AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84685

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84685.t1 MTR_2g039880 95.051 586 27 1 1 586 1 584 0.0 1095
MS.gene84685.t1 MTR_2g028950 44.802 529 265 10 48 558 45 564 1.20e-143 428
MS.gene84685.t1 MTR_5g090770 40.150 533 293 9 48 563 48 571 2.02e-123 376
MS.gene84685.t1 MTR_2g028950 44.301 465 233 9 48 494 45 501 2.72e-121 368
MS.gene84685.t1 MTR_5g090770 41.463 410 217 7 48 442 48 449 2.18e-95 299
MS.gene84685.t1 MTR_1g051025 31.452 372 211 9 67 402 24 387 2.41e-38 148
MS.gene84685.t1 MTR_7g090020 30.376 372 216 7 67 402 24 388 3.95e-37 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84685.t1 AT1G64280 51.664 571 243 10 7 558 7 563 0.0 550
MS.gene84685.t1 AT4G26120 49.913 575 254 11 10 569 18 573 4.87e-176 511
MS.gene84685.t1 AT4G26120 49.913 575 254 11 10 569 17 572 6.82e-176 511
MS.gene84685.t1 AT5G45110 42.264 530 274 11 48 556 41 559 1.27e-121 372
MS.gene84685.t1 AT4G19660 41.541 532 272 11 48 555 35 551 1.90e-121 370
MS.gene84685.t1 AT4G19660 39.714 559 271 12 48 555 35 578 3.16e-116 358
MS.gene84685.t1 AT5G45110 41.443 485 252 11 48 514 41 511 2.80e-107 332
MS.gene84685.t1 AT3G57130 31.250 368 214 7 67 402 26 386 7.58e-40 152
MS.gene84685.t1 AT2G41370 30.053 376 216 8 67 402 25 393 2.54e-36 142

Find 100 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTGAATTTACTCCGAATT+TGG 0.080716 2.3:+49600719 MS.gene84685:CDS
AGCGAGGAGTAGTTTCTTTA+AGG 0.220140 2.3:+49599198 MS.gene84685:CDS
GCGCTTCACTTGTCGATCTT+CGG 0.290085 2.3:-49601210 None:intergenic
TGAATTGCTTGCTCTTTATC+AGG 0.291540 2.3:+49599465 MS.gene84685:CDS
ACTAGACATTCTTGACAAAA+TGG 0.299539 2.3:+49599768 MS.gene84685:CDS
ATGTTTGTCTAGAAGATTTA+CGG 0.318420 2.3:-49599986 None:intergenic
ATCTAAAGCTCTATGTATTC+TGG 0.325893 2.3:-49600013 None:intergenic
CGCTTCACTTGTCGATCTTC+GGG 0.329375 2.3:-49601209 None:intergenic
GCTACAAGAAGTTCTTAATA+AGG 0.338445 2.3:+49601247 MS.gene84685:CDS
ACTACTGAGCTTCTTGATCT+TGG 0.343475 2.3:+49600141 MS.gene84685:CDS
TCGATTGTGTATCGAGATAT+TGG 0.362910 2.3:+49600383 MS.gene84685:CDS
TCTGAATTTACTCCGAATTT+GGG 0.369283 2.3:+49600720 MS.gene84685:CDS
TCATCTTGAGCAGTGGAGTT+GGG 0.376033 2.3:+49601095 MS.gene84685:intron
AATTCAGATGTGCCATCAAT+TGG 0.381830 2.3:-49600705 None:intergenic
AAAGAGGAGTTTGATAAATC+TGG 0.386748 2.3:+49601284 MS.gene84685:CDS
GAAGTAGTAATAGTTGAAAT+TGG 0.386953 2.3:-49599025 None:intergenic
ATTGTTTACACTTTGGTTGT+TGG 0.395598 2.3:+49599386 MS.gene84685:CDS
GTTCTGCGTTATTTGTATAG+TGG 0.398609 2.3:+49599307 MS.gene84685:CDS
TCATCCTCATCCACGTCAAT+AGG 0.401640 2.3:+49601317 MS.gene84685:CDS
CTCTGGCAACCACCCAAATT+CGG 0.404385 2.3:-49600732 None:intergenic
GTGGCTAAGGATTATGATGT+TGG 0.417096 2.3:+49599265 MS.gene84685:CDS
GCTTCAATACTAGATGATAC+AGG 0.421643 2.3:+49599100 MS.gene84685:CDS
CAAACTCCTCTTTGTCCTCA+TGG 0.421934 2.3:-49601274 None:intergenic
AGAAACTTCTCGACCATCTC+TGG 0.426255 2.3:-49599155 None:intergenic
