AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83205

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83205.t1 MTR_7g057230 89.977 439 44 0 4 442 219 657 0.0 808
MS.gene83205.t1 MTR_6g047750 51.339 448 191 8 12 441 223 661 1.45e-135 404
MS.gene83205.t1 MTR_4g074320 83.511 188 31 0 24 211 1 188 6.52e-111 325
MS.gene83205.t1 MTR_4g074310 64.783 230 47 3 81 310 1 196 4.15e-92 278
MS.gene83205.t1 MTR_3g089055 39.426 383 216 7 69 441 188 564 4.70e-79 255
MS.gene83205.t1 MTR_3g089055 39.426 383 216 7 69 441 193 569 5.07e-79 256
MS.gene83205.t1 MTR_1g029420 39.733 375 210 6 77 441 223 591 7.20e-74 243
MS.gene83205.t1 MTR_4g133660 33.777 376 232 6 77 441 173 542 4.10e-59 202
MS.gene83205.t1 MTR_2g082090 38.926 298 170 5 150 441 287 578 3.61e-58 201
MS.gene83205.t1 MTR_5g097480 31.807 415 262 7 37 441 140 543 2.04e-57 198
MS.gene83205.t1 MTR_3g056110 34.018 341 211 6 108 441 208 541 4.93e-55 191
MS.gene83205.t1 MTR_5g094450 32.546 381 233 11 77 441 159 531 2.75e-52 184
MS.gene83205.t1 MTR_2g026250 31.672 341 221 6 107 441 261 595 5.38e-49 176
MS.gene83205.t1 MTR_7g027190 31.438 299 180 8 150 441 392 672 7.44e-33 131
MS.gene83205.t1 MTR_8g442410 29.204 339 215 7 119 441 195 524 3.82e-32 128
MS.gene83205.t1 MTR_3g065980 28.385 384 240 10 78 441 171 539 5.23e-32 128
MS.gene83205.t1 MTR_4g102790 32.453 265 162 8 187 441 404 661 2.31e-28 118
MS.gene83205.t1 MTR_4g104020 28.947 304 204 6 142 441 224 519 3.58e-28 117
MS.gene83205.t1 MTR_4g064160 25.330 379 254 8 80 441 317 683 2.57e-25 109
MS.gene83205.t1 MTR_4g064200 25.397 378 253 9 80 441 285 649 8.03e-24 104
MS.gene83205.t1 MTR_2g034250 29.310 290 191 6 161 441 300 584 2.75e-23 102
MS.gene83205.t1 MTR_7g109580 27.027 259 181 3 187 441 299 553 9.22e-23 101
MS.gene83205.t1 MTR_7g074650 26.230 366 245 10 84 441 443 791 1.87e-22 100
MS.gene83205.t1 MTR_2g034260 30.000 270 174 6 181 441 320 583 2.81e-22 100
MS.gene83205.t1 MTR_2g097350 25.201 373 260 8 80 441 275 639 3.06e-22 100
MS.gene83205.t1 MTR_2g097390 24.800 375 259 10 80 441 276 640 3.24e-22 99.8
MS.gene83205.t1 MTR_2g097463 27.206 272 186 6 179 441 386 654 6.09e-22 99.0
MS.gene83205.t1 MTR_2g097310 25.199 377 257 9 80 442 273 638 8.22e-22 98.6
MS.gene83205.t1 MTR_4g097080 26.389 288 192 6 164 441 225 502 1.39e-21 97.4
MS.gene83205.t1 MTR_2g097410 23.016 378 268 9 80 442 372 741 1.57e-21 97.8
MS.gene83205.t1 MTR_4g077760 34.440 241 139 9 160 394 276 503 4.71e-21 96.3
MS.gene83205.t1 MTR_2g097380 26.027 292 182 7 160 442 295 561 1.47e-20 94.7
MS.gene83205.t1 MTR_4g064150 22.590 363 260 8 95 442 377 733 1.86e-20 94.7
