AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82987

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82987.t1 MTR_8g071030 86.417 773 73 3 1 765 1 749 0.0 1310
MS.gene82987.t1 MTR_8g070070 74.520 781 171 8 1 765 1 769 0.0 1145
MS.gene82987.t1 MTR_8g070075 64.713 785 223 9 1 761 4 758 0.0 1009
MS.gene82987.t1 MTR_1g035420 63.197 538 183 6 229 764 134 658 0.0 697
MS.gene82987.t1 MTR_1g035400 61.702 470 177 3 288 756 101 568 0.0 608
MS.gene82987.t1 MTR_6g044690 57.419 465 175 8 298 753 114 564 0.0 538
MS.gene82987.t1 MTR_0376s0030 51.176 510 222 8 267 764 83 577 4.55e-172 507
MS.gene82987.t1 MTR_6g043960 51.509 464 183 8 298 753 53 482 1.60e-157 466
MS.gene82987.t1 MTR_2g054460 44.112 569 201 13 194 753 4 464 4.51e-144 431
MS.gene82987.t1 MTR_7g088370 43.312 471 225 17 296 753 239 680 6.41e-107 342
MS.gene82987.t1 MTR_5g013420 40.000 460 253 8 296 751 618 1058 1.98e-105 348
MS.gene82987.t1 MTR_5g013420 40.000 460 253 8 296 751 618 1058 2.17e-105 348
MS.gene82987.t1 MTR_3g011440 36.315 559 321 13 204 752 100 633 1.47e-104 335
MS.gene82987.t1 MTR_4g010830 40.741 459 250 9 296 751 510 949 3.80e-102 337
MS.gene82987.t1 MTR_8g042750 40.381 473 249 13 296 753 204 658 9.57e-102 328
MS.gene82987.t1 MTR_4g010550 38.961 462 224 12 296 751 24 433 1.49e-93 300
MS.gene82987.t1 MTR_7g084090 37.967 482 259 14 296 752 261 727 1.20e-87 293
MS.gene82987.t1 MTR_6g061270 37.838 481 260 13 296 752 199 664 2.23e-86 288
MS.gene82987.t1 MTR_6g061200 37.421 473 244 15 296 752 199 635 1.27e-80 271
MS.gene82987.t1 MTR_7g084090 41.322 363 183 11 296 641 261 610 3.50e-72 249
MS.gene82987.t1 MTR_7g445710 31.875 480 228 17 295 751 109 512 5.23e-54 196
MS.gene82987.t1 MTR_7g445530 31.667 480 229 17 295 751 109 512 7.28e-54 194
MS.gene82987.t1 MTR_1g048950 32.979 470 264 15 298 751 73 507 4.36e-52 191
MS.gene82987.t1 MTR_7g450730 30.349 458 227 15 296 751 107 474 7.33e-51 186
MS.gene82987.t1 MTR_7g052480 31.613 310 186 9 459 752 227 526 1.13e-38 152
MS.gene82987.t1 MTR_7g445600 34.667 150 61 2 602 751 243 355 1.33e-22 100
MS.gene82987.t1 MTR_7g445600 37.984 129 66 5 296 424 106 220 4.64e-15 77.8
MS.gene82987.t1 MTR_6g044000 67.925 53 17 0 701 753 117 169 1.21e-20 90.1
MS.gene82987.t1 MTR_6g044000 52.941 68 32 0 298 365 53 120 2.47e-18 83.6
MS.gene82987.t1 MTR_8g078490 29.730 185 118 3 568 751 1056 1229 3.72e-20 96.7
MS.gene82987.t1 MTR_5g018850 31.492 181 112 3 568 747 1310 1479 4.26e-20 96.7
MS.gene82987.t1 MTR_8g078505 27.857 280 155 8 503 751 252 515 6.41e-20 94.7
MS.gene82987.t1 MTR_4g132610 29.101 189 112 3 569 751 851 1023 6.27e-19 92.4
MS.gene82987.t1 MTR_5g098680 36.076 158 87 4 321 473 267 415 4.90e-16 82.8
MS.gene82987.t1 MTR_8g102580 36.076 158 87 4 321 473 261 409 1.04e-15 81.6
MS.gene82987.t1 MTR_7g445810 28.287 251 130 10 226 450 38 264 6.28e-13 70.5
MS.gene82987.t1 MTR_3g114910 33.333 153 88 4 321 473 187 325 1.40e-12 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82987.t1 AT4G13460 61.237 485 181 5 282 764 170 649 0.0 629
MS.gene82987.t1 AT4G13460 61.237 485 181 5 282 764 170 649 0.0 629
MS.gene82987.t1 AT2G33290 58.264 484 190 5 282 764 178 650 0.0 602
MS.gene82987.t1 AT2G33290 58.264 484 190 5 282 764 178 650 0.0 602
MS.gene82987.t1 AT2G33290 58.264 484 190 5 282 764 178 650 0.0 602
MS.gene82987.t1 AT2G33290 58.264 484 190 5 282 764 178 650 0.0 602
MS.gene82987.t1 AT5G04940 40.166 483 259 15 279 753 180 640 2.54e-98 319
MS.gene82987.t1 AT5G04940 40.166 483 259 15 279 753 180 640 2.54e-98 319
MS.gene82987.t1 AT1G73100 36.347 553 312 15 220 753 108 639 8.98e-96 312
MS.gene82987.t1 AT2G22740 36.820 478 275 8 285 751 298 759 4.45e-93 308
MS.gene82987.t1 AT2G22740 36.820 478 275 8 285 751 298 759 4.45e-93 308
MS.gene82987.t1 AT2G35160 34.857 525 291 13 236 751 245 727 8.18e-90 298
MS.gene82987.t1 AT2G35160 34.857 525 291 13 236 751 281 763 3.00e-89 298
MS.gene82987.t1 AT2G35160 34.857 525 291 13 236 751 281 763 3.00e-89 298
MS.gene82987.t1 AT2G35160 34.857 525 291 13 236 751 281 763 3.00e-89 298
MS.gene82987.t1 AT2G35160 34.857 525 291 13 236 751 281 763 3.00e-89 298
MS.gene82987.t1 AT5G13960 36.831 486 258 16 296 752 129 594 1.20e-83 278
MS.gene82987.t1 AT5G13960 37.313 469 246 15 313 752 11 460 5.03e-83 273
MS.gene82987.t1 AT1G17770 31.836 512 314 16 258 753 169 661 6.26e-72 249
MS.gene82987.t1 AT2G24740 36.078 510 285 18 253 753 247 724 6.32e-72 250
MS.gene82987.t1 AT5G47150 40.449 178 97 4 296 473 156 324 7.37e-28 115
MS.gene82987.t1 AT5G47160 40.351 171 92 5 300 469 246 407 3.25e-24 106
MS.gene82987.t1 AT5G47160 40.351 171 92 5 300 469 246 407 3.25e-24 106
MS.gene82987.t1 AT5G47160 40.351 171 92 5 300 469 246 407 3.25e-24 106
MS.gene82987.t1 AT2G23740 30.964 197 109 5 570 756 1184 1363 5.34e-22 102
MS.gene82987.t1 AT2G23740 30.964 197 109 5 570 756 1184 1363 5.34e-22 102
MS.gene82987.t1 AT2G23740 30.964 197 109 5 570 756 1184 1363 5.34e-22 102
MS.gene82987.t1 AT2G23740 30.964 197 109 5 570 756 1177 1356 5.42e-22 102
MS.gene82987.t1 AT2G05900 38.506 174 99 6 358 531 26 191 1.28e-19 90.9
MS.gene82987.t1 AT1G57820 36.047 172 86 6 317 473 264 426 1.21e-16 84.7
MS.gene82987.t1 AT1G57820 36.047 172 86 6 317 473 264 426 1.21e-16 84.7
MS.gene82987.t1 AT3G03750 30.918 207 115 6 548 752 143 323 3.45e-16 81.3
MS.gene82987.t1 AT1G57820 35.503 169 88 6 317 473 264 423 1.52e-15 81.3
MS.gene82987.t1 AT5G39550 34.302 172 89 6 317 473 249 411 1.73e-15 80.9
MS.gene82987.t1 AT2G23740 30.405 148 79 4 570 710 1184 1314 5.16e-15 80.1