TAAAAGAGGAGCATCTAATT+CGG 0.429046 2.3:+49600790 MS.gene84685:CDS
TCTCTTTCTCTTGCTAAGGC+AGG 0.429539 2.3:+49600444 MS.gene84685:CDS
CAGATGATGTAGAATTAGTA+AGG 0.438766 2.3:+49600037 MS.gene84685:CDS
CTCTTTCTCTTGCTAAGGCA+GGG 0.442710 2.3:+49600445 MS.gene84685:CDS
TTCTGCGTTATTTGTATAGT+GGG 0.448053 2.3:+49599308 MS.gene84685:CDS
GTCATCTTGAGCAGTGGAGT+TGG 0.451507 2.3:+49601094 MS.gene84685:intron
TATATTACTTGTTTCACTAC+AGG 0.453006 2.3:-49599928 None:intergenic
ATCATACTATTATTAGCCCT+TGG 0.460763 2.3:-49601344 None:intergenic
CCTATCGCAGCTCGCATGCA+TGG 0.465300 2.3:+49601175 MS.gene84685:CDS
GGCATCTCTTTCTCTTGCTA+AGG 0.467473 2.3:+49600440 MS.gene84685:CDS
ATGGATATCAATCCAATTGA+TGG 0.475189 2.3:+49600693 MS.gene84685:CDS
TGGCTAAGGATTATGATGTT+GGG 0.476464 2.3:+49599266 MS.gene84685:CDS
ACGGTTCTTCCCTCGTTGCT+CGG 0.483600 2.3:+49601121 MS.gene84685:CDS
TGAAGCATGAAATCAACAAC+AGG 0.485589 2.3:-49599409 None:intergenic
TTCAGAAATTTGAAAGGTGA+AGG 0.491260 2.3:-49599446 None:intergenic
TGGTTGCCAGAGGACAACTA+TGG 0.504234 2.3:+49600743 MS.gene84685:CDS
ATCACAACCTTTGCTTCCAT+CGG 0.509584 2.3:-49600672 None:intergenic
GAGCAGTGGAGTTGGGGAAA+CGG 0.514130 2.3:+49601102 MS.gene84685:intron
TCTTTCTCTTGCTAAGGCAG+GGG 0.519191 2.3:+49600446 MS.gene84685:CDS
ATTGGAGCAAGCTGAGAGAA+GGG 0.524671 2.3:+49600401 MS.gene84685:CDS
GATGATGATTGTTTACACTT+TGG 0.524715 2.3:+49599379 MS.gene84685:CDS
ATTGTATCTTTGTTAACGAA+GGG 0.525145 2.3:+49600243 MS.gene84685:CDS
GTCGAGAAGTTTCTGTGCAC+CGG 0.525175 2.3:+49599164 MS.gene84685:CDS
TCAAGTCCATAGTTGTCCTC+TGG 0.532702 2.3:-49600749 None:intergenic
GGAAGTGTTGAACAAGATCA+TGG 0.534097 2.3:+49601142 MS.gene84685:CDS
CTATCGCAGCTCGCATGCAT+GGG 0.540766 2.3:+49601176 MS.gene84685:CDS
GTAATGATGTACTTGAGAAG+TGG 0.540852 2.3:+49598932 None:intergenic
TATTGGAGCAAGCTGAGAGA+AGG 0.543360 2.3:+49600400 MS.gene84685:CDS
TATTATAAATCTACTGAGGA+AGG 0.543578 2.3:+49600342 MS.gene84685:CDS
ATGAAGGCGTTGTCTAGAGC+AGG 0.543907 2.3:+49600813 MS.gene84685:CDS
CATTCCTATTGACGTGGATG+AGG 0.544059 2.3:-49601321 None:intergenic
CATCTTGAGCAGTGGAGTTG+GGG 0.545384 2.3:+49601096 MS.gene84685:intron
TAAATCTACTGAGGAAGGAA+AGG 0.549862 2.3:+49600347 MS.gene84685:CDS
GGCACCATTTAAAATAAAAG+AGG 0.551112 2.3:+49600776 MS.gene84685:CDS
TATTGTATCTTTGTTAACGA+AGG 0.551812 2.3:+49600242 MS.gene84685:CDS
ACTTGTTTCACTACAGGTTG+AGG 0.554485 2.3:-49599922 None:intergenic
CCTTCTGAACTCACTTTCGA+TGG 0.555532 2.3:+49600273 MS.gene84685:CDS
GGAGCATCTAATTCGGATGA+AGG 0.559886 2.3:+49600797 MS.gene84685:CDS
TGCGAGCTGCGATAGGTCAT+CGG 0.561459 2.3:-49601168 None:intergenic
GCAACTGAAGAAAGTGGCTA+AGG 0.563471 2.3:+49599252 MS.gene84685:CDS
AGAGATGCCTCACCATGTAA+AGG 0.569360 2.3:-49600426 None:intergenic
CCATGCATGCGAGCTGCGAT+AGG 0.569820 2.3:-49601175 None:intergenic
GAGAGAAGGGAGCCTTTACA+TGG 0.569857 2.3:+49600414 MS.gene84685:CDS
GCAAAGATCATTGCCAGAGA+TGG 0.570514 2.3:+49599142 MS.gene84685:CDS
CCATCGAAAGTGAGTTCAGA+AGG 0.573318 2.3:-49600273 None:intergenic
