AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84048

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84048.t1 MTR_1g035640 61.625 443 55 6 2 395 89 465 1.32e-167 477
MS.gene84048.t1 MTR_1g035490 34.551 356 153 10 42 395 52 329 7.34e-52 176
MS.gene84048.t1 MTR_1g035620 31.750 400 181 16 2 393 93 408 9.21e-36 135
MS.gene84048.t1 MTR_1g035570 41.011 178 76 3 2 161 89 255 3.46e-30 120
MS.gene84048.t1 MTR_1g035570 72.368 76 21 0 320 395 320 395 5.83e-29 117
MS.gene84048.t1 MTR_1g050845 38.627 233 108 10 2 222 93 302 1.59e-26 112
MS.gene84048.t1 MTR_6g061070 28.337 427 215 17 2 394 101 470 9.26e-24 102
MS.gene84048.t1 MTR_1g035650 43.103 116 57 2 2 113 93 203 1.27e-15 75.5
MS.gene84048.t1 MTR_1g035520 49.180 61 31 0 334 394 323 383 2.90e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 76 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAGGTTGGATAAAATTTA+TGG 0.171908 1.3:-32698477 MS.gene84048:CDS
TATTTCAAGGTTAAGATATT+TGG 0.184615 1.3:-32702251 MS.gene84048:intron
AAAGAAGGGATTCTCATTAT+TGG 0.209323 1.3:+32701820 None:intergenic
CCATGGGAAGCATGCTCTTT+TGG 0.216932 1.3:+32700173 None:intergenic
CCTTGAAGAGCTTGCACTTT+TGG 0.221116 1.3:+32700861 None:intergenic
CCCTGAAGAGCATGCACTTT+TGG 0.222036 1.3:+32700671 None:intergenic
TCCAAAAGTGCATGCTCTTC+AGG 0.235087 1.3:-32700672 MS.gene84048:CDS
CCCTGGAGAGCATGCACTTT+TGG 0.251459 1.3:+32700480 None:intergenic
GCATGCACCAAAGAGATTTA+TGG 0.255225 1.3:-32698600 MS.gene84048:CDS
CCTCTTTCCATAAATCTCTT+TGG 0.284821 1.3:+32698593 None:intergenic
GAAGAAAGCAAAGTGGAAAT+TGG 0.294808 1.3:-32698455 MS.gene84048:CDS
GTCATGGATCAAAGAAATTA+AGG 0.294995 1.3:+32700276 None:intergenic
GTTATCATTTGAGCTTCCTT+TGG 0.355140 1.3:+32702119 None:intergenic
TATAAAGAAGGGATCTCTTC+TGG 0.374152 1.3:-32701284 MS.gene84048:CDS
TATAAAGAAGGGATCTCTTA+TGG 0.386855 1.3:-32700936 MS.gene84048:CDS
CCAAAAGTGCAAGCTCTTCA+AGG 0.388417 1.3:-32700861 MS.gene84048:intron
TTTGGTATTTCACTCTCATC+AGG 0.395950 1.3:+32702137 None:intergenic
GAAGCTCAAATGATAACAAA+TGG 0.398449 1.3:-32702113 MS.gene84048:CDS
TGTCATTAAAACTTCAAAGA+AGG 0.419675 1.3:+32701805 None:intergenic
AAAATTGTGGAGTTGAAAAT+GGG 0.422112 1.3:-32698560 MS.gene84048:CDS
TCCAAAAGTGCATGCTCTCC+AGG 0.424580 1.3:-32700481 MS.gene84048:CDS
GTGTTTAAAGTTTCCATTGT+GGG 0.433176 1.3:-32698392 MS.gene84048:CDS
CCAAAAGTGCATGCTCTTCA+GGG 0.454566 1.3:-32700671 MS.gene84048:intron
CGTGGTTGTCAGTTCAGTAA+AGG 0.468915 1.3:-32698494 MS.gene84048:CDS
AACTGACAACCACGAATACT+AGG 0.474382 1.3:+32698503 None:intergenic
ATTTATTATCCTAGTATTCG+TGG 0.474870 1.3:-32698512 MS.gene84048:CDS
TCTTCTAATTCTATGACTAC+TGG 0.476068 1.3:-32701554 MS.gene84048:intron
GTCATTAAAACTTCAAAGAA+GGG 0.479979 1.3:+32701806 None:intergenic
TTACGCCCATGGTCATATCT+TGG 0.485636 1.3:-32701777 MS.gene84048:intron
GAGACACTGGAGAAACTTGT+AGG 0.490241 1.3:-32701238 MS.gene84048:CDS
CCATGACAAGGTTATAAAGA+AGG 0.491612 1.3:-32700758 MS.gene84048:intron
CCATGACAAGGTTATAAAGA+AGG 0.491612 1.3:-32700948 MS.gene84048:intron
CCATGACAAGGTTATAAAGA+AGG 0.491612 1.3:-32701296 MS.gene84048:intron
CCTTCTTTATAACCTTGTCA+TGG 0.501395 1.3:+32700260 None:intergenic
CCATGACAAGGTTATAAAGA+AGG 0.510696 1.3:-32700260 MS.gene84048:CDS
CCAAAGAGATTTATGGAAAG+AGG 0.511759 1.3:-32698593 MS.gene84048:CDS
AAGATGCCGGAGAAACTTGT+AGG 0.513621 1.3:-32700890 MS.gene84048:CDS
AAGCTCAAATGATAACAAAT+GGG 0.514121 1.3:-32702112 MS.gene84048:CDS
AAGACATTGGAGAAACTTGT+AGG 0.516345 1.3:-32700700 MS.gene84048:CDS
