AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83177

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83177.t1 MTR_4g078170 100.000 216 0 0 1 216 1 216 6.72e-164 451
MS.gene83177.t1 MTR_5g030130 72.381 210 56 1 3 210 23 232 7.94e-115 327
MS.gene83177.t1 MTR_5g007230 73.516 219 45 4 1 213 1 212 2.35e-113 323
MS.gene83177.t1 MTR_8g066820 69.159 214 62 2 3 213 21 233 3.82e-107 308
MS.gene83177.t1 MTR_5g030130 77.465 142 32 0 1 142 21 162 1.97e-85 251
MS.gene83177.t1 MTR_7g102790 54.726 201 81 5 15 209 7 203 1.66e-70 214
MS.gene83177.t1 MTR_1g062160 53.465 202 81 4 15 209 7 202 4.01e-70 213
MS.gene83177.t1 MTR_2g060540 50.370 135 57 4 16 140 11 145 4.16e-40 134
MS.gene83177.t1 MTR_2g060500 48.889 135 59 3 16 140 11 145 1.17e-39 133
MS.gene83177.t1 MTR_4g116300 31.837 245 115 4 15 208 6 249 6.97e-38 132
MS.gene83177.t1 MTR_2g055210 32.922 243 115 5 12 208 99 339 8.85e-38 134
MS.gene83177.t1 MTR_2g084495 35.683 227 110 6 16 208 5 229 9.12e-38 131
MS.gene83177.t1 MTR_2g084525 48.936 141 62 4 16 146 5 145 4.49e-36 127
MS.gene83177.t1 MTR_2g084510 34.167 240 109 7 16 208 4 241 2.91e-35 125
MS.gene83177.t1 MTR_4g094680 36.486 222 112 6 12 208 19 236 5.21e-35 124
MS.gene83177.t1 MTR_2g036170 32.751 229 107 7 15 209 37 252 1.38e-30 113
MS.gene83177.t1 MTR_8g105930 33.951 162 82 6 1 141 1 158 1.89e-17 78.6
MS.gene83177.t1 MTR_8g097310 29.560 159 99 4 54 208 4 153 8.28e-17 74.7
MS.gene83177.t1 MTR_5g077740 27.000 200 104 8 15 208 23 186 3.83e-16 73.9
MS.gene83177.t1 MTR_1g032880 37.778 90 45 3 60 142 26 111 1.87e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83177.t1 AT4G17900 68.868 212 55 4 3 209 22 227 3.25e-101 292
MS.gene83177.t1 AT1G32700 67.907 215 62 4 6 216 2 213 9.27e-97 281
MS.gene83177.t1 AT4G17900 69.000 200 57 3 12 209 17 213 1.93e-96 280
MS.gene83177.t1 AT1G21000 51.046 239 76 5 9 208 7 243 1.68e-76 230
MS.gene83177.t1 AT1G21000 51.046 239 76 5 9 208 10 246 2.09e-76 230
MS.gene83177.t1 AT1G32700 67.797 177 51 3 43 216 1 174 1.32e-75 226
MS.gene83177.t1 AT1G76590 46.964 247 90 4 1 208 1 245 7.77e-72 219
MS.gene83177.t1 AT5G46710 52.404 208 87 5 9 208 22 225 7.88e-70 213
MS.gene83177.t1 AT5G46710 52.404 208 87 5 9 208 6 209 7.97e-70 212
MS.gene83177.t1 AT1G43000 51.442 208 87 3 15 208 16 223 4.56e-68 208
