486618 MS.gene82986:CDS
ATACTGGTGTTGCTATAACT+AGG 0.549242 8.3:+31487123 MS.gene82986:CDS
AACGCCACTCATTCCAAACC+TGG 0.552250 8.3:+31485402 MS.gene82986:CDS
GAGTATGCACTATGGGATTG+AGG 0.552471 8.3:+31486449 MS.gene82986:CDS
CCAGGTGATGGAACTTGTGA+GGG 0.555665 8.3:+31485747 MS.gene82986:CDS
ATGCAGAGTTCTCTCATAGG+AGG 0.558353 8.3:-31487434 None:intergenic
GGAGGGTGGAAACCTTGCAT+TGG 0.558530 8.3:+31486422 MS.gene82986:CDS
CTCTGATGAAACACAAACCA+CGG 0.558774 8.3:-31486819 None:intergenic
GGAAGATACTCATAATAAAG+AGG 0.560347 8.3:-31486783 None:intergenic
GGTAAGGTTTATGTTTACGA+TGG 0.560574 8.3:+31486519 MS.gene82986:CDS
GTTAATGGGAAAGGATCCAT+TGG 0.561622 8.3:-31485208 None:intergenic
TGAGGAAAGGAAATTGGATG+AGG 0.567529 8.3:+31485933 MS.gene82986:CDS
ATTCGCGGGATGAAGTATGA+AGG 0.568414 8.3:+31486480 MS.gene82986:CDS
GGAGGACAAGAGAATGAATG+AGG 0.571585 8.3:+31485861 MS.gene82986:CDS
GGAAGAGAAGAGATTGTCTG+AGG 0.572868 8.3:+31485915 MS.gene82986:CDS
AACCACGGGAAAGTAGTATC+CGG 0.574690 8.3:-31486804 None:intergenic
CCGATACCTGGAATTTATGT+TGG 0.577931 8.3:+31486162 MS.gene82986:CDS
ATTGAGGTTAGGGTTATTCG+CGG 0.577972 8.3:+31486465 MS.gene82986:CDS
GGTTCCAGGTTTGGAATGAG+TGG 0.578526 8.3:-31485406 None:intergenic
GGTTTCAGATCTGAGTATGA+AGG 0.579176 8.3:+31485768 MS.gene82986:CDS
GTGGAAACCTTGCATTGGAA+AGG 0.581268 8.3:+31486427 MS.gene82986:CDS
ATGGATATGGTGGTGATTTG+GGG 0.583200 8.3:-31485364 None:intergenic
CGACATTAATGAAGGAGCGT+GGG 0.584881 8.3:+31486106 MS.gene82986:CDS
TAGTACATTTCCAAATAGAT+GGG 0.585798 8.3:+31487193 MS.gene82986:CDS
GAGGGCTGCGACATTAATGA+AGG 0.586703 8.3:+31486098 MS.gene82986:CDS
CTGCATAGATTATGGCATCC+CGG 0.587677 8.3:+31487451 MS.gene82986:CDS
GGATGCAGAAGTTGCAGTTG+TGG 0.588062 8.3:+31485966 MS.gene82986:CDS
CCAACATAAATTCCAGGTAT+CGG 0.588388 8.3:-31486162 None:intergenic
TAAATCTCAACCTTAATTTG+GGG 0.590545 8.3:+31485626 MS.gene82986:CDS
GTGTTTAGGTCTACACAGAC+AGG 0.591190 8.3:+31487047 MS.gene82986:CDS
TATGGTTGTATCGTGATCAA+AGG 0.592056 8.3:+31486130 MS.gene82986:CDS
TGTGATCAGAAATTGGAGGG+TGG 0.592511 8.3:+31486408 MS.gene82986:CDS
GTTTCAAGGGTTAATGGGAA+AGG 0.597617 8.3:-31485217 None:intergenic
GCCAGGTGATGGAACTTGTG+AGG 0.598287 8.3:+31485746 MS.gene82986:CDS
TGCAGAGTTCTCTCATAGGA+GGG 0.599457 8.3:-31487433 None:intergenic
TAATCTACACTGGCCATGGA+GGG 0.600090 8.3:+31486358 MS.gene82986:CDS
ACACGGTTTCGACAATTAGG+AGG 0.600465 8.3:-31486993 None:intergenic
ACTAGTGTAATTGTTTCAGG+TGG 0.601921 8.3:+31486297 MS.gene82986:CDS
TACGGTAAATGTTTGCAACC+CGG 0.602140 8.3:+31485600 MS.gene82986:CDS
AGGTTGGGGAGTTAGGTCCT+TGG 0.602845 8.3:+31487067 MS.gene82986:CDS
AGGTGGTTATGAGGATGACA+TGG 0.604358 8.3:+31486314 MS.gene82986:CDS
GGTGATATTATAATCTACAC+TGG 0.606082 8.3:+31486348 MS.gene82986:CDS
GTGGAGGCAGAGATGAGAGT+GGG 0.608465 8.3:+31485985 MS.gene82986:CDS
CCACATTCATGGTTATATGA+GGG 0.608512 8.3:+31486080 MS.gene82986:CDS
TTCGTCATAAGATAAGGAAG+AGG 0.608918 8.3:+31485803 MS.gene82986:CDS
ATTGCATATAACATGACATG+AGG 0.609949 8.3:-31487401 None:intergenic
GCGACATTAATGAAGGAGCG+TGG 0.612288 8.3:+31486105 MS.gene82986:CDS
ATTATGGCATCCCGGACGAG+TGG 0.613316 8.3:+31487459 MS.gene82986:CDS
TCTGATGAAACACAAACCAC+GGG 0.613935 8.3:-31486818 None:intergenic
ATTTGTGATCAGAAATTGGA+GGG 0.614502 8.3:+31486405 MS.gene82986:CDS
TTGCATATGCATATGCAAGC+TGG 0.615698 8.3:+31486225 MS.gene82986:CDS
CGTTTGAAGTGATGTTCCGA+TGG 0.617924 8.3:-31485383 None:intergenic
GTTTGTGTTTCATCAGAGTG+GGG 0.619286 8.3:+31486824 MS.gene82986:CDS
GAGTTTCCCTATAATCCACA+TGG 0.620559 8.3:+31486915 MS.gene82986:CDS
CCCTCATATAACCATGAATG+TGG 0.621384 8.3:-31486080 None:intergenic