MS.gene83205.t1 MTR_2g097473 26.642 274 186 7 180 441 383 653 2.08e-20 94.4
MS.gene83205.t1 MTR_7g062120 23.545 378 262 9 80 441 373 739 2.54e-20 94.4
MS.gene83205.t1 MTR_4g064180 24.281 313 223 6 135 439 317 623 2.87e-20 94.0
MS.gene83205.t1 MTR_2g034280 28.425 292 192 8 160 441 290 574 3.85e-20 93.6
MS.gene83205.t1 MTR_2g097467 25.887 282 197 6 169 441 375 653 7.86e-20 92.8
MS.gene83205.t1 MTR_4g076140 26.554 354 189 10 150 441 124 468 1.54e-19 91.3
MS.gene83205.t1 MTR_1g096030 26.515 396 239 15 80 441 81 458 1.35e-18 88.2
MS.gene83205.t1 MTR_4g064120 22.364 313 229 6 135 439 317 623 1.50e-18 88.6
MS.gene83205.t1 MTR_5g015950 26.250 320 192 9 160 441 132 445 1.03e-17 85.5
MS.gene83205.t1 MTR_3g053270 26.250 320 192 9 160 441 132 445 1.03e-17 85.5
MS.gene83205.t1 MTR_3g022830 24.854 342 196 8 153 441 102 435 1.29e-17 85.1
MS.gene83205.t1 MTR_5g009080 33.113 151 94 3 296 441 329 477 2.75e-15 78.2
MS.gene83205.t1 MTR_1g086970 24.710 259 186 4 187 441 224 477 2.58e-14 75.1
MS.gene83205.t1 MTR_4g026485 24.934 381 250 12 77 441 263 623 2.82e-14 75.5
MS.gene83205.t1 MTR_5g015490 24.913 289 201 7 164 441 206 489 3.40e-13 71.6
MS.gene83205.t1 MTR_4g095500 23.810 231 146 3 241 441 237 467 2.29e-12 68.9
MS.gene83205.t1 MTR_2g089100 22.468 316 199 8 160 441 139 442 8.67e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83205.t1 AT5G52510 47.955 440 194 11 21 441 216 639 1.42e-117 357
MS.gene83205.t1 AT1G21450 34.598 448 255 12 11 441 166 592 7.13e-69 229
MS.gene83205.t1 AT5G48150 32.719 434 260 12 29 441 67 489 7.81e-61 206
MS.gene83205.t1 AT5G48150 32.719 434 260 12 29 441 67 489 7.81e-61 206
MS.gene83205.t1 AT5G48150 32.719 434 260 12 29 441 67 489 7.81e-61 206
MS.gene83205.t1 AT5G48150 32.719 434 260 12 29 441 67 489 7.81e-61 206
MS.gene83205.t1 AT2G04890 33.512 373 229 8 77 441 51 412 9.42e-58 196
MS.gene83205.t1 AT1G50600 34.203 345 205 7 108 441 192 525 7.25e-53 185
MS.gene83205.t1 AT1G50600 34.203 345 205 7 108 441 263 596 2.48e-52 185
MS.gene83205.t1 AT1G50600 34.203 345 205 7 108 441 267 600 2.67e-52 185
MS.gene83205.t1 AT4G17230 34.251 327 200 5 77 394 156 476 5.38e-52 183
MS.gene83205.t1 AT4G17230 34.251 327 200 5 77 394 156 476 5.38e-52 183
MS.gene83205.t1 AT3G03450 34.138 290 174 7 159 441 265 544 7.88e-35 136
MS.gene83205.t1 AT1G66350 30.275 327 204 10 121 441 197 505 2.16e-34 134
MS.gene83205.t1 AT5G17490 33.233 331 196 11 120 441 201 515 3.64e-34 134
MS.gene83205.t1 AT1G14920 30.514 331 206 11 120 441 213 528 8.00e-31 124
MS.gene83205.t1 AT2G01570 30.931 333 202 12 120 441 265 580 9.06e-31 125