TAAAGATGTTTGTGTTTGTG+TGG 0.576566 2.3:+49599357 MS.gene84685:CDS
TTTGTTTCCGATGGAAGCAA+AGG 0.576921 2.3:+49600665 MS.gene84685:CDS
AAGCAATTCAGAAATTTGAA+AGG 0.578420 2.3:-49599452 None:intergenic
CTTCAATACTAGATGATACA+GGG 0.581620 2.3:+49599101 MS.gene84685:CDS
GACGCTGCAACTGAAGAAAG+TGG 0.581731 2.3:+49599246 MS.gene84685:CDS
GACAACTATGGACTTGAATG+AGG 0.586182 2.3:+49600755 MS.gene84685:CDS
CGAATTTGGGTGGTTGCCAG+AGG 0.588649 2.3:+49600733 MS.gene84685:CDS
GAATTTACTCCGAATTTGGG+TGG 0.588904 2.3:+49600723 MS.gene84685:CDS
GATGGAAGCAAAGGTTGTGA+TGG 0.592814 2.3:+49600674 MS.gene84685:CDS
CCTTGGCATTCCTATTGACG+TGG 0.593232 2.3:-49601327 None:intergenic
GGCAATAGATGACATGTTGG+TGG 0.595976 2.3:+49599789 MS.gene84685:CDS
CCACGTCAATAGGAATGCCA+AGG 0.605123 2.3:+49601327 MS.gene84685:CDS
ACAGCATAACTCACTAGCAC+TGG 0.609316 2.3:-49598993 None:intergenic
AAGCTAGATTGTCTGAGAGA+CGG 0.610515 2.3:-49599081 None:intergenic
TCCTCGCTGACAGAATGCAC+CGG 0.615150 2.3:-49599183 None:intergenic
AAAGCGTAGTACGAAAGCTG+TGG 0.616351 2.3:+49600317 MS.gene84685:CDS
AAGGGAGCCTTTACATGGTG+AGG 0.619314 2.3:+49600419 MS.gene84685:CDS
AAGATCAAGAAGCTCAGTAG+TGG 0.633228 2.3:-49600139 None:intergenic
GGTCTTCCATGAGGACAAAG+AGG 0.638424 2.3:+49601268 MS.gene84685:CDS
AGTGAAGCGCAGACGATACG+CGG 0.639553 2.3:+49601223 MS.gene84685:CDS
AATGGCAATAGATGACATGT+TGG 0.639924 2.3:+49599786 MS.gene84685:CDS
CACGTCAATAGGAATGCCAA+GGG 0.650742 2.3:+49601328 MS.gene84685:CDS
ATATACAAACGCAAACAACT+CGG 0.651687 2.3:+49599946 MS.gene84685:CDS
AGGACACACTACTCTAGATG+AGG 0.655016 2.3:+49600074 MS.gene84685:CDS
TCTTAATAAGGTCTTCCATG+AGG 0.672788 2.3:+49601259 MS.gene84685:CDS
ACCGGTGCATTCTGTCAGCG+AGG 0.681670 2.3:+49599182 MS.gene84685:CDS
TTGTCTGAGAGACGGTTGAG+TGG 0.683526 2.3:-49599073 None:intergenic
TCAACACTTCCGAGCAACGA+GGG 0.684586 2.3:-49601130 None:intergenic
TTCAACACTTCCGAGCAACG+AGG 0.701823 2.3:-49601131 None:intergenic
GGATTATTATAAATCTACTG+AGG 0.714422 2.3:+49600338 MS.gene84685:CDS
TTGTTGTACCTAGAAAACCG+AGG 0.809311 2.3:+49600486 MS.gene84685:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTTAAAGTAATTTCATA+GGG + chr2.3:49599536-49599555 MS.gene84685:intron 15.0%
!! ATGAAATTAAATTAACTGAA+AGG - chr2.3:49599682-49599701 None:intergenic 15.0%
!! ATGTAAATCATAAAAATCTT+AGG + chr2.3:49600172-49600191 MS.gene84685:CDS 15.0%
!! TCAACATTTATTATGAAATT+CGG - chr2.3:49600603-49600622 None:intergenic 15.0%
!! TGCAAAATTTAACAAATAAA+TGG - chr2.3:49601042-49601061 None:intergenic 15.0%
!! TGTAAATCATAAAAATCTTA+GGG + chr2.3:49600173-49600192 MS.gene84685:CDS 15.0%
!!! AACAAAGATACAATAATTTT+CGG - chr2.3:49600237-49600256 None:intergenic 15.0%
!!! AAGATTTTTATGATTTACAT+CGG - chr2.3:49600172-49600191 None:intergenic 15.0%
!!! TTTTAGAGATTTAAATACAA+TGG - chr2.3:49600918-49600937 None:intergenic 15.0%
!! AAGTTAAAGTAATTTCATAG+GGG + chr2.3:49599537-49599556 MS.gene84685:intron 20.0%
!! CAACATTTATTATGAAATTC+GGG - chr2.3:49600602-49600621 None:intergenic 20.0%
