Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84508.t1 | AES70699.1 | 96.1 | 127 | 5 | 0 | 1 | 127 | 1 | 127 | 9.80E-66 | 259.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84508.t1 | Q851V5 | 46.3 | 67 | 30 | 2 | 58 | 122 | 963 | 1025 | 4.2e-09 | 62.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84508.t1 | G8A2Z9 | 96.1 | 127 | 5 | 0 | 1 | 127 | 1 | 127 | 7.1e-66 | 259.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene84508.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84508.t1 | MTR_3g061300 | 96.063 | 127 | 5 | 0 | 1 | 127 | 1 | 127 | 2.27e-90 | 257 |
| MS.gene84508.t1 | MTR_3g061340 | 76.860 | 121 | 28 | 0 | 2 | 122 | 153 | 273 | 4.11e-64 | 196 |
| MS.gene84508.t1 | MTR_3g061370 | 69.421 | 121 | 37 | 0 | 2 | 122 | 160 | 280 | 3.43e-54 | 171 |
| MS.gene84508.t1 | MTR_3g061350 | 68.595 | 121 | 38 | 0 | 2 | 122 | 163 | 283 | 7.93e-53 | 167 |
| MS.gene84508.t1 | MTR_3g061320 | 71.429 | 112 | 32 | 0 | 11 | 122 | 167 | 278 | 1.88e-50 | 161 |
| MS.gene84508.t1 | MTR_3g061310 | 60.976 | 123 | 46 | 1 | 2 | 122 | 162 | 284 | 1.45e-42 | 141 |
| MS.gene84508.t1 | MTR_3g061330 | 66.667 | 90 | 29 | 1 | 2 | 90 | 162 | 251 | 8.69e-35 | 120 |
| MS.gene84508.t1 | MTR_8g103940 | 37.391 | 115 | 62 | 4 | 14 | 122 | 383 | 493 | 2.68e-16 | 73.9 |
| MS.gene84508.t1 | MTR_1g108780 | 37.168 | 113 | 66 | 3 | 14 | 122 | 345 | 456 | 1.31e-14 | 69.3 |
| MS.gene84508.t1 | MTR_1g108780 | 32.727 | 110 | 60 | 5 | 22 | 126 | 192 | 292 | 1.07e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84508.t1 | AT1G49480 | 32.110 | 109 | 64 | 4 | 17 | 120 | 114 | 217 | 1.13e-11 | 60.1 |
| MS.gene84508.t1 | AT1G49480 | 32.110 | 109 | 64 | 4 | 17 | 120 | 114 | 217 | 1.13e-11 | 60.1 |
| MS.gene84508.t1 | AT1G49480 | 32.110 | 109 | 64 | 4 | 17 | 120 | 114 | 217 | 1.13e-11 | 60.1 |
Find 28 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGTGTGTTCCAACTTTAAA+TGG | 0.136813 | 3.1:+45448186 | MS.gene84508:intron |
| CAGATATTAACTGTCCCTTT+TGG | 0.351295 | 3.1:-45448418 | None:intergenic |
| ACATTTCTAGCCTCACAATA+AGG | 0.400255 | 3.1:-45448279 | None:intergenic |
| TCAATAACCCCATTTAAAGT+TGG | 0.400471 | 3.1:-45448195 | None:intergenic |
| TCCTGAGCTCTAGCTCCCTT+TGG | 0.408256 | 3.1:-45447977 | None:intergenic |
| AAAGAACTACATCATGGAAT+TGG | 0.417108 | 3.1:+45447954 | MS.gene84508:CDS |
| AGTGTGTTCCAACTTTAAAT+GGG | 0.449955 | 3.1:+45448187 | MS.gene84508:intron |
| TTAATGTTCATATTACCAAA+AGG | 0.460659 | 3.1:+45448403 | MS.gene84508:CDS |
| TCTTGCAAACAAGGACCAAC+CGG | 0.476293 | 3.1:-45448316 | None:intergenic |
| TAATGTTCATATTACCAAAA+GGG | 0.486138 | 3.1:+45448404 | MS.gene84508:CDS |
| AATGTTCGCCGCCTCTCGGC+CGG | 0.489655 | 3.1:+45448297 | MS.gene84508:CDS |
| AGTATGTGATGCTGCAGATT+GGG | 0.492313 | 3.1:+45448229 | MS.gene84508:CDS |
| ACCACTTTCTCTTGCAAACA+AGG | 0.504540 | 3.1:-45448325 | None:intergenic |
| TAGAAATGTTCGCCGCCTCT+CGG | 0.526048 | 3.1:+45448293 | MS.gene84508:CDS |
| TCCTTGTTTGCAAGAGAAAG+TGG | 0.529022 | 3.1:+45448324 | MS.gene84508:CDS |
| ACATCATGGAATTGGCCAAA+GGG | 0.541191 | 3.1:+45447962 | MS.gene84508:CDS |
| GCCAAAGGGAGCTAGAGCTC+AGG | 0.549833 | 3.1:+45447976 | MS.gene84508:CDS |
| TTCGCCGCCTCTCGGCCGGT+TGG | 0.551560 | 3.1:+45448301 | MS.gene84508:CDS |
| AGAGAAAGTGGTCTACAAGT+AGG | 0.556643 | 3.1:+45448336 | MS.gene84508:CDS |
| TGCAGATTGGGAAAAGATCA+TGG | 0.571416 | 3.1:+45448241 | MS.gene84508:CDS |
| AAGTATGTGATGCTGCAGAT+TGG | 0.577298 | 3.1:+45448228 | MS.gene84508:CDS |
| CTAGTGAAAGAACTACATCA+TGG | 0.582826 | 3.1:+45447948 | MS.gene84508:CDS |
| AGGACCAACCGGCCGAGAGG+CGG | 0.587468 | 3.1:-45448305 | None:intergenic |
| TACATCATGGAATTGGCCAA+AGG | 0.610272 | 3.1:+45447961 | MS.gene84508:CDS |
| GAAACTGCTTCCTTATTGTG+AGG | 0.617392 | 3.1:+45448269 | MS.gene84508:CDS |
| GTGTGTTCCAACTTTAAATG+GGG | 0.619238 | 3.1:+45448188 | MS.gene84508:intron |
| ACAAGGACCAACCGGCCGAG+AGG | 0.636650 | 3.1:-45448308 | None:intergenic |
| TCATCTTGCAGAAAATCGAG+TGG | 0.664922 | 3.1:+45448057 | MS.gene84508:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGTTCATATTACCAAAA+GGG | + | chr3.1:45448404-45448423 | MS.gene84508:CDS | 20.0% |
| !! | TTAATGTTCATATTACCAAA+AGG | + | chr3.1:45448403-45448422 | MS.gene84508:CDS | 20.0% |
| !! | TTTATGAGAATTGTGAAAAA+AGG | - | chr3.1:45448031-45448050 | None:intergenic | 20.0% |
| AAAGAACTACATCATGGAAT+TGG | + | chr3.1:45447954-45447973 | MS.gene84508:CDS | 30.0% | |
| AGTGTGTTCCAACTTTAAAT+GGG | + | chr3.1:45448187-45448206 | MS.gene84508:intron | 30.0% | |
| ! | TCAATAACCCCATTTAAAGT+TGG | - | chr3.1:45448198-45448217 | None:intergenic | 30.0% |
| CAGTGTGTTCCAACTTTAAA+TGG | + | chr3.1:45448186-45448205 | MS.gene84508:intron | 35.0% | |
| CTAGTGAAAGAACTACATCA+TGG | + | chr3.1:45447948-45447967 | MS.gene84508:CDS | 35.0% | |
| GTGTGTTCCAACTTTAAATG+GGG | + | chr3.1:45448188-45448207 | MS.gene84508:intron | 35.0% | |
| ! | GATTTTCTGCAAGATGAGTA+GGG | - | chr3.1:45448054-45448073 | None:intergenic | 35.0% |
| !! | ACATTTCTAGCCTCACAATA+AGG | - | chr3.1:45448282-45448301 | None:intergenic | 35.0% |
| AAGTATGTGATGCTGCAGAT+TGG | + | chr3.1:45448228-45448247 | MS.gene84508:CDS | 40.0% | |
| ACATCATGGAATTGGCCAAA+GGG | + | chr3.1:45447962-45447981 | MS.gene84508:CDS | 40.0% | |
| ACCACTTTCTCTTGCAAACA+AGG | - | chr3.1:45448328-45448347 | None:intergenic | 40.0% | |
| AGAGAAAGTGGTCTACAAGT+AGG | + | chr3.1:45448336-45448355 | MS.gene84508:CDS | 40.0% | |
| AGTATGTGATGCTGCAGATT+GGG | + | chr3.1:45448229-45448248 | MS.gene84508:CDS | 40.0% | |
| GAAACTGCTTCCTTATTGTG+AGG | + | chr3.1:45448269-45448288 | MS.gene84508:CDS | 40.0% | |
| TACATCATGGAATTGGCCAA+AGG | + | chr3.1:45447961-45447980 | MS.gene84508:CDS | 40.0% | |
| TCATCTTGCAGAAAATCGAG+TGG | + | chr3.1:45448057-45448076 | MS.gene84508:CDS | 40.0% | |
| TGCAGATTGGGAAAAGATCA+TGG | + | chr3.1:45448241-45448260 | MS.gene84508:CDS | 40.0% | |
| TTGCAGAAAATCGAGTGGTA+AGG | + | chr3.1:45448062-45448081 | MS.gene84508:intron | 40.0% | |
| ! | CGATTTTCTGCAAGATGAGT+AGG | - | chr3.1:45448055-45448074 | None:intergenic | 40.0% |
| ! | TCCTTGTTTGCAAGAGAAAG+TGG | + | chr3.1:45448324-45448343 | MS.gene84508:CDS | 40.0% |
| TCTTGCAAACAAGGACCAAC+CGG | - | chr3.1:45448319-45448338 | None:intergenic | 45.0% | |
| TAGAAATGTTCGCCGCCTCT+CGG | + | chr3.1:45448293-45448312 | MS.gene84508:CDS | 50.0% | |
| ! | TCCTGAGCTCTAGCTCCCTT+TGG | - | chr3.1:45447980-45447999 | None:intergenic | 55.0% |
| GCCAAAGGGAGCTAGAGCTC+AGG | + | chr3.1:45447976-45447995 | MS.gene84508:CDS | 60.0% | |
| AATGTTCGCCGCCTCTCGGC+CGG | + | chr3.1:45448297-45448316 | MS.gene84508:CDS | 65.0% | |
| ACAAGGACCAACCGGCCGAG+AGG | - | chr3.1:45448311-45448330 | None:intergenic | 65.0% | |
| AGGACCAACCGGCCGAGAGG+CGG | - | chr3.1:45448308-45448327 | None:intergenic | 70.0% | |
| TTCGCCGCCTCTCGGCCGGT+TGG | + | chr3.1:45448301-45448320 | MS.gene84508:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 45447941 | 45448434 | 45447941 | ID=MS.gene84508 |
| chr3.1 | mRNA | 45447941 | 45448434 | 45447941 | ID=MS.gene84508.t1;Parent=MS.gene84508 |
| chr3.1 | exon | 45447941 | 45448078 | 45447941 | ID=MS.gene84508.t1.exon1;Parent=MS.gene84508.t1 |
| chr3.1 | CDS | 45447941 | 45448078 | 45447941 | ID=cds.MS.gene84508.t1;Parent=MS.gene84508.t1 |
| chr3.1 | exon | 45448189 | 45448434 | 45448189 | ID=MS.gene84508.t1.exon2;Parent=MS.gene84508.t1 |
| chr3.1 | CDS | 45448189 | 45448434 | 45448189 | ID=cds.MS.gene84508.t1;Parent=MS.gene84508.t1 |
| Gene Sequence |
| Protein sequence |
