AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84716

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84716.t1 MTR_8g024770 94.811 212 11 0 1 212 202 413 5.67e-146 414
MS.gene84716.t1 MTR_8g024790 77.419 217 39 5 1 212 211 422 3.62e-109 321
MS.gene84716.t1 MTR_5g030430 54.037 161 67 2 59 212 474 634 6.65e-47 164
MS.gene84716.t1 MTR_8g067280 50.943 159 71 2 60 211 444 602 1.45e-44 157
MS.gene84716.t1 MTR_7g096350 40.659 182 65 4 27 208 319 457 8.62e-30 115
MS.gene84716.t1 MTR_8g027495 44.118 136 62 5 76 210 456 578 1.73e-24 100
MS.gene84716.t1 MTR_7g117670 80.769 52 10 0 76 127 400 451 3.03e-23 97.4
MS.gene84716.t1 MTR_1g072320 34.459 148 75 3 78 212 470 608 1.53e-16 77.8
MS.gene84716.t1 MTR_0250s0040 29.730 148 80 4 49 191 69 197 1.75e-15 73.2
MS.gene84716.t1 MTR_4g009110 27.152 151 99 3 64 211 164 306 2.64e-15 73.6
MS.gene84716.t1 MTR_3g104100 32.000 150 95 3 66 210 183 330 4.48e-15 73.2
MS.gene84716.t1 MTR_4g009540 29.114 158 90 5 64 211 142 287 1.08e-14 71.6
MS.gene84716.t1 MTR_4g009540 29.114 158 90 5 64 211 142 287 1.21e-14 71.6
MS.gene84716.t1 MTR_4g097940 31.343 134 78 3 64 192 138 262 1.45e-14 71.6
MS.gene84716.t1 MTR_7g083900 60.000 55 22 0 76 130 284 338 2.70e-14 71.2
MS.gene84716.t1 MTR_7g083900 60.000 55 22 0 76 130 284 338 2.78e-14 71.2
MS.gene84716.t1 MTR_3g498695 58.182 55 23 0 76 130 296 350 3.73e-14 70.9
MS.gene84716.t1 MTR_3g498695 58.182 55 23 0 76 130 296 350 3.77e-14 70.9
MS.gene84716.t1 MTR_3g030700 42.697 89 51 0 55 143 173 261 1.09e-13 69.3
MS.gene84716.t1 MTR_4g097950 32.624 141 82 4 53 190 55 185 4.75e-13 66.2
MS.gene84716.t1 MTR_5g014640 34.559 136 71 4 79 210 155 276 6.69e-13 66.6
MS.gene84716.t1 MTR_2g081040 32.624 141 88 3 75 210 174 312 6.70e-13 67.0
MS.gene84716.t1 MTR_4g066460 30.263 152 72 5 45 191 139 261 6.99e-13 66.6
MS.gene84716.t1 MTR_6g047570 30.159 126 74 3 71 191 152 268 7.73e-13 66.6
MS.gene84716.t1 MTR_4g067010 28.788 132 74 4 65 191 137 253 8.99e-13 66.2
MS.gene84716.t1 MTR_8g098275 56.364 55 24 0 77 131 400 454 2.08e-12 65.9
MS.gene84716.t1 MTR_8g098275 56.364 55 24 0 77 131 243 297 2.16e-12 65.5
MS.gene84716.t1 MTR_8g098275 56.364 55 24 0 77 131 307 361 2.24e-12 65.5
MS.gene84716.t1 MTR_4g092700 38.372 86 48 2 46 131 131 211 3.28e-12 64.7
MS.gene84716.t1 MTR_2g038040 50.000 62 24 1 79 133 53 114 3.36e-12 63.9
MS.gene84716.t1 MTR_7g110810 31.624 117 69 2 28 134 153 268 6.76e-12 63.9
