Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84418.t1 | XP_004511028.2 | 85.6 | 160 | 15 | 1 | 1 | 152 | 45 | 204 | 2.80E-67 | 264.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84418.t1 | O80366 | 67.7 | 155 | 40 | 1 | 1 | 145 | 1 | 155 | 1.3e-52 | 207.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84418.t1 | A0A1S2YWH2 | 85.6 | 160 | 15 | 1 | 1 | 152 | 45 | 204 | 2.0e-67 | 264.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene84418.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84418.t1 | MTR_8g038620 | 85.806 | 155 | 14 | 1 | 1 | 147 | 1 | 155 | 9.26e-94 | 269 |
| MS.gene84418.t1 | MTR_6g007460 | 68.780 | 205 | 11 | 2 | 1 | 152 | 3 | 207 | 4.44e-93 | 268 |
| MS.gene84418.t1 | MTR_7g490310 | 69.427 | 157 | 39 | 1 | 1 | 148 | 1 | 157 | 8.57e-79 | 233 |
| MS.gene84418.t1 | MTR_3g015490 | 70.968 | 155 | 33 | 2 | 7 | 152 | 2 | 153 | 1.67e-73 | 217 |
| MS.gene84418.t1 | MTR_3g088630 | 54.717 | 159 | 53 | 3 | 9 | 149 | 22 | 179 | 1.22e-55 | 174 |
| MS.gene84418.t1 | MTR_4g106590 | 57.639 | 144 | 60 | 1 | 4 | 146 | 21 | 164 | 1.04e-51 | 164 |
| MS.gene84418.t1 | MTR_3g093860 | 60.305 | 131 | 48 | 2 | 10 | 137 | 19 | 148 | 2.38e-51 | 161 |
| MS.gene84418.t1 | MTR_5g036480 | 55.479 | 146 | 59 | 2 | 8 | 148 | 15 | 159 | 4.36e-51 | 163 |
| MS.gene84418.t1 | MTR_3g078613 | 51.678 | 149 | 72 | 0 | 4 | 152 | 18 | 166 | 5.13e-51 | 161 |
| MS.gene84418.t1 | MTR_1g049100 | 59.559 | 136 | 53 | 2 | 8 | 141 | 7 | 142 | 1.41e-49 | 158 |
| MS.gene84418.t1 | MTR_3g078613 | 51.333 | 150 | 72 | 1 | 4 | 152 | 18 | 167 | 3.75e-49 | 157 |
| MS.gene84418.t1 | MTR_4g051330 | 74.242 | 66 | 17 | 0 | 87 | 152 | 77 | 142 | 6.66e-31 | 108 |
| MS.gene84418.t1 | MTR_4g061360 | 29.545 | 132 | 84 | 2 | 7 | 138 | 87 | 209 | 4.60e-15 | 71.6 |
| MS.gene84418.t1 | MTR_8g024260 | 29.008 | 131 | 84 | 1 | 11 | 141 | 38 | 159 | 4.68e-15 | 71.6 |
| MS.gene84418.t1 | MTR_8g079940 | 30.769 | 143 | 87 | 3 | 4 | 146 | 24 | 154 | 8.82e-15 | 70.9 |
| MS.gene84418.t1 | MTR_1g067110 | 28.125 | 128 | 83 | 2 | 11 | 138 | 87 | 205 | 3.26e-13 | 66.2 |
| MS.gene84418.t1 | MTR_1g067110 | 28.125 | 128 | 83 | 2 | 11 | 138 | 87 | 205 | 3.30e-13 | 66.2 |
| MS.gene84418.t1 | MTR_7g117705 | 31.429 | 140 | 83 | 4 | 11 | 150 | 11 | 137 | 5.37e-13 | 65.5 |
| MS.gene84418.t1 | MTR_1g013180 | 30.000 | 130 | 79 | 2 | 11 | 140 | 162 | 279 | 1.25e-12 | 64.7 |
| MS.gene84418.t1 | MTR_1g013170 | 29.286 | 140 | 85 | 3 | 11 | 150 | 11 | 136 | 1.51e-12 | 64.3 |
| MS.gene84418.t1 | MTR_3g086100 | 28.676 | 136 | 85 | 2 | 11 | 146 | 19 | 142 | 1.55e-12 | 64.3 |
| MS.gene84418.t1 | MTR_1g032570 | 30.216 | 139 | 85 | 3 | 9 | 147 | 15 | 141 | 1.84e-12 | 63.9 |
| MS.gene84418.t1 | MTR_7g118260 | 26.515 | 132 | 88 | 1 | 7 | 138 | 12 | 134 | 1.83e-11 | 61.2 |
