AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83869

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83869.t1 MTR_6g027460 92.167 383 15 3 1 383 1 368 0.0 684
MS.gene83869.t1 MTR_6g027450 73.569 367 59 6 1 367 1 329 0.0 521
MS.gene83869.t1 MTR_7g086780 50.263 380 136 10 15 383 10 347 2.71e-113 337
MS.gene83869.t1 MTR_7g010950 45.876 194 76 6 56 246 94 261 4.97e-43 156
MS.gene83869.t1 MTR_6g012450 32.951 349 165 10 55 383 96 395 6.30e-43 156
MS.gene83869.t1 MTR_4g082060 33.642 324 146 11 9 289 4 301 3.73e-41 151
MS.gene83869.t1 MTR_3g435480 37.358 265 111 10 50 281 98 340 5.38e-40 148
MS.gene83869.t1 MTR_8g044220 33.083 266 115 8 41 289 67 286 6.19e-36 136
MS.gene83869.t1 MTR_5g041420 53.509 114 44 2 55 162 99 209 2.96e-34 129
MS.gene83869.t1 MTR_5g041530 52.294 109 49 1 54 162 100 205 1.74e-30 120
MS.gene83869.t1 MTR_2g016030 77.419 62 14 0 89 150 39 100 3.42e-30 114
MS.gene83869.t1 MTR_5g041380 62.791 86 27 2 65 150 100 180 2.12e-29 117
MS.gene83869.t1 MTR_5g041400 57.778 90 32 2 61 150 89 172 8.76e-29 115
MS.gene83869.t1 MTR_8g068210 47.872 94 36 2 59 152 7 87 4.27e-24 102
MS.gene83869.t1 MTR_8g027295 60.317 63 25 0 92 154 19 81 1.35e-23 99.4
MS.gene83869.t1 MTR_4g022370 60.656 61 24 0 90 150 75 135 1.84e-23 100
MS.gene83869.t1 MTR_4g088580 44.762 105 48 2 56 152 8 110 2.24e-23 100
MS.gene83869.t1 MTR_4g461080 55.844 77 33 1 74 149 5 81 2.43e-23 98.6
MS.gene83869.t1 MTR_2g093220 55.263 76 33 1 76 150 18 93 4.90e-23 98.2
MS.gene83869.t1 MTR_3g091820 54.930 71 32 0 81 151 27 97 5.23e-23 98.2
MS.gene83869.t1 MTR_8g479350 52.703 74 35 0 97 170 68 141 9.74e-23 98.2
MS.gene83869.t1 MTR_2g096740 56.716 67 29 0 84 150 64 130 1.17e-22 97.4
MS.gene83869.t1 MTR_2g096740 56.716 67 29 0 84 150 49 115 1.39e-22 96.7
MS.gene83869.t1 MTR_4g063780 61.667 60 23 0 90 149 68 127 1.81e-22 97.4
MS.gene83869.t1 MTR_8g079060 42.623 122 63 3 97 218 29 143 2.15e-22 95.1
MS.gene83869.t1 MTR_4g089095 48.485 99 35 2 69 152 7 104 7.38e-22 95.1
MS.gene83869.t1 MTR_2g013370 67.241 58 18 1 95 152 44 100 1.38e-21 93.6
MS.gene83869.t1 MTR_2g059540 69.388 49 15 0 97 145 31 79 1.75e-21 91.7
MS.gene83869.t1 MTR_2g014060 54.688 64 29 0 85 148 13 76 2.14e-21 94.4
MS.gene83869.t1 MTR_1g077600 47.500 80 37 1 75 149 16 95 2.26e-21 93.2
MS.gene83869.t1 MTR_4g089095 69.643 56 16 1 97 152 16 70 2.44e-21 92.8
MS.gene83869.t1 MTR_1g077600 47.500 80 37 1 75 149 16 95 2.55e-21 93.2
MS.gene83869.t1 MTR_7g059400 53.521 71 31 1 82 150 59 129 2.77e-21 94.4
MS.gene83869.t1 MTR_8g015840 65.385 52 18 0 97 148 20 71 3.17e-21 91.3
MS.gene83869.t1 MTR_4g109980 64.151 53 19 0 95 147 38 90 5.47e-21 92.8
MS.gene83869.t1 MTR_7g024670 67.308 52 17 0 98 149 78 129 7.10e-21 93.6
MS.gene83869.t1 MTR_3g090430 54.167 72 31 1 81 150 33 104 2.77e-20 91.3
MS.gene83869.t1 MTR_5g031440 60.000 55 22 0 93 147 39 93 2.97e-20 91.3
MS.gene83869.t1 MTR_2g014170 67.347 49 16 0 97 145 45 93 6.83e-20 89.7
