AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83872

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83872.t1 MTR_6g027390 85.667 300 5 2 1 262 1 300 0.0 511
MS.gene83872.t1 MTR_7g086940 50.530 283 108 8 1 262 1 272 1.18e-73 226
MS.gene83872.t1 MTR_2g015000 62.500 72 27 0 24 95 47 118 2.45e-27 109
MS.gene83872.t1 MTR_7g025010 62.857 70 26 0 24 93 39 108 4.82e-27 109
MS.gene83872.t1 MTR_4g088080 55.556 81 33 1 12 89 17 97 3.66e-23 96.7
MS.gene83872.t1 MTR_4g088070 61.905 63 24 0 30 92 27 89 4.53e-23 97.4
MS.gene83872.t1 MTR_8g107210 43.333 90 50 1 11 99 75 164 3.19e-17 80.5
MS.gene83872.t1 MTR_4g084550 36.264 91 50 2 7 96 45 128 8.79e-16 73.6
MS.gene83872.t1 MTR_1g115315 46.875 64 34 0 28 91 81 144 4.94e-14 70.5
MS.gene83872.t1 MTR_1g115315 46.875 64 34 0 28 91 81 144 9.90e-14 69.7
MS.gene83872.t1 MTR_3g115620 45.070 71 39 0 28 98 78 148 1.92e-13 67.8
MS.gene83872.t1 MTR_5g081990 34.524 84 55 0 12 95 13 96 2.49e-12 64.3
MS.gene83872.t1 MTR_4g063735 40.299 67 40 0 32 98 25 91 3.63e-12 65.1
MS.gene83872.t1 MTR_5g021930 40.625 64 37 1 31 93 29 92 5.25e-12 65.1
MS.gene83872.t1 MTR_7g060630 40.625 64 38 0 31 94 7 70 7.99e-12 63.2
MS.gene83872.t1 MTR_1g019130 42.188 64 37 0 31 94 89 152 4.44e-11 61.6
MS.gene83872.t1 MTR_7g023810 41.538 65 38 0 29 93 65 129 5.61e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83872.t1 AT5G17810 49.821 279 94 7 13 259 3 267 1.06e-72 223
MS.gene83872.t1 AT5G17810 49.821 279 94 7 13 259 3 267 1.06e-72 223
MS.gene83872.t1 AT5G17810 49.821 279 93 7 13 259 3 266 1.53e-72 223
MS.gene83872.t1 AT3G03660 54.122 279 93 6 3 259 2 267 1.74e-72 223
MS.gene83872.t1 AT3G03660 54.348 276 91 6 3 256 2 264 1.35e-70 219
MS.gene83872.t1 AT3G03660 49.026 308 93 8 3 259 2 296 9.41e-67 209
MS.gene83872.t1 AT3G03660 49.180 305 91 8 3 256 2 293 5.80e-65 206
MS.gene83872.t1 AT2G33880 62.162 74 28 0 23 96 46 119 1.40e-27 109
MS.gene83872.t1 AT2G33880 62.162 74 28 0 23 96 46 119 1.64e-27 109
MS.gene83872.t1 AT5G45980 57.534 73 31 0 30 102 53 125 1.02e-25 103
MS.gene83872.t1 AT4G35550 47.692 65 34 0 28 92 95 159 2.30e-14 71.6
MS.gene83872.t1 AT3G18010 40.000 80 47 1 29 107 73 152 4.84e-14 71.2
MS.gene83872.t1 AT5G59340 39.706 68 41 0 31 98 13 80 5.48e-13 67.4
MS.gene83872.t1 AT1G20700 42.254 71 41 0 28 98 29 99 1.21e-12 64.3
MS.gene83872.t1 AT1G20700 42.254 71 41 0 28 98 91 161 2.54e-12 64.7
MS.gene83872.t1 AT1G20700 42.254 71 41 0 28 98 91 161 3.04e-12 64.3
MS.gene83872.t1 AT2G28610 38.889 72 44 0 26 97 2 73 3.05e-12 65.1
MS.gene83872.t1 AT2G17950 42.188 64 36 1 31 93 37 100 4.11e-12 65.5
MS.gene83872.t1 AT3G11260 39.062 64 39 0 32 95 24 87 6.97e-12 63.2
MS.gene83872.t1 AT1G46480 42.188 64 37 0 31 94 89 152 1.19e-11 62.4