MS.gene83177.t1 AT1G43000 51.442 208 87 3 15 208 9 216 6.10e-68 207
MS.gene83177.t1 AT5G46710 61.940 134 50 1 9 141 22 155 1.73e-60 187
MS.gene83177.t1 AT2G27930 45.631 206 82 5 15 210 4 189 2.23e-51 164
MS.gene83177.t1 AT2G27930 45.631 206 82 5 15 210 4 189 2.23e-51 164
MS.gene83177.t1 AT2G27930 46.988 166 78 2 15 170 4 169 9.30e-47 152
MS.gene83177.t1 AT2G27930 46.988 166 78 2 15 170 4 169 9.30e-47 152
MS.gene83177.t1 AT2G12646 48.148 135 69 1 15 149 6 139 2.63e-39 136
MS.gene83177.t1 AT2G12646 48.148 135 69 1 15 149 6 139 2.63e-39 136
MS.gene83177.t1 AT2G12646 48.148 135 69 1 15 149 3 136 2.77e-39 135
MS.gene83177.t1 AT3G60670 46.970 132 69 1 10 141 2 132 4.83e-37 130
MS.gene83177.t1 AT1G31040 37.611 226 107 7 15 208 19 242 1.05e-36 129
MS.gene83177.t1 AT2G27930 46.053 152 53 4 68 210 61 192 4.88e-32 115
MS.gene83177.t1 AT2G27930 46.053 152 53 4 68 210 61 192 4.88e-32 115
MS.gene83177.t1 AT2G01818 35.071 211 116 8 17 208 13 221 2.04e-25 99.4
MS.gene83177.t1 AT2G01818 46.250 80 37 3 74 147 9 88 4.60e-13 65.1

Find 53 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGAGATACAGAAATTTC+TGG 0.238023 4.2:+31844130 MS.gene83177:CDS
TTTATTCTTCCTCAACTTCT+TGG 0.252653 4.2:-31844808 None:intergenic
TCCTTCAGCTTCTGCTCTCT+TGG 0.285072 4.2:+31844281 MS.gene83177:CDS
CTTCTGCTCTCTTGGTTGTA+AGG 0.335637 4.2:+31844289 MS.gene83177:CDS
CAAACAACATAGAACAATTC+AGG 0.377021 4.2:+31843715 MS.gene83177:CDS
TGGAATTAGGGAGGTGGAGG+AGG 0.387306 4.2:+31843527 MS.gene83177:intron
CTCAACCTAGGCCTGGAAAA+GGG 0.405265 4.2:+31844216 MS.gene83177:CDS
AAGAAACTTGTTTGAAGAAG+TGG 0.413060 4.2:-31843590 None:intergenic
CTTTGCACTGTTTATAACAT+AGG 0.413289 4.2:-31844171 None:intergenic
TTCATACCATGATGTGATAA+GGG 0.415618 4.2:+31844106 MS.gene83177:CDS
CTTCATACCATGATGTGATA+AGG 0.416229 4.2:+31844105 MS.gene83177:CDS
AGCAAGACAAACAGAACAAA+GGG 0.420807 4.2:-31843687 None:intergenic
CCTCAACCTAGGCCTGGAAA+AGG 0.425716 4.2:+31844215 MS.gene83177:CDS
GCTGTTGGAGGTGGTGTTGA+AGG 0.439304 4.2:-31844921 None:intergenic
ATTCTTCCTCAACTTCTTGG+AGG 0.449130 4.2:-31844805 None:intergenic
GAAAGGCCTCAACCTAGGCC+TGG 0.497293 4.2:+31844209 MS.gene83177:CDS
TAACTCACTGCTGTTGGAGG+TGG 0.507231 4.2:-31844930 None:intergenic
CTGTAACTCACTGCTGTTGG+AGG 0.521807 4.2:-31844933 None:intergenic