MS.gene84716.t1 MTR_0246s0020 28.814 118 75 2 78 190 198 311 7.91e-12 63.9
MS.gene84716.t1 MTR_0246s0020 28.814 118 75 2 78 190 198 311 8.12e-12 63.9
MS.gene84716.t1 MTR_0011s0210 31.624 117 70 3 78 191 183 292 1.08e-11 63.5
MS.gene84716.t1 MTR_2g084280 34.375 96 57 3 82 177 137 226 1.23e-11 63.2
MS.gene84716.t1 MTR_1g112680 50.000 50 25 0 82 131 321 370 2.05e-11 62.8
MS.gene84716.t1 MTR_0011s0260 31.624 117 70 3 78 191 183 292 2.26e-11 62.4
MS.gene84716.t1 MTR_4g057270 52.000 50 24 0 82 131 153 202 2.92e-11 62.0
MS.gene84716.t1 MTR_4g066380 40.476 84 33 2 45 128 137 203 5.04e-11 61.2
MS.gene84716.t1 MTR_4g097920 29.114 158 93 6 57 210 121 263 6.29e-11 60.8
MS.gene84716.t1 MTR_4g097920 29.114 158 93 6 57 210 136 278 6.49e-11 60.8
MS.gene84716.t1 MTR_5g014600 35.366 82 50 1 58 136 126 207 6.57e-11 60.8
MS.gene84716.t1 MTR_5g014520 25.595 168 109 5 30 190 107 265 6.87e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84716.t1 AT1G32640 52.632 171 65 4 48 212 423 583 3.11e-47 164
MS.gene84716.t1 AT4G17880 50.314 159 65 4 59 212 397 546 3.65e-44 155
MS.gene84716.t1 AT5G46760 50.955 157 71 3 59 212 396 549 2.66e-43 153
MS.gene84716.t1 AT4G00870 53.285 137 57 2 77 210 248 380 1.57e-36 132
MS.gene84716.t1 AT5G46830 51.449 138 59 3 76 212 341 471 1.37e-33 125
MS.gene84716.t1 AT2G46510 39.474 190 78 6 28 208 342 503 6.55e-32 121
MS.gene84716.t1 AT1G01260 42.177 147 59 3 62 208 417 537 1.72e-28 112
MS.gene84716.t1 AT1G01260 42.177 147 59 3 62 208 417 537 1.72e-28 112
MS.gene84716.t1 AT1G01260 42.177 147 59 3 62 208 417 537 1.72e-28 112
MS.gene84716.t1 AT4G16430 39.216 153 66 2 58 210 300 425 7.30e-27 107
MS.gene84716.t1 AT4G37850 32.275 189 96 7 24 190 84 262 2.29e-16 76.6
MS.gene84716.t1 AT4G37850 32.275 189 96 7 24 190 84 262 2.29e-16 76.6
MS.gene84716.t1 AT3G26744 60.000 55 22 0 76 130 305 359 5.61e-15 73.2
MS.gene84716.t1 AT3G26744 60.000 55 22 0 76 130 305 359 5.61e-15 73.2
MS.gene84716.t1 AT3G26744 60.000 55 22 0 76 130 305 359 5.61e-15 73.2
MS.gene84716.t1 AT3G26744 60.000 55 22 0 76 130 305 359 5.82e-15 73.2
MS.gene84716.t1 AT1G12860 60.000 55 22 0 76 130 265 319 8.59e-15 72.8
MS.gene84716.t1 AT1G12860 60.000 55 22 0 76 130 265 319 9.24e-15 72.8
MS.gene84716.t1 AT5G57150 36.283 113 50 3 29 141 16 106 2.56e-13 66.6
MS.gene84716.t1 AT4G29930 62.222 45 17 0 79 123 55 99 3.07e-13 66.6
MS.gene84716.t1 AT4G29930 62.222 45 17 0 79 123 55 99 3.14e-13 66.6
MS.gene84716.t1 AT4G29930 62.222 45 17 0 79 123 55 99 3.20e-13 66.6