MS.gene83872.t1 AT5G05770 37.500 64 40 0 32 95 29 92 5.12e-11 59.3
MS.gene83872.t1 AT1G46480 42.188 64 37 0 31 94 89 152 5.61e-11 61.6

Find 48 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAACTTCTTGAGAAATT+TGG 0.100600 6.1:-9468238 MS.gene83872:CDS
ATTCTCACGGTTTCATCTTT+TGG 0.207436 6.1:+9468262 None:intergenic
GACCATTCCACTATTGAAAA+TGG 0.216187 6.1:+9468291 None:intergenic
ATAAGGATTTGTTCAGGTTT+TGG 0.291953 6.1:+9468322 None:intergenic
TGAAAATGGATTCAAGTATA+AGG 0.302638 6.1:+9468305 None:intergenic
CTTGGTGCTGCACTTGGTCT+TGG 0.327659 6.1:-9468055 MS.gene83872:CDS
CTGCACTGGTATTGGTGATA+TGG 0.337777 6.1:+9468100 None:intergenic
CTGTCTCTTGGTGCTGCACT+TGG 0.345570 6.1:-9468061 MS.gene83872:CDS
GTAATATTGGTTCATTCCTC+TGG 0.359362 6.1:-9466164 MS.gene83872:CDS
GTTTGGTGAAGATGTAATAT+TGG 0.373946 6.1:-9466177 MS.gene83872:CDS
TCAAATCAATTTGCCTCAAA+TGG 0.378394 6.1:-9467840 MS.gene83872:CDS
TCGGTTCCAACCAATGAGTT+TGG 0.380496 6.1:-9466137 MS.gene83872:CDS
TGTTGGTGCTGCACTGGTAT+TGG 0.396373 6.1:+9468092 None:intergenic
TCAAGTATAAGGATTTGTTC+AGG 0.405750 6.1:+9468316 None:intergenic
CAGTACTGCCACCGCTTAGT+TGG 0.406811 6.1:+9468128 None:intergenic
GTACCTCTTGGCATTTCTGT+TGG 0.409908 6.1:+9466221 None:intergenic
AGTACTGCCACCGCTTAGTT+GGG 0.417375 6.1:+9468129 None:intergenic
GCATGGTGAAAGCTACTATC+TGG 0.420994 6.1:-9466093 MS.gene83872:intron
GCTGAAGTGTTGTCCATTTG+AGG 0.434913 6.1:+9467827 None:intergenic
CTTGAGAAATTTGGAGCTGT+TGG 0.435040 6.1:-9468229 MS.gene83872:CDS
GGTGCTGCACTTGGTCTTGG+TGG 0.436302 6.1:-9468052 MS.gene83872:CDS
ATTGACATGAAAACAATGTT+TGG 0.438397 6.1:-9466194 MS.gene83872:CDS
GTTGTTTGGATTATTAGTAT+TGG 0.439777 6.1:+9468381 None:intergenic
GGTTCATTCCTCTGGAGTCT+CGG 0.469528 6.1:-9466156 MS.gene83872:CDS
TCAAATTTCAACTATTACCC+AGG 0.481334 6.1:-9467770 MS.gene83872:intron
AACAGTACCTCTCTGTCTCT+TGG 0.490108 6.1:-9468073 MS.gene83872:CDS
CATCAAGAAACCAAACTCAT+TGG 0.493495 6.1:+9466127 None:intergenic
AAGAAACCAAACTCATTGGT+TGG 0.499044 6.1:+9466131 None:intergenic
GTGATCACAGTGTTTATCAA+TGG 0.505244 6.1:-9466248 MS.gene83872:CDS
ATGTCAATTGCAGTACCTCT+TGG 0.508431 6.1:+9466209 None:intergenic
AGGTACTGTTGGTGCTGCAC+TGG 0.511056 6.1:+9468086 None:intergenic
CGGCCTGCATCTGCCTCTGA+CGG 0.528621 6.1:+9468155 None:intergenic
ACGCCGTCAGAGGCAGATGC+AGG 0.544113 6.1:-9468158 MS.gene83872:CDS
AAATTTGAAGCATGATCAGA+AGG 0.576369 6.1:+9467785 None:intergenic
GCCGCCGCCCAACTAAGCGG+TGG 0.588442 6.1:-9468136 MS.gene83872:CDS
GTGAAACTGTGAGATCAAGA+TGG 0.590101 6.1:-9468348 MS.gene83872:CDS
CTCGCTCTAGACGCCGTCAG+AGG 0.591369 6.1:-9468168 MS.gene83872:CDS
ACTGCCACCGCTTAGTTGGG+CGG 0.608647 6.1:+9468132 None:intergenic
AGAATTCATAGTGAAAGTTG+AGG 0.624134 6.1:+9468027 None:intergenic