GGGTGAGGAGAATAGGGTGT+CGG 0.621767 8.3:+31486005 MS.gene82986:CDS
TCCAGCCTGAATAAGGTCCA+AGG 0.622985 8.3:-31487084 None:intergenic
ATAAGTTATTGAGAATTGAG+GGG 0.623555 8.3:+31486604 MS.gene82986:CDS
TTCGCGGGATGAAGTATGAA+GGG 0.624136 8.3:+31486481 MS.gene82986:CDS
TTATGGCATCCCGGACGAGT+GGG 0.624731 8.3:+31487460 MS.gene82986:CDS
CAAATCACCACCATATCCAT+CGG 0.625118 8.3:+31485367 MS.gene82986:CDS
ATAATCTACACTGGCCATGG+AGG 0.626398 8.3:+31486357 MS.gene82986:CDS
TTGAGGTTAGGGTTATTCGC+GGG 0.628964 8.3:+31486466 MS.gene82986:CDS
GGTGTCGGCTACCAACGTGG+AGG 0.632053 8.3:+31486020 MS.gene82986:CDS
TCCAGAAAATAATCTAAAGG+AGG 0.632885 8.3:-31487278 None:intergenic
TTAGGTCTACACAGACAGGT+TGG 0.633420 8.3:+31487051 MS.gene82986:CDS
TGTAAGGAGGCTTGTACTGA+CGG 0.637592 8.3:+31486864 MS.gene82986:CDS
TGCAGAAGTTGCAGTTGTGG+AGG 0.639345 8.3:+31485969 MS.gene82986:CDS
GATGAGAAATACTAAGATCG+GGG 0.640700 8.3:-31485297 None:intergenic
CCTTTCAAAAGCAATCCATG+TGG 0.644916 8.3:-31486930 None:intergenic
GCTTCGCGTGAGAATTGAGG+AGG 0.645275 8.3:+31485843 MS.gene82986:CDS
AAATCTCAACCTTAATTTGG+GGG 0.645557 8.3:+31485627 MS.gene82986:CDS
TTCCGGATACTACTTTCCCG+TGG 0.649132 8.3:+31486802 MS.gene82986:CDS
CCAAATAGATGGGTAGAATG+GGG 0.657480 8.3:+31487203 MS.gene82986:CDS
TCCTTACATTCACAACCACT+AGG 0.658950 8.3:-31486849 None:intergenic
CATCAGAGTGGGGAACCTAG+TGG 0.659288 8.3:+31486834 MS.gene82986:CDS
TTGCATATAACATGACATGA+GGG 0.662482 8.3:-31487400 None:intergenic
GGCGAGAGAAGAATTCCTCA+AGG 0.664502 8.3:+31486379 MS.gene82986:CDS
GAAACCGTGTTGCACAAAAG+GGG 0.665958 8.3:+31487006 MS.gene82986:CDS
TGATGTTCCGATGGATATGG+TGG 0.669643 8.3:-31485374 None:intergenic
ATGAGAAATACTAAGATCGG+GGG 0.677362 8.3:-31485296 None:intergenic
TAGTGGTTGTGAATGTAAGG+AGG 0.682180 8.3:+31486851 MS.gene82986:CDS
TAGGGTGTCGGCTACCAACG+TGG 0.694009 8.3:+31486017 MS.gene82986:CDS
AATTTGTGATCAGAAATTGG+AGG 0.699714 8.3:+31486404 MS.gene82986:CDS
TGTGGAGGCAGAGATGAGAG+TGG 0.704475 8.3:+31485984 MS.gene82986:CDS
AAATGATCGAGCTATTGTTG+TGG 0.743063 8.3:+31485669 MS.gene82986:CDS
GATGACATGGAATTAGACGA+TGG 0.783210 8.3:+31486327 MS.gene82986:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGATATTTCAAATAATA+GGG + chr8.3:31486713-31486732 MS.gene82986:CDS 10.0%
!! CTATTGATATTTCAAATAAT+AGG + chr8.3:31486712-31486731 MS.gene82986:CDS 15.0%
!! ACATACTGAATGAATATATT+TGG - chr8.3:31487353-31487372 None:intergenic 20.0%
!! TATAAGTTATTGAGAATTGA+GGG + chr8.3:31486603-31486622 MS.gene82986:CDS 20.0%
!!! CTCAAATTTTAACAATGAAT+GGG + chr8.3:31487153-31487172 MS.gene82986:CDS 20.0%
!!! TCAAATTTTAACAATGAATG+GGG + chr8.3:31487154-31487173 MS.gene82986:CDS 20.0%
! ACATCCAGAAAATAATCTAA+AGG - chr8.3:31487284-31487303 None:intergenic 25.0%
! AGAAATAGAAGTAGAAGAAT+GGG - chr8.3:31485242-31485261 None:intergenic 25.0%
! ATAAGTTATTGAGAATTGAG+GGG + chr8.3:31486604-31486623 MS.gene82986:CDS 25.0%
! ATATCATTAAATAGCCTAAC+TGG - chr8.3:31486747-31486766 None:intergenic 25.0%
! CTAAATCTCAACCTTAATTT+GGG + chr8.3:31485625-31485644 MS.gene82986:CDS 25.0%
! CTAGGGATATTAAGAAAATT+GGG + chr8.3:31486661-31486680 MS.gene82986:CDS 25.0%
! GTATAAGTTATTGAGAATTG+AGG + chr8.3:31486602-31486621 MS.gene82986:CDS 25.0%
! TAGTACATTTCCAAATAGAT+GGG + chr8.3:31487193-31487212 MS.gene82986:CDS 25.0%
! TCTTTATTATGAGTATCTTC+CGG + chr8.3:31486785-31486804 MS.gene82986:CDS 25.0%
! TGAAACAACAAAATCATTGT+TGG - chr8.3:31485572-31485591 None:intergenic 25.0%
! TTGATGAGAAATACTAAGAT+CGG - chr8.3:31485302-31485321 None:intergenic 25.0%
!! GATTTAAGGTTGAAATTCTA+GGG - chr8.3:31485327-31485346 None:intergenic 25.0%
!! TAAATCTCAACCTTAATTTG+GGG + chr8.3:31485626-31485645 MS.gene82986:CDS 25.0%