MS.gene84716.t1 AT4G09820 50.909 55 27 0 77 131 362 416 3.30e-13 68.2
MS.gene84716.t1 AT5G57150 36.283 113 50 3 29 141 16 106 3.38e-13 67.0
MS.gene84716.t1 AT5G57150 40.698 86 34 2 56 141 39 107 3.83e-13 66.2
MS.gene84716.t1 AT5G57150 40.698 86 34 2 56 141 39 107 4.86e-13 66.2
MS.gene84716.t1 AT4G29930 62.222 45 17 0 79 123 55 99 5.80e-13 66.2
MS.gene84716.t1 AT4G29930 62.222 45 17 0 79 123 55 99 5.80e-13 66.2
MS.gene84716.t1 AT4G29930 62.222 45 17 0 79 123 55 99 6.26e-13 66.2
MS.gene84716.t1 AT4G29930 63.636 44 16 0 80 123 56 99 7.84e-13 64.7
MS.gene84716.t1 AT5G57150 40.698 86 34 2 56 141 39 107 8.20e-13 65.9
MS.gene84716.t1 AT1G63650 40.000 70 39 1 78 144 405 474 1.42e-12 66.2
MS.gene84716.t1 AT1G63650 40.000 70 39 1 78 144 405 474 1.42e-12 66.2
MS.gene84716.t1 AT1G63650 40.000 70 39 1 78 144 405 474 1.42e-12 66.2
MS.gene84716.t1 AT2G16910 54.000 50 23 0 82 131 318 367 4.35e-12 65.1
MS.gene84716.t1 AT2G22750 36.842 133 75 4 65 190 137 267 1.29e-11 63.2
MS.gene84716.t1 AT2G22750 36.842 133 75 4 65 190 133 263 1.35e-11 63.2
MS.gene84716.t1 AT2G22750 36.842 133 75 4 65 190 109 239 1.37e-11 62.8
MS.gene84716.t1 AT2G22750 36.842 133 75 4 65 190 137 267 1.43e-11 63.2
MS.gene84716.t1 AT2G22750 36.842 133 75 4 65 190 109 239 1.53e-11 62.8
MS.gene84716.t1 AT5G41315 41.270 63 36 1 69 131 438 499 2.61e-11 62.8
MS.gene84716.t1 AT5G41315 41.270 63 36 1 69 131 433 494 2.67e-11 62.8
MS.gene84716.t1 AT5G41315 41.270 63 36 1 69 131 441 502 2.68e-11 62.8
MS.gene84716.t1 AT1G10610 46.269 67 32 2 79 141 168 234 3.71e-11 62.0
MS.gene84716.t1 AT1G10610 46.269 67 32 2 79 141 235 301 3.78e-11 62.0
MS.gene84716.t1 AT1G10610 46.269 67 32 2 79 141 265 331 4.07e-11 62.0
MS.gene84716.t1 AT4G38070 31.579 114 73 2 19 130 37 147 4.16e-11 61.2
MS.gene84716.t1 AT2G28160 52.000 50 24 0 82 131 135 184 5.66e-11 61.2
MS.gene84716.t1 AT2G28160 52.000 50 24 0 82 131 135 184 5.76e-11 61.2

Find 27 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATTGAAGATTTAAAATT+AGG 0.097958 8.3:+67774720 MS.gene84716:CDS
CTTGAAAGATTTGGAGTTTC+AGG 0.118528 8.3:+67774959 MS.gene84716:CDS
CTTCTCATGGGTGGCTTCTT+TGG 0.173047 8.3:-67774370 None:intergenic
GAGATTGATGTGAAGATTAT+AGG 0.182361 8.3:+67774870 MS.gene84716:CDS
TACTACAACTAGGTTTGTTT+TGG 0.270219 8.3:-67774830 None:intergenic
ATCTTAAAGCATAGAATCTA+TGG 0.304209 8.3:-67774617 None:intergenic
AGATTGAAGATTTAAAATTA+GGG 0.310294 8.3:+67774721 MS.gene84716:CDS
