AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83575

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83575.t1 MTR_2g104560 92.174 230 15 3 6 234 2 229 2.24e-152 423
MS.gene83575.t1 MTR_2g104550 46.798 203 93 3 37 231 118 313 1.37e-51 170
MS.gene83575.t1 MTR_8g009020 46.231 199 94 3 45 233 131 326 6.18e-51 169
MS.gene83575.t1 MTR_4g009540 47.692 195 86 3 47 230 142 331 4.63e-50 166
MS.gene83575.t1 MTR_4g009110 47.396 192 88 3 47 230 164 350 6.60e-48 161
MS.gene83575.t1 MTR_4g009540 49.425 174 72 3 47 209 142 310 6.66e-44 150
MS.gene83575.t1 MTR_8g009010 40.625 224 90 4 9 225 80 267 1.25e-43 149
MS.gene83575.t1 MTR_2g104490 45.128 195 95 3 37 226 117 304 1.89e-43 149
MS.gene83575.t1 MTR_5g014600 43.316 187 85 5 56 225 148 330 1.37e-41 144
MS.gene83575.t1 MTR_2g104590 43.056 216 91 10 18 225 28 219 2.40e-41 141
MS.gene83575.t1 MTR_5g014520 42.553 188 94 4 50 230 150 330 9.34e-41 142
MS.gene83575.t1 MTR_2g104500 39.535 215 122 4 18 230 64 272 1.50e-40 140
MS.gene83575.t1 MTR_2g010450 43.258 178 90 4 54 225 174 346 4.32e-40 141
MS.gene83575.t1 MTR_2g104530 42.268 194 104 4 35 226 83 270 5.04e-40 139
MS.gene83575.t1 MTR_0250s0040 37.768 233 118 8 16 231 40 262 5.49e-39 136
MS.gene83575.t1 MTR_5g014640 41.304 184 101 4 48 229 145 323 5.54e-39 137
MS.gene83575.t1 MTR_0246s0020 41.667 204 101 7 38 231 181 376 7.96e-39 138
MS.gene83575.t1 MTR_7g080780 45.745 188 92 4 47 233 141 319 1.97e-38 136
MS.gene83575.t1 MTR_7g080780 45.745 188 92 4 47 233 154 332 3.29e-38 135
MS.gene83575.t1 MTR_2g104490 44.944 178 86 3 37 209 117 287 1.14e-37 133
MS.gene83575.t1 MTR_4g092700 40.449 178 90 4 56 225 157 326 2.79e-37 133
MS.gene83575.t1 MTR_2g104650 43.541 209 93 6 31 225 67 264 7.75e-37 130
MS.gene83575.t1 MTR_0011s0260 37.288 177 109 2 56 231 182 357 2.54e-36 131
MS.gene83575.t1 MTR_8g069740 42.439 205 93 5 18 208 72 265 3.99e-36 129
MS.gene83575.t1 MTR_6g047570 39.109 202 114 4 34 231 137 333 9.65e-36 129
MS.gene83575.t1 MTR_4g097940 39.785 186 102 5 50 231 148 327 1.87e-35 128
MS.gene83575.t1 MTR_0011s0210 37.288 177 109 2 56 231 182 357 3.53e-35 128
MS.gene83575.t1 MTR_4g067010 36.082 194 119 2 38 231 130 318 5.31e-35 127
MS.gene83575.t1 MTR_4g066460 39.894 188 108 2 44 231 144 326 3.42e-34 125
MS.gene83575.t1 MTR_0246s0050 34.419 215 116 4 35 231 111 318 5.05e-34 124
MS.gene83575.t1 MTR_4g098035 34.419 215 116 4 35 231 132 339 6.83e-34 124
MS.gene83575.t1 MTR_4g097920 37.968 187 104 5 50 231 133 312 9.46e-34 123
MS.gene83575.t1 MTR_4g097920 37.968 187 104 5 50 231 148 327 1.59e-33 123
MS.gene83575.t1 MTR_4g066380 36.181 199 99 4 44 231 142 323 1.12e-32 121
MS.gene83575.t1 MTR_0246s0020 40.761 184 91 7 38 211 181 356 3.61e-32 120