MS.gene82987.t1 AT2G23740 30.405 148 79 4 570 710 1184 1314 5.16e-15 80.1
MS.gene82987.t1 AT1G66040 31.977 172 93 5 317 473 249 411 1.07e-14 78.6
MS.gene82987.t1 AT1G66050 31.977 172 93 5 317 473 249 411 1.12e-14 78.2

Find 231 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAAATCTCAACCTTAATT+TGG 0.115237 8.3:+31497634 MS.gene82987:CDS
GCTAGGGATATTAAGAAAAT+TGG 0.127502 8.3:+31498670 MS.gene82987:CDS
CCAAATTAAGGTTGAGATTT+AGG 0.131281 8.3:-31497634 None:intergenic
GCTACTAGTGTAATTGTTTC+AGG 0.143034 8.3:+31498304 MS.gene82987:CDS
CCCCATTCTACCCATCTATT+TGG 0.166114 8.3:-31499213 None:intergenic
CTATTGATATTTCAAATAAT+AGG 0.206413 8.3:+31498722 MS.gene82987:CDS
GCAAATTTGTGATCAGAAAT+TGG 0.219259 8.3:+31498411 MS.gene82987:CDS
ACATACTGAATGAATATATT+TGG 0.228005 8.3:-31499360 None:intergenic
GATGGATATGGTGGTGATTT+GGG 0.254601 8.3:-31497375 None:intergenic
AAGCAATCCATGTGGATTAT+AGG 0.255997 8.3:-31498932 None:intergenic
TAGGTCCTTGGACCTTATTC+AGG 0.261644 8.3:+31499089 MS.gene82987:CDS
CGATGGATATGGTGGTGATT+TGG 0.266979 8.3:-31497376 None:intergenic
CTAAATCTCAACCTTAATTT+GGG 0.267648 8.3:+31497635 MS.gene82987:CDS
GGTGAGAGCGGTGTGATTTA+AGG 0.267982 8.3:-31497348 None:intergenic
TCAGATTCGGGAGAGAAATT+AGG 0.279456 8.3:-31497479 None:intergenic
TGATTTAAGGTTGAAATTCT+AGG 0.282052 8.3:-31497335 None:intergenic
TGAGAGTGGGTGAGGAGAAT+AGG 0.282380 8.3:+31498008 MS.gene82987:CDS
TAGGTTGTTGTTGGGAATCT+TGG 0.291847 8.3:-31497460 None:intergenic
GAGAGTGGGTGAGGAGAATA+GGG 0.304281 8.3:+31498009 MS.gene82987:CDS
AATTGGTTGTTGAAGTTGAT+AGG 0.309699 8.3:+31497900 MS.gene82987:CDS
TATTGATATTTCAAATAATA+GGG 0.311494 8.3:+31498723 MS.gene82987:CDS
TGATCATCGGTTTCAGATTC+GGG 0.313526 8.3:-31497491 None:intergenic
GCAACACGGTTTCGACAATT+AGG 0.314745 8.3:-31499006 None:intergenic
GATATAGGAAAGTCTGGTTT+TGG 0.315495 8.3:+31498580 MS.gene82987:CDS
GTCGAGTTTGATGGCATATA+TGG 0.315529 8.3:+31497718 MS.gene82987:CDS
GAATGGGTTTCAAGGGTTAA+TGG 0.316851 8.3:-31497233 None:intergenic
TTGATCATCGGTTTCAGATT+CGG 0.324605 8.3:-31497492 None:intergenic
AATATAGCTTGGAAGTGTTT+AGG 0.327076 8.3:+31499043 MS.gene82987:CDS
AATGAATGAGGAGAAGAAAT+TGG 0.327732 8.3:+31497883 MS.gene82987:CDS
AGCAATCCATGTGGATTATA+GGG 0.332580 8.3:-31498931 None:intergenic
GACGCGAAGAAATTGTTAAA+AGG 0.332988 8.3:-31497437 None:intergenic
ATATATTTGGACTCGAACTA+TGG 0.334818 8.3:-31499347 None:intergenic
ATCTTGCTCGCCCACTCGTC+CGG 0.340319 8.3:-31499480 None:intergenic
TTAGGAGGACATGAGCAAAA+AGG 0.343616 8.3:-31498988 None:intergenic
AGAGAACTCTGCATAGATTA+TGG 0.345054 8.3:+31499453 MS.gene82987:CDS
AATGGGTTTCAAGGGTTAAT+GGG 0.348241 8.3:-31497232 None:intergenic
GGGAGACAATTAATGGAATA+AGG 0.348461 8.3:+31498053 MS.gene82987:CDS
ATATCATTAAATAGCCTAAC+TGG 0.350719 8.3:-31498754 None:intergenic
GTTAGTTGCTTGTTTGATAT+AGG 0.351027 8.3:+31498565 MS.gene82987:CDS
AGAAATTGTTAAAAGGTTCC+AGG 0.355471 8.3:-31497430 None:intergenic
CACAGACAGGTTGGGGAGTT+AGG 0.355710 8.3:+31499070 MS.gene82987:CDS
ATTGGATGAGGAGAAGATAT+TGG 0.356889 8.3:+31497955 MS.gene82987:CDS
CTAGTACATTTCCAAATAGA+TGG 0.359463 8.3:+31499202 MS.gene82987:CDS
GAAGGGAGTGCATCTACTTC+TGG 0.362995 8.3:+31498508 MS.gene82987:CDS
TAAAGGATCCAGCCTGAATA+AGG 0.368631 8.3:-31499101 None:intergenic
TTTGATGGCATATATGGTTA+TGG 0.371504 8.3:+31497724 MS.gene82987:CDS
ATTGTCTGAGGAAAGGAAAT+TGG 0.371576 8.3:+31497937 MS.gene82987:CDS
AAGTGATGTTCCGATGGATA+TGG 0.384008 8.3:-31497387 None:intergenic
ACCAGTATACTCGCAGATAA+AGG 0.385474 8.3:-31499118 None:intergenic
TATGGTGGTGATTTGGGGTG+GGG 0.386266 8.3:-31497369 None:intergenic
TTGTTAAAAGGTTCCAGGTT+TGG 0.388510 8.3:-31497425 None:intergenic
CGTAGTATCAACCACATTCA+TGG 0.388913 8.3:+31498079 MS.gene82987:CDS
CTAGGGATATTAAGAAAATT+GGG 0.394850 8.3:+31498671 MS.gene82987:CDS
TCGAAACCGTGTTGCACAAA+AGG 0.395320 8.3:+31499014 MS.gene82987:CDS
TGTTGTTTCATTGGAGAATA+CGG 0.399431 8.3:+31497592 MS.gene82987:CDS
TCCAAATAGATGGGTAGAAT+GGG 0.401426 8.3:+31499212 MS.gene82987:CDS
GAGAGAAATTAGGTTGTTGT+TGG 0.401584 8.3:-31497469 None:intergenic
TGCACTATGGGATTGAGGTT+AGG 0.405470 8.3:+31498464 MS.gene82987:CDS
ACCACATTCATGGTTATATG+AGG 0.405787 8.3:+31498089 MS.gene82987:CDS
TCAAAGAGTATGTCTTATAA+TGG 0.405858 8.3:+31498271 MS.gene82987:CDS
GATATGGTGGTGATTTGGGG+TGG 0.408728 8.3:-31497371 None:intergenic
TAGAATTTGGCGTATAGGTT+GGG 0.409698 8.3:-31497515 None:intergenic
TGTGGTAGAATTTGGCGTAT+AGG 0.411852 8.3:-31497520 None:intergenic
AGAAATAGAAGTAGAAGAAT+GGG 0.413089 8.3:-31497249 None:intergenic
GCACTATGGGATTGAGGTTA+GGG 0.416487 8.3:+31498465 MS.gene82987:CDS
TTCCAAATAGATGGGTAGAA+TGG 0.421060 8.3:+31499211 MS.gene82987:CDS
TTGTTTGATATAGGAAAGTC+TGG 0.421956 8.3:+31498574 MS.gene82987:CDS
TAGGTCTACACAGACAGGTT+GGG 0.422135 8.3:+31499062 MS.gene82987:CDS
GAGAAATAGAAGTAGAAGAA+TGG 0.422660 8.3:-31497250 None:intergenic
TGAAACAACAAAATCATTGT+TGG 0.424236 8.3:-31497579 None:intergenic
GGCAGAGATGAGAGTGGGTG+AGG 0.425042 8.3:+31498000 MS.gene82987:CDS
TCCTTTATCTGCGAGTATAC+TGG 0.426481 8.3:+31499117 MS.gene82987:CDS
AGGTTGTTGTTGGGAATCTT+GGG 0.427326 8.3:-31497459 None:intergenic
CATCTATTTGGAAATGTACT+AGG 0.428903 8.3:-31499201 None:intergenic
GAAACAAGAGTCGAGTTTGA+TGG 0.434269 8.3:+31497709 MS.gene82987:CDS
AAGTAGAAGAATGGGTTTCA+AGG 0.435832 8.3:-31497241 None:intergenic
TATAAGTTATTGAGAATTGA+GGG 0.436671 8.3:+31498613 MS.gene82987:CDS
GTAGAATTTGGCGTATAGGT+TGG 0.436679 8.3:-31497516 None:intergenic
TCTTTATTATGAGTATCTTC+CGG 0.438277 8.3:+31498795 MS.gene82987:CDS
ATTGGTTGTTGAAGTTGATA+GGG 0.446123 8.3:+31497901 MS.gene82987:CDS
