Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84349.t1 | XP_013459206.1 | 79.2 | 269 | 50 | 1 | 1 | 263 | 1 | 269 | 1.80E-98 | 369 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84349.t1 | Q9M202 | 26.4 | 269 | 148 | 11 | 8 | 254 | 6 | 246 | 1.0e-09 | 65.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84349.t1 | A0A072V3Z9 | 79.2 | 269 | 50 | 1 | 1 | 263 | 1 | 269 | 1.3e-98 | 369.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene84349.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84349.t1 | MTR_3g408380 | 90.706 | 269 | 19 | 1 | 1 | 263 | 1 | 269 | 0.0 | 503 |
| MS.gene84349.t1 | MTR_3g008310 | 83.636 | 275 | 33 | 1 | 1 | 263 | 1 | 275 | 1.15e-168 | 467 |
| MS.gene84349.t1 | MTR_3g408420 | 64.130 | 276 | 85 | 3 | 1 | 263 | 1 | 275 | 5.86e-113 | 326 |
| MS.gene84349.t1 | MTR_0160s0030 | 26.797 | 306 | 164 | 10 | 8 | 255 | 6 | 309 | 6.67e-14 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84349.t1 | AT3G60580 | 26.394 | 269 | 148 | 10 | 8 | 254 | 6 | 246 | 2.10e-13 | 68.9 |
| MS.gene84349.t1 | AT1G02030 | 26.482 | 253 | 152 | 8 | 8 | 254 | 7 | 231 | 1.76e-12 | 66.2 |
Find 45 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAATGAATGGAAACAAAT+TGG | 0.206002 | 3.1:+3958842 | MS.gene84349:CDS |
| TTGTTTGGTGGGTTACTTCT+TGG | 0.249003 | 3.1:-3958569 | None:intergenic |
| CAGGTACTTGGTTGTTGATT+GGG | 0.275325 | 3.1:-3958478 | None:intergenic |
| TCAGGTACTTGGTTGTTGAT+TGG | 0.290946 | 3.1:-3958479 | None:intergenic |
| CTTCTTGGATTAACAATTGA+TGG | 0.303146 | 3.1:-3958554 | None:intergenic |
| GCCAACTCAGCTGAGTATTC+AGG | 0.304238 | 3.1:-3958497 | None:intergenic |
| TGCTTTGACATCTTTGAATC+TGG | 0.309104 | 3.1:+3959073 | MS.gene84349:CDS |
| TTCAAAGATTGAGGATTGTT+TGG | 0.332480 | 3.1:-3958584 | None:intergenic |
| AGAATGAATGGAAACAAATT+GGG | 0.366606 | 3.1:+3958843 | MS.gene84349:CDS |
| CTTTGAATCTGGTCACGTAT+TGG | 0.390852 | 3.1:+3959084 | MS.gene84349:CDS |
| ACAATCAGCGTATAGCGATT+TGG | 0.398813 | 3.1:+3958784 | MS.gene84349:CDS |
| CAGCTGAGTATTCAGGTACT+TGG | 0.400249 | 3.1:-3958490 | None:intergenic |
| GAGTGTGTCTCATTTGTAAC+AGG | 0.418825 | 3.1:+3958381 | MS.gene84349:CDS |
| AGGTACTTGGTTGTTGATTG+GGG | 0.434003 | 3.1:-3958477 | None:intergenic |
| CAGAATCATAATAATTGTAT+AGG | 0.437191 | 3.1:-3959126 | None:intergenic |
| GTGTTATTCTTAAGAATGAA+TGG | 0.439493 | 3.1:+3958831 | MS.gene84349:CDS |
| AATTTCACTTTCAAAGATTG+AGG | 0.444853 | 3.1:-3958593 | None:intergenic |
| AAATGTACCCCGTCAAACTT+TGG | 0.446972 | 3.1:+3958617 | MS.gene84349:CDS |