MS.gene83205.t1 AT2G29065 25.397 378 265 8 80 442 253 628 4.33e-29 120
MS.gene83205.t1 AT3G50650 30.537 298 181 6 160 441 254 541 5.02e-27 114
MS.gene83205.t1 AT5G66770 32.787 244 144 7 160 394 300 532 4.77e-25 108
MS.gene83205.t1 AT5G59450 26.176 340 223 11 120 441 268 597 9.93e-25 107
MS.gene83205.t1 AT3G46600 26.207 290 197 6 161 441 89 370 2.43e-23 101
MS.gene83205.t1 AT2G29060 23.936 376 266 8 80 441 322 691 4.27e-23 102
MS.gene83205.t1 AT2G29060 23.873 377 267 8 80 442 285 655 4.39e-23 102
MS.gene83205.t1 AT3G46600 26.207 290 197 6 161 441 297 578 1.37e-22 100
MS.gene83205.t1 AT3G46600 26.207 290 197 6 161 441 265 546 1.69e-22 100
MS.gene83205.t1 AT1G50420 25.349 430 244 12 80 441 58 478 5.18e-22 98.6
MS.gene83205.t1 AT2G37650 23.747 379 258 8 80 441 348 712 5.60e-22 99.4
MS.gene83205.t1 AT2G37650 23.747 379 258 8 80 441 336 700 5.94e-22 99.4
MS.gene83205.t1 AT1G07530 22.951 366 274 4 84 442 401 765 6.00e-22 99.4
MS.gene83205.t1 AT3G54220 28.276 290 187 9 156 441 377 649 8.64e-21 95.5
MS.gene83205.t1 AT1G07520 23.607 377 264 9 84 442 324 694 1.14e-20 95.5
MS.gene83205.t1 AT1G07520 23.607 377 264 9 84 442 323 693 1.22e-20 95.1
MS.gene83205.t1 AT1G07520 23.607 377 264 9 84 442 159 529 1.38e-20 94.7
MS.gene83205.t1 AT1G07520 23.607 377 264 9 84 442 159 529 1.38e-20 94.7
MS.gene83205.t1 AT3G49950 24.311 399 259 10 73 442 24 408 1.29e-18 87.8
MS.gene83205.t1 AT4G37650 25.122 410 267 13 62 441 129 528 1.93e-18 88.2
MS.gene83205.t1 AT1G63100 25.000 316 211 9 106 412 309 607 3.34e-18 87.8
MS.gene83205.t1 AT1G63100 25.000 316 211 9 106 412 284 582 3.63e-18 87.4
MS.gene83205.t1 AT5G41920 27.891 294 182 12 156 441 128 399 7.52e-16 79.7
MS.gene83205.t1 AT1G55580 26.510 298 193 8 160 441 157 444 3.90e-14 74.3
MS.gene83205.t1 AT2G45160 25.369 339 215 13 120 441 321 638 5.74e-13 71.2

Find 72 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATTCGATAGTTGGTTAT+TGG 0.173769 7.3:+47560522 MS.gene83205:CDS
GAAATCAATCAGAGAACTTT+TGG 0.191309 7.3:+47560882 MS.gene83205:CDS
CTCTCAATGTTGCATCTTCT+TGG 0.201520 7.3:+47561493 MS.gene83205:CDS
TTGCTTCAAGCTCTCTTTCA+TGG 0.207322 7.3:+47560663 MS.gene83205:CDS
GAATGGTCACTAGTTGTTTC+TGG 0.207414 7.3:+47560238 MS.gene83205:CDS
CTCAATTTCTGAAGGCAAAA+TGG 0.207878 7.3:+47560447 MS.gene83205:CDS
TCCAAGCATTGTCACAGTTT+TGG 0.258937 7.3:+47561110 MS.gene83205:CDS
GAGAGATTAAAGCTTGAAAA+AGG 0.266629 7.3:+47561246 MS.gene83205:CDS
GAGTTAGTTCGATGAGATTT+AGG 0.271519 7.3:-47560954 None:intergenic
TGAGACTCGCATGGAGTTCT+TGG 0.274180 7.3:-47560798 None:intergenic