!! TCAAAGAGTATGTCTTATAA+TGG + chr8.3:31486261-31486280 MS.gene82986:CDS 25.0%
!! TGATTTAAGGTTGAAATTCT+AGG - chr8.3:31485328-31485347 None:intergenic 25.0%
!!! AAACCTTTGATTTTTGAATG+CGG + chr8.3:31486954-31486973 MS.gene82986:CDS 25.0%
!!! CAATGATTTTGTTGTTTCAT+TGG + chr8.3:31485573-31485592 MS.gene82986:CDS 25.0%
!!! GCTCAAATTTTAACAATGAA+TGG + chr8.3:31487152-31487171 MS.gene82986:CDS 25.0%
AATGAATGAGGAGAAGAAAT+TGG + chr8.3:31485873-31485892 MS.gene82986:CDS 30.0%
AATTTGTGATCAGAAATTGG+AGG + chr8.3:31486404-31486423 MS.gene82986:CDS 30.0%
ACAATTACACTAGTAGCAAT+CGG - chr8.3:31486291-31486310 None:intergenic 30.0%
AGAAATTGTTAAAAGGTTCC+AGG - chr8.3:31485423-31485442 None:intergenic 30.0%
ATATATTTGGACTCGAACTA+TGG - chr8.3:31487340-31487359 None:intergenic 30.0%
ATTGCATATAACATGACATG+AGG - chr8.3:31487404-31487423 None:intergenic 30.0%
ATTTGTGATCAGAAATTGGA+GGG + chr8.3:31486405-31486424 MS.gene82986:CDS 30.0%
CATCTATTTGGAAATGTACT+AGG - chr8.3:31487194-31487213 None:intergenic 30.0%
CCAAATTAAGGTTGAGATTT+AGG - chr8.3:31485627-31485646 None:intergenic 30.0%
CCTAAATCTCAACCTTAATT+TGG + chr8.3:31485624-31485643 MS.gene82986:CDS 30.0%
CTAGTACATTTCCAAATAGA+TGG + chr8.3:31487192-31487211 MS.gene82986:CDS 30.0%
GAGAAATAGAAGTAGAAGAA+TGG - chr8.3:31485243-31485262 None:intergenic 30.0%
GCAAATTTGTGATCAGAAAT+TGG + chr8.3:31486401-31486420 MS.gene82986:CDS 30.0%
GCTAGGGATATTAAGAAAAT+TGG + chr8.3:31486660-31486679 MS.gene82986:CDS 30.0%
GGAAGATACTCATAATAAAG+AGG - chr8.3:31486786-31486805 None:intergenic 30.0%
GGATATTAAGAAAATTGGGT+TGG + chr8.3:31486665-31486684 MS.gene82986:CDS 30.0%
GGTGATATTATAATCTACAC+TGG + chr8.3:31486348-31486367 MS.gene82986:CDS 30.0%
GTTAGTTGCTTGTTTGATAT+AGG + chr8.3:31486555-31486574 MS.gene82986:CDS 30.0%
TCAATAGAGATACAACTCAT+TGG - chr8.3:31486699-31486718 None:intergenic 30.0%
TCATGTCATGTTATATGCAA+TGG + chr8.3:31487403-31487422 MS.gene82986:CDS 30.0%
TCCAGAAAATAATCTAAAGG+AGG - chr8.3:31487281-31487300 None:intergenic 30.0%
TGATGAGAAATACTAAGATC+GGG - chr8.3:31485301-31485320 None:intergenic 30.0%
TTGCATATAACATGACATGA+GGG - chr8.3:31487403-31487422 None:intergenic 30.0%
TTGTTTGATATAGGAAAGTC+TGG + chr8.3:31486564-31486583 MS.gene82986:CDS 30.0%
! AAATCTCAACCTTAATTTGG+GGG + chr8.3:31485627-31485646 MS.gene82986:CDS 30.0%
! AATTGGTTGTTGAAGTTGAT+AGG + chr8.3:31485890-31485909 MS.gene82986:CDS 30.0%
! ACCTCCTTTAGATTATTTTC+TGG + chr8.3:31487277-31487296 MS.gene82986:CDS 30.0%
! AGAGAAATTAGGTTGTTGTT+GGG - chr8.3:31485461-31485480 None:intergenic 30.0%
! TTTGATGGCATATATGGTTA+TGG + chr8.3:31485714-31485733 MS.gene82986:CDS 30.0%
!! AATATAGCTTGGAAGTGTTT+AGG + chr8.3:31487033-31487052 MS.gene82986:CDS 30.0%
!! ATTGGTTGTTGAAGTTGATA+GGG + chr8.3:31485891-31485910 MS.gene82986:CDS 30.0%
!! TAATCTAAAGGAGGTATTGA+CGG - chr8.3:31487272-31487291 None:intergenic 30.0%
!! TGATGTGTTTTTGTTTAGGA+GGG + chr8.3:31486185-31486204 MS.gene82986:CDS 30.0%
!! TGTTGTTTCATTGGAGAATA+CGG + chr8.3:31485582-31485601 MS.gene82986:CDS 30.0%
!!! TTGGTGATGTGTTTTTGTTT+AGG + chr8.3:31486181-31486200 MS.gene82986:CDS 30.0%
AAATGATCGAGCTATTGTTG+TGG + chr8.3:31485669-31485688 MS.gene82986:CDS 35.0%
AATCAGCGATTTCGAGAAAA+GGG - chr8.3:31485549-31485568 None:intergenic 35.0%
ACATCACCAACATAAATTCC+AGG - chr8.3:31486171-31486190 None:intergenic 35.0%
ACCACATTCATGGTTATATG+AGG + chr8.3:31486079-31486098 MS.gene82986:CDS 35.0%
ACTAGTGTAATTGTTTCAGG+TGG + chr8.3:31486297-31486316 MS.gene82986:CDS 35.0%
AGAGAACTCTGCATAGATTA+TGG + chr8.3:31487443-31487462 MS.gene82986:CDS 35.0%
ATAGGGAAAATGTTCCAGTT+AGG + chr8.3:31486730-31486749 MS.gene82986:CDS 35.0%
