AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84188

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84188.t1 MTR_7g068600 93.277 238 16 0 1 238 1 238 2.01e-167 461
MS.gene84188.t1 MTR_7g088070 55.303 132 53 2 13 141 50 178 1.06e-43 149
MS.gene84188.t1 MTR_7g088070 55.814 129 53 2 13 141 50 174 3.18e-43 147
MS.gene84188.t1 MTR_2g027860 49.045 157 72 5 13 166 49 200 1.36e-42 147
MS.gene84188.t1 MTR_1g053835 50.000 138 63 4 2 138 246 378 8.54e-36 131
MS.gene84188.t1 MTR_7g098250 50.000 144 57 4 1 138 250 384 6.00e-35 130
MS.gene84188.t1 MTR_7g093030 47.287 129 64 2 13 141 212 336 5.58e-34 125
MS.gene84188.t1 MTR_1g080330 43.226 155 80 5 13 166 175 322 4.85e-32 120
MS.gene84188.t1 MTR_2g086450 42.282 149 63 3 13 141 45 190 1.82e-29 114
MS.gene84188.t1 MTR_6g444980 45.802 131 65 2 13 140 35 162 5.52e-29 112
MS.gene84188.t1 MTR_6g032990 42.953 149 66 3 9 141 38 183 5.65e-29 112
MS.gene84188.t1 MTR_6g444980 45.455 132 66 2 13 141 35 163 1.05e-28 111
MS.gene84188.t1 MTR_5g027440 41.958 143 64 3 13 141 25 162 1.13e-28 109
MS.gene84188.t1 MTR_7g115530 42.636 129 71 1 13 141 218 343 6.43e-28 110
MS.gene84188.t1 MTR_4g081710 39.310 145 72 4 9 141 13 153 3.30e-26 104
MS.gene84188.t1 MTR_1g080330 48.214 112 53 3 13 123 175 282 5.66e-25 100
MS.gene84188.t1 MTR_5g041350 38.095 147 72 4 9 141 11 152 7.58e-25 100
MS.gene84188.t1 MTR_2g027800 34.884 172 89 4 9 176 35 187 2.02e-23 96.3
MS.gene84188.t1 MTR_1g053800 43.478 138 72 3 3 138 165 298 2.04e-23 96.7
MS.gene84188.t1 MTR_2g027800 32.778 180 96 3 9 176 35 201 6.76e-23 95.1
MS.gene84188.t1 MTR_7g093030 57.500 80 29 3 13 91 212 287 3.57e-22 92.8
MS.gene84188.t1 MTR_7g115530 42.857 112 61 1 13 124 218 326 3.98e-22 93.2
MS.gene84188.t1 MTR_1g090670 35.256 156 79 3 13 159 44 186 2.57e-20 87.8
MS.gene84188.t1 MTR_7g115530 56.923 65 25 1 13 77 218 279 4.14e-18 81.6
MS.gene84188.t1 MTR_7g115530 56.923 65 25 1 13 77 218 279 1.29e-17 80.9
MS.gene84188.t1 MTR_1g053830 48.101 79 37 2 9 87 14 88 1.72e-16 72.8
MS.gene84188.t1 MTR_7g093010 36.111 144 78 4 1 138 201 336 2.23e-16 77.4
MS.gene84188.t1 MTR_3g450310 34.839 155 89 6 2 149 5 154 3.97e-14 69.3
MS.gene84188.t1 MTR_7g115530 58.182 55 20 1 13 67 218 269 4.36e-14 70.1
MS.gene84188.t1 MTR_7g115530 55.357 56 22 1 13 68 218 270 2.20e-13 68.6
MS.gene84188.t1 MTR_2g084230 59.322 59 21 1 15 73 99 154 4.58e-13 68.2
MS.gene84188.t1 MTR_2g023580 61.818 55 18 1 15 69 57 108 4.80e-13 66.6
MS.gene84188.t1 MTR_2g084230 59.322 59 21 1 15 73 99 154 6.11e-13 67.4
MS.gene84188.t1 MTR_8g086410 57.627 59 22 1 15 73 113 168 7.45e-13 67.8
MS.gene84188.t1 MTR_8g086410 57.627 59 22 1 15 73 111 166 7.78e-13 67.4
MS.gene84188.t1 MTR_7g069660 55.000 60 24 1 15 74 73 129 1.29e-12 66.6
MS.gene84188.t1 MTR_2g016220 57.627 59 22 1 15 73 91 146 1.90e-12 66.2
MS.gene84188.t1 MTR_2g016220 57.627 59 22 1 15 73 91 146 2.03e-12 66.2
MS.gene84188.t1 MTR_6g071625 53.448 58 24 1 15 72 204 258 3.62e-12 65.5
MS.gene84188.t1 MTR_1g021520 53.448 58 24 1 15 72 128 182 5.00e-12 64.7
MS.gene84188.t1 MTR_8g066310 52.542 59 25 1 15 73 175 230 8.01e-12 64.3
MS.gene84188.t1 MTR_3g064840 39.773 88 50 1 15 102 276 360 1.46e-11 63.9
MS.gene84188.t1 MTR_5g029470 53.448 58 24 1 15 72 221 275 3.80e-11 62.4
MS.gene84188.t1 MTR_8g086410 54.545 55 22 1 15 69 113 164 9.90e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84188.t1 AT2G01060 47.305 167 82 4 9 172 11 174 9.46e-46 154
MS.gene84188.t1 AT5G06800 49.275 138 66 2 9 146 188 321 7.37e-38 136
MS.gene84188.t1 AT5G06800 47.887 142 67 2 9 146 188 326 1.67e-37 135
MS.gene84188.t1 AT4G28610 48.529 136 56 3 9 138 221 348 4.82e-33 124