GGCTTCAAGGATTGCGATAT+TGG 0.275042 7.3:-47560689 None:intergenic
AAAGAAGGTGAGAATGAAAT+AGG 0.311106 7.3:-47560337 None:intergenic
TCACACTCTTTGTTGTATCT+TGG 0.314011 7.3:-47560717 None:intergenic
CGTGCACGCATGAGCATGGC+TGG 0.338695 7.3:+47561348 MS.gene83205:CDS
CAAGAAGATGATGTATGAAT+TGG 0.343347 7.3:+47560179 None:intergenic
GCATGAGCATGGCTGGGTTT+AGG 0.345416 7.3:+47561355 MS.gene83205:CDS
TTGCTGAACAATTCCGCAAC+TGG 0.346117 7.3:-47560598 None:intergenic
GGTCGAGAAGCTGAGAGTTT+AGG 0.349907 7.3:+47560904 MS.gene83205:CDS
TGGAGTTTGCTTTGGTTGGA+TGG 0.363970 7.3:+47561464 MS.gene83205:CDS
AGCATCCATTAACGATTGTT+TGG 0.379805 7.3:-47560419 None:intergenic
AATGGTCACTAGTTGTTTCT+GGG 0.387462 7.3:+47560239 MS.gene83205:CDS
GTTACTAACTCAGTAACTTT+CGG 0.401174 7.3:-47561393 None:intergenic
TCCGTTGACACGCTCTCATC+TGG 0.405421 7.3:-47561045 None:intergenic
ATGGTGGAGTTTGCTTTGGT+TGG 0.406060 7.3:+47561460 MS.gene83205:CDS
GGAGTTTGCTTTGGTTGGAT+GGG 0.411286 7.3:+47561465 MS.gene83205:CDS
GTCGTGATCTTCAACATTGC+TGG 0.414937 7.3:-47560829 None:intergenic
TATCTTGGAATGCGGCTTCA+AGG 0.418126 7.3:-47560702 None:intergenic
CAAAACTAAAATTCGATAGT+TGG 0.427464 7.3:+47560515 MS.gene83205:CDS
AACAATTCCGCAACTGGAGG+TGG 0.434762 7.3:-47560592 None:intergenic
GCACAGTAATTCTGCTACCT+TGG 0.435103 7.3:-47561428 None:intergenic
TGTTTCTGACCATTCGTTGT+TGG 0.451392 7.3:-47560272 None:intergenic
AAGCTGAGAGTTTAGGAATC+GGG 0.451634 7.3:+47560911 MS.gene83205:CDS
GAGAATGGTGGAGTTTGCTT+TGG 0.469489 7.3:+47561456 MS.gene83205:CDS
GAACTCCATGCGAGTCTCAA+TGG 0.484923 7.3:+47560802 MS.gene83205:CDS
TCCAAAACTGTGACAATGCT+TGG 0.490294 7.3:-47561111 None:intergenic
AATCGGGTTTGAATTCAAAC+TGG 0.492177 7.3:+47560927 MS.gene83205:CDS
TCAGCCACACGTGAAACTAA+TGG 0.502475 7.3:-47561159 None:intergenic
GAAGCTGAGAGTTTAGGAAT+CGG 0.518502 7.3:+47560910 MS.gene83205:CDS
CTTTGTTGTATCTTGGAATG+CGG 0.536014 7.3:-47560710 None:intergenic
GCTGGTGAAACAATAGATGA+AGG 0.542748 7.3:-47560385 None:intergenic
TGTGAAGAATCTTTGTGTTG+TGG 0.543930 7.3:+47560735 MS.gene83205:CDS
TGTTTGGAAAACTCAGAAGC+TGG 0.544411 7.3:-47560403 None:intergenic
AGAATCTATTGAATTTGCGA+TGG 0.545507 7.3:+47561209 MS.gene83205:CDS
TGCAGTTGCTTGTGAAGGGA+GGG 0.563266 7.3:+47561290 MS.gene83205:CDS
ACCAGATGAGAGCGTGTCAA+CGG 0.566066 7.3:+47561044 MS.gene83205:CDS
ACATTGATGATGAAGAAGAA+TGG 0.574603 7.3:+47560221 MS.gene83205:CDS
ATGGCGTGCACGCATGAGCA+TGG 0.579124 7.3:+47561344 MS.gene83205:CDS
AAGGGAGGGATCGTGTGGAA+AGG 0.587033 7.3:+47561304 MS.gene83205:CDS
GTGCACGCATGAGCATGGCT+GGG 0.597641 7.3:+47561349 MS.gene83205:CDS
