Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84932.t1 | RHN79225.1 | 71.7 | 315 | 57 | 4 | 1 | 312 | 1 | 286 | 3.60E-85 | 325.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84932.t1 | A0A396JSM8 | 71.7 | 315 | 57 | 4 | 1 | 312 | 1 | 286 | 2.6e-85 | 325.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene84932.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84932.t1 | MTR_1g053530 | 53.030 | 264 | 87 | 10 | 17 | 275 | 16 | 247 | 1.55e-69 | 216 |
| MS.gene84932.t1 | MTR_1g046810 | 31.902 | 326 | 134 | 12 | 26 | 310 | 37 | 315 | 2.85e-30 | 117 |
| MS.gene84932.t1 | MTR_1g052185 | 31.210 | 314 | 160 | 14 | 5 | 308 | 11 | 278 | 1.72e-22 | 95.1 |
| MS.gene84932.t1 | MTR_4g073310 | 26.885 | 305 | 135 | 7 | 5 | 309 | 11 | 227 | 8.65e-13 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 60 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAATTCCAGCTCCGTTTCT+TGG | 0.269877 | 1.3:+37590430 | None:intergenic |
| GAAGAAAGTTGTTGCCTCTA+TGG | 0.295075 | 1.3:+37590678 | None:intergenic |
| AGAGGCAACAACTTTCTTCT+AGG | 0.323367 | 1.3:-37590674 | MS.gene84932:CDS |
| TGAGGAACCAACTCATGAAT+TGG | 0.350528 | 1.3:-37589988 | MS.gene84932:CDS |
| ACTCTCATCACAAACCAAAA+AGG | 0.357704 | 1.3:-37590793 | MS.gene84932:CDS |
| GTTCTCTTCATTATCATCAT+TGG | 0.362989 | 1.3:+37590115 | None:intergenic |
| TCTTCGAAGTCGAACCATAT+TGG | 0.367921 | 1.3:+37590531 | None:intergenic |
| ATGAAATCGCACAGCCAATA+TGG | 0.368027 | 1.3:-37590545 | MS.gene84932:CDS |
| TCATCTTCACCAGTGTTGTT+TGG | 0.374435 | 1.3:+37590020 | None:intergenic |
| TCATCTTCACCAGTGTTGTT+TGG | 0.380635 | 1.3:+37590056 | None:intergenic |
| ATCATTGGAAGCAGATTGAT+TGG | 0.381296 | 1.3:+37590130 | None:intergenic |
| GAGGAACCAACTCATGAATT+GGG | 0.382420 | 1.3:-37589987 | MS.gene84932:CDS |
| AAATACTTTCTCAAATCAAT+TGG | 0.400225 | 1.3:+37590473 | None:intergenic |
| TGATTCCCAACAAGGTTGTT+AGG | 0.405927 | 1.3:+37590350 | None:intergenic |
| TGCAGAAATGTGGGTGGGAA+TGG | 0.425504 | 1.3:-37590302 | MS.gene84932:CDS |
| CGATCACCTAACAACCTTGT+TGG | 0.429642 | 1.3:-37590356 | MS.gene84932:CDS |
| TGCCTCTATGGAATGCTTTG+TGG | 0.438332 | 1.3:+37590690 | None:intergenic |
| TCATGTTGCTGAAGCAATGC+TGG | 0.444801 | 1.3:-37590390 | MS.gene84932:CDS |
| GATCACCTAACAACCTTGTT+GGG | 0.460149 | 1.3:-37590355 | MS.gene84932:CDS |
| TTCTTGGTCACATAACTCAT+TGG | 0.463818 | 1.3:+37590446 | None:intergenic |
| CAATTGGTGGAAATTGATCT+AGG | 0.480999 | 1.3:+37590489 | None:intergenic |
| TACTTTCTCAAATCAATTGG+TGG | 0.486384 | 1.3:+37590476 | None:intergenic |
| GCAGAAATGTGGGTGGGAAT+GGG | 0.488317 | 1.3:-37590301 | MS.gene84932:CDS |
| GAATGCTTTGTGGCCGCCGA+GGG | 0.493083 | 1.3:+37590700 | None:intergenic |
| TTTAACCCCAATTCATGAGT+TGG | 0.505846 | 1.3:+37589981 | None:intergenic |
| GGAATCACCGTGTCACGAAA+AGG | 0.512728 | 1.3:-37590334 | MS.gene84932:CDS |
| GATCAAACTTTGCAGAAATG+TGG | 0.524856 | 1.3:-37590312 | MS.gene84932:CDS |