AAGACATTGGAGAAACATCT+AGG 0.517707 1.3:-32700202 MS.gene84048:CDS
TCATACTTGAACAAATTTGA+AGG 0.519341 1.3:-32701644 MS.gene84048:CDS
CATACTTGAACAAATTTGAA+GGG 0.523429 1.3:-32701643 MS.gene84048:CDS
AAGGGTCCTACAAGTTTCTC+CGG 0.528798 1.3:+32700884 None:intergenic
GGGATTGCAAGCTTAAGAGT+TGG 0.529065 1.3:-32701623 MS.gene84048:CDS
ATGATCATGATATTCTAAAG+AGG 0.534079 1.3:-32700724 MS.gene84048:CDS
ATTCTAAAGAGGAGAGACAC+TGG 0.536518 1.3:-32700522 MS.gene84048:intron
ATTCTAAAGAGGAGAGACAC+TGG 0.536518 1.3:-32701251 MS.gene84048:CDS
GTTGTCAGTTCAGTAAAGGT+TGG 0.536590 1.3:-32698490 MS.gene84048:CDS
GTGATAAAGATGTTCTAAAG+AGG 0.539402 1.3:-32700914 MS.gene84048:CDS
TGCAAGCTTAAGAGTTGGAG+AGG 0.542791 1.3:-32701618 MS.gene84048:CDS
ATTCTAAAGAGGAAAGACAT+TGG 0.551775 1.3:-32700713 MS.gene84048:CDS
CATGACAAGGTTATAAAGAA+GGG 0.554767 1.3:-32700757 MS.gene84048:intron
CATGACAAGGTTATAAAGAA+GGG 0.554767 1.3:-32700947 MS.gene84048:intron
CATGACAAGGTTATAAAGAA+GGG 0.554767 1.3:-32701295 MS.gene84048:intron
GAGACACTGGAGAAATTTGT+CGG 0.563866 1.3:-32700509 MS.gene84048:CDS
AGAGGAGACAGCTATGAAAG+TGG 0.577915 1.3:-32701600 MS.gene84048:CDS
TATGATCTTGAAAAGTTGCA+TGG 0.578670 1.3:+32701662 None:intergenic
ATTCTACCGTGGAAAGACAT+TGG 0.588028 1.3:-32700215 MS.gene84048:CDS
CCAAAAGAGCATGCTTCCCA+TGG 0.588789 1.3:-32700173 MS.gene84048:intron
GAAATACTTGTCTCCTAGAG+CGG 0.589908 1.3:-32698433 MS.gene84048:CDS
GTGATCGTGATATTCTAAAG+AGG 0.591830 1.3:-32701262 MS.gene84048:CDS
ATGACACAAACTTACGCCCA+TGG 0.594951 1.3:-32701788 MS.gene84048:CDS
AATTTCTTTGATCCATGACA+AGG 0.598171 1.3:-32700272 MS.gene84048:CDS
CCAAAAGTGCATGCTCTCCA+GGG 0.599112 1.3:-32700480 MS.gene84048:intron
GTTCTAAAGAGGAAAGATGC+CGG 0.601662 1.3:-32700903 MS.gene84048:CDS
GTTTCTCCAATGTCTTTCCA+CGG 0.615143 1.3:+32700209 None:intergenic
ATTTATGGAAGAAAGCAAAG+TGG 0.622753 1.3:-32698462 MS.gene84048:CDS
AAATTGTGGAGTTGAAAATG+GGG 0.628496 1.3:-32698559 MS.gene84048:CDS
AATTGTGGAGTTGAAAATGG+GGG 0.632867 1.3:-32698558 MS.gene84048:CDS
TGATGAGAGTGAAATACCAA+AGG 0.651200 1.3:-32702135 MS.gene84048:CDS
ATTGTGGAGTTGAAAATGGG+GGG 0.654265 1.3:-32698557 MS.gene84048:CDS
TGTGTTTAAAGTTTCCATTG+TGG 0.655197 1.3:-32698393 MS.gene84048:CDS
ACTTACCAAGATATGACCAT+GGG 0.667874 1.3:+32701772 None:intergenic
TATAAGGTTCTATCCCACAA+TGG 0.669677 1.3:+32698379 None:intergenic
TACTTACCAAGATATGACCA+TGG 0.678715 1.3:+32701771 None:intergenic
GTGATAAGCATATTCTACCG+TGG 0.706198 1.3:-32700226 MS.gene84048:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAATATATTATAAAAT+AGG - chr1.3:32701670-32701689 MS.gene84048:CDS 0.0%
!!! ATTTTATAATATATTTTTAT+AGG + chr1.3:32701670-32701689 None:intergenic 0.0%
!!! TTTTATAATATATTTTTATA+GGG + chr1.3:32701669-32701688 None:intergenic 0.0%
!! ACAAAATATTTAATAATTCA+AGG - chr1.3:32701525-32701544 MS.gene84048:intron 10.0%
!! ATAAAAAATATATAACCATT+TGG - chr1.3:32701772-32701791 MS.gene84048:intron 10.0%
!! ATAAAAATAATAATCTAAAG+AGG - chr1.3:32701228-32701247 MS.gene84048:CDS 10.0%
!! TAAAATTTCTTTCAAATTTA+TGG + chr1.3:32700818-32700837 None:intergenic 10.0%
!! TTAACTAAATAAAGAAATTA+AGG + chr1.3:32699580-32699599 None:intergenic 10.0%
!!! AAAAATCTCTAATTTTTTTT+GGG - chr1.3:32701642-32701661 MS.gene84048:CDS 10.0%
!!! AAAATCTCTAATTTTTTTTT+GGG - chr1.3:32701700-32701719 MS.gene84048:intron 10.0%
!!! AAAATTAGAGATTTTTAAAT+AGG + chr1.3:32701638-32701657 None:intergenic 10.0%
!!! AATTAAAATTGTTATTAATC+TGG - chr1.3:32700664-32700683 MS.gene84048:intron 10.0%
!!! ATAATGTATAAATGTTATTT+TGG - chr1.3:32702096-32702115 MS.gene84048:CDS 10.0%