| GATAGAGTGAGTTGGATGTT+TGG | 0.448812 | 3.1:-3958519 | None:intergenic |
| AACAATCTAGCAGGAGAGAT+TGG | 0.456742 | 3.1:+3958995 | MS.gene84349:CDS |
| GGATTGGTAGTTTGGCATAG+TGG | 0.460724 | 3.1:-3958445 | None:intergenic |
| TCTTATCCAACAAACCCAAC+TGG | 0.466226 | 3.1:+3958659 | MS.gene84349:CDS |
| TATTCCTACCAAAGTTTGAC+GGG | 0.471579 | 3.1:-3958625 | None:intergenic |
| AAGATTGAGGATTGTTTGGT+GGG | 0.486813 | 3.1:-3958580 | None:intergenic |
| TCAGCCATATGAAACTGTCT+TGG | 0.489788 | 3.1:-3958701 | None:intergenic |
| CTATTCCTACCAAAGTTTGA+CGG | 0.495952 | 3.1:-3958626 | None:intergenic |
| GATGTAGAGATAGAGTGAGT+TGG | 0.497788 | 3.1:-3958527 | None:intergenic |
| TGAATCTGGTCACGTATTGG+AGG | 0.509112 | 3.1:+3959087 | MS.gene84349:CDS |
| AAAGATTGAGGATTGTTTGG+TGG | 0.514150 | 3.1:-3958581 | None:intergenic |
| GATTGGTAGTTTGGCATAGT+GGG | 0.520357 | 3.1:-3958444 | None:intergenic |
| CAAACCAAGACAGTTTCATA+TGG | 0.521057 | 3.1:+3958697 | MS.gene84349:CDS |
| GCAGGAGAGATTGGTAGAAG+TGG | 0.524466 | 3.1:+3959004 | MS.gene84349:CDS |
| AGGAAAACTAACAATCTAGC+AGG | 0.531732 | 3.1:+3958986 | MS.gene84349:CDS |
| ACCTGAATACTCAGCTGAGT+TGG | 0.539000 | 3.1:+3958496 | MS.gene84349:CDS |
| CGTATTGGAGGAACACATGA+AGG | 0.564625 | 3.1:+3959099 | MS.gene84349:CDS |
| GGTACTTGGTTGTTGATTGG+GGG | 0.573211 | 3.1:-3958476 | None:intergenic |
| TCCAATGGAAAAGCTTTGGG+AGG | 0.590411 | 3.1:+3958410 | MS.gene84349:CDS |
| TCGGACATGAAGATATCCAT+AGG | 0.592226 | 3.1:+3958966 | MS.gene84349:CDS |
| ATAGAAGCTGCTGAAACACT+AGG | 0.609115 | 3.1:+3958809 | MS.gene84349:CDS |
| GAATGAATGGAAACAAATTG+GGG | 0.620519 | 3.1:+3958844 | MS.gene84349:CDS |
| GTACCCCGTCAAACTTTGGT+AGG | 0.631191 | 3.1:+3958621 | MS.gene84349:CDS |
| ACAAATATCACACTCAAACA+CGG | 0.640237 | 3.1:-3958912 | None:intergenic |
| TCTCCAACTATGGAGAACAA+TGG | 0.649885 | 3.1:+3958356 | None:intergenic |
| AGAGAATATAGAATTTAACA+TGG | 0.705093 | 3.1:+3958733 | MS.gene84349:CDS |
| ATTCCTACCAAAGTTTGACG+GGG | 0.741422 | 3.1:-3958624 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAGAATATAGAATTTAACA+TGG | + | chr3.1:3958733-3958752 | MS.gene84349:CDS | 20.0% |
| !! | CAGAATCATAATAATTGTAT+AGG | - | chr3.1:3959129-3959148 | None:intergenic | 20.0% |
| ! | AAGAAAAAAGCAAGTGAAAA+TGG | + | chr3.1:3958884-3958903 | MS.gene84349:CDS | 25.0% |
| ! | AAGAATGAATGGAAACAAAT+TGG | + | chr3.1:3958842-3958861 | MS.gene84349:CDS | 25.0% |
| ! | AATTTCACTTTCAAAGATTG+AGG | - | chr3.1:3958596-3958615 | None:intergenic | 25.0% |
| ! | AGAATGAATGGAAACAAATT+GGG | + | chr3.1:3958843-3958862 | MS.gene84349:CDS | 25.0% |