TTATCCATCTTTGAAACGTT+TGG 0.324231 8.3:-67774648 None:intergenic
GCCAACATGGGTGTCGTCTC+TGG 0.337682 8.3:+67774339 None:intergenic
AATGAGTGTCTTGAAAGATT+TGG 0.347886 8.3:+67774950 MS.gene84716:CDS
TTTATCTCTCCTTTGTCTCT+CGG 0.386285 8.3:-67774589 None:intergenic
ATGGGTGGCTTCTTTGGCGC+TGG 0.396457 8.3:-67774364 None:intergenic
GGTTCCAAACGTTTCAAAGA+TGG 0.424360 8.3:+67774644 MS.gene84716:CDS
TTTAAAATTAGGGCAACAAA+AGG 0.456499 8.3:+67774731 MS.gene84716:CDS
AAAAGAGAACCGAAGAAAAG+AGG 0.478932 8.3:+67774519 MS.gene84716:CDS
TCGGCTTCCACATGGTTCAT+CGG 0.486588 8.3:-67774570 None:intergenic
GGTGTTTGAGTTCGTGTCAA+CGG 0.501817 8.3:-67774549 None:intergenic
ATTCTATGCTTTAAGATCTG+TGG 0.527981 8.3:+67774623 MS.gene84716:CDS
ACACTCATTAATCGTGCACT+AGG 0.531276 8.3:-67774936 None:intergenic
TTCTTTGTTATCAGATGCAG+TGG 0.539939 8.3:+67774674 MS.gene84716:CDS
TTTGTCTCTCGGCTTCCACA+TGG 0.548386 8.3:-67774578 None:intergenic
ATGTGGAAGCCGAGAGACAA+AGG 0.579932 8.3:+67774580 MS.gene84716:CDS
ATAAGATTCTTACTACAACT+AGG 0.600771 8.3:-67774840 None:intergenic
GATTATAGGACATGATGCTA+TGG 0.615350 8.3:+67774884 MS.gene84716:CDS
CACTCATTAATCGTGCACTA+GGG 0.626623 8.3:-67774935 None:intergenic
TGCAACGACAACTTCAATCG+TGG 0.678130 8.3:+67774803 MS.gene84716:CDS
TCAAACACCGATGAACCATG+TGG 0.723026 8.3:+67774563 MS.gene84716:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGATTGAAGATTTAAAATT+AGG + chr8.3:67774720-67774739 MS.gene84716:CDS 15.0%
!!! AGATTGAAGATTTAAAATTA+GGG + chr8.3:67774721-67774740 MS.gene84716:CDS 15.0%
! ATAAGATTCTTACTACAACT+AGG - chr8.3:67774843-67774862 None:intergenic 25.0%
! ATCTTAAAGCATAGAATCTA+TGG - chr8.3:67774620-67774639 None:intergenic 25.0%
!! TTTAAAATTAGGGCAACAAA+AGG + chr8.3:67774731-67774750 MS.gene84716:CDS 25.0%
!!! CAATTTTTTTTGCTTCTCAT+GGG - chr8.3:67774385-67774404 None:intergenic 25.0%
AATGAGTGTCTTGAAAGATT+TGG + chr8.3:67774950-67774969 MS.gene84716:CDS 30.0%
GAGATTGATGTGAAGATTAT+AGG + chr8.3:67774870-67774889 MS.gene84716:CDS 30.0%
!! ATTCTATGCTTTAAGATCTG+TGG + chr8.3:67774623-67774642 MS.gene84716:CDS 30.0%
!! TACTACAACTAGGTTTGTTT+TGG - chr8.3:67774833-67774852 None:intergenic 30.0%
!! TTATCCATCTTTGAAACGTT+TGG - chr8.3:67774651-67774670 None:intergenic 30.0%
!!! GCAATTTTTTTTGCTTCTCA+TGG - chr8.3:67774386-67774405 None:intergenic 30.0%
!!! GTTGTTTTTGTTGCTAATAG+TGG - chr8.3:67774474-67774493 None:intergenic 30.0%
AAAAGAGAACCGAAGAAAAG+AGG + chr8.3:67774519-67774538 MS.gene84716:CDS 35.0%