| MS.gene84418.t1 | MTR_7g118260 | 26.515 | 132 | 88 | 1 | 7 | 138 | 12 | 134 | 2.04e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84418.t1 | AT3G57040 | 67.742 | 155 | 40 | 1 | 1 | 145 | 1 | 155 | 1.18e-73 | 220 |
| MS.gene84418.t1 | AT3G57040 | 67.742 | 155 | 40 | 1 | 1 | 145 | 1 | 155 | 1.18e-73 | 220 |
| MS.gene84418.t1 | AT2G41310 | 63.871 | 155 | 48 | 1 | 1 | 147 | 1 | 155 | 9.67e-71 | 213 |
| MS.gene84418.t1 | AT1G59940 | 61.832 | 131 | 50 | 0 | 8 | 138 | 32 | 162 | 1.40e-55 | 174 |
| MS.gene84418.t1 | AT1G59940 | 61.832 | 131 | 50 | 0 | 8 | 138 | 124 | 254 | 4.91e-54 | 173 |
| MS.gene84418.t1 | AT3G48100 | 58.273 | 139 | 57 | 1 | 2 | 139 | 18 | 156 | 6.10e-54 | 168 |
| MS.gene84418.t1 | AT1G10470 | 60.305 | 131 | 52 | 0 | 8 | 138 | 19 | 149 | 7.33e-54 | 170 |
| MS.gene84418.t1 | AT1G10470 | 60.305 | 131 | 52 | 0 | 8 | 138 | 33 | 163 | 9.20e-54 | 171 |
| MS.gene84418.t1 | AT5G62920 | 61.538 | 130 | 50 | 0 | 9 | 138 | 25 | 154 | 2.09e-52 | 164 |
| MS.gene84418.t1 | AT1G19050 | 55.147 | 136 | 61 | 0 | 8 | 143 | 23 | 158 | 5.42e-52 | 164 |
| MS.gene84418.t1 | AT1G74890 | 56.934 | 137 | 59 | 0 | 8 | 144 | 17 | 153 | 9.55e-52 | 164 |
| MS.gene84418.t1 | AT3G56380 | 53.623 | 138 | 63 | 1 | 4 | 140 | 15 | 152 | 4.77e-51 | 160 |
| MS.gene84418.t1 | AT3G56380 | 53.237 | 139 | 63 | 2 | 4 | 140 | 15 | 153 | 3.06e-49 | 155 |
| MS.gene84418.t1 | AT2G40670 | 52.518 | 139 | 57 | 2 | 8 | 140 | 28 | 163 | 4.69e-49 | 155 |
| MS.gene84418.t1 | AT2G40670 | 52.143 | 140 | 57 | 3 | 8 | 140 | 28 | 164 | 3.31e-47 | 150 |
| MS.gene84418.t1 | AT2G07440 | 65.574 | 61 | 21 | 0 | 87 | 147 | 9 | 69 | 8.05e-23 | 87.8 |
| MS.gene84418.t1 | AT1G10470 | 58.491 | 53 | 22 | 0 | 86 | 138 | 35 | 87 | 3.29e-16 | 72.0 |
| MS.gene84418.t1 | AT2G25180 | 32.308 | 130 | 76 | 3 | 11 | 140 | 19 | 136 | 8.58e-14 | 67.8 |
| MS.gene84418.t1 | AT5G07210 | 28.472 | 144 | 90 | 3 | 8 | 151 | 15 | 145 | 1.90e-13 | 67.0 |
| MS.gene84418.t1 | AT5G60100 | 30.469 | 128 | 80 | 2 | 11 | 138 | 66 | 184 | 6.39e-13 | 65.5 |
| MS.gene84418.t1 | AT5G60100 | 30.469 | 128 | 80 | 2 | 11 | 138 | 66 | 184 | 6.39e-13 | 65.5 |
| MS.gene84418.t1 | AT5G60100 | 30.469 | 128 | 80 | 2 | 11 | 138 | 66 | 184 | 6.39e-13 | 65.5 |
| MS.gene84418.t1 | AT5G60100 | 30.469 | 128 | 80 | 2 | 11 | 138 | 66 | 184 | 7.89e-13 | 65.1 |
| MS.gene84418.t1 | AT5G02810 | 27.344 | 128 | 84 | 2 | 11 | 138 | 80 | 198 | 9.20e-13 | 65.1 |
| MS.gene84418.t1 | AT1G67710 | 28.148 | 135 | 85 | 2 | 9 | 143 | 11 | 133 | 1.07e-12 | 64.7 |
| MS.gene84418.t1 | AT5G60100 | 30.469 | 128 | 80 | 2 | 11 | 138 | 66 | 184 | 1.46e-12 | 64.3 |
| MS.gene84418.t1 | AT5G60100 | 30.469 | 128 | 80 | 2 | 11 | 138 | 66 | 184 | 1.46e-12 | 64.3 |