TCCTTGGACCTTATTCAGGC+TGG 0.446886 8.3:+31499093 MS.gene82987:CDS
CGAAACCGTGTTGCACAAAA+GGG 0.448108 8.3:+31499015 MS.gene82987:CDS
AATTGAGGGGCAGGCGAAGA+TGG 0.448823 8.3:+31498627 MS.gene82987:CDS
AAACTCGACTCTTGTTTCTC+AGG 0.449003 8.3:-31497704 None:intergenic
AGGAGGGAATTGTGTGTGAC+TGG 0.450008 8.3:+31498211 MS.gene82987:CDS
ACCTAGTGGTTGTGAATGTA+AGG 0.454638 8.3:+31498858 MS.gene82987:CDS
GGAATAGGAACATGAGAAGA+AGG 0.457689 8.3:-31497275 None:intergenic
AGAGAAATTAGGTTGTTGTT+GGG 0.464968 8.3:-31497468 None:intergenic
ATATGGTGGTGATTTGGGGT+GGG 0.466003 8.3:-31497370 None:intergenic
TTGATGAGAAATACTAAGAT+CGG 0.467844 8.3:-31497309 None:intergenic
GATTTAAGGTTGAAATTCTA+GGG 0.470586 8.3:-31497334 None:intergenic
TAAGGTTGAGATTTAGGTCC+GGG 0.474895 8.3:-31497628 None:intergenic
TTGGAAAGGAGTATGCACTA+TGG 0.475190 8.3:+31498451 MS.gene82987:CDS
ACATCACCAACATAAATTCC+AGG 0.479414 8.3:-31498178 None:intergenic
TGATGAGAAATACTAAGATC+GGG 0.480139 8.3:-31497308 None:intergenic
GGTTTGTGTTTCATCAGAGT+GGG 0.481554 8.3:+31498833 MS.gene82987:CDS
AGTAGAAGAATGGGTTTCAA+GGG 0.482906 8.3:-31497240 None:intergenic
GGATATTAAGAAAATTGGGT+TGG 0.483689 8.3:+31498675 MS.gene82987:CDS
AGGATAGTTGGACCGATACC+TGG 0.485997 8.3:+31498160 MS.gene82987:CDS
TTCGCTTCGCGTGAGAATTG+AGG 0.488587 8.3:+31497850 MS.gene82987:CDS
TCTTGCTCGCCCACTCGTCC+GGG 0.490402 8.3:-31499479 None:intergenic
GGTTATGGAGAAGGAAAGCC+AGG 0.494082 8.3:+31497739 MS.gene82987:CDS
AATTGTTTCAGGTGGTTATG+AGG 0.495448 8.3:+31498315 MS.gene82987:CDS
TAATCTAAAGGAGGTATTGA+CGG 0.498715 8.3:-31499279 None:intergenic
ATAGGGAAAATGTTCCAGTT+AGG 0.499113 8.3:+31498740 MS.gene82987:CDS
TATCGTGATCAAAGGATAGT+TGG 0.501111 8.3:+31498148 MS.gene82987:CDS
GTATAAGTTATTGAGAATTG+AGG 0.501728 8.3:+31498612 MS.gene82987:CDS
CACTGTTCTCAAAGATGCTA+GGG 0.502270 8.3:+31498654 MS.gene82987:CDS
CCCTCACAAGTTCCATCACC+TGG 0.503770 8.3:-31497757 None:intergenic
TCATGTCATGTTATATGCAA+TGG 0.506033 8.3:+31499413 MS.gene82987:CDS
TCTATGCAGAGTTCTCTCAT+AGG 0.506224 8.3:-31499447 None:intergenic
GTCGGCTACCAACGTGGAGG+GGG 0.510887 8.3:+31498033 MS.gene82987:CDS
AAAGGGGCTCAAATATAGCT+TGG 0.511664 8.3:+31499032 MS.gene82987:CDS
GAATCAGCGATTTCGAGAAA+AGG 0.512004 8.3:-31497557 None:intergenic
AGGTCTACACAGACAGGTTG+GGG 0.513098 8.3:+31499063 MS.gene82987:CDS
GATTTGGGGTGGGGTGAGAG+CGG 0.514925 8.3:-31497360 None:intergenic
GGGGTGGATTCTGTAAATGA+TGG 0.515279 8.3:+31497656 MS.gene82987:CDS
ACTCGAACTATGGCTCATAT+AGG 0.515301 8.3:-31499337 None:intergenic
TTAAGGTTGAGATTTAGGTC+CGG 0.515596 8.3:-31497629 None:intergenic
GAGTGCATCTACTTCTGGTA+AGG 0.518621 8.3:+31498513 MS.gene82987:CDS
GTATAGGTTGGGTTGATCAT+CGG 0.521132 8.3:-31497504 None:intergenic
TCAATAGAGATACAACTCAT+TGG 0.521177 8.3:-31498706 None:intergenic
TGCACTTTCGTCATAAGATA+AGG 0.521451 8.3:+31497807 MS.gene82987:CDS
GTGTCGGCTACCAACGTGGA+GGG 0.521469 8.3:+31498031 MS.gene82987:CDS
GCACTGTTCTCAAAGATGCT+AGG 0.522783 8.3:+31498653 MS.gene82987:CDS
TGTCGGCTACCAACGTGGAG+GGG 0.522877 8.3:+31498032 MS.gene82987:CDS
GCATACTCCTTTCCAATGCA+AGG 0.525232 8.3:-31498444 None:intergenic
GGCATATATGGTTATGGAGA+AGG 0.527217 8.3:+31497730 MS.gene82987:CDS
GGAGGTATTGACGGATAAGA+TGG 0.528649 8.3:-31499270 None:intergenic
TAATGAAGGAGCGTGGGCTA+TGG 0.529339 8.3:+31498122 MS.gene82987:CDS
AATCAGCGATTTCGAGAAAA+GGG 0.530354 8.3:-31497556 None:intergenic
AGAAGAGATTGTCTGAGGAA+AGG 0.532258 8.3:+31497930 MS.gene82987:CDS
GGAGAAGGAAAGCCAGGTGA+TGG 0.532924 8.3:+31497745 MS.gene82987:CDS
TGGTTTGTGTTTCATCAGAG+TGG 0.533225 8.3:+31498832 MS.gene82987:CDS
ATTATAATCTACACTGGCCA+TGG 0.534081 8.3:+31498364 MS.gene82987:CDS
TACTGGTGTTGCTATAACTA+GGG 0.536645 8.3:+31499134 MS.gene82987:CDS
AACAATGAATGGGGTCTCGT+TGG 0.538408 8.3:+31499173 MS.gene82987:CDS
GTTATTGAGAATTGAGGGGC+AGG 0.539794 8.3:+31498618 MS.gene82987:CDS
ACAATTACACTAGTAGCAAT+CGG 0.540891 8.3:-31498298 None:intergenic
TGGAAAGGAGTATGCACTAT+GGG 0.541585 8.3:+31498452 MS.gene82987:CDS
AATTCTTCTCTCGCCCTCCA+TGG 0.544307 8.3:-31498381 None:intergenic
GGTTGTTGAAGTTGATAGGG+AGG 0.545333 8.3:+31497904 MS.gene82987:CDS
ACATCCAGAAAATAATCTAA+AGG 0.546485 8.3:-31499291 None:intergenic
CTGATCACAAATTTGCCTTG+AGG 0.547153 8.3:-31498404 None:intergenic
ATTGAGGGGCAGGCGAAGAT+GGG 0.548419 8.3:+31498628 MS.gene82987:CDS
ATACTGGTGTTGCTATAACT+AGG 0.549242 8.3:+31499133 MS.gene82987:CDS
AACGCCACTCATTCCAAACC+TGG 0.552250 8.3:+31497412 MS.gene82987:CDS
GAGTATGCACTATGGGATTG+AGG 0.552471 8.3:+31498459 MS.gene82987:CDS
CCAGGTGATGGAACTTGTGA+GGG 0.555665 8.3:+31497757 MS.gene82987:CDS
ATGCAGAGTTCTCTCATAGG+AGG 0.558353 8.3:-31499444 None:intergenic
GGAGGGTGGAAACCTTGCAT+TGG 0.558530 8.3:+31498432 MS.gene82987:CDS
CTCTGATGAAACACAAACCA+CGG 0.558774 8.3:-31498829 None:intergenic
GGAAGATACTCATAATAAAG+AGG 0.560347 8.3:-31498793 None:intergenic
GGTAAGGTTTATGTTTACGA+TGG 0.560574 8.3:+31498529 MS.gene82987:CDS
GTTAATGGGAAAGGATCCAT+TGG 0.561622 8.3:-31497218 None:intergenic
TGAGGAAAGGAAATTGGATG+AGG 0.567529 8.3:+31497943 MS.gene82987:CDS
ATTCGCGGGATGAAGTATGA+AGG 0.568414 8.3:+31498490 MS.gene82987:CDS
GGAGGACAAGAGAATGAATG+AGG 0.571585 8.3:+31497871 MS.gene82987:CDS
GGAAGAGAAGAGATTGTCTG+AGG 0.572868 8.3:+31497925 MS.gene82987:CDS
AACCACGGGAAAGTAGTATC+CGG 0.574690 8.3:-31498814 None:intergenic
CCGATACCTGGAATTTATGT+TGG 0.577931 8.3:+31498172 MS.gene82987:CDS
ATTGAGGTTAGGGTTATTCG+CGG 0.577972 8.3:+31498475 MS.gene82987:CDS
GGTTCCAGGTTTGGAATGAG+TGG 0.578526 8.3:-31497416 None:intergenic
GGTTTCAGATCTGAGTATGA+AGG 0.579176 8.3:+31497778 MS.gene82987:CDS