MS.gene83575.t1 MTR_5g014560 36.224 196 111 4 42 230 116 304 6.77e-32 118
MS.gene83575.t1 MTR_4g097950 40.212 189 90 4 58 231 71 251 1.38e-29 111
MS.gene83575.t1 MTR_4g009510 37.725 167 92 4 60 225 45 200 7.32e-26 100
MS.gene83575.t1 MTR_7g451650 30.667 225 123 5 1 225 1 192 2.00e-25 99.0
MS.gene83575.t1 MTR_4g009420 30.131 229 119 7 1 225 1 192 4.11e-25 98.2
MS.gene83575.t1 MTR_7g050350 36.429 140 79 2 86 225 1 130 1.18e-22 90.1
MS.gene83575.t1 MTR_7g451650 36.429 140 79 2 86 225 1 130 1.18e-22 90.1
MS.gene83575.t1 MTR_7g451650 30.986 213 114 5 1 213 1 180 9.68e-22 89.4
MS.gene83575.t1 MTR_4g009420 30.415 217 110 7 1 213 1 180 3.96e-21 87.8
MS.gene83575.t1 MTR_6g047550 49.367 79 39 1 34 112 151 228 1.34e-17 79.7
MS.gene83575.t1 MTR_5g014550 36.434 129 68 4 87 208 54 175 7.60e-14 68.2
MS.gene83575.t1 MTR_8g027495 23.563 174 124 3 58 231 459 623 5.53e-13 68.2
MS.gene83575.t1 MTR_1g072320 28.777 139 91 2 58 188 471 609 5.84e-13 68.2
MS.gene83575.t1 MTR_7g117670 25.294 170 118 4 58 227 403 563 7.20e-13 67.8
MS.gene83575.t1 MTR_3g030700 25.000 224 153 6 15 233 160 373 1.14e-11 63.9
MS.gene83575.t1 MTR_2g038040 22.581 217 142 4 30 233 34 237 1.31e-11 62.8
MS.gene83575.t1 MTR_5g030430 29.787 188 107 8 58 228 494 673 3.32e-11 62.8
MS.gene83575.t1 MTR_8g098275 47.273 55 29 0 58 112 245 299 7.69e-11 61.6
MS.gene83575.t1 MTR_8g098275 47.273 55 29 0 58 112 309 363 9.50e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83575.t1 AT4G37850 40.306 196 102 5 38 225 133 321 5.56e-35 127
MS.gene83575.t1 AT4G37850 40.306 196 102 5 38 225 133 321 5.56e-35 127
MS.gene83575.t1 AT2G22760 37.634 186 99 5 40 217 106 282 1.26e-34 125
MS.gene83575.t1 AT2G22760 38.202 178 93 5 40 209 106 274 3.18e-32 119
MS.gene83575.t1 AT2G22770 38.542 192 91 5 55 225 130 315 1.92e-31 117
MS.gene83575.t1 AT2G22750 37.688 199 96 5 49 231 118 304 2.01e-29 112
MS.gene83575.t1 AT2G22750 37.688 199 96 5 49 231 142 328 3.04e-29 112
MS.gene83575.t1 AT2G22750 37.688 199 96 5 49 231 146 332 3.69e-29 112
MS.gene83575.t1 AT2G22750 37.688 199 95 6 49 231 118 303 5.84e-28 108
MS.gene83575.t1 AT2G22750 37.688 199 95 6 49 231 146 331 9.34e-28 108
MS.gene83575.t1 AT4G09820 32.394 142 76 3 43 184 349 470 1.51e-15 75.5
MS.gene83575.t1 AT5G57150 25.822 213 125 7 1 197 9 204 2.09e-13 67.4
MS.gene83575.t1 AT5G57150 25.822 213 125 7 1 197 9 204 2.34e-13 67.4
MS.gene83575.t1 AT4G29930 24.554 224 137 7 3 218 10 209 2.96e-13 67.0
MS.gene83575.t1 AT5G57150 25.822 213 125 7 1 197 9 204 4.32e-13 67.4
MS.gene83575.t1 AT5G57150 25.217 230 140 7 1 215 9 221 5.34e-13 66.6
MS.gene83575.t1 AT4G29930 23.394 218 144 5 3 218 10 206 6.09e-13 66.2
MS.gene83575.t1 AT5G57150 25.943 212 125 7 1 197 9 203 1.48e-12 65.1