MS.gene83869.t1 MTR_3g077750 56.452 62 26 1 87 147 50 111 1.31e-19 89.4
MS.gene83869.t1 MTR_1g056810 65.385 52 18 0 99 150 14 65 1.61e-19 88.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83869.t1 AT3G47500 56.667 120 46 3 53 172 71 184 2.33e-39 145
MS.gene83869.t1 AT5G39660 44.828 174 61 6 63 221 92 245 9.17e-36 136
MS.gene83869.t1 AT5G39660 44.828 174 61 6 63 221 92 245 9.17e-36 136
MS.gene83869.t1 AT5G39660 44.828 174 61 6 63 221 92 245 9.17e-36 136
MS.gene83869.t1 AT5G62430 52.137 117 49 2 64 178 26 137 6.12e-35 130
MS.gene83869.t1 AT2G34140 58.065 93 26 1 71 150 19 111 8.42e-34 124
MS.gene83869.t1 AT1G26790 48.951 143 60 4 55 184 67 209 8.52e-34 129
MS.gene83869.t1 AT1G29160 78.333 60 13 0 91 150 56 115 2.81e-31 117
MS.gene83869.t1 AT1G69570 45.385 130 43 2 85 213 120 222 3.22e-31 122
MS.gene83869.t1 AT3G55370 57.143 70 30 0 93 162 72 141 1.85e-24 102
MS.gene83869.t1 AT3G55370 57.143 70 30 0 93 162 72 141 2.05e-24 103
MS.gene83869.t1 AT3G55370 57.143 70 30 0 93 162 117 186 4.49e-24 102
MS.gene83869.t1 AT2G28810 40.000 100 57 1 52 148 29 128 3.86e-23 99.0
MS.gene83869.t1 AT2G28810 40.000 100 57 1 52 148 46 145 4.84e-23 99.0
MS.gene83869.t1 AT5G60200 61.667 60 23 0 91 150 49 108 6.73e-23 97.1
MS.gene83869.t1 AT3G21270 69.811 53 16 0 97 149 29 81 9.88e-23 95.1
MS.gene83869.t1 AT2G37590 62.069 58 22 0 93 150 85 142 1.63e-22 97.4
MS.gene83869.t1 AT3G45610 45.055 91 42 2 93 175 36 126 2.11e-22 95.5
MS.gene83869.t1 AT1G51700 52.857 70 31 1 79 148 17 84 3.63e-22 93.6
MS.gene83869.t1 AT1G28310 64.286 56 20 0 97 152 27 82 1.11e-21 94.7
MS.gene83869.t1 AT4G24060 56.923 65 28 0 86 150 42 106 1.31e-21 95.1
MS.gene83869.t1 AT3G61850 61.017 59 23 0 92 150 57 115 1.34e-21 94.0
MS.gene83869.t1 AT3G61850 61.017 59 23 0 92 150 57 115 1.34e-21 94.0
MS.gene83869.t1 AT1G28310 64.286 56 20 0 97 152 51 106 1.36e-21 95.1
MS.gene83869.t1 AT3G61850 61.017 59 23 0 92 150 69 127 1.58e-21 94.0
MS.gene83869.t1 AT5G60850 66.667 54 18 0 97 150 51 104 1.84e-21 94.0
MS.gene83869.t1 AT3G61850 61.017 59 23 0 92 150 57 115 2.12e-21 94.0
MS.gene83869.t1 AT1G07640 66.038 53 18 0 97 149 21 73 2.26e-21 93.2
MS.gene83869.t1 AT3G50410 66.038 53 18 0 97 149 30 82 2.30e-21 92.8
MS.gene83869.t1 AT3G61850 61.017 59 23 0 92 150 69 127 2.59e-21 94.0
MS.gene83869.t1 AT2G28510 66.071 56 19 0 99 154 50 105 2.59e-21 93.2
MS.gene83869.t1 AT1G07640 67.273 55 17 1 97 150 77 131 3.42e-21 93.6
MS.gene83869.t1 AT5G02460 59.016 61 25 0 90 150 88 148 3.78e-21 94.7
MS.gene83869.t1 AT5G66940 53.425 73 27 1 84 156 26 91 4.16e-21 91.3
MS.gene83869.t1 AT1G07640 66.038 53 18 0 97 149 85 137 4.47e-21 93.6
MS.gene83869.t1 AT5G65590 64.815 54 19 0 97 150 41 94 5.69e-21 92.8
MS.gene83869.t1 AT4G00940 52.778 72 30 1 85 152 52 123 1.69e-20 91.3
MS.gene83869.t1 AT4G00940 52.778 72 30 1 85 152 52 123 1.69e-20 91.3
MS.gene83869.t1 AT4G00940 52.778 72 30 1 85 152 52 123 1.69e-20 91.3
MS.gene83869.t1 AT1G21340 42.553 94 53 1 99 191 40 133 4.79e-20 89.4