ATGAGAAATACTAAGATCGG+GGG - chr8.3:31485299-31485318 None:intergenic 35.0%
ATTATAATCTACACTGGCCA+TGG + chr8.3:31486354-31486373 MS.gene82986:CDS 35.0%
ATTGGATGAGGAGAAGATAT+TGG + chr8.3:31485945-31485964 MS.gene82986:CDS 35.0%
ATTGTCTGAGGAAAGGAAAT+TGG + chr8.3:31485927-31485946 MS.gene82986:CDS 35.0%
CCAACATAAATTCCAGGTAT+CGG - chr8.3:31486165-31486184 None:intergenic 35.0%
CCACATTCATGGTTATATGA+GGG + chr8.3:31486080-31486099 MS.gene82986:CDS 35.0%
GACGCGAAGAAATTGTTAAA+AGG - chr8.3:31485430-31485449 None:intergenic 35.0%
GATGAGAAATACTAAGATCG+GGG - chr8.3:31485300-31485319 None:intergenic 35.0%
GCTACTAGTGTAATTGTTTC+AGG + chr8.3:31486294-31486313 MS.gene82986:CDS 35.0%
GGGAGACAATTAATGGAATA+AGG + chr8.3:31486043-31486062 MS.gene82986:CDS 35.0%
GGTAAGGTTTATGTTTACGA+TGG + chr8.3:31486519-31486538 MS.gene82986:CDS 35.0%
TAGAATTTGGCGTATAGGTT+GGG - chr8.3:31485508-31485527 None:intergenic 35.0%
TATCGTGATCAAAGGATAGT+TGG + chr8.3:31486138-31486157 MS.gene82986:CDS 35.0%
TATGGTTGTATCGTGATCAA+AGG + chr8.3:31486130-31486149 MS.gene82986:CDS 35.0%
TCCAAATAGATGGGTAGAAT+GGG + chr8.3:31487202-31487221 MS.gene82986:CDS 35.0%
TGCACTTTCGTCATAAGATA+AGG + chr8.3:31485797-31485816 MS.gene82986:CDS 35.0%
TTAAGGTTGAGATTTAGGTC+CGG - chr8.3:31485622-31485641 None:intergenic 35.0%
TTCCAAATAGATGGGTAGAA+TGG + chr8.3:31487201-31487220 MS.gene82986:CDS 35.0%
TTCGTCATAAGATAAGGAAG+AGG + chr8.3:31485803-31485822 MS.gene82986:CDS 35.0%
TTGTTAAAAGGTTCCAGGTT+TGG - chr8.3:31485418-31485437 None:intergenic 35.0%
! AAGCAATCCATGTGGATTAT+AGG - chr8.3:31486925-31486944 None:intergenic 35.0%
! AAGTAGAAGAATGGGTTTCA+AGG - chr8.3:31485234-31485253 None:intergenic 35.0%
! AATGGGTTTCAAGGGTTAAT+GGG - chr8.3:31485225-31485244 None:intergenic 35.0%
! AATTGTTTCAGGTGGTTATG+AGG + chr8.3:31486305-31486324 MS.gene82986:CDS 35.0%
! AGCAATCCATGTGGATTATA+GGG - chr8.3:31486924-31486943 None:intergenic 35.0%
! AGTAGAAGAATGGGTTTCAA+GGG - chr8.3:31485233-31485252 None:intergenic 35.0%
! GAGAGAAATTAGGTTGTTGT+TGG - chr8.3:31485462-31485481 None:intergenic 35.0%
!! ATACTGGTGTTGCTATAACT+AGG + chr8.3:31487123-31487142 MS.gene82986:CDS 35.0%
!! CACATGGATTGCTTTTGAAA+GGG + chr8.3:31486931-31486950 MS.gene82986:CDS 35.0%
!! GATATAGGAAAGTCTGGTTT+TGG + chr8.3:31486570-31486589 MS.gene82986:CDS 35.0%
!! GTGATGTGTTTTTGTTTAGG+AGG + chr8.3:31486184-31486203 MS.gene82986:CDS 35.0%
!! TACTGGTGTTGCTATAACTA+GGG + chr8.3:31487124-31487143 MS.gene82986:CDS 35.0%
!! TTGATCATCGGTTTCAGATT+CGG - chr8.3:31485485-31485504 None:intergenic 35.0%
!!! TTTTGAGGTGTGGTAGAATT+TGG - chr8.3:31485521-31485540 None:intergenic 35.0%
AAAGGGGCTCAAATATAGCT+TGG + chr8.3:31487022-31487041 MS.gene82986:CDS 40.0%
AAGTGATGTTCCGATGGATA+TGG - chr8.3:31485380-31485399 None:intergenic 40.0%
ACCAGTATACTCGCAGATAA+AGG - chr8.3:31487111-31487130 None:intergenic 40.0%
ACCTAGTGGTTGTGAATGTA+AGG + chr8.3:31486848-31486867 MS.gene82986:CDS 40.0%
ACTCGAACTATGGCTCATAT+AGG - chr8.3:31487330-31487349 None:intergenic 40.0%
AGAAGAGATTGTCTGAGGAA+AGG + chr8.3:31485920-31485939 MS.gene82986:CDS 40.0%
ATTGAGGTTAGGGTTATTCG+CGG + chr8.3:31486465-31486484 MS.gene82986:CDS 40.0%
CAAATCACCACCATATCCAT+CGG + chr8.3:31485367-31485386 MS.gene82986:CDS 40.0%
CACTGTTCTCAAAGATGCTA+GGG + chr8.3:31486644-31486663 MS.gene82986:CDS 40.0%
CCAAATAGATGGGTAGAATG+GGG + chr8.3:31487203-31487222 MS.gene82986:CDS 40.0%
CCCTCATATAACCATGAATG+TGG - chr8.3:31486083-31486102 None:intergenic 40.0%
CCGATACCTGGAATTTATGT+TGG + chr8.3:31486162-31486181 MS.gene82986:CDS 40.0%
CCTTTCAAAAGCAATCCATG+TGG - chr8.3:31486933-31486952 None:intergenic 40.0%