AAATGTTGAGTCAGAAAGAC+GGG 0.526940 4.2:+31844829 MS.gene83177:CDS
TCAATGAATGGCATTAGTAA+TGG 0.528017 4.2:+31844867 MS.gene83177:CDS
GAAGGCTACGGTCACAGACT+TGG 0.533320 4.2:-31844253 None:intergenic
AGAACAGTTAAGAGGAGGAA+AGG 0.533678 4.2:+31844954 MS.gene83177:CDS
ATCAATGTTGATAGATAAGG+AGG 0.535527 4.2:+31844081 MS.gene83177:intron
ATTCCACATAGAGCTCCAAT+GGG 0.539365 4.2:+31844978 MS.gene83177:CDS
TGAATGCAATATGTATTGCT+TGG 0.542264 4.2:+31843649 MS.gene83177:CDS
AATTCCACATAGAGCTCCAA+TGG 0.542578 4.2:+31844977 MS.gene83177:CDS
CAGAAACCCTTATCACATCA+TGG 0.550564 4.2:-31844112 None:intergenic
GCTACGGTCACAGACTTGGC+AGG 0.558501 4.2:-31844249 None:intergenic
AATGGCATTAGTAATGGAAG+TGG 0.564357 4.2:+31844873 MS.gene83177:CDS
TCAACCTAGGCCTGGAAAAG+GGG 0.568882 4.2:+31844217 MS.gene83177:CDS
TGAGTTACAGAACAGTTAAG+AGG 0.568904 4.2:+31844946 MS.gene83177:CDS
TGAATGAAAGGCCTCAACCT+AGG 0.570401 4.2:+31844204 MS.gene83177:CDS
TTGGAACCTCCAAGAAGTTG+AGG 0.571156 4.2:+31844799 MS.gene83177:CDS
AGTCAGAAAGACGGGTCTGA+TGG 0.574132 4.2:+31844837 MS.gene83177:CDS
GTCACAGACTTGGCAGGTGT+TGG 0.575139 4.2:-31844243 None:intergenic
TGAAGAAGTGGTTGCAACCA+TGG 0.585315 4.2:-31843578 None:intergenic
AGAAGCTGAAGGAATCAAGA+AGG 0.586801 4.2:-31844271 None:intergenic
GTTCTGTAACTCACTGCTGT+TGG 0.597323 4.2:-31844936 None:intergenic
AAGAAAACAAGTGGCCACCA+TGG 0.602626 4.2:+31843561 MS.gene83177:CDS
TGAAGGAATCAAGAAGGCTA+CGG 0.603890 4.2:-31844265 None:intergenic
AAGCAAGACAAACAGAACAA+AGG 0.605554 4.2:-31843688 None:intergenic
TATTGCTTGGATTGTAACAA+TGG 0.606739 4.2:+31843662 MS.gene83177:CDS
AAAATGTTGAGTCAGAAAGA+CGG 0.611575 4.2:+31844828 MS.gene83177:CDS
GATGGTGAAGAGTCAATGAA+TGG 0.621483 4.2:+31844855 MS.gene83177:CDS
GAAATTTCTGGACATAGCAG+AGG 0.630359 4.2:+31844142 MS.gene83177:CDS
ACCAAGAGAGCAGAAGCTGA+AGG 0.631097 4.2:-31844282 None:intergenic
CACTCTTGTGTGAATCTGAG+TGG 0.634730 4.2:-31843628 None:intergenic
ACAATGAAGAAGAAAACAAG+TGG 0.648499 4.2:+31843552 MS.gene83177:CDS
TCCACATAGAGCTCCAATGG+GGG 0.650434 4.2:+31844980 MS.gene83177:CDS
GTTACAGAACAGTTAAGAGG+AGG 0.658849 4.2:+31844949 MS.gene83177:CDS
CCACATAGAGCTCCAATGGG+GGG 0.658994 4.2:+31844981 MS.gene83177:CDS
TTCCACATAGAGCTCCAATG+GGG 0.732872 4.2:+31844979 MS.gene83177:CDS