| !! | GTGTTATTCTTAAGAATGAA+TGG | + | chr3.1:3958831-3958850 | MS.gene84349:CDS | 25.0% |
| ACAAATATCACACTCAAACA+CGG | - | chr3.1:3958915-3958934 | None:intergenic | 30.0% | |
| CTTCTTGGATTAACAATTGA+TGG | - | chr3.1:3958557-3958576 | None:intergenic | 30.0% | |
| GAATGAATGGAAACAAATTG+GGG | + | chr3.1:3958844-3958863 | MS.gene84349:CDS | 30.0% | |
| TTCAAAGATTGAGGATTGTT+TGG | - | chr3.1:3958587-3958606 | None:intergenic | 30.0% | |
| ! | ATTTTCCAATGGAAAAGCTT+TGG | + | chr3.1:3958406-3958425 | MS.gene84349:CDS | 30.0% |
| ! | CAATCTAGCAAAGATCTTTT+CGG | + | chr3.1:3958947-3958966 | MS.gene84349:CDS | 30.0% |
| ! | TTTTCCAATGGAAAAGCTTT+GGG | + | chr3.1:3958407-3958426 | MS.gene84349:CDS | 30.0% |
| !! | GTTTTGATCTTTTTCCAGTT+GGG | - | chr3.1:3958676-3958695 | None:intergenic | 30.0% |
| !!! | CTAGATTGTTAGTTTTCCTA+TGG | - | chr3.1:3958985-3959004 | None:intergenic | 30.0% |
| AAAGATTGAGGATTGTTTGG+TGG | - | chr3.1:3958584-3958603 | None:intergenic | 35.0% | |
| AAGATTGAGGATTGTTTGGT+GGG | - | chr3.1:3958583-3958602 | None:intergenic | 35.0% | |
| AGGAAAACTAACAATCTAGC+AGG | + | chr3.1:3958986-3959005 | MS.gene84349:CDS | 35.0% | |
| CAAACCAAGACAGTTTCATA+TGG | + | chr3.1:3958697-3958716 | MS.gene84349:CDS | 35.0% | |
| CTATTCCTACCAAAGTTTGA+CGG | - | chr3.1:3958629-3958648 | None:intergenic | 35.0% | |
| TATTCCTACCAAAGTTTGAC+GGG | - | chr3.1:3958628-3958647 | None:intergenic | 35.0% | |
| TGCTTTGACATCTTTGAATC+TGG | + | chr3.1:3959073-3959092 | MS.gene84349:CDS | 35.0% | |
| !! | CGTTTTGATCTTTTTCCAGT+TGG | - | chr3.1:3958677-3958696 | None:intergenic | 35.0% |
| !! | TGTAACAGGTCATTTTCCAA+TGG | + | chr3.1:3958395-3958414 | MS.gene84349:CDS | 35.0% |
| !!! | TTTTGGATGGATTGGTAGTT+TGG | - | chr3.1:3958456-3958475 | None:intergenic | 35.0% |
| AAATGTACCCCGTCAAACTT+TGG | + | chr3.1:3958617-3958636 | MS.gene84349:CDS | 40.0% | |
| AACAATCTAGCAGGAGAGAT+TGG | + | chr3.1:3958995-3959014 | MS.gene84349:CDS | 40.0% | |
| ACAATCAGCGTATAGCGATT+TGG | + | chr3.1:3958784-3958803 | MS.gene84349:CDS | 40.0% | |
| ATAGAAGCTGCTGAAACACT+AGG | + | chr3.1:3958809-3958828 | MS.gene84349:CDS | 40.0% | |
| ATTCCTACCAAAGTTTGACG+GGG | - | chr3.1:3958627-3958646 | None:intergenic | 40.0% | |
| CTTTGAATCTGGTCACGTAT+TGG | + | chr3.1:3959084-3959103 | MS.gene84349:CDS | 40.0% | |
| GAGTGTGTCTCATTTGTAAC+AGG | + | chr3.1:3958381-3958400 | MS.gene84349:CDS | 40.0% | |
| GATGTAGAGATAGAGTGAGT+TGG | - | chr3.1:3958530-3958549 | None:intergenic | 40.0% | |
