Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83376.t1 | AES81629.1 | 94.2 | 86 | 5 | 0 | 38 | 123 | 11 | 96 | 1.20E-37 | 166 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83376.t1 | F4JZT3 | 39.2 | 120 | 69 | 2 | 16 | 135 | 18 | 133 | 1.1e-13 | 77.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83376.t1 | G7KYG8 | 94.2 | 86 | 5 | 0 | 38 | 123 | 11 | 96 | 8.9e-38 | 166.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene83376.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83376.t1 | MTR_7g098860 | 94.186 | 86 | 5 | 0 | 38 | 123 | 11 | 96 | 1.53e-54 | 166 |
| MS.gene83376.t1 | MTR_7g098910 | 47.500 | 120 | 58 | 3 | 16 | 135 | 22 | 136 | 2.47e-25 | 94.0 |
| MS.gene83376.t1 | MTR_7g099500 | 43.902 | 123 | 65 | 2 | 16 | 138 | 11 | 129 | 1.22e-24 | 92.0 |
| MS.gene83376.t1 | MTR_8g063580 | 45.545 | 101 | 52 | 2 | 16 | 114 | 12 | 111 | 9.56e-18 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83376.t1 | AT5G26594 | 39.167 | 120 | 69 | 2 | 16 | 135 | 18 | 133 | 4.02e-19 | 78.2 |
| MS.gene83376.t1 | AT3G04280 | 41.414 | 99 | 57 | 1 | 16 | 113 | 22 | 120 | 5.46e-15 | 67.4 |
| MS.gene83376.t1 | AT3G04280 | 41.414 | 99 | 57 | 1 | 16 | 113 | 22 | 120 | 5.46e-15 | 67.4 |
| MS.gene83376.t1 | AT3G04280 | 41.414 | 99 | 57 | 1 | 16 | 113 | 22 | 120 | 5.46e-15 | 67.4 |
Find 32 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCGGGACTCTGTGATGATA+TGG | 0.319136 | 7.4:-11593254 | None:intergenic |
| GCCCGTCATGGATGGAATTA+CGG | 0.406314 | 7.4:+11593448 | MS.gene83376:CDS |
| CTGCATATTCATCATTAGTA+AGG | 0.410681 | 7.4:-11593744 | None:intergenic |
| AGAGATATCTTGACATTACT+TGG | 0.437895 | 7.4:+11593329 | MS.gene83376:CDS |
| GAATACCCTGGCCAGATTGT+TGG | 0.444431 | 7.4:+11593709 | MS.gene83376:CDS |
| AACCGTGCCAATGAGGTCTA+AGG | 0.458884 | 7.4:-11593387 | None:intergenic |
| TATGCAGAATTTCGCAATGC+AGG | 0.461823 | 7.4:+11593760 | MS.gene83376:CDS |
| GACGTGAGACACCAACAATC+TGG | 0.490889 | 7.4:-11593720 | None:intergenic |
| GATGATCTACATCATGAAGA+TGG | 0.493143 | 7.4:+11593787 | MS.gene83376:CDS |
| CCCGCGTGGATTAAAAGTAC+TGG | 0.511605 | 7.4:+11593271 | MS.gene83376:CDS |
| GAGACACCAACAATCTGGCC+AGG | 0.513873 | 7.4:-11593715 | None:intergenic |
| AAAGAAGCCTTAGACCTCAT+TGG | 0.516268 | 7.4:+11593380 | MS.gene83376:CDS |
| AGGATCATGCCCGTCATGGA+TGG | 0.527719 | 7.4:+11593440 | MS.gene83376:CDS |
| TATCTTGACATTACTTGGTG+TGG | 0.530817 | 7.4:+11593334 | MS.gene83376:CDS |
| TTTCAACATATGATAGTGCA+AGG | 0.532595 | 7.4:-11593815 | None:intergenic |
| AAAAGGTCATGCTAGTTGAC+AGG | 0.534345 | 7.4:-11593859 | None:intergenic |
| GGCTAGGATCATGCCCGTCA+TGG | 0.548604 | 7.4:+11593436 | MS.gene83376:CDS |
| ACCGTAATTCCATCCATGAC+GGG | 0.549235 | 7.4:-11593449 | None:intergenic |
| TGGATTAAAAGTACTGGTTG+TGG | 0.556379 | 7.4:+11593277 | MS.gene83376:CDS |
