AlfalfaGEDB | MS.gene84418

M. sativa cultivar XinJiangDaYe / MS.gene84418

KEGG: K14492
GO: GO:0000160 GO:0007165 GO:0008150 GO:0009987 GO:0035556 GO:0050789 GO:0050794 GO:0065007
PFAM: PF00072.27

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84418.t1	XP_004511028.2	85.6	160	15	1	1	152	45	204	2.80E-67	264.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84418.t1	O80366	67.7	155	40	1	1	145	1	155	1.3e-52	207.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84418.t1	A0A1S2YWH2	85.6	160	15	1	1	152	45	204	2.0e-67	264.6

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene84418.t1	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84418.t1	MTR_8g038620	85.806	155	14	1	1	147	1	155	9.26e-94	269
MS.gene84418.t1	MTR_6g007460	68.780	205	11	2	1	152	3	207	4.44e-93	268
MS.gene84418.t1	MTR_7g490310	69.427	157	39	1	1	148	1	157	8.57e-79	233
MS.gene84418.t1	MTR_3g015490	70.968	155	33	2	7	152	2	153	1.67e-73	217
MS.gene84418.t1	MTR_3g088630	54.717	159	53	3	9	149	22	179	1.22e-55	174
MS.gene84418.t1	MTR_4g106590	57.639	144	60	1	4	146	21	164	1.04e-51	164
MS.gene84418.t1	MTR_3g093860	60.305	131	48	2	10	137	19	148	2.38e-51	161
MS.gene84418.t1	MTR_5g036480	55.479	146	59	2	8	148	15	159	4.36e-51	163
MS.gene84418.t1	MTR_3g078613	51.678	149	72	0	4	152	18	166	5.13e-51	161
MS.gene84418.t1	MTR_1g049100	59.559	136	53	2	8	141	7	142	1.41e-49	158

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84418.t1	AT3G57040	67.742	155	40	1	1	145	1	155	1.18e-73	220
MS.gene84418.t1	AT3G57040	67.742	155	40	1	1	145	1	155	1.18e-73	220
MS.gene84418.t1	AT2G41310	63.871	155	48	1	1	147	1	155	9.67e-71	213
MS.gene84418.t1	AT1G59940	61.832	131	50	0	8	138	32	162	1.40e-55	174
MS.gene84418.t1	AT1G59940	61.832	131	50	0	8	138	124	254	4.91e-54	173
MS.gene84418.t1	AT3G48100	58.273	139	57	1	2	139	18	156	6.10e-54	168
MS.gene84418.t1	AT1G10470	60.305	131	52	0	8	138	19	149	7.33e-54	170
MS.gene84418.t1	AT1G10470	60.305	131	52	0	8	138	33	163	9.20e-54	171
MS.gene84418.t1	AT5G62920	61.538	130	50	0	9	138	25	154	2.09e-52	164
MS.gene84418.t1	AT1G19050	55.147	136	61	0	8	143	23	158	5.42e-52	164

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Find 25 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTTCTTGGTTTGGTTG+AGG	0.449963	4.1:-55993085	MS.gene84418:CDS
ACTCTTGATTTCATCAAATG+AGG	0.455768	4.1:+55992225	None:intergenic
AGAGACTCAGTTCCATGTTT+TGG	0.251951	4.1:-55993381	MS.gene84418:CDS
AGATCATAGCCAGTCATCCC+TGG	0.546401	4.1:+55992904	None:intergenic
AGTAAGGCTTTAAAATTTCT+TGG	0.266953	4.1:-55993096	MS.gene84418:CDS
ATAACAGATTATTGTATGCC+AGG	0.463952	4.1:-55992922	MS.gene84418:CDS
ATGTGGCTTCAGAAGTTCAT+GGG	0.534908	4.1:-55993062	MS.gene84418:CDS
ATGTTCCATCAAGGATTAGC+AGG	0.504757	4.1:-55992458	MS.gene84418:intron
CGGTATGTCTTTAAAAGATT+TGG	0.160260	4.1:+55992503	None:intergenic
CTGATTGTTGAACTGGTTTC+AGG	0.173333	4.1:+55992265	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTCTTGATTTCATCAAATG+AGG	+	chr4.1:55993361-55993380	None:intergenic	30.0%
ATAACAGATTATTGTATGCC+AGG	-	chr4.1:55992661-55992680	MS.gene84418:intron	30.0%
ATGTGAAGTCTCACATATTA+TGG	-	chr4.1:55993241-55993260	MS.gene84418:intron	30.0%
TAACAGATTATTGTATGCCA+GGG	-	chr4.1:55992662-55992681	MS.gene84418:intron	30.0%
TCTGAAGACATAATCACAAT+CGG	+	chr4.1:55993103-55993122	None:intergenic	30.0%
TTTACATCTGATTGTTGAAC+TGG	+	chr4.1:55993328-55993347	None:intergenic	30.0%
TATCATCAACAGCCAAAACA+TGG	+	chr4.1:55992217-55992236	None:intergenic	35.0%
TGATCTGCTCAGAAAAATCA+AGG	-	chr4.1:55992696-55992715	MS.gene84418:intron	35.0%
ATGTGGCTTCAGAAGTTCAT+GGG	-	chr4.1:55992521-55992540	MS.gene84418:intron	40.0%
CTGCTCAGAAAAATCAAGGT+AGG	-	chr4.1:55992700-55992719	MS.gene84418:intron	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	55992188	55993417	55992188	ID=MS.gene84418
chr4.1	mRNA	55992188	55993417	55992188	ID=MS.gene84418.t1;Parent=MS.gene84418
chr4.1	exon	55993315	55993417	55993315	ID=MS.gene84418.t1.exon1;Parent=MS.gene84418.t1
chr4.1	CDS	55993315	55993417	55993315	ID=cds.MS.gene84418.t1;Parent=MS.gene84418.t1
chr4.1	exon	55993062	55993138	55993062	ID=MS.gene84418.t1.exon2;Parent=MS.gene84418.t1
chr4.1	CDS	55993062	55993138	55993062	ID=cds.MS.gene84418.t1;Parent=MS.gene84418.t1
chr4.1	exon	55992888	55992965	55992888	ID=MS.gene84418.t1.exon3;Parent=MS.gene84418.t1
chr4.1	CDS	55992888	55992965	55992888	ID=cds.MS.gene84418.t1;Parent=MS.gene84418.t1
chr4.1	exon	55992459	55992529	55992459	ID=MS.gene84418.t1.exon4;Parent=MS.gene84418.t1
chr4.1	CDS	55992459	55992529	55992459	ID=cds.MS.gene84418.t1;Parent=MS.gene84418.t1
chr4.1	exon	55992188	55992317	55992188	ID=MS.gene84418.t1.exon5;Parent=MS.gene84418.t1
chr4.1	CDS	55992188	55992317	55992188	ID=cds.MS.gene84418.t1;Parent=MS.gene84418.t1