MS.gene84188.t1 AT1G79430 43.506 154 68 4 13 161 34 173 3.94e-32 120
MS.gene84188.t1 AT5G29000 44.286 140 72 3 2 138 177 313 4.66e-32 120
MS.gene84188.t1 AT5G29000 44.286 140 72 3 2 138 177 313 4.66e-32 120
MS.gene84188.t1 AT5G29000 44.286 140 72 3 2 138 220 356 8.07e-32 120
MS.gene84188.t1 AT3G24120 38.690 168 79 3 13 168 41 196 1.63e-30 115
MS.gene84188.t1 AT1G69580 43.046 151 64 4 9 141 30 176 1.64e-30 115
MS.gene84188.t1 AT1G69580 42.763 152 64 4 9 141 30 177 2.52e-30 115
MS.gene84188.t1 AT3G04450 38.012 171 93 5 9 170 194 360 2.03e-29 114
MS.gene84188.t1 AT3G04450 38.012 171 93 5 9 170 194 360 2.03e-29 114
MS.gene84188.t1 AT4G13640 39.412 170 75 4 13 168 37 192 2.48e-29 111
MS.gene84188.t1 AT3G04450 38.012 171 93 5 9 170 234 400 2.94e-29 114
MS.gene84188.t1 AT3G13040 43.537 147 74 3 1 141 228 371 6.53e-29 113
MS.gene84188.t1 AT3G13040 43.537 147 74 3 1 141 228 371 6.53e-29 113
MS.gene84188.t1 AT3G13040 43.537 147 74 3 1 141 228 371 6.53e-29 113
MS.gene84188.t1 AT3G04030 42.953 149 65 4 9 141 41 185 1.72e-28 111
MS.gene84188.t1 AT3G24120 38.012 171 79 4 13 168 41 199 1.77e-28 109
MS.gene84188.t1 AT5G18240 44.828 145 60 5 13 141 45 185 2.17e-28 111
MS.gene84188.t1 AT5G18240 44.828 145 60 5 13 141 45 185 2.17e-28 111
MS.gene84188.t1 AT4G13640 38.728 173 75 5 13 168 37 195 2.56e-27 106
MS.gene84188.t1 AT2G20400 44.094 127 67 2 13 138 231 354 8.48e-27 107
MS.gene84188.t1 AT2G01060 44.000 125 67 3 51 172 1 125 1.08e-26 103
MS.gene84188.t1 AT3G04030 42.282 149 65 5 9 141 41 184 6.74e-26 104
MS.gene84188.t1 AT2G20400 42.063 126 70 1 13 138 231 353 1.33e-25 103
MS.gene84188.t1 AT5G18240 43.448 145 60 6 13 141 45 183 3.02e-25 102
MS.gene84188.t1 AT5G45580 38.194 144 68 3 13 138 7 147 7.38e-25 99.4
MS.gene84188.t1 AT5G45580 38.194 144 68 3 13 138 23 163 9.05e-25 99.4
MS.gene84188.t1 AT3G04030 40.940 149 62 5 9 141 41 179 5.38e-24 99.0
MS.gene84188.t1 AT5G18240 42.759 145 57 6 13 141 45 179 6.56e-24 99.0
MS.gene84188.t1 AT5G18240 42.759 145 57 6 13 141 45 179 6.56e-24 99.0
MS.gene84188.t1 AT3G12730 37.778 135 73 3 13 139 23 154 4.25e-23 94.0
MS.gene84188.t1 AT5G06800 62.319 69 23 1 9 77 188 253 1.65e-22 93.6
MS.gene84188.t1 AT5G29000 47.872 94 46 1 2 95 220 310 7.17e-20 86.7
MS.gene84188.t1 AT3G04450 54.412 68 28 1 9 76 234 298 2.16e-18 82.8
MS.gene84188.t1 AT1G79430 38.655 119 57 3 48 161 1 108 7.35e-18 80.9
MS.gene84188.t1 AT2G42660 61.905 63 21 1 15 77 52 111 6.52e-16 75.1
MS.gene84188.t1 AT2G06020 50.820 61 27 1 13 73 86 143 1.48e-15 74.7
MS.gene84188.t1 AT2G40260 60.345 58 20 1 15 72 84 138 2.87e-14 71.6
MS.gene84188.t1 AT3G04030 39.091 110 50 3 48 141 1 109 3.08e-14 71.2
MS.gene84188.t1 AT5G42630 50.000 74 33 2 15 87 107 177 1.61e-13 67.8
MS.gene84188.t1 AT5G42630 50.000 74 33 2 15 87 107 177 1.70e-13 68.6
MS.gene84188.t1 AT4G04605 51.351 74 32 2 15 88 16 85 1.77e-13 67.8
MS.gene84188.t1 AT4G17695 38.835 103 47 2 15 104 166 265 4.90e-12 64.7
MS.gene84188.t1 AT5G16560 46.575 73 34 2 15 85 221 290 1.63e-11 63.5
MS.gene84188.t1 AT2G38300 55.932 59 23 1 15 73 56 111 2.23e-11 63.2
MS.gene84188.t1 AT1G14600 55.932 59 23 1 15 73 25 80 2.76e-11 62.0
MS.gene84188.t1 AT1G32240 53.448 58 24 1 15 72 215 269 3.05e-11 62.8
MS.gene84188.t1 AT1G32240 53.448 58 24 1 15 72 215 269 3.21e-11 62.8
MS.gene84188.t1 AT2G02060 60.000 55 19 1 15 69 31 82 3.42e-11 62.0
MS.gene84188.t1 AT2G02060 60.000 55 19 1 15 69 31 82 3.42e-11 62.0
MS.gene84188.t1 AT4G37180 51.724 58 25 1 13 70 210 264 9.27e-11 61.2
MS.gene84188.t1 AT4G37180 51.724 58 25 1 13 70 217 271 9.90e-11 61.2

Find 60 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTTACTTGTGGTGGATTC+TGG 0.193661 7.1:+33335476 None:intergenic