GCCACCGCTTAGTTGGGCGG+CGG 0.641411 6.1:+9468135 None:intergenic
CAGGCCGCCGCCCAACTAAG+CGG 0.643678 6.1:-9468139 MS.gene83872:CDS
TGCAGCACCAAGAGACAGAG+AGG 0.645908 6.1:+9468066 None:intergenic
AACTTTGAGACTGAACATGT+TGG 0.650519 6.1:+9468404 None:intergenic
AAGAGACAGAGAGGTACTGT+TGG 0.665242 6.1:+9468075 None:intergenic
TTGATGCAGAACTTGCAGCA+TGG 0.678300 6.1:-9466110 MS.gene83872:CDS
GACGGCGTCTAGAGCGAGAG+CGG 0.680232 6.1:+9468173 None:intergenic
GGTTGGAACCGAGACTCCAG+AGG 0.685747 6.1:+9466148 None:intergenic
GTTCCAACAGAAATGCCAAG+AGG 0.748646 6.1:-9466224 MS.gene83872:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTATTTATATTAATTTTA+GGG - chr6.1:9467909-9467928 MS.gene83872:intron 0.0%
!!! TATTTATTTATATTAATTTT+AGG - chr6.1:9467908-9467927 MS.gene83872:intron 0.0%
!! ACTTTCAAAAAAAAAAAAAA+TGG + chr6.1:9466652-9466671 None:intergenic 10.0%
!! TGTTCATAAAAAAAAATTAT+AGG + chr6.1:9466504-9466523 None:intergenic 10.0%
!!! AAATAAAACTATTCTTTTTT+TGG - chr6.1:9466971-9466990 MS.gene83872:intron 10.0%
!!! AATATCATATTTTTTTGAAA+AGG + chr6.1:9466709-9466728 None:intergenic 10.0%
!!! ATATCATATTTTTTTGAAAA+GGG + chr6.1:9466708-9466727 None:intergenic 10.0%
!!! GTTTAGAAATTTTTAAATTA+AGG + chr6.1:9467199-9467218 None:intergenic 10.0%
!!! TACTTATAATAATAACTTTT+CGG - chr6.1:9466220-9466239 MS.gene83872:CDS 10.0%
!!! TTATTTATATTAATTTTAGG+GGG - chr6.1:9467911-9467930 MS.gene83872:intron 10.0%
!!! TTATTTCAAAAACTTTATTT+AGG + chr6.1:9468380-9468399 None:intergenic 10.0%
!! GAAAAATAAGTTAAAAAAGA+AGG + chr6.1:9467381-9467400 None:intergenic 15.0%
!!! ACTTATAATAATAACTTTTC+GGG - chr6.1:9466221-9466240 MS.gene83872:CDS 15.0%
!!! AGTTTTTGAAATAAATGTTT+TGG - chr6.1:9468386-9468405 MS.gene83872:CDS 15.0%
!!! CATTTTTTTAAACTAATCAA+TGG + chr6.1:9467276-9467295 None:intergenic 15.0%
!!! TATCATATTTTTTTGAAAAG+GGG + chr6.1:9466707-9466726 None:intergenic 15.0%
!!! TTAAATTAAGGAATTAGTTT+TGG + chr6.1:9467187-9467206 None:intergenic 15.0%
!! GAAAAGAGAAAAATAATCTA+GGG + chr6.1:9467221-9467240 None:intergenic 20.0%
!! GAATAGCAATTTCTTTAATT+TGG + chr6.1:9467359-9467378 None:intergenic 20.0%
!! GATTGAAGAATTAATAAAAG+TGG - chr6.1:9467015-9467034 MS.gene83872:intron 20.0%
!! GTTAAGATGATTACAAAATT+TGG + chr6.1:9466679-9466698 None:intergenic 20.0%
!! TGAAAAGAGAAAAATAATCT+AGG + chr6.1:9467222-9467241 None:intergenic 20.0%
!! TTTCTCACAATATTAATTGT+AGG + chr6.1:9467735-9467754 None:intergenic 20.0%
!!! ACTAATTTTTGTCCTTAAAA+AGG + chr6.1:9466757-9466776 None:intergenic 20.0%
!!! ATCATATTTTTTTGAAAAGG+GGG + chr6.1:9466706-9466725 None:intergenic 20.0%
!!! CTAATTTTTGTCCTTAAAAA+GGG + chr6.1:9466756-9466775 None:intergenic 20.0%
!!! TATGTCATATGTAATAGTTT+TGG - chr6.1:9467856-9467875 MS.gene83872:intron 20.0%