| ATATGGTTCGACTTCGAAGA+AGG | 0.528018 | 1.3:-37590528 | MS.gene84932:CDS |
| TTGGTCACATAACTCATTGG+TGG | 0.531076 | 1.3:+37590449 | None:intergenic |
| TTCACCACTGGTAACGCCCT+CGG | 0.552306 | 1.3:-37590716 | MS.gene84932:CDS |
| ATCAAACTTTGCAGAAATGT+GGG | 0.554714 | 1.3:-37590311 | MS.gene84932:CDS |
| TGAGTTATGTGACCAAGAAA+CGG | 0.560971 | 1.3:-37590442 | MS.gene84932:CDS |
| AAAAGGAGTGTTGTTGATCA+TGG | 0.562216 | 1.3:-37590776 | MS.gene84932:CDS |
| AAAAGAACAGCTTGGTGGTG+AGG | 0.570897 | 1.3:-37590196 | MS.gene84932:CDS |
| TCATCTGTCAATGCTCTCAA+CGG | 0.575258 | 1.3:-37590572 | MS.gene84932:CDS |
| TCATCTGCATCATCATCCAC+TGG | 0.575486 | 1.3:+37589927 | None:intergenic |
| AAACAATGAAAAGAACAGCT+TGG | 0.582230 | 1.3:-37590204 | MS.gene84932:CDS |
| CAATGAAAAGAACAGCTTGG+TGG | 0.584869 | 1.3:-37590201 | MS.gene84932:CDS |
| AACTTTGCAGAAATGTGGGT+GGG | 0.585412 | 1.3:-37590307 | MS.gene84932:CDS |
| GGAATGCTTTGTGGCCGCCG+AGG | 0.588007 | 1.3:+37590699 | None:intergenic |
| GCAACGAACACATGAATGGT+TGG | 0.590907 | 1.3:+37590607 | None:intergenic |
| TGAAGATGATGAAGCAAATG+AGG | 0.593094 | 1.3:-37590006 | MS.gene84932:CDS |
| CGATTTGAATGAGCTGCCAG+TGG | 0.597243 | 1.3:-37589943 | MS.gene84932:CDS |
| ATGTGACCAAGAAACGGAGC+TGG | 0.597485 | 1.3:-37590436 | MS.gene84932:CDS |
| AAGATGAAGATCAGAGAGCA+AGG | 0.598147 | 1.3:-37590278 | MS.gene84932:CDS |
| TATGGTTCGACTTCGAAGAA+GGG | 0.600043 | 1.3:-37590527 | MS.gene84932:CDS |
| GCCGCCGAGGGCGTTACCAG+TGG | 0.610498 | 1.3:+37590712 | None:intergenic |
| TGTGACAGAGAATTCACCAC+TGG | 0.614086 | 1.3:-37590728 | MS.gene84932:CDS |
| TGACACGGTGATTCCCAACA+AGG | 0.616814 | 1.3:+37590342 | None:intergenic |
| AAACTTTGCAGAAATGTGGG+TGG | 0.628923 | 1.3:-37590308 | MS.gene84932:CDS |
| AGGAACCAACTCATGAATTG+GGG | 0.629510 | 1.3:-37589986 | MS.gene84932:CDS |
| GGCCACAAAGCATTCCATAG+AGG | 0.645608 | 1.3:-37590692 | MS.gene84932:CDS |
| GACGAGGAACCAAACAACAC+TGG | 0.652317 | 1.3:-37590065 | MS.gene84932:CDS |
| TGCTGCAACGAACACATGAA+TGG | 0.655011 | 1.3:+37590603 | None:intergenic |
| AATGAAGAGAACAACAACAC+TGG | 0.663304 | 1.3:-37590104 | MS.gene84932:CDS |
| GTGAGGTTAAAGATTCCGAA+TGG | 0.667579 | 1.3:-37590179 | MS.gene84932:CDS |
| GATGAGGAACCAAACAACAC+TGG | 0.675447 | 1.3:-37590029 | MS.gene84932:CDS |
| ACCACTGGTAACGCCCTCGG+CGG | 0.680554 | 1.3:-37590713 | MS.gene84932:CDS |
| TGGTGAAGATGATGTAGATG+AGG | 0.685852 | 1.3:-37590045 | MS.gene84932:CDS |
| TGAAGATGATGAAACAGACG+AGG | 0.739030 | 1.3:-37590081 | MS.gene84932:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATACTTTCTCAAATCAAT+TGG | + | chr1.3:37590274-37590293 | None:intergenic | 20.0% |
| AAACAATGAAAAGAACAGCT+TGG | - | chr1.3:37590540-37590559 | MS.gene84932:CDS | 30.0% | |
| AACAAAGAAAAACATGCTGA+TGG | - | chr1.3:37590100-37590119 | MS.gene84932:CDS | 30.0% | |
| ATCAAACTTTGCAGAAATGT+GGG | - | chr1.3:37590433-37590452 | MS.gene84932:CDS | 30.0% | |
