Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84351.t1 | XP_013459208.1 | 89.1 | 275 | 26 | 1 | 1 | 271 | 1 | 275 | 7.30E-119 | 436.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84351.t1 | Q9M202 | 25.2 | 278 | 150 | 9 | 7 | 262 | 5 | 246 | 2.0e-08 | 61.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84351.t1 | A0A072UTD1 | 89.1 | 275 | 26 | 1 | 1 | 271 | 1 | 275 | 5.3e-119 | 436.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene84351.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84351.t1 | MTR_3g408420 | 90.545 | 275 | 22 | 1 | 1 | 271 | 1 | 275 | 7.58e-179 | 493 |
| MS.gene84351.t1 | MTR_3g008310 | 62.182 | 275 | 100 | 2 | 1 | 271 | 1 | 275 | 1.43e-113 | 328 |
| MS.gene84351.t1 | MTR_3g408380 | 60.791 | 278 | 93 | 4 | 1 | 271 | 1 | 269 | 3.69e-111 | 322 |
| MS.gene84351.t1 | MTR_0160s0030 | 25.231 | 325 | 173 | 11 | 4 | 269 | 2 | 315 | 4.44e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84351.t1 | AT1G02030 | 26.374 | 273 | 149 | 9 | 3 | 264 | 2 | 233 | 1.56e-12 | 66.2 |
| MS.gene84351.t1 | AT3G60580 | 27.698 | 278 | 143 | 11 | 7 | 262 | 5 | 246 | 7.40e-12 | 64.7 |
Find 26 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGTCGTAGACAATTTAATT+TGG | 0.272723 | 3.1:+3984601 | MS.gene84351:CDS |
| AACAATCTATCTGAAGAAAT+TGG | 0.300908 | 3.1:+3984926 | MS.gene84351:CDS |
| TGAGAATACACAAGTTAAAT+TGG | 0.302912 | 3.1:+3984700 | MS.gene84351:CDS |
| ATACTATAGTGAGAAGAAAA+AGG | 0.312924 | 3.1:+3984802 | MS.gene84351:CDS |
| TAAATTGGTGTATAGTGATT+TGG | 0.323391 | 3.1:+3984715 | MS.gene84351:CDS |
| TCAGGTGCTTGGTTGTTGAT+TGG | 0.373001 | 3.1:-3984386 | None:intergenic |
| TATCATACCCTTGTAAACTT+TGG | 0.403944 | 3.1:+3984521 | MS.gene84351:CDS |
| CAACTGAGTGTTCAGGTGCT+TGG | 0.424891 | 3.1:-3984397 | None:intergenic |
| TCTGAAGAAATTGGTATAAG+TGG | 0.426032 | 3.1:+3984935 | MS.gene84351:CDS |
| CTATTATTTGTGTGAATGAA+TGG | 0.435873 | 3.1:+3984762 | MS.gene84351:CDS |
| GTTGACTCAACTGAGTGTTC+AGG | 0.442669 | 3.1:-3984404 | None:intergenic |
| AATAGAGAGAGTTTGATGTT+TGG | 0.448411 | 3.1:-3984426 | None:intergenic |
| TATTCTTACCAAAGTTTACA+AGG | 0.477139 | 3.1:-3984529 | None:intergenic |
| TCACAAGTGCACATATTGCT+TGG | 0.480968 | 3.1:+3984988 | MS.gene84351:CDS |
| CAATGCTTTCCCATTAGAAA+AGG | 0.484168 | 3.1:-3984312 | None:intergenic |
| TGTAGCTGAAGAAAATGAAA+AGG | 0.498469 | 3.1:+3984646 | MS.gene84351:CDS |
| TACAGAAGCTGCTGAAACAA+TGG | 0.521106 | 3.1:+3984739 | MS.gene84351:CDS |
| TGTACTTGAGGAACACAAAA+CGG | 0.523616 | 3.1:+3985030 | MS.gene84351:CDS |