GGTGCCTAGTTTCTTATTAT+AGG 0.220382 7.1:-33334348 None:intergenic
ACCAAACCTGGAACCTTTCT+TGG 0.254138 7.1:-33334406 MS.gene84188:CDS
TACGATATAGAACAGAAATT+TGG 0.266828 7.1:+33335324 None:intergenic
GGTGGATTCTGGAATCAATT+TGG 0.287077 7.1:+33335487 None:intergenic
TCTTTGTGGAGGCTGTAAAT+AGG 0.323743 7.1:-33337515 MS.gene84188:CDS
TGGAGGCTGTAAATAGGCTT+GGG 0.332347 7.1:-33337509 MS.gene84188:CDS
TCCAAGAAAGGTTCCAGGTT+TGG 0.364366 7.1:+33334405 None:intergenic
TTGGATATGTTCAGAAATAC+AGG 0.386624 7.1:-33335514 MS.gene84188:intron
GAATAGTGCTCTTCAGCTTA+AGG 0.390190 7.1:-33335229 MS.gene84188:intron
ACACGTCAAAAGCCACTTGC+AGG 0.391889 7.1:-33336356 MS.gene84188:intron
GTGGAGGCTGTAAATAGGCT+TGG 0.397815 7.1:-33337510 MS.gene84188:CDS
AAGAAAAGCTTGCAAGCCTT+TGG 0.401704 7.1:-33334591 MS.gene84188:CDS
CTGACTCCAAGAAAGGTTCC+AGG 0.408019 7.1:+33334400 None:intergenic
GGAAAGTACCTAGACAGAAT+TGG 0.410201 7.1:-33334641 MS.gene84188:CDS
TGCTGTTTGATGTTCTTCCT+CGG 0.424128 7.1:+33334508 None:intergenic
GAACCTTTCTTGGAGTCAGC+TGG 0.440714 7.1:-33334396 MS.gene84188:CDS
ATTCCTTTCAGTATACCTCT+TGG 0.449845 7.1:+33336412 None:intergenic
CAGAATTCAGCAACCAAACC+TGG 0.457761 7.1:-33334418 MS.gene84188:CDS
GTTGCTGAATTCTGAGGTAG+TGG 0.463218 7.1:+33334427 None:intergenic
ACAACTCCATGATTTCTTTG+TGG 0.470072 7.1:-33337529 MS.gene84188:CDS
AACTAGGCACCAAAGGTGAT+TGG 0.478600 7.1:+33334360 None:intergenic
CAGCCTCCACAAAGAAATCA+TGG 0.481607 7.1:+33337523 None:intergenic
CAGCCAGCTGACTCCAAGAA+AGG 0.496169 7.1:+33334393 None:intergenic
AGCAACCTACCAATCACCTT+TGG 0.500347 7.1:-33334369 MS.gene84188:CDS
ACTGAAAGGAATGAAGGCTA+TGG 0.503485 7.1:-33336401 MS.gene84188:CDS
TACTTTGTCCAATTCTGTCT+AGG 0.510514 7.1:+33334633 None:intergenic
CTGAAAGGAATGAAGGCTAT+GGG 0.510802 7.1:-33336400 MS.gene84188:CDS
CACACAAAGAACGCTTAAGA+TGG 0.520735 7.1:-33337557 MS.gene84188:CDS
TCAAACAGCAAGAAAGCAAA+AGG 0.520861 7.1:-33334495 MS.gene84188:CDS
TAAGGAAGTTCTTCAAATGC+AGG 0.521818 7.1:-33335211 MS.gene84188:CDS
ATGGGTTCCACTCGCTCAGA+TGG 0.537642 7.1:-33337588 MS.gene84188:CDS
AGGTATACTGAAAGGAATGA+AGG 0.543020 7.1:-33336407 MS.gene84188:CDS
TTCAGATTCCTCAGTAAGAG+AGG 0.547975 7.1:+33334547 None:intergenic
AAAGCTTGCAAGCCTTTGGT+TGG 0.548496 7.1:-33334587 MS.gene84188:CDS
GAGGCTGTAAATAGGCTTGG+GGG 0.562654 7.1:-33337507 MS.gene84188:CDS
AATAAGAAACTAGGCACCAA+AGG 0.567599 7.1:+33334353 None:intergenic
ACTCCATGATTTCTTTGTGG+AGG 0.569205 7.1:-33337526 MS.gene84188:CDS
GGAGGCTGTAAATAGGCTTG+GGG 0.579180 7.1:-33337508 MS.gene84188:CDS
AAAACCTATAATAAGAAACT+AGG 0.579659 7.1:+33334344 None:intergenic
TCTTAAGCGTTCTTTGTGTG+TGG 0.591577 7.1:+33337560 None:intergenic
GGTTTGGTTGCTGAATTCTG+AGG 0.596881 7.1:+33334421 None:intergenic
GGTGTTGCTTTACCAACCAA+AGG 0.599644 7.1:+33334575 None:intergenic
AGTTCTTCAAATGCAGGCAG+AGG 0.605187 7.1:-33335205 MS.gene84188:CDS
ACCTCTTCCCTCTCTTACTG+AGG 0.608111 7.1:-33334555 MS.gene84188:CDS
GATCCTAGTTCAAAACCTGT+AGG 0.608319 7.1:+33334452 None:intergenic
ATCCTTTAAAACACAATCCG+AGG 0.610019 7.1:-33334525 MS.gene84188:CDS
GATCATCTATGAAGGAGGAA+AGG 0.610342 7.1:-33335376 MS.gene84188:intron
GGCAGAGCCATCTGAGCGAG+TGG 0.612768 7.1:+33337581 None:intergenic
AGGCACCAAAGGTGATTGGT+AGG 0.616341 7.1:+33334364 None:intergenic
ACGCATGAGTGATAAAGTTG+AGG 0.616870 7.1:-33335175 MS.gene84188:intron
TCCTCAGTAAGAGAGGGAAG+AGG 0.625723 7.1:+33334554 None:intergenic
TCAGATTCCTCAGTAAGAGA+GGG 0.627051 7.1:+33334548 None:intergenic
ACACCAAGAGGTATACTGAA+AGG 0.630195 7.1:-33336415 MS.gene84188:CDS
TGAAAGGAATGAAGGCTATG+GGG 0.636336 7.1:-33336399 MS.gene84188:CDS
GAAAGGAATGAAGGCTATGG+GGG 0.636670 7.1:-33336398 MS.gene84188:CDS
TTGTCAGGGGCAACACCAAG+AGG 0.658933 7.1:-33336427 MS.gene84188:intron