!! GAAAGTTAAAGTAATTTCAT+AGG + chr2.3:49599535-49599554 MS.gene84685:intron 20.0%
!! GTAAAGAAAGAAAAATAGAA+TGG - chr2.3:49600946-49600965 None:intergenic 20.0%
!! GTAAATCATAAAAATCTTAG+GGG + chr2.3:49600174-49600193 MS.gene84685:CDS 20.0%
!! TTATTTGAGAACTTTCTTAA+AGG + chr2.3:49600865-49600884 MS.gene84685:intron 20.0%
!! TTTCATTATACGTTAGATAT+CGG + chr2.3:49601013-49601032 MS.gene84685:intron 20.0%
!!! TTCTTTAAGGATGTTTTTAA+GGG + chr2.3:49599211-49599230 MS.gene84685:CDS 20.0%
!!! TTTCTTTAAGGATGTTTTTA+AGG + chr2.3:49599210-49599229 MS.gene84685:CDS 20.0%
! AAGCAATTCAGAAATTTGAA+AGG - chr2.3:49599455-49599474 None:intergenic 25.0%
! ATCTAACGTATAATGAAAAC+TGG - chr2.3:49601011-49601030 None:intergenic 25.0%
! ATGTTTGTCTAGAAGATTTA+CGG - chr2.3:49599989-49600008 None:intergenic 25.0%
! ATTGTATCTTTGTTAACGAA+GGG + chr2.3:49600243-49600262 MS.gene84685:CDS 25.0%
! GAAGTAGTAATAGTTGAAAT+TGG - chr2.3:49599028-49599047 None:intergenic 25.0%
! GGATTATTATAAATCTACTG+AGG + chr2.3:49600338-49600357 MS.gene84685:CDS 25.0%
! GTTCGAAAAGAATTGATATA+GGG + chr2.3:49600984-49601003 MS.gene84685:intron 25.0%
! TATATTACTTGTTTCACTAC+AGG - chr2.3:49599931-49599950 None:intergenic 25.0%
! TATTATAAATCTACTGAGGA+AGG + chr2.3:49600342-49600361 MS.gene84685:CDS 25.0%
! TATTGTATCTTTGTTAACGA+AGG + chr2.3:49600242-49600261 MS.gene84685:CDS 25.0%
! TCTCAAATAAAACGAAAGAT+CGG - chr2.3:49600855-49600874 None:intergenic 25.0%
! TTTCTTTACTTGTATCTATC+TGG + chr2.3:49600957-49600976 MS.gene84685:intron 25.0%
!!! TGCTCCTCTTTTATTTTAAA+TGG - chr2.3:49600783-49600802 None:intergenic 25.0%
AAAGAGGAGTTTGATAAATC+TGG + chr2.3:49601284-49601303 MS.gene84685:CDS 30.0%
ACTAGACATTCTTGACAAAA+TGG + chr2.3:49599768-49599787 MS.gene84685:CDS 30.0%
ATATACAAACGCAAACAACT+CGG + chr2.3:49599946-49599965 MS.gene84685:CDS 30.0%
ATCATACTATTATTAGCCCT+TGG - chr2.3:49601347-49601366 None:intergenic 30.0%
ATCTGAATTTACTCCGAATT+TGG + chr2.3:49600719-49600738 MS.gene84685:CDS 30.0%
ATGGATATCAATCCAATTGA+TGG + chr2.3:49600693-49600712 MS.gene84685:CDS 30.0%
ATTGTTTACACTTTGGTTGT+TGG + chr2.3:49599386-49599405 MS.gene84685:CDS 30.0%
CAGATGATGTAGAATTAGTA+AGG + chr2.3:49600037-49600056 MS.gene84685:CDS 30.0%
CTTCAATACTAGATGATACA+GGG + chr2.3:49599101-49599120 MS.gene84685:CDS 30.0%
GAACATGTAAGAAAATACCT+CGG - chr2.3:49600506-49600525 None:intergenic 30.0%
GATGATGATTGTTTACACTT+TGG + chr2.3:49599379-49599398 MS.gene84685:CDS 30.0%
GCTACAAGAAGTTCTTAATA+AGG + chr2.3:49601247-49601266 MS.gene84685:CDS 30.0%
GGTTCGAAAAGAATTGATAT+AGG + chr2.3:49600983-49601002 MS.gene84685:intron 30.0%
TAAAGATGTTTGTGTTTGTG+TGG + chr2.3:49599357-49599376 MS.gene84685:CDS 30.0%
TCTGAATTTACTCCGAATTT+GGG + chr2.3:49600720-49600739 MS.gene84685:CDS 30.0%
TTACTTGTATCTATCTGGTT+CGG + chr2.3:49600962-49600981 MS.gene84685:intron 30.0%
TTCAGAAATTTGAAAGGTGA+AGG - chr2.3:49599449-49599468 None:intergenic 30.0%
TTCTGCGTTATTTGTATAGT+GGG + chr2.3:49599308-49599327 MS.gene84685:CDS 30.0%
! ATCTAAAGCTCTATGTATTC+TGG - chr2.3:49600016-49600035 None:intergenic 30.0%