MS.gene83869.t1 AT5G62940 39.450 109 50 2 55 147 15 123 6.35e-20 90.5
MS.gene83869.t1 AT4G21050 47.436 78 39 1 77 152 2 79 7.16e-20 87.4
MS.gene83869.t1 AT4G38000 49.367 79 38 2 96 172 40 118 8.79e-20 88.2
MS.gene83869.t1 AT3G52440 63.462 52 19 0 99 150 27 78 2.11e-19 87.0
MS.gene83869.t1 AT1G64620 53.333 60 28 0 86 145 38 97 2.23e-19 89.0
MS.gene83869.t1 AT1G47655 65.306 49 17 0 97 145 29 77 2.99e-19 85.9
MS.gene83869.t1 AT3G52440 63.462 52 19 0 99 150 46 97 3.14e-19 87.0
MS.gene83869.t1 AT2G46590 58.491 53 22 0 92 144 63 115 1.15e-18 87.0
MS.gene83869.t1 AT2G46590 58.491 53 22 0 92 144 75 127 1.43e-18 86.7
MS.gene83869.t1 AT4G21040 48.611 72 37 0 99 170 27 98 8.91e-16 76.6
MS.gene83869.t1 AT4G21080 50.877 57 28 0 99 155 27 83 1.08e-14 73.6

Find 80 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGGTATTGTCCTAAAATT+TGG 0.197738 6.1:-9557072 MS.gene83869:CDS
CAATCTTGTCTGGTTTCTTT+AGG 0.212759 6.1:+9557394 None:intergenic
TAGAAACCAACGACTCTTCT+AGG 0.239536 6.1:-9557149 MS.gene83869:CDS
TTGGAACAATGTTACTTCTA+TGG 0.251370 6.1:-9556806 MS.gene83869:CDS
GGTAACAATTGTAGAGAAAA+TGG 0.277928 6.1:-9556970 MS.gene83869:CDS
GGTGAGTTCTTCTCAGATTC+AGG 0.283209 6.1:-9558535 MS.gene83869:CDS
AAGATTAAGCATTGAACCTT+TGG 0.298547 6.1:+9557023 None:intergenic
TCTTCTAGTTTCATGCTCTT+TGG 0.299226 6.1:-9558570 MS.gene83869:CDS
TTTAGGTGTTCAAATGATAA+TGG 0.318424 6.1:-9557090 MS.gene83869:CDS
GTCGGAGTTGTCACAGATAT+TGG 0.320960 6.1:-9557290 MS.gene83869:CDS
TTGTTCCAACTTTGATTCAT+TGG 0.340788 6.1:+9556820 None:intergenic
ATTTACCATGGTTGTAGAAT+TGG 0.341653 6.1:+9558512 None:intergenic
AAGCACTGTGGAAGATAATA+TGG 0.348391 6.1:-9557445 MS.gene83869:CDS
CCTTTGTATGGTAGTGGAAA+TGG 0.370217 6.1:-9556637 MS.gene83869:CDS
ATGCAGATGTGTAACCCTTA+TGG 0.380909 6.1:-9556772 MS.gene83869:CDS
AGAGTCGTTGGTTTCTAAAC+TGG 0.381811 6.1:+9557155 None:intergenic
GGACATTGAACAATCTTGTC+TGG 0.411323 6.1:+9557384 None:intergenic
ACAAATTGCAAGCCAATGTT+TGG 0.418330 6.1:+9556685 None:intergenic
GCTCTTTGGTAAGAAGATTC+CGG 0.421687 6.1:-9558556 MS.gene83869:CDS
TGCAGTAACTCAAAGCATAA+AGG 0.422749 6.1:-9557546 MS.gene83869:CDS
AGGTTTAGACCTATTCTGTT+CGG 0.445629 6.1:+9556876 None:intergenic
AATGCAACCATTTGAACCTA+TGG 0.446070 6.1:+9556606 None:intergenic
GTGTCACGCGCCGAGGGTTG+CGG 0.447216 6.1:-9556725 MS.gene83869:CDS
ACTAGTAATAGTTGTATCTC+AGG 0.449615 6.1:-9556565 MS.gene83869:CDS
AATTTGTTCCTGCATCAAAC+AGG 0.461385 6.1:-9556669 MS.gene83869:CDS
AGAAATGTTGCTGTCGGTGC+AGG 0.467099 6.1:-9557249 MS.gene83869:CDS
GTTCCATTCCTGTTTGATGC+AGG 0.467432 6.1:+9556661 None:intergenic
AGAGCTACCCTGCATCTTCA+TGG 0.473280 6.1:-9556849 MS.gene83869:CDS
TTGCATCAACATTTCTCTTG+TGG 0.474797 6.1:+9556995 None:intergenic
TATTGTCCTAAAATTTGGTC+GGG 0.484036 6.1:-9557067 MS.gene83869:CDS
TATTCGAATCCGAACAGAAT+AGG 0.487381 6.1:-9556885 MS.gene83869:CDS
GGAATTATCCATGAAGATGC+AGG 0.489315 6.1:+9556841 None:intergenic
GAAGTAGGATCAATGCCATA+AGG 0.489322 6.1:+9556757 None:intergenic
GGTAATGGCACACCAATCCT+TGG 0.490161 6.1:-9556544 MS.gene83869:CDS