GGTGGAGAAGAACCTGCAAG+TGG 0.679561 7.1:+33336344 None:intergenic
AGGCTGAAAATTGAAGCACA+AGG 0.685954 7.1:-33334662 MS.gene84188:CDS
AGGCTGTAAATAGGCTTGGG+GGG 0.689265 7.1:-33337506 MS.gene84188:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAACAATATAATTATTTCTA+TGG - chr7.1:33337156-33337175 MS.gene84188:intron 10.0%
!!! ATATAGTCTTTATAATTTTT+AGG - chr7.1:33337096-33337115 MS.gene84188:intron 10.0%
!!! ATTTTTAAATAAAAAAGAGA+AGG + chr7.1:33335336-33335355 None:intergenic 10.0%
!!! TTTTTAAATTATTAGTTCAA+AGG - chr7.1:33334719-33334738 MS.gene84188:intron 10.0%
!! AATATGCTAATTAGTAAAAA+TGG - chr7.1:33336104-33336123 MS.gene84188:intron 15.0%
!! GTTATAATTTCAATTGAATT+TGG + chr7.1:33336532-33336551 None:intergenic 15.0%
!! TTACTTTATATAAGTCAATT+TGG + chr7.1:33336337-33336356 None:intergenic 15.0%
!!! AAATGTTATTTTGGTATTTT+AGG - chr7.1:33335884-33335903 MS.gene84188:intron 15.0%
!!! AATTTTATGACCAATATAAA+TGG + chr7.1:33335618-33335637 None:intergenic 15.0%
!!! ATTTTATGACCAATATAAAT+GGG + chr7.1:33335617-33335636 None:intergenic 15.0%
!!! CAAAATTTTGTATTATTTGT+TGG - chr7.1:33337234-33337253 MS.gene84188:intron 15.0%
!!! TATGATATTTTAATTTGTCA+AGG - chr7.1:33336944-33336963 MS.gene84188:intron 15.0%
!!! TTATCTTATACGATTTTTTA+TGG - chr7.1:33335858-33335877 MS.gene84188:intron 15.0%
!!! TTATGGATAAAATGTTATTT+TGG - chr7.1:33335875-33335894 MS.gene84188:intron 15.0%
!! AAATGAATTATATCCAATCA+TGG + chr7.1:33335175-33335194 None:intergenic 20.0%
!! AATTTGAATATTGTTTGTCA+GGG - chr7.1:33335496-33335515 MS.gene84188:CDS 20.0%
!! ACAAAAAATTACACTAATAG+AGG + chr7.1:33334982-33335001 None:intergenic 20.0%
!! AGAAAGAAAAGAAAAAAAAC+AGG + chr7.1:33335199-33335218 None:intergenic 20.0%
!! GAAAGAAAAGAAAAAAAACA+GGG + chr7.1:33335198-33335217 None:intergenic 20.0%
!! GTTAATCAAATACTATTACT+TGG + chr7.1:33336497-33336516 None:intergenic 20.0%
!! TAATATTTAGTTAGAGTTGT+AGG - chr7.1:33335427-33335446 MS.gene84188:intron 20.0%
!! TCTCAAAAAAACTATAATCT+CGG - chr7.1:33336173-33336192 MS.gene84188:intron 20.0%
!! TTTAAATAAAAAAGAGAAGG+AGG + chr7.1:33335333-33335352 None:intergenic 20.0%
!! TTTATATAAGTCAATTTGGA+TGG + chr7.1:33336333-33336352 None:intergenic 20.0%
!! TTTGTAATTCATATAACTGT+GGG + chr7.1:33336085-33336104 None:intergenic 20.0%
!!! AAGAAATTTTCATATTTGGA+AGG - chr7.1:33334898-33334917 MS.gene84188:intron 20.0%
!!! ACTTGTATTTCAATTTTCAA+GGG + chr7.1:33336372-33336391 None:intergenic 20.0%
!!! AGAATTGTAGAAAGTTTTAA+AGG - chr7.1:33337122-33337141 MS.gene84188:intron 20.0%
!!! ATTTTGTATTATTTGTTGGA+AGG - chr7.1:33337238-33337257 MS.gene84188:intron 20.0%
!!! GAATTGTAGAAAGTTTTAAA+GGG - chr7.1:33337123-33337142 MS.gene84188:intron 20.0%
!!! GTCTAAGAAATTTTCATATT+TGG - chr7.1:33334894-33334913 MS.gene84188:intron 20.0%
!!! TACTTGTATTTCAATTTTCA+AGG + chr7.1:33336373-33336392 None:intergenic 20.0%
!!! TTATAAACTTGTTTGATCAA+AGG + chr7.1:33336227-33336246 None:intergenic 20.0%
!!! TTTTGTAATTCATATAACTG+TGG + chr7.1:33336086-33336105 None:intergenic 20.0%
! ACAACACATGAAAAATTTGT+AGG - chr7.1:33335821-33335840 MS.gene84188:intron 25.0%
! AGTCGTGAATGAATAATTTA+CGG - chr7.1:33334594-33334613 MS.gene84188:CDS 25.0%
! ATTTGAATATTGTTTGTCAG+GGG - chr7.1:33335497-33335516 MS.gene84188:CDS 25.0%
! CAATTTGAATATTGTTTGTC+AGG - chr7.1:33335495-33335514 MS.gene84188:CDS 25.0%
! CTCAAAAAAACTATAATCTC+GGG - chr7.1:33336174-33336193 MS.gene84188:intron 25.0%
! CTTATTTGAATTGAGATAGA+TGG - chr7.1:33334936-33334955 MS.gene84188:intron 25.0%
! GATTGTTGAATAAGATTTGA+TGG - chr7.1:33335052-33335071 MS.gene84188:intron 25.0%
! GTAAATGTAGAAGACATATT+TGG + chr7.1:33337003-33337022 None:intergenic 25.0%
! TACGATATAGAACAGAAATT+TGG + chr7.1:33336616-33336635 None:intergenic 25.0%