! GGCACCATTTAAAATAAAAG+AGG + chr2.3:49600776-49600795 MS.gene84685:CDS 30.0%
! TTTTCTATGGCAAACATTTG+TGG + chr2.3:49599814-49599833 MS.gene84685:CDS 30.0%
!! ACGATTCATTGTTTTTCAGT+TGG + chr2.3:49600630-49600649 MS.gene84685:intron 30.0%
!! TAAAAGAGGAGCATCTAATT+CGG + chr2.3:49600790-49600809 MS.gene84685:CDS 30.0%
AATGGCAATAGATGACATGT+TGG + chr2.3:49599786-49599805 MS.gene84685:CDS 35.0%
AATTCAGATGTGCCATCAAT+TGG - chr2.3:49600708-49600727 None:intergenic 35.0%
AGAACTTTCTTAAAGGTCGA+AGG + chr2.3:49600872-49600891 MS.gene84685:intron 35.0%
AGCCTCTTGTGTAAAAAACA+GGG - chr2.3:49599636-49599655 None:intergenic 35.0%
GCTTCAATACTAGATGATAC+AGG + chr2.3:49599100-49599119 MS.gene84685:CDS 35.0%
GTTCTGCGTTATTTGTATAG+TGG + chr2.3:49599307-49599326 MS.gene84685:CDS 35.0%
TAAATCTACTGAGGAAGGAA+AGG + chr2.3:49600347-49600366 MS.gene84685:CDS 35.0%
TCGATTGTGTATCGAGATAT+TGG + chr2.3:49600383-49600402 MS.gene84685:CDS 35.0%
TCTTAATAAGGTCTTCCATG+AGG + chr2.3:49601259-49601278 MS.gene84685:CDS 35.0%
TGAAGCATGAAATCAACAAC+AGG - chr2.3:49599412-49599431 None:intergenic 35.0%
TGAATTGCTTGCTCTTTATC+AGG + chr2.3:49599465-49599484 MS.gene84685:CDS 35.0%
TGGCTAAGGATTATGATGTT+GGG + chr2.3:49599266-49599285 MS.gene84685:CDS 35.0%
! AGAAAATACCTCGGTTTTCT+AGG - chr2.3:49600497-49600516 None:intergenic 35.0%
!! AGCAAAACTTTTGTTTCCGA+TGG + chr2.3:49600656-49600675 MS.gene84685:CDS 35.0%
!! CAGTTGATTTGATTACTTGC+AGG + chr2.3:49599738-49599757 MS.gene84685:intron 35.0%
!! CCATTTTGCTTTATTGTACG+CGG + chr2.3:49599605-49599624 MS.gene84685:intron 35.0%
!!! CATGTTGGTGGTTTTTTCTA+TGG + chr2.3:49599801-49599820 MS.gene84685:CDS 35.0%
!!! GTAAGGTTGCTTTTGAAAGA+AGG + chr2.3:49600054-49600073 MS.gene84685:CDS 35.0%
!!! TACCCTGTTTTTTACACAAG+AGG + chr2.3:49599631-49599650 MS.gene84685:intron 35.0%
!!! TTTTTTGTCATCTTGAGCAG+TGG + chr2.3:49601088-49601107 MS.gene84685:intron 35.0%
AAGATCAAGAAGCTCAGTAG+TGG - chr2.3:49600142-49600161 None:intergenic 40.0%
AAGCTAGATTGTCTGAGAGA+CGG - chr2.3:49599084-49599103 None:intergenic 40.0%
ACTACTGAGCTTCTTGATCT+TGG + chr2.3:49600141-49600160 MS.gene84685:CDS 40.0%
ACTTGTTTCACTACAGGTTG+AGG - chr2.3:49599925-49599944 None:intergenic 40.0%
AGCGAGGAGTAGTTTCTTTA+AGG + chr2.3:49599198-49599217 MS.gene84685:CDS 40.0%
ATCACAACCTTTGCTTCCAT+CGG - chr2.3:49600675-49600694 None:intergenic 40.0%
ATTCGGGAGTAACATATGTC+AGG - chr2.3:49600586-49600605 None:intergenic 40.0%
CAGCCTCTTGTGTAAAAAAC+AGG - chr2.3:49599637-49599656 None:intergenic 40.0%
CCGCGTACAATAAAGCAAAA+TGG - chr2.3:49599608-49599627 None:intergenic 40.0%
GAATTTACTCCGAATTTGGG+TGG + chr2.3:49600723-49600742 MS.gene84685:CDS 40.0%
GACAACTATGGACTTGAATG+AGG + chr2.3:49600755-49600774 MS.gene84685:CDS 40.0%
GTGGCTAAGGATTATGATGT+TGG + chr2.3:49599265-49599284 MS.gene84685:CDS 40.0%
TTCGGGAGTAACATATGTCA+GGG - chr2.3:49600585-49600604 None:intergenic 40.0%
TTGTTGTACCTAGAAAACCG+AGG + chr2.3:49600486-49600505 MS.gene84685:CDS 40.0%