MS.gene83575.t1 AT5G46760 25.824 182 104 3 58 213 416 592 1.60e-12 66.6
MS.gene83575.t1 AT1G63650 29.412 153 86 4 54 186 402 552 3.10e-12 65.9
MS.gene83575.t1 AT1G63650 29.412 153 86 4 54 186 402 552 3.10e-12 65.9
MS.gene83575.t1 AT1G63650 29.412 153 86 4 54 186 402 552 3.10e-12 65.9
MS.gene83575.t1 AT5G41315 26.752 157 90 3 54 186 446 601 3.46e-12 65.9
MS.gene83575.t1 AT5G41315 26.752 157 90 3 54 186 443 598 3.55e-12 65.9
MS.gene83575.t1 AT5G41315 26.752 157 90 3 54 186 438 593 3.68e-12 65.9
MS.gene83575.t1 AT4G29930 23.469 196 128 4 3 196 10 185 4.62e-12 63.9
MS.gene83575.t1 AT1G12860 24.713 174 123 3 58 224 268 440 6.70e-12 64.7
MS.gene83575.t1 AT1G01260 27.586 174 100 5 54 227 430 577 7.81e-12 64.7
MS.gene83575.t1 AT1G01260 27.586 174 100 5 54 227 430 577 7.81e-12 64.7
MS.gene83575.t1 AT1G01260 27.586 174 100 5 54 227 430 577 7.81e-12 64.7
MS.gene83575.t1 AT2G46510 39.394 66 40 0 58 123 396 461 8.45e-12 64.7
MS.gene83575.t1 AT4G17880 28.415 183 105 7 58 228 417 585 3.39e-11 62.8
MS.gene83575.t1 AT4G29930 23.013 239 148 8 3 218 10 235 3.51e-11 61.6
MS.gene83575.t1 AT1G12860 26.144 153 105 3 58 203 268 419 5.30e-11 62.0
MS.gene83575.t1 AT1G32640 25.000 140 98 2 58 193 453 589 6.29e-11 62.0

Find 50 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCCACGTAATGATAATT+TGG 0.206550 2.2:+1128417 MS.gene83575:CDS
ATGCCAATGGCCTCCACTTC+AGG 0.214258 2.2:-1128460 None:intergenic
CCATGTGTAGCAGCATTATT+AGG 0.265823 2.2:-1126699 None:intergenic
GGAGAATTGGTTCTCTAATA+TGG 0.295421 2.2:+1126482 MS.gene83575:CDS
CAGAAGCATCCAACAATAAT+AGG 0.297499 2.2:+1126657 MS.gene83575:CDS
ACATAATGTCAGAGAGAAAA+AGG 0.300304 2.2:+1126763 MS.gene83575:CDS
GAACAATGTCCTATTATTGT+TGG 0.353321 2.2:-1126666 None:intergenic
ATAGACAAGGTTTCTGTACT+TGG 0.374394 2.2:+1128277 MS.gene83575:CDS
ACCAGCAGTGTGCTTCCATT+TGG 0.381044 2.2:+1128589 MS.gene83575:CDS
TGGAGAATTGGTGGGAGAAT+TGG 0.381146 2.2:+1126469 MS.gene83575:CDS
GTTCAAACTATTGTCAATGC+TGG 0.402081 2.2:+1128543 MS.gene83575:CDS
CTATACCTAACATGGAGAAT+TGG 0.414546 2.2:+1126457 None:intergenic
AGAGAGATTCATAGCACTTT+CGG 0.417342 2.2:+1126800 MS.gene83575:CDS
GGCAGATGGGTGAGGAATAC+AGG 0.417383 2.2:+1128728 MS.gene83575:intron
ATTGATGACAAAGTTGAAAC+AGG 0.417457 2.2:+1128765 MS.gene83575:CDS
TTCAGGGAGGAGCACATGAT+TGG 0.447101 2.2:-1128443 None:intergenic
TGCCAATGGCCTCCACTTCA+GGG 0.452486 2.2:-1128459 None:intergenic
AAACATTACCTTAGCAATGA+TGG 0.455229 2.2:-1128632 None:intergenic
CTCCCTGAAGTGGAGGCCAT+TGG 0.457623 2.2:+1128457 MS.gene83575:CDS
CTACACATGGAAAGAATAAG+AGG 0.462322 2.2:+1126712 MS.gene83575:CDS
TGTTGGATGCTTCTGATTGA+AGG 0.471742 2.2:-1126649 None:intergenic
CTTCTCTGATTCTATGCCAA+TGG 0.479471 2.2:-1128473 None:intergenic
TCCAAATGGAAGCACACTGC+TGG 0.484948 2.2:-1128590 None:intergenic