AGAAGTGGTTGCAACCATGG+TGG 0.773987 4.2:-31843575 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATATAATAATCAAATTT+AGG - chr4.2:31844580-31844599 None:intergenic 10.0%
!!! AAAAATCAAATTTTTTCAAT+TGG - chr4.2:31843498-31843517 None:intergenic 10.0%
!!! AAAATCAAATTTTTTCAATT+GGG - chr4.2:31843497-31843516 None:intergenic 10.0%
!!! TTTTTTTTTTTTTGGAATTA+GGG + chr4.2:31843515-31843534 MS.gene83177:intron 10.0%
!!! TTTTTTTTTTTTTTGGAATT+AGG + chr4.2:31843514-31843533 MS.gene83177:intron 10.0%
!! ATTACAAAAAAAACATCAAA+AGG - chr4.2:31843455-31843474 None:intergenic 15.0%
!! TTACAAAAAAAACATCAAAA+GGG - chr4.2:31843454-31843473 None:intergenic 15.0%
!!! TTTTGCTAAAATAAATAATG+TGG + chr4.2:31844026-31844045 MS.gene83177:intron 15.0%
!! ATAACAAGCAATTATTCAAA+TGG - chr4.2:31843921-31843940 None:intergenic 20.0%
!! TAATTATTGAATTGGTCAAA+GGG - chr4.2:31843887-31843906 None:intergenic 20.0%
!! TATAAAGTATGCAAACATTA+AGG + chr4.2:31844673-31844692 MS.gene83177:intron 20.0%
!! TTAATTATTGAATTGGTCAA+AGG - chr4.2:31843888-31843907 None:intergenic 20.0%
!!! AAAGTGTTAAAATGGTTAAA+TGG + chr4.2:31844495-31844514 MS.gene83177:intron 20.0%
!!! ACTGATTTGAAACAAAAAAT+TGG - chr4.2:31843743-31843762 None:intergenic 20.0%
!!! ATTTTTTCTTGTTAGATTGT+TGG + chr4.2:31844780-31844799 MS.gene83177:intron 20.0%
!!! CATTTTTCTTACTTTTTCAA+AGG - chr4.2:31844715-31844734 None:intergenic 20.0%
!!! TATCAAATTTTCACAGAATT+GGG - chr4.2:31843803-31843822 None:intergenic 20.0%
!!! TCTATTGTAAAGTGTTAAAA+TGG + chr4.2:31844487-31844506 MS.gene83177:intron 20.0%
!!! TTATCAAATTTTCACAGAAT+TGG - chr4.2:31843804-31843823 None:intergenic 20.0%
!!! TTTATCAATGTTGATAGATA+AGG + chr4.2:31844078-31844097 MS.gene83177:intron 20.0%
! ACTTTATACAGAAAAGAAGT+AGG - chr4.2:31844661-31844680 None:intergenic 25.0%
! CAGTTAGATGTATATGAAAT+TGG - chr4.2:31844342-31844361 None:intergenic 25.0%
! CGTTAAGTCAAAATAATCAA+GGG - chr4.2:31844373-31844392 None:intergenic 25.0%
! TCAAATCAGTTAATTGTGTT+TGG + chr4.2:31843753-31843772 MS.gene83177:intron 25.0%
!!! AGATTGTGTTTTTGAATGAA+AGG + chr4.2:31844192-31844211 MS.gene83177:CDS 25.0%
!!! ATTATTTTGACTTAACGCTA+TGG + chr4.2:31844376-31844395 MS.gene83177:intron 25.0%
!!! TTTTTTTTTTGGAATTAGGG+AGG + chr4.2:31843518-31843537 MS.gene83177:intron 25.0%
AAAATGTTGAGTCAGAAAGA+CGG + chr4.2:31844828-31844847 MS.gene83177:CDS 30.0%
ACAATGAAGAAGAAAACAAG+TGG + chr4.2:31843552-31843571 MS.gene83177:CDS 30.0%