AGAAATGCAGTTGCTTGTGA+AGG 0.600882 7.3:+47561285 MS.gene83205:CDS
ATGCAGTTGCTTGTGAAGGG+AGG 0.602267 7.3:+47561289 MS.gene83205:CDS
GCTGCCACCTCAATTTCTGA+AGG 0.602543 7.3:+47560439 MS.gene83205:CDS
CTGAACAATTCCGCAACTGG+AGG 0.608240 7.3:-47560595 None:intergenic
GTGTATGTGCGAAACTAGAG+AGG 0.610106 7.3:-47560486 None:intergenic
ATTACTGTGCATGAAGAGAA+TGG 0.610201 7.3:+47561441 MS.gene83205:CDS
TGAAAATCCACCTCCAGTTG+CGG 0.610781 7.3:+47560585 MS.gene83205:CDS
AGATGAATTTAGTGATGTGG+TGG 0.611511 7.3:-47560362 None:intergenic
GTGAACCATTGAGACTCGCA+TGG 0.614247 7.3:-47560807 None:intergenic
AAAGAGAAGTTGAGTTGACT+CGG 0.617772 7.3:-47560629 None:intergenic
TTATGAACACCAACAACGAA+TGG 0.618414 7.3:+47560263 MS.gene83205:CDS
AGGAGATGAATTTAGTGATG+TGG 0.635063 7.3:-47560365 None:intergenic
GTCATTGAATTGTGACTCAG+AGG 0.645367 7.3:+47560981 MS.gene83205:CDS
AGGCAAAATGGATTATGCGA+AGG 0.646229 7.3:+47560459 MS.gene83205:CDS
AGCACCATTAGTTTCACGTG+TGG 0.653510 7.3:+47561155 MS.gene83205:CDS
GAAATGCAGTTGCTTGTGAA+GGG 0.657022 7.3:+47561286 MS.gene83205:CDS
TGACGAAGAAGTGTGTCACA+TGG 0.664504 7.3:-47561072 None:intergenic
GTAACTTCAAGACTCATCCA+AGG 0.665089 7.3:+47561411 MS.gene83205:CDS
TTGATTTCAGATTGTCATCG+TGG 0.675782 7.3:-47560867 None:intergenic
TTTGCCTTCAGAAATTGAGG+TGG 0.677928 7.3:-47560443 None:intergenic
GTTGAAGATCACGACTGTGA+CGG 0.707788 7.3:+47560837 MS.gene83205:CDS
TTGTGAAGGGAGGGATCGTG+TGG 0.715844 7.3:+47561299 MS.gene83205:CDS
ACTGTGCATGAAGAGAATGG+TGG 0.760237 7.3:+47561444 MS.gene83205:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTATCGAATTTTAGTTTT+GGG - chr7.3:47560516-47560535 None:intergenic 20.0%
! AAAATTCGATAGTTGGTTAT+TGG + chr7.3:47560522-47560541 MS.gene83205:CDS 25.0%
! CAAAACTAAAATTCGATAGT+TGG + chr7.3:47560515-47560534 MS.gene83205:CDS 25.0%
!! AAATCAATCAGAGAACTTTT+GGG + chr7.3:47560883-47560902 MS.gene83205:CDS 25.0%
!!! AATGAAATAGGTTTTTGGAT+GGG - chr7.3:47560328-47560347 None:intergenic 25.0%
!!! CAACTATCGAATTTTAGTTT+TGG - chr7.3:47560517-47560536 None:intergenic 25.0%
AAAGAAGGTGAGAATGAAAT+AGG - chr7.3:47560340-47560359 None:intergenic 30.0%
AGAATCTATTGAATTTGCGA+TGG + chr7.3:47561209-47561228 MS.gene83205:CDS 30.0%
GAGAGATTAAAGCTTGAAAA+AGG + chr7.3:47561246-47561265 MS.gene83205:CDS 30.0%
GTTACTAACTCAGTAACTTT+CGG - chr7.3:47561396-47561415 None:intergenic 30.0%
! GAAATCAATCAGAGAACTTT+TGG + chr7.3:47560882-47560901 MS.gene83205:CDS 30.0%
! GTGAGAATGAAATAGGTTTT+TGG - chr7.3:47560333-47560352 None:intergenic 30.0%
! GTTTTCCAAACAATCGTTAA+TGG + chr7.3:47560414-47560433 MS.gene83205:CDS 30.0%
!! ACATTGATGATGAAGAAGAA+TGG + chr7.3:47560221-47560240 MS.gene83205:CDS 30.0%