!!! CTATTTAAAATTAATGTTTA+AGG + chr1.3:32700795-32700814 None:intergenic 10.0%
!!! GAATTATTAAATATTTTGTA+AGG + chr1.3:32701524-32701543 None:intergenic 10.0%
!!! TAAAAATCTCTAATTTTTTT+TGG - chr1.3:32701641-32701660 MS.gene84048:CDS 10.0%
!!! TAAAATCTCTAATTTTTTTT+TGG - chr1.3:32701699-32701718 MS.gene84048:intron 10.0%
!!! TTAATTTTTAAATATATTGG+TGG - chr1.3:32698453-32698472 MS.gene84048:CDS 10.0%
!! AAAAACTATTGATATGTTTA+AGG - chr1.3:32701582-32701601 MS.gene84048:CDS 15.0%
!! AAAACTATTGATATGTTTAA+GGG - chr1.3:32701583-32701602 MS.gene84048:CDS 15.0%
!! CAAAATATTAAATAGTTCAA+GGG - chr1.3:32701080-32701099 MS.gene84048:intron 15.0%
!! TAAATTATTGATAAGTTTAG+AGG + chr1.3:32700690-32700709 None:intergenic 15.0%
!! TTTCTAATATATAAGTTTAG+AGG + chr1.3:32701409-32701428 None:intergenic 15.0%
!!! AAACATTTTCAAAGAAATTA+AGG + chr1.3:32701030-32701049 None:intergenic 15.0%
!!! AAAGATTTAGAGATTTTTAA+AGG + chr1.3:32701728-32701747 None:intergenic 15.0%
!!! ACTTTTAAATAATTTGTGAA+TGG - chr1.3:32701497-32701516 MS.gene84048:intron 15.0%
!!! AGAAAAATCCATTTTTATAT+TGG - chr1.3:32701424-32701443 MS.gene84048:intron 15.0%
!!! ATAAGTAAATTCATTTTCAA+AGG + chr1.3:32698733-32698752 None:intergenic 15.0%
!!! ATATTTGGTTTCAAAAAAAT+TGG - chr1.3:32698394-32698413 MS.gene84048:CDS 15.0%
!!! CATATCAATAGTTTTTATAT+AGG + chr1.3:32701578-32701597 None:intergenic 15.0%
!!! CTTTTAAATAATTTGTGAAT+GGG - chr1.3:32701498-32701517 MS.gene84048:intron 15.0%
!!! GAAAAATCCATTTTTATATT+GGG - chr1.3:32701425-32701444 MS.gene84048:intron 15.0%
!!! TATTTGGTTTCAAAAAAATT+GGG - chr1.3:32698395-32698414 MS.gene84048:CDS 15.0%
!!! TTGATAATAATATTCTAAAG+AGG - chr1.3:32700198-32700217 MS.gene84048:CDS 15.0%
!!! TTTAAGGATACATTTTTTTA+GGG + chr1.3:32700754-32700773 None:intergenic 15.0%
!! AAAATATATAACCATTTGGT+TGG - chr1.3:32701776-32701795 MS.gene84048:intron 20.0%
!! AAGGAAACATGATATTAAAA+AGG + chr1.3:32698714-32698733 None:intergenic 20.0%
!! ACAAATTATTGATGTGTTTA+AGG - chr1.3:32700937-32700956 MS.gene84048:CDS 20.0%
!! AGGGTTAATTACATAAAATA+TGG - chr1.3:32702034-32702053 MS.gene84048:intron 20.0%
!! ATAGTAATAGTGAATAAAAG+TGG + chr1.3:32699276-32699295 None:intergenic 20.0%
!! CAAAAATATAACTTAGAAGT+TGG - chr1.3:32699216-32699235 MS.gene84048:intron 20.0%
!! CAGTAATCTATAATAAAATG+TGG - chr1.3:32699023-32699042 MS.gene84048:intron 20.0%
!! GCAAAATATTAAATAGTTCA+AGG - chr1.3:32701079-32701098 MS.gene84048:intron 20.0%
!! TAAAGGTTGGATAAAATTTA+TGG - chr1.3:32702254-32702273 MS.gene84048:intron 20.0%
!! TATTTCAAGGTTAAGATATT+TGG - chr1.3:32698480-32698499 MS.gene84048:CDS 20.0%
!! TCTAAAAATGTCTACTAATT+TGG - chr1.3:32698987-32699006 MS.gene84048:intron 20.0%
!! TTGAACCTTAGAAAATATTT+AGG - chr1.3:32700877-32700896 MS.gene84048:CDS 20.0%
!!! ACATTTTTTTAGGGAATTTA+AGG + chr1.3:32700745-32700764 None:intergenic 20.0%
!!! CAACTTCTAAGTTATATTTT+TGG + chr1.3:32699218-32699237 None:intergenic 20.0%
!!! CACATCAATAATTTGTATAT+AGG + chr1.3:32700933-32700952 None:intergenic 20.0%
!!! CATCTTTTTGAATTGTTATT+TGG - chr1.3:32701141-32701160 MS.gene84048:intron 20.0%
!!! GAATTCCTAAATATTTTCTA+AGG + chr1.3:32700885-32700904 None:intergenic 20.0%
!!! GTTTAAGGATACATTTTTTT+AGG + chr1.3:32700755-32700774 None:intergenic 20.0%
!!! TCAGTAAATTAATTTTTCGT+TGG - chr1.3:32701550-32701569 MS.gene84048:intron 20.0%
!!! TCATTTTCCCAATATAAAAA+TGG + chr1.3:32701435-32701454 None:intergenic 20.0%
!!! TTCATCAACAGATTTTATAT+TGG + chr1.3:32698532-32698551 None:intergenic 20.0%