CAAACAACATAGAACAATTC+AGG + chr4.2:31843715-31843734 MS.gene83177:CDS 30.0%
CTTTGCACTGTTTATAACAT+AGG - chr4.2:31844174-31844193 None:intergenic 30.0%
GCGTTAAGTCAAAATAATCA+AGG - chr4.2:31844374-31844393 None:intergenic 30.0%
TATTGCTTGGATTGTAACAA+TGG + chr4.2:31843662-31843681 MS.gene83177:CDS 30.0%
TCAATGAATGGCATTAGTAA+TGG + chr4.2:31844867-31844886 MS.gene83177:CDS 30.0%
TCAGTTAATTGTGTTTGGTT+TGG + chr4.2:31843758-31843777 MS.gene83177:intron 30.0%
TGAATGCAATATGTATTGCT+TGG + chr4.2:31843649-31843668 MS.gene83177:CDS 30.0%
TTATACAGAAAAGAAGTAGG+AGG - chr4.2:31844658-31844677 None:intergenic 30.0%
TTCATACCATGATGTGATAA+GGG + chr4.2:31844106-31844125 MS.gene83177:CDS 30.0%
TTTATTCTTCCTCAACTTCT+TGG - chr4.2:31844811-31844830 None:intergenic 30.0%
! AAGAAACTTGTTTGAAGAAG+TGG - chr4.2:31843593-31843612 None:intergenic 30.0%
! AGAAAAATGATGATGAGAGT+AGG + chr4.2:31844726-31844745 MS.gene83177:intron 30.0%
! ATCAATGTTGATAGATAAGG+AGG + chr4.2:31844081-31844100 MS.gene83177:intron 30.0%
!! TTCTGAGATACAGAAATTTC+TGG + chr4.2:31844130-31844149 MS.gene83177:CDS 30.0%
AAATGTTGAGTCAGAAAGAC+GGG + chr4.2:31844829-31844848 MS.gene83177:CDS 35.0%
AAGCAAGACAAACAGAACAA+AGG - chr4.2:31843691-31843710 None:intergenic 35.0%
AGCAAGACAAACAGAACAAA+GGG - chr4.2:31843690-31843709 None:intergenic 35.0%
CAATAGAAAACTTTCCACAC+AGG - chr4.2:31844474-31844493 None:intergenic 35.0%
CTTCATACCATGATGTGATA+AGG + chr4.2:31844105-31844124 MS.gene83177:CDS 35.0%
GTGATCCAAAAATGCAGTTT+GGG - chr4.2:31844525-31844544 None:intergenic 35.0%
TGAGTTACAGAACAGTTAAG+AGG + chr4.2:31844946-31844965 MS.gene83177:CDS 35.0%
TGATCCAAAAATGCAGTTTG+GGG - chr4.2:31844524-31844543 None:intergenic 35.0%
TGTGATCCAAAAATGCAGTT+TGG - chr4.2:31844526-31844545 None:intergenic 35.0%
! AAAATGATGATGAGAGTAGG+TGG + chr4.2:31844729-31844748 MS.gene83177:intron 35.0%
! AATGGCATTAGTAATGGAAG+TGG + chr4.2:31844873-31844892 MS.gene83177:CDS 35.0%
! TGTTGATTGTTCTGCTTTGA+TGG + chr4.2:31843964-31843983 MS.gene83177:intron 35.0%
!!! TTTTGACTTAACGCTATGGT+TGG + chr4.2:31844380-31844399 MS.gene83177:intron 35.0%
!!! TTTTTTTGGAATTAGGGAGG+TGG + chr4.2:31843521-31843540 MS.gene83177:intron 35.0%
AATTCCACATAGAGCTCCAA+TGG + chr4.2:31844977-31844996 MS.gene83177:CDS 40.0%