!! ATGAAATAGGTTTTTGGATG+GGG - chr7.3:47560327-47560346 None:intergenic 30.0%
!! GAATGAAATAGGTTTTTGGA+TGG - chr7.3:47560329-47560348 None:intergenic 30.0%
!! GTGGATTTTGATATTGGTAA+TGG + chr7.3:47560754-47560773 MS.gene83205:CDS 30.0%
!! TGTGTTGTGGATTTTGATAT+TGG + chr7.3:47560748-47560767 MS.gene83205:CDS 30.0%
AATCGGGTTTGAATTCAAAC+TGG + chr7.3:47560927-47560946 MS.gene83205:CDS 35.0%
AGATGAATTTAGTGATGTGG+TGG - chr7.3:47560365-47560384 None:intergenic 35.0%
AGCATCCATTAACGATTGTT+TGG - chr7.3:47560422-47560441 None:intergenic 35.0%
AGGAGATGAATTTAGTGATG+TGG - chr7.3:47560368-47560387 None:intergenic 35.0%
ATTACTGTGCATGAAGAGAA+TGG + chr7.3:47561441-47561460 MS.gene83205:CDS 35.0%
GAGTTAGTTCGATGAGATTT+AGG - chr7.3:47560957-47560976 None:intergenic 35.0%
TGTGAAGAATCTTTGTGTTG+TGG + chr7.3:47560735-47560754 MS.gene83205:CDS 35.0%
TTATGAACACCAACAACGAA+TGG + chr7.3:47560263-47560282 MS.gene83205:CDS 35.0%
TTGATTTCAGATTGTCATCG+TGG - chr7.3:47560870-47560889 None:intergenic 35.0%
! CATTTTGCCTTCAGAAATTG+AGG - chr7.3:47560449-47560468 None:intergenic 35.0%
! CTCAATTTCTGAAGGCAAAA+TGG + chr7.3:47560447-47560466 MS.gene83205:CDS 35.0%
! CTTTGTTGTATCTTGGAATG+CGG - chr7.3:47560713-47560732 None:intergenic 35.0%
! TCACACTCTTTGTTGTATCT+TGG - chr7.3:47560720-47560739 None:intergenic 35.0%
!! AAAGAGAAGTTGAGTTGACT+CGG - chr7.3:47560632-47560651 None:intergenic 35.0%
!! AATGGTCACTAGTTGTTTCT+GGG + chr7.3:47560239-47560258 MS.gene83205:CDS 35.0%
!! TGGAAAGGTGTGAAGTTTTT+GGG + chr7.3:47561319-47561338 MS.gene83205:CDS 35.0%
AAGCTGAGAGTTTAGGAATC+GGG + chr7.3:47560911-47560930 MS.gene83205:CDS 40.0%
AGAAATGCAGTTGCTTGTGA+AGG + chr7.3:47561285-47561304 MS.gene83205:CDS 40.0%
CTCTCAATGTTGCATCTTCT+TGG + chr7.3:47561493-47561512 MS.gene83205:CDS 40.0%
GAAATGCAGTTGCTTGTGAA+GGG + chr7.3:47561286-47561305 MS.gene83205:CDS 40.0%
GAAGCTGAGAGTTTAGGAAT+CGG + chr7.3:47560910-47560929 MS.gene83205:CDS 40.0%
GCTGGTGAAACAATAGATGA+AGG - chr7.3:47560388-47560407 None:intergenic 40.0%
GTAACTTCAAGACTCATCCA+AGG + chr7.3:47561411-47561430 MS.gene83205:CDS 40.0%
GTCATTGAATTGTGACTCAG+AGG + chr7.3:47560981-47561000 MS.gene83205:CDS 40.0%
TCCAAAACTGTGACAATGCT+TGG - chr7.3:47561114-47561133 None:intergenic 40.0%
TGTTTGGAAAACTCAGAAGC+TGG - chr7.3:47560406-47560425 None:intergenic 40.0%
TTGCTTCAAGCTCTCTTTCA+TGG + chr7.3:47560663-47560682 MS.gene83205:CDS 40.0%
TTTGCCTTCAGAAATTGAGG+TGG - chr7.3:47560446-47560465 None:intergenic 40.0%
! AGTGATGTGGTGGAAAAAGA+AGG - chr7.3:47560355-47560374 None:intergenic 40.0%