| GTTCTCTTCATTATCATCAT+TGG | + | chr1.3:37590632-37590651 | None:intergenic | 30.0% | |
| TACTTTCTCAAATCAATTGG+TGG | + | chr1.3:37590271-37590290 | None:intergenic | 30.0% | |
| ! | CTTCTGATTTTTGAATGACA+AGG | + | chr1.3:37590591-37590610 | None:intergenic | 30.0% |
| AATGAAGAGAACAACAACAC+TGG | - | chr1.3:37590640-37590659 | MS.gene84932:CDS | 35.0% | |
| ACTCTCATCACAAACCAAAA+AGG | - | chr1.3:37589951-37589970 | MS.gene84932:CDS | 35.0% | |
| ATCATTGGAAGCAGATTGAT+TGG | + | chr1.3:37590617-37590636 | None:intergenic | 35.0% | |
| CAATTGGTGGAAATTGATCT+AGG | + | chr1.3:37590258-37590277 | None:intergenic | 35.0% | |
| GATCAAACTTTGCAGAAATG+TGG | - | chr1.3:37590432-37590451 | MS.gene84932:CDS | 35.0% | |
| TGAAGATGATGAAGCAAATG+AGG | - | chr1.3:37590738-37590757 | MS.gene84932:CDS | 35.0% | |
| TGAGTTATGTGACCAAGAAA+CGG | - | chr1.3:37590302-37590321 | MS.gene84932:CDS | 35.0% | |
| TTCTTGGTCACATAACTCAT+TGG | + | chr1.3:37590301-37590320 | None:intergenic | 35.0% | |
| TTTAACCCCAATTCATGAGT+TGG | + | chr1.3:37590766-37590785 | None:intergenic | 35.0% | |
| ! | TGATCAACAACACTCCTTTT+TGG | + | chr1.3:37589968-37589987 | None:intergenic | 35.0% |
| ! | TTTTGAATGACAAGGCCATT+CGG | + | chr1.3:37590583-37590602 | None:intergenic | 35.0% |
| !! | AAAAGGAGTGTTGTTGATCA+TGG | - | chr1.3:37589968-37589987 | MS.gene84932:CDS | 35.0% |
| AAACTTTGCAGAAATGTGGG+TGG | - | chr1.3:37590436-37590455 | MS.gene84932:CDS | 40.0% | |
| AACTTTGCAGAAATGTGGGT+GGG | - | chr1.3:37590437-37590456 | MS.gene84932:CDS | 40.0% | |
| AAGATGAAGATCAGAGAGCA+AGG | - | chr1.3:37590466-37590485 | MS.gene84932:CDS | 40.0% | |
| AGAGGCAACAACTTTCTTCT+AGG | - | chr1.3:37590070-37590089 | MS.gene84932:CDS | 40.0% | |
| AGGAACCAACTCATGAATTG+GGG | - | chr1.3:37590758-37590777 | MS.gene84932:CDS | 40.0% | |
| ATAATTCCAGCTCCGTTTCT+TGG | + | chr1.3:37590317-37590336 | None:intergenic | 40.0% | |
| ATATGGTTCGACTTCGAAGA+AGG | - | chr1.3:37590216-37590235 | MS.gene84932:CDS | 40.0% | |
| ATGAAATCGCACAGCCAATA+TGG | - | chr1.3:37590199-37590218 | MS.gene84932:CDS | 40.0% | |
| CAATGAAAAGAACAGCTTGG+TGG | - | chr1.3:37590543-37590562 | MS.gene84932:CDS | 40.0% | |
| GAAGAAAGTTGTTGCCTCTA+TGG | + | chr1.3:37590069-37590088 | None:intergenic | 40.0% | |
| GAGGAACCAACTCATGAATT+GGG | - | chr1.3:37590757-37590776 | MS.gene84932:CDS | 40.0% | |
| GATCACCTAACAACCTTGTT+GGG | - | chr1.3:37590389-37590408 | MS.gene84932:CDS | 40.0% | |
| GTGAGGTTAAAGATTCCGAA+TGG | - | chr1.3:37590565-37590584 | MS.gene84932:CDS | 40.0% | |
| TATGGTTCGACTTCGAAGAA+GGG | - | chr1.3:37590217-37590236 | MS.gene84932:CDS | 40.0% | |
| TCATCTGTCAATGCTCTCAA+CGG | - | chr1.3:37590172-37590191 | MS.gene84932:CDS | 40.0% | |
| TCTTCGAAGTCGAACCATAT+TGG | + | chr1.3:37590216-37590235 | None:intergenic | 40.0% | |
| TGAAGATGATGAAACAGACG+AGG | - | chr1.3:37590663-37590682 | MS.gene84932:CDS | 40.0% | |