| TGAATCTGATCATGTACTTG+AGG | 0.537940 | 3.1:+3985018 | MS.gene84351:CDS |
| AAGATCGAAGACTATGCATA+GGG | 0.564723 | 3.1:-3984484 | None:intergenic |
| AGAAATTGGTATAAGTGGAA+AGG | 0.574832 | 3.1:+3984940 | MS.gene84351:CDS |
| ATAGTCTTCGATCTTTGAAG+AGG | 0.579889 | 3.1:+3984492 | MS.gene84351:CDS |
| AAAGATCGAAGACTATGCAT+AGG | 0.588065 | 3.1:-3984485 | None:intergenic |
| ATTCTTACCAAAGTTTACAA+GGG | 0.625545 | 3.1:-3984528 | None:intergenic |
| TCTAATGGGAAAGCATTGGG+AGG | 0.669450 | 3.1:+3984317 | MS.gene84351:CDS |
| GAATGAATGGAAACAAGTTG+AGG | 0.698163 | 3.1:+3984775 | MS.gene84351:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAGATTGTTATTTTTCTTA+TGG | - | chr3.1:3984916-3984935 | None:intergenic | 15.0% |
| ! | AACAATCTATCTGAAGAAAT+TGG | + | chr3.1:3984926-3984945 | MS.gene84351:CDS | 25.0% |
| ! | AAGAAAAAGGTTAGTGAAAA+TGG | + | chr3.1:3984815-3984834 | MS.gene84351:CDS | 25.0% |
| ! | ATACTATAGTGAGAAGAAAA+AGG | + | chr3.1:3984802-3984821 | MS.gene84351:CDS | 25.0% |
| ! | ATTCTTACCAAAGTTTACAA+GGG | - | chr3.1:3984531-3984550 | None:intergenic | 25.0% |
| ! | CTATTATTTGTGTGAATGAA+TGG | + | chr3.1:3984762-3984781 | MS.gene84351:CDS | 25.0% |
| ! | TAAATTGGTGTATAGTGATT+TGG | + | chr3.1:3984715-3984734 | MS.gene84351:CDS | 25.0% |
| ! | TATTCTTACCAAAGTTTACA+AGG | - | chr3.1:3984532-3984551 | None:intergenic | 25.0% |
| ! | TGAGAATACACAAGTTAAAT+TGG | + | chr3.1:3984700-3984719 | MS.gene84351:CDS | 25.0% |
| AATAGAGAGAGTTTGATGTT+TGG | - | chr3.1:3984429-3984448 | None:intergenic | 30.0% | |
| AGAAATTGGTATAAGTGGAA+AGG | + | chr3.1:3984940-3984959 | MS.gene84351:CDS | 30.0% | |
| TATCATACCCTTGTAAACTT+TGG | + | chr3.1:3984521-3984540 | MS.gene84351:CDS | 30.0% | |
| TCTGAAGAAATTGGTATAAG+TGG | + | chr3.1:3984935-3984954 | MS.gene84351:CDS | 30.0% | |
| TGTAGCTGAAGAAAATGAAA+AGG | + | chr3.1:3984646-3984665 | MS.gene84351:CDS | 30.0% | |
| ! | GTAACAAGTCCTTTTCTAAT+GGG | + | chr3.1:3984303-3984322 | MS.gene84351:CDS | 30.0% |
| ! | TGGGTTAGAATCAGAAAAAT+TGG | - | chr3.1:3985065-3985084 | None:intergenic | 30.0% |
| ! | TGTAACAAGTCCTTTTCTAA+TGG | + | chr3.1:3984302-3984321 | MS.gene84351:CDS | 30.0% |
| !! | TGTTGATTGGAGATTTTAGA+GGG | - | chr3.1:3984376-3984395 | None:intergenic | 30.0% |
| !! | TTGTTGATTGGAGATTTTAG+AGG | - | chr3.1:3984377-3984396 | None:intergenic | 30.0% |
| !! | TTTTGGATTGGAAATTGATG+AGG | - | chr3.1:3984462-3984481 | None:intergenic | 30.0% |
| !!! | TTTTCTAATGGGAAAGCATT+GGG | + | chr3.1:3984314-3984333 | MS.gene84351:CDS | 30.0% |