TTAATATCTATAGGATATGA+AGG 0.498841 2.2:+1126614 MS.gene83575:intron
TAAAGAACGCATATCAATGC+TGG 0.499426 2.2:+1128327 MS.gene83575:CDS
AGCAATGATGGTAATGTTGA+GGG 0.515073 2.2:-1128620 None:intergenic
CCTAATAATGCTGCTACACA+TGG 0.515165 2.2:+1126699 MS.gene83575:CDS
TACCTAACATGGAGAATTGG+TGG 0.525198 2.2:+1126460 None:intergenic
TATGATACCCGGCTTGAAGA+AGG 0.532421 2.2:+1126824 MS.gene83575:CDS
AAATGGAAGCACACTGCTGG+TGG 0.542174 2.2:-1128587 None:intergenic
GCACTTTCGGCTATGATACC+CGG 0.547609 2.2:+1126813 MS.gene83575:CDS
TCATGTGCTCCTCCCTGAAG+TGG 0.548063 2.2:+1128447 MS.gene83575:CDS
GGAACTGACCTTCTTCAAGC+CGG 0.556400 2.2:-1126832 None:intergenic
TAGCAATGATGGTAATGTTG+AGG 0.557198 2.2:-1128621 None:intergenic
TAAGAGGGTGAGAAGCTCAT+GGG 0.563045 2.2:+1126728 MS.gene83575:CDS
CAACATTACCATCATTGCTA+AGG 0.570598 2.2:+1128624 MS.gene83575:CDS
ATAAGAGGGTGAGAAGCTCA+TGG 0.579940 2.2:+1126727 MS.gene83575:CDS
GAACTGACCTTCTTCAAGCC+GGG 0.582458 2.2:-1126831 None:intergenic
ATCAACTGCGAGAAACATGA+AGG 0.584639 2.2:+1128514 MS.gene83575:CDS
CACTGCTGGTGGAAACATAG+AGG 0.588212 2.2:-1128576 None:intergenic
ACCTAACATGGAGAATTGGT+GGG 0.590580 2.2:+1126461 None:intergenic
TGTGCTCCTCCCTGAAGTGG+AGG 0.603078 2.2:+1128450 MS.gene83575:CDS
AGAAGCTCATGGGAGATACA+AGG 0.603853 2.2:+1126738 MS.gene83575:CDS
GAATCCAAATTATCATTACG+TGG 0.646914 2.2:-1128421 None:intergenic
CATTGGCATAGAATCAGAGA+AGG 0.647651 2.2:+1128474 MS.gene83575:CDS
TACACATGGAAAGAATAAGA+GGG 0.651853 2.2:+1126713 MS.gene83575:CDS
TAATATCTATAGGATATGAA+GGG 0.651855 2.2:+1126615 MS.gene83575:intron
TGGAGCAACAATGTTATGAG+AGG 0.657981 2.2:+1128347 MS.gene83575:CDS
TTTACTTGTATAGATAGACA+AGG 0.695353 2.2:+1128264 MS.gene83575:intron
CAATGGCCTCCACTTCAGGG+AGG 0.758935 2.2:-1128456 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAATAATAAATTAACAC+AGG + chr2.2:1127875-1127894 MS.gene83575:intron 10.0%
!!! AAATTTTTTAATAGAGATTA+TGG - chr2.2:1128392-1128411 None:intergenic 10.0%
!! GAAAAAAATTAAAGTTGTAT+TGG + chr2.2:1127180-1127199 MS.gene83575:intron 15.0%
!! TACAATATAATTGAATTACT+TGG - chr2.2:1127349-1127368 None:intergenic 15.0%
!! TTGATAAAATGTTAATTACA+TGG + chr2.2:1128696-1128715 MS.gene83575:intron 15.0%
!!! ATAATTAAGCTTGTTAATTT+TGG - chr2.2:1127741-1127760 None:intergenic 15.0%
!!! ATTAACTTTTTATAATGGAT+AGG + chr2.2:1126954-1126973 MS.gene83575:intron 15.0%
!!! CTTTTTTTGACAATATTTAA+TGG + chr2.2:1127293-1127312 MS.gene83575:intron 15.0%
!! AAACATGGAAATACTAAATA+TGG + chr2.2:1126910-1126929 MS.gene83575:intron 20.0%
!! AAATAAAGAGTGAGAATAAA+AGG + chr2.2:1128074-1128093 MS.gene83575:intron 20.0%
!! AATAAAGAGTGAGAATAAAA+GGG + chr2.2:1128075-1128094 MS.gene83575:intron 20.0%
!! ATTAATTTAGTTGAGATACA+TGG - chr2.2:1127587-1127606 None:intergenic 20.0%