! TTTAATGACTACATCCTATA+GGG + chr7.1:33336805-33336824 None:intergenic 25.0%
!! ATCATGCAAAGTATTATTAG+TGG + chr7.1:33334475-33334494 None:intergenic 25.0%
!! GGATTTGTAAAACTGTTTTA+GGG + chr7.1:33336003-33336022 None:intergenic 25.0%
!! TGGATTTGTAAAACTGTTTT+AGG + chr7.1:33336004-33336023 None:intergenic 25.0%
!! TTTTTCTTAAGTCAAGTCAA+AGG + chr7.1:33335242-33335261 None:intergenic 25.0%
!!! CAATTTTCAAGGGAATTATT+TGG + chr7.1:33336362-33336381 None:intergenic 25.0%
!!! GTTTGGTTTGCTAAAAAATA+AGG + chr7.1:33336059-33336078 None:intergenic 25.0%
!!! TTGTCATCAATTTTGATTGT+CGG + chr7.1:33335145-33335164 None:intergenic 25.0%
AAAATGGTTGAACTATGATC+CGG - chr7.1:33334667-33334686 MS.gene84188:CDS 30.0%
AATGACTAATATGCTCATCT+TGG - chr7.1:33336404-33336423 MS.gene84188:CDS 30.0%
AGACAAAACAGAACAACATA+AGG - chr7.1:33335794-33335813 MS.gene84188:intron 30.0%
ATCTACAAATTGCACTAAAC+AGG + chr7.1:33334500-33334519 None:intergenic 30.0%
CCATGATTTAAAAACCGAAT+TGG - chr7.1:33334555-33334574 MS.gene84188:CDS 30.0%
CTAAACCAAAAACTTACTTG+TGG + chr7.1:33336474-33336493 None:intergenic 30.0%
CTTTAATGACTACATCCTAT+AGG + chr7.1:33336806-33336825 None:intergenic 30.0%
GATTTAAAAACCGAATTGGA+CGG - chr7.1:33334559-33334578 MS.gene84188:CDS 30.0%
TACACTAATAGAGGTTTATG+TGG + chr7.1:33334973-33334992 None:intergenic 30.0%
TATTTAGTTAGAGTTGTAGG+AGG - chr7.1:33335430-33335449 MS.gene84188:intron 30.0%
TTATGACCAATATAAATGGG+TGG + chr7.1:33335614-33335633 None:intergenic 30.0%
TTATTTGAACAGTTTGATCG+TGG - chr7.1:33334622-33334641 MS.gene84188:CDS 30.0%
TTGGATATGTTCAGAAATAC+AGG - chr7.1:33336423-33336442 MS.gene84188:CDS 30.0%
TTTATAGTGTTTGACCCTAT+AGG - chr7.1:33336788-33336807 MS.gene84188:intron 30.0%
! CCAATTCGGTTTTTAAATCA+TGG + chr7.1:33334558-33334577 None:intergenic 30.0%
! GTTTGCTAAAAAATAAGGAC+AGG + chr7.1:33336054-33336073 None:intergenic 30.0%
!! TACCAAAGGGTACTAAAAAA+GGG - chr7.1:33334767-33334786 MS.gene84188:intron 30.0%
!!! ATTTTAAAGTAGAGTTGTCG+AGG + chr7.1:33335090-33335109 None:intergenic 30.0%
!!! GTTTTAGGGATATGAAAAAC+TGG + chr7.1:33335989-33336008 None:intergenic 30.0%
AAAAAACTATAATCTCGGGC+TGG - chr7.1:33336178-33336197 MS.gene84188:intron 35.0%
AAACCAAGCTCTTTGATACT+TGG - chr7.1:33335266-33335285 MS.gene84188:intron 35.0%
AACCAAAAACTTACTTGTGG+TGG + chr7.1:33336471-33336490 None:intergenic 35.0%
AATAAGAAACTAGGCACCAA+AGG + chr7.1:33337587-33337606 None:intergenic 35.0%
ACAACTCCATGATTTCTTTG+TGG - chr7.1:33334408-33334427 MS.gene84188:CDS 35.0%
AGGTATACTGAAAGGAATGA+AGG - chr7.1:33335530-33335549 MS.gene84188:intron 35.0%
ATCTGCAAAACAGTAAGTAC+TGG - chr7.1:33335758-33335777 MS.gene84188:intron 35.0%
ATTCCTTTCAGTATACCTCT+TGG + chr7.1:33335528-33335547 None:intergenic 35.0%
CAAGAAAAACCTCAGTTGTA+CGG + chr7.1:33336030-33336049 None:intergenic 35.0%
CCAGATTCAAACGGATAAAA+TGG - chr7.1:33334651-33334670 MS.gene84188:CDS 35.0%
GAACATGTATAACTTCCAAG+AGG - chr7.1:33337180-33337199 MS.gene84188:intron 35.0%
TAAGGAAGTTCTTCAAATGC+AGG - chr7.1:33336726-33336745 MS.gene84188:intron 35.0%
TACTTTGTCCAATTCTGTCT+AGG + chr7.1:33337307-33337326 None:intergenic 35.0%
TCAAACAGCAAGAAAGCAAA+AGG - chr7.1:33337442-33337461 MS.gene84188:intron 35.0%
TCTTCTCCACCCATTTATAT+TGG - chr7.1:33335605-33335624 MS.gene84188:intron 35.0%
TGACAACGTAAATCCATGAT+TGG - chr7.1:33335159-33335178 MS.gene84188:intron 35.0%
TGGTTATGTCCAGATTCAAA+CGG - chr7.1:33334642-33334661 MS.gene84188:CDS 35.0%
TGTTCCTTGATCATCTATGA+AGG - chr7.1:33336553-33336572 MS.gene84188:intron 35.0%
TTAGGGATATGAAAAACTGG+TGG + chr7.1:33335986-33336005 None:intergenic 35.0%
TTATTCATTCACGACTCAAC+TGG + chr7.1:33334590-33334609 None:intergenic 35.0%