AGAACAGTTAAGAGGAGGAA+AGG + chr4.2:31844954-31844973 MS.gene83177:CDS 40.0%
AGAAGCTGAAGGAATCAAGA+AGG - chr4.2:31844274-31844293 None:intergenic 40.0%
ATTCCACATAGAGCTCCAAT+GGG + chr4.2:31844978-31844997 MS.gene83177:CDS 40.0%
ATTCTTCCTCAACTTCTTGG+AGG - chr4.2:31844808-31844827 None:intergenic 40.0%
CAGAAACCCTTATCACATCA+TGG - chr4.2:31844115-31844134 None:intergenic 40.0%
GATCCAAAAATGCAGTTTGG+GGG - chr4.2:31844523-31844542 None:intergenic 40.0%
GATGGTGAAGAGTCAATGAA+TGG + chr4.2:31844855-31844874 MS.gene83177:CDS 40.0%
GATTGTTCTGCTTTGATGGA+TGG + chr4.2:31843968-31843987 MS.gene83177:intron 40.0%
GTTACAGAACAGTTAAGAGG+AGG + chr4.2:31844949-31844968 MS.gene83177:CDS 40.0%
TCAGTCGGGTAAGTAAAGTT+TGG + chr4.2:31844414-31844433 MS.gene83177:intron 40.0%
TGAAGGAATCAAGAAGGCTA+CGG - chr4.2:31844268-31844287 None:intergenic 40.0%
! GAAATTTCTGGACATAGCAG+AGG + chr4.2:31844142-31844161 MS.gene83177:CDS 40.0%
! TTGGTAATCTGGAGTTCAGT+CGG + chr4.2:31844399-31844418 MS.gene83177:intron 40.0%
!! TAACGCTATGGTTGGTAATC+TGG + chr4.2:31844388-31844407 MS.gene83177:intron 40.0%
AAGAAAACAAGTGGCCACCA+TGG + chr4.2:31843561-31843580 MS.gene83177:CDS 45.0%
ATAATGATTCCTCCCCCCAT+TGG - chr4.2:31844996-31845015 None:intergenic 45.0%
CACTCTTGTGTGAATCTGAG+TGG - chr4.2:31843631-31843650 None:intergenic 45.0%
CTTCTGCTCTCTTGGTTGTA+AGG + chr4.2:31844289-31844308 MS.gene83177:CDS 45.0%
GTTCTGTAACTCACTGCTGT+TGG - chr4.2:31844939-31844958 None:intergenic 45.0%
TAGAAGCTGATTGACCTGTG+TGG + chr4.2:31844457-31844476 MS.gene83177:intron 45.0%
TGAAGAAGTGGTTGCAACCA+TGG - chr4.2:31843581-31843600 None:intergenic 45.0%
TGAATGAAAGGCCTCAACCT+AGG + chr4.2:31844204-31844223 MS.gene83177:CDS 45.0%
TTCCACATAGAGCTCCAATG+GGG + chr4.2:31844979-31844998 MS.gene83177:CDS 45.0%
TTGGAACCTCCAAGAAGTTG+AGG + chr4.2:31844799-31844818 MS.gene83177:CDS 45.0%
! GATCCCCCAAACTGCATTTT+TGG + chr4.2:31844517-31844536 MS.gene83177:intron 45.0%
! GTTCTGCTTTGATGGATGGA+TGG + chr4.2:31843972-31843991 MS.gene83177:intron 45.0%
! TGGTAATCTGGAGTTCAGTC+GGG + chr4.2:31844400-31844419 MS.gene83177:intron 45.0%
!!! TTTTGGAATTAGGGAGGTGG+AGG + chr4.2:31843524-31843543 MS.gene83177:intron 45.0%
!!! ATTTGATAATAAAATAATTT+TGG + chr4.2:31843815-31843834 MS.gene83177:intron 5.0%
!!! ATTTGATTTTTTTTTTTTTT+TGG + chr4.2:31843507-31843526 MS.gene83177:intron 5.0%