!!! TTTAGATTTTACTATTGTGA+AGG - chr1.3:32701331-32701350 MS.gene84048:intron 20.0%
!!! TTTTTATATTGGGAAAATGA+AGG - chr1.3:32701435-32701454 MS.gene84048:intron 20.0%
! AAAAATTGTGGAGTTGAAAA+TGG - chr1.3:32702170-32702189 MS.gene84048:CDS 25.0%
! AAAATTGTGGAGTTGAAAAT+GGG - chr1.3:32702171-32702190 MS.gene84048:CDS 25.0%
! AAAGTTTGAAGATTATACCA+TGG + chr1.3:32700578-32700597 None:intergenic 25.0%
! AACTTCTTTATAACCTTGTA+AGG + chr1.3:32700168-32700187 None:intergenic 25.0%
! AAGCTCAAATGATAACAAAT+GGG - chr1.3:32698619-32698638 MS.gene84048:intron 25.0%
! AAGTTTGAAGATTATACCAT+GGG + chr1.3:32700577-32700596 None:intergenic 25.0%
! AATTTCTTTGATCCATAACA+AGG - chr1.3:32699614-32699633 MS.gene84048:intron 25.0%
! ACTTCTTTATAACCTTGTAA+GGG + chr1.3:32700167-32700186 None:intergenic 25.0%
! ATTTATTATCCTAGTATTCG+TGG - chr1.3:32702219-32702238 MS.gene84048:CDS 25.0%
! CAGTGTTCAGTTATTATTTA+TGG - chr1.3:32700905-32700924 MS.gene84048:CDS 25.0%
! CATAAATAATAACTGAACAC+TGG + chr1.3:32700907-32700926 None:intergenic 25.0%
! CATACTTGAACAAATTTGAA+GGG - chr1.3:32699088-32699107 MS.gene84048:intron 25.0%
! GTCATGGATCAAAAAATTTA+AGG + chr1.3:32699770-32699789 None:intergenic 25.0%
! GTCATTAAAACTTCAAAGAA+GGG + chr1.3:32698928-32698947 None:intergenic 25.0%
! GTTATGGATCAAAGAAATTA+AGG + chr1.3:32699613-32699632 None:intergenic 25.0%
! TCATACTTGAACAAATTTGA+AGG - chr1.3:32699087-32699106 MS.gene84048:intron 25.0%
! TCTTCTTTATAACCTTGTTA+TGG + chr1.3:32699629-32699648 None:intergenic 25.0%
! TGTCATTAAAACTTCAAAGA+AGG + chr1.3:32698929-32698948 None:intergenic 25.0%
!! ATGATCATGATATTCTAAAG+AGG - chr1.3:32700007-32700026 MS.gene84048:intron 25.0%
!! ATTTTGTGTGTAGAATCAAA+GGG - chr1.3:32701954-32701973 MS.gene84048:intron 25.0%
!! TTTTATGTAATTAACCCTAC+CGG + chr1.3:32702032-32702051 None:intergenic 25.0%
!!! AAATTTTTTGATCCATGACA+AGG - chr1.3:32699771-32699790 MS.gene84048:intron 25.0%
!!! ACATTTTTTTAGGGAGTTTA+AGG + chr1.3:32700770-32700789 None:intergenic 25.0%
AAAAAACCTTGCAACTTCTT+AGG + chr1.3:32699324-32699343 None:intergenic 30.0%
AAAAACCTTGCAACTTCTTA+GGG + chr1.3:32699323-32699342 None:intergenic 30.0%
AAAGAAGGGATTCTCATTAT+TGG + chr1.3:32698914-32698933 None:intergenic 30.0%
AAATTGTGGAGTTGAAAATG+GGG - chr1.3:32702172-32702191 MS.gene84048:CDS 30.0%
AAGAATTTAGAAGAATACCC+TGG + chr1.3:32700271-32700290 None:intergenic 30.0%
AATCTAAAGAGGAAATTCGT+TGG - chr1.3:32701239-32701258 MS.gene84048:CDS 30.0%
AATTTCTTTGATCCATGACA+AGG - chr1.3:32699961-32699980 MS.gene84048:intron 30.0%
AATTTCTTTGATCCCTTACA+AGG - chr1.3:32700152-32700171 MS.gene84048:intron 30.0%
ACTATTACTAAAGCCATCAA+GGG - chr1.3:32699291-32699310 MS.gene84048:intron 30.0%
ATTTATGGAAGAAAGCAAAG+TGG - chr1.3:32702269-32702288 MS.gene84048:intron 30.0%
CATGACAAGGTTATAAAGAA+GGG - chr1.3:32699436-32699455 MS.gene84048:intron 30.0%
CATGTCAAGGTTATAAAGAA+AGG - chr1.3:32701195-32701214 MS.gene84048:intron 30.0%
CTTTCTTTATAACCTTGACA+TGG + chr1.3:32701197-32701216 None:intergenic 30.0%
GAAGCTCAAATGATAACAAA+TGG - chr1.3:32698618-32698637 MS.gene84048:intron 30.0%
GATGAAACACAATACATTCA+AGG - chr1.3:32701986-32702005 MS.gene84048:intron 30.0%
GTAAGGGATCAAAGAAATTA+AGG + chr1.3:32700151-32700170 None:intergenic 30.0%
GTCATGGATCAAAGAAATTA+AGG + chr1.3:32699960-32699979 None:intergenic 30.0%
TACTATTACTAAAGCCATCA+AGG - chr1.3:32699290-32699309 MS.gene84048:intron 30.0%
TATAAAGAAGGGATCTCTTA+TGG - chr1.3:32699795-32699814 MS.gene84048:intron 30.0%
TATGATCTTGAAAAGTTGCA+TGG + chr1.3:32699072-32699091 None:intergenic 30.0%