! ATCCACCACAAGTAAGTTTT+TGG - chr7.1:33336466-33336485 MS.gene84188:intron 35.0%
! ATTTTAGGCAACTTGTACGT+GGG - chr7.1:33335899-33335918 MS.gene84188:intron 35.0%
! CCATTTTATCCGTTTGAATC+TGG + chr7.1:33334654-33334673 None:intergenic 35.0%
!! ATCCTTTAAAACACAATCCG+AGG - chr7.1:33337412-33337431 MS.gene84188:intron 35.0%
!! CTACCAAAGGGTACTAAAAA+AGG - chr7.1:33334766-33334785 MS.gene84188:intron 35.0%
!! TATTTTAGGCAACTTGTACG+TGG - chr7.1:33335898-33335917 MS.gene84188:intron 35.0%
!! TTCCTCGGATTGTGTTTTAA+AGG + chr7.1:33337417-33337436 None:intergenic 35.0%
!! TTTTAGTACCCTTTGGTAGT+AGG + chr7.1:33334765-33334784 None:intergenic 35.0%
!!! AACCCTTTTTTAGTACCCTT+TGG + chr7.1:33334772-33334791 None:intergenic 35.0%
!!! TTTCCTACAGGTTTTGAACT+AGG - chr7.1:33337482-33337501 MS.gene84188:intron 35.0%
AAGAAAAGCTTGCAAGCCTT+TGG - chr7.1:33337346-33337365 MS.gene84188:intron 40.0%
ACGCATGAGTGATAAAGTTG+AGG - chr7.1:33336762-33336781 MS.gene84188:intron 40.0%
ACTCCATGATTTCTTTGTGG+AGG - chr7.1:33334411-33334430 MS.gene84188:CDS 40.0%
ACTGAAAGGAATGAAGGCTA+TGG - chr7.1:33335536-33335555 MS.gene84188:intron 40.0%
AGGCTGAAAATTGAAGCACA+AGG - chr7.1:33337275-33337294 MS.gene84188:intron 40.0%
AGTCCAAGTATCAAAGAGCT+TGG + chr7.1:33335272-33335291 None:intergenic 40.0%
AGTGGCAAAAACTCACTATC+TGG + chr7.1:33334457-33334476 None:intergenic 40.0%
ATGCTCAATCCTACTACCAA+AGG - chr7.1:33334753-33334772 MS.gene84188:intron 40.0%
CAAAAAGTTGTGTGGTGGTT+TGG - chr7.1:33335923-33335942 MS.gene84188:intron 40.0%
CACACAAAGAACGCTTAAGA+TGG - chr7.1:33334380-33334399 MS.gene84188:CDS 40.0%
CTGAAAGGAATGAAGGCTAT+GGG - chr7.1:33335537-33335556 MS.gene84188:intron 40.0%
GAATAGTGCTCTTCAGCTTA+AGG - chr7.1:33336708-33336727 MS.gene84188:intron 40.0%
GATCATCTATGAAGGAGGAA+AGG - chr7.1:33336561-33336580 MS.gene84188:intron 40.0%
GATCCTAGTTCAAAACCTGT+AGG + chr7.1:33337488-33337507 None:intergenic 40.0%
GGAAGGTTCAAAAAAGCTTG+AGG - chr7.1:33337255-33337274 MS.gene84188:intron 40.0%
TCAGATTCCTCAGTAAGAGA+GGG + chr7.1:33337392-33337411 None:intergenic 40.0%
TCCTCCTTCATAGATGATCA+AGG + chr7.1:33336560-33336579 None:intergenic 40.0%
TCCTTGATCATCTATGAAGG+AGG - chr7.1:33336556-33336575 MS.gene84188:intron 40.0%
TCTTAAGCGTTCTTTGTGTG+TGG + chr7.1:33334380-33334399 None:intergenic 40.0%
TCTTTGTGGAGGCTGTAAAT+AGG - chr7.1:33334422-33334441 MS.gene84188:CDS 40.0%
TGAAAGGAATGAAGGCTATG+GGG - chr7.1:33335538-33335557 MS.gene84188:intron 40.0%
TGCTCAATCCTACTACCAAA+GGG - chr7.1:33334754-33334773 MS.gene84188:intron 40.0%
TTCAGATTCCTCAGTAAGAG+AGG + chr7.1:33337393-33337412 None:intergenic 40.0%
! ACACCAAGAGGTATACTGAA+AGG - chr7.1:33335522-33335541 MS.gene84188:intron 40.0%
! TGCTGTTTGATGTTCTTCCT+CGG + chr7.1:33337432-33337451 None:intergenic 40.0%
!! AACTTACTTGTGGTGGATTC+TGG + chr7.1:33336464-33336483 None:intergenic 40.0%
!! GGAAAGTACCTAGACAGAAT+TGG - chr7.1:33337296-33337315 MS.gene84188:intron 40.0%
!! GGTGGATTCTGGAATCAATT+TGG + chr7.1:33336453-33336472 None:intergenic 40.0%
!!! GATGAGAGTGTTTTTCCTAC+AGG - chr7.1:33337470-33337489 MS.gene84188:intron 40.0%
AAACCTCAGTTGTACGGTGT+TGG + chr7.1:33336024-33336043 None:intergenic 45.0%
AAAGCTTGCAAGCCTTTGGT+TGG - chr7.1:33337350-33337369 MS.gene84188:intron 45.0%
AACTAGGCACCAAAGGTGAT+TGG + chr7.1:33337580-33337599 None:intergenic 45.0%
AATCCAACACCGTACAACTG+AGG - chr7.1:33336018-33336037 MS.gene84188:intron 45.0%
ACCAAACCTGGAACCTTTCT+TGG - chr7.1:33337531-33337550 MS.gene84188:CDS 45.0%
ACGTGGGACAAAAAGTTGTG+TGG - chr7.1:33335915-33335934 MS.gene84188:intron 45.0%
ACTGGTTGAACCGTCCAATT+CGG + chr7.1:33334572-33334591 None:intergenic 45.0%
AGCAACCTACCAATCACCTT+TGG - chr7.1:33337568-33337587 MS.gene84188:CDS 45.0%