AATAGTTGTATCTCAGGTAA+TGG 0.494093 6.1:-9556559 MS.gene83869:CDS
GGAACACCTTTGTATGGTAG+TGG 0.497692 6.1:-9556643 MS.gene83869:CDS
TTGCTGTCGGTGCAGGGAGA+CGG 0.499466 6.1:-9557242 MS.gene83869:CDS
TAATTCCAATGAATCAAAGT+TGG 0.500347 6.1:-9556825 MS.gene83869:CDS
TGCAGTGGTGTCACGCGCCG+AGG 0.505833 6.1:-9556732 MS.gene83869:CDS
ATTTCTCATTGTTCCACCGT+TGG 0.510921 6.1:+9557266 None:intergenic
GCAGTGGTGTCACGCGCCGA+GGG 0.511659 6.1:-9556731 MS.gene83869:CDS
AGGAATGGAACACCTTTGTA+TGG 0.533609 6.1:-9556649 MS.gene83869:CDS
TTGGTACCTAGAAGAGTCGT+TGG 0.535519 6.1:+9557143 None:intergenic
GTATTGTCCTAAAATTTGGT+CGG 0.547769 6.1:-9557068 MS.gene83869:CDS
ATTCAACATTTGAGCCATGT+TGG 0.548791 6.1:+9557124 None:intergenic
AATGTGTCCATAGGTTCAAA+TGG 0.549266 6.1:-9556613 MS.gene83869:CDS
GTTCCTGCATCAAACAGGAA+TGG 0.554691 6.1:-9556664 MS.gene83869:CDS
AAGAGAAATGTTGATGCAAG+TGG 0.555436 6.1:-9556991 MS.gene83869:CDS
TGTGTATCGTCTGTGACAAA+CGG 0.557160 6.1:-9556928 MS.gene83869:CDS
ACCTTTGGATTCATCGAGAG+AGG 0.559535 6.1:+9557038 None:intergenic
AAGTAGGATCAATGCCATAA+GGG 0.561604 6.1:+9556758 None:intergenic
AAATGGAGAGGAAGAATCAT+CGG 0.568618 6.1:-9556953 MS.gene83869:CDS
GGAAATGGAAATGTGTCCAT+AGG 0.578755 6.1:-9556622 MS.gene83869:CDS
TTGGTTTCTAAACTGGCGGT+CGG 0.586129 6.1:+9557162 None:intergenic
TGACACCACTGCATTGAAGT+AGG 0.587068 6.1:+9556742 None:intergenic
ATCAACCTCGGCATTTCTGT+CGG 0.588083 6.1:-9557308 MS.gene83869:CDS
ATGTTTGTAACCGCAACCCT+CGG 0.590311 6.1:+9556715 None:intergenic
TCAGGCCAATTCTACAACCA+TGG 0.595350 6.1:-9558517 MS.gene83869:intron
CACATGATTGTAGTGTCCGA+TGG 0.602575 6.1:-9557189 MS.gene83869:CDS
GTCGTTGGTTTCTAAACTGG+CGG 0.606694 6.1:+9557158 None:intergenic
GATGCAGGGTAGCTCTGCAT+AGG 0.608266 6.1:+9556856 None:intergenic
CCATTTCCACTACCATACAA+AGG 0.611279 6.1:+9556637 None:intergenic
CTCAAATGTTGAATCTGCTG+CGG 0.611992 6.1:-9557115 MS.gene83869:CDS
GCCTCTCTCGATGAATCCAA+AGG 0.612065 6.1:-9557039 MS.gene83869:CDS
TTACTAGTAGTAGATGAAGA+AGG 0.612171 6.1:+9556580 None:intergenic
GAATTATCCATGAAGATGCA+GGG 0.627674 6.1:+9556842 None:intergenic
CACAGATATTGGACCAACGG+TGG 0.629754 6.1:-9557279 MS.gene83869:CDS
TGAATCTGAGAAGAACTCAC+CGG 0.630634 6.1:+9558537 None:intergenic
TGTCACAGATATTGGACCAA+CGG 0.638912 6.1:-9557282 MS.gene83869:CDS
ACTGGCGGTCGGAATTCCAT+CGG 0.649455 6.1:+9557173 None:intergenic
TTGATCCTACTTCAATGCAG+TGG 0.649462 6.1:-9556747 MS.gene83869:CDS
GTGACAAACGGTCATACTCG+AGG 0.662893 6.1:-9556916 MS.gene83869:CDS
GAAATGTTGCTGTCGGTGCA+GGG 0.663844 6.1:-9557248 MS.gene83869:CDS
CAACTCCGACAGAAATGCCG+AGG 0.680790 6.1:+9557303 None:intergenic
ACTACAACGTCAATCAACCT+CGG 0.682406 6.1:-9557320 MS.gene83869:CDS
GACTCTTCTAGGTACCAACA+TGG 0.687547 6.1:-9557138 MS.gene83869:CDS
ACAATGAGAAATGTTGCTGT+CGG 0.691886 6.1:-9557255 MS.gene83869:CDS
CATCGGACACTACAATCATG+TGG 0.695881 6.1:+9557190 None:intergenic
CAATTGTAGAGAAAATGGAG+AGG 0.705347 6.1:-9556965 MS.gene83869:CDS