TCTTCTAATTCTATGACTAC+TGG - chr1.3:32699177-32699196 MS.gene84048:intron 30.0%
TGTCATGGATCAAAGAAAAT+AGG + chr1.3:32699423-32699442 None:intergenic 30.0%
TGTGGTTCATGAATTGAAAT+AGG - chr1.3:32699041-32699060 MS.gene84048:intron 30.0%
TTTATGTAATTAACCCTACC+GGG + chr1.3:32702031-32702050 None:intergenic 30.0%
TTTGCAAAGTCTCTCAAATA+TGG - chr1.3:32698423-32698442 MS.gene84048:CDS 30.0%
! ACACAATACATTCAAGGTTT+TGG - chr1.3:32701992-32702011 MS.gene84048:intron 30.0%
! ATTCTAAAGAGGAAAGACAT+TGG - chr1.3:32700018-32700037 MS.gene84048:intron 30.0%
! ATTTTCTTTGATCCATGACA+AGG - chr1.3:32699423-32699442 MS.gene84048:intron 30.0%
! CATTTTGTGTGTAGAATCAA+AGG - chr1.3:32701953-32701972 MS.gene84048:intron 30.0%
! GTGTTTAAAGTTTCCATTGT+GGG - chr1.3:32702339-32702358 MS.gene84048:intron 30.0%
! TATTGGTGGATCATATTTCA+AGG - chr1.3:32698467-32698486 MS.gene84048:CDS 30.0%
! TCTCAAGTGTCAATTTTTGT+AGG - chr1.3:32701835-32701854 MS.gene84048:CDS 30.0%
! TGTGTTTAAAGTTTCCATTG+TGG - chr1.3:32702338-32702357 MS.gene84048:intron 30.0%
!! GTGATAAAGATGTTCTAAAG+AGG - chr1.3:32699817-32699836 MS.gene84048:intron 30.0%
!! TAACAAATGAACTCTAAAGC+AGG - chr1.3:32698820-32698839 MS.gene84048:intron 30.0%
AAGACATTGGAGAAACATCT+AGG - chr1.3:32700529-32700548 MS.gene84048:intron 35.0%
AAGACATTGGAGAAACTTGT+AGG - chr1.3:32700031-32700050 MS.gene84048:intron 35.0%
AATCAAATGAAGTTCTCACC+TGG + chr1.3:32698690-32698709 None:intergenic 35.0%
AATTCGTTGGAGAATCTTGT+AGG - chr1.3:32701252-32701271 MS.gene84048:CDS 35.0%
AATTGTGGAGTTGAAAATGG+GGG - chr1.3:32702173-32702192 MS.gene84048:CDS 35.0%
ACTCTTAAGCAAAAATTCCC+AGG - chr1.3:32698669-32698688 MS.gene84048:intron 35.0%
ACTTACCAAGATATGACCAT+GGG + chr1.3:32698962-32698981 None:intergenic 35.0%
AGGATGTCGTGAAAAAATTG+TGG - chr1.3:32702158-32702177 MS.gene84048:CDS 35.0%
ATCAAATGAAGTTCTCACCT+GGG + chr1.3:32698689-32698708 None:intergenic 35.0%
CCAAAGAGATTTATGGAAAG+AGG - chr1.3:32702138-32702157 MS.gene84048:CDS 35.0%
CCATGACAAGGTTATAAAGA+AGG - chr1.3:32699435-32699454 MS.gene84048:intron 35.0%
CCTCTTTCCATAAATCTCTT+TGG + chr1.3:32702141-32702160 None:intergenic 35.0%
CCTTCTTTATAACCTTGTCA+TGG + chr1.3:32699438-32699457 None:intergenic 35.0%
CTAATTCTATGACTACTGGT+TGG - chr1.3:32699181-32699200 MS.gene84048:intron 35.0%
GAAGAAAGCAAAGTGGAAAT+TGG - chr1.3:32702276-32702295 MS.gene84048:intron 35.0%
GACATGGATCAAAGAAATCA+AGG + chr1.3:32701181-32701200 None:intergenic 35.0%
GATTTCTTTGATCCATGTCA+AGG - chr1.3:32701182-32701201 MS.gene84048:intron 35.0%
GTTATCATTTGAGCTTCCTT+TGG + chr1.3:32698615-32698634 None:intergenic 35.0%
TACTTACCAAGATATGACCA+TGG + chr1.3:32698963-32698982 None:intergenic 35.0%
TATAAAGAAGGGATCTCTTC+TGG - chr1.3:32699447-32699466 MS.gene84048:intron 35.0%
TGATGAGAGTGAAATACCAA+AGG - chr1.3:32698596-32698615 MS.gene84048:CDS 35.0%
TTTGGTAAATTCCAAACACC+CGG - chr1.3:32702010-32702029 MS.gene84048:intron 35.0%
TTTGGTATTTCACTCTCATC+AGG + chr1.3:32698597-32698616 None:intergenic 35.0%
! GTGATCGTGATATTCTAAAG+AGG - chr1.3:32699469-32699488 MS.gene84048:intron 35.0%
! GTGATCGTGATATTCTAAAG+TGG - chr1.3:32699469-32699488 MS.gene84048:intron 35.0%
!! AAATGAACTCTAAAGCAGGT+AGG - chr1.3:32698824-32698843 MS.gene84048:intron 35.0%
!! AAGAGCATGCATTTTTGGAA+GGG + chr1.3:32699520-32699539 None:intergenic 35.0%
AACTGACAACCACGAATACT+AGG + chr1.3:32702231-32702250 None:intergenic 40.0%
AAGACACTCGAGAAACTTGT+AGG - chr1.3:32699684-32699703 MS.gene84048:intron 40.0%