Gene Sequence

>MS.gene84418

ATGGGTATGGCTGCAGAGACTCAGTTCCATGTTTTGGCTGTTGATGATAGTATTATTGATAGAATGTTGATTGAGAGGCTCCTCAAAACTTCTTCTTTTCATGGTACTTTTCTTGTTTATTTTTTTAATTTTGATTTATATTGTAATGATGTAATGTGATGGGAAAAAATATATGAACATAGTTTGTATGGTCAATATACTATTTTGTTGAAGGTGTGATTTTTTTGTTACTAAGATTGACAAATGATAATTATTATTTATTTTGTATTGAAATGATAGTTACTACAATGGACTCTGCTAGTAAGGCTTTAAAATTTCTTGGTTTGGTTGAGGATGTGGCTTCAGAAGTTCATGGGGTAAAATTCTCAAACAATTTTTCTTAATATTATGATGGCTTAGACTATTTTGATGTATAAGTTTGTATTAATATTTGATTCATATTATTCATGCAGGATGTAGATGTAAATCTGATCATAACAGATTATTGTATGCCAGGGATGACTGGCTATGATCTGCTCAGAAAAATCAAGGTAGGTTCTTGAGATATTGCAATACTTTTTTAAATTGATTTTGTCACTTTTTATTTGATTTGTCTATTTTGAATTTCATTAACTTTTTCCATAATTTTGTGCTCATTTTCAAAATTTGAATGTATCAAAAAGTTTTGTGAAATTATTTTGTTTTTCTATTTAGGTAAGAATTTGAAAAATAGTATCATATTTCTTAAATAGAAGTGATTTGGATTTTCGAATCAAAATAATAAAAATCACTTCTGAATCTCTGATTAGTGATTACTGATTTGGGATATTCCCAAAATTATAAAGAAAACAAAGCTCAAACCAAAATTGTTAACTTGATGTTAAAGCTAAACAATTCTTTGATTCTCAGGAATCCAAATCTTTTAAAGACATACCGATTGTGATTATGTCTTCAGAGAATGTTCCATCAAGGATTAGCAGGTGAGAAAATTGTATACATCATTTGTTGAAATCAAGTATCAAATGCAGTTGTGAGCACATGATATCTTATAACACATTACTAATTGTTTGTAAAATGTGAAGTCTCACATATTATGGACTAATTGTTTGTAATTGTATCAGATGTTTTGAAGAGGGAGCTGAAGAATTCTTCCTGAAACCAGTTCAACAATCAGATGTAAACAAGCTGAAGCCTCATTTGATGAAATCAAGAGTTAAGGAAGAGCAAGAACAAACAACCCATCAACAATAA

Protein sequence

>MS.gene84418.t1

MGMAAETQFHVLAVDDSIIDRMLIERLLKTSSFHVTTMDSASKALKFLGLVEDVASEVHGDVDVNLIITDYCMPGMTGYDLLRKIKESKSFKDIPIVIMSSENVPSRISRCFEEGAEEFFLKPVQQSDVNKLKPHLMKSRVKEEQEQTTHQQ