!!! TTCTTTAATTTGGTATAATG+TGG + chr6.1:9467349-9467368 None:intergenic 20.0%
! AATAAATTAAGCACATACCT+GGG + chr6.1:9466463-9466482 None:intergenic 25.0%
! ACCTCTCAAAAATAGTTTAA+AGG - chr6.1:9468260-9468279 MS.gene83872:CDS 25.0%
! AGAAAACTTCTTGAGAAATT+TGG - chr6.1:9465975-9465994 MS.gene83872:intron 25.0%
! AGAAAGGAATGTGATAAAAA+GGG + chr6.1:9467155-9467174 None:intergenic 25.0%
! ATGTGCTTAATTTATTGCAT+GGG - chr6.1:9466467-9466486 MS.gene83872:intron 25.0%
! ATTGACATGAAAACAATGTT+TGG - chr6.1:9468019-9468038 MS.gene83872:CDS 25.0%
! CTGAAACCAATAAAAAACAT+TGG + chr6.1:9466015-9466034 None:intergenic 25.0%
! GTTGTTTGGATTATTAGTAT+TGG + chr6.1:9465835-9465854 None:intergenic 25.0%
! TAATTGTAGGTTTCAATTGT+CGG + chr6.1:9467722-9467741 None:intergenic 25.0%
! TATGTGCTTAATTTATTGCA+TGG - chr6.1:9466466-9466485 MS.gene83872:intron 25.0%
!! AAGAAGTTTTCTTATTCTCA+CGG + chr6.1:9465967-9465986 None:intergenic 25.0%
!! AGTTTTCAACAAAATTGTCA+TGG - chr6.1:9467672-9467691 MS.gene83872:intron 25.0%
!! TGAAAATGGATTCAAGTATA+AGG + chr6.1:9465911-9465930 None:intergenic 25.0%
!! TTAAGACAAAACCCTTTTTA+AGG - chr6.1:9466742-9466761 MS.gene83872:intron 25.0%
!! TTTTTATCACATTCCTTTCT+GGG - chr6.1:9467155-9467174 MS.gene83872:intron 25.0%
!!! ACCTTTAAACTATTTTTGAG+AGG + chr6.1:9468264-9468283 None:intergenic 25.0%
!!! ATATTAATTTTAGGGGGAAT+AGG - chr6.1:9467917-9467936 MS.gene83872:intron 25.0%
!!! ATTTTGTTATACTCTTCTCT+AGG - chr6.1:9467250-9467269 MS.gene83872:intron 25.0%
!!! TGGTTTATAATCAGTTTTGA+TGG - chr6.1:9466991-9467010 MS.gene83872:intron 25.0%
!!! TTTTTTCTGGAATGAATGAA+GGG + chr6.1:9467463-9467482 None:intergenic 25.0%
AGAATTCATAGTGAAAGTTG+AGG + chr6.1:9466189-9466208 None:intergenic 30.0%
CAATAAATTAAGCACATACC+TGG + chr6.1:9466464-9466483 None:intergenic 30.0%
CAGAAAGGAATGTGATAAAA+AGG + chr6.1:9467156-9467175 None:intergenic 30.0%
GACGACACTTATAAATGAAA+CGG + chr6.1:9468174-9468193 None:intergenic 30.0%
TCAAATCAATTTGCCTCAAA+TGG - chr6.1:9466373-9466392 MS.gene83872:intron 30.0%
TCAAATTTCAACTATTACCC+AGG - chr6.1:9466443-9466462 MS.gene83872:intron 30.0%
TGTAAGAAACCTGTTTAAGT+TGG + chr6.1:9466606-9466625 None:intergenic 30.0%
! AAAGTGAACTCATGTTTTTC+CGG + chr6.1:9467111-9467130 None:intergenic 30.0%
! AAATTTGAAGCATGATCAGA+AGG + chr6.1:9466431-9466450 None:intergenic 30.0%
! AATCGATGATAATGCAAATG+TGG - chr6.1:9467558-9467577 MS.gene83872:intron 30.0%
! CTTGAATCCATTTTCAATAG+TGG - chr6.1:9465915-9465934 MS.gene83872:intron 30.0%
! CTTTTTATCACATTCCTTTC+TGG - chr6.1:9467154-9467173 MS.gene83872:intron 30.0%
! GTAAGAAACCTGTTTAAGTT+GGG + chr6.1:9466605-9466624 None:intergenic 30.0%
! GTTGTACTAATCTTCTTTTC+CGG - chr6.1:9467089-9467108 MS.gene83872:intron 30.0%