CGTAGTATCAACCACATTCA+TGG + chr8.3:31486069-31486088 MS.gene82986:CDS 40.0%
CTCTGATGAAACACAAACCA+CGG - chr8.3:31486822-31486841 None:intergenic 40.0%
CTGATCACAAATTTGCCTTG+AGG - chr8.3:31486397-31486416 None:intergenic 40.0%
GAAACAAGAGTCGAGTTTGA+TGG + chr8.3:31485699-31485718 MS.gene82986:CDS 40.0%
GAATCAGCGATTTCGAGAAA+AGG - chr8.3:31485550-31485569 None:intergenic 40.0%
GAGTTTCCCTATAATCCACA+TGG + chr8.3:31486915-31486934 MS.gene82986:CDS 40.0%
GATGACATGGAATTAGACGA+TGG + chr8.3:31486327-31486346 MS.gene82986:CDS 40.0%
GGAATAGGAACATGAGAAGA+AGG - chr8.3:31485268-31485287 None:intergenic 40.0%
GGCATATATGGTTATGGAGA+AGG + chr8.3:31485720-31485739 MS.gene82986:CDS 40.0%
GGTTTCAGATCTGAGTATGA+AGG + chr8.3:31485768-31485787 MS.gene82986:CDS 40.0%
GGTTTGTGTTTCATCAGAGT+GGG + chr8.3:31486823-31486842 MS.gene82986:CDS 40.0%
GTAGAATTTGGCGTATAGGT+TGG - chr8.3:31485509-31485528 None:intergenic 40.0%
GTTTCAAGGGTTAATGGGAA+AGG - chr8.3:31485220-31485239 None:intergenic 40.0%
GTTTGTGTTTCATCAGAGTG+GGG + chr8.3:31486824-31486843 MS.gene82986:CDS 40.0%
TAAAGGATCCAGCCTGAATA+AGG - chr8.3:31487094-31487113 None:intergenic 40.0%
TAAGGTTGAGATTTAGGTCC+GGG - chr8.3:31485621-31485640 None:intergenic 40.0%
TACGGTAAATGTTTGCAACC+CGG + chr8.3:31485600-31485619 MS.gene82986:CDS 40.0%
TAGGAGGACATGAGCAAAAA+GGG - chr8.3:31486980-31486999 None:intergenic 40.0%
TAGTGGTTGTGAATGTAAGG+AGG + chr8.3:31486851-31486870 MS.gene82986:CDS 40.0%
TCAGATTCGGGAGAGAAATT+AGG - chr8.3:31485472-31485491 None:intergenic 40.0%
TCCTTACATTCACAACCACT+AGG - chr8.3:31486852-31486871 None:intergenic 40.0%
TCCTTTATCTGCGAGTATAC+TGG + chr8.3:31487107-31487126 MS.gene82986:CDS 40.0%
TCTATGCAGAGTTCTCTCAT+AGG - chr8.3:31487440-31487459 None:intergenic 40.0%
TCTGATGAAACACAAACCAC+GGG - chr8.3:31486821-31486840 None:intergenic 40.0%
TGAGGAAAGGAAATTGGATG+AGG + chr8.3:31485933-31485952 MS.gene82986:CDS 40.0%
TGTGGTAGAATTTGGCGTAT+AGG - chr8.3:31485513-31485532 None:intergenic 40.0%
TTAGGAGGACATGAGCAAAA+AGG - chr8.3:31486981-31487000 None:intergenic 40.0%
TTGCATATGCATATGCAAGC+TGG + chr8.3:31486225-31486244 MS.gene82986:CDS 40.0%
! AAACTCGACTCTTGTTTCTC+AGG - chr8.3:31485697-31485716 None:intergenic 40.0%
! AGGTTGTTGTTGGGAATCTT+GGG - chr8.3:31485452-31485471 None:intergenic 40.0%
! ATGGATATGGTGGTGATTTG+GGG - chr8.3:31485367-31485386 None:intergenic 40.0%
! GAATGGGTTTCAAGGGTTAA+TGG - chr8.3:31485226-31485245 None:intergenic 40.0%
! GAGAAAAGGGTAGAGTTTTG+AGG - chr8.3:31485536-31485555 None:intergenic 40.0%
! GATGGATATGGTGGTGATTT+GGG - chr8.3:31485368-31485387 None:intergenic 40.0%
! GTCGAGTTTGATGGCATATA+TGG + chr8.3:31485708-31485727 MS.gene82986:CDS 40.0%
! TAGGTTGTTGTTGGGAATCT+TGG - chr8.3:31485453-31485472 None:intergenic 40.0%
! TGCTTTTGCGCTATGAAGAA+TGG + chr8.3:31486888-31486907 MS.gene82986:CDS 40.0%
! TGGAAAGGAGTATGCACTAT+GGG + chr8.3:31486442-31486461 MS.gene82986:CDS 40.0%
! TGGTTTGTGTTTCATCAGAG+TGG + chr8.3:31486822-31486841 MS.gene82986:CDS 40.0%
! TTGGAAAGGAGTATGCACTA+TGG + chr8.3:31486441-31486460 MS.gene82986:CDS 40.0%
! TTTTGCGCTATGAAGAATGG+AGG + chr8.3:31486891-31486910 MS.gene82986:CDS 40.0%
!! CCACATGGATTGCTTTTGAA+AGG + chr8.3:31486930-31486949 MS.gene82986:CDS 40.0%
!! GTATAGGTTGGGTTGATCAT+CGG - chr8.3:31485497-31485516 None:intergenic 40.0%
!! TGATCATCGGTTTCAGATTC+GGG - chr8.3:31485484-31485503 None:intergenic 40.0%
AACAATGAATGGGGTCTCGT+TGG + chr8.3:31487163-31487182 MS.gene82986:CDS 45.0%
AACCACGGGAAAGTAGTATC+CGG - chr8.3:31486807-31486826 None:intergenic 45.0%
ACACGGTTTCGACAATTAGG+AGG - chr8.3:31486996-31487015 None:intergenic 45.0%
