Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84935.t1 | RHN79225.1 | 73.5 | 294 | 46 | 4 | 19 | 310 | 23 | 286 | 9.40E-78 | 300.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84935.t1 | A0A396JSM8 | 73.5 | 294 | 46 | 4 | 19 | 310 | 23 | 286 | 6.8e-78 | 300.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene84935.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84935.t1 | MTR_1g053530 | 54.286 | 245 | 83 | 8 | 16 | 255 | 16 | 236 | 2.92e-68 | 213 |
| MS.gene84935.t1 | MTR_1g046810 | 30.982 | 326 | 136 | 11 | 25 | 308 | 37 | 315 | 1.00e-29 | 115 |
| MS.gene84935.t1 | MTR_1g052185 | 30.696 | 316 | 158 | 15 | 4 | 306 | 11 | 278 | 4.66e-21 | 91.3 |
| MS.gene84935.t1 | MTR_4g073310 | 26.557 | 305 | 135 | 9 | 4 | 307 | 11 | 227 | 1.98e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 58 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATCTGTCAATGCTCTAAA+TGG | 0.239919 | 1.3:-37565367 | MS.gene84935:CDS |
| ATAATTCCAGCTCCGTTTCT+TGG | 0.269877 | 1.3:+37565231 | None:intergenic |
| GAAGAAAGTTGTTGCCTCTA+TGG | 0.295075 | 1.3:+37565473 | None:intergenic |
| TACCCTTCTTCAAAGCATAT+TGG | 0.334682 | 1.3:+37565326 | None:intergenic |
| AGAGGCAACAACTTTCTTCT+AGG | 0.336861 | 1.3:-37565469 | MS.gene84935:CDS |
| TCATCTTCACCAGGGTTGTT+TGG | 0.341135 | 1.3:+37564818 | None:intergenic |
| TGAGGAACCAACTCATGAAT+TGG | 0.350528 | 1.3:-37564786 | MS.gene84935:CDS |
| GTTCTCTTCATTATCATCAT+TGG | 0.362989 | 1.3:+37564916 | None:intergenic |
| TGCAGAAATGTGGGTGGGAT+TGG | 0.366750 | 1.3:-37565103 | MS.gene84935:CDS |
| TCATCTTCACCAGTGTTGTT+TGG | 0.374435 | 1.3:+37564857 | None:intergenic |
| ATCATTGGAAGCAGATTGAT+TGG | 0.381296 | 1.3:+37564931 | None:intergenic |
| GAGGAACCAACTCATGAATT+GGG | 0.382420 | 1.3:-37564785 | MS.gene84935:CDS |
| AAATACTTTCTCAAATCAAT+TGG | 0.400225 | 1.3:+37565274 | None:intergenic |
| CGATCGCCTAACAACCTTGT+TGG | 0.413787 | 1.3:-37565157 | MS.gene84935:CDS |
| GCAGAAATGTGGGTGGGATT+GGG | 0.432815 | 1.3:-37565102 | MS.gene84935:CDS |
| TGCCTCTATGGAATGCTTTG+TGG | 0.438332 | 1.3:+37565485 | None:intergenic |
| AATGAAGAGAACAACAATAC+TGG | 0.444824 | 1.3:-37564905 | MS.gene84935:CDS |
| TGATTTCCAACAAGGTTGTT+AGG | 0.451178 | 1.3:+37565151 | None:intergenic |
| TCATGTTGCTGCAGCAATGC+TGG | 0.457233 | 1.3:-37565191 | MS.gene84935:CDS |
| TTCTTGGTCAGATACCTCAT+TGG | 0.458462 | 1.3:+37565247 | None:intergenic |
| CAATTGGTGGAAATTGATCT+AGG | 0.480999 | 1.3:+37565290 | None:intergenic |
| TACTTTCTCAAATCAATTGG+TGG | 0.486384 | 1.3:+37565277 | None:intergenic |
| GAATGCTTTGTGGCCGCCGA+GGG | 0.493083 | 1.3:+37565495 | None:intergenic |
| TTTGCTTCATCATCTTCACC+AGG | 0.497903 | 1.3:+37564809 | None:intergenic |
| CAGCCAATATGCTTTGAAGA+AGG | 0.501688 | 1.3:-37565329 | MS.gene84935:CDS |
| TTTAACCCCAATTCATGAGT+TGG | 0.505846 | 1.3:+37564779 | None:intergenic |
| TTGGTCAGATACCTCATTGG+TGG | 0.506716 | 1.3:+37565250 | None:intergenic |