| AAAGATCGAAGACTATGCAT+AGG | - | chr3.1:3984488-3984507 | None:intergenic | 35.0% | |
| AAGATCGAAGACTATGCATA+GGG | - | chr3.1:3984487-3984506 | None:intergenic | 35.0% | |
| CAATGCTTTCCCATTAGAAA+AGG | - | chr3.1:3984315-3984334 | None:intergenic | 35.0% | |
| CGGTCGTAGACAATTTAATT+TGG | + | chr3.1:3984601-3984620 | MS.gene84351:CDS | 35.0% | |
| GAATGAATGGAAACAAGTTG+AGG | + | chr3.1:3984775-3984794 | MS.gene84351:CDS | 35.0% | |
| TGAATCTGATCATGTACTTG+AGG | + | chr3.1:3985018-3985037 | MS.gene84351:CDS | 35.0% | |
| TGTACTTGAGGAACACAAAA+CGG | + | chr3.1:3985030-3985049 | MS.gene84351:CDS | 35.0% | |
| ! | ATAGTCTTCGATCTTTGAAG+AGG | + | chr3.1:3984492-3984511 | MS.gene84351:CDS | 35.0% |
| ! | CTTTTTCCAGTTGGGTTTTT+TGG | - | chr3.1:3984572-3984591 | None:intergenic | 35.0% |
| ! | GTTTCGATCTTTTTCCAGTT+GGG | - | chr3.1:3984580-3984599 | None:intergenic | 35.0% |
| ! | TAGCTAAGTGAGTTTTCATG+TGG | - | chr3.1:3984343-3984362 | None:intergenic | 35.0% |
| ! | TCTTTTCCAAAAAACCCAAC+TGG | + | chr3.1:3984563-3984582 | MS.gene84351:CDS | 35.0% |
| !! | ATTGGAGATTTTAGAGGGAT+TGG | - | chr3.1:3984371-3984390 | None:intergenic | 35.0% |
| !! | CTTTTCTAATGGGAAAGCAT+TGG | + | chr3.1:3984313-3984332 | MS.gene84351:CDS | 35.0% |
| !!! | AGACTATGCATAGGGTTTTT+TGG | - | chr3.1:3984479-3984498 | None:intergenic | 35.0% |
| !!! | ATGCATAGGGTTTTTTGGAT+TGG | - | chr3.1:3984474-3984493 | None:intergenic | 35.0% |
| ACTGGAAAAAGATCGAAACG+CGG | + | chr3.1:3984581-3984600 | MS.gene84351:CDS | 40.0% | |
| TACAGAAGCTGCTGAAACAA+TGG | + | chr3.1:3984739-3984758 | MS.gene84351:CDS | 40.0% | |
| TCACAAGTGCACATATTGCT+TGG | + | chr3.1:3984988-3985007 | MS.gene84351:CDS | 40.0% | |
| ! | CGTTTCGATCTTTTTCCAGT+TGG | - | chr3.1:3984581-3984600 | None:intergenic | 40.0% |
| TCAGGTGCTTGGTTGTTGAT+TGG | - | chr3.1:3984389-3984408 | None:intergenic | 45.0% | |
| ! | GTTGACTCAACTGAGTGTTC+AGG | - | chr3.1:3984407-3984426 | None:intergenic | 45.0% |
| !! | TCTAATGGGAAAGCATTGGG+AGG | + | chr3.1:3984317-3984336 | MS.gene84351:CDS | 45.0% |
| CAACTGAGTGTTCAGGTGCT+TGG | - | chr3.1:3984400-3984419 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 3984272 | 3985087 | 3984272 | ID=MS.gene84351 |
| chr3.1 | mRNA | 3984272 | 3985087 | 3984272 | ID=MS.gene84351.t1;Parent=MS.gene84351 |
| chr3.1 | exon | 3984272 | 3985087 | 3984272 | ID=MS.gene84351.t1.exon1;Parent=MS.gene84351.t1 |
| chr3.1 | CDS | 3984272 | 3985087 | 3984272 | ID=cds.MS.gene84351.t1;Parent=MS.gene84351.t1 |
| Gene Sequence |
| Protein sequence |