AGGTGGTTATGAGGATGACA+TGG + chr8.3:31486314-31486333 MS.gene82986:CDS 45.0%
ATAATCTACACTGGCCATGG+AGG + chr8.3:31486357-31486376 MS.gene82986:CDS 45.0%
ATGCAGAGTTCTCTCATAGG+AGG - chr8.3:31487437-31487456 None:intergenic 45.0%
ATTCGCGGGATGAAGTATGA+AGG + chr8.3:31486480-31486499 MS.gene82986:CDS 45.0%
CAGAATCCACCCCCAAATTA+AGG - chr8.3:31485639-31485658 None:intergenic 45.0%
CCCCATTCTACCCATCTATT+TGG - chr8.3:31487206-31487225 None:intergenic 45.0%
CGAAACCGTGTTGCACAAAA+GGG + chr8.3:31487005-31487024 MS.gene82986:CDS 45.0%
CTGCATAGATTATGGCATCC+CGG + chr8.3:31487451-31487470 MS.gene82986:CDS 45.0%
GAAACCGTGTTGCACAAAAG+GGG + chr8.3:31487006-31487025 MS.gene82986:CDS 45.0%
GAGTATGCACTATGGGATTG+AGG + chr8.3:31486449-31486468 MS.gene82986:CDS 45.0%
GAGTGCATCTACTTCTGGTA+AGG + chr8.3:31486503-31486522 MS.gene82986:CDS 45.0%
GCAACACGGTTTCGACAATT+AGG - chr8.3:31486999-31487018 None:intergenic 45.0%
GCACTATGGGATTGAGGTTA+GGG + chr8.3:31486455-31486474 MS.gene82986:CDS 45.0%
GCACTGTTCTCAAAGATGCT+AGG + chr8.3:31486643-31486662 MS.gene82986:CDS 45.0%
GCATACTCCTTTCCAATGCA+AGG - chr8.3:31486437-31486456 None:intergenic 45.0%
GGAAGAGAAGAGATTGTCTG+AGG + chr8.3:31485915-31485934 MS.gene82986:CDS 45.0%
GGAGGACAAGAGAATGAATG+AGG + chr8.3:31485861-31485880 MS.gene82986:CDS 45.0%
GGGCCGCATTCAAAAATCAA+AGG - chr8.3:31486960-31486979 None:intergenic 45.0%
GTGGAAACCTTGCATTGGAA+AGG + chr8.3:31486427-31486446 MS.gene82986:CDS 45.0%
GTGTTTAGGTCTACACAGAC+AGG + chr8.3:31487047-31487066 MS.gene82986:CDS 45.0%
GTTATTGAGAATTGAGGGGC+AGG + chr8.3:31486608-31486627 MS.gene82986:CDS 45.0%
TAATCTACACTGGCCATGGA+GGG + chr8.3:31486358-31486377 MS.gene82986:CDS 45.0%
TAGGTCCTTGGACCTTATTC+AGG + chr8.3:31487079-31487098 MS.gene82986:CDS 45.0%
TAGGTCTACACAGACAGGTT+GGG + chr8.3:31487052-31487071 MS.gene82986:CDS 45.0%
TCGAAACCGTGTTGCACAAA+AGG + chr8.3:31487004-31487023 MS.gene82986:CDS 45.0%
TGATGTTCCGATGGATATGG+TGG - chr8.3:31485377-31485396 None:intergenic 45.0%
TGCACTATGGGATTGAGGTT+AGG + chr8.3:31486454-31486473 MS.gene82986:CDS 45.0%
TGCAGAGTTCTCTCATAGGA+GGG - chr8.3:31487436-31487455 None:intergenic 45.0%
TGTAAGGAGGCTTGTACTGA+CGG + chr8.3:31486864-31486883 MS.gene82986:CDS 45.0%
TGTGATCAGAAATTGGAGGG+TGG + chr8.3:31486408-31486427 MS.gene82986:CDS 45.0%
TTAGGTCTACACAGACAGGT+TGG + chr8.3:31487051-31487070 MS.gene82986:CDS 45.0%
TTCGCGGGATGAAGTATGAA+GGG + chr8.3:31486481-31486500 MS.gene82986:CDS 45.0%
TTGAGGTTAGGGTTATTCGC+GGG + chr8.3:31486466-31486485 MS.gene82986:CDS 45.0%
! ATATGGTGGTGATTTGGGGT+GGG - chr8.3:31485363-31485382 None:intergenic 45.0%
! CGACATTAATGAAGGAGCGT+GGG + chr8.3:31486106-31486125 MS.gene82986:CDS 45.0%
! CGATGGATATGGTGGTGATT+TGG - chr8.3:31485369-31485388 None:intergenic 45.0%
! CGTTTGAAGTGATGTTCCGA+TGG - chr8.3:31485386-31485405 None:intergenic 45.0%
! GGGGTGGATTCTGTAAATGA+TGG + chr8.3:31485646-31485665 MS.gene82986:CDS 45.0%
! TCTCAACCTTAATTTGGGGG+TGG + chr8.3:31485630-31485649 MS.gene82986:CDS 45.0%
!! GGAGGTATTGACGGATAAGA+TGG - chr8.3:31487263-31487282 None:intergenic 45.0%
!! GGTTGTTGAAGTTGATAGGG+AGG + chr8.3:31485894-31485913 MS.gene82986:CDS 45.0%
!!! AAGGGTAGAGTTTTGAGGTG+TGG - chr8.3:31485531-31485550 None:intergenic 45.0%
AACGCCACTCATTCCAAACC+TGG + chr8.3:31485402-31485421 MS.gene82986:CDS 50.0%
AATTCTTCTCTCGCCCTCCA+TGG - chr8.3:31486374-31486393 None:intergenic 50.0%
AGGAGGGAATTGTGTGTGAC+TGG + chr8.3:31486201-31486220 MS.gene82986:CDS 50.0%
AGGATAGTTGGACCGATACC+TGG + chr8.3:31486150-31486169 MS.gene82986:CDS 50.0%
AGGTCTACACAGACAGGTTG+GGG + chr8.3:31487053-31487072 MS.gene82986:CDS 50.0%