GTGGAAACCTTGCATTGGAA+AGG 0.581268 8.3:+31498437 MS.gene82987:CDS
ATGGATATGGTGGTGATTTG+GGG 0.583200 8.3:-31497374 None:intergenic
CGACATTAATGAAGGAGCGT+GGG 0.584881 8.3:+31498116 MS.gene82987:CDS
TAGTACATTTCCAAATAGAT+GGG 0.585798 8.3:+31499203 MS.gene82987:CDS
GAGGGCTGCGACATTAATGA+AGG 0.586703 8.3:+31498108 MS.gene82987:CDS
CTGCATAGATTATGGCATCC+CGG 0.587677 8.3:+31499461 MS.gene82987:CDS
GGATGCAGAAGTTGCAGTTG+TGG 0.588062 8.3:+31497976 MS.gene82987:CDS
CCAACATAAATTCCAGGTAT+CGG 0.588388 8.3:-31498172 None:intergenic
TAAATCTCAACCTTAATTTG+GGG 0.590545 8.3:+31497636 MS.gene82987:CDS
GTGTTTAGGTCTACACAGAC+AGG 0.591190 8.3:+31499057 MS.gene82987:CDS
TATGGTTGTATCGTGATCAA+AGG 0.592056 8.3:+31498140 MS.gene82987:CDS
TGTGATCAGAAATTGGAGGG+TGG 0.592511 8.3:+31498418 MS.gene82987:CDS
GTTTCAAGGGTTAATGGGAA+AGG 0.597617 8.3:-31497227 None:intergenic
GCCAGGTGATGGAACTTGTG+AGG 0.598287 8.3:+31497756 MS.gene82987:CDS
TGCAGAGTTCTCTCATAGGA+GGG 0.599457 8.3:-31499443 None:intergenic
TAATCTACACTGGCCATGGA+GGG 0.600090 8.3:+31498368 MS.gene82987:CDS
ACACGGTTTCGACAATTAGG+AGG 0.600465 8.3:-31499003 None:intergenic
ACTAGTGTAATTGTTTCAGG+TGG 0.601921 8.3:+31498307 MS.gene82987:CDS
TACGGTAAATGTTTGCAACC+CGG 0.602140 8.3:+31497610 MS.gene82987:CDS
AGGTTGGGGAGTTAGGTCCT+TGG 0.602845 8.3:+31499077 MS.gene82987:CDS
AGGTGGTTATGAGGATGACA+TGG 0.604358 8.3:+31498324 MS.gene82987:CDS
GGTGATATTATAATCTACAC+TGG 0.606082 8.3:+31498358 MS.gene82987:CDS
GTGGAGGCAGAGATGAGAGT+GGG 0.608465 8.3:+31497995 MS.gene82987:CDS
CCACATTCATGGTTATATGA+GGG 0.608512 8.3:+31498090 MS.gene82987:CDS
TTCGTCATAAGATAAGGAAG+AGG 0.608918 8.3:+31497813 MS.gene82987:CDS
ATTGCATATAACATGACATG+AGG 0.609949 8.3:-31499411 None:intergenic
GCGACATTAATGAAGGAGCG+TGG 0.612288 8.3:+31498115 MS.gene82987:CDS
ATTATGGCATCCCGGACGAG+TGG 0.613316 8.3:+31499469 MS.gene82987:CDS
TCTGATGAAACACAAACCAC+GGG 0.613935 8.3:-31498828 None:intergenic
ATTTGTGATCAGAAATTGGA+GGG 0.614502 8.3:+31498415 MS.gene82987:CDS
TTGCATATGCATATGCAAGC+TGG 0.615698 8.3:+31498235 MS.gene82987:CDS
CGTTTGAAGTGATGTTCCGA+TGG 0.617924 8.3:-31497393 None:intergenic
GTTTGTGTTTCATCAGAGTG+GGG 0.619286 8.3:+31498834 MS.gene82987:CDS
GAGTTTCCCTATAATCCACA+TGG 0.620559 8.3:+31498925 MS.gene82987:CDS
CCCTCATATAACCATGAATG+TGG 0.621384 8.3:-31498090 None:intergenic
GGGTGAGGAGAATAGGGTGT+CGG 0.621767 8.3:+31498015 MS.gene82987:CDS
TCCAGCCTGAATAAGGTCCA+AGG 0.622985 8.3:-31499094 None:intergenic
ATAAGTTATTGAGAATTGAG+GGG 0.623555 8.3:+31498614 MS.gene82987:CDS
TTCGCGGGATGAAGTATGAA+GGG 0.624136 8.3:+31498491 MS.gene82987:CDS
TTATGGCATCCCGGACGAGT+GGG 0.624731 8.3:+31499470 MS.gene82987:CDS
CAAATCACCACCATATCCAT+CGG 0.625118 8.3:+31497377 MS.gene82987:CDS
ATAATCTACACTGGCCATGG+AGG 0.626398 8.3:+31498367 MS.gene82987:CDS
TTGAGGTTAGGGTTATTCGC+GGG 0.628964 8.3:+31498476 MS.gene82987:CDS
GGTGTCGGCTACCAACGTGG+AGG 0.632053 8.3:+31498030 MS.gene82987:CDS
TCCAGAAAATAATCTAAAGG+AGG 0.632885 8.3:-31499288 None:intergenic
TTAGGTCTACACAGACAGGT+TGG 0.633420 8.3:+31499061 MS.gene82987:CDS
TGTAAGGAGGCTTGTACTGA+CGG 0.637592 8.3:+31498874 MS.gene82987:CDS
TGCAGAAGTTGCAGTTGTGG+AGG 0.639345 8.3:+31497979 MS.gene82987:CDS
GATGAGAAATACTAAGATCG+GGG 0.640700 8.3:-31497307 None:intergenic
CCTTTCAAAAGCAATCCATG+TGG 0.644916 8.3:-31498940 None:intergenic
GCTTCGCGTGAGAATTGAGG+AGG 0.645275 8.3:+31497853 MS.gene82987:CDS
AAATCTCAACCTTAATTTGG+GGG 0.645557 8.3:+31497637 MS.gene82987:CDS
TTCCGGATACTACTTTCCCG+TGG 0.649132 8.3:+31498812 MS.gene82987:CDS
CCAAATAGATGGGTAGAATG+GGG 0.657480 8.3:+31499213 MS.gene82987:CDS
TCCTTACATTCACAACCACT+AGG 0.658950 8.3:-31498859 None:intergenic
CATCAGAGTGGGGAACCTAG+TGG 0.659288 8.3:+31498844 MS.gene82987:CDS
TTGCATATAACATGACATGA+GGG 0.662482 8.3:-31499410 None:intergenic
GGCGAGAGAAGAATTCCTCA+AGG 0.664502 8.3:+31498389 MS.gene82987:CDS
GAAACCGTGTTGCACAAAAG+GGG 0.665958 8.3:+31499016 MS.gene82987:CDS
TGATGTTCCGATGGATATGG+TGG 0.669643 8.3:-31497384 None:intergenic
ATGAGAAATACTAAGATCGG+GGG 0.677362 8.3:-31497306 None:intergenic
TAGTGGTTGTGAATGTAAGG+AGG 0.682180 8.3:+31498861 MS.gene82987:CDS
TAGGGTGTCGGCTACCAACG+TGG 0.694009 8.3:+31498027 MS.gene82987:CDS
AATTTGTGATCAGAAATTGG+AGG 0.699714 8.3:+31498414 MS.gene82987:CDS
TGTGGAGGCAGAGATGAGAG+TGG 0.704475 8.3:+31497994 MS.gene82987:CDS
AAATGATCGAGCTATTGTTG+TGG 0.743063 8.3:+31497679 MS.gene82987:CDS
GATGACATGGAATTAGACGA+TGG 0.783210 8.3:+31498337 MS.gene82987:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGATATTTCAAATAATA+GGG + chr8.3:31498723-31498742 MS.gene82987:CDS 10.0%
!! CTATTGATATTTCAAATAAT+AGG + chr8.3:31498722-31498741 MS.gene82987:CDS 15.0%
!! ACATACTGAATGAATATATT+TGG - chr8.3:31499363-31499382 None:intergenic 20.0%
!! TATAAGTTATTGAGAATTGA+GGG + chr8.3:31498613-31498632 MS.gene82987:CDS 20.0%
!!! CTCAAATTTTAACAATGAAT+GGG + chr8.3:31499163-31499182 MS.gene82987:CDS 20.0%
!!! TCAAATTTTAACAATGAATG+GGG + chr8.3:31499164-31499183 MS.gene82987:CDS 20.0%
! ACATCCAGAAAATAATCTAA+AGG - chr8.3:31499294-31499313 None:intergenic 25.0%
! AGAAATAGAAGTAGAAGAAT+GGG - chr8.3:31497252-31497271 None:intergenic 25.0%
! ATAAGTTATTGAGAATTGAG+GGG + chr8.3:31498614-31498633 MS.gene82987:CDS 25.0%
! ATATCATTAAATAGCCTAAC+TGG - chr8.3:31498757-31498776 None:intergenic 25.0%
! CTAAATCTCAACCTTAATTT+GGG + chr8.3:31497635-31497654 MS.gene82987:CDS 25.0%
! CTAGGGATATTAAGAAAATT+GGG + chr8.3:31498671-31498690 MS.gene82987:CDS 25.0%
! GTATAAGTTATTGAGAATTG+AGG + chr8.3:31498612-31498631 MS.gene82987:CDS 25.0%