! GTTTGGTGAAGATGTAATAT+TGG - chr6.1:9468036-9468055 MS.gene83872:CDS 30.0%
! TATGCTTTTCATTTAATCCC+AGG + chr6.1:9467066-9467085 None:intergenic 30.0%
! TCAAGTATAAGGATTTGTTC+AGG + chr6.1:9465900-9465919 None:intergenic 30.0%
!! ATAAGGATTTGTTCAGGTTT+TGG + chr6.1:9465894-9465913 None:intergenic 30.0%
!! ATCCATTTTCAATAGTGGAA+TGG - chr6.1:9465920-9465939 MS.gene83872:intron 30.0%
!! ATTTTGATCCCAACTTAAAC+AGG - chr6.1:9466594-9466613 MS.gene83872:intron 30.0%
!!! CTTTTTTCTGGAATGAATGA+AGG + chr6.1:9467464-9467483 None:intergenic 30.0%
!!! GTGATGCCAATGTTTTTTAT+TGG - chr6.1:9466006-9466025 MS.gene83872:intron 30.0%
!!! TTTTTCTGGAATGAATGAAG+GGG + chr6.1:9467462-9467481 None:intergenic 30.0%
AAGAAACCAAACTCATTGGT+TGG + chr6.1:9468085-9468104 None:intergenic 35.0%
CATCAAGAAACCAAACTCAT+TGG + chr6.1:9468089-9468108 None:intergenic 35.0%
GAATCTACAACACTTGTCAA+AGG + chr6.1:9466923-9466942 None:intergenic 35.0%
GACCATTCCACTATTGAAAA+TGG + chr6.1:9465925-9465944 None:intergenic 35.0%
GGAACTGTCAAATCAACAAA+AGG + chr6.1:9467045-9467064 None:intergenic 35.0%
GTGATCACAGTGTTTATCAA+TGG - chr6.1:9467965-9467984 MS.gene83872:intron 35.0%
GTTTCAATTGTCGGTGATAT+TGG + chr6.1:9467713-9467732 None:intergenic 35.0%
! ATTCTCACGGTTTCATCTTT+TGG + chr6.1:9465954-9465973 None:intergenic 35.0%
!! AACTTTGAGACTGAACATGT+TGG + chr6.1:9465812-9465831 None:intergenic 35.0%
!! ATTTTAGGGGGAATAGGATA+TGG - chr6.1:9467923-9467942 MS.gene83872:intron 35.0%
!! GTAATATTGGTTCATTCCTC+TGG - chr6.1:9468049-9468068 MS.gene83872:CDS 35.0%
!! TCACTCTTTTCAGTGTTGTT+TGG + chr6.1:9465849-9465868 None:intergenic 35.0%
!! TTGTTGATTTGACAGTTCCT+GGG - chr6.1:9467046-9467065 MS.gene83872:intron 35.0%
!! TTTGTTGATTTGACAGTTCC+TGG - chr6.1:9467045-9467064 MS.gene83872:intron 35.0%
!! TTTTAGGGGGAATAGGATAT+GGG - chr6.1:9467924-9467943 MS.gene83872:intron 35.0%
!!! CACTGTGATCTACTTTTTTC+TGG + chr6.1:9467476-9467495 None:intergenic 35.0%
!!! TGTTTTGGTGTTTTTGTTCC+AGG - chr6.1:9468401-9468420 MS.gene83872:CDS 35.0%
ATGTCAATTGCAGTACCTCT+TGG + chr6.1:9468007-9468026 None:intergenic 40.0%
CCTTGGGAAGACAAAATGAA+GGG + chr6.1:9467420-9467439 None:intergenic 40.0%
GTGAAACTGTGAGATCAAGA+TGG - chr6.1:9465865-9465884 MS.gene83872:intron 40.0%
TTTAGGGGGAATAGGATATG+GGG - chr6.1:9467925-9467944 MS.gene83872:intron 40.0%
! CTTGAGAAATTTGGAGCTGT+TGG - chr6.1:9465984-9466003 MS.gene83872:intron 40.0%
!! GTTTTGGATGAAACCCAGAA+AGG + chr6.1:9467171-9467190 None:intergenic 40.0%
AACAGTACCTCTCTGTCTCT+TGG - chr6.1:9466140-9466159 MS.gene83872:CDS 45.0%
CCTTTGTCACACTTTGCCTT+GGG + chr6.1:9467436-9467455 None:intergenic 45.0%
GCATGGTGAAAGCTACTATC+TGG - chr6.1:9468120-9468139 MS.gene83872:CDS 45.0%