| ATGCAGAATTTCGCAATGCA+GGG | 0.581025 | 7.4:+11593761 | MS.gene83376:CDS |
| TGATCCTAGCCATGAGAATG+AGG | 0.586084 | 7.4:-11593424 | None:intergenic |
| TAAATTCGACCTCATTCTCA+TGG | 0.588335 | 7.4:+11593415 | MS.gene83376:CDS |
| TCGACCTCATTCTCATGGCT+AGG | 0.592585 | 7.4:+11593420 | MS.gene83376:CDS |
| AACCGTAATTCCATCCATGA+CGG | 0.593130 | 7.4:-11593450 | None:intergenic |
| TACGTTCAACCGTGCCAATG+AGG | 0.601575 | 7.4:-11593394 | None:intergenic |
| AGCCTTAGACCTCATTGGCA+CGG | 0.608494 | 7.4:+11593385 | MS.gene83376:CDS |
| AGATGATGATCTTGTTCGAA+TGG | 0.623757 | 7.4:+11593301 | MS.gene83376:CDS |
| TTCAACATATGATAGTGCAA+GGG | 0.630612 | 7.4:-11593814 | None:intergenic |
| AGACACCAACAATCTGGCCA+GGG | 0.657108 | 7.4:-11593714 | None:intergenic |
| GGGACTCTGTGATGATATGG+AGG | 0.663830 | 7.4:-11593251 | None:intergenic |
| CTGCGCGATTTAGAATACCC+TGG | 0.695519 | 7.4:+11593697 | MS.gene83376:CDS |
| TATCATCACAGAGTCCCGCG+TGG | 0.741838 | 7.4:+11593257 | MS.gene83376:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATGAATGATTATATGCT+AGG | - | chr7.4:11593626-11593645 | None:intergenic | 20.0% |
| !! | TAATTACATACATACATACA+CGG | + | chr7.4:11593484-11593503 | MS.gene83376:intron | 20.0% |
| !!! | AATTGCTTTAAACTAATTCA+TGG | - | chr7.4:11593522-11593541 | None:intergenic | 20.0% |
| !!! | ATTGCTTTAAACTAATTCAT+GGG | - | chr7.4:11593521-11593540 | None:intergenic | 20.0% |
| !!! | GTTGAAACTAATTTTGAAAA+GGG | + | chr7.4:11593831-11593850 | MS.gene83376:CDS | 20.0% |
| !!! | TGTTGAAACTAATTTTGAAA+AGG | + | chr7.4:11593830-11593849 | MS.gene83376:CDS | 20.0% |
| AGAGATATCTTGACATTACT+TGG | + | chr7.4:11593329-11593348 | MS.gene83376:CDS | 30.0% | |
| ATGAACAGAAAGAAAATGCA+TGG | + | chr7.4:11593584-11593603 | MS.gene83376:intron | 30.0% | |
| CTGCATATTCATCATTAGTA+AGG | - | chr7.4:11593747-11593766 | None:intergenic | 30.0% | |
| TGAACAGAAAGAAAATGCAT+GGG | + | chr7.4:11593585-11593604 | MS.gene83376:intron | 30.0% | |
| ! | TTCAACATATGATAGTGCAA+GGG | - | chr7.4:11593817-11593836 | None:intergenic | 30.0% |
| ! | TTTCAACATATGATAGTGCA+AGG | - | chr7.4:11593818-11593837 | None:intergenic | 30.0% |
| AGATGATGATCTTGTTCGAA+TGG | + | chr7.4:11593301-11593320 | MS.gene83376:CDS | 35.0% | |
| GAACAGAAAGAAAATGCATG+GGG | + | chr7.4:11593586-11593605 | MS.gene83376:intron | 35.0% | |
| GATGATCTACATCATGAAGA+TGG | + | chr7.4:11593787-11593806 | MS.gene83376:CDS | 35.0% | |
| TAAATTCGACCTCATTCTCA+TGG | + | chr7.4:11593415-11593434 | MS.gene83376:CDS | 35.0% | |
| ! | TATCTTGACATTACTTGGTG+TGG | + | chr7.4:11593334-11593353 | MS.gene83376:CDS | 35.0% |
| !! | TGGATTAAAAGTACTGGTTG+TGG | + | chr7.4:11593277-11593296 | MS.gene83376:CDS | 35.0% |
| AAAGAAGCCTTAGACCTCAT+TGG | + | chr7.4:11593380-11593399 | MS.gene83376:CDS | 40.0% | |
| AACCGTAATTCCATCCATGA+CGG | - | chr7.4:11593453-11593472 | None:intergenic | 40.0% | |
| ATGCAGAATTTCGCAATGCA+GGG | + | chr7.4:11593761-11593780 | MS.gene83376:CDS | 40.0% | |
| GAAACTCACGCAGTCAAAAA+TGG | + | chr7.4:11593356-11593375 | MS.gene83376:CDS | 40.0% | |
| TATGCAGAATTTCGCAATGC+AGG | + | chr7.4:11593760-11593779 | MS.gene83376:CDS | 40.0% | |
| ! | ACCAGTACTTTTAATCCACG+CGG | - | chr7.4:11593275-11593294 | None:intergenic | 40.0% |
| ACCGTAATTCCATCCATGAC+GGG | - | chr7.4:11593452-11593471 | None:intergenic | 45.0% | |
| CATTCATATGTGTGCACTGC+AGG | + | chr7.4:11593663-11593682 | MS.gene83376:intron | 45.0% | |
| GTCATGGATGGAATTACGGT+TGG | + | chr7.4:11593452-11593471 | MS.gene83376:intron | 45.0% | |
| TGATCCTAGCCATGAGAATG+AGG | - | chr7.4:11593427-11593446 | None:intergenic | 45.0% | |
| ! | CCAGTACTTTTAATCCACGC+GGG | - | chr7.4:11593274-11593293 | None:intergenic | 45.0% |
| AACCGTGCCAATGAGGTCTA+AGG | - | chr7.4:11593390-11593409 | None:intergenic | 50.0% | |
| AGACACCAACAATCTGGCCA+GGG | - | chr7.4:11593717-11593736 | None:intergenic | 50.0% | |
| AGCCTTAGACCTCATTGGCA+CGG | + | chr7.4:11593385-11593404 | MS.gene83376:CDS | 50.0% | |
| GAATACCCTGGCCAGATTGT+TGG | + | chr7.4:11593709-11593728 | MS.gene83376:CDS | 50.0% | |
| GACGTGAGACACCAACAATC+TGG | - | chr7.4:11593723-11593742 | None:intergenic | 50.0% | |
| GCCCGTCATGGATGGAATTA+CGG | + | chr7.4:11593448-11593467 | MS.gene83376:CDS | 50.0% | |
| GGGACTCTGTGATGATATGG+AGG | - | chr7.4:11593254-11593273 | None:intergenic | 50.0% | |
| TACGTTCAACCGTGCCAATG+AGG | - | chr7.4:11593397-11593416 | None:intergenic | 50.0% | |
| TCGACCTCATTCTCATGGCT+AGG | + | chr7.4:11593420-11593439 | MS.gene83376:CDS | 50.0% | |
| ! | CCCGCGTGGATTAAAAGTAC+TGG | + | chr7.4:11593271-11593290 | MS.gene83376:CDS | 50.0% |
| ! | CTGCGCGATTTAGAATACCC+TGG | + | chr7.4:11593697-11593716 | MS.gene83376:CDS | 50.0% |
| AGGATCATGCCCGTCATGGA+TGG | + | chr7.4:11593440-11593459 | MS.gene83376:CDS | 55.0% | |
| CGCGGGACTCTGTGATGATA+TGG | - | chr7.4:11593257-11593276 | None:intergenic | 55.0% | |
| GAGACACCAACAATCTGGCC+AGG | - | chr7.4:11593718-11593737 | None:intergenic | 55.0% | |
| TATCATCACAGAGTCCCGCG+TGG | + | chr7.4:11593257-11593276 | MS.gene83376:CDS | 55.0% | |
| GGCTAGGATCATGCCCGTCA+TGG | + | chr7.4:11593436-11593455 | MS.gene83376:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 11593236 | 11593870 | 11593236 | ID=MS.gene83376 |
| chr7.4 | mRNA | 11593236 | 11593870 | 11593236 | ID=MS.gene83376.t1;Parent=MS.gene83376 |
| chr7.4 | exon | 11593236 | 11593469 | 11593236 | ID=MS.gene83376.t1.exon1;Parent=MS.gene83376.t1 |
| chr7.4 | CDS | 11593236 | 11593469 | 11593236 | ID=cds.MS.gene83376.t1;Parent=MS.gene83376.t1 |
| chr7.4 | exon | 11593685 | 11593870 | 11593685 | ID=MS.gene83376.t1.exon2;Parent=MS.gene83376.t1 |
| chr7.4 | CDS | 11593685 | 11593870 | 11593685 | ID=cds.MS.gene83376.t1;Parent=MS.gene83376.t1 |
| Gene Sequence |
| Protein sequence |