AGACTGTCAAACCAACCAAA+TGG + chr1.3:32701790-32701809 None:intergenic 40.0%
ATTCTACCGTGGAAAGACAT+TGG - chr1.3:32700516-32700535 MS.gene84048:CDS 40.0%
ATTGTGGAGTTGAAAATGGG+GGG - chr1.3:32702174-32702193 MS.gene84048:CDS 40.0%
CCAAAAATGCATGCTCTTCA+GGG - chr1.3:32699522-32699541 MS.gene84048:intron 40.0%
GAAATACTTGTCTCCTAGAG+CGG - chr1.3:32702298-32702317 MS.gene84048:intron 40.0%
GAGACACTGGAGAAATTTGT+CGG - chr1.3:32700222-32700241 MS.gene84048:CDS 40.0%
GCATGCACCAAAGAGATTTA+TGG - chr1.3:32702131-32702150 MS.gene84048:CDS 40.0%
GTGATAAGCATATTCTACCG+TGG - chr1.3:32700505-32700524 MS.gene84048:CDS 40.0%
GTTGTCAGTTCAGTAAAGGT+TGG - chr1.3:32702241-32702260 MS.gene84048:CDS 40.0%
GTTTCTCCAATGTCTTTCCA+CGG + chr1.3:32700525-32700544 None:intergenic 40.0%
TAAATTCCAAACACCCGGTA+GGG - chr1.3:32702015-32702034 MS.gene84048:intron 40.0%
TCCAAAAATGCATGCTCTTC+AGG - chr1.3:32699521-32699540 MS.gene84048:intron 40.0%
! ATTCTAAAGAGGAGAGACAC+TGG - chr1.3:32699480-32699499 MS.gene84048:intron 40.0%
! CTCTGTAGATCATGCAGTTT+TGG + chr1.3:32701284-32701303 None:intergenic 40.0%
! TTTTCTCATCAATCCGCTCT+AGG + chr1.3:32702314-32702333 None:intergenic 40.0%
!! AAGAGCATGCACTTTTGGAA+GGG + chr1.3:32699710-32699729 None:intergenic 40.0%
!! AAGAGCTTGCACTTTTGGAA+GGG + chr1.3:32699868-32699887 None:intergenic 40.0%
!! GAAGAGCATGCATTTTTGGA+AGG + chr1.3:32699521-32699540 None:intergenic 40.0%
!! GTTCTAAAGAGGAAAGATGC+CGG - chr1.3:32699828-32699847 MS.gene84048:intron 40.0%
!! TTTGGTTGGTTTGACAGTCT+TGG - chr1.3:32701790-32701809 MS.gene84048:CDS 40.0%
!!! GTAGATCATGCAGTTTTGGA+CGG + chr1.3:32701280-32701299 None:intergenic 40.0%
AAGATGCCGGAGAAACTTGT+AGG - chr1.3:32699841-32699860 MS.gene84048:intron 45.0%
AAGGGTCCTACAAGTTTCTC+CGG + chr1.3:32699502-32699521 None:intergenic 45.0%
AATTAACCCTACCGGGTGTT+TGG + chr1.3:32702024-32702043 None:intergenic 45.0%
AGAGGAGACAGCTATGAAAG+TGG - chr1.3:32699131-32699150 MS.gene84048:intron 45.0%
ATGACACAAACTTACGCCCA+TGG - chr1.3:32698943-32698962 MS.gene84048:intron 45.0%
CCAAACCCTAAGAAGTTGCA+AGG - chr1.3:32699315-32699334 MS.gene84048:intron 45.0%
CCTTGCAACTTCTTAGGGTT+TGG + chr1.3:32699318-32699337 None:intergenic 45.0%
CGTGGTTGTCAGTTCAGTAA+AGG - chr1.3:32702237-32702256 MS.gene84048:CDS 45.0%
GAGACACTGGAGAAACTTGT+AGG - chr1.3:32699493-32699512 MS.gene84048:intron 45.0%
GGGATTGCAAGCTTAAGAGT+TGG - chr1.3:32699108-32699127 MS.gene84048:intron 45.0%
GTAAATTCCAAACACCCGGT+AGG - chr1.3:32702014-32702033 MS.gene84048:intron 45.0%
TGAAAATGGGGGGACAATCA+TGG - chr1.3:32702184-32702203 MS.gene84048:CDS 45.0%
TGCAAGCTTAAGAGTTGGAG+AGG - chr1.3:32699113-32699132 MS.gene84048:intron 45.0%
TTACGCCCATGGTCATATCT+TGG - chr1.3:32698954-32698973 MS.gene84048:intron 45.0%
! CCAAAAGTGCAAGCTCTTCA+AGG - chr1.3:32699870-32699889 MS.gene84048:intron 45.0%
! CCAAAAGTGCATGCTCTTCA+GGG - chr1.3:32699712-32699731 MS.gene84048:intron 45.0%
! CCTTGAAGAGCTTGCACTTT+TGG + chr1.3:32699873-32699892 None:intergenic 45.0%
! GAAGAGCTTGCACTTTTGGA+AGG + chr1.3:32699869-32699888 None:intergenic 45.0%
! TCCAAAAGTGCATGCTCTTC+AGG - chr1.3:32699711-32699730 MS.gene84048:intron 45.0%
!! CCCTGAAGAGCATGCATTTT+TGG + chr1.3:32699525-32699544 None:intergenic 45.0%
!! GAAGAGCATGCACTTTTGGA+AGG + chr1.3:32699711-32699730 None:intergenic 45.0%
!!! ATTTATTTGTAATTTTTTTA+TGG - chr1.3:32700393-32700412 MS.gene84048:intron 5.0%
!!! CTTTTAATTTTTAAATATAT+TGG - chr1.3:32698450-32698469 MS.gene84048:CDS 5.0%
! CCAAAAGAGCATGCTTCCCA+TGG - chr1.3:32700558-32700577 MS.gene84048:intron 50.0%
