AlfalfaGEDB | MS.gene84311

M. sativa cultivar XinJiangDaYe / MS.gene84311

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84311.t1	XP_003596841.1	88.6	254	24	4	1	254	1	249	3.40E-126	461.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84311.t1	Q9FMR3	53.6	250	90	5	7	253	8	234	5.8e-66	252.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84311.t1	G7IHF4	88.6	254	24	4	1	254	1	249	2.5e-126	461.1

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene84311.t1	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84311.t1	MTR_2g086690	88.189	254	25	4	1	254	1	249	8.72e-163	451
MS.gene84311.t1	MTR_4g052620	60.150	266	87	8	1	253	1	260	1.28e-103	301
MS.gene84311.t1	MTR_7g105170	46.591	264	119	6	1	254	1	252	9.93e-77	233
MS.gene84311.t1	MTR_4g134460	44.099	161	75	4	6	164	56	203	5.46e-38	138
MS.gene84311.t1	MTR_4g075980	42.529	174	80	6	6	175	17	174	3.02e-37	133
MS.gene84311.t1	MTR_6g011860	41.379	174	88	6	1	167	12	178	1.03e-36	134
MS.gene84311.t1	MTR_5g041940	45.223	157	78	5	5	160	4	153	1.59e-36	130
MS.gene84311.t1	MTR_6g477900	36.275	204	118	5	5	206	7	200	1.67e-36	129
MS.gene84311.t1	MTR_2g079990	46.203	158	71	5	5	160	17	162	4.24e-36	131
MS.gene84311.t1	MTR_5g021710	42.246	187	93	7	5	188	9	183	5.08e-36	131

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84311.t1	AT5G22380	53.600	250	90	5	7	253	8	234	2.72e-84	251
MS.gene84311.t1	AT3G44350	50.794	252	110	5	2	253	3	240	5.52e-82	246
MS.gene84311.t1	AT3G44350	62.238	143	53	1	2	144	3	144	1.64e-60	188
MS.gene84311.t1	AT4G17980	46.835	158	74	6	5	159	7	157	3.65e-39	137
MS.gene84311.t1	AT4G17980	46.835	158	74	6	5	159	7	157	6.21e-39	137
MS.gene84311.t1	AT2G02450	43.949	157	73	4	6	160	53	196	1.87e-38	138
MS.gene84311.t1	AT2G02450	43.949	157	73	4	6	160	53	196	2.61e-38	138
MS.gene84311.t1	AT3G15500	47.468	158	69	5	5	160	15	160	6.39e-38	135
MS.gene84311.t1	AT3G17730	38.144	194	102	6	5	196	7	184	2.30e-37	132
MS.gene84311.t1	AT2G17040	41.520	171	80	6	6	172	8	162	2.86e-37	132

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Find 80 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTATCAACTTCCCACT+CGG	0.573290	2.2:-12011553	MS.gene84311:CDS
AACAAGTGCTCATCAACAAG+TGG	0.605093	2.2:-12011595	MS.gene84311:CDS
AACTTATCAACTTCCCACTC+GGG	0.587994	2.2:-12011552	MS.gene84311:CDS
AATTGTGTTACGTACTTGGA+AGG	0.510602	2.2:+12013082	None:intergenic
ACAACAAAGTCATTGGAATA+AGG	0.339902	2.2:-12012280	MS.gene84311:CDS
ACCATTAGAGGGGCCAAAGA+CGG	0.556738	2.2:-12011643	MS.gene84311:CDS
ACTTCCCACTCGGGAGAGGT+AGG	0.537886	2.2:-12011543	MS.gene84311:CDS
AGACAAGAGAGAGAGTTGAG+TGG	0.675775	2.2:-12012377	MS.gene84311:CDS
AGGGTTTGTATTGGATGGGT+CGG	0.541150	2.2:+12012172	None:intergenic
AGTTGTTCCCATTCCCAAAG+AGG	0.523806	2.2:+12011429	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAACAAAGTCATTGGAATA+AGG	-	chr2.2:12012367-12012386	MS.gene84311:CDS	30.0%
ATACCTGTCATTGATATCAA+TGG	-	chr2.2:12011527-12011546	MS.gene84311:CDS	30.0%
ATATTCATGCATCTTCCATT+TGG	+	chr2.2:12012439-12012458	None:intergenic	30.0%
GAATGATGTACCAAATAATG+GGG	-	chr2.2:12013178-12013197	MS.gene84311:CDS	30.0%
GGAATGATGTACCAAATAAT+GGG	-	chr2.2:12013177-12013196	MS.gene84311:CDS	30.0%
GTGACATTATTTGAGAATCA+CGG	+	chr2.2:12012611-12012630	None:intergenic	30.0%
TCTTCTAACAACAAAGTCAT+TGG	-	chr2.2:12012360-12012379	MS.gene84311:CDS	30.0%
TGGAATGATGTACCAAATAA+TGG	-	chr2.2:12013176-12013195	MS.gene84311:CDS	30.0%
AAACTTATCAACTTCCCACT+CGG	-	chr2.2:12013094-12013113	MS.gene84311:intron	35.0%
AACATAACCACGATCCAAAA+AGG	+	chr2.2:12012585-12012604	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	12011416	12013253	12011416	ID=MS.gene84311
chr2.2	mRNA	12011416	12013253	12011416	ID=MS.gene84311.t1;Parent=MS.gene84311
chr2.2	exon	12013097	12013253	12013097	ID=MS.gene84311.t1.exon1;Parent=MS.gene84311.t1
chr2.2	CDS	12013097	12013253	12013097	ID=cds.MS.gene84311.t1;Parent=MS.gene84311.t1
chr2.2	exon	12012166	12012455	12012166	ID=MS.gene84311.t1.exon2;Parent=MS.gene84311.t1
chr2.2	CDS	12012166	12012455	12012166	ID=cds.MS.gene84311.t1;Parent=MS.gene84311.t1
chr2.2	exon	12011416	12011733	12011416	ID=MS.gene84311.t1.exon3;Parent=MS.gene84311.t1
chr2.2	CDS	12011416	12011733	12011416	ID=cds.MS.gene84311.t1;Parent=MS.gene84311.t1