!! GACATAAAATAAATTAACAC+AGG + chr2.2:1127805-1127824 MS.gene83575:intron 20.0%
!! TAATATCTATAGGATATGAA+GGG + chr2.2:1126615-1126634 MS.gene83575:intron 20.0%
!! TATAGATATTAAACACAACA+AGG - chr2.2:1126606-1126625 None:intergenic 20.0%
!! TTAATATCTATAGGATATGA+AGG + chr2.2:1126614-1126633 MS.gene83575:intron 20.0%
!! TTGTAAGATGTTTAAATAGT+GGG - chr2.2:1127392-1127411 None:intergenic 20.0%
!! TTGTTGTGTTTAATATCTAT+AGG + chr2.2:1126605-1126624 MS.gene83575:intron 20.0%
!! TTTGTAAGATGTTTAAATAG+TGG - chr2.2:1127393-1127412 None:intergenic 20.0%
!!! ATTTTTAGTATAGTCATTAG+AGG + chr2.2:1127670-1127689 MS.gene83575:intron 20.0%
!!! GACAATATTTAATGGATATA+TGG + chr2.2:1127301-1127320 MS.gene83575:intron 20.0%
!!! TTAATTACATGGATTTTATG+TGG + chr2.2:1128707-1128726 MS.gene83575:intron 20.0%
!!! TTCAAGTATTTTTGTTGATA+AGG + chr2.2:1128126-1128145 MS.gene83575:intron 20.0%
!!! TTTTTACACTCTTAAGTTTT+AGG + chr2.2:1128194-1128213 MS.gene83575:intron 20.0%
! AATACATTGATAAGCCAAAA+AGG + chr2.2:1127633-1127652 MS.gene83575:intron 25.0%
! AGTATTTATTGAGGTAAAGT+TGG + chr2.2:1127155-1127174 MS.gene83575:intron 25.0%
! TACAAGATTAATTCAGTTCA+TGG + chr2.2:1127465-1127484 MS.gene83575:intron 25.0%
! TGTAAGATGTTTAAATAGTG+GGG - chr2.2:1127391-1127410 None:intergenic 25.0%
! TTGTATTTAGTAGTGATACA+TGG + chr2.2:1127364-1127383 MS.gene83575:intron 25.0%
! TTTACTTGTATAGATAGACA+AGG + chr2.2:1128264-1128283 MS.gene83575:intron 25.0%
!! AAAAGTTGGAGTATTTATTG+AGG + chr2.2:1127146-1127165 MS.gene83575:intron 25.0%
!! AGATTTTCTTTGTACCTTTT+TGG - chr2.2:1127650-1127669 None:intergenic 25.0%
!! CTAAATATGGATTAAACATG+AGG + chr2.2:1126923-1126942 MS.gene83575:intron 25.0%
!! TTGGCTTATCAATGTATTTT+GGG - chr2.2:1127631-1127650 None:intergenic 25.0%
!!! AAGTGACAATCATTTTGAAA+CGG + chr2.2:1127245-1127264 MS.gene83575:intron 25.0%
!!! GTTTTTTGTAGATCAAAACA+TGG + chr2.2:1126895-1126914 MS.gene83575:intron 25.0%
!!! TGTCTTGTTCTTTTAAAGTA+AGG - chr2.2:1127092-1127111 None:intergenic 25.0%
!!! TTTGGCTTATCAATGTATTT+TGG - chr2.2:1127632-1127651 None:intergenic 25.0%
AAACATTACCTTAGCAATGA+TGG - chr2.2:1128635-1128654 None:intergenic 30.0%
ACATAATGTCAGAGAGAAAA+AGG + chr2.2:1126763-1126782 MS.gene83575:CDS 30.0%
ATTATGACATGTGACTTATG+AGG + chr2.2:1128163-1128182 MS.gene83575:intron 30.0%
GAACAATGTCCTATTATTGT+TGG - chr2.2:1126669-1126688 None:intergenic 30.0%
GAATCCAAATTATCATTACG+TGG - chr2.2:1128424-1128443 None:intergenic 30.0%
GTAAATGTGAGTAACAAAGA+AGG + chr2.2:1127783-1127802 MS.gene83575:intron 30.0%
GTATATATTGAAGAAGAAGC+AGG - chr2.2:1126856-1126875 None:intergenic 30.0%
TACACATGGAAAGAATAAGA+GGG + chr2.2:1126713-1126732 MS.gene83575:CDS 30.0%
TCATCCACGTAATGATAATT+TGG + chr2.2:1128417-1128436 MS.gene83575:CDS 30.0%