AAAAGTGAACACAAGCACTG+TGG 0.727270 6.1:-9557457 MS.gene83869:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTATTTTTGTTGTAT+TGG - chr6.1:9556720-9556739 MS.gene83869:CDS 10.0%
!!! TTTTTTATTTTTGTTGTATT+GGG - chr6.1:9556721-9556740 MS.gene83869:CDS 10.0%
!! AATTTAAATGTTTCAAAGAT+TGG + chr6.1:9556762-9556781 None:intergenic 15.0%
!! ATAAAAAAAACTCAAGAATA+TGG - chr6.1:9556825-9556844 MS.gene83869:CDS 15.0%
!! TAAAAAAACAACATAAACAA+TGG + chr6.1:9556683-9556702 None:intergenic 15.0%
!! TTTCATAAATATAACATGAT+TGG - chr6.1:9557202-9557221 MS.gene83869:CDS 15.0%
!!! TAAATTTGTTTAATTTTGCT+TGG - chr6.1:9557122-9557141 MS.gene83869:CDS 15.0%
!! AATTTAAGAAACTGAGTTTA+AGG - chr6.1:9556974-9556993 MS.gene83869:CDS 20.0%
!!! TTAAGAGTATTTGATCTATA+TGG - chr6.1:9556783-9556802 MS.gene83869:CDS 20.0%
! AAGTCATGAAGAATAAAGTT+TGG + chr6.1:9557532-9557551 None:intergenic 25.0%
! AATGGTATTGTCCTAAAATT+TGG - chr6.1:9558062-9558081 MS.gene83869:intron 25.0%
! ACAACATAAACAATGGTTAT+TGG + chr6.1:9556676-9556695 None:intergenic 25.0%
! CAACATAAACAATGGTTATT+GGG + chr6.1:9556675-9556694 None:intergenic 25.0%
! GATAAAATGAGTATTTACCA+TGG + chr6.1:9556637-9556656 None:intergenic 25.0%
! TAATTCCAATGAATCAAAGT+TGG - chr6.1:9558309-9558328 MS.gene83869:intron 25.0%
! TTTAGGTGTTCAAATGATAA+TGG - chr6.1:9558044-9558063 MS.gene83869:intron 25.0%
!!! TTATTTTTGTTGTATTGGGT+TGG - chr6.1:9556725-9556744 MS.gene83869:CDS 25.0%
AAAAACCATAAAATTCGACG+TGG + chr6.1:9557451-9557470 None:intergenic 30.0%
AATAGTTGTATCTCAGGTAA+TGG - chr6.1:9558575-9558594 MS.gene83869:CDS 30.0%
ACTAGTAATAGTTGTATCTC+AGG - chr6.1:9558569-9558588 MS.gene83869:CDS 30.0%
ATTTACCATGGTTGTAGAAT+TGG + chr6.1:9556625-9556644 None:intergenic 30.0%
GATCTGATTCATGTAAACAA+TGG - chr6.1:9556847-9556866 MS.gene83869:CDS 30.0%
GGTAACAATTGTAGAGAAAA+TGG - chr6.1:9558164-9558183 MS.gene83869:intron 30.0%
GTATTGTCCTAAAATTTGGT+CGG - chr6.1:9558066-9558085 MS.gene83869:intron 30.0%
TAGTGAGTTTGACAAAATCA+CGG - chr6.1:9557311-9557330 MS.gene83869:CDS 30.0%
TATTGTCCTAAAATTTGGTC+GGG - chr6.1:9558067-9558086 MS.gene83869:intron 30.0%
TGTGAAATAGACTGAAATTG+TGG - chr6.1:9557234-9557253 MS.gene83869:CDS 30.0%
TTACTAGTAGTAGATGAAGA+AGG + chr6.1:9558557-9558576 None:intergenic 30.0%
TTGTTCCAACTTTGATTCAT+TGG + chr6.1:9558317-9558336 None:intergenic 30.0%
! AACATTTTCACACCAAACAT+TGG - chr6.1:9558437-9558456 MS.gene83869:intron 30.0%
! GATTTTGTCAAACTCACTAT+GGG + chr6.1:9557310-9557329 None:intergenic 30.0%
! TGATTTTGTCAAACTCACTA+TGG + chr6.1:9557311-9557330 None:intergenic 30.0%
! TTGGAACAATGTTACTTCTA+TGG - chr6.1:9558328-9558347 MS.gene83869:intron 30.0%
!! AAGATTAAGCATTGAACCTT+TGG + chr6.1:9558114-9558133 None:intergenic 30.0%
!! TTCTCAATGTTTTGAGTATG+AGG + chr6.1:9557624-9557643 None:intergenic 30.0%
AAATGGAGAGGAAGAATCAT+CGG - chr6.1:9558181-9558200 MS.gene83869:intron 35.0%
AAGAGAAATGTTGATGCAAG+TGG - chr6.1:9558143-9558162 MS.gene83869:intron 35.0%
AAGTAGGATCAATGCCATAA+GGG + chr6.1:9558379-9558398 None:intergenic 35.0%