CTAAGATCGGGGGTGATAGT+AGG - chr8.3:31485289-31485308 None:intergenic 50.0%
GAGAGTGGGTGAGGAGAATA+GGG + chr8.3:31485999-31486018 MS.gene82986:CDS 50.0%
GAGGGCTGCGACATTAATGA+AGG + chr8.3:31486098-31486117 MS.gene82986:CDS 50.0%
GCGACATTAATGAAGGAGCG+TGG + chr8.3:31486105-31486124 MS.gene82986:CDS 50.0%
GGATGCAGAAGTTGCAGTTG+TGG + chr8.3:31485966-31485985 MS.gene82986:CDS 50.0%
GGCGAGAGAAGAATTCCTCA+AGG + chr8.3:31486379-31486398 MS.gene82986:CDS 50.0%
GGTGAGAGCGGTGTGATTTA+AGG - chr8.3:31485341-31485360 None:intergenic 50.0%
GGTTATGGAGAAGGAAAGCC+AGG + chr8.3:31485729-31485748 MS.gene82986:CDS 50.0%
GTGGAGGGGGAGACAATTAA+TGG + chr8.3:31486036-31486055 MS.gene82986:CDS 50.0%
TCCAGCCTGAATAAGGTCCA+AGG - chr8.3:31487087-31487106 None:intergenic 50.0%
TCCTTGGACCTTATTCAGGC+TGG + chr8.3:31487083-31487102 MS.gene82986:CDS 50.0%
TCGGGGGTGATAGTAGGAAT+AGG - chr8.3:31485283-31485302 None:intergenic 50.0%
TGAGAGTGGGTGAGGAGAAT+AGG + chr8.3:31485998-31486017 MS.gene82986:CDS 50.0%
TGCAGAAGTTGCAGTTGTGG+AGG + chr8.3:31485969-31485988 MS.gene82986:CDS 50.0%
TTCCGGATACTACTTTCCCG+TGG + chr8.3:31486802-31486821 MS.gene82986:CDS 50.0%
TTCGCTTCGCGTGAGAATTG+AGG + chr8.3:31485840-31485859 MS.gene82986:CDS 50.0%
! GAAGGGAGTGCATCTACTTC+TGG + chr8.3:31486498-31486517 MS.gene82986:CDS 50.0%
! GATATGGTGGTGATTTGGGG+TGG - chr8.3:31485364-31485383 None:intergenic 50.0%
! GGTTCCAGGTTTGGAATGAG+TGG - chr8.3:31485409-31485428 None:intergenic 50.0%
! TAATGAAGGAGCGTGGGCTA+TGG + chr8.3:31486112-31486131 MS.gene82986:CDS 50.0%
! TATGGTGGTGATTTGGGGTG+GGG - chr8.3:31485362-31485381 None:intergenic 50.0%
! TGAGCCCCTTTTGTGCAACA+CGG - chr8.3:31487013-31487032 None:intergenic 50.0%
!! CCAGGTGATGGAACTTGTGA+GGG + chr8.3:31485747-31485766 MS.gene82986:CDS 50.0%
AATTGAGGGGCAGGCGAAGA+TGG + chr8.3:31486617-31486636 MS.gene82986:CDS 55.0%
AATTGTCTCCCCCTCCACGT+TGG - chr8.3:31486034-31486053 None:intergenic 55.0%
AGGTTGGGGAGTTAGGTCCT+TGG + chr8.3:31487067-31487086 MS.gene82986:CDS 55.0%
ATTATGGCATCCCGGACGAG+TGG + chr8.3:31487459-31487478 MS.gene82986:CDS 55.0%
ATTGAGGGGCAGGCGAAGAT+GGG + chr8.3:31486618-31486637 MS.gene82986:CDS 55.0%
CATCAGAGTGGGGAACCTAG+TGG + chr8.3:31486834-31486853 MS.gene82986:CDS 55.0%
CCCTCACAAGTTCCATCACC+TGG - chr8.3:31485750-31485769 None:intergenic 55.0%
GCTTCGCGTGAGAATTGAGG+AGG + chr8.3:31485843-31485862 MS.gene82986:CDS 55.0%
GGAGAAGGAAAGCCAGGTGA+TGG + chr8.3:31485735-31485754 MS.gene82986:CDS 55.0%
GGAGGGTGGAAACCTTGCAT+TGG + chr8.3:31486422-31486441 MS.gene82986:CDS 55.0%
GGGTGAGGAGAATAGGGTGT+CGG + chr8.3:31486005-31486024 MS.gene82986:CDS 55.0%
GTGGAGGCAGAGATGAGAGT+GGG + chr8.3:31485985-31486004 MS.gene82986:CDS 55.0%
TGTGGAGGCAGAGATGAGAG+TGG + chr8.3:31485984-31486003 MS.gene82986:CDS 55.0%
TTATGGCATCCCGGACGAGT+GGG + chr8.3:31487460-31487479 MS.gene82986:CDS 55.0%
! CACAGACAGGTTGGGGAGTT+AGG + chr8.3:31487060-31487079 MS.gene82986:CDS 55.0%
!! GCCAGGTGATGGAACTTGTG+AGG + chr8.3:31485746-31485765 MS.gene82986:CDS 55.0%
ATCTTGCTCGCCCACTCGTC+CGG - chr8.3:31487473-31487492 None:intergenic 60.0%
GGCAGAGATGAGAGTGGGTG+AGG + chr8.3:31485990-31486009 MS.gene82986:CDS 60.0%
! GATTTGGGGTGGGGTGAGAG+CGG - chr8.3:31485353-31485372 None:intergenic 60.0%
! TGTCGGCTACCAACGTGGAG+GGG + chr8.3:31486022-31486041 MS.gene82986:CDS 60.0%
!! GTGTCGGCTACCAACGTGGA+GGG + chr8.3:31486021-31486040 MS.gene82986:CDS 60.0%
!! TAGGGTGTCGGCTACCAACG+TGG + chr8.3:31486017-31486036 MS.gene82986:CDS 60.0%
GTCGGCTACCAACGTGGAGG+GGG + chr8.3:31486023-31486042 MS.gene82986:CDS 65.0%
TCTTGCTCGCCCACTCGTCC+GGG - chr8.3:31487472-31487491 None:intergenic 65.0%