TTTGTTACTCACATTTACAG+AGG - chr2.2:1127781-1127800 None:intergenic 30.0%
! ATTGATGACAAAGTTGAAAC+AGG + chr2.2:1128765-1128784 MS.gene83575:CDS 30.0%
! TAAGTTTGAGTTCTTATGCT+TGG - chr2.2:1127715-1127734 None:intergenic 30.0%
!!! TTGAGGGTATTTTTTCCAAA+TGG - chr2.2:1128607-1128626 None:intergenic 30.0%
AGAGAGATTCATAGCACTTT+CGG + chr2.2:1126800-1126819 MS.gene83575:CDS 35.0%
ATAGACAAGGTTTCTGTACT+TGG + chr2.2:1128277-1128296 MS.gene83575:CDS 35.0%
ATCAATTTCGAGATGAAGCA+AGG + chr2.2:1127605-1127624 MS.gene83575:intron 35.0%
ATTGTGTGAGAGAGAAAAGT+TGG + chr2.2:1127132-1127151 MS.gene83575:intron 35.0%
CAACATTACCATCATTGCTA+AGG + chr2.2:1128624-1128643 MS.gene83575:CDS 35.0%
CTACACATGGAAAGAATAAG+AGG + chr2.2:1126712-1126731 MS.gene83575:CDS 35.0%
GAGAAAAAGGAGACAAGAAA+TGG + chr2.2:1126776-1126795 MS.gene83575:CDS 35.0%
GTTCAAACTATTGTCAATGC+TGG + chr2.2:1128543-1128562 MS.gene83575:CDS 35.0%
TAAAGAACGCATATCAATGC+TGG + chr2.2:1128327-1128346 MS.gene83575:CDS 35.0%
TCAGTTCATGGCAAAAATTG+TGG + chr2.2:1127477-1127496 MS.gene83575:intron 35.0%
TTAACACAGGTGATTTCACA+TGG + chr2.2:1127818-1127837 MS.gene83575:intron 35.0%
TTCCTTAATCCGTATGCAAT+TGG - chr2.2:1127026-1127045 None:intergenic 35.0%
! AAAGCGACACTCATTTTGAA+AGG - chr2.2:1127001-1127020 None:intergenic 35.0%
! AGCAATGATGGTAATGTTGA+GGG - chr2.2:1128623-1128642 None:intergenic 35.0%
! ATGGATTTTATGTGGCAGAT+GGG + chr2.2:1128715-1128734 MS.gene83575:intron 35.0%
! ATTGCATACGGATTAAGGAA+TGG + chr2.2:1127026-1127045 MS.gene83575:intron 35.0%
! GGAGAATTGGTTCTCTAATA+TGG + chr2.2:1126482-1126501 MS.gene83575:CDS 35.0%
! TAGCAATGATGGTAATGTTG+AGG - chr2.2:1128624-1128643 None:intergenic 35.0%
!! CAGAAGCATCCAACAATAAT+AGG + chr2.2:1126657-1126676 MS.gene83575:CDS 35.0%
!!! GACAATCATTTTGAAACGGA+TGG + chr2.2:1127249-1127268 MS.gene83575:intron 35.0%
ATCAACTGCGAGAAACATGA+AGG + chr2.2:1128514-1128533 MS.gene83575:CDS 40.0%
CATTGGCATAGAATCAGAGA+AGG + chr2.2:1128474-1128493 MS.gene83575:CDS 40.0%
CCATGTGTAGCAGCATTATT+AGG - chr2.2:1126702-1126721 None:intergenic 40.0%
CCTAATAATGCTGCTACACA+TGG + chr2.2:1126699-1126718 MS.gene83575:CDS 40.0%
CTTCTCTGATTCTATGCCAA+TGG - chr2.2:1128476-1128495 None:intergenic 40.0%
GTTACTCACATTTACAGAGG+AGG - chr2.2:1127778-1127797 None:intergenic 40.0%
TCGCTTTAGCCAATTGCATA+CGG + chr2.2:1127014-1127033 MS.gene83575:intron 40.0%
TGGAGCAACAATGTTATGAG+AGG + chr2.2:1128347-1128366 MS.gene83575:CDS 40.0%
TGTTGGATGCTTCTGATTGA+AGG - chr2.2:1126652-1126671 None:intergenic 40.0%
! AGCCAATTGCATACGGATTA+AGG + chr2.2:1127021-1127040 MS.gene83575:intron 40.0%
! CATGGATTTTATGTGGCAGA+TGG + chr2.2:1128714-1128733 MS.gene83575:intron 40.0%
!! CGACACTCATTTTGAAAGGA+TGG - chr2.2:1126997-1127016 None:intergenic 40.0%