| MS.gene84418.t1 | AT5G60100 | 30.469 | 128 | 80 | 2 | 11 | 138 | 66 | 184 | 1.46e-12 | 64.3 |
| MS.gene84418.t1 | AT3G16857 | 28.889 | 135 | 84 | 3 | 9 | 143 | 37 | 159 | 1.00e-11 | 62.0 |
| MS.gene84418.t1 | AT3G16857 | 28.889 | 135 | 84 | 3 | 9 | 143 | 37 | 159 | 1.01e-11 | 62.0 |
| MS.gene84418.t1 | AT2G46790 | 25.170 | 147 | 100 | 3 | 6 | 152 | 34 | 170 | 1.93e-11 | 61.2 |
| MS.gene84418.t1 | AT2G46790 | 25.170 | 147 | 100 | 3 | 6 | 152 | 34 | 170 | 1.96e-11 | 61.2 |
| MS.gene84418.t1 | AT1G49190 | 28.788 | 132 | 82 | 3 | 10 | 141 | 35 | 154 | 3.19e-11 | 60.5 |
| MS.gene84418.t1 | AT5G24470 | 27.820 | 133 | 85 | 2 | 9 | 140 | 50 | 172 | 5.27e-11 | 60.1 |
Find 25 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGTATGTCTTTAAAAGATT+TGG | 0.160260 | 4.1:+55992503 | None:intergenic |
| CTGATTGTTGAACTGGTTTC+AGG | 0.173333 | 4.1:+55992265 | None:intergenic |
| AGAGACTCAGTTCCATGTTT+TGG | 0.251951 | 4.1:-55993381 | MS.gene84418:CDS |
| AGTAAGGCTTTAAAATTTCT+TGG | 0.266953 | 4.1:-55993096 | MS.gene84418:CDS |
| TCTCACCTGCTAATCCTTGA+TGG | 0.304860 | 4.1:+55992453 | None:intergenic |
| GGCTTTAAAATTTCTTGGTT+TGG | 0.308229 | 4.1:-55993091 | MS.gene84418:CDS |
| TTTACATCTGATTGTTGAAC+TGG | 0.343425 | 4.1:+55992258 | None:intergenic |
| TCTTGGTTTGGTTGAGGATG+TGG | 0.414547 | 4.1:-55993079 | MS.gene84418:CDS |
| TTTGATGAAATCAAGAGTTA+AGG | 0.416978 | 4.1:-55992220 | MS.gene84418:CDS |
| TACAATGGACTCTGCTAGTA+AGG | 0.427203 | 4.1:-55993112 | MS.gene84418:CDS |
| TATTGTATGCCAGGGATGAC+TGG | 0.449232 | 4.1:-55992913 | MS.gene84418:CDS |
| AAAATTTCTTGGTTTGGTTG+AGG | 0.449963 | 4.1:-55993085 | MS.gene84418:CDS |
| ACTCTTGATTTCATCAAATG+AGG | 0.455768 | 4.1:+55992225 | None:intergenic |
| ATAACAGATTATTGTATGCC+AGG | 0.463952 | 4.1:-55992922 | MS.gene84418:CDS |
| ATGTTCCATCAAGGATTAGC+AGG | 0.504757 | 4.1:-55992458 | MS.gene84418:intron |
| TTGATAGAATGTTGATTGAG+AGG | 0.516155 | 4.1:-55993340 | MS.gene84418:CDS |
| GATGTGGCTTCAGAAGTTCA+TGG | 0.523538 | 4.1:-55993063 | MS.gene84418:CDS |
| ATGTGGCTTCAGAAGTTCAT+GGG | 0.534908 | 4.1:-55993062 | MS.gene84418:CDS |
| CTTCAGAGAATGTTCCATCA+AGG | 0.539392 | 4.1:-55992467 | MS.gene84418:CDS |
| AGATCATAGCCAGTCATCCC+TGG | 0.546401 | 4.1:+55992904 | None:intergenic |
| TCTGAAGACATAATCACAAT+CGG | 0.548679 | 4.1:+55992483 | None:intergenic |
| TAACAGATTATTGTATGCCA+GGG | 0.564090 | 4.1:-55992921 | MS.gene84418:CDS |
| TATCATCAACAGCCAAAACA+TGG | 0.580138 | 4.1:+55993369 | None:intergenic |
| TGAAATGATAGTTACTACAA+TGG | 0.624009 | 4.1:-55993127 | MS.gene84418:intron |
| TGTGGCTTCAGAAGTTCATG+GGG | 0.695893 | 4.1:-55993061 | MS.gene84418:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTTCTTAATATTATGA+TGG | - | chr4.1:55992559-55992578 | MS.gene84418:intron | 10.0% |