! TAGTACATTTCCAAATAGAT+GGG + chr8.3:31499203-31499222 MS.gene82987:CDS 25.0%
! TCTTTATTATGAGTATCTTC+CGG + chr8.3:31498795-31498814 MS.gene82987:CDS 25.0%
! TGAAACAACAAAATCATTGT+TGG - chr8.3:31497582-31497601 None:intergenic 25.0%
! TTGATGAGAAATACTAAGAT+CGG - chr8.3:31497312-31497331 None:intergenic 25.0%
!! GATTTAAGGTTGAAATTCTA+GGG - chr8.3:31497337-31497356 None:intergenic 25.0%
!! TAAATCTCAACCTTAATTTG+GGG + chr8.3:31497636-31497655 MS.gene82987:CDS 25.0%
!! TCAAAGAGTATGTCTTATAA+TGG + chr8.3:31498271-31498290 MS.gene82987:CDS 25.0%
!! TGATTTAAGGTTGAAATTCT+AGG - chr8.3:31497338-31497357 None:intergenic 25.0%
!!! AAACCTTTGATTTTTGAATG+CGG + chr8.3:31498964-31498983 MS.gene82987:CDS 25.0%
!!! CAATGATTTTGTTGTTTCAT+TGG + chr8.3:31497583-31497602 MS.gene82987:CDS 25.0%
!!! GCTCAAATTTTAACAATGAA+TGG + chr8.3:31499162-31499181 MS.gene82987:CDS 25.0%
AATGAATGAGGAGAAGAAAT+TGG + chr8.3:31497883-31497902 MS.gene82987:CDS 30.0%
AATTTGTGATCAGAAATTGG+AGG + chr8.3:31498414-31498433 MS.gene82987:CDS 30.0%
ACAATTACACTAGTAGCAAT+CGG - chr8.3:31498301-31498320 None:intergenic 30.0%
AGAAATTGTTAAAAGGTTCC+AGG - chr8.3:31497433-31497452 None:intergenic 30.0%
ATATATTTGGACTCGAACTA+TGG - chr8.3:31499350-31499369 None:intergenic 30.0%
ATTGCATATAACATGACATG+AGG - chr8.3:31499414-31499433 None:intergenic 30.0%
ATTTGTGATCAGAAATTGGA+GGG + chr8.3:31498415-31498434 MS.gene82987:CDS 30.0%
CATCTATTTGGAAATGTACT+AGG - chr8.3:31499204-31499223 None:intergenic 30.0%
CCAAATTAAGGTTGAGATTT+AGG - chr8.3:31497637-31497656 None:intergenic 30.0%
CCTAAATCTCAACCTTAATT+TGG + chr8.3:31497634-31497653 MS.gene82987:CDS 30.0%
CTAGTACATTTCCAAATAGA+TGG + chr8.3:31499202-31499221 MS.gene82987:CDS 30.0%
GAGAAATAGAAGTAGAAGAA+TGG - chr8.3:31497253-31497272 None:intergenic 30.0%
GCAAATTTGTGATCAGAAAT+TGG + chr8.3:31498411-31498430 MS.gene82987:CDS 30.0%
GCTAGGGATATTAAGAAAAT+TGG + chr8.3:31498670-31498689 MS.gene82987:CDS 30.0%
GGAAGATACTCATAATAAAG+AGG - chr8.3:31498796-31498815 None:intergenic 30.0%
GGATATTAAGAAAATTGGGT+TGG + chr8.3:31498675-31498694 MS.gene82987:CDS 30.0%
GGTGATATTATAATCTACAC+TGG + chr8.3:31498358-31498377 MS.gene82987:CDS 30.0%
GTTAGTTGCTTGTTTGATAT+AGG + chr8.3:31498565-31498584 MS.gene82987:CDS 30.0%
TCAATAGAGATACAACTCAT+TGG - chr8.3:31498709-31498728 None:intergenic 30.0%
TCATGTCATGTTATATGCAA+TGG + chr8.3:31499413-31499432 MS.gene82987:CDS 30.0%
TCCAGAAAATAATCTAAAGG+AGG - chr8.3:31499291-31499310 None:intergenic 30.0%
TGATGAGAAATACTAAGATC+GGG - chr8.3:31497311-31497330 None:intergenic 30.0%
TTGCATATAACATGACATGA+GGG - chr8.3:31499413-31499432 None:intergenic 30.0%
TTGTTTGATATAGGAAAGTC+TGG + chr8.3:31498574-31498593 MS.gene82987:CDS 30.0%
! AAATCTCAACCTTAATTTGG+GGG + chr8.3:31497637-31497656 MS.gene82987:CDS 30.0%
! AATTGGTTGTTGAAGTTGAT+AGG + chr8.3:31497900-31497919 MS.gene82987:CDS 30.0%
! ACCTCCTTTAGATTATTTTC+TGG + chr8.3:31499287-31499306 MS.gene82987:CDS 30.0%
! AGAGAAATTAGGTTGTTGTT+GGG - chr8.3:31497471-31497490 None:intergenic 30.0%
! TTTGATGGCATATATGGTTA+TGG + chr8.3:31497724-31497743 MS.gene82987:CDS 30.0%
!! AATATAGCTTGGAAGTGTTT+AGG + chr8.3:31499043-31499062 MS.gene82987:CDS 30.0%
!! ATTGGTTGTTGAAGTTGATA+GGG + chr8.3:31497901-31497920 MS.gene82987:CDS 30.0%
!! TAATCTAAAGGAGGTATTGA+CGG - chr8.3:31499282-31499301 None:intergenic 30.0%
!! TGATGTGTTTTTGTTTAGGA+GGG + chr8.3:31498195-31498214 MS.gene82987:CDS 30.0%
!! TGTTGTTTCATTGGAGAATA+CGG + chr8.3:31497592-31497611 MS.gene82987:CDS 30.0%
!!! TTGGTGATGTGTTTTTGTTT+AGG + chr8.3:31498191-31498210 MS.gene82987:CDS 30.0%
AAATGATCGAGCTATTGTTG+TGG + chr8.3:31497679-31497698 MS.gene82987:CDS 35.0%
AATCAGCGATTTCGAGAAAA+GGG - chr8.3:31497559-31497578 None:intergenic 35.0%
ACATCACCAACATAAATTCC+AGG - chr8.3:31498181-31498200 None:intergenic 35.0%
ACCACATTCATGGTTATATG+AGG + chr8.3:31498089-31498108 MS.gene82987:CDS 35.0%
ACTAGTGTAATTGTTTCAGG+TGG + chr8.3:31498307-31498326 MS.gene82987:CDS 35.0%
AGAGAACTCTGCATAGATTA+TGG + chr8.3:31499453-31499472 MS.gene82987:CDS 35.0%
ATAGGGAAAATGTTCCAGTT+AGG + chr8.3:31498740-31498759 MS.gene82987:CDS 35.0%
ATGAGAAATACTAAGATCGG+GGG - chr8.3:31497309-31497328 None:intergenic 35.0%
ATTATAATCTACACTGGCCA+TGG + chr8.3:31498364-31498383 MS.gene82987:CDS 35.0%
ATTGGATGAGGAGAAGATAT+TGG + chr8.3:31497955-31497974 MS.gene82987:CDS 35.0%
ATTGTCTGAGGAAAGGAAAT+TGG + chr8.3:31497937-31497956 MS.gene82987:CDS 35.0%
CCAACATAAATTCCAGGTAT+CGG - chr8.3:31498175-31498194 None:intergenic 35.0%
CCACATTCATGGTTATATGA+GGG + chr8.3:31498090-31498109 MS.gene82987:CDS 35.0%
GACGCGAAGAAATTGTTAAA+AGG - chr8.3:31497440-31497459 None:intergenic 35.0%
GATGAGAAATACTAAGATCG+GGG - chr8.3:31497310-31497329 None:intergenic 35.0%
GCTACTAGTGTAATTGTTTC+AGG + chr8.3:31498304-31498323 MS.gene82987:CDS 35.0%
GGGAGACAATTAATGGAATA+AGG + chr8.3:31498053-31498072 MS.gene82987:CDS 35.0%
GGTAAGGTTTATGTTTACGA+TGG + chr8.3:31498529-31498548 MS.gene82987:CDS 35.0%
TAGAATTTGGCGTATAGGTT+GGG - chr8.3:31497518-31497537 None:intergenic 35.0%
TATCGTGATCAAAGGATAGT+TGG + chr8.3:31498148-31498167 MS.gene82987:CDS 35.0%
TATGGTTGTATCGTGATCAA+AGG + chr8.3:31498140-31498159 MS.gene82987:CDS 35.0%
TCCAAATAGATGGGTAGAAT+GGG + chr8.3:31499212-31499231 MS.gene82987:CDS 35.0%
TGCACTTTCGTCATAAGATA+AGG + chr8.3:31497807-31497826 MS.gene82987:CDS 35.0%
TTAAGGTTGAGATTTAGGTC+CGG - chr8.3:31497632-31497651 None:intergenic 35.0%
TTCCAAATAGATGGGTAGAA+TGG + chr8.3:31499211-31499230 MS.gene82987:CDS 35.0%
TTCGTCATAAGATAAGGAAG+AGG + chr8.3:31497813-31497832 MS.gene82987:CDS 35.0%