! CCAAAAGTGCATGCTCTCCA+GGG - chr1.3:32700251-32700270 MS.gene84048:CDS 50.0%
! CTTAGGGTTTGGTCCCTTGA+TGG + chr1.3:32699307-32699326 None:intergenic 50.0%
! TCCAAAAGTGCATGCTCTCC+AGG - chr1.3:32700250-32700269 MS.gene84048:CDS 50.0%
!! CCATGGGAAGCATGCTCTTT+TGG + chr1.3:32700561-32700580 None:intergenic 50.0%
!! CCCTGAAGAGCATGCACTTT+TGG + chr1.3:32699715-32699734 None:intergenic 50.0%
!! GGAGAGCATGCACTTTTGGA+CGG + chr1.3:32700250-32700269 None:intergenic 50.0%
!! CCCTGGAGAGCATGCACTTT+TGG + chr1.3:32700254-32700273 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 32698388 32702365 32698388 ID=MS.gene84048
chr1.3 mRNA 32698388 32702365 32698388 ID=MS.gene84048.t1;Parent=MS.gene84048
chr1.3 exon 32702363 32702365 32702363 ID=MS.gene84048.t1.exon1;Parent=MS.gene84048.t1
chr1.3 CDS 32702363 32702365 32702363 ID=cds.MS.gene84048.t1;Parent=MS.gene84048.t1
chr1.3 exon 32702063 32702264 32702063 ID=MS.gene84048.t1.exon2;Parent=MS.gene84048.t1
chr1.3 CDS 32702063 32702264 32702063 ID=cds.MS.gene84048.t1;Parent=MS.gene84048.t1
chr1.3 exon 32701778 32701911 32701778 ID=MS.gene84048.t1.exon3;Parent=MS.gene84048.t1
chr1.3 CDS 32701778 32701911 32701778 ID=cds.MS.gene84048.t1;Parent=MS.gene84048.t1
chr1.3 exon 32701555 32701690 32701555 ID=MS.gene84048.t1.exon4;Parent=MS.gene84048.t1
chr1.3 CDS 32701555 32701690 32701555 ID=cds.MS.gene84048.t1;Parent=MS.gene84048.t1
chr1.3 exon 32701210 32701308 32701210 ID=MS.gene84048.t1.exon5;Parent=MS.gene84048.t1
chr1.3 CDS 32701210 32701308 32701210 ID=cds.MS.gene84048.t1;Parent=MS.gene84048.t1
chr1.3 exon 32700862 32700960 32700862 ID=MS.gene84048.t1.exon6;Parent=MS.gene84048.t1
chr1.3 CDS 32700862 32700960 32700862 ID=cds.MS.gene84048.t1;Parent=MS.gene84048.t1
chr1.3 exon 32700672 32700770 32700672 ID=MS.gene84048.t1.exon7;Parent=MS.gene84048.t1
chr1.3 CDS 32700672 32700770 32700672 ID=cds.MS.gene84048.t1;Parent=MS.gene84048.t1
chr1.3 exon 32700481 32700535 32700481 ID=MS.gene84048.t1.exon8;Parent=MS.gene84048.t1
chr1.3 CDS 32700481 32700535 32700481 ID=cds.MS.gene84048.t1;Parent=MS.gene84048.t1
chr1.3 exon 32700174 32700300 32700174 ID=MS.gene84048.t1.exon9;Parent=MS.gene84048.t1
chr1.3 CDS 32700174 32700300 32700174 ID=cds.MS.gene84048.t1;Parent=MS.gene84048.t1
chr1.3 exon 32698388 32698621 32698388 ID=MS.gene84048.t1.exon10;Parent=MS.gene84048.t1
chr1.3 CDS 32698388 32698621 32698388 ID=cds.MS.gene84048.t1;Parent=MS.gene84048.t1
Gene Sequence

>MS.gene84048

ATGGTGATATTTGGTTTCAAAAAAATTGGGATAATTTTGCAAAGTCTCTCAAATATGGAAATCTTTTAATTTTTAAATATATTGGTGGATCATATTTCAAGGTTAAGATATTTGGTGTCAATGCTTTAGAAATAGATTATTCCAATATAAAATCTGTTGATGAAGTTGTTGAAGCTAGAAAAGAAGATAAAGAAATTGTTGATTTGCCTGATGAGAGTGAAATACCAAAGGAAGCTCAAATGATAACAAATGGGAAGAGAAAAATGAGTATAGATATTGACACTCTTAAGCAAAAATTCCCAGGTGAGAACTTCATTTGATTTCCTTTTTAATATCATGTTTCCTTTGAAAATGAATTTACTTATAAAAATTTTATATCAAATAATTTTTAGCAAAATAACTTCTTTTTTTTCTTATAGTATTTTTAGTACTTAACAAATGAACTCTAAAGCAGGTAGGAACATTGAAGACACAATTAAGAAAGTTAAAAAATGCTCTAGAATTGAAGTTGTTAATGAGACAACCAATAATGAGAATCCCTTCTTTGAAGTTTTAATGACACAAACTTACGCCCATGGTCATATCTTGGTAAGTAACTATCTAAAAATGTCTACTAATTTGGCTTTAATTAATATCAGTAATCTATAATAAAATGTGGTTCATGAATTGAAATAGGTGATACCATGCAACTTTTCAAGATCATACTTGAACAAATTTGAAGGGATTGCAAGCTTAAGAGTTGGAGAGGAGACAGCTATGAAAGTGGATATAGTGTTTAACAATAAAAGATCTTCTAATTCTATGACTACTGGTTGGAAGTCATTTAACCAAAAATATAACTTAGAAGTTGGTGATATATGTAAATTTGTGATGACTCAAAGAGAACCACTTTTATTCACTATTACTATTACTAAAGCCATCAAGGGACCAAACCCTAAGAAGTTGCAAGGTTTTTTTCTTTCTTGCTTTGCTTTTTTATTTTTGTCATTAAAAATGTTTATATATTGATATATTTTAATTAACATCTAAATTCCTATTTTCTTTGATCCATGACAAGGTTATAAAGAAGGGATCTCTTCTGGTGATCGTGATATTCTAAAGAGGAGAGACACTGGAGAAACTTGTAGGACCCTTCCAAAAATGCATGCTCTTCAGGGTATTTTTCTAACTTCTTTAAAGTTTCTTTGATCCTTAATTTCTTTATTTAGTTAACATCTAAATTCCTTAATTTCTTTGATCCATAACAAGGTTATAAAGAAGAGATCTCTTTTTGTGATCGTGATATTCTAAAGTGGAAAGACACTCGAGAAACTTGTAGGACCTTCCAAAAGTGCATGCTCTTCAGGGTGTTTTTCTAACTTCTTTAAAGTTTCTTTGATCCTTAAATTTTTTGATCCATGACAAGGTTATAAAGAAGGGATCTCTTATGGTGATAAAGATGTTCTAAAGAGGAAAGATGCCGGAGAAACTTGTAGGACCCTTCCAAAAGTGCAAGCTCTTCAAGGTATTTTTTAACTTCTTTAAAGTTTCTTTGATCCTTAATTTCTTTATTTAGTTAACATCTTAATTCCTTAATTTCTTTGATCCATGACAAGGTTATAAAGAAGGGATCTCTTATGATGATCATGATATTCTAAAGAGGAAAGACATTGGAGAAACTTGTAGGACCCTTCCAAAAGTGCATGCTCTTCAGGGTATTTTTCTAACTTCTTTAAAGTTTCTTTGATCCTTAATTTCTTTATTTAGTTAACATATAAATTCCTTAATTTCTTTGATCCCTTACAAGGTTATAAAGAAGTTATCTCTTCTTTTGATAATAATATTCTAAAGAGGAGAGACACTGGAGAAATTTGTCGGAGCCGTCCAAAAGTGCATGCTCTCCAGGGTATTCTTCTAAATTCTTTAAAGATTTTAGATTTTACTATATTGTGAACAATTCTTAAGTATTTGTAGTTTTTTAAATAGTTATCTAAATTAAAAAAATTAAGTTAAAAATGTTTTACTAATTTATTTGTAATTTTTTTATGGAAAAATGTTTTAAATTGATATTTAATTAACATCTAAGTTCCTTAATTTCTTTGATCCATGACAAGGTTATAAAGAAGGCATCTCTTCTTGTGATAAGCATATTCTACCGTGGAAAGACATTGGAGAAACATCTAGGAGCTGTCCAAAAGAGCATGCTTCCCATGGTATAATCTTCAAACTTTAAAGATTTTAGATTTTTCTATTGTGAAGTATTTTTAAATATTTGTAATTTTTTAAATAGTTCTCTGAATTAAAATTGTTATTAATCTGGCCTCTAAACTTATCAATAATTTATACGTTTTTGAACTGAAAGATTTAAAATGTATCCTTAAATTCCCTAAAAAAATGTATCCTTAAACTCCCTAAAAAAATGTATCCTTAAACATTAATTTTAAATAGCCATAAATTTGAAAGAAATTTTAGTTATGTTGTTAAACTATTAGATAATTTGTAATTTGTGATTGAACCTTAGAAAATATTTAGGAATTCCAGTGTTCAGTTATTATTTATGGAACCTATATACAAATTATTGATGTGTTTAAGGACATGATTAGAAATTTATTTAGCTTAAAAATCTATTTAATTTTTTTTATTTGATTTACAACTAAATTCCTTAATTTCTTTGAAAATGTTTTATTATTTTGCTTTGTGTAGAATCGTATCGCAAAATATTAAATAGTTCAAGGGTTCTGTTATTATTTTGCTTTGTTGTTTTTGTCATTAAACATCTTTTTGAATTGTTATTTGGTTTACATCTCAATTCCTTGATTTCTTTGATCCATGTCAAGGTTATAAAGAAAGGATCTCTTCTTATAAAAATAATAATCTAAAGAGGAAATTCGTTGGAGAATCTTGTAGGAGCCGTCCAAAACTGCATGATCTACAGAGTACTATTCTTCTAACTTTTTTAAAGTTTTTAGATTTTACTATTGTGAAGGATTATTAGACATTTGTATTTTTTTTTAAATAGTTCTCTGAATTAAAGTTGATCCTCTAAACTTATATATTAGAAAAATCCATTTTTATATTGGGAAAATGAAGGTTTTTTTTATTAATTCAAGTGACATTAATTAACATTTCAACTTTTAAATAATTTGTGAATGGGCCTTACAAAATATTTAATAATTCAAGGATTCAGTAAATTAATTTTTCGTTGGAACCTATATAAAAACTATTGATATGTTTAAGGGCGTGATTAGAAATTTATTTATATTATAAACCTATTTAAAAATCTCTAATTTTTTTTGGGACCCTATAAAAATATATTATAAAATAGGATATTTTAAAATCTCTAATTTTTTTTTGGGATCCTTTAAAAATCTCTAAATCTTTAAATATATTTTTTCGTAGTATATTATAAAAAATATATAACCATTTGGTTGGTTTGACAGTCTTGGCTTGAGACATAAAGTTTAATTCTCTCAAGTGTCAATTTTTGTAGGCTATTTTGTCTTCTTAGAAAAAGTTTAATTCTCTCAAGTGTCAATTTTTGTAGGCTATTTTAACTTCTTAAAAAAAACATTAATATATATAATATCATTTTGTGTGTAGAATCAAAGGGTCAAAACGTGATGAAACACAATACATTCAAGGTTTTGGTAAATTCCAAACACCCGGTAGGGTTAATTACATAAAATATGGCTACTATGTTGATATATAAAGATATAATGTTAGAACTAAATAATGTATAAATGTTATTTTGGTTTGTATATGCAGCATGCACCAAAGAGATTTATGGAAAGAGGATGTCGTGAAAAAATTGTGGAGTTGAAAATGGGGGGACAATCATGGTTCGTAAAAGTGATTTATTATCCTAGTATTCGTGGTTGTCAGTTCAGTAAAGGTTGGATAAAATTTATGGAAGAAAGCAAAGTGGAAATTGGAAATACTTGTCTCCTAGAGCGGATTGATGAGAAAAAGTATGTGTTTAAAGTTTCCATTGTGGGATAG
Protein sequence

>MS.gene84048.t1

MVKIFGVNALEIDYSNIKSVDEVVEARKEDKEIVDLPDESEIPKEAQMITNGKRKMSIDIDTLKQKFPGRNIEDTIKKVKKCSRIEVVNETTNNENPFFEVLMTQTYAHGHILVIPCNFSRSYLNKFEGIASLRVGEETAMKVDIVFNNKRSSNSMTTGYKEGISSGDRDILKRRDTGETCRTLPKMHALQGYKEGISYGDKDVLKRKDAGETCRTLPKVQALQGYKEGISYDDHDILKRKDIGETCRTLPKVHALQEERHWRNLSEPSKSACSPGSLISLIHDKVIKKASLLVISIFYRGKTLEKHLGAVQKSMLPMHAPKRFMERGCREKIVELKMGGQSWFVKVIYYPSIRGCQFSKGWIKFMEESKVEIGNTCLLERIDEKKYVFKVSIVG