AATGCAACCATTTGAACCTA+TGG + chr6.1:9558531-9558550 None:intergenic 35.0%
AATGTGTCCATAGGTTCAAA+TGG - chr6.1:9558521-9558540 MS.gene83869:CDS 35.0%
ACAAATTGCAAGCCAATGTT+TGG + chr6.1:9558452-9558471 None:intergenic 35.0%
ACAATGAGAAATGTTGCTGT+CGG - chr6.1:9557879-9557898 MS.gene83869:intron 35.0%
AGCTGTCTTATGAAAGTAGT+TGG - chr6.1:9557004-9557023 MS.gene83869:CDS 35.0%
AGGTTTAGACCTATTCTGTT+CGG + chr6.1:9558261-9558280 None:intergenic 35.0%
ATTCAACATTTGAGCCATGT+TGG + chr6.1:9558013-9558032 None:intergenic 35.0%
CAATTGTAGAGAAAATGGAG+AGG - chr6.1:9558169-9558188 MS.gene83869:intron 35.0%
GAATTATCCATGAAGATGCA+GGG + chr6.1:9558295-9558314 None:intergenic 35.0%
TATGGGTAATTCCATAAACC+AGG + chr6.1:9557293-9557312 None:intergenic 35.0%
TATTCGAATCCGAACAGAAT+AGG - chr6.1:9558249-9558268 MS.gene83869:intron 35.0%
TGATTCATGTAAACAATGGC+TGG - chr6.1:9556851-9556870 MS.gene83869:CDS 35.0%
TGCAGTAACTCAAAGCATAA+AGG - chr6.1:9557588-9557607 MS.gene83869:intron 35.0%
TTGCATCAACATTTCTCTTG+TGG + chr6.1:9558142-9558161 None:intergenic 35.0%
! AAGCACTGTGGAAGATAATA+TGG - chr6.1:9557689-9557708 MS.gene83869:intron 35.0%
! AATTTGTTCCTGCATCAAAC+AGG - chr6.1:9558465-9558484 MS.gene83869:intron 35.0%
! ATGTACCACGTCGAATTTTA+TGG - chr6.1:9557443-9557462 MS.gene83869:CDS 35.0%
! CAATCTTGTCTGGTTTCTTT+AGG + chr6.1:9557743-9557762 None:intergenic 35.0%
! CATTTTCCCGACCAAATTTT+AGG + chr6.1:9558076-9558095 None:intergenic 35.0%
!! TCTTCTAGTTTCATGCTCTT+TGG - chr6.1:9556564-9556583 MS.gene83869:CDS 35.0%
ACTACAACGTCAATCAACCT+CGG - chr6.1:9557814-9557833 MS.gene83869:intron 40.0%
AGGAATGGAACACCTTTGTA+TGG - chr6.1:9558485-9558504 MS.gene83869:intron 40.0%
ATGCAGATGTGTAACCCTTA+TGG - chr6.1:9558362-9558381 MS.gene83869:intron 40.0%
ATTTCTCATTGTTCCACCGT+TGG + chr6.1:9557871-9557890 None:intergenic 40.0%
CCATTTCCACTACCATACAA+AGG + chr6.1:9558500-9558519 None:intergenic 40.0%
CTCAAATGTTGAATCTGCTG+CGG - chr6.1:9558019-9558038 MS.gene83869:intron 40.0%
GAAGTAGGATCAATGCCATA+AGG + chr6.1:9558380-9558399 None:intergenic 40.0%
GCTCTTTGGTAAGAAGATTC+CGG - chr6.1:9556578-9556597 MS.gene83869:CDS 40.0%
GGAAATGGAAATGTGTCCAT+AGG - chr6.1:9558512-9558531 MS.gene83869:intron 40.0%
GGAATTATCCATGAAGATGC+AGG + chr6.1:9558296-9558315 None:intergenic 40.0%
GGACATTGAACAATCTTGTC+TGG + chr6.1:9557753-9557772 None:intergenic 40.0%
GGTAATTCCATAAACCAGGA+GGG + chr6.1:9557289-9557308 None:intergenic 40.0%
TAGAAACCAACGACTCTTCT+AGG - chr6.1:9557985-9558004 MS.gene83869:intron 40.0%
TGAATCTGAGAAGAACTCAC+CGG + chr6.1:9556600-9556619 None:intergenic 40.0%
TGTCACAGATATTGGACCAA+CGG - chr6.1:9557852-9557871 MS.gene83869:intron 40.0%
TGTGTATCGTCTGTGACAAA+CGG - chr6.1:9558206-9558225 MS.gene83869:intron 40.0%
TTGATCCTACTTCAATGCAG+TGG - chr6.1:9558387-9558406 MS.gene83869:intron 40.0%
! AAAAGTGAACACAAGCACTG+TGG - chr6.1:9557677-9557696 MS.gene83869:intron 40.0%
! CATGTGTGTTTTCCAAGGAT+TGG + chr6.1:9558605-9558624 None:intergenic 40.0%