GATTATAGGACATGATGCTA+TGG + chr8.3:67774884-67774903 MS.gene84716:CDS 35.0%
TTTATCTCTCCTTTGTCTCT+CGG - chr8.3:67774592-67774611 None:intergenic 35.0%
! GGTTTTCTTCCTCTTTTCTT+CGG - chr8.3:67774531-67774550 None:intergenic 35.0%
! TTCTTTGTTATCAGATGCAG+TGG + chr8.3:67774674-67774693 MS.gene84716:CDS 35.0%
!!! TTTTTTTTGCTTCTCATGGG+TGG - chr8.3:67774382-67774401 None:intergenic 35.0%
ACACTCATTAATCGTGCACT+AGG - chr8.3:67774939-67774958 None:intergenic 40.0%
CACTCATTAATCGTGCACTA+GGG - chr8.3:67774938-67774957 None:intergenic 40.0%
GGTTCCAAACGTTTCAAAGA+TGG + chr8.3:67774644-67774663 MS.gene84716:CDS 40.0%
TCAAACACCGATGAACCATG+TGG + chr8.3:67774563-67774582 MS.gene84716:CDS 45.0%
TGCAACGACAACTTCAATCG+TGG + chr8.3:67774803-67774822 MS.gene84716:CDS 45.0%
! GGTGTTTGAGTTCGTGTCAA+CGG - chr8.3:67774552-67774571 None:intergenic 45.0%
ATGTGGAAGCCGAGAGACAA+AGG + chr8.3:67774580-67774599 MS.gene84716:CDS 50.0%
CTTCTCATGGGTGGCTTCTT+TGG - chr8.3:67774373-67774392 None:intergenic 50.0%
TCGGCTTCCACATGGTTCAT+CGG - chr8.3:67774573-67774592 None:intergenic 50.0%
TTTGTCTCTCGGCTTCCACA+TGG - chr8.3:67774581-67774600 None:intergenic 50.0%
ATGGGTGGCTTCTTTGGCGC+TGG - chr8.3:67774367-67774386 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 67774345 67774980 67774345 ID=MS.gene84716
chr8.3 mRNA 67774345 67774980 67774345 ID=MS.gene84716.t1;Parent=MS.gene84716
chr8.3 exon 67774345 67774980 67774345 ID=MS.gene84716.t1.exon1;Parent=MS.gene84716.t1
chr8.3 CDS 67774345 67774980 67774345 ID=cds.MS.gene84716.t1;Parent=MS.gene84716.t1
Gene Sequence

>MS.gene84716

ATGGGTGTCGTCTCTGGCGCCAGCGCCAAAGAAGCCACCCATGAGAAGCAAAAAAAATTGCAAGAAAAACATAAAAATGTTTCATCATGTTCTTTAGATTCTGAAGATTCTGATTCAGAATATTGTCCACTATTAGCAACAAAAACAACAACGACAAAGAACGATTCTTTTGAGAAAAGAGAACCGAAGAAAAGAGGAAGAAAACCGTTGACACGAACTCAAACACCGATGAACCATGTGGAAGCCGAGAGACAAAGGAGAGATAAACTAAACCATAGATTCTATGCTTTAAGATCTGTGGTTCCAAACGTTTCAAAGATGGATAAAGCTTCTTTGTTATCAGATGCAGTGGATTACATCAATGAGTTAAAAGCAAAGATTGAAGATTTAAAATTAGGGCAACAAAAGGAATCGAAAAAGTTGATGATGAAAACGATGAAAATTGTCGATAATAACAGTGCAACGACAACTTCAATCGTGGTAGACCAAAACAAACCTAGTTGTAGTAAGAATCTTATTGCTTTAGAGATTGATGTGAAGATTATAGGACATGATGCTATGGTGAGAGTTCAAAGTTTGAATGTTAATCACCCTAGTGCACGATTAATGAGTGTCTTGAAAGATTTGGAGTTTCAG
Protein sequence

>MS.gene84716.t1

MGVVSGASAKEATHEKQKKLQEKHKNVSSCSLDSEDSDSEYCPLLATKTTTTKNDSFEKREPKKRGRKPLTRTQTPMNHVEAERQRRDKLNHRFYALRSVVPNVSKMDKASLLSDAVDYINELKAKIEDLKLGQQKESKKLMMKTMKIVDNNSATTTSIVVDQNKPSCSKNLIALEIDVKIIGHDAMVRVQSLNVNHPSARLMSVLKDLEFQ