Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84514.t1 | AES70699.1 | 94.5 | 127 | 7 | 0 | 1 | 127 | 1 | 127 | 2.70E-63 | 251.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84514.t1 | Q851V5 | 46.3 | 67 | 30 | 2 | 58 | 122 | 963 | 1025 | 5.5e-09 | 62.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84514.t1 | G8A2Z9 | 94.5 | 127 | 7 | 0 | 1 | 127 | 1 | 127 | 1.9e-63 | 251.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene84514.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84514.t1 | MTR_3g061300 | 94.488 | 127 | 7 | 0 | 1 | 127 | 1 | 127 | 7.89e-88 | 251 |
| MS.gene84514.t1 | MTR_3g061340 | 76.033 | 121 | 29 | 0 | 2 | 122 | 153 | 273 | 2.74e-61 | 189 |
| MS.gene84514.t1 | MTR_3g061370 | 69.421 | 121 | 37 | 0 | 2 | 122 | 160 | 280 | 1.64e-53 | 169 |
| MS.gene84514.t1 | MTR_3g061350 | 67.769 | 121 | 39 | 0 | 2 | 122 | 163 | 283 | 2.24e-51 | 164 |
| MS.gene84514.t1 | MTR_3g061320 | 71.429 | 112 | 32 | 0 | 11 | 122 | 167 | 278 | 1.71e-50 | 162 |
| MS.gene84514.t1 | MTR_3g061310 | 61.789 | 123 | 45 | 1 | 2 | 122 | 162 | 284 | 8.14e-44 | 145 |
| MS.gene84514.t1 | MTR_3g061330 | 66.667 | 90 | 29 | 1 | 2 | 90 | 162 | 251 | 5.90e-34 | 118 |
| MS.gene84514.t1 | MTR_8g103940 | 37.391 | 115 | 62 | 4 | 14 | 122 | 383 | 493 | 1.05e-16 | 75.1 |
| MS.gene84514.t1 | MTR_1g108780 | 38.136 | 118 | 68 | 3 | 14 | 127 | 345 | 461 | 1.09e-15 | 72.4 |
| MS.gene84514.t1 | MTR_1g108780 | 33.636 | 110 | 59 | 6 | 22 | 126 | 192 | 292 | 5.87e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCGTGTTCCAACTTTAAA+TGG | 0.142271 | 3.1:+45673210 | MS.gene84514:intron |
| GATACATTAACTATCACTTT+TGG | 0.350344 | 3.1:-45673442 | None:intergenic |
| CTACTCATTTAGCAGAAAAT+CGG | 0.355466 | 3.1:+45673084 | MS.gene84514:CDS |
| ACATTTCTAGCCTCACAATA+AGG | 0.400255 | 3.1:-45673303 | None:intergenic |
| TCAATAACCCCATTTAAAGT+TGG | 0.400471 | 3.1:-45673219 | None:intergenic |
| TCCTGAGCTCTAGCTCCCTT+TGG | 0.408256 | 3.1:-45673008 | None:intergenic |
| ACTGATAAACAAGAAAGATA+TGG | 0.417726 | 3.1:+45673401 | MS.gene84514:CDS |
| AAAGAACTACATCATGGAAT+TGG | 0.425827 | 3.1:+45672985 | MS.gene84514:CDS |
| AGCGTGTTCCAACTTTAAAT+GGG | 0.429597 | 3.1:+45673211 | MS.gene84514:intron |
| ACCACTTTCTCTTGCAAACA+AGG | 0.468664 | 3.1:-45673349 | None:intergenic |
| TCTTGCAAACAAGGACCAAC+CGG | 0.476293 | 3.1:-45673340 | None:intergenic |
| AATGTTCGCCGCCTCTCGGC+CGG | 0.489655 | 3.1:+45673321 | MS.gene84514:CDS |
| AGTATGTGATGCTGCAGATT+GGG | 0.492313 | 3.1:+45673253 | MS.gene84514:CDS |
| TACTCATTTAGCAGAAAATC+GGG | 0.503743 | 3.1:+45673085 | MS.gene84514:CDS |
| TAGAAATGTTCGCCGCCTCT+CGG | 0.526048 | 3.1:+45673317 | MS.gene84514:CDS |
| TCCTTGTTTGCAAGAGAAAG+TGG | 0.529022 | 3.1:+45673348 | MS.gene84514:CDS |
| ACATCATGGAATTGGCCAAA+GGG | 0.541191 | 3.1:+45672993 | MS.gene84514:CDS |
| AGAGAAAGTGGTCTGCAAGT+AGG | 0.546476 | 3.1:+45673360 | MS.gene84514:CDS |
| GCCAAAGGGAGCTAGAGCTC+AGG | 0.549833 | 3.1:+45673007 | MS.gene84514:CDS |
| TTCGCCGCCTCTCGGCCGGT+TGG | 0.551560 | 3.1:+45673325 | MS.gene84514:CDS |
| TGCAGATTGGGAAAAGATCA+TGG | 0.571416 | 3.1:+45673265 | MS.gene84514:CDS |
| AAGTATGTGATGCTGCAGAT+TGG | 0.577298 | 3.1:+45673252 | MS.gene84514:CDS |
| CTGGTGAAAGAACTACATCA+TGG | 0.579498 | 3.1:+45672979 | MS.gene84514:CDS |
| AGGACCAACCGGCCGAGAGG+CGG | 0.587468 | 3.1:-45673329 | None:intergenic |
| GCAGCTGAGGTTATGAAGAC+TGG | 0.589100 | 3.1:+45672960 | None:intergenic |
| TACATCATGGAATTGGCCAA+AGG | 0.610272 | 3.1:+45672992 | MS.gene84514:CDS |
| GAAACTGCTTCCTTATTGTG+AGG | 0.617392 | 3.1:+45673293 | MS.gene84514:CDS |
| GCGTGTTCCAACTTTAAATG+GGG | 0.619238 | 3.1:+45673212 | MS.gene84514:intron |
| ACAAGGACCAACCGGCCGAG+AGG | 0.636650 | 3.1:-45673332 | None:intergenic |
| TCATTTAGCAGAAAATCGGG+TGG | 0.719217 | 3.1:+45673088 | MS.gene84514:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATGAGAATTGTGAAAAA+AGG | - | chr3.1:45673062-45673081 | None:intergenic | 20.0% |
| ! | ACTGATAAACAAGAAAGATA+TGG | + | chr3.1:45673401-45673420 | MS.gene84514:CDS | 25.0% |
| AAAGAACTACATCATGGAAT+TGG | + | chr3.1:45672985-45673004 | MS.gene84514:CDS | 30.0% | |
| CTACTCATTTAGCAGAAAAT+CGG | + | chr3.1:45673084-45673103 | MS.gene84514:CDS | 30.0% | |
| TACTCATTTAGCAGAAAATC+GGG | + | chr3.1:45673085-45673104 | MS.gene84514:CDS | 30.0% | |
| ! | GATTTTCTGCTAAATGAGTA+GGG | - | chr3.1:45673085-45673104 | None:intergenic | 30.0% |
| ! | TCAATAACCCCATTTAAAGT+TGG | - | chr3.1:45673222-45673241 | None:intergenic | 30.0% |
| AGCGTGTTCCAACTTTAAAT+GGG | + | chr3.1:45673211-45673230 | MS.gene84514:intron | 35.0% | |
| ! | CGATTTTCTGCTAAATGAGT+AGG | - | chr3.1:45673086-45673105 | None:intergenic | 35.0% |
| !! | ACATTTCTAGCCTCACAATA+AGG | - | chr3.1:45673306-45673325 | None:intergenic | 35.0% |
| AAGTATGTGATGCTGCAGAT+TGG | + | chr3.1:45673252-45673271 | MS.gene84514:CDS | 40.0% | |
| ACATCATGGAATTGGCCAAA+GGG | + | chr3.1:45672993-45673012 | MS.gene84514:CDS | 40.0% | |
| ACCACTTTCTCTTGCAAACA+AGG | - | chr3.1:45673352-45673371 | None:intergenic | 40.0% | |
| AGTATGTGATGCTGCAGATT+GGG | + | chr3.1:45673253-45673272 | MS.gene84514:CDS | 40.0% | |
| CAGCGTGTTCCAACTTTAAA+TGG | + | chr3.1:45673210-45673229 | MS.gene84514:intron | 40.0% | |
| CTGGTGAAAGAACTACATCA+TGG | + | chr3.1:45672979-45672998 | MS.gene84514:CDS | 40.0% | |
| GAAACTGCTTCCTTATTGTG+AGG | + | chr3.1:45673293-45673312 | MS.gene84514:CDS | 40.0% | |
| GCGTGTTCCAACTTTAAATG+GGG | + | chr3.1:45673212-45673231 | MS.gene84514:intron | 40.0% | |
| TACATCATGGAATTGGCCAA+AGG | + | chr3.1:45672992-45673011 | MS.gene84514:CDS | 40.0% | |
| TCATTTAGCAGAAAATCGGG+TGG | + | chr3.1:45673088-45673107 | MS.gene84514:CDS | 40.0% | |
| TGCAGATTGGGAAAAGATCA+TGG | + | chr3.1:45673265-45673284 | MS.gene84514:CDS | 40.0% | |
| ! | TCCTTGTTTGCAAGAGAAAG+TGG | + | chr3.1:45673348-45673367 | MS.gene84514:CDS | 40.0% |
| AGAGAAAGTGGTCTGCAAGT+AGG | + | chr3.1:45673360-45673379 | MS.gene84514:CDS | 45.0% | |
| TCTTGCAAACAAGGACCAAC+CGG | - | chr3.1:45673343-45673362 | None:intergenic | 45.0% | |
| TAGAAATGTTCGCCGCCTCT+CGG | + | chr3.1:45673317-45673336 | MS.gene84514:CDS | 50.0% | |
| ! | TCCTGAGCTCTAGCTCCCTT+TGG | - | chr3.1:45673011-45673030 | None:intergenic | 55.0% |
| GCCAAAGGGAGCTAGAGCTC+AGG | + | chr3.1:45673007-45673026 | MS.gene84514:CDS | 60.0% | |
| AATGTTCGCCGCCTCTCGGC+CGG | + | chr3.1:45673321-45673340 | MS.gene84514:CDS | 65.0% | |
| ACAAGGACCAACCGGCCGAG+AGG | - | chr3.1:45673335-45673354 | None:intergenic | 65.0% | |
| AGGACCAACCGGCCGAGAGG+CGG | - | chr3.1:45673332-45673351 | None:intergenic | 70.0% | |
| TTCGCCGCCTCTCGGCCGGT+TGG | + | chr3.1:45673325-45673344 | MS.gene84514:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 45672972 | 45673458 | 45672972 | ID=MS.gene84514 |
| chr3.1 | mRNA | 45672972 | 45673458 | 45672972 | ID=MS.gene84514.t1;Parent=MS.gene84514 |
| chr3.1 | exon | 45672972 | 45673109 | 45672972 | ID=MS.gene84514.t1.exon1;Parent=MS.gene84514.t1 |
| chr3.1 | CDS | 45672972 | 45673109 | 45672972 | ID=cds.MS.gene84514.t1;Parent=MS.gene84514.t1 |
| chr3.1 | exon | 45673213 | 45673458 | 45673213 | ID=MS.gene84514.t1.exon2;Parent=MS.gene84514.t1 |
| chr3.1 | CDS | 45673213 | 45673458 | 45673213 | ID=cds.MS.gene84514.t1;Parent=MS.gene84514.t1 |
| Gene Sequence |
| Protein sequence |