TTTGTTTCCGATGGAAGCAA+AGG + chr2.3:49600665-49600684 MS.gene84685:CDS 40.0%
!! GGAAGTGTTGAACAAGATCA+TGG + chr2.3:49601142-49601161 MS.gene84685:CDS 40.0%
!!! AGGCAGTGATTTTTCAAGAG+TGG - chr2.3:49599905-49599924 None:intergenic 40.0%
AAAGCGTAGTACGAAAGCTG+TGG + chr2.3:49600317-49600336 MS.gene84685:CDS 45.0%
ACAGCATAACTCACTAGCAC+TGG - chr2.3:49598996-49599015 None:intergenic 45.0%
AGAAACTTCTCGACCATCTC+TGG - chr2.3:49599158-49599177 None:intergenic 45.0%
AGAGATGCCTCACCATGTAA+AGG - chr2.3:49600429-49600448 None:intergenic 45.0%
AGGACACACTACTCTAGATG+AGG + chr2.3:49600074-49600093 MS.gene84685:CDS 45.0%
ATTGGAGCAAGCTGAGAGAA+GGG + chr2.3:49600401-49600420 MS.gene84685:CDS 45.0%
CAAACTCCTCTTTGTCCTCA+TGG - chr2.3:49601277-49601296 None:intergenic 45.0%
CACGTCAATAGGAATGCCAA+GGG + chr2.3:49601328-49601347 MS.gene84685:CDS 45.0%
CATTCCTATTGACGTGGATG+AGG - chr2.3:49601324-49601343 None:intergenic 45.0%
CCATCGAAAGTGAGTTCAGA+AGG - chr2.3:49600276-49600295 None:intergenic 45.0%
CCTTCTGAACTCACTTTCGA+TGG + chr2.3:49600273-49600292 MS.gene84685:CDS 45.0%
CTCTTTCTCTTGCTAAGGCA+GGG + chr2.3:49600445-49600464 MS.gene84685:CDS 45.0%
GATGGAAGCAAAGGTTGTGA+TGG + chr2.3:49600674-49600693 MS.gene84685:CDS 45.0%
GCAAAGATCATTGCCAGAGA+TGG + chr2.3:49599142-49599161 MS.gene84685:CDS 45.0%
GCAACTGAAGAAAGTGGCTA+AGG + chr2.3:49599252-49599271 MS.gene84685:CDS 45.0%
GGCAATAGATGACATGTTGG+TGG + chr2.3:49599789-49599808 MS.gene84685:CDS 45.0%
GGCATCTCTTTCTCTTGCTA+AGG + chr2.3:49600440-49600459 MS.gene84685:CDS 45.0%
TATTGGAGCAAGCTGAGAGA+AGG + chr2.3:49600400-49600419 MS.gene84685:CDS 45.0%
TCAAGTCCATAGTTGTCCTC+TGG - chr2.3:49600752-49600771 None:intergenic 45.0%
TCATCCTCATCCACGTCAAT+AGG + chr2.3:49601317-49601336 MS.gene84685:CDS 45.0%
TCATCTTGAGCAGTGGAGTT+GGG + chr2.3:49601095-49601114 MS.gene84685:intron 45.0%
TCTCTTTCTCTTGCTAAGGC+AGG + chr2.3:49600444-49600463 MS.gene84685:CDS 45.0%
TCTTTCTCTTGCTAAGGCAG+GGG + chr2.3:49600446-49600465 MS.gene84685:CDS 45.0%
! GGAGCATCTAATTCGGATGA+AGG + chr2.3:49600797-49600816 MS.gene84685:CDS 45.0%
AAAATGGTGAGACCCCATGC+AGG - chr2.3:49599592-49599611 None:intergenic 50.0%
AAGGGAGCCTTTACATGGTG+AGG + chr2.3:49600419-49600438 MS.gene84685:CDS 50.0%
CATCTTGAGCAGTGGAGTTG+GGG + chr2.3:49601096-49601115 MS.gene84685:intron 50.0%
CCACGTCAATAGGAATGCCA+AGG + chr2.3:49601327-49601346 MS.gene84685:CDS 50.0%
CCGTGCACTAAAACTAGGCA+GGG + chr2.3:49599565-49599584 MS.gene84685:intron 50.0%
CCTTGGCATTCCTATTGACG+TGG - chr2.3:49601330-49601349 None:intergenic 50.0%
CGCTTCACTTGTCGATCTTC+GGG - chr2.3:49601212-49601231 None:intergenic 50.0%
CTCTGGCAACCACCCAAATT+CGG - chr2.3:49600735-49600754 None:intergenic 50.0%
GACGCTGCAACTGAAGAAAG+TGG + chr2.3:49599246-49599265 MS.gene84685:CDS 50.0%
GAGAGAAGGGAGCCTTTACA+TGG + chr2.3:49600414-49600433 MS.gene84685:CDS 50.0%
GCGCTTCACTTGTCGATCTT+CGG - chr2.3:49601213-49601232 None:intergenic 50.0%
GGTCTTCCATGAGGACAAAG+AGG + chr2.3:49601268-49601287 MS.gene84685:CDS 50.0%