AGTTCTTCAAATGCAGGCAG+AGG - chr7.1:33336732-33336751 MS.gene84188:intron 45.0%
CAGAATTCAGCAACCAAACC+TGG - chr7.1:33337519-33337538 MS.gene84188:CDS 45.0%
CAGCCTCCACAAAGAAATCA+TGG + chr7.1:33334417-33334436 None:intergenic 45.0%
CATATAACTGTGGGTGCGTT+TGG + chr7.1:33336076-33336095 None:intergenic 45.0%
CATTGAGCCTACAAGACATC+CGG + chr7.1:33334689-33334708 None:intergenic 45.0%
CCAAGAGGACAAAACAGACA+TGG - chr7.1:33337195-33337214 MS.gene84188:intron 45.0%
CTATGATCCGGATGTCTTGT+AGG - chr7.1:33334679-33334698 MS.gene84188:CDS 45.0%
GAAAGGAATGAAGGCTATGG+GGG - chr7.1:33335539-33335558 MS.gene84188:intron 45.0%
TCCAAGAAAGGTTCCAGGTT+TGG + chr7.1:33337535-33337554 None:intergenic 45.0%
TGGAGGCTGTAAATAGGCTT+GGG - chr7.1:33334428-33334447 MS.gene84188:CDS 45.0%
TGGGACAAAAAGTTGTGTGG+TGG - chr7.1:33335918-33335937 MS.gene84188:intron 45.0%
TGTCTTGTAGGCTCAATGTC+TGG - chr7.1:33334691-33334710 MS.gene84188:intron 45.0%
! CCATGTCTGTTTTGTCCTCT+TGG + chr7.1:33337198-33337217 None:intergenic 45.0%
! GGTTTGGTTGCTGAATTCTG+AGG + chr7.1:33337519-33337538 None:intergenic 45.0%
!! GGTGTTGCTTTACCAACCAA+AGG + chr7.1:33337365-33337384 None:intergenic 45.0%
!! GTTGCTGAATTCTGAGGTAG+TGG + chr7.1:33337513-33337532 None:intergenic 45.0%
ACACGTCAAAAGCCACTTGC+AGG - chr7.1:33335581-33335600 MS.gene84188:intron 50.0%
ACCTCTTCCCTCTCTTACTG+AGG - chr7.1:33337382-33337401 MS.gene84188:intron 50.0%
AGGCACCAAAGGTGATTGGT+AGG + chr7.1:33337576-33337595 None:intergenic 50.0%
AGGCTGTAAATAGGCTTGGG+GGG - chr7.1:33334431-33334450 MS.gene84188:CDS 50.0%
CTGACTCCAAGAAAGGTTCC+AGG + chr7.1:33337540-33337559 None:intergenic 50.0%
GAACCTTTCTTGGAGTCAGC+TGG - chr7.1:33337541-33337560 MS.gene84188:CDS 50.0%
GAGGCTGTAAATAGGCTTGG+GGG - chr7.1:33334430-33334449 MS.gene84188:CDS 50.0%
GGAGGCTGTAAATAGGCTTG+GGG - chr7.1:33334429-33334448 MS.gene84188:CDS 50.0%
GTGGAGGCTGTAAATAGGCT+TGG - chr7.1:33334427-33334446 MS.gene84188:CDS 50.0%
TCCTCAGTAAGAGAGGGAAG+AGG + chr7.1:33337386-33337405 None:intergenic 50.0%
!! AGTTGTGTGGTGGTTTGGTG+AGG - chr7.1:33335928-33335947 MS.gene84188:intron 50.0%
!! GTTGTGTGGTGGTTTGGTGA+GGG - chr7.1:33335929-33335948 MS.gene84188:intron 50.0%
ATGGGTTCCACTCGCTCAGA+TGG - chr7.1:33334349-33334368 MS.gene84188:CDS 55.0%
GGTGGAGAAGAACCTGCAAG+TGG + chr7.1:33335596-33335615 None:intergenic 55.0%
TTGTCAGGGGCAACACCAAG+AGG - chr7.1:33335510-33335529 MS.gene84188:intron 55.0%
! CAGCCAGCTGACTCCAAGAA+AGG + chr7.1:33337547-33337566 None:intergenic 55.0%
GGCAGAGCCATCTGAGCGAG+TGG + chr7.1:33334359-33334378 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 33334349 33337610 33334349 ID=MS.gene84188
chr7.1 mRNA 33334349 33337610 33334349 ID=MS.gene84188.t1;Parent=MS.gene84188
chr7.1 exon 33337499 33337610 33337499 ID=MS.gene84188.t1.exon1;Parent=MS.gene84188.t1
chr7.1 CDS 33337499 33337610 33337499 ID=cds.MS.gene84188.t1;Parent=MS.gene84188.t1
chr7.1 exon 33336357 33336442 33336357 ID=MS.gene84188.t1.exon2;Parent=MS.gene84188.t1
chr7.1 CDS 33336357 33336442 33336357 ID=cds.MS.gene84188.t1;Parent=MS.gene84188.t1
chr7.1 exon 33335483 33335522 33335483 ID=MS.gene84188.t1.exon3;Parent=MS.gene84188.t1
chr7.1 CDS 33335483 33335522 33335483 ID=cds.MS.gene84188.t1;Parent=MS.gene84188.t1
chr7.1 exon 33335327 33335384 33335327 ID=MS.gene84188.t1.exon4;Parent=MS.gene84188.t1
chr7.1 CDS 33335327 33335384 33335327 ID=cds.MS.gene84188.t1;Parent=MS.gene84188.t1
chr7.1 exon 33335176 33335245 33335176 ID=MS.gene84188.t1.exon5;Parent=MS.gene84188.t1
chr7.1 CDS 33335176 33335245 33335176 ID=cds.MS.gene84188.t1;Parent=MS.gene84188.t1
chr7.1 exon 33334349 33334699 33334349 ID=MS.gene84188.t1.exon6;Parent=MS.gene84188.t1