!! GGTGTCGGCTACCAACGTGG+AGG + chr8.3:31486020-31486039 MS.gene82986:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 31485211 31487508 31485211 ID=MS.gene82986
chr8.3 mRNA 31485211 31487508 31485211 ID=MS.gene82986.t1;Parent=MS.gene82986
chr8.3 exon 31485211 31487508 31485211 ID=MS.gene82986.t1.exon1;Parent=MS.gene82986.t1
chr8.3 CDS 31485211 31487508 31485211 ID=cds.MS.gene82986.t1;Parent=MS.gene82986.t1
Gene Sequence

>MS.gene82986

ATGGATCCTTTCCCATTAACCCTTGAAACCCATTCTTCTACTTCTATTTCTCAACCTTCTTCTCATGTTCCTATTCCTACTATCACCCCCGATCTTAGTATTTCTCATCAAATCCCTAGAATTTCAACCTTAAATCACACCGCTCTCACCCCACCCCAAATCACCACCATATCCATCGGAACATCACTTCAAACGCCACTCATTCCAAACCTGGAACCTTTTAACAATTTCTTCGCGTCCCAAGATTCCCAACAACAACCTAATTTCTCTCCCGAATCTGAAACCGATGATCAACCCAACCTATACGCCAAATTCTACCACACCTCAAAACTCTACCCTTTTCTCGAAATCGCTGATTCCAACAATGATTTTGTTGTTTCATTGGAGAATACGGTAAATGTTTGCAACCCGGACCTAAATCTCAACCTTAATTTGGGGGTGGATTCTGTAAATGATGGAAATGATCGAGCTATTGTTGTGGCTCCTGAGAAACAAGAGTCGAGTTTGATGGCATATATGGTTATGGAGAAGGAAAGCCAGGTGATGGAACTTGTGAGGGTTTCAGATCTGAGTATGAAGGATCAGATGCACTTTCGTCATAAGATAAGGAAGAGGCGAATGATTTATGATTCGCTTCGCGTGAGAATTGAGGAGGACAAGAGAATGAATGAGGAGAAGAAATTGGTTGTTGAAGTTGATAGGGAGGAAGAGAAGAGATTGTCTGAGGAAAGGAAATTGGATGAGGAGAAGATATTGGATGCAGAAGTTGCAGTTGTGGAGGCAGAGATGAGAGTGGGTGAGGAGAATAGGGTGTCGGCTACCAACGTGGAGGGGGAGACAATTAATGGAATAAGGCGTCGTAGTATCAACCACATTCATGGTTATATGAGGGCTGCGACATTAATGAAGGAGCGTGGGCTATGGTTGTATCGTGATCAAAGGATAGTTGGACCGATACCTGGAATTTATGTTGGTGATGTGTTTTTGTTTAGGAGGGAATTGTGTGTGACTGGTTTGCATATGCATATGCAAGCTGGTATTGATTATCTATCAAAGAGTATGTCTTATAATGGTGAACCGATTGCTACTAGTGTAATTGTTTCAGGTGGTTATGAGGATGACATGGAATTAGACGATGGTGATATTATAATCTACACTGGCCATGGAGGGCGAGAGAAGAATTCCTCAAGGCAAATTTGTGATCAGAAATTGGAGGGTGGAAACCTTGCATTGGAAAGGAGTATGCACTATGGGATTGAGGTTAGGGTTATTCGCGGGATGAAGTATGAAGGGAGTGCATCTACTTCTGGTAAGGTTTATGTTTACGATGGATTGTATAAGATTGTTAGTTGCTTGTTTGATATAGGAAAGTCTGGTTTTGGAGTTTATAAGTATAAGTTATTGAGAATTGAGGGGCAGGCGAAGATGGGTAGCACTGTTCTCAAAGATGCTAGGGATATTAAGAAAATTGGGTTGGATTTCATGCCAATGAGTTGTATCTCTATTGATATTTCAAATAATAGGGAAAATGTTCCAGTTAGGCTATTTAATGATATAGATGTCAATCATGAACCTCTTTATTATGAGTATCTTCCGGATACTACTTTCCCGTGGTTTGTGTTTCATCAGAGTGGGGAACCTAGTGGTTGTGAATGTAAGGAGGCTTGTACTGACGGTTGCTTTTGCGCTATGAAGAATGGAGGAGAGTTTCCCTATAATCCACATGGATTGCTTTTGAAAGGGAAACCTTTGATTTTTGAATGCGGCCCTTTTTGCTCATGTCCTCCTAATTGTCGAAACCGTGTTGCACAAAAGGGGCTCAAATATAGCTTGGAAGTGTTTAGGTCTACACAGACAGGTTGGGGAGTTAGGTCCTTGGACCTTATTCAGGCTGGATCCTTTATCTGCGAGTATACTGGTGTTGCTATAACTAGGGAACAAGCTCAAATTTTAACAATGAATGGGGTCTCGTTGGTGTATCCTAGTACATTTCCAAATAGATGGGTAGAATGGGGTGATTTATCTCAGATATACACTGAATATGTGCGACCATCTTATCCGTCAATACCTCCTTTAGATTATTTTCTGGATGTGTCAACAATGAGAAATGTTGCATCCTATATGAGCCATAGTTCGAGTCCAAATATATTCATTCAGTATGTTCTTTATGATCACAACAATTTGTTGTTCCCTCATGTCATGTTATATGCAATGGAGAACATCCCTCCTATGAGAGAACTCTGCATAGATTATGGCATCCCGGACGAGTGGGCGAGCAAGATGTCTATATGTATGTAA
Protein sequence

>MS.gene82986.t1

MDPFPLTLETHSSTSISQPSSHVPIPTITPDLSISHQIPRISTLNHTALTPPQITTISIGTSLQTPLIPNLEPFNNFFASQDSQQQPNFSPESETDDQPNLYAKFYHTSKLYPFLEIADSNNDFVVSLENTVNVCNPDLNLNLNLGVDSVNDGNDRAIVVAPEKQESSLMAYMVMEKESQVMELVRVSDLSMKDQMHFRHKIRKRRMIYDSLRVRIEEDKRMNEEKKLVVEVDREEEKRLSEERKLDEEKILDAEVAVVEAEMRVGEENRVSATNVEGETINGIRRRSINHIHGYMRAATLMKERGLWLYRDQRIVGPIPGIYVGDVFLFRRELCVTGLHMHMQAGIDYLSKSMSYNGEPIATSVIVSGGYEDDMELDDGDIIIYTGHGGREKNSSRQICDQKLEGGNLALERSMHYGIEVRVIRGMKYEGSASTSGKVYVYDGLYKIVSCLFDIGKSGFGVYKYKLLRIEGQAKMGSTVLKDARDIKKIGLDFMPMSCISIDISNNRENVPVRLFNDIDVNHEPLYYEYLPDTTFPWFVFHQSGEPSGCECKEACTDGCFCAMKNGGEFPYNPHGLLLKGKPLIFECGPFCSCPPNCRNRVAQKGLKYSLEVFRSTQTGWGVRSLDLIQAGSFICEYTGVAITREQAQILTMNGVSLVYPSTFPNRWVEWGDLSQIYTEYVRPSYPSIPPLDYFLDVSTMRNVASYMSHSSSPNIFIQYVLYDHNNLLFPHVMLYAMENIPPMRELCIDYGIPDEWASKMSICM