GCCTTGGGAAGACAAAATGA+AGG + chr6.1:9467421-9467440 None:intergenic 45.0%
GGTTCGTCTTCATCATGTGT+TGG - chr6.1:9466242-9466261 MS.gene83872:CDS 45.0%
GTACCTCTTGGCATTTCTGT+TGG + chr6.1:9467995-9468014 None:intergenic 45.0%
GTTCCAACAGAAATGCCAAG+AGG - chr6.1:9467989-9468008 MS.gene83872:intron 45.0%
TATGGGGGGAGTAATGTTTC+AGG - chr6.1:9467941-9467960 MS.gene83872:intron 45.0%
TCCTTTGTCACACTTTGCCT+TGG + chr6.1:9467437-9467456 None:intergenic 45.0%
TCGGTTCCAACCAATGAGTT+TGG - chr6.1:9468076-9468095 MS.gene83872:CDS 45.0%
TCGTCTTCATCATGTGTTGG+TGG - chr6.1:9466245-9466264 MS.gene83872:CDS 45.0%
TTAGGGGGAATAGGATATGG+GGG - chr6.1:9467926-9467945 MS.gene83872:intron 45.0%
TTGATGCAGAACTTGCAGCA+TGG - chr6.1:9468103-9468122 MS.gene83872:CDS 45.0%
! CCCTTCATTTTGTCTTCCCA+AGG - chr6.1:9467417-9467436 MS.gene83872:intron 45.0%
! CTCACGGTTTCATCTTTTGG+TGG + chr6.1:9465951-9465970 None:intergenic 45.0%
!! AAGAGACAGAGAGGTACTGT+TGG + chr6.1:9466141-9466160 None:intergenic 45.0%
!! CTGCACTGGTATTGGTGATA+TGG + chr6.1:9466116-9466135 None:intergenic 45.0%
!! GCTGAAGTGTTGTCCATTTG+AGG + chr6.1:9466389-9466408 None:intergenic 45.0%
!! GGGTTTGGTGGTTTGTACTT+AGG - chr6.1:9466344-9466363 MS.gene83872:intron 45.0%
!! ATTAAACAAAAATTATTTAA+AGG + chr6.1:9466844-9466863 None:intergenic 5.0%
!! TGAAATAAAAAAATTTATTT+AGG + chr6.1:9468349-9468368 None:intergenic 5.0%
!! TTTAAAAAATATATAAAAGT+AGG + chr6.1:9466953-9466972 None:intergenic 5.0%
!!! TTTATTTATATTAATTTTAG+GGG - chr6.1:9467910-9467929 MS.gene83872:intron 5.0%
AGTACTGCCACCGCTTAGTT+GGG + chr6.1:9466087-9466106 None:intergenic 50.0%
CCCAAGGCAAAGTGTGACAA+AGG - chr6.1:9467433-9467452 MS.gene83872:intron 50.0%
CTTGTGCTTGCTTTGACACC+TGG + chr6.1:9468422-9468441 None:intergenic 50.0%
TAGGGGGAATAGGATATGGG+GGG - chr6.1:9467927-9467946 MS.gene83872:intron 50.0%
!! GGTTCATTCCTCTGGAGTCT+CGG - chr6.1:9468057-9468076 MS.gene83872:CDS 50.0%
!! TCATCATGTGTTGGTGGTGC+TGG - chr6.1:9466251-9466270 MS.gene83872:CDS 50.0%
!! TGTTGGTGCTGCACTGGTAT+TGG + chr6.1:9466124-9466143 None:intergenic 50.0%
CAGTACTGCCACCGCTTAGT+TGG + chr6.1:9466088-9466107 None:intergenic 55.0%
CTGCTGCTGGTGGAGAACAA+GGG - chr6.1:9466324-9466343 MS.gene83872:intron 55.0%
GCTGGTGGAGAACAAGGGTT+TGG - chr6.1:9466329-9466348 MS.gene83872:intron 55.0%
GGTGGAGAACAAGGGTTTGG+TGG - chr6.1:9466332-9466351 MS.gene83872:intron 55.0%
TGCAGCACCAAGAGACAGAG+AGG + chr6.1:9466150-9466169 None:intergenic 55.0%
!! AGGTACTGTTGGTGCTGCAC+TGG + chr6.1:9466130-9466149 None:intergenic 55.0%
!! CTGTCTCTTGGTGCTGCACT+TGG - chr6.1:9466152-9466171 MS.gene83872:CDS 55.0%
!! CTTGGTGCTGCACTTGGTCT+TGG - chr6.1:9466158-9466177 MS.gene83872:CDS 55.0%
!! TGTGTTGGTGGTGCTGGAGT+TGG - chr6.1:9466257-9466276 MS.gene83872:CDS 55.0%