chr7.1 CDS 33334349 33334699 33334349 ID=cds.MS.gene84188.t1;Parent=MS.gene84188.t1
Gene Sequence

>MS.gene84188

ATGGGTTCCACTCGCTCAGATGGCTCTGCCACACACAAAGAACGCTTAAGATGGACACAACAACTCCATGATTTCTTTGTGGAGGCTGTAAATAGGCTTGGGGGGCCAGATAGTGAGTTTTTGCCACTAATAATACTTTGCATGATAACCTGTTTAGTGCAATTTGTAGATTTTTTGTTAACGTGCTAAGTTTCTTTAGTTCACAGCCATGATTTAAAAACCGAATTGGACGGTTCAACCAGTTGAGTCGTGAATGAATAATTTACGGTTTGATTATTTGAACAGTTTGATCGTGGTTATGTCCAGATTCAAACGGATAAAATGGTTGAACTATGATCCGGATGTCTTGTAGGCTCAATGTCTGGTTCAGTTTTTAAATTATTAGTTCAAAGGAAATATGTCAAATGCTCAATCCTACTACCAAAGGGTACTAAAAAAGGGTTAATGAAGAGATGTTTGTTGATTTGTGTTTGCAATATTTACAATGATAGAATTTATGATTTCTTCAAAAAAAAAAAATGATAGAATTTATGAACTTTTTCTTTGTCTAAGAAATTTTCATATTTGGAAGGCAACTTAAAAGTTCACTTATTTGAATTGAGATAGATGGACTTACACAAACCACATAAACCTCTATTAGTGTAATTTTTTGTACTCAAATTGTTGCTATTAAGATAATCATGTTAAGCAGTCAAGAGATCTTGATTGTTGAATAAGATTTGATGGCTCATATTCTGACCTCGACAACTCTACTTTAAAATGAAACTTGAAATGTAAGTTGTCTGATTTTCATCCGACAATCAAAATTGATGACAACGTAAATCCATGATTGGATATAATTCATTTCCCTGTTTTTTTTCTTTTCTTTCTTTCTATTCACATGACTCAAACCTTTGACTTGACTTAAGAAAAATTAAAAACCAAGCTCTTTGATACTTGGACTAACAATTTGATTTATAAACTTATTTGTAGTTGCATTTTCCTCCTTCTCTTTTTTATTTAAAAATGATTTTGTGTGCTACTGATATATCTATTTTGACTTTTTTACGTTAACTTTTAGCTTGAAAGTATAATATGATAATATTTAGTTAGAGTTGTAGGAGGCAACATTATTTACATAGAAAAGTTTATGATTATATGCATTTTCAATTTGAATATTGTTTGTCAGGGGCAACACCAAGAGGTATACTGAAAGGAATGAAGGCTATGGGGGTTTCAGAATTGAAAATATTACACGTCAAAAGCCACTTGCAGGTTCTTCTCCACCCATTTATATTGGTCATAAAATTTTGAATGAATTTATCACATGATCTAAAGTATTAAGTATAAACACTTGTACAAGTATTTAGAGTAGTTTATGAAAATAAATTATAATATGACTTTTTTTTTTATTATATAAACTTAGTTTTATCTGCAAAACAGTAAGTACTGGACAAAACATTACAAGACAAAACAGAACAACATAAGGTAGAACAACACATGAAAAATTTGTAGGTATTGAATAATTTTTTATCTTATACGATTTTTTATGGATAAAATGTTATTTTGGTATTTTAGGCAACTTGTACGTGGGACAAAAAGTTGTGTGGTGGTTTGGTGAGGGACAAGAATTTCAATTTTTGTCTTGTCGCTTGCCACCAGTTTTTCATATCCCTAAAACAGTTTTACAAATCCAACACCGTACAACTGAGGTTTTTCTTGTCCTGTCCTTATTTTTTAGCAAACCAAACGCACCCACAGTTATATGAATTACAAAATATGCTAATTAGTAAAAATGGAAGAAAATATTAAAAGCTACAAGTTCAAACTGTATTTAAAATAGCTTCTCAAAAAAACTATAATCTCGGGCTGGAAGCTTGTTTATCAAACACATTTCCTTTGATCAAACAAGTTTATAAGTTATTTCAATAAGCTAAAAGTTGTTTGTACAAACTCACTGCATGAAATTATTTATTAGTGTTTTGACACATTTATACTTTTACCATCCAAATTGACTTATATAAAGTAAAGCCAAATAATTCCCTTGAAAATTGAAATACAAGTAACGTTATAAACAATGACTAATATGCTCATCTTGGATATGTTCAGAAATACAGGATTTCCAAATTGATTCCAGAATCCACCACAAGTAAGTTTTTGGTTTAGCCAAGTAATAGTATTTGATTAACTAATGATATATACCAAATTCAATTGAAATTATAACTTGTTCCTTGATCATCTATGAAGGAGGAAAGGTTGAGAAGAGAAGCATATCAGATATACTGCCAAATTTCTGTTCTATATCGTAAGTCAACAACTAATTAAGCAATAATTTTCATATCTCATTTTCAAACAACTTATATAATTATATTTTGAAAATGAATAGTGCTCTTCAGCTTAAGGAAGTTCTTCAAATGCAGGCAGAGGTGCAAAAACGCATGAGTGATAAAGTTGAGGTATTTTATAGTGTTTGACCCTATAGGATGTAGTCATTAAAGTTTGATTTATCTAATTTTCTTTTTAGTAAACTTTCACACAAAAATTTAACAGTTTGTTGAAGAAAAATAACTTAAAAGTTTCAATATTATGTTTTTTTTTTTTTTTATCAAGTAAGCATATGATATTTTAATTTGTCAAGGAAGATCAAACATATTACACTTTTGCTTCTTTATCCAAATATGTCTTCTACATTTACAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAGTCTTTATAATTTTTAGGTCAAGAATTGTAGAAAGTTTTAAAGGGAATTAAAACAAAACAATATAATTATTTCTATGGAGAACATGTATAACTTCCAAGAGGACAAAACAGACATGGAGATAATCTCTAAAGTCAAAATTTTGTATTATTTGTTGGAAGGTTCAAAAAAGCTTGAGGCTGAAAATTGAAGCACAAGGAAAGTACCTAGACAGAATTGGACAAAGTAATCAAATCAGAACTATTACAAGAAAAGCTTGCAAGCCTTTGGTTGGTAAAGCAACACCTCTTCCCTCTCTTACTGAGGAATCTGAATCCTTTAAAACACAATCCGAGGAAGAACATCAAACAGCAAGAAAGCAAAAGGTCACAGATGAGAGTGTTTTTCCTACAGGTTTTGAACTAGGATCATCCACTACCTCAGAATTCAGCAACCAAACCTGGAACCTTTCTTGGAGTCAGCTGGCTGAAGCAACCTACCAATCACCTTTGGTGCCTAGTTTCTTATTATAG
Protein sequence

>MS.gene84188.t1

MGSTRSDGSATHKERLRWTQQLHDFFVEAVNRLGGPDRATPRGILKGMKAMGVSELKILHVKSHLQKYRISKLIPESTTRGKVEKRSISDILPNFCSISALQLKEVLQMQAEVQKRMSDKVEVQKSLRLKIEAQGKYLDRIGQSNQIRTITRKACKPLVGKATPLPSLTEESESFKTQSEEEHQTARKQKVTDESVFPTGFELGSSTTSEFSNQTWNLSWSQLAEATYQSPLVPSFLL