| !!! | ATTATTTTGTTTTTCTATTT+AGG | - | chr4.1:55992859-55992878 | MS.gene84418:intron | 10.0% |
| !!! | TTTGTTTTCTTTATAATTTT+GGG | + | chr4.1:55993000-55993019 | None:intergenic | 10.0% |
| !!! | CTTTGTTTTCTTTATAATTT+TGG | + | chr4.1:55993001-55993020 | None:intergenic | 15.0% |
| !! | TTTCTTAAATAGAAGTGATT+TGG | - | chr4.1:55992907-55992926 | MS.gene84418:CDS | 20.0% |
| !!! | ATATATGAACATAGTTTGTA+TGG | - | chr4.1:55992356-55992375 | MS.gene84418:intron | 20.0% |
| !!! | TAACATCAAGTTAACAATTT+TGG | + | chr4.1:55993030-55993049 | None:intergenic | 20.0% |
| !!! | TCAATATACTATTTTGTTGA+AGG | - | chr4.1:55992379-55992398 | MS.gene84418:intron | 20.0% |
| ! | ATTGTAATGATGTAATGTGA+TGG | - | chr4.1:55992327-55992346 | MS.gene84418:intron | 25.0% |
| ! | TAAACAATTCTTTGATTCTC+AGG | - | chr4.1:55993054-55993073 | MS.gene84418:intron | 25.0% |
| ! | TGAAAATGAGCACAAAATTA+TGG | + | chr4.1:55992809-55992828 | None:intergenic | 25.0% |
| ! | TGAAATGATAGTTACTACAA+TGG | - | chr4.1:55992456-55992475 | MS.gene84418:intron | 25.0% |
| ! | TTGATTCATATTATTCATGC+AGG | - | chr4.1:55992618-55992637 | MS.gene84418:intron | 25.0% |
| ! | TTGTAATGATGTAATGTGAT+GGG | - | chr4.1:55992328-55992347 | MS.gene84418:intron | 25.0% |
| ! | TTTGATGAAATCAAGAGTTA+AGG | - | chr4.1:55993363-55993382 | MS.gene84418:CDS | 25.0% |
| !!! | AGTAAGGCTTTAAAATTTCT+TGG | - | chr4.1:55992487-55992506 | MS.gene84418:CDS | 25.0% |
| !!! | TGATTAGTGATTACTGATTT+GGG | - | chr4.1:55992969-55992988 | MS.gene84418:intron | 25.0% |
| ACTCTTGATTTCATCAAATG+AGG | + | chr4.1:55993361-55993380 | None:intergenic | 30.0% | |
| ATAACAGATTATTGTATGCC+AGG | - | chr4.1:55992661-55992680 | MS.gene84418:intron | 30.0% | |
| ATGTGAAGTCTCACATATTA+TGG | - | chr4.1:55993241-55993260 | MS.gene84418:intron | 30.0% | |
| TAACAGATTATTGTATGCCA+GGG | - | chr4.1:55992662-55992681 | MS.gene84418:intron | 30.0% | |
| TCTGAAGACATAATCACAAT+CGG | + | chr4.1:55993103-55993122 | None:intergenic | 30.0% | |
| TTTACATCTGATTGTTGAAC+TGG | + | chr4.1:55993328-55993347 | None:intergenic | 30.0% | |
| ! | CATGAAAAGAAGAAGTTTTG+AGG | + | chr4.1:55992271-55992290 | None:intergenic | 30.0% |
| ! | CGGTATGTCTTTAAAAGATT+TGG | + | chr4.1:55993083-55993102 | None:intergenic | 30.0% |
| ! | CTCAAAACTTCTTCTTTTCA+TGG | - | chr4.1:55992269-55992288 | MS.gene84418:CDS | 30.0% |
| ! | CTGATTAGTGATTACTGATT+TGG | - | chr4.1:55992968-55992987 | MS.gene84418:intron | 30.0% |
| ! | TTGATAGAATGTTGATTGAG+AGG | - | chr4.1:55992243-55992262 | MS.gene84418:CDS | 30.0% |
| !! | AAAATTTCTTGGTTTGGTTG+AGG | - | chr4.1:55992498-55992517 | MS.gene84418:CDS | 30.0% |
| !! | GGCTTTAAAATTTCTTGGTT+TGG | - | chr4.1:55992492-55992511 | MS.gene84418:CDS | 30.0% |
| !!! | TGTATCAGATGTTTTGAAGA+GGG | - | chr4.1:55993280-55993299 | MS.gene84418:intron | 30.0% |
| !!! | TTGTATCAGATGTTTTGAAG+AGG | - | chr4.1:55993279-55993298 | MS.gene84418:intron | 30.0% |
| TATCATCAACAGCCAAAACA+TGG | + | chr4.1:55992217-55992236 | None:intergenic | 35.0% | |
| TGATCTGCTCAGAAAAATCA+AGG | - | chr4.1:55992696-55992715 | MS.gene84418:intron | 35.0% | |
| ATGTGGCTTCAGAAGTTCAT+GGG | - | chr4.1:55992521-55992540 | MS.gene84418:intron | 40.0% | |
| CTGCTCAGAAAAATCAAGGT+AGG | - | chr4.1:55992700-55992719 | MS.gene84418:intron | 40.0% | |
| CTTCAGAGAATGTTCCATCA+AGG | - | chr4.1:55993116-55993135 | MS.gene84418:CDS | 40.0% | |
| ! | AGAGACTCAGTTCCATGTTT+TGG | - | chr4.1:55992202-55992221 | MS.gene84418:CDS | 40.0% |
| ! | ATGTTCCATCAAGGATTAGC+AGG | - | chr4.1:55993125-55993144 | MS.gene84418:intron | 40.0% |
| ! | CTGATTGTTGAACTGGTTTC+AGG | + | chr4.1:55993321-55993340 | None:intergenic | 40.0% |
| ! | TACAATGGACTCTGCTAGTA+AGG | - | chr4.1:55992471-55992490 | MS.gene84418:CDS | 40.0% |
| GATGTGGCTTCAGAAGTTCA+TGG | - | chr4.1:55992520-55992539 | MS.gene84418:intron | 45.0% | |
| TATTGTATGCCAGGGATGAC+TGG | - | chr4.1:55992670-55992689 | MS.gene84418:intron | 45.0% | |
| TCTCACCTGCTAATCCTTGA+TGG | + | chr4.1:55993133-55993152 | None:intergenic | 45.0% | |
| TGTGGCTTCAGAAGTTCATG+GGG | - | chr4.1:55992522-55992541 | MS.gene84418:intron | 45.0% | |
| !! | TCTTGGTTTGGTTGAGGATG+TGG | - | chr4.1:55992504-55992523 | MS.gene84418:CDS | 45.0% |
| AGATCATAGCCAGTCATCCC+TGG | + | chr4.1:55992682-55992701 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 55992188 | 55993417 | 55992188 | ID=MS.gene84418 |
| chr4.1 | mRNA | 55992188 | 55993417 | 55992188 | ID=MS.gene84418.t1;Parent=MS.gene84418 |
| chr4.1 | exon | 55993315 | 55993417 | 55993315 | ID=MS.gene84418.t1.exon1;Parent=MS.gene84418.t1 |
| chr4.1 | CDS | 55993315 | 55993417 | 55993315 | ID=cds.MS.gene84418.t1;Parent=MS.gene84418.t1 |
| chr4.1 | exon | 55993062 | 55993138 | 55993062 | ID=MS.gene84418.t1.exon2;Parent=MS.gene84418.t1 |
| chr4.1 | CDS | 55993062 | 55993138 | 55993062 | ID=cds.MS.gene84418.t1;Parent=MS.gene84418.t1 |
| chr4.1 | exon | 55992888 | 55992965 | 55992888 | ID=MS.gene84418.t1.exon3;Parent=MS.gene84418.t1 |
| chr4.1 | CDS | 55992888 | 55992965 | 55992888 | ID=cds.MS.gene84418.t1;Parent=MS.gene84418.t1 |
| chr4.1 | exon | 55992459 | 55992529 | 55992459 | ID=MS.gene84418.t1.exon4;Parent=MS.gene84418.t1 |
| chr4.1 | CDS | 55992459 | 55992529 | 55992459 | ID=cds.MS.gene84418.t1;Parent=MS.gene84418.t1 |
| chr4.1 | exon | 55992188 | 55992317 | 55992188 | ID=MS.gene84418.t1.exon5;Parent=MS.gene84418.t1 |
| chr4.1 | CDS | 55992188 | 55992317 | 55992188 | ID=cds.MS.gene84418.t1;Parent=MS.gene84418.t1 |
| Gene Sequence |
| Protein sequence |