ACTGCCACCGCTTAGTTGGG+CGG + chr6.1:9466084-9466103 None:intergenic 60.0%
GCTGCTGCTGGTGGAGAACA+AGG - chr6.1:9466323-9466342 MS.gene83872:intron 60.0%
GGTTGGAACCGAGACTCCAG+AGG + chr6.1:9468068-9468087 None:intergenic 60.0%
!! CTTGGTCTTGGTGCTGCTGC+TGG - chr6.1:9466311-9466330 MS.gene83872:intron 60.0%
!! GCTGGAGTTGGTGTTGGTGC+TGG - chr6.1:9466269-9466288 MS.gene83872:CDS 60.0%
!! GCTGGTGCTGGTCTTGGTCT+TGG - chr6.1:9466299-9466318 MS.gene83872:intron 60.0%
!! GGTGCTGCACTTGGTCTTGG+TGG - chr6.1:9466161-9466180 MS.gene83872:CDS 60.0%
!! GGTGGTGCTGGAGTTGGTGT+TGG - chr6.1:9466263-9466282 MS.gene83872:CDS 60.0%
!! GTTGGTGTTGGTGCTGGTGC+TGG - chr6.1:9466275-9466294 MS.gene83872:CDS 60.0%
ACGCCGTCAGAGGCAGATGC+AGG - chr6.1:9466055-9466074 MS.gene83872:intron 65.0%
CGGCCTGCATCTGCCTCTGA+CGG + chr6.1:9466061-9466080 None:intergenic 65.0%
! CTCGCTCTAGACGCCGTCAG+AGG - chr6.1:9466045-9466064 MS.gene83872:intron 65.0%
! GACGGCGTCTAGAGCGAGAG+CGG + chr6.1:9466043-9466062 None:intergenic 65.0%
!! GCTGGTGCTGGTGCTGGTCT+TGG - chr6.1:9466293-9466312 MS.gene83872:intron 65.0%
!! GGTCTTGGTGCTGCTGCTGG+TGG - chr6.1:9466314-9466333 MS.gene83872:intron 65.0%
!! GTTGGTGCTGGTGCTGGTGC+TGG - chr6.1:9466281-9466300 MS.gene83872:CDS 65.0%
CAGGCCGCCGCCCAACTAAG+CGG - chr6.1:9466074-9466093 MS.gene83872:intron 70.0%
GCCACCGCTTAGTTGGGCGG+CGG + chr6.1:9466081-9466100 None:intergenic 70.0%
!! GCTGGTGCTGGTGCTGGTGC+TGG - chr6.1:9466287-9466306 MS.gene83872:intron 70.0%
GCCGCCGCCCAACTAAGCGG+TGG - chr6.1:9466077-9466096 MS.gene83872:intron 75.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 9465789 9468446 9465789 ID=MS.gene83872
chr6.1 mRNA 9465789 9468446 9465789 ID=MS.gene83872.t1;Parent=MS.gene83872
chr6.1 exon 9467993 9468446 9467993 ID=MS.gene83872.t1.exon1;Parent=MS.gene83872.t1
chr6.1 CDS 9467993 9468446 9467993 ID=cds.MS.gene83872.t1;Parent=MS.gene83872.t1
chr6.1 exon 9467771 9467869 9467771 ID=MS.gene83872.t1.exon2;Parent=MS.gene83872.t1
chr6.1 CDS 9467771 9467869 9467771 ID=cds.MS.gene83872.t1;Parent=MS.gene83872.t1
chr6.1 exon 9466094 9466305 9466094 ID=MS.gene83872.t1.exon3;Parent=MS.gene83872.t1
chr6.1 CDS 9466094 9466305 9466094 ID=cds.MS.gene83872.t1;Parent=MS.gene83872.t1
chr6.1 exon 9465789 9465812 9465789 ID=MS.gene83872.t1.exon4;Parent=MS.gene83872.t1
chr6.1 CDS 9465789 9465812 9465789 ID=cds.MS.gene83872.t1;Parent=MS.gene83872.t1
Gene Sequence

>MS.gene83872