!!! ATTTTTTTTAATTATTGAAT+TGG - chr4.2:31843895-31843914 None:intergenic 5.0%
!!! GATAATTTTTATTTAAATTA+TGG + chr4.2:31843994-31844013 MS.gene83177:intron 5.0%
ACCAAGAGAGCAGAAGCTGA+AGG - chr4.2:31844285-31844304 None:intergenic 50.0%
AGAAGTGGTTGCAACCATGG+TGG - chr4.2:31843578-31843597 None:intergenic 50.0%
AGTCAGAAAGACGGGTCTGA+TGG + chr4.2:31844837-31844856 MS.gene83177:CDS 50.0%
CTCAACCTAGGCCTGGAAAA+GGG + chr4.2:31844216-31844235 MS.gene83177:CDS 50.0%
CTGTAACTCACTGCTGTTGG+AGG - chr4.2:31844936-31844955 None:intergenic 50.0%
TAACTCACTGCTGTTGGAGG+TGG - chr4.2:31844933-31844952 None:intergenic 50.0%
TCAACCTAGGCCTGGAAAAG+GGG + chr4.2:31844217-31844236 MS.gene83177:CDS 50.0%
TCCACATAGAGCTCCAATGG+GGG + chr4.2:31844980-31844999 MS.gene83177:CDS 50.0%
TCCTTCAGCTTCTGCTCTCT+TGG + chr4.2:31844281-31844300 MS.gene83177:CDS 50.0%
CCACATAGAGCTCCAATGGG+GGG + chr4.2:31844981-31845000 MS.gene83177:CDS 55.0%
CCTCAACCTAGGCCTGGAAA+AGG + chr4.2:31844215-31844234 MS.gene83177:CDS 55.0%
GAAGGCTACGGTCACAGACT+TGG - chr4.2:31844256-31844275 None:intergenic 55.0%
GTCACAGACTTGGCAGGTGT+TGG - chr4.2:31844246-31844265 None:intergenic 55.0%
TGGAATTAGGGAGGTGGAGG+AGG + chr4.2:31843527-31843546 MS.gene83177:intron 55.0%
! CCTTTTCCAGGCCTAGGTTG+AGG - chr4.2:31844218-31844237 None:intergenic 55.0%
! GCTGTTGGAGGTGGTGTTGA+AGG - chr4.2:31844924-31844943 None:intergenic 55.0%
!!! GTGTTGGTGACCCCTTTTCC+AGG - chr4.2:31844230-31844249 None:intergenic 55.0%
CATAGAGCTCCAATGGGGGG+AGG + chr4.2:31844984-31845003 MS.gene83177:CDS 60.0%
CCCCCCATTGGAGCTCTATG+TGG - chr4.2:31844984-31845003 None:intergenic 60.0%
GAAAGGCCTCAACCTAGGCC+TGG + chr4.2:31844209-31844228 MS.gene83177:CDS 60.0%
GCTACGGTCACAGACTTGGC+AGG - chr4.2:31844252-31844271 None:intergenic 60.0%
! GTGACCCCTTTTCCAGGCCT+AGG - chr4.2:31844224-31844243 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 31843428 31845025 31843428 ID=MS.gene83177
chr4.2 mRNA 31843428 31845025 31843428 ID=MS.gene83177.t1;Parent=MS.gene83177
chr4.2 exon 31843428 31843430 31843428 ID=MS.gene83177.t1.exon1;Parent=MS.gene83177.t1
chr4.2 CDS 31843428 31843430 31843428 ID=cds.MS.gene83177.t1;Parent=MS.gene83177.t1
chr4.2 exon 31843536 31843736 31843536 ID=MS.gene83177.t1.exon2;Parent=MS.gene83177.t1