| GAAATCACCGTATCACGAAA+AGG | 0.523436 | 1.3:-37565135 | MS.gene84935:CDS |
| GATCAAACTTTGCAGAAATG+TGG | 0.524856 | 1.3:-37565113 | MS.gene84935:CDS |
| ATCTGACCAAGAAACGGAGC+TGG | 0.545214 | 1.3:-37565237 | MS.gene84935:CDS |
| TTCACCACTGGTAACGCCCT+CGG | 0.552306 | 1.3:-37565511 | MS.gene84935:CDS |
| ATCAAACTTTGCAGAAATGT+GGG | 0.554714 | 1.3:-37565112 | MS.gene84935:CDS |
| AACTTTGCAGAAATGTGGGT+GGG | 0.561670 | 1.3:-37565108 | MS.gene84935:CDS |
| AAACAATGAAAAGAACAAGT+TGG | 0.564169 | 1.3:-37565005 | MS.gene84935:CDS |
| TGAGGTATCTGACCAAGAAA+CGG | 0.565707 | 1.3:-37565243 | MS.gene84935:CDS |
| TCATCTGCATCATCATCCAC+TGG | 0.575486 | 1.3:+37564725 | None:intergenic |
| AGCCAATATGCTTTGAAGAA+GGG | 0.583138 | 1.3:-37565328 | MS.gene84935:CDS |
| GGAATGCTTTGTGGCCGCCG+AGG | 0.588007 | 1.3:+37565494 | None:intergenic |
| GCAACGAACACATGAATGGT+TGG | 0.590907 | 1.3:+37565402 | None:intergenic |
| TGAAGATGATGAAGCAAATG+AGG | 0.593094 | 1.3:-37564804 | MS.gene84935:CDS |
| AAAAGAACAAGTTGGTGGTG+AGG | 0.597114 | 1.3:-37564997 | MS.gene84935:CDS |
| AAGATGAAGATCAGAGAGCA+AGG | 0.598147 | 1.3:-37565079 | MS.gene84935:CDS |
| TTGCTTCATCATCTTCACCA+GGG | 0.604583 | 1.3:+37564810 | None:intergenic |
| GCCGCCGAGGGCGTTACCAG+TGG | 0.610498 | 1.3:+37565507 | None:intergenic |
| TGTGACAGAGAATTCACCAC+TGG | 0.614086 | 1.3:-37565523 | MS.gene84935:CDS |
| CAATGAAAAGAACAAGTTGG+TGG | 0.617009 | 1.3:-37565002 | MS.gene84935:CDS |
| AGGAACCAACTCATGAATTG+GGG | 0.629510 | 1.3:-37564784 | MS.gene84935:CDS |
| TGATACGGTGATTTCCAACA+AGG | 0.635089 | 1.3:+37565143 | None:intergenic |
| AAACTTTGCAGAAATGTGGG+TGG | 0.635743 | 1.3:-37565109 | MS.gene84935:CDS |
| GAAAGTATTTGCCACCAATG+AGG | 0.638748 | 1.3:-37565261 | MS.gene84935:CDS |
| CGATTTGAATGAGCTGCCAG+TGG | 0.642241 | 1.3:-37564741 | MS.gene84935:CDS |
| GGCCACAAAGCATTCCATAG+AGG | 0.645608 | 1.3:-37565487 | MS.gene84935:CDS |
| TGCTGCAACGAACACATGAA+TGG | 0.655011 | 1.3:+37565398 | None:intergenic |
| TGAAGATGATGAAGCAGATG+AGG | 0.659995 | 1.3:-37564843 | MS.gene84935:CDS |
| TGAAGATGATGAAGCAGATG+AGG | 0.659995 | 1.3:-37564882 | MS.gene84935:CDS |
| GATGAGGAACCAAACAACAC+TGG | 0.670709 | 1.3:-37564866 | MS.gene84935:CDS |
| ACCACTGGTAACGCCCTCGG+CGG | 0.680554 | 1.3:-37565508 | MS.gene84935:CDS |
| GATGAGGAACCAAACAACCC+TGG | 0.684317 | 1.3:-37564827 | MS.gene84935:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATACTTTCTCAAATCAAT+TGG | + | chr1.3:37565063-37565082 | None:intergenic | 20.0% |
| ! | AAACAATGAAAAGAACAAGT+TGG | - | chr1.3:37565329-37565348 | MS.gene84935:CDS | 25.0% |
| AACAAAGAAAAACATGCTGA+TGG | - | chr1.3:37564895-37564914 | MS.gene84935:CDS | 30.0% | |
| AATGAAGAGAACAACAATAC+TGG | - | chr1.3:37565429-37565448 | MS.gene84935:CDS | 30.0% | |
| ATCAAACTTTGCAGAAATGT+GGG | - | chr1.3:37565222-37565241 | MS.gene84935:CDS | 30.0% | |