Gene Sequence

>MS.gene84311

ATGGAGGATTCCCCCCCCGGTTTTCGTTTCTACCCAACAGAAGAAGAGCTAGTTGGCTTCTATCTACACAACCAGCTGGAAGGACAAAAGCATGAAGACATAAACAAAATCATACCTGTCATTGATATCAATGGCAAAGAGCCATGGAACCTTCCAAGTACGTAACACAATTTTTTTACGTAACTCATTCATTTCTTCATGATATATCAATCACTTTTATATAACTTTGATTGTCTCAAAATAATTCATTCAAAATATACTTCAATATCACAACACTAGAAATATAAAATTTTCCATGAAGAAAAATTTTATCTCAAATTCTCTAGTTACTTAATTTAGTGATAGAATATTATAAATTCCTTCCTCCTTCAAAAAAAAAAAAAAAAGAATATTATAAACGGATTTTATAGTTGACATGCATACTCTATCTATAAGTAGTGTCATGATAAGTTAATATTTTTCATATTCTGTCAGAAAAAAGAATATTTTTCATAGATACGATACAATATTTATTCTTTTTATATACAAATAATTATTTCGGCTATGATTTCCCTCCAATCTCATATTTAAGAAAAGATTTTTTTTTTTAGGTTCATTGAATAATTAATGTATCTAGTCTACATCGTAAAACAGATCAATTAGTTATTCAATAAACCTAAAAAAGAAAATTATATAAGACAGGAGGGAGTGCATGTCTAAGATTGACACCGTCATTCATGTAGATATTTACTAAGATATTATAACACATGTGAGAGTAATTAAGATTGATGACAATGTAAATATCTTGCTTGCAATCAGCATTTGCAGGGGAGAGATGTCGTGGAGACACAGAGCAATGGTTTTTCTTTTCACCAAGACAAGAGAGAGAGTTGAGTGGTGGAAGACCAAACAGAACAACATCTTGTGGGTATTGGAAAGCTACGGGTTCTCCGAGCTACGTTTACTCTTCTAACAACAAAGTCATTGGAATAAGGAAAACCATGGTGTTTTACATAGGAAAAGCTCCATCTGGAAGGAAAACCAAATGGAAGATGCATGAATATAAAGCCATTGAACCATCCGACCCATCCAATACAAACCCTCCTACGGTATATTTGAAAAAGTAGAACTCATCAGCATGATTAATATTAATATTAATCATGCTAACAAGTTCTTATTTTTCCCTTCCTTTTTGGATCGTGGTTATGTTTTACCGTGATTCTCAAATAATGTCACATATTGTAGTGTGATTGCACCTTAAAATATTTATCATACATTTATAAACTTAAAAAGAGATATGCGACAACAATCAAGATTGTGACATCATAATTTCGATTTTTTTTTGTGCCTACAATGCTACTAAATGCGATTAAAGTCATATTGATCATATTTGAATGTGATTTTATACAATATACAATAAATTTTGACGCGGTCTTAATATGATCACGATATAAAATCGTAGTCATAAATTCACATGTTATCATAAGCTTAATTTTTTTTATTTTTTTAATTGATCAATAATTTATTTTGGTGTGTTGTAGCTGCGACACGAAATCAGCTTGTGTCGCGTTTATGTGATATCGGGAAGCTTTAGTTCATTTGATCGAAGACCATTAGAGGGGCCAAAGACGGAGTTACAACTTGATCATGTTGCTTCAACAAGTGCTCATCAACAAGTGGTAGCAGAGCTCGATGGATCAAACTTATCAACTTCCCACTCGGGAGAGGTAGGGAGAGACCCTGATGCTGCTCTTAATATTGGAGGGTCAAGTGGCACGAATTGGAATGATGTACCAAATAATGGGGTTGATGAACCTGACGAGCCTCTTTGGGAATGGGAACAACTAAATTGGATCTAA

Protein sequence

>MS.gene84311.t1

MEDSPPGFRFYPTEEELVGFYLHNQLEGQKHEDINKIIPVIDINGKEPWNLPTFAGERCRGDTEQWFFFSPRQERELSGGRPNRTTSCGYWKATGSPSYVYSSNNKVIGIRKTMVFYIGKAPSGRKTKWKMHEYKAIEPSDPSNTNPPTLRHEISLCRVYVISGSFSSFDRRPLEGPKTELQLDHVASTSAHQQVVAELDGSNLSTSHSGEVGRDPDAALNIGGSSGTNWNDVPNNGVDEPDEPLWEWEQLNWI