GTCATCTTGAGCAGTGGAGT+TGG + chr2.3:49601094-49601113 MS.gene84685:intron 50.0%
GTCGAGAAGTTTCTGTGCAC+CGG + chr2.3:49599164-49599183 MS.gene84685:CDS 50.0%
TCAACACTTCCGAGCAACGA+GGG - chr2.3:49601133-49601152 None:intergenic 50.0%
TGGTTGCCAGAGGACAACTA+TGG + chr2.3:49600743-49600762 MS.gene84685:CDS 50.0%
TTCAACACTTCCGAGCAACG+AGG - chr2.3:49601134-49601153 None:intergenic 50.0%
TTGTCTGAGAGACGGTTGAG+TGG - chr2.3:49599076-49599095 None:intergenic 50.0%
!! ATGAAGGCGTTGTCTAGAGC+AGG + chr2.3:49600813-49600832 MS.gene84685:CDS 50.0%
!!! CCCTGCCTAGTTTTAGTGCA+CGG - chr2.3:49599568-49599587 None:intergenic 50.0%
!!! CCTGCCTAGTTTTAGTGCAC+GGG - chr2.3:49599567-49599586 None:intergenic 50.0%
!!! CTGCCTAGTTTTAGTGCACG+GGG - chr2.3:49599566-49599585 None:intergenic 50.0%
AAACTAGGCAGGGTCCTGCA+TGG + chr2.3:49599575-49599594 MS.gene84685:intron 55.0%
AACTAGGCAGGGTCCTGCAT+GGG + chr2.3:49599576-49599595 MS.gene84685:intron 55.0%
ACGGTTCTTCCCTCGTTGCT+CGG + chr2.3:49601121-49601140 MS.gene84685:CDS 55.0%
AGTGAAGCGCAGACGATACG+CGG + chr2.3:49601223-49601242 MS.gene84685:CDS 55.0%
CCCGTGCACTAAAACTAGGC+AGG + chr2.3:49599564-49599583 MS.gene84685:intron 55.0%
CGAATTTGGGTGGTTGCCAG+AGG + chr2.3:49600733-49600752 MS.gene84685:CDS 55.0%
CTATCGCAGCTCGCATGCAT+GGG + chr2.3:49601176-49601195 MS.gene84685:CDS 55.0%
CTGCCCCGTGCACTAAAACT+AGG + chr2.3:49599560-49599579 MS.gene84685:intron 55.0%
GAGCAGTGGAGTTGGGGAAA+CGG + chr2.3:49601102-49601121 MS.gene84685:intron 55.0%
TCCTCGCTGACAGAATGCAC+CGG - chr2.3:49599186-49599205 None:intergenic 55.0%
TGCGAGCTGCGATAGGTCAT+CGG - chr2.3:49601171-49601190 None:intergenic 55.0%
ACCGGTGCATTCTGTCAGCG+AGG + chr2.3:49599182-49599201 MS.gene84685:CDS 60.0%
ACTAGGCAGGGTCCTGCATG+GGG + chr2.3:49599577-49599596 MS.gene84685:intron 60.0%
CCATGCATGCGAGCTGCGAT+AGG - chr2.3:49601178-49601197 None:intergenic 60.0%
CCTATCGCAGCTCGCATGCA+TGG + chr2.3:49601175-49601194 MS.gene84685:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 49598935 49601382 49598935 ID=MS.gene84685
chr2.3 mRNA 49598935 49601382 49598935 ID=MS.gene84685.t1;Parent=MS.gene84685
chr2.3 exon 49598935 49599486 49598935 ID=MS.gene84685.t1.exon1;Parent=MS.gene84685.t1
chr2.3 CDS 49598935 49599486 49598935 ID=cds.MS.gene84685.t1;Parent=MS.gene84685.t1
chr2.3 exon 49599760 49600507 49599760 ID=MS.gene84685.t1.exon2;Parent=MS.gene84685.t1
chr2.3 CDS 49599760 49600507 49599760 ID=cds.MS.gene84685.t1;Parent=MS.gene84685.t1
chr2.3 exon 49600649 49600834 49600649 ID=MS.gene84685.t1.exon3;Parent=MS.gene84685.t1
chr2.3 CDS 49600649 49600834 49600649 ID=cds.MS.gene84685.t1;Parent=MS.gene84685.t1
chr2.3 exon 49601108 49601382 49601108 ID=MS.gene84685.t1.exon4;Parent=MS.gene84685.t1
chr2.3 CDS 49601108 49601382 49601108 ID=cds.MS.gene84685.t1;Parent=MS.gene84685.t1
Gene Sequence

>MS.gene84685