! GTGTCGAAACTTTTGCATCT+CGG + chr6.1:9557774-9557793 None:intergenic 40.0%
! TTGAATCTGCTGCGGTTTTT+AGG - chr6.1:9558027-9558046 MS.gene83869:intron 40.0%
!! AGAGTCGTTGGTTTCTAAAC+TGG + chr6.1:9557982-9558001 None:intergenic 40.0%
!! CCTTTGTATGGTAGTGGAAA+TGG - chr6.1:9558497-9558516 MS.gene83869:intron 40.0%
AACATAAGAGATAGCCCTCC+TGG - chr6.1:9557272-9557291 MS.gene83869:CDS 45.0%
ACCTTTGGATTCATCGAGAG+AGG + chr6.1:9558099-9558118 None:intergenic 45.0%
ATCAACCTCGGCATTTCTGT+CGG - chr6.1:9557826-9557845 MS.gene83869:intron 45.0%
ATGTTTGTAACCGCAACCCT+CGG + chr6.1:9558422-9558441 None:intergenic 45.0%
CACATGATTGTAGTGTCCGA+TGG - chr6.1:9557945-9557964 MS.gene83869:intron 45.0%
CATCGGACACTACAATCATG+TGG + chr6.1:9557947-9557966 None:intergenic 45.0%
GACTCTTCTAGGTACCAACA+TGG - chr6.1:9557996-9558015 MS.gene83869:intron 45.0%
GGAACACCTTTGTATGGTAG+TGG - chr6.1:9558491-9558510 MS.gene83869:intron 45.0%
GGGTAATTCCATAAACCAGG+AGG + chr6.1:9557290-9557309 None:intergenic 45.0%
GGTGAGTTCTTCTCAGATTC+AGG - chr6.1:9556599-9556618 MS.gene83869:CDS 45.0%
GTCGGAGTTGTCACAGATAT+TGG - chr6.1:9557844-9557863 MS.gene83869:intron 45.0%
GTTCCATTCCTGTTTGATGC+AGG + chr6.1:9558476-9558495 None:intergenic 45.0%
GTTCCTGCATCAAACAGGAA+TGG - chr6.1:9558470-9558489 MS.gene83869:intron 45.0%
TCAGGCCAATTCTACAACCA+TGG - chr6.1:9556617-9556636 MS.gene83869:CDS 45.0%
TGACACCACTGCATTGAAGT+AGG + chr6.1:9558395-9558414 None:intergenic 45.0%
TTGGTACCTAGAAGAGTCGT+TGG + chr6.1:9557994-9558013 None:intergenic 45.0%
TTGGTTTCTAAACTGGCGGT+CGG + chr6.1:9557975-9557994 None:intergenic 45.0%
!! GTCGTTGGTTTCTAAACTGG+CGG + chr6.1:9557979-9557998 None:intergenic 45.0%
!!! AAAAAAATGTTTTTTTTTTT+TGG - chr6.1:9556896-9556915 MS.gene83869:CDS 5.0%
AGAAATGTTGCTGTCGGTGC+AGG - chr6.1:9557885-9557904 MS.gene83869:intron 50.0%
AGAGCTACCCTGCATCTTCA+TGG - chr6.1:9558285-9558304 MS.gene83869:intron 50.0%
CACAGATATTGGACCAACGG+TGG - chr6.1:9557855-9557874 MS.gene83869:intron 50.0%
GAAATGTTGCTGTCGGTGCA+GGG - chr6.1:9557886-9557905 MS.gene83869:intron 50.0%
GAGATAGCCCTCCTGGTTTA+TGG - chr6.1:9557279-9557298 MS.gene83869:CDS 50.0%
GCCTCTCTCGATGAATCCAA+AGG - chr6.1:9558095-9558114 MS.gene83869:intron 50.0%
GTGACAAACGGTCATACTCG+AGG - chr6.1:9558218-9558237 MS.gene83869:intron 50.0%
! GGTAATGGCACACCAATCCT+TGG - chr6.1:9558590-9558609 MS.gene83869:CDS 50.0%
ACTGGCGGTCGGAATTCCAT+CGG + chr6.1:9557964-9557983 None:intergenic 55.0%
CAACTCCGACAGAAATGCCG+AGG + chr6.1:9557834-9557853 None:intergenic 55.0%
! GATGCAGGGTAGCTCTGCAT+AGG + chr6.1:9558281-9558300 None:intergenic 55.0%
TTGCTGTCGGTGCAGGGAGA+CGG - chr6.1:9557892-9557911 MS.gene83869:intron 60.0%
GTGTCACGCGCCGAGGGTTG+CGG - chr6.1:9558409-9558428 MS.gene83869:intron 70.0%
!! GCAGTGGTGTCACGCGCCGA+GGG - chr6.1:9558403-9558422 MS.gene83869:intron 70.0%
!! TGCAGTGGTGTCACGCGCCG+AGG - chr6.1:9558402-9558421 MS.gene83869:intron 70.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 9556531 9558625 9556531 ID=MS.gene83869