! GAATGGTCACTAGTTGTTTC+TGG + chr7.3:47560238-47560257 MS.gene83205:CDS 40.0%
! TGTTTCTGACCATTCGTTGT+TGG - chr7.3:47560275-47560294 None:intergenic 40.0%
!! AGGCAAAATGGATTATGCGA+AGG + chr7.3:47560459-47560478 MS.gene83205:CDS 40.0%
!! GGTGTGAAGTTTTTGGGAAA+TGG + chr7.3:47561325-47561344 MS.gene83205:CDS 40.0%
!! GTGGAAAGGTGTGAAGTTTT+TGG + chr7.3:47561318-47561337 MS.gene83205:CDS 40.0%
!! TCCAAGCATTGTCACAGTTT+TGG + chr7.3:47561110-47561129 MS.gene83205:CDS 40.0%
ACTGTGCATGAAGAGAATGG+TGG + chr7.3:47561444-47561463 MS.gene83205:CDS 45.0%
GCACAGTAATTCTGCTACCT+TGG - chr7.3:47561431-47561450 None:intergenic 45.0%
GGCTTCAAGGATTGCGATAT+TGG - chr7.3:47560692-47560711 None:intergenic 45.0%
GTCGTGATCTTCAACATTGC+TGG - chr7.3:47560832-47560851 None:intergenic 45.0%
GTGTATGTGCGAAACTAGAG+AGG - chr7.3:47560489-47560508 None:intergenic 45.0%
GTTGAAGATCACGACTGTGA+CGG + chr7.3:47560837-47560856 MS.gene83205:CDS 45.0%
TATCTTGGAATGCGGCTTCA+AGG - chr7.3:47560705-47560724 None:intergenic 45.0%
TCAGCCACACGTGAAACTAA+TGG - chr7.3:47561162-47561181 None:intergenic 45.0%
TGAAAATCCACCTCCAGTTG+CGG + chr7.3:47560585-47560604 MS.gene83205:CDS 45.0%
TTGCTGAACAATTCCGCAAC+TGG - chr7.3:47560601-47560620 None:intergenic 45.0%
! AGCACCATTAGTTTCACGTG+TGG + chr7.3:47561155-47561174 MS.gene83205:CDS 45.0%
! ATGGTGGAGTTTGCTTTGGT+TGG + chr7.3:47561460-47561479 MS.gene83205:CDS 45.0%
! GAGAATGGTGGAGTTTGCTT+TGG + chr7.3:47561456-47561475 MS.gene83205:CDS 45.0%
! GGAGTTTGCTTTGGTTGGAT+GGG + chr7.3:47561465-47561484 MS.gene83205:CDS 45.0%
! TGACGAAGAAGTGTGTCACA+TGG - chr7.3:47561075-47561094 None:intergenic 45.0%
! TGGAGTTTGCTTTGGTTGGA+TGG + chr7.3:47561464-47561483 MS.gene83205:CDS 45.0%
AACAATTCCGCAACTGGAGG+TGG - chr7.3:47560595-47560614 None:intergenic 50.0%
ATGCAGTTGCTTGTGAAGGG+AGG + chr7.3:47561289-47561308 MS.gene83205:CDS 50.0%
CTGAACAATTCCGCAACTGG+AGG - chr7.3:47560598-47560617 None:intergenic 50.0%
GAACTCCATGCGAGTCTCAA+TGG + chr7.3:47560802-47560821 MS.gene83205:CDS 50.0%
GCTGCCACCTCAATTTCTGA+AGG + chr7.3:47560439-47560458 MS.gene83205:CDS 50.0%
GGTCGAGAAGCTGAGAGTTT+AGG + chr7.3:47560904-47560923 MS.gene83205:CDS 50.0%
GTGAACCATTGAGACTCGCA+TGG - chr7.3:47560810-47560829 None:intergenic 50.0%
TGAGACTCGCATGGAGTTCT+TGG - chr7.3:47560801-47560820 None:intergenic 50.0%
TGCAGTTGCTTGTGAAGGGA+GGG + chr7.3:47561290-47561309 MS.gene83205:CDS 50.0%
! ACCAGATGAGAGCGTGTCAA+CGG + chr7.3:47561044-47561063 MS.gene83205:CDS 50.0%
AAGGGAGGGATCGTGTGGAA+AGG + chr7.3:47561304-47561323 MS.gene83205:CDS 55.0%
GCATGAGCATGGCTGGGTTT+AGG + chr7.3:47561355-47561374 MS.gene83205:CDS 55.0%