TTGTTAAAAGGTTCCAGGTT+TGG - chr8.3:31497428-31497447 None:intergenic 35.0%
! AAGCAATCCATGTGGATTAT+AGG - chr8.3:31498935-31498954 None:intergenic 35.0%
! AAGTAGAAGAATGGGTTTCA+AGG - chr8.3:31497244-31497263 None:intergenic 35.0%
! AATGGGTTTCAAGGGTTAAT+GGG - chr8.3:31497235-31497254 None:intergenic 35.0%
! AATTGTTTCAGGTGGTTATG+AGG + chr8.3:31498315-31498334 MS.gene82987:CDS 35.0%
! AGCAATCCATGTGGATTATA+GGG - chr8.3:31498934-31498953 None:intergenic 35.0%
! AGTAGAAGAATGGGTTTCAA+GGG - chr8.3:31497243-31497262 None:intergenic 35.0%
! GAGAGAAATTAGGTTGTTGT+TGG - chr8.3:31497472-31497491 None:intergenic 35.0%
!! ATACTGGTGTTGCTATAACT+AGG + chr8.3:31499133-31499152 MS.gene82987:CDS 35.0%
!! CACATGGATTGCTTTTGAAA+GGG + chr8.3:31498941-31498960 MS.gene82987:CDS 35.0%
!! GATATAGGAAAGTCTGGTTT+TGG + chr8.3:31498580-31498599 MS.gene82987:CDS 35.0%
!! GTGATGTGTTTTTGTTTAGG+AGG + chr8.3:31498194-31498213 MS.gene82987:CDS 35.0%
!! TACTGGTGTTGCTATAACTA+GGG + chr8.3:31499134-31499153 MS.gene82987:CDS 35.0%
!! TTGATCATCGGTTTCAGATT+CGG - chr8.3:31497495-31497514 None:intergenic 35.0%
!!! TTTTGAGGTGTGGTAGAATT+TGG - chr8.3:31497531-31497550 None:intergenic 35.0%
AAAGGGGCTCAAATATAGCT+TGG + chr8.3:31499032-31499051 MS.gene82987:CDS 40.0%
AAGTGATGTTCCGATGGATA+TGG - chr8.3:31497390-31497409 None:intergenic 40.0%
ACCAGTATACTCGCAGATAA+AGG - chr8.3:31499121-31499140 None:intergenic 40.0%
ACCTAGTGGTTGTGAATGTA+AGG + chr8.3:31498858-31498877 MS.gene82987:CDS 40.0%
ACTCGAACTATGGCTCATAT+AGG - chr8.3:31499340-31499359 None:intergenic 40.0%
AGAAGAGATTGTCTGAGGAA+AGG + chr8.3:31497930-31497949 MS.gene82987:CDS 40.0%
ATTGAGGTTAGGGTTATTCG+CGG + chr8.3:31498475-31498494 MS.gene82987:CDS 40.0%
CAAATCACCACCATATCCAT+CGG + chr8.3:31497377-31497396 MS.gene82987:CDS 40.0%
CACTGTTCTCAAAGATGCTA+GGG + chr8.3:31498654-31498673 MS.gene82987:CDS 40.0%
CCAAATAGATGGGTAGAATG+GGG + chr8.3:31499213-31499232 MS.gene82987:CDS 40.0%
CCCTCATATAACCATGAATG+TGG - chr8.3:31498093-31498112 None:intergenic 40.0%
CCGATACCTGGAATTTATGT+TGG + chr8.3:31498172-31498191 MS.gene82987:CDS 40.0%
CCTTTCAAAAGCAATCCATG+TGG - chr8.3:31498943-31498962 None:intergenic 40.0%
CGTAGTATCAACCACATTCA+TGG + chr8.3:31498079-31498098 MS.gene82987:CDS 40.0%
CTCTGATGAAACACAAACCA+CGG - chr8.3:31498832-31498851 None:intergenic 40.0%
CTGATCACAAATTTGCCTTG+AGG - chr8.3:31498407-31498426 None:intergenic 40.0%
GAAACAAGAGTCGAGTTTGA+TGG + chr8.3:31497709-31497728 MS.gene82987:CDS 40.0%
GAATCAGCGATTTCGAGAAA+AGG - chr8.3:31497560-31497579 None:intergenic 40.0%
GAGTTTCCCTATAATCCACA+TGG + chr8.3:31498925-31498944 MS.gene82987:CDS 40.0%
GATGACATGGAATTAGACGA+TGG + chr8.3:31498337-31498356 MS.gene82987:CDS 40.0%
GGAATAGGAACATGAGAAGA+AGG - chr8.3:31497278-31497297 None:intergenic 40.0%
GGCATATATGGTTATGGAGA+AGG + chr8.3:31497730-31497749 MS.gene82987:CDS 40.0%
GGTTTCAGATCTGAGTATGA+AGG + chr8.3:31497778-31497797 MS.gene82987:CDS 40.0%
GGTTTGTGTTTCATCAGAGT+GGG + chr8.3:31498833-31498852 MS.gene82987:CDS 40.0%
GTAGAATTTGGCGTATAGGT+TGG - chr8.3:31497519-31497538 None:intergenic 40.0%
GTTTCAAGGGTTAATGGGAA+AGG - chr8.3:31497230-31497249 None:intergenic 40.0%
GTTTGTGTTTCATCAGAGTG+GGG + chr8.3:31498834-31498853 MS.gene82987:CDS 40.0%
TAAAGGATCCAGCCTGAATA+AGG - chr8.3:31499104-31499123 None:intergenic 40.0%
TAAGGTTGAGATTTAGGTCC+GGG - chr8.3:31497631-31497650 None:intergenic 40.0%
TACGGTAAATGTTTGCAACC+CGG + chr8.3:31497610-31497629 MS.gene82987:CDS 40.0%
TAGGAGGACATGAGCAAAAA+GGG - chr8.3:31498990-31499009 None:intergenic 40.0%
TAGTGGTTGTGAATGTAAGG+AGG + chr8.3:31498861-31498880 MS.gene82987:CDS 40.0%
TCAGATTCGGGAGAGAAATT+AGG - chr8.3:31497482-31497501 None:intergenic 40.0%
TCCTTACATTCACAACCACT+AGG - chr8.3:31498862-31498881 None:intergenic 40.0%
TCCTTTATCTGCGAGTATAC+TGG + chr8.3:31499117-31499136 MS.gene82987:CDS 40.0%
TCTATGCAGAGTTCTCTCAT+AGG - chr8.3:31499450-31499469 None:intergenic 40.0%
TCTGATGAAACACAAACCAC+GGG - chr8.3:31498831-31498850 None:intergenic 40.0%
TGAGGAAAGGAAATTGGATG+AGG + chr8.3:31497943-31497962 MS.gene82987:CDS 40.0%
TGTGGTAGAATTTGGCGTAT+AGG - chr8.3:31497523-31497542 None:intergenic 40.0%
TTAGGAGGACATGAGCAAAA+AGG - chr8.3:31498991-31499010 None:intergenic 40.0%
TTGCATATGCATATGCAAGC+TGG + chr8.3:31498235-31498254 MS.gene82987:CDS 40.0%
! AAACTCGACTCTTGTTTCTC+AGG - chr8.3:31497707-31497726 None:intergenic 40.0%
! AGGTTGTTGTTGGGAATCTT+GGG - chr8.3:31497462-31497481 None:intergenic 40.0%
! ATGGATATGGTGGTGATTTG+GGG - chr8.3:31497377-31497396 None:intergenic 40.0%
! GAATGGGTTTCAAGGGTTAA+TGG - chr8.3:31497236-31497255 None:intergenic 40.0%
! GAGAAAAGGGTAGAGTTTTG+AGG - chr8.3:31497546-31497565 None:intergenic 40.0%
! GATGGATATGGTGGTGATTT+GGG - chr8.3:31497378-31497397 None:intergenic 40.0%
! GTCGAGTTTGATGGCATATA+TGG + chr8.3:31497718-31497737 MS.gene82987:CDS 40.0%
! TAGGTTGTTGTTGGGAATCT+TGG - chr8.3:31497463-31497482 None:intergenic 40.0%
! TGCTTTTGCGCTATGAAGAA+TGG + chr8.3:31498898-31498917 MS.gene82987:CDS 40.0%
! TGGAAAGGAGTATGCACTAT+GGG + chr8.3:31498452-31498471 MS.gene82987:CDS 40.0%
! TGGTTTGTGTTTCATCAGAG+TGG + chr8.3:31498832-31498851 MS.gene82987:CDS 40.0%
! TTGGAAAGGAGTATGCACTA+TGG + chr8.3:31498451-31498470 MS.gene82987:CDS 40.0%
! TTTTGCGCTATGAAGAATGG+AGG + chr8.3:31498901-31498920 MS.gene82987:CDS 40.0%
!! CCACATGGATTGCTTTTGAA+AGG + chr8.3:31498940-31498959 MS.gene82987:CDS 40.0%
!! GTATAGGTTGGGTTGATCAT+CGG - chr8.3:31497507-31497526 None:intergenic 40.0%
!! TGATCATCGGTTTCAGATTC+GGG - chr8.3:31497494-31497513 None:intergenic 40.0%