| TCAGCCATATGAAACTGTCT+TGG | - | chr3.1:3958704-3958723 | None:intergenic | 40.0% | |
| TCAGGTACTTGGTTGTTGAT+TGG | - | chr3.1:3958482-3958501 | None:intergenic | 40.0% | |
| TCGGACATGAAGATATCCAT+AGG | + | chr3.1:3958966-3958985 | MS.gene84349:CDS | 40.0% | |
| TCTTATCCAACAAACCCAAC+TGG | + | chr3.1:3958659-3958678 | MS.gene84349:CDS | 40.0% | |
| ! | AGGTACTTGGTTGTTGATTG+GGG | - | chr3.1:3958480-3958499 | None:intergenic | 40.0% |
| ! | CAGGTACTTGGTTGTTGATT+GGG | - | chr3.1:3958481-3958500 | None:intergenic | 40.0% |
| ! | CTTTTTCCAGTTGGGTTTGT+TGG | - | chr3.1:3958668-3958687 | None:intergenic | 40.0% |
| ! | GATAGAGTGAGTTGGATGTT+TGG | - | chr3.1:3958522-3958541 | None:intergenic | 40.0% |
| ! | GATTGGTAGTTTGGCATAGT+GGG | - | chr3.1:3958447-3958466 | None:intergenic | 40.0% |
| !! | TTGTTTGGTGGGTTACTTCT+TGG | - | chr3.1:3958572-3958591 | None:intergenic | 40.0% |
| ACCTGAATACTCAGCTGAGT+TGG | + | chr3.1:3958496-3958515 | MS.gene84349:CDS | 45.0% | |
| CAGCTGAGTATTCAGGTACT+TGG | - | chr3.1:3958493-3958512 | None:intergenic | 45.0% | |
| CGTATTGGAGGAACACATGA+AGG | + | chr3.1:3959099-3959118 | MS.gene84349:CDS | 45.0% | |
| TGAATCTGGTCACGTATTGG+AGG | + | chr3.1:3959087-3959106 | MS.gene84349:CDS | 45.0% | |
| ! | GGATTGGTAGTTTGGCATAG+TGG | - | chr3.1:3958448-3958467 | None:intergenic | 45.0% |
| ! | GGTACTTGGTTGTTGATTGG+GGG | - | chr3.1:3958479-3958498 | None:intergenic | 45.0% |
| ! | TCCTCCCAAAGCTTTTCCAT+TGG | - | chr3.1:3958414-3958433 | None:intergenic | 45.0% |
| !! | TCCAATGGAAAAGCTTTGGG+AGG | + | chr3.1:3958410-3958429 | MS.gene84349:CDS | 45.0% |
| !! | TGGTTGTTGATTGGGGGTTT+TGG | - | chr3.1:3958473-3958492 | None:intergenic | 45.0% |
| !!! | ATTGGGGGTTTTGGATGGAT+TGG | - | chr3.1:3958464-3958483 | None:intergenic | 45.0% |
| !!! | TGTTGATTGGGGGTTTTGGA+TGG | - | chr3.1:3958469-3958488 | None:intergenic | 45.0% |
| GCAGGAGAGATTGGTAGAAG+TGG | + | chr3.1:3959004-3959023 | MS.gene84349:CDS | 50.0% | |
| GCCAACTCAGCTGAGTATTC+AGG | - | chr3.1:3958500-3958519 | None:intergenic | 50.0% | |
| GTACCCCGTCAAACTTTGGT+AGG | + | chr3.1:3958621-3958640 | MS.gene84349:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 3958365 | 3959156 | 3958365 | ID=MS.gene84349 |
| chr3.1 | mRNA | 3958365 | 3959156 | 3958365 | ID=MS.gene84349.t1;Parent=MS.gene84349 |
| chr3.1 | exon | 3958365 | 3959156 | 3958365 | ID=MS.gene84349.t1.exon1;Parent=MS.gene84349.t1 |
| chr3.1 | CDS | 3958365 | 3959156 | 3958365 | ID=cds.MS.gene84349.t1;Parent=MS.gene84349.t1 |
| Gene Sequence |
| Protein sequence |
