AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85118

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85118.t1 MTR_6g081160 40.071 282 90 6 200 412 1 272 2.12e-53 179
MS.gene85118.t1 MTR_6g081160 66.667 60 16 1 20 75 1 60 1.97e-16 79.3
MS.gene85118.t1 MTR_6g081160 60.938 64 20 2 114 172 1 64 2.57e-14 73.2
MS.gene85118.t1 MTR_5g098470 31.429 175 80 5 216 370 55 209 1.38e-13 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85118.t1 AT5G56200 38.542 96 45 2 225 320 79 160 1.14e-12 69.7
MS.gene85118.t1 AT2G26940 37.143 105 61 1 225 324 49 153 7.98e-12 65.9

Find 74 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATCTCTCTTCTGCCATTT+TGG 0.136557 2.4:+3075397 None:intergenic
CAATACAAGGTAGTTATAAT+AGG 0.224337 2.4:-3076158 MS.gene85118:CDS
CAATACAAGGTAGTAATAAT+AGG 0.229853 2.4:-3075618 MS.gene85118:CDS
TGATAATACTATGGCTAGTT+TGG 0.303196 2.4:-3075890 MS.gene85118:CDS
GTGATTTATCTTTGCCAAAA+TGG 0.304193 2.4:-3075411 MS.gene85118:CDS
GTCCTTTAAAATCTCTATTC+CGG 0.308881 2.4:+3075476 None:intergenic
CATGATGAAGTTTCTGCTTA+AGG 0.329845 2.4:+3075926 None:intergenic
ACAAAAGGTTGATAATACTA+TGG 0.403285 2.4:-3075899 MS.gene85118:CDS
GTGACCATACAAAGCATTCA+TGG 0.407800 2.4:+3075510 None:intergenic
CCAACCATGAATGCTTTGTA+TGG 0.410676 2.4:-3075514 MS.gene85118:CDS
AATACAAGGTAGTAATAATA+GGG 0.421996 2.4:-3075617 MS.gene85118:CDS
TTACTACCTTGTATTGAGAA+AGG 0.427202 2.4:+3075625 None:intergenic
TACTACCTTGTATTGAGAAA+GGG 0.428907 2.4:+3075626 None:intergenic
AATACAAGGTAGTTATAATA+GGG 0.431054 2.4:-3076157 MS.gene85118:CDS
ACACATCAGTGCAATGTTTG+TGG 0.442286 2.4:-3075742 MS.gene85118:CDS
TTTAGCAATGGAAAAGCATT+GGG 0.444822 2.4:-3076252 MS.gene85118:CDS
TTTAGTAACGGAAAAGCATT+GGG 0.453684 2.4:-3075712 MS.gene85118:CDS
ATTCGGAAAAGAGGATGAAA+AGG 0.459648 2.4:-3076368 MS.gene85118:CDS
TGAGAAAACCTATGAAGATT+CGG 0.461549 2.4:-3076385 MS.gene85118:CDS
TTTAGCAACGGAAAAGCATT+GGG 0.462037 2.4:-3075970 MS.gene85118:CDS
TGTGTTGGTGAAACATCAAA+TGG 0.466991 2.4:-3075301 MS.gene85118:CDS
AGTCAGAAAAGAGGGTGAAA+AGG 0.473192 2.4:-3075828 MS.gene85118:CDS
GAATGAGTCGTATGTTATAG+AGG 0.473597 2.4:-3075200 MS.gene85118:CDS
GATGATGAAGAAGAAATTGA+TGG 0.474180 2.4:-3075577 MS.gene85118:CDS
ACACATCAGTGCAATGTTTG+TGG 0.475496 2.4:-3076282 MS.gene85118:CDS
AGAAGAGGTAGAAAGTGTAT+TGG 0.483023 2.4:-3075376 MS.gene85118:CDS
CACATCAGTGCAATGTTTGT+GGG 0.483344 2.4:-3076281 MS.gene85118:CDS
AGTCTGAAAAGAGGATAAAG+AGG 0.489348 2.4:-3076086 MS.gene85118:CDS
AGTGATAAGCAGCCAAAGAA+AGG 0.491245 2.4:-3075241 MS.gene85118:CDS
TTTGATAAAGATAGTAAGCA+TGG 0.491925 2.4:-3076129 MS.gene85118:CDS
AAGCAGCCAAAGAAAGGAAA+AGG 0.495623 2.4:-3075235 MS.gene85118:CDS
ACTTCATCATGATTCACAAA+AGG 0.507116 2.4:-3075914 MS.gene85118:CDS
ACTCAATCATCATCAACAAA+AGG 0.509294 2.4:-3075656 MS.gene85118:CDS
AACTACCTTGTATTGAGAGA+GGG 0.509993 2.4:+