chr6.1 mRNA 9556531 9558625 9556531 ID=MS.gene83869.t1;Parent=MS.gene83869
chr6.1 exon 9558518 9558625 9558518 ID=MS.gene83869.t1.exon1;Parent=MS.gene83869.t1
chr6.1 CDS 9558518 9558625 9558518 ID=cds.MS.gene83869.t1;Parent=MS.gene83869.t1
chr6.1 exon 9556531 9557574 9556531 ID=MS.gene83869.t1.exon2;Parent=MS.gene83869.t1
chr6.1 CDS 9556531 9557574 9556531 ID=cds.MS.gene83869.t1;Parent=MS.gene83869.t1
Gene Sequence

>MS.gene83869

ATGAATGGTGAAGGTGACATGTTTGTTAAAGATTCTTCTAGTTTCATGCTCTTTGGTAAGAAGATTCCGGTGAGTTCTTCTCAGATTCAGGCCAATTCTACAACCATGGTAAATACTCATTTTATCATATTATATCATATACCCAATAACCATTGTTTATGTTGTTTTTTTATTTGAAAAAATTTAAGTATTTTTTATTTTTGTTGTATTGGGTTGGTATAATATTTTCCAATCTTTGAAACATTTAAATTTTTAAGAGTATTTGATCTATATGGTTTCTTTTTTAATATATTGATAAAAAAAACTCAAGAATATGGATCTGATTCATGTAAACAATGGCTGGTCTTAATTGTATGAATTCTAATAAAAAAATGTTTTTTTTTTTTGGAAATTTATAGTTGAGAATATATGTTTATGTCAATACATACATACATATATAGATGAATTTAAGAAACTGAGTTTAAGGTTTGTTAAGCTGTCTTATGAAAGTAGTTGGTTTTTTAAATAAAATTAGATCTGTTGCATTTTTTATGATACACTTATATTATTTTTGTCTGAAATTGAGTTGTACTTTTGAAAGAAATTTAACTTTAAATTTGTTTAATTTTGCTTGGAGTATTAATCAAATCTTAAAAACGTTCAATGTTTAATCAATTACAAATAGATTTGTTTTTCATAAATATAACATGATTGGTTGAATGAATGTGAAATAGACTGAAATTGTGGCAGATTAGTTTTAGTAACATAAGAGATAGCCCTCCTGGTTTATGGAATTACCCATAGTGAGTTTGACAAAATCACGGTGACATGTTTGTTGAAGCTCGAAAGAGTAGATTTTTCTAAATTACAGTGACACGTGATTATGTCAAACTCATCGTCAATCTAAACATACGAAATGAAAGTTCTCATGTTATGTACCACGTCGAATTTTATGGTTTTTTTATGTGAAAAATGAATATAACATTGATTTTGTTTCTTTTTTATTGTCAAATTTGTTGCCAAACTTTATTCTTCATGACTTATCACTGATTATATAGTTTTTATTTTACAGATTTCTTGCAGTAACTCAAAGCATAAAGGAAATGATATGCCTCATACTCAAAACATTGAGAAATCAAAGAATTCTTCTAATGAAGAAAATGAATCAAAAGTGAACACAAGCACTGTGGAAGATAATATGGAAACTAGCAGCACAGATCAAGACAAAGTCCTAAAGAAACCAGACAAGATTGTTCAATGTCCGAGATGCAAAAGTTTCGACACAAAATTTTGTTATTTCAACAACTACAACGTCAATCAACCTCGGCATTTCTGTCGGAGTTGTCACAGATATTGGACCAACGGTGGAACAATGAGAAATGTTGCTGTCGGTGCAGGGAGACGGAAGAACAAACACATAGCTTCTCAATATCGCCACATGATTGTAGTGTCCGATGGAATTCCGACCGCCAGTTTAGAAACCAACGACTCTTCTAGGTACCAACATGGCTCAAATGTTGAATCTGCTGCGGTTTTTAGGTGTTCAAATGATAATGGTATTGTCCTAAAATTTGGTCGGGAAAATGCCTCTCTCGATGAATCCAAAGGTTCAATGCTTAATCTTATGAACCACAAGAGAAATGTTGATGCAAGTGGTAACAATTGTAGAGAAAATGGAGAGGAAGAATCATCGGTATGTGTATCGTCTGTGACAAACGGTCATACTCGAGGAAACGAGTTATTCGAATCCGAACAGAATAGGTCTAAACCTATGCAGAGCTACCCTGCATCTTCATGGATAATTCCAATGAATCAAAGTTGGAACAATGTTACTTCTATGGTTCAATCATCAATGCAGATGTGTAACCCTTATGGCATTGATCCTACTTCAATGCAGTGGTGTCACGCGCCGAGGGTTGCGGTTACAAACATTTTCACACCAAACATTGGCTTGCAATTTGTTCCTGCATCAAACAGGAATGGAACACCTTTGTATGGTAGTGGAAATGGAAATGTGTCCATAGGTTCAAATGGTTGCATTTCACCTTCTTCATCTACTACTAGTAATAGTTGTATCTCAGGTAATGGCACACCAATCCTTGGAAAACACACATGA
Protein sequence

>MS.gene83869.t1

MNGEGDMFVKDSSSFMLFGKKIPVSSSQIQANSTTMISCSNSKHKGNDMPHTQNIEKSKNSSNEENESKVNTSTVEDNMETSSTDQDKVLKKPDKIVQCPRCKSFDTKFCYFNNYNVNQPRHFCRSCHRYWTNGGTMRNVAVGAGRRKNKHIASQYRHMIVVSDGIPTASLETNDSSRYQHGSNVESAAVFRCSNDNGIVLKFGRENASLDESKGSMLNLMNHKRNVDASGNNCRENGEEESSVCVSSVTNGHTRGNELFESEQNRSKPMQSYPASSWIIPMNQSWNNVTSMVQSSMQMCNPYGIDPTSMQWCHAPRVAVTNIFTPNIGLQFVPASNRNGTPLYGSGNGNVSIGSNGCISPSSSTTSNSCISGNGTPILGKHT