ATGGAAGATCAACAACAAAACCAACATGTTCAGTCTCAAAGTTCCAATACTAATAATCCAAACAACACTGAAAAGAGTGAAACTGTGAGATCAAGATGGACACCAAAACCTGAACAAATCCTTATACTTGAATCCATTTTCAATAGTGGAATGGTCAATCCACCAAAAGATGAAACCGTGAGAATAAGAAAACTTCTTGAGAAATTTGGAGCTGTTGGTGATGCCAATGTTTTTTATTGGTTTCAGAATCGCCGCTCTCGCTCTAGACGCCGTCAGAGGCAGATGCAGGCCGCCGCCCAACTAAGCGGTGGCAGTACTGCAAATCCATATCACCAATACCAGTGCAGCACCAACAGTACCTCTCTGTCTCTTGGTGCTGCACTTGGTCTTGGTGGTTCCTCAACTTTCACTATGAATTCTTCTTCTTGTTCTACTTATAATAATAACTTTTCGGGTTCGTCTTCATCATGTGTTGGTGGTGCTGGAGTTGGTGTTGGTGCTGGTGCTGGTGCTGGTGCTGGTCTTGGTCTTGGTGCTGCTGCTGGTGGAGAACAAGGGTTTGGTGGTTTGTACTTAGGCTCTACTCAAATCAATTTGCCTCAAATGGACAACACTTCAGCTGCTTCATCAATTTTGCAGCCTTCTGATCATGCTTCAAATTTCAACTATTACCCAGGTATGTGCTTAATTTATTGCATGGGTGTGTTATAATCCTATAATTTTTTTTTATGAACAAAAAAACTAGATTTCAAATTAAATTGAGTAAAGAAATACTCAACTCTTCATATGAATTCTATTTAATTTTATTTTGATCCCAACTTAAACAGGTTTCTTACATGATTCATTTGTTAAATCTTCTTCCATTTTTTTTTTTTTTGAAAGTTCTTCCAAATTTTGTAATCATCTTAACAAAACCCCCTTTTCAAAAAAATATGATATTTTTCAGATTCTCTTTAAGACAAAACCCTTTTTAAGGACAAAAATTAGTACTAGAATCTATATTACTTTTCTAAACGTAAATTAATTTAATACTTCTGTCATGTTATTAGTCTCCTTTAAATAATTTTTGTTTAATTAGTTTAGACTGATTATTTTAATAAACTCTAAAACAAGTTAGAACTTTCATTAAATCCTTTGACAAGTGTTGTAGATTCAGATTCACCTACTTTTATATATTTTTTAAAATAAAACTATTCTTTTTTTGGTTTATAATCAGTTTTGATGGTGATTGAAGAATTAATAAAAGTGGAAAACCTTTTGTTGATTTGACAGTTCCTGGGATTAAATGAAAAGCATAAAAGTTGTACTAATCTTCTTTTCCGGAAAAACATGAGTTCACTTTCTTGTTTCAGTTTCTGTTCTTCCCTTTTTATCACATTCCTTTCTGGGTTTCATCCAAAACTAATTCCTTAATTTAAAAATTTCTAAACCCTAGATTATTTTTCTCTTTTCATTTTACTAATTTTGTTATACTCTTCTCTAGGCCATTGATTAGTTTAAAAAAATGATTTAGATAATTTACATATTGTCATCATAAATTGAGAAACATTATATTGTCCACATTATACCAAATTAAAGAAATTGCTATTCCTTCTTTTTTAACTTATTTTTCTATATGATATTCTGTGCCCTTCATTTTGTCTTCCCAAGGCAAAGTGTGACAAAGGAAACCCCTTCATTCATTCCAGAAAAAAGTAGATCACAGTGCAGCAAATTAATCTAATACACTCATAATACTAAAATGTACAAGCTTTCAAATTACACTCACAAATCGATGATAATGCAAATGTGGCGATGATGTAATGATTGATTAATGTATGTTTGAATTGACTAGTTTGACAAAATCACACTGACGTCATGATTTTGTTGAAGCTACAGAGTGTAGTTTTCAACAAAATTGTCATGGCTTACGTTCATTCTGCCAATATCACCGACAATTGAAACCTACAATTAATATTGTGAGAAAAAGACGATAATAAACTAAATTTGTTCTTGCATTATTTTTATATAATTATTTTTATAATGAAGTTTTTTTTTTTTAATAATGAAGTTTTATATTTTGTTTTGTATGTCATATGTAATAGTTTTGGTGTGTTTATAATTGATTGATATGCTTTAATATTTATTTATATTAATTTTAGGGGGAATAGGATATGGGGGGAGTAATGTTTCAGGTGTGATCACAGTGTTTATCAATGGAGTTCCAACAGAAATGCCAAGAGGTACTGCAATTGACATGAAAACAATGTTTGGTGAAGATGTAATATTGGTTCATTCCTCTGGAGTCTCGGTTCCAACCAATGAGTTTGGTTTCTTGATGCAGAACTTGCAGCATGGTGAAAGCTACTATCTGGTAATTTAGCACTCATTCAACATACTTTTCCGTTTCATTTATAAGTGTCGTCTTTAAAATGCTTTTTCGACTCAAATTATTTATCACTCTTTTTATAAACATAGTGCGTCTGATATGAACCTCTCAAAAATAGTTTAAAGGTTGACATTTATTATTTTTAATAAAAAATAAATTTTATAATTAGAAATATTTTCTTTGTGTACACCTAAATAAATTTTTTTATTTCAAAAAAAAACCTAAATAAAGTTTTTGAAATAAATGTTTTGGTGTTTTTGTTCCAGGTGTCAAAGCAAGCACAAGCTTGA
Protein sequence

>MS.gene83872.t1

MEDQQQNQHVQSQSSNTNNPNNTEKSETVRSRWTPKPEQILILESIFNSGMVNPPKDETVRIRKLLEKFGAVGDANVFYWFQNRRSRSRRRQRQMQAAAQLSGGSTANPYHQYQCSTNSTSLSLGAALGLGGSSTFTMNSSSCSTYNNNFSGSTQINLPQMDNTSAASSILQPSDHASNFNYYPGGIGYGGSNVSGVITVFINGVPTEMPRGTAIDMKTMFGEDVILVHSSGVSVPTNEFGFLMQNLQHGESYYLVSKQAQA