!! CGGATGGAGTACTATTTTAG+TGG + chr2.2:1127265-1127284 MS.gene83575:intron 40.0%
AGAAGCTCATGGGAGATACA+AGG + chr2.2:1126738-1126757 MS.gene83575:CDS 45.0%
ATAAGAGGGTGAGAAGCTCA+TGG + chr2.2:1126727-1126746 MS.gene83575:CDS 45.0%
GAGTTCTTATGCTTGGAGCT+TGG - chr2.2:1127708-1127727 None:intergenic 45.0%
TAAGAGGGTGAGAAGCTCAT+GGG + chr2.2:1126728-1126747 MS.gene83575:CDS 45.0%
TGGAGAATTGGTGGGAGAAT+TGG + chr2.2:1126469-1126488 MS.gene83575:CDS 45.0%
TTCTTATGCTTGGAGCTTGG+TGG - chr2.2:1127705-1127724 None:intergenic 45.0%
! TATGATACCCGGCTTGAAGA+AGG + chr2.2:1126824-1126843 MS.gene83575:CDS 45.0%
! TTTTATGTGGCAGATGGGTG+AGG + chr2.2:1128720-1128739 MS.gene83575:intron 45.0%
!!! ATGAAATTATTTTTATAAAA+TGG - chr2.2:1126523-1126542 None:intergenic 5.0%
!!! ATTATATTAACTTTTTATAA+TGG + chr2.2:1126949-1126968 MS.gene83575:intron 5.0%
ACCAGCAGTGTGCTTCCATT+TGG + chr2.2:1128589-1128608 MS.gene83575:CDS 50.0%
CACTGCTGGTGGAAACATAG+AGG - chr2.2:1128579-1128598 None:intergenic 50.0%
GAACTGACCTTCTTCAAGCC+GGG - chr2.2:1126834-1126853 None:intergenic 50.0%
GCACTTTCGGCTATGATACC+CGG + chr2.2:1126813-1126832 MS.gene83575:CDS 50.0%
GGAACTGACCTTCTTCAAGC+CGG - chr2.2:1126835-1126854 None:intergenic 50.0%
! AAATGGAAGCACACTGCTGG+TGG - chr2.2:1128590-1128609 None:intergenic 50.0%
! TCCAAATGGAAGCACACTGC+TGG - chr2.2:1128593-1128612 None:intergenic 50.0%
! TTCAGGGAGGAGCACATGAT+TGG - chr2.2:1128446-1128465 None:intergenic 50.0%
ATGCCAATGGCCTCCACTTC+AGG - chr2.2:1128463-1128482 None:intergenic 55.0%
GGCAGATGGGTGAGGAATAC+AGG + chr2.2:1128728-1128747 MS.gene83575:intron 55.0%
TCATGTGCTCCTCCCTGAAG+TGG + chr2.2:1128447-1128466 MS.gene83575:CDS 55.0%
TGCCAATGGCCTCCACTTCA+GGG - chr2.2:1128462-1128481 None:intergenic 55.0%
CAATGGCCTCCACTTCAGGG+AGG - chr2.2:1128459-1128478 None:intergenic 60.0%
CTCCCTGAAGTGGAGGCCAT+TGG + chr2.2:1128457-1128476 MS.gene83575:CDS 60.0%
TGTGCTCCTCCCTGAAGTGG+AGG + chr2.2:1128450-1128469 MS.gene83575:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 1126468 1128813 1126468 ID=MS.gene83575
chr2.2 mRNA 1126468 1128813 1126468 ID=MS.gene83575.t1;Parent=MS.gene83575
chr2.2 exon 1126468 1126503 1126468 ID=MS.gene83575.t1.exon1;Parent=MS.gene83575.t1
chr2.2 CDS 1126468 1126503 1126468 ID=cds.MS.gene83575.t1;Parent=MS.gene83575.t1
chr2.2 exon 1126627 1126845 1126627 ID=MS.gene83575.t1.exon2;Parent=MS.gene83575.t1
chr2.2 CDS 1126627 1126845 1126627 ID=cds.MS.gene83575.t1;Parent=MS.gene83575.t1
chr2.2 exon 1128277 1128645 1128277 ID=MS.gene83575.t1.exon3;Parent=MS.gene83575.t1
chr2.2 CDS 1128277 1128645 1128277 ID=cds.MS.gene83575.t1;Parent=MS.gene83575.t1
chr2.2 exon 1128733 1128813 1128733 ID=MS.gene83575.t1.exon4;Parent=MS.gene83575.t1