AACAATGAATGGGGTCTCGT+TGG + chr8.3:31499173-31499192 MS.gene82987:CDS 45.0%
AACCACGGGAAAGTAGTATC+CGG - chr8.3:31498817-31498836 None:intergenic 45.0%
ACACGGTTTCGACAATTAGG+AGG - chr8.3:31499006-31499025 None:intergenic 45.0%
AGGTGGTTATGAGGATGACA+TGG + chr8.3:31498324-31498343 MS.gene82987:CDS 45.0%
ATAATCTACACTGGCCATGG+AGG + chr8.3:31498367-31498386 MS.gene82987:CDS 45.0%
ATGCAGAGTTCTCTCATAGG+AGG - chr8.3:31499447-31499466 None:intergenic 45.0%
ATTCGCGGGATGAAGTATGA+AGG + chr8.3:31498490-31498509 MS.gene82987:CDS 45.0%
CAGAATCCACCCCCAAATTA+AGG - chr8.3:31497649-31497668 None:intergenic 45.0%
CCCCATTCTACCCATCTATT+TGG - chr8.3:31499216-31499235 None:intergenic 45.0%
CGAAACCGTGTTGCACAAAA+GGG + chr8.3:31499015-31499034 MS.gene82987:CDS 45.0%
CTGCATAGATTATGGCATCC+CGG + chr8.3:31499461-31499480 MS.gene82987:CDS 45.0%
GAAACCGTGTTGCACAAAAG+GGG + chr8.3:31499016-31499035 MS.gene82987:CDS 45.0%
GAGTATGCACTATGGGATTG+AGG + chr8.3:31498459-31498478 MS.gene82987:CDS 45.0%
GAGTGCATCTACTTCTGGTA+AGG + chr8.3:31498513-31498532 MS.gene82987:CDS 45.0%
GCAACACGGTTTCGACAATT+AGG - chr8.3:31499009-31499028 None:intergenic 45.0%
GCACTATGGGATTGAGGTTA+GGG + chr8.3:31498465-31498484 MS.gene82987:CDS 45.0%
GCACTGTTCTCAAAGATGCT+AGG + chr8.3:31498653-31498672 MS.gene82987:CDS 45.0%
GCATACTCCTTTCCAATGCA+AGG - chr8.3:31498447-31498466 None:intergenic 45.0%
GGAAGAGAAGAGATTGTCTG+AGG + chr8.3:31497925-31497944 MS.gene82987:CDS 45.0%
GGAGGACAAGAGAATGAATG+AGG + chr8.3:31497871-31497890 MS.gene82987:CDS 45.0%
GGGCCGCATTCAAAAATCAA+AGG - chr8.3:31498970-31498989 None:intergenic 45.0%
GTGGAAACCTTGCATTGGAA+AGG + chr8.3:31498437-31498456 MS.gene82987:CDS 45.0%
GTGTTTAGGTCTACACAGAC+AGG + chr8.3:31499057-31499076 MS.gene82987:CDS 45.0%
GTTATTGAGAATTGAGGGGC+AGG + chr8.3:31498618-31498637 MS.gene82987:CDS 45.0%
TAATCTACACTGGCCATGGA+GGG + chr8.3:31498368-31498387 MS.gene82987:CDS 45.0%
TAGGTCCTTGGACCTTATTC+AGG + chr8.3:31499089-31499108 MS.gene82987:CDS 45.0%
TAGGTCTACACAGACAGGTT+GGG + chr8.3:31499062-31499081 MS.gene82987:CDS 45.0%
TCGAAACCGTGTTGCACAAA+AGG + chr8.3:31499014-31499033 MS.gene82987:CDS 45.0%
TGATGTTCCGATGGATATGG+TGG - chr8.3:31497387-31497406 None:intergenic 45.0%
TGCACTATGGGATTGAGGTT+AGG + chr8.3:31498464-31498483 MS.gene82987:CDS 45.0%
TGCAGAGTTCTCTCATAGGA+GGG - chr8.3:31499446-31499465 None:intergenic 45.0%
TGTAAGGAGGCTTGTACTGA+CGG + chr8.3:31498874-31498893 MS.gene82987:CDS 45.0%
TGTGATCAGAAATTGGAGGG+TGG + chr8.3:31498418-31498437 MS.gene82987:CDS 45.0%
TTAGGTCTACACAGACAGGT+TGG + chr8.3:31499061-31499080 MS.gene82987:CDS 45.0%
TTCGCGGGATGAAGTATGAA+GGG + chr8.3:31498491-31498510 MS.gene82987:CDS 45.0%
TTGAGGTTAGGGTTATTCGC+GGG + chr8.3:31498476-31498495 MS.gene82987:CDS 45.0%
! ATATGGTGGTGATTTGGGGT+GGG - chr8.3:31497373-31497392 None:intergenic 45.0%
! CGACATTAATGAAGGAGCGT+GGG + chr8.3:31498116-31498135 MS.gene82987:CDS 45.0%
! CGATGGATATGGTGGTGATT+TGG - chr8.3:31497379-31497398 None:intergenic 45.0%
! CGTTTGAAGTGATGTTCCGA+TGG - chr8.3:31497396-31497415 None:intergenic 45.0%
! GGGGTGGATTCTGTAAATGA+TGG + chr8.3:31497656-31497675 MS.gene82987:CDS 45.0%
! TCTCAACCTTAATTTGGGGG+TGG + chr8.3:31497640-31497659 MS.gene82987:CDS 45.0%
!! GGAGGTATTGACGGATAAGA+TGG - chr8.3:31499273-31499292 None:intergenic 45.0%
!! GGTTGTTGAAGTTGATAGGG+AGG + chr8.3:31497904-31497923 MS.gene82987:CDS 45.0%
!!! AAGGGTAGAGTTTTGAGGTG+TGG - chr8.3:31497541-31497560 None:intergenic 45.0%
AACGCCACTCATTCCAAACC+TGG + chr8.3:31497412-31497431 MS.gene82987:CDS 50.0%
AATTCTTCTCTCGCCCTCCA+TGG - chr8.3:31498384-31498403 None:intergenic 50.0%
AGGAGGGAATTGTGTGTGAC+TGG + chr8.3:31498211-31498230 MS.gene82987:CDS 50.0%
AGGATAGTTGGACCGATACC+TGG + chr8.3:31498160-31498179 MS.gene82987:CDS 50.0%
AGGTCTACACAGACAGGTTG+GGG + chr8.3:31499063-31499082 MS.gene82987:CDS 50.0%
CTAAGATCGGGGGTGATAGT+AGG - chr8.3:31497299-31497318 None:intergenic 50.0%
GAGAGTGGGTGAGGAGAATA+GGG + chr8.3:31498009-31498028 MS.gene82987:CDS 50.0%
GAGGGCTGCGACATTAATGA+AGG + chr8.3:31498108-31498127 MS.gene82987:CDS 50.0%
GCGACATTAATGAAGGAGCG+TGG + chr8.3:31498115-31498134 MS.gene82987:CDS 50.0%
GGATGCAGAAGTTGCAGTTG+TGG + chr8.3:31497976-31497995 MS.gene82987:CDS 50.0%
GGCGAGAGAAGAATTCCTCA+AGG + chr8.3:31498389-31498408 MS.gene82987:CDS 50.0%
GGTGAGAGCGGTGTGATTTA+AGG - chr8.3:31497351-31497370 None:intergenic 50.0%
GGTTATGGAGAAGGAAAGCC+AGG + chr8.3:31497739-31497758 MS.gene82987:CDS 50.0%
GTGGAGGGGGAGACAATTAA+TGG + chr8.3:31498046-31498065 MS.gene82987:CDS 50.0%
TCCAGCCTGAATAAGGTCCA+AGG - chr8.3:31499097-31499116 None:intergenic 50.0%
TCCTTGGACCTTATTCAGGC+TGG + chr8.3:31499093-31499112 MS.gene82987:CDS 50.0%
TCGGGGGTGATAGTAGGAAT+AGG - chr8.3:31497293-31497312 None:intergenic 50.0%
TGAGAGTGGGTGAGGAGAAT+AGG + chr8.3:31498008-31498027 MS.gene82987:CDS 50.0%
TGCAGAAGTTGCAGTTGTGG+AGG + chr8.3:31497979-31497998 MS.gene82987:CDS 50.0%
TTCCGGATACTACTTTCCCG+TGG + chr8.3:31498812-31498831 MS.gene82987:CDS 50.0%
TTCGCTTCGCGTGAGAATTG+AGG + chr8.3:31497850-31497869 MS.gene82987:CDS 50.0%
! GAAGGGAGTGCATCTACTTC+TGG + chr8.3:31498508-31498527 MS.gene82987:CDS 50.0%
! GATATGGTGGTGATTTGGGG+TGG - chr8.3:31497374-31497393 None:intergenic 50.0%
! GGTTCCAGGTTTGGAATGAG+TGG - chr8.3:31497419-31497438 None:intergenic 50.0%
! TAATGAAGGAGCGTGGGCTA+TGG + chr8.3:31498122-31498141 MS.gene82987:CDS 50.0%
! TATGGTGGTGATTTGGGGTG+GGG - chr8.3:31497372-31497391 None:intergenic 50.0%
! TGAGCCCCTTTTGTGCAACA+CGG - chr8.3:31499023-31499042 None:intergenic 50.0%