TCCGTTGACACGCTCTCATC+TGG - chr7.3:47561048-47561067 None:intergenic 55.0%
TTGTGAAGGGAGGGATCGTG+TGG + chr7.3:47561299-47561318 MS.gene83205:CDS 55.0%
ATGGCGTGCACGCATGAGCA+TGG + chr7.3:47561344-47561363 MS.gene83205:CDS 60.0%
GTGCACGCATGAGCATGGCT+GGG + chr7.3:47561349-47561368 MS.gene83205:CDS 60.0%
CGTGCACGCATGAGCATGGC+TGG + chr7.3:47561348-47561367 MS.gene83205:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 47560193 47561521 47560193 ID=MS.gene83205
chr7.3 mRNA 47560193 47561521 47560193 ID=MS.gene83205.t1;Parent=MS.gene83205
chr7.3 exon 47560193 47561521 47560193 ID=MS.gene83205.t1.exon1;Parent=MS.gene83205.t1
chr7.3 CDS 47560193 47561521 47560193 ID=cds.MS.gene83205.t1;Parent=MS.gene83205.t1
Gene Sequence

>MS.gene83205

ATGAATTGGAAAAAAAAGCTTCATGAAGACATTGATGATGAAGAAGAATGGTCACTAGTTGTTTCTGGGATTATGAACACCAACAACGAATGGTCAGAAACAATTCGTAATTTGAATAGTTTCGAGCAAAACCCCATCCAAAAACCTATTTCATTCTCACCTTCTTTTTCCACCACATCACTAAATTCATCTCCTTCATCTATTGTTTCACCAGCTTCTGAGTTTTCCAAACAATCGTTAATGGATGCTGCCACCTCAATTTCTGAAGGCAAAATGGATTATGCGAAGGAGATCCTCTCTAGTTTCGCACATACACAAAACCCAAAACTAAAATTCGATAGTTGGTTATTGGATTGCATTGCTTCAGCGCTGAAATCGCGTCTGTATAACATTGAAAATCCACCTCCAGTTGCGGAATTGTTCAGCAAAGAACATGCCGAGTCAACTCAACTTCTCTTTGATCACTCTCTTTGCTTCAAGCTCTCTTTCATGGCTGCCAATATCGCAATCCTTGAAGCCGCATTCCAAGATACAACAAAGAGTGTGAAGAATCTTTGTGTTGTGGATTTTGATATTGGTAATGGAAAACAATACATCAATCTGCTCCAAGAACTCCATGCGAGTCTCAATGGTTCACCAGCAATGTTGAAGATCACGACTGTGACGGTGAACATCCACGATGACAATCTGAAATCAATCAGAGAACTTTTGGGTCGAGAAGCTGAGAGTTTAGGAATCGGGTTTGAATTCAAACTGGTGAACCTAAATCTCATCGAACTAACTCGCGAGTCATTGAATTGTGACTCAGAGGATATTCTTGCTGTGAATTTCGCGTTTAATCTATACAAAATACCAGATGAGAGCGTGTCAACGGAGAATCCATGTGACACACTTCTTCGTCAAGTGAAATCACTCTCTCCAAGCATTGTCACAGTTTTGGAGCAAGAGATGAATACAAACACAGCACCATTAGTTTCACGTGTGGCTGAAACTTGTTCTTATTACAGCACATTGCTAGAATCTATTGAATTTGCGATGGATCATGATAGTTATGAGAGATTAAAGCTTGAAAAAGGACTGAGCATGAAGATGAGAAATGCAGTTGCTTGTGAAGGGAGGGATCGTGTGGAAAGGTGTGAAGTTTTTGGGAAATGGCGTGCACGCATGAGCATGGCTGGGTTTAGGTTAAACTCGATGAGTCCGAAAGTTACTGAGTTAGTAACTTCAAGACTCATCCAAGGTAGCAGAATTACTGTGCATGAAGAGAATGGTGGAGTTTGCTTTGGTTGGATGGGAAAAACTCTCAATGTTGCATCTTCTTGGTGTTAA
Protein sequence

>MS.gene83205.t1

MNWKKKLHEDIDDEEEWSLVVSGIMNTNNEWSETIRNLNSFEQNPIQKPISFSPSFSTTSLNSSPSSIVSPASEFSKQSLMDAATSISEGKMDYAKEILSSFAHTQNPKLKFDSWLLDCIASALKSRLYNIENPPPVAELFSKEHAESTQLLFDHSLCFKLSFMAANIAILEAAFQDTTKSVKNLCVVDFDIGNGKQYINLLQELHASLNGSPAMLKITTVTVNIHDDNLKSIRELLGREAESLGIGFEFKLVNLNLIELTRESLNCDSEDILAVNFAFNLYKIPDESVSTENPCDTLLRQVKSLSPSIVTVLEQEMNTNTAPLVSRVAETCSYYSTLLESIEFAMDHDSYERLKLEKGLSMKMRNAVACEGRDRVERCEVFGKWRARMSMAGFRLNSMSPKVTELVTSRLIQGSRITVHEENGGVCFGWMGKTLNVASSWC