chr4.2 CDS 31843536 31843736 31843536 ID=cds.MS.gene83177.t1;Parent=MS.gene83177.t1
chr4.2 exon 31844095 31844310 31844095 ID=MS.gene83177.t1.exon3;Parent=MS.gene83177.t1
chr4.2 CDS 31844095 31844310 31844095 ID=cds.MS.gene83177.t1;Parent=MS.gene83177.t1
chr4.2 exon 31844795 31845025 31844795 ID=MS.gene83177.t1.exon4;Parent=MS.gene83177.t1
chr4.2 CDS 31844795 31845025 31844795 ID=cds.MS.gene83177.t1;Parent=MS.gene83177.t1
Gene Sequence

>MS.gene83177

ATGGTTAGTGTCATTAACAAATACCCTTTTGATGTTTTTTTTGTAATTTTTTATTTTAATTATATACCCAATTGAAAAAATTTGATTTTTTTTTTTTTTTGGAATTAGGGAGGTGGAGGAGGTAACAATGAAGAAGAAAACAAGTGGCCACCATGGTTGCAACCACTTCTTCAAACAAGTTTCTTTGTTCAATGCAAAGTCCACTCAGATTCACACAAGAGTGAATGCAATATGTATTGCTTGGATTGTAACAATGGTGCCCTTTGTTCTGTTTGTCTTGCTTCTCACAAACAACATAGAACAATTCAGGTACCAATTTTTTGTTTCAAATCAGTTAATTGTGTTTGGTTTGGAATTCATGACTCAATTTGACCCAATTCTGTGAAAATTTGATAATAAAATAATTTTGGTGTTTGTAATTGATTGTGTACTGTGCATTGTGATGATCAAATAATTCCCTTTGACCAATTCAATAATTAAAAAAAATTTACCATTTGAATAATTGCTTGTTATTATTATTACTCAATTATGTTGTCTGTTGATTGTTCTGCTTTGATGGATGGATGGATAATTTTTATTTAAATTATGGTATTTTGTTTTTTGCTAAAATAAATAATGTGGTTGATATTCTCTGATGAAGATTTTTTTTTTTTATCAATGTTGATAGATAAGGAGGTCTTCATACCATGATGTGATAAGGGTTTCTGAGATACAGAAATTTCTGGACATAGCAGAGGTTCAAACCTATGTTATAAACAGTGCAAAGATTGTGTTTTTGAATGAAAGGCCTCAACCTAGGCCTGGAAAAGGGGTCACCAACACCTGCCAAGTCTGTGACCGTAGCCTTCTTGATTCCTTCAGCTTCTGCTCTCTTGGTTGTAAGGTATGTGATACATAGTTAAATTCATAATCCAATTTCATATACATCTAACTGTTTTTTATCCCTTGATTATTTTGACTTAACGCTATGGTTGGTAATCTGGAGTTCAGTCGGGTAAGTAAAGTTTGGTCAATGATTGTTTCAGCTTATAGAAGCTGATTGACCTGTGTGGAAAGTTTTCTATTGTAAAGTGTTAAAATGGTTAAATGGATCCCCCAAACTGCATTTTTGGATCACATTAGCAATAACAAAGTATAGCTTATTGTTTTCCTAAATTTGATTATTATATTGTTTTTATAGAAATGTTGCACTTGTATGTAATCTGTAAAGTAAAACAATTGTAACTACCTCCTACTTCTTTTCTGTATAAAGTATGCAAACATTAAGGTGTACTTTTTATGTTTCCTTTGAAAAAGTAAGAAAAATGATGATGAGAGTAGGTGGATTTATAAATGACTTCTTCAACTTTTTGATTTTTTCTTGTTAGATTGTTGGAACCTCCAAGAAGTTGAGGAAGAATAAAATGTTGAGTCAGAAAGACGGGTCTGATGGTGAAGAGTCAATGAATGGCATTAGTAATGGAAGTGGCAGAAACAGAAGTCATAGTTTTACACCTTCAACACCACCTCCAACAGCAGTGAGTTACAGAACAGTTAAGAGGAGGAAAGGAATTCCACATAGAGCTCCAATGGGGGGAGGAATCATTATAGAATTCTAA
Protein sequence

>MS.gene83177.t1

MGGGGGNNEEENKWPPWLQPLLQTSFFVQCKVHSDSHKSECNMYCLDCNNGALCSVCLASHKQHRTIQIRRSSYHDVIRVSEIQKFLDIAEVQTYVINSAKIVFLNERPQPRPGKGVTNTCQVCDRSLLDSFSFCSLGCKIVGTSKKLRKNKMLSQKDGSDGEESMNGISNGSGRNRSHSFTPSTPPPTAVSYRTVKRRKGIPHRAPMGGGIIIEF