!! CCAGGTGATGGAACTTGTGA+GGG + chr8.3:31497757-31497776 MS.gene82987:CDS 50.0%
AATTGAGGGGCAGGCGAAGA+TGG + chr8.3:31498627-31498646 MS.gene82987:CDS 55.0%
AATTGTCTCCCCCTCCACGT+TGG - chr8.3:31498044-31498063 None:intergenic 55.0%
AGGTTGGGGAGTTAGGTCCT+TGG + chr8.3:31499077-31499096 MS.gene82987:CDS 55.0%
ATTATGGCATCCCGGACGAG+TGG + chr8.3:31499469-31499488 MS.gene82987:CDS 55.0%
ATTGAGGGGCAGGCGAAGAT+GGG + chr8.3:31498628-31498647 MS.gene82987:CDS 55.0%
CATCAGAGTGGGGAACCTAG+TGG + chr8.3:31498844-31498863 MS.gene82987:CDS 55.0%
CCCTCACAAGTTCCATCACC+TGG - chr8.3:31497760-31497779 None:intergenic 55.0%
GCTTCGCGTGAGAATTGAGG+AGG + chr8.3:31497853-31497872 MS.gene82987:CDS 55.0%
GGAGAAGGAAAGCCAGGTGA+TGG + chr8.3:31497745-31497764 MS.gene82987:CDS 55.0%
GGAGGGTGGAAACCTTGCAT+TGG + chr8.3:31498432-31498451 MS.gene82987:CDS 55.0%
GGGTGAGGAGAATAGGGTGT+CGG + chr8.3:31498015-31498034 MS.gene82987:CDS 55.0%
GTGGAGGCAGAGATGAGAGT+GGG + chr8.3:31497995-31498014 MS.gene82987:CDS 55.0%
TGTGGAGGCAGAGATGAGAG+TGG + chr8.3:31497994-31498013 MS.gene82987:CDS 55.0%
TTATGGCATCCCGGACGAGT+GGG + chr8.3:31499470-31499489 MS.gene82987:CDS 55.0%
! CACAGACAGGTTGGGGAGTT+AGG + chr8.3:31499070-31499089 MS.gene82987:CDS 55.0%
!! GCCAGGTGATGGAACTTGTG+AGG + chr8.3:31497756-31497775 MS.gene82987:CDS 55.0%
ATCTTGCTCGCCCACTCGTC+CGG - chr8.3:31499483-31499502 None:intergenic 60.0%
GGCAGAGATGAGAGTGGGTG+AGG + chr8.3:31498000-31498019 MS.gene82987:CDS 60.0%
! GATTTGGGGTGGGGTGAGAG+CGG - chr8.3:31497363-31497382 None:intergenic 60.0%
! TGTCGGCTACCAACGTGGAG+GGG + chr8.3:31498032-31498051 MS.gene82987:CDS 60.0%
!! GTGTCGGCTACCAACGTGGA+GGG + chr8.3:31498031-31498050 MS.gene82987:CDS 60.0%
!! TAGGGTGTCGGCTACCAACG+TGG + chr8.3:31498027-31498046 MS.gene82987:CDS 60.0%
GTCGGCTACCAACGTGGAGG+GGG + chr8.3:31498033-31498052 MS.gene82987:CDS 65.0%
TCTTGCTCGCCCACTCGTCC+GGG - chr8.3:31499482-31499501 None:intergenic 65.0%
!! GGTGTCGGCTACCAACGTGG+AGG + chr8.3:31498030-31498049 MS.gene82987:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 31497221 31499518 31497221 ID=MS.gene82987
chr8.3 mRNA 31497221 31499518 31497221 ID=MS.gene82987.t1;Parent=MS.gene82987
chr8.3 exon 31497221 31499518 31497221 ID=MS.gene82987.t1.exon1;Parent=MS.gene82987.t1
chr8.3 CDS 31497221 31499518 31497221 ID=cds.MS.gene82987.t1;Parent=MS.gene82987.t1
Gene Sequence

>MS.gene82987

ATGGATCCTTTCCCATTAACCCTTGAAACCCATTCTTCTACTTCTATTTCTCAACCTTCTTCTCATGTTCCTATTCCTACTATCACCCCCGATCTTAGTATTTCTCATCAAATCCCTAGAATTTCAACCTTAAATCACACCGCTCTCACCCCACCCCAAATCACCACCATATCCATCGGAACATCACTTCAAACGCCACTCATTCCAAACCTGGAACCTTTTAACAATTTCTTCGCGTCCCAAGATTCCCAACAACAACCTAATTTCTCTCCCGAATCTGAAACCGATGATCAACCCAACCTATACGCCAAATTCTACCACACCTCAAAACTCTACCCTTTTCTCGAAATCGCTGATTCCAACAATGATTTTGTTGTTTCATTGGAGAATACGGTAAATGTTTGCAACCCGGACCTAAATCTCAACCTTAATTTGGGGGTGGATTCTGTAAATGATGGAAATGATCGAGCTATTGTTGTGGCTCCTGAGAAACAAGAGTCGAGTTTGATGGCATATATGGTTATGGAGAAGGAAAGCCAGGTGATGGAACTTGTGAGGGTTTCAGATCTGAGTATGAAGGATCAGATGCACTTTCGTCATAAGATAAGGAAGAGGCGAATGATTTATGATTCGCTTCGCGTGAGAATTGAGGAGGACAAGAGAATGAATGAGGAGAAGAAATTGGTTGTTGAAGTTGATAGGGAGGAAGAGAAGAGATTGTCTGAGGAAAGGAAATTGGATGAGGAGAAGATATTGGATGCAGAAGTTGCAGTTGTGGAGGCAGAGATGAGAGTGGGTGAGGAGAATAGGGTGTCGGCTACCAACGTGGAGGGGGAGACAATTAATGGAATAAGGCGTCGTAGTATCAACCACATTCATGGTTATATGAGGGCTGCGACATTAATGAAGGAGCGTGGGCTATGGTTGTATCGTGATCAAAGGATAGTTGGACCGATACCTGGAATTTATGTTGGTGATGTGTTTTTGTTTAGGAGGGAATTGTGTGTGACTGGTTTGCATATGCATATGCAAGCTGGTATTGATTATCTATCAAAGAGTATGTCTTATAATGGTGAACCGATTGCTACTAGTGTAATTGTTTCAGGTGGTTATGAGGATGACATGGAATTAGACGATGGTGATATTATAATCTACACTGGCCATGGAGGGCGAGAGAAGAATTCCTCAAGGCAAATTTGTGATCAGAAATTGGAGGGTGGAAACCTTGCATTGGAAAGGAGTATGCACTATGGGATTGAGGTTAGGGTTATTCGCGGGATGAAGTATGAAGGGAGTGCATCTACTTCTGGTAAGGTTTATGTTTACGATGGATTGTATAAGATTGTTAGTTGCTTGTTTGATATAGGAAAGTCTGGTTTTGGAGTTTATAAGTATAAGTTATTGAGAATTGAGGGGCAGGCGAAGATGGGTAGCACTGTTCTCAAAGATGCTAGGGATATTAAGAAAATTGGGTTGGATTTCATGCCAATGAGTTGTATCTCTATTGATATTTCAAATAATAGGGAAAATGTTCCAGTTAGGCTATTTAATGATATAGATGTCAATCATGAACCTCTTTATTATGAGTATCTTCCGGATACTACTTTCCCGTGGTTTGTGTTTCATCAGAGTGGGGAACCTAGTGGTTGTGAATGTAAGGAGGCTTGTACTGACGGTTGCTTTTGCGCTATGAAGAATGGAGGAGAGTTTCCCTATAATCCACATGGATTGCTTTTGAAAGGGAAACCTTTGATTTTTGAATGCGGCCCTTTTTGCTCATGTCCTCCTAATTGTCGAAACCGTGTTGCACAAAAGGGGCTCAAATATAGCTTGGAAGTGTTTAGGTCTACACAGACAGGTTGGGGAGTTAGGTCCTTGGACCTTATTCAGGCTGGATCCTTTATCTGCGAGTATACTGGTGTTGCTATAACTAGGGAACAAGCTCAAATTTTAACAATGAATGGGGTCTCGTTGGTGTATCCTAGTACATTTCCAAATAGATGGGTAGAATGGGGTGATTTATCTCAGATATACACTGAATATGTGCGACCATCTTATCCGTCAATACCTCCTTTAGATTATTTTCTGGATGTGTCAACAATGAGAAATGTTGCATCCTATATGAGCCATAGTTCGAGTCCAAATATATTCATTCAGTATGTTCTTTATGATCACAACAATTTGTTGTTCCCTCATGTCATGTTATATGCAATGGAGAACATCCCTCCTATGAGAGAACTCTGCATAGATTATGGCATCCCGGACGAGTGGGCGAGCAAGATGTCTATATGTATGTAA
Protein sequence

>MS.gene82987.t1

MDPFPLTLETHSSTSISQPSSHVPIPTITPDLSISHQIPRISTLNHTALTPPQITTISIGTSLQTPLIPNLEPFNNFFASQDSQQQPNFSPESETDDQPNLYAKFYHTSKLYPFLEIADSNNDFVVSLENTVNVCNPDLNLNLNLGVDSVNDGNDRAIVVAPEKQESSLMAYMVMEKESQVMELVRVSDLSMKDQMHFRHKIRKRRMIYDSLRVRIEEDKRMNEEKKLVVEVDREEEKRLSEERKLDEEKILDAEVAVVEAEMRVGEENRVSATNVEGETINGIRRRSINHIHGYMRAATLMKERGLWLYRDQRIVGPIPGIYVGDVFLFRRELCVTGLHMHMQAGIDYLSKSMSYNGEPIATSVIVSGGYEDDMELDDGDIIIYTGHGGREKNSSRQICDQKLEGGNLALERSMHYGIEVRVIRGMKYEGSASTSGKVYVYDGLYKIVSCLFDIGKSGFGVYKYKLLRIEGQAKMGSTVLKDARDIKKIGLDFMPMSCISIDISNNRENVPVRLFNDIDVNHEPLYYEYLPDTTFPWFVFHQSGEPSGCECKEACTDGCFCAMKNGGEFPYNPHGLLLKGKPLIFECGPFCSCPPNCRNRVAQKGLKYSLEVFRSTQTGWGVRSLDLIQAGSFICEYTGVAITREQAQILTMNGVSLVYPSTFPNRWVEWGDLSQIYTEYVRPSYPSIPPLDYFLDVSTMRNVASYMSHSSSPNIFIQYVLYDHNNLLFPHVMLYAMENIPPMRELCIDYGIPDEWASKMSICM