Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84516.t1 | XP_003600451.1 | 84.2 | 183 | 28 | 1 | 1 | 183 | 25 | 206 | 7.00E-81 | 310.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84516.t1 | Q8L3W1 | 34.5 | 113 | 67 | 1 | 1 | 106 | 21 | 133 | 3.2e-10 | 66.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84516.t1 | G8A2Z6 | 84.2 | 183 | 28 | 1 | 1 | 183 | 25 | 206 | 5.0e-81 | 310.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene84516.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84516.t1 | MTR_3g061330 | 85.246 | 183 | 26 | 1 | 1 | 183 | 25 | 206 | 4.01e-111 | 317 |
| MS.gene84516.t1 | MTR_3g061370 | 81.768 | 181 | 33 | 0 | 1 | 181 | 22 | 202 | 1.70e-106 | 306 |
| MS.gene84516.t1 | MTR_3g061350 | 79.781 | 183 | 37 | 0 | 1 | 183 | 25 | 207 | 6.61e-106 | 305 |
| MS.gene84516.t1 | MTR_3g061320 | 74.317 | 183 | 42 | 3 | 1 | 183 | 25 | 202 | 5.34e-93 | 272 |
| MS.gene84516.t1 | MTR_3g061310 | 69.274 | 179 | 54 | 1 | 1 | 179 | 25 | 202 | 1.14e-84 | 251 |
| MS.gene84516.t1 | MTR_3g061340 | 69.022 | 184 | 45 | 4 | 1 | 183 | 25 | 197 | 1.67e-81 | 243 |
| MS.gene84516.t1 | MTR_3g061290 | 80.147 | 136 | 26 | 1 | 1 | 136 | 25 | 159 | 5.03e-74 | 221 |
| MS.gene84516.t1 | MTR_3g061220 | 65.741 | 108 | 22 | 3 | 1 | 108 | 25 | 117 | 6.85e-41 | 134 |
| MS.gene84516.t1 | MTR_1g035460 | 38.208 | 212 | 101 | 4 | 2 | 183 | 2 | 213 | 6.36e-38 | 134 |
| MS.gene84516.t1 | MTR_1g108780 | 32.692 | 208 | 102 | 3 | 1 | 178 | 12 | 211 | 2.56e-28 | 109 |
| MS.gene84516.t1 | MTR_8g103940 | 32.673 | 202 | 99 | 3 | 1 | 173 | 54 | 247 | 1.21e-26 | 105 |
| MS.gene84516.t1 | MTR_7g050560 | 31.443 | 194 | 97 | 4 | 1 | 178 | 28 | 201 | 3.52e-26 | 102 |
| MS.gene84516.t1 | MTR_7g050720 | 33.503 | 197 | 110 | 4 | 2 | 178 | 26 | 221 | 1.40e-25 | 100 |
| MS.gene84516.t1 | MTR_7g050710 | 48.235 | 85 | 44 | 0 | 1 | 85 | 28 | 112 | 9.70e-25 | 98.2 |
| MS.gene84516.t1 | MTR_5g044670 | 32.796 | 186 | 100 | 4 | 2 | 169 | 32 | 210 | 2.29e-24 | 97.4 |
| MS.gene84516.t1 | MTR_3g098790 | 31.016 | 187 | 125 | 2 | 1 | 183 | 28 | 214 | 1.24e-23 | 95.5 |
| MS.gene84516.t1 | MTR_1g035640 | 34.211 | 190 | 109 | 5 | 1 | 183 | 20 | 200 | 1.56e-23 | 96.7 |
| MS.gene84516.t1 | MTR_7g050590 | 30.244 | 205 | 108 | 4 | 2 | 183 | 2 | 194 | 8.81e-23 | 92.8 |
| MS.gene84516.t1 | MTR_7g050580 | 47.059 | 85 | 45 | 0 | 2 | 86 | 29 | 113 | 1.51e-21 | 89.7 |
| MS.gene84516.t1 | MTR_1g034240 | 39.785 | 93 | 56 | 0 | 1 | 93 | 22 | 114 | 2.02e-19 | 84.7 |
| MS.gene84516.t1 | MTR_4g068320 | 35.897 | 117 | 68 | 1 | 1 | 110 | 21 | 137 | 5.10e-19 | 83.6 |
| MS.gene84516.t1 | MTR_4g119990 | 35.556 | 135 | 80 | 4 | 1 | 134 | 30 | 158 | 3.71e-18 | 80.9 |
| MS.gene84516.t1 | MTR_7g050640 | 45.570 | 79 | 43 | 0 | 1 | 79 | 28 | 106 | 1.34e-17 | 74.7 |
| MS.gene84516.t1 | MTR_1g035620 | 31.788 | 151 | 86 | 4 | 1 | 144 | 24 | 164 | 5.68e-17 | 77.8 |
| MS.gene84516.t1 | MTR_5g070440 | 36.154 | 130 | 79 | 2 | 1 | 128 | 36 | 163 | 6.21e-17 | 76.6 |
| MS.gene84516.t1 | MTR_1g035650 | 43.023 | 86 | 47 | 1 | 1 | 86 | 24 | 107 | 7.16e-17 | 75.1 |
| MS.gene84516.t1 | MTR_1g034210 | 39.785 | 93 | 54 | 1 | 1 | 93 | 22 | 112 | 1.15e-16 | 76.6 |
| MS.gene84516.t1 | MTR_1g034210 | 39.785 | 93 | 54 | 1 | 1 | 93 | 22 | 112 | 1.50e-16 | 76.6 |
| MS.gene84516.t1 | MTR_3g005480 | 40.476 | 84 | 50 | 0 | 1 | 84 | 36 | 119 | 2.58e-16 | 74.7 |
| MS.gene84516.t1 | MTR_1g050845 | 37.374 | 99 | 54 | 2 | 1 | 93 | 24 | 120 | 2.74e-16 | 76.3 |
| MS.gene84516.t1 | MTR_3g005490 | 34.483 | 116 | 65 | 1 | 1 | 105 | 36 | 151 | 6.77e-16 | 73.6 |
| MS.gene84516.t1 | MTR_1g035570 | 33.673 | 98 | 63 | 1 | 1 | 98 | 20 | 115 | 1.40e-15 | 73.9 |
| MS.gene84516.t1 | MTR_1g033740 | 33.600 | 125 | 76 | 4 | 2 | 122 | 38 | 159 | 1.60e-15 | 71.2 |
| MS.gene84516.t1 | MTR_1g035510 | 36.207 | 116 | 66 | 4 | 1 | 112 | 1 | 112 | 1.21e-14 | 69.7 |
| MS.gene84516.t1 | MTR_6g043890 | 29.697 | 165 | 96 | 4 | 1 | 162 | 30 | 177 | 1.52e-14 | 70.5 |
| MS.gene84516.t1 | MTR_6g061070 | 33.133 | 166 | 102 | 5 | 2 | 166 | 34 | 191 | 2.13e-14 | 70.5 |
| MS.gene84516.t1 | MTR_1g035480 | 33.621 | 116 | 69 | 4 | 2 | 112 | 33 | 145 | 6.80e-14 | 66.6 |
| MS.gene84516.t1 | MTR_3g005495 | 39.286 | 84 | 51 | 0 | 1 | 84 | 28 | 111 | 7.86e-14 | 68.9 |
| MS.gene84516.t1 | MTR_3g005495 | 34.400 | 125 | 69 | 2 | 1 | 112 | 258 | 382 | 1.99e-13 | 67.8 |
| MS.gene84516.t1 | MTR_3g005460 | 39.286 | 84 | 51 | 0 | 1 | 84 | 40 | 123 | 1.98e-13 | 67.8 |
| MS.gene84516.t1 | MTR_3g005460 | 28.070 | 171 | 94 | 3 | 1 | 169 | 277 | 420 | 4.09e-12 | 63.9 |
| MS.gene84516.t1 | MTR_1g035470 | 34.783 | 115 | 68 | 3 | 2 | 112 | 2 | 113 | 2.33e-13 | 64.3 |
| MS.gene84516.t1 | MTR_3g005420 | 32.812 | 128 | 86 | 0 | 1 | 128 | 304 | 431 | 2.35e-13 | 67.8 |
| MS.gene84516.t1 | MTR_1g035440 | 38.824 | 85 | 51 | 1 | 1 | 84 | 30 | 114 | 4.07e-13 | 65.9 |
| MS.gene84516.t1 | MTR_3g005440 | 39.286 | 84 | 51 | 0 | 1 | 84 | 35 | 118 | 7.04e-13 | 66.2 |
| MS.gene84516.t1 | MTR_3g005440 | 33.058 | 121 | 79 | 1 | 1 | 119 | 257 | 377 | 4.38e-12 | 63.9 |
| MS.gene84516.t1 | MTR_3g061300 | 60.000 | 45 | 18 | 0 | 139 | 183 | 2 | 46 | 6.93e-12 | 60.1 |
| MS.gene84516.t1 | MTR_1g035500 | 32.787 | 122 | 74 | 4 | 2 | 118 | 41 | 159 | 9.46e-12 | 61.2 |
| MS.gene84516.t1 | MTR_1g035520 | 36.842 | 95 | 57 | 2 | 1 | 95 | 87 | 178 | 3.21e-11 | 61.2 |
| MS.gene84516.t1 | MTR_1g021435 | 25.000 | 184 | 116 | 6 | 2 | 166 | 38 | 218 | 5.14e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84516.t1 | AT1G49475 | 37.903 | 124 | 64 | 3 | 1 | 113 | 49 | 170 | 5.73e-17 | 75.1 |
| MS.gene84516.t1 | AT3G18990 | 34.513 | 113 | 67 | 1 | 1 | 106 | 21 | 133 | 4.78e-15 | 72.0 |
| MS.gene84516.t1 | AT3G18990 | 34.513 | 113 | 67 | 1 | 1 | 106 | 21 | 133 | 7.47e-15 | 71.6 |
| MS.gene84516.t1 | AT4G01580 | 36.697 | 109 | 60 | 3 | 1 | 108 | 45 | 145 | 1.98e-13 | 65.9 |
| MS.gene84516.t1 | AT3G18960 | 36.905 | 84 | 53 | 0 | 1 | 84 | 45 | 128 | 6.64e-13 | 64.7 |
| MS.gene84516.t1 | AT3G18960 | 36.905 | 84 | 53 | 0 | 1 | 84 | 8 | 91 | 7.73e-13 | 63.9 |
| MS.gene84516.t1 | AT3G18960 | 36.905 | 84 | 53 | 0 | 1 | 84 | 45 | 128 | 1.03e-12 | 63.9 |
Find 30 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTTTGATGTACACATATT+TGG | 0.204865 | 3.1:+45760261 | MS.gene84516:CDS |
| TGATGAATCAGTTGAGATTT+TGG | 0.207341 | 3.1:+45760362 | MS.gene84516:CDS |
| CTCTCTTGGACATGGCTCTT+TGG | 0.220059 | 3.1:+45760212 | MS.gene84516:CDS |
| GCACTAAATGGAAAGTTTAT+TGG | 0.220986 | 3.1:+45760130 | MS.gene84516:CDS |
| AGCCTCCTGATGGCACTAAA+TGG | 0.283589 | 3.1:+45760118 | MS.gene84516:CDS |
| TCTTTGGTTGTGTTTGAATA+TGG | 0.291469 | 3.1:+45760228 | MS.gene84516:CDS |
| AATCTATTTCTACAGCATTC+TGG | 0.311518 | 3.1:-45760284 | None:intergenic |
| TGAAATTGTGAGCTACTTCC+TGG | 0.324594 | 3.1:-45760658 | None:intergenic |
| CCAGCTGATGATATACTATA+TGG | 0.344798 | 3.1:-45760721 | None:intergenic |
| TCCTGGGCTCTAGCATCCTT+TGG | 0.368511 | 3.1:-45760641 | None:intergenic |
| AAAGAACTTCATCTATGAAT+TGG | 0.385736 | 3.1:+45760618 | MS.gene84516:CDS |
| TGGTGTGATTTGGTTTGAAA+AGG | 0.433086 | 3.1:+45760164 | MS.gene84516:CDS |
| ACTTTCCATTTAGTGCCATC+AGG | 0.440549 | 3.1:-45760123 | None:intergenic |
| AAATATGTGTACATCAAACT+TGG | 0.477926 | 3.1:-45760259 | None:intergenic |
| AAATACCAAGTAAGTTTACT+AGG | 0.486071 | 3.1:+45760058 | MS.gene84516:CDS |
| TGATTTGGTTTGAAAAGGGT+TGG | 0.498157 | 3.1:+45760169 | MS.gene84516:CDS |
| GGTGTGATTTGGTTTGAAAA+GGG | 0.507291 | 3.1:+45760165 | MS.gene84516:CDS |
| TTTATATGGTCGAGGAGAAA+CGG | 0.524002 | 3.1:-45760427 | None:intergenic |
| TGTCTCCTAGTAAACTTACT+TGG | 0.535374 | 3.1:-45760063 | None:intergenic |
| TCAGGAGGCTTTATCAACAC+TGG | 0.549585 | 3.1:-45760105 | None:intergenic |
| TGAATTGCTTAATCGGAAGA+AGG | 0.555994 | 3.1:+45760386 | MS.gene84516:CDS |
| CCATATAGTATATCATCAGC+TGG | 0.557172 | 3.1:+45760721 | MS.gene84516:CDS |
| GCCAAAGGATGCTAGAGCCC+AGG | 0.562636 | 3.1:+45760640 | MS.gene84516:CDS |
| TTGGTTGTGTTTGAATATGG+AGG | 0.581316 | 3.1:+45760231 | MS.gene84516:CDS |
| TTCATCTATGAATTGGCCAA+AGG | 0.605255 | 3.1:+45760625 | MS.gene84516:CDS |
| TTCCATTTAGTGCCATCAGG+AGG | 0.615384 | 3.1:-45760120 | None:intergenic |
| GAAATTGTGAGCTACTTCCT+GGG | 0.616830 | 3.1:-45760657 | None:intergenic |
| AGTAAGTTTACTAGGAGACA+TGG | 0.620165 | 3.1:+45760066 | MS.gene84516:CDS |
| GTGTTGATAAAGCCTCCTGA+TGG | 0.632454 | 3.1:+45760108 | MS.gene84516:CDS |
| TATGGTCGAGGAGAAACGGA+AGG | 0.680548 | 3.1:-45760423 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTATACTGAAAAGAAAATTA+AGG | - | chr3.1:45760476-45760495 | None:intergenic | 20.0% |
| !! | GTTTATTGGAAAAAAATCAA+TGG | + | chr3.1:45760144-45760163 | MS.gene84516:CDS | 20.0% |
| !! | TTTACTAAAAATTACTCTCT+TGG | + | chr3.1:45760198-45760217 | MS.gene84516:CDS | 20.0% |
| !!! | TTTCTTTTCAGTATACATAA+TGG | + | chr3.1:45760480-45760499 | MS.gene84516:intron | 20.0% |
| ! | AAAAAATCAATGGTGTGATT+TGG | + | chr3.1:45760154-45760173 | MS.gene84516:CDS | 25.0% |
| ! | AAAGAACTTCATCTATGAAT+TGG | + | chr3.1:45760618-45760637 | MS.gene84516:CDS | 25.0% |
| ! | AAATACCAAGTAAGTTTACT+AGG | + | chr3.1:45760058-45760077 | MS.gene84516:CDS | 25.0% |
| ! | AAATATGTGTACATCAAACT+TGG | - | chr3.1:45760262-45760281 | None:intergenic | 25.0% |
| ! | AAGTTTGATGTACACATATT+TGG | + | chr3.1:45760261-45760280 | MS.gene84516:CDS | 25.0% |
| ! | ATATAAAAAAGTTCGAGGTT+TGG | + | chr3.1:45760443-45760462 | MS.gene84516:intron | 25.0% |
| ! | TTCATGAGAATTGTGAAAAA+AGG | - | chr3.1:45760695-45760714 | None:intergenic | 25.0% |
| !! | TTTTGGATGAATTGCTTAAT+CGG | + | chr3.1:45760379-45760398 | MS.gene84516:CDS | 25.0% |
| !!! | AAACCTCGAACTTTTTTATA+TGG | - | chr3.1:45760444-45760463 | None:intergenic | 25.0% |
| AAAAATTACTCTCTTGGACA+TGG | + | chr3.1:45760204-45760223 | MS.gene84516:CDS | 30.0% | |
| GCACTAAATGGAAAGTTTAT+TGG | + | chr3.1:45760130-45760149 | MS.gene84516:CDS | 30.0% | |
| TCTTTGGTTGTGTTTGAATA+TGG | + | chr3.1:45760228-45760247 | MS.gene84516:CDS | 30.0% | |
| ! | TGATGAATCAGTTGAGATTT+TGG | + | chr3.1:45760362-45760381 | MS.gene84516:CDS | 30.0% |
| !! | AATCTATTTCTACAGCATTC+TGG | - | chr3.1:45760287-45760306 | None:intergenic | 30.0% |
| AGTAAGTTTACTAGGAGACA+TGG | + | chr3.1:45760066-45760085 | MS.gene84516:CDS | 35.0% | |
| CGACCATATAAAAAAGTTCG+AGG | + | chr3.1:45760438-45760457 | MS.gene84516:CDS | 35.0% | |
| TACATTGCACACATATATGC+AGG | + | chr3.1:45760575-45760594 | MS.gene84516:intron | 35.0% | |
| TGAATTGCTTAATCGGAAGA+AGG | + | chr3.1:45760386-45760405 | MS.gene84516:CDS | 35.0% | |
| TGTCTCCTAGTAAACTTACT+TGG | - | chr3.1:45760066-45760085 | None:intergenic | 35.0% | |
| TTCATCTATGAATTGGCCAA+AGG | + | chr3.1:45760625-45760644 | MS.gene84516:CDS | 35.0% | |
| TTGGTTGTGTTTGAATATGG+AGG | + | chr3.1:45760231-45760250 | MS.gene84516:CDS | 35.0% | |
| TTTATATGGTCGAGGAGAAA+CGG | - | chr3.1:45760430-45760449 | None:intergenic | 35.0% | |
| ! | AAAAAGTTCGAGGTTTGGTT+TGG | + | chr3.1:45760448-45760467 | MS.gene84516:intron | 35.0% |
| !! | GGTGTGATTTGGTTTGAAAA+GGG | + | chr3.1:45760165-45760184 | MS.gene84516:CDS | 35.0% |
| !! | TGATTTGGTTTGAAAAGGGT+TGG | + | chr3.1:45760169-45760188 | MS.gene84516:CDS | 35.0% |
| !! | TGGTGTGATTTGGTTTGAAA+AGG | + | chr3.1:45760164-45760183 | MS.gene84516:CDS | 35.0% |
| !!! | CGAACTTTTTTATATGGTCG+AGG | - | chr3.1:45760438-45760457 | None:intergenic | 35.0% |
| ACTTTCCATTTAGTGCCATC+AGG | - | chr3.1:45760126-45760145 | None:intergenic | 40.0% | |
| GAAATTGTGAGCTACTTCCT+GGG | - | chr3.1:45760660-45760679 | None:intergenic | 40.0% | |
| TGAAATTGTGAGCTACTTCC+TGG | - | chr3.1:45760661-45760680 | None:intergenic | 40.0% | |
| TGTGTGCAATGTAAAACTCG+TGG | - | chr3.1:45760568-45760587 | None:intergenic | 40.0% | |
| TTCCATTTAGTGCCATCAGG+AGG | - | chr3.1:45760123-45760142 | None:intergenic | 45.0% | |
| ! | GTGTTGATAAAGCCTCCTGA+TGG | + | chr3.1:45760108-45760127 | MS.gene84516:CDS | 45.0% |
| ! | TCAGGAGGCTTTATCAACAC+TGG | - | chr3.1:45760108-45760127 | None:intergenic | 45.0% |
| AGCCTCCTGATGGCACTAAA+TGG | + | chr3.1:45760118-45760137 | MS.gene84516:CDS | 50.0% | |
| CTCTCTTGGACATGGCTCTT+TGG | + | chr3.1:45760212-45760231 | MS.gene84516:CDS | 50.0% | |
| TATGGTCGAGGAGAAACGGA+AGG | - | chr3.1:45760426-45760445 | None:intergenic | 50.0% | |
| ! | TCCTGGGCTCTAGCATCCTT+TGG | - | chr3.1:45760644-45760663 | None:intergenic | 55.0% |
| GCCAAAGGATGCTAGAGCCC+AGG | + | chr3.1:45760640-45760659 | MS.gene84516:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 45760057 | 45760742 | 45760057 | ID=MS.gene84516 |
| chr3.1 | mRNA | 45760057 | 45760742 | 45760057 | ID=MS.gene84516.t1;Parent=MS.gene84516 |
| chr3.1 | exon | 45760057 | 45760459 | 45760057 | ID=MS.gene84516.t1.exon1;Parent=MS.gene84516.t1 |
| chr3.1 | CDS | 45760057 | 45760459 | 45760057 | ID=cds.MS.gene84516.t1;Parent=MS.gene84516.t1 |
| chr3.1 | exon | 45760597 | 45760742 | 45760597 | ID=MS.gene84516.t1.exon2;Parent=MS.gene84516.t1 |
| chr3.1 | CDS | 45760597 | 45760742 | 45760597 | ID=cds.MS.gene84516.t1;Parent=MS.gene84516.t1 |
| Gene Sequence |
| Protein sequence |