ATGATGTACTTGAGAAGTGGATTCTCTGAATATTCAAACGAAATCAGCAACAACACTGCCAGTGCTAGTGAGTTATGCTGTACAACAGCACCAATTTCAACTATTACTACTTCACAAGATATCATCACAATCACACAACCACTCAACCGTCTCTCAGACAATCTAGCTTCAATACTAGATGATACAGGGTTTGACTTCTTTTCTGACGCAAAGATCATTGCCAGAGATGGTCGAGAAGTTTCTGTGCACCGGTGCATTCTGTCAGCGAGGAGTAGTTTCTTTAAGGATGTTTTTAAGGGAAAGAAAGAAACGACGCTGCAACTGAAGAAAGTGGCTAAGGATTATGATGTTGGGTTTGAAGCGCTTAACGTTGTTCTGCGTTATTTGTATAGTGGGAGAGTTGATAATCATCATTTGTCTGCTAAAGATGTTTGTGTTTGTGTGGATGATGATTGTTTACACTTTGGTTGTTGGCCTGTTGTTGATTTCATGCTTCAACTTCTCTATGTTTCCTTCACCTTTCAAATTTCTGAATTGCTTGCTCTTTATCAGGTAAGAATTAAGCACTTATAGATGCTATTTTAATAATAAAAGATGAAAGAAAGTTAAAGTAATTTCATAGGGGCTGCCCCGTGCACTAAAACTAGGCAGGGTCCTGCATGGGGTCTCACCATTTTGCTTTATTGTACGCGGTCTTACCCTGTTTTTTACACAAGAGGCTGTTTTCGAGACTTGAACTTGTGACCTTTCAGTTAATTTAATTTCATATAGTTTATTAAAATAATCTTAAAACAATTTATTGACAGTTGATTTGATTACTTGCAGGATCATCTACTAGACATTCTTGACAAAATGGCAATAGATGACATGTTGGTGGTTTTTTCTATGGCAAACATTTGTGGTGAAACATGTGATAAACTACTAACAAGATGTACAGAGATAATAGTTGAATCTAATGTTGATATAACCACTCTTGAAAAATCACTGCCTCAACCTGTAGTGAAACAAGTAATATACAAACGCAAACAACTCGGCTTAGACATGTATGAAACCGTAAATCTTCTAGACAAACATGTCACCAGAATACATAGAGCTTTAGATTCAGATGATGTAGAATTAGTAAGGTTGCTTTTGAAAGAAGGACACACTACTCTAGATGAGGCTCATGCACTTCACTATGCTGTTGCTTATTGTGATGTCAAAACCACTACTGAGCTTCTTGATCTTGGACTTGCCGATGTAAATCATAAAAATCTTAGGGGCTATAGCTTGCTACACGTTGCTGCGAAGAGAAAAGAGCCGAAAATTATTGTATCTTTGTTAACGAAGGGAGCTCAACCTTCTGAACTCACTTTCGATGGCAGAAAAGCTCTTCAAATTTCAAAGCGTAGTACGAAAGCTGTGGATTATTATAAATCTACTGAGGAAGGAAAGGTTTCTTCGAATGATCGATTGTGTATCGAGATATTGGAGCAAGCTGAGAGAAGGGAGCCTTTACATGGTGAGGCATCTCTTTCTCTTGCTAAGGCAGGGGATGATTTACGTATGAAATTGTTGTACCTAGAAAACCGAGGTATTTTCTTACATGTTCATATTTTTCGATTTATTTTCTTTTTTTCGATAAATGTTGAATCAACATTTGTGTCGCCCTGACATATGTTACTCCCGAATTTCATAATAAATGTTGATCAACTGACGATTCATTGTTTTTCAGTTGGACTAGCAAAACTTTTGTTTCCGATGGAAGCAAAGGTTGTGATGGATATCAATCCAATTGATGGCACATCTGAATTTACTCCGAATTTGGGTGGTTGCCAGAGGACAACTATGGACTTGAATGAGGCACCATTTAAAATAAAAGAGGAGCATCTAATTCGGATGAAGGCGTTGTCTAGAGCAGGTAATACATACATGTTTCCGATCTTTCGTTTTATTTGAGAACTTTCTTAAAGGTCGAAGGCTCAAAGTAATTCTTATCAACCATTGTATTTAAATCTCTAAAATGTTTCCATTCTATTTTTCTTTCTTTACTTGTATCTATCTGGTTCGGTTCGAAAAGAATTGATATAGGGACCAGTTTTCATTATACGTTAGATATCGGTTACCATTTATTTGTTAAATTTTGCAAATTGATGCTTATATTTTATTTTGATTTTTTTGTCATCTTGAGCAGTGGAGTTGGGGAAACGGTTCTTCCCTCGTTGCTCGGAAGTGTTGAACAAGATCATGGATGCCGATGACCTATCGCAGCTCGCATGCATGGGTCATGACAGTCCCGAAGATCGACAAGTGAAGCGCAGACGATACGCGGAGCTACAAGAAGTTCTTAATAAGGTCTTCCATGAGGACAAAGAGGAGTTTGATAAATCTGGCATGTCTTCATCATCCTCATCCACGTCAATAGGAATGCCAAGGGCTAATAATAGTATGATAGCAATGAAACATTGA
Protein sequence

>MS.gene84685.t1

MMYLRSGFSEYSNEISNNTASASELCCTTAPISTITTSQDIITITQPLNRLSDNLASILDDTGFDFFSDAKIIARDGREVSVHRCILSARSSFFKDVFKGKKETTLQLKKVAKDYDVGFEALNVVLRYLYSGRVDNHHLSAKDVCVCVDDDCLHFGCWPVVDFMLQLLYVSFTFQISELLALYQDHLLDILDKMAIDDMLVVFSMANICGETCDKLLTRCTEIIVESNVDITTLEKSLPQPVVKQVIYKRKQLGLDMYETVNLLDKHVTRIHRALDSDDVELVRLLLKEGHTTLDEAHALHYAVAYCDVKTTTELLDLGLADVNHKNLRGYSLLHVAAKRKEPKIIVSLLTKGAQPSELTFDGRKALQISKRSTKAVDYYKSTEEGKVSSNDRLCIEILEQAERREPLHGEASLSLAKAGDDLRMKLLYLENRVGLAKLLFPMEAKVVMDINPIDGTSEFTPNLGGCQRTTMDLNEAPFKIKEEHLIRMKALSRAVELGKRFFPRCSEVLNKIMDADDLSQLACMGHDSPEDRQVKRRRYAELQEVLNKVFHEDKEEFDKSGMSSSSSSTSIGMPRANNSMIAMKH