| GTTCTCTTCATTATCATCAT+TGG | + | chr1.3:37565421-37565440 | None:intergenic | 30.0% | |
| TACTTTCTCAAATCAATTGG+TGG | + | chr1.3:37565060-37565079 | None:intergenic | 30.0% | |
| ! | CTTGTGATTTTTGAATGACA+AGG | + | chr1.3:37565380-37565399 | None:intergenic | 30.0% |
| ! | TTTTGAATGACAAGGCTATT+CGG | + | chr1.3:37565372-37565391 | None:intergenic | 30.0% |
| !! | AAAAAGAGTGTTGTTGATCA+TGG | - | chr1.3:37564763-37564782 | MS.gene84935:CDS | 30.0% |
| !!! | TGATCAACAACACTCTTTTT+TGG | + | chr1.3:37564763-37564782 | None:intergenic | 30.0% |
| ATCATTGGAAGCAGATTGAT+TGG | + | chr1.3:37565406-37565425 | None:intergenic | 35.0% | |
| CAATTGGTGGAAATTGATCT+AGG | + | chr1.3:37565047-37565066 | None:intergenic | 35.0% | |
| GATCAAACTTTGCAGAAATG+TGG | - | chr1.3:37565221-37565240 | MS.gene84935:CDS | 35.0% | |
| TACCCTTCTTCAAAGCATAT+TGG | + | chr1.3:37565011-37565030 | None:intergenic | 35.0% | |
| TGAAGATGATGAAGCAAATG+AGG | - | chr1.3:37565530-37565549 | MS.gene84935:CDS | 35.0% | |
| TGATTTCCAACAAGGTTGTT+AGG | + | chr1.3:37565186-37565205 | None:intergenic | 35.0% | |
| TTTAACCCCAATTCATGAGT+TGG | + | chr1.3:37565558-37565577 | None:intergenic | 35.0% | |
| ! | CAATGAAAAGAACAAGTTGG+TGG | - | chr1.3:37565332-37565351 | MS.gene84935:CDS | 35.0% |
| ! | GTTTGATCCTTTTCGTGATA+CGG | + | chr1.3:37565209-37565228 | None:intergenic | 35.0% |
| ! | TCATCTGTCAATGCTCTAAA+TGG | - | chr1.3:37564967-37564986 | MS.gene84935:CDS | 35.0% |
| !! | AGCCAATATGCTTTGAAGAA+GGG | - | chr1.3:37565006-37565025 | MS.gene84935:CDS | 35.0% |
| !!! | CAACACTCTTTTTTGGTTTG+TGG | + | chr1.3:37564756-37564775 | None:intergenic | 35.0% |
| AAACTTTGCAGAAATGTGGG+TGG | - | chr1.3:37565225-37565244 | MS.gene84935:CDS | 40.0% | |
| AACTTTGCAGAAATGTGGGT+GGG | - | chr1.3:37565226-37565245 | MS.gene84935:CDS | 40.0% | |
| AAGATGAAGATCAGAGAGCA+AGG | - | chr1.3:37565255-37565274 | MS.gene84935:CDS | 40.0% | |
| AGAGGCAACAACTTTCTTCT+AGG | - | chr1.3:37564865-37564884 | MS.gene84935:CDS | 40.0% | |
| AGGAACCAACTCATGAATTG+GGG | - | chr1.3:37565550-37565569 | MS.gene84935:CDS | 40.0% | |
| ATAATTCCAGCTCCGTTTCT+TGG | + | chr1.3:37565106-37565125 | None:intergenic | 40.0% | |
| GAAATCACCGTATCACGAAA+AGG | - | chr1.3:37565199-37565218 | MS.gene84935:CDS | 40.0% | |
| GAAGAAAGTTGTTGCCTCTA+TGG | + | chr1.3:37564864-37564883 | None:intergenic | 40.0% | |
| GAGGAACCAACTCATGAATT+GGG | - | chr1.3:37565549-37565568 | MS.gene84935:CDS | 40.0% | |
| TGAAGATGATGAAGCAGATG+AGG | - | chr1.3:37565452-37565471 | MS.gene84935:CDS | 40.0% | |
| TGAGGAACCAACTCATGAAT+TGG | - | chr1.3:37565548-37565567 | MS.gene84935:CDS | 40.0% | |
| TGATACGGTGATTTCCAACA+AGG | + | chr1.3:37565194-37565213 | None:intergenic | 40.0% | |
| TTCTTGGTCAGATACCTCAT+TGG | + | chr1.3:37565090-37565109 | None:intergenic | 40.0% | |
| TTGCTTCATCATCTTCACCA+GGG | + | chr1.3:37565527-37565546 | None:intergenic | 40.0% | |
| TTTGCTTCATCATCTTCACC+AGG | + | chr1.3:37565528-37565547 | None:intergenic | 40.0% | |
| ! | TCATCTTCACCAGTGTTGTT+TGG | + | chr1.3:37565480-37565499 | None:intergenic | 40.0% |
| ! | TGAGGTATCTGACCAAGAAA+CGG | - | chr1.3:37565091-37565110 | MS.gene84935:CDS | 40.0% |
| !! | AAAAGAACAAGTTGGTGGTG+AGG | - | chr1.3:37565337-37565356 | MS.gene84935:CDS | 40.0% |
| !! | CAGCCAATATGCTTTGAAGA+AGG | - | chr1.3:37565005-37565024 | MS.gene84935:CDS | 40.0% |
| !! | GAAAGTATTTGCCACCAATG+AGG | - | chr1.3:37565073-37565092 | MS.gene84935:CDS | 40.0% |
| GATGAGGAACCAAACAACAC+TGG | - | chr1.3:37565468-37565487 | MS.gene84935:CDS | 45.0% | |
| GCAACGAACACATGAATGGT+TGG | + | chr1.3:37564935-37564954 | None:intergenic | 45.0% | |
| TCATCTGCATCATCATCCAC+TGG | + | chr1.3:37565612-37565631 | None:intergenic | 45.0% | |
| TCATCTTCACCAGGGTTGTT+TGG | + | chr1.3:37565519-37565538 | None:intergenic | 45.0% | |
| TGCCTCTATGGAATGCTTTG+TGG | + | chr1.3:37564852-37564871 | None:intergenic | 45.0% | |
| TGCTGCAACGAACACATGAA+TGG | + | chr1.3:37564939-37564958 | None:intergenic | 45.0% | |
| TGTGACAGAGAATTCACCAC+TGG | - | chr1.3:37564811-37564830 | MS.gene84935:CDS | 45.0% | |
| TTGGTCAGATACCTCATTGG+TGG | + | chr1.3:37565087-37565106 | None:intergenic | 45.0% | |
| ATCTGACCAAGAAACGGAGC+TGG | - | chr1.3:37565097-37565116 | MS.gene84935:CDS | 50.0% | |
| CGATCGCCTAACAACCTTGT+TGG | - | chr1.3:37565177-37565196 | MS.gene84935:CDS | 50.0% | |
| CGATTTGAATGAGCTGCCAG+TGG | - | chr1.3:37565593-37565612 | MS.gene84935:CDS | 50.0% | |
| GATGAGGAACCAAACAACCC+TGG | - | chr1.3:37565507-37565526 | MS.gene84935:CDS | 50.0% | |
| GCAGAAATGTGGGTGGGATT+GGG | - | chr1.3:37565232-37565251 | MS.gene84935:CDS | 50.0% | |
| TCATGTTGCTGCAGCAATGC+TGG | - | chr1.3:37565143-37565162 | MS.gene84935:CDS | 50.0% | |
| TGCAGAAATGTGGGTGGGAT+TGG | - | chr1.3:37565231-37565250 | MS.gene84935:CDS | 50.0% | |
| ! | GGCCACAAAGCATTCCATAG+AGG | - | chr1.3:37564847-37564866 | MS.gene84935:CDS | 50.0% |
| ! | TTCACCACTGGTAACGCCCT+CGG | - | chr1.3:37564823-37564842 | MS.gene84935:CDS | 55.0% |
| GAATGCTTTGTGGCCGCCGA+GGG | + | chr1.3:37564842-37564861 | None:intergenic | 60.0% | |
| GGAATGCTTTGTGGCCGCCG+AGG | + | chr1.3:37564843-37564862 | None:intergenic | 65.0% | |
| ! | ACCACTGGTAACGCCCTCGG+CGG | - | chr1.3:37564826-37564845 | MS.gene84935:CDS | 65.0% |
| !! | GCCGCCGAGGGCGTTACCAG+TGG | + | chr1.3:37564830-37564849 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 37564712 | 37565644 | 37564712 | ID=MS.gene84935 |
| chr1.3 | mRNA | 37564712 | 37565644 | 37564712 | ID=MS.gene84935.t1;Parent=MS.gene84935 |
| chr1.3 | exon | 37564712 | 37565644 | 37564712 | ID=MS.gene84935.t1.exon1;Parent=MS.gene84935.t1 |
| chr1.3 | CDS | 37564712 | 37565644 | 37564712 | ID=cds.MS.gene84935.t1;Parent=MS.gene84935.t1 |
| Gene Sequence |
| Protein sequence |