| TGAGGAACCAACTCATGAAT+TGG | - | chr1.3:37590756-37590775 | MS.gene84932:CDS | 40.0% | |
| TGATTCCCAACAAGGTTGTT+AGG | + | chr1.3:37590397-37590416 | None:intergenic | 40.0% | |
| TGGTGAAGATGATGTAGATG+AGG | - | chr1.3:37590699-37590718 | MS.gene84932:CDS | 40.0% | |
| TTGGTCACATAACTCATTGG+TGG | + | chr1.3:37590298-37590317 | None:intergenic | 40.0% | |
| ! | GTTTGATCCTTTTCGTGACA+CGG | + | chr1.3:37590420-37590439 | None:intergenic | 40.0% |
| ! | TCATCTTCACCAGTGTTGTT+TGG | + | chr1.3:37590652-37590671 | None:intergenic | 40.0% |
| CGATCACCTAACAACCTTGT+TGG | - | chr1.3:37590388-37590407 | MS.gene84932:CDS | 45.0% | |
| GATGAGGAACCAAACAACAC+TGG | - | chr1.3:37590715-37590734 | MS.gene84932:CDS | 45.0% | |
| GCAACGAACACATGAATGGT+TGG | + | chr1.3:37590140-37590159 | None:intergenic | 45.0% | |
| TCATCTGCATCATCATCCAC+TGG | + | chr1.3:37590820-37590839 | None:intergenic | 45.0% | |
| TCATGTTGCTGAAGCAATGC+TGG | - | chr1.3:37590354-37590373 | MS.gene84932:CDS | 45.0% | |
| TGCCTCTATGGAATGCTTTG+TGG | + | chr1.3:37590057-37590076 | None:intergenic | 45.0% | |
| TGCTGCAACGAACACATGAA+TGG | + | chr1.3:37590144-37590163 | None:intergenic | 45.0% | |
| TGTGACAGAGAATTCACCAC+TGG | - | chr1.3:37590016-37590035 | MS.gene84932:CDS | 45.0% | |
| !! | AAAAGAACAGCTTGGTGGTG+AGG | - | chr1.3:37590548-37590567 | MS.gene84932:CDS | 45.0% |
| ATGTGACCAAGAAACGGAGC+TGG | - | chr1.3:37590308-37590327 | MS.gene84932:CDS | 50.0% | |
| CGATTTGAATGAGCTGCCAG+TGG | - | chr1.3:37590801-37590820 | MS.gene84932:CDS | 50.0% | |
| GACGAGGAACCAAACAACAC+TGG | - | chr1.3:37590679-37590698 | MS.gene84932:CDS | 50.0% | |
| GCAGAAATGTGGGTGGGAAT+GGG | - | chr1.3:37590443-37590462 | MS.gene84932:CDS | 50.0% | |
| GGAATCACCGTGTCACGAAA+AGG | - | chr1.3:37590410-37590429 | MS.gene84932:CDS | 50.0% | |
| TGACACGGTGATTCCCAACA+AGG | + | chr1.3:37590405-37590424 | None:intergenic | 50.0% | |
| TGCAGAAATGTGGGTGGGAA+TGG | - | chr1.3:37590442-37590461 | MS.gene84932:CDS | 50.0% | |
| ! | GGCCACAAAGCATTCCATAG+AGG | - | chr1.3:37590052-37590071 | MS.gene84932:CDS | 50.0% |
| ! | TTCACCACTGGTAACGCCCT+CGG | - | chr1.3:37590028-37590047 | MS.gene84932:CDS | 55.0% |
| GAATGCTTTGTGGCCGCCGA+GGG | + | chr1.3:37590047-37590066 | None:intergenic | 60.0% | |
| GGAATGCTTTGTGGCCGCCG+AGG | + | chr1.3:37590048-37590067 | None:intergenic | 65.0% | |
| ! | ACCACTGGTAACGCCCTCGG+CGG | - | chr1.3:37590031-37590050 | MS.gene84932:CDS | 65.0% |
| !! | GCCGCCGAGGGCGTTACCAG+TGG | + | chr1.3:37590035-37590054 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 37589914 | 37590852 | 37589914 | ID=MS.gene84932 |
| chr1.3 | mRNA | 37589914 | 37590852 | 37589914 | ID=MS.gene84932.t1;Parent=MS.gene84932 |
| chr1.3 | exon | 37589914 | 37590852 | 37589914 | ID=MS.gene84932.t1.exon1;Parent=MS.gene84932.t1 |
| chr1.3 | CDS | 37589914 | 37590852 | 37589914 | ID=cds.MS.gene84932.t1;Parent=MS.gene84932.t1 |
| Gene Sequence |
| Protein sequence |