chr2.2 CDS 1128733 1128813 1128733 ID=cds.MS.gene83575.t1;Parent=MS.gene83575.t1
Gene Sequence

>MS.gene83575

ATGGAGAATTGGTGGGAGAATTGGTTCTCTAATATGGTAATTAATTAACTAGCCATTTTATAAAAATAATTTCATTGTCAAGTTAATATCAATTTTGTCAAATTATATGTATATGCAATGATAATATTTCTATCTCCTTGTTGTGTTTAATATCTATAGGATATGAAGGGTAGTGATAACTCCTTCAATCAGAAGCATCCAACAATAATAGGACATTGTTCTAATTCAGTGCCTAATAATGCTGCTACACATGGAAAGAATAAGAGGGTGAGAAGCTCATGGGAGATACAAGGTCACATAATGTCAGAGAGAAAAAGGAGACAAGAAATGGCAGAGAGATTCATAGCACTTTCGGCTATGATACCCGGCTTGAAGAAGGTCAGTTCCTGCTTCTTCTTCAATATATACACTATATATGCAACATTCTGTTTTTTGTAGATCAAAACATGGAAATACTAAATATGGATTAAACATGAGGATTATTATATTAACTTTTTATAATGGATAGGTTTCTTTGTTTTATACTCCATCCTTTCAAAATGAGTGTCGCTTTAGCCAATTGCATACGGATTAAGGAATGGAATAAAGTAGTCAAATGTCATAACAATTTTACTAAAATAACCTTACTTTAAAAGAACAAGACAAACTTAAAGTTGCATTGAAAATTGTGTGAGAGAGAAAAGTTGGAGTATTTATTGAGGTAAAGTTGGAAGAAAAAAATTAAAGTTGTATTGGAAATGTAAAGTGACATTCATTTTAAAACAATTTTTTTTTTTAAAGTGACAATCATTTTGAAACGGATGGAGTACTATTTTAGTGGATATGCTTTTTTTGACAATATTTAATGGATATATGGTTAACAATGTAAAAATACTTTACCAAGTAATTCAATTATATTGTATTTAGTAGTGATACATGGACCCCACTATTTAAACATCTTACAAAATTTAATCAAAAAAAAAAAAAAATCTTACAAAATGTGATTAATTCAATTGAATACAAGATTAATTCAGTTCATGGCAAAAATTGTGGTTAATTCAATTTCAAGAATTAAACATGAATTTCAATGTGAACTATTTAATCAACTAAATTAATCACATTTTTGCATGTGAGTAACCATGTATCTCAACTAAATTAATCAATTTCGAGATGAAGCAAGGCCCAAAATACATTGATAAGCCAAAAAGGTACAAAGAAAATCTATTTTTAGTATAGTCATTAGAGGCAACTGAGACCACCAAGCTCCAAGCATAAGAACTCAAACTTAAATCCAAAATTAACAAGCTTAATTATCAGCACGTTAATTTCCTCCTCTGTAAATGTGAGTAACAAAGAAGGACATAAAATAAATTAACACAGGTGATTTCACATGGTGATTTCTAAATTATGAAATATAATTTATATAAAATTAAATAATAAATTAACACAGGTGCAACAACAATTGAGATATTTATTGTCATGCAATGCTTTTTCACTCTAAGTTTTTTTTTAAAAAGAATAAATTGAAACATTTAATTACTAACTAAAGTTAAATATAGTGATTTTGCATTTGTTTAATTTGAGAGAAATTGTCAATAAAAGAATATTTTTCTCTTGCATTTGTTTCAAATAAAGAGTGAGAATAAAAGGGAACGTATGATAGAAGAATGATGAAGATATTCAAGTATTTTTGTTGATAAGGTTTAGTAAATGTACATTATGACATGTGACTTATGAGGTGAGATAATTTTTACACTCTTAAGTTTTAGGTGAGATTCTTTATTGTGAGATTGATATATAACAATGTCATTAAATATTTTACTTGTATAGATAGACAAGGTTTCTGTACTTGGTGAAGCTATTAATTACGTGAAACAACTTAAAGAACGCATATCAATGCTGGAGCAACAATGTTATGAGAGGAATAAAAAAAGTAGCAAGTCCATAATCTCTATTAAAAAATTTCAATCTCATCCACGTAATGATAATTTGGATTCCAATCATGTGCTCCTCCCTGAAGTGGAGGCCATTGGCATAGAATCAGAGAAGGAACTAGTACTTATTAAAATCAACTGCGAGAAACATGAAGGCATTTTGTTCAAACTATTGTCAATGCTGGAAAATATGCACCTCTATGTTTCCACCAGCAGTGTGCTTCCATTTGGAAAAAATACCCTCAACATTACCATCATTGCTAAGGTAATGTTTTTATGTTTCAAATATGAATACTTGTTTGAAATTTTGAAGCATTGATAAAATGTTAATTACATGGATTTTATGTGGCAGATGGGTGAGGAATACAGGATAACAATAGAAGAATTGATGACAAAGTTGAAACAGGATGTTTTGAAGTTATATGACATGTAA
Protein sequence

>MS.gene83575.t1

MENWWENWFSNMDMKGSDNSFNQKHPTIIGHCSNSVPNNAATHGKNKRVRSSWEIQGHIMSERKRRQEMAERFIALSAMIPGLKKIDKVSVLGEAINYVKQLKERISMLEQQCYERNKKSSKSIISIKKFQSHPRNDNLDSNHVLLPEVEAIGIESEKELVLIKINCEKHEGILFKLLSMLENMHLYVSTSSVLPFGKNTLNITIIAKMGEEYRITIEELMTKLKQDVLKLYDM