Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84513.t1 | AES70699.1 | 94.5 | 127 | 7 | 0 | 1 | 127 | 1 | 127 | 6.40E-65 | 256.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84513.t1 | Q851V5 | 46.3 | 67 | 30 | 2 | 58 | 122 | 963 | 1025 | 4.2e-09 | 62.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84513.t1 | G8A2Z9 | 94.5 | 127 | 7 | 0 | 1 | 127 | 1 | 127 | 4.6e-65 | 256.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene84513.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84513.t1 | MTR_3g061300 | 94.488 | 127 | 7 | 0 | 1 | 127 | 1 | 127 | 8.48e-90 | 256 |
| MS.gene84513.t1 | MTR_3g061340 | 76.860 | 121 | 28 | 0 | 2 | 122 | 153 | 273 | 9.51e-64 | 195 |
| MS.gene84513.t1 | MTR_3g061370 | 69.421 | 121 | 37 | 0 | 2 | 122 | 160 | 280 | 4.12e-54 | 171 |
| MS.gene84513.t1 | MTR_3g061350 | 68.595 | 121 | 38 | 0 | 2 | 122 | 163 | 283 | 1.09e-52 | 167 |
| MS.gene84513.t1 | MTR_3g061320 | 71.429 | 112 | 32 | 0 | 11 | 122 | 167 | 278 | 1.41e-50 | 162 |
| MS.gene84513.t1 | MTR_3g061310 | 61.789 | 123 | 45 | 1 | 2 | 122 | 162 | 284 | 7.77e-43 | 142 |
| MS.gene84513.t1 | MTR_3g061330 | 64.444 | 90 | 31 | 1 | 2 | 90 | 162 | 251 | 2.36e-33 | 117 |
| MS.gene84513.t1 | MTR_8g103940 | 38.261 | 115 | 61 | 4 | 14 | 122 | 383 | 493 | 9.75e-17 | 75.5 |
| MS.gene84513.t1 | MTR_1g108780 | 38.053 | 113 | 65 | 3 | 14 | 122 | 345 | 456 | 4.55e-15 | 70.5 |
| MS.gene84513.t1 | MTR_1g108780 | 31.818 | 110 | 61 | 5 | 22 | 126 | 192 | 292 | 3.08e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84513.t1 | AT1G49480 | 32.110 | 109 | 64 | 4 | 17 | 120 | 114 | 217 | 1.35e-11 | 59.7 |
| MS.gene84513.t1 | AT1G49480 | 32.110 | 109 | 64 | 4 | 17 | 120 | 114 | 217 | 1.35e-11 | 59.7 |
| MS.gene84513.t1 | AT1G49480 | 32.110 | 109 | 64 | 4 | 17 | 120 | 114 | 217 | 1.35e-11 | 59.7 |
Find 29 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGTGTGTACCAACTTTAAA+TGG | 0.174261 | 3.1:+45661616 | MS.gene84513:intron |
| TCCTGAGCTTTAGCTCCCTT+TGG | 0.354671 | 3.1:-45661414 | None:intergenic |
| ACTGATAAACAAGAAAGATT+TGG | 0.358474 | 3.1:+45661807 | MS.gene84513:CDS |
| CTACTCACCTAGCAGAAAAT+CGG | 0.374095 | 3.1:+45661490 | MS.gene84513:CDS |
| AAAGAACTACATCATGGAAT+TGG | 0.395138 | 3.1:+45661391 | MS.gene84513:CDS |
| GATACATTAAGTGTCACTTT+TGG | 0.397798 | 3.1:-45661848 | None:intergenic |
| ACATTTCTAGCCTCACAATA+AGG | 0.400255 | 3.1:-45661709 | None:intergenic |
| TCAATAACCCCATTTAAAGT+TGG | 0.446011 | 3.1:-45661625 | None:intergenic |
| AGTGTGTACCAACTTTAAAT+GGG | 0.455268 | 3.1:+45661617 | MS.gene84513:intron |
| ACCACTTTCTCTTGCAAACA+AGG | 0.468664 | 3.1:-45661755 | None:intergenic |
| TCTTGCAAACAAGGACCAAC+CGG | 0.476293 | 3.1:-45661746 | None:intergenic |
| AATGTTCGCCGCCTCTCGGC+CGG | 0.489655 | 3.1:+45661727 | MS.gene84513:CDS |
| AGTATGTGATGCTGCAGATT+GGG | 0.492313 | 3.1:+45661659 | MS.gene84513:CDS |
| TAGAAATGTTCGCCGCCTCT+CGG | 0.526048 | 3.1:+45661723 | MS.gene84513:CDS |
| TCCTTGTTTGCAAGAGAAAG+TGG | 0.529022 | 3.1:+45661754 | MS.gene84513:CDS |
| ACATCATGGAATTGGCCAAA+GGG | 0.541191 | 3.1:+45661399 | MS.gene84513:CDS |
| AGAGAAAGTGGTCTGCAAGT+AGG | 0.546476 | 3.1:+45661766 | MS.gene84513:CDS |
| TACTCACCTAGCAGAAAATC+GGG | 0.546681 | 3.1:+45661491 | MS.gene84513:CDS |
| TTCGCCGCCTCTCGGCCGGT+TGG | 0.551560 | 3.1:+45661731 | MS.gene84513:CDS |
| GCCAAAGGGAGCTAAAGCTC+AGG | 0.559420 | 3.1:+45661413 | MS.gene84513:CDS |
| TGCAGATTGGGAAAAGATCA+TGG | 0.571416 | 3.1:+45661671 | MS.gene84513:CDS |
| CTAATCAAAGAACTACATCA+TGG | 0.573456 | 3.1:+45661385 | MS.gene84513:CDS |
| AAGTATGTGATGCTGCAGAT+TGG | 0.577298 | 3.1:+45661658 | MS.gene84513:CDS |
| AGGACCAACCGGCCGAGAGG+CGG | 0.587468 | 3.1:-45661735 | None:intergenic |
| TACATCATGGAATTGGCCAA+AGG | 0.610272 | 3.1:+45661398 | MS.gene84513:CDS |
| GAAACTGCTTCCTTATTGTG+AGG | 0.617392 | 3.1:+45661699 | MS.gene84513:CDS |
| ACAAGGACCAACCGGCCGAG+AGG | 0.636650 | 3.1:-45661738 | None:intergenic |
| GTGTGTACCAACTTTAAATG+GGG | 0.652978 | 3.1:+45661618 | MS.gene84513:intron |
| TCACCTAGCAGAAAATCGGG+TGG | 0.717171 | 3.1:+45661494 | MS.gene84513:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTATGAGAATTGTGAAAAA+AGG | - | chr3.1:45661468-45661487 | None:intergenic | 20.0% |
| ! | ACTGATAAACAAGAAAGATT+TGG | + | chr3.1:45661807-45661826 | MS.gene84513:CDS | 25.0% |
| AAAGAACTACATCATGGAAT+TGG | + | chr3.1:45661391-45661410 | MS.gene84513:CDS | 30.0% | |
| AGTGTGTACCAACTTTAAAT+GGG | + | chr3.1:45661617-45661636 | MS.gene84513:intron | 30.0% | |
| CTAATCAAAGAACTACATCA+TGG | + | chr3.1:45661385-45661404 | MS.gene84513:CDS | 30.0% | |
| ! | TCAATAACCCCATTTAAAGT+TGG | - | chr3.1:45661628-45661647 | None:intergenic | 30.0% |
| CAGTGTGTACCAACTTTAAA+TGG | + | chr3.1:45661616-45661635 | MS.gene84513:intron | 35.0% | |
| GTGTGTACCAACTTTAAATG+GGG | + | chr3.1:45661618-45661637 | MS.gene84513:intron | 35.0% | |
| !! | ACATTTCTAGCCTCACAATA+AGG | - | chr3.1:45661712-45661731 | None:intergenic | 35.0% |
| AAGTATGTGATGCTGCAGAT+TGG | + | chr3.1:45661658-45661677 | MS.gene84513:CDS | 40.0% | |
| ACATCATGGAATTGGCCAAA+GGG | + | chr3.1:45661399-45661418 | MS.gene84513:CDS | 40.0% | |
| ACCACTTTCTCTTGCAAACA+AGG | - | chr3.1:45661758-45661777 | None:intergenic | 40.0% | |
| AGTATGTGATGCTGCAGATT+GGG | + | chr3.1:45661659-45661678 | MS.gene84513:CDS | 40.0% | |
| CTACTCACCTAGCAGAAAAT+CGG | + | chr3.1:45661490-45661509 | MS.gene84513:CDS | 40.0% | |
| GAAACTGCTTCCTTATTGTG+AGG | + | chr3.1:45661699-45661718 | MS.gene84513:CDS | 40.0% | |
| TACATCATGGAATTGGCCAA+AGG | + | chr3.1:45661398-45661417 | MS.gene84513:CDS | 40.0% | |
| TACTCACCTAGCAGAAAATC+GGG | + | chr3.1:45661491-45661510 | MS.gene84513:CDS | 40.0% | |
| TGCAGATTGGGAAAAGATCA+TGG | + | chr3.1:45661671-45661690 | MS.gene84513:CDS | 40.0% | |
| ! | GATTTTCTGCTAGGTGAGTA+GGG | - | chr3.1:45661491-45661510 | None:intergenic | 40.0% |
| ! | TCCTTGTTTGCAAGAGAAAG+TGG | + | chr3.1:45661754-45661773 | MS.gene84513:CDS | 40.0% |
| AGAGAAAGTGGTCTGCAAGT+AGG | + | chr3.1:45661766-45661785 | MS.gene84513:CDS | 45.0% | |
| TCTTGCAAACAAGGACCAAC+CGG | - | chr3.1:45661749-45661768 | None:intergenic | 45.0% | |
| ! | CGATTTTCTGCTAGGTGAGT+AGG | - | chr3.1:45661492-45661511 | None:intergenic | 45.0% |
| ! | TTACCACCCGATTTTCTGCT+AGG | - | chr3.1:45661500-45661519 | None:intergenic | 45.0% |
| TAGAAATGTTCGCCGCCTCT+CGG | + | chr3.1:45661723-45661742 | MS.gene84513:CDS | 50.0% | |
| TCACCTAGCAGAAAATCGGG+TGG | + | chr3.1:45661494-45661513 | MS.gene84513:CDS | 50.0% | |
| ! | TCCTGAGCTTTAGCTCCCTT+TGG | - | chr3.1:45661417-45661436 | None:intergenic | 50.0% |
| GCCAAAGGGAGCTAAAGCTC+AGG | + | chr3.1:45661413-45661432 | MS.gene84513:CDS | 55.0% | |
| AATGTTCGCCGCCTCTCGGC+CGG | + | chr3.1:45661727-45661746 | MS.gene84513:CDS | 65.0% | |
| ACAAGGACCAACCGGCCGAG+AGG | - | chr3.1:45661741-45661760 | None:intergenic | 65.0% | |
| AGGACCAACCGGCCGAGAGG+CGG | - | chr3.1:45661738-45661757 | None:intergenic | 70.0% | |
| TTCGCCGCCTCTCGGCCGGT+TGG | + | chr3.1:45661731-45661750 | MS.gene84513:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 45661378 | 45661864 | 45661378 | ID=MS.gene84513 |
| chr3.1 | mRNA | 45661378 | 45661864 | 45661378 | ID=MS.gene84513.t1;Parent=MS.gene84513 |
| chr3.1 | exon | 45661378 | 45661515 | 45661378 | ID=MS.gene84513.t1.exon1;Parent=MS.gene84513.t1 |
| chr3.1 | CDS | 45661378 | 45661515 | 45661378 | ID=cds.MS.gene84513.t1;Parent=MS.gene84513.t1 |
| chr3.1 | exon | 45661619 | 45661864 | 45661619 | ID=MS.gene84513.t1.exon2;Parent=MS.gene84513.t1 |
| chr3.1 | CDS | 45661619 | 45661864 | 45661619 | ID=cds.MS.gene84513.t1;Parent=MS.gene84513.t1 |
| Gene Sequence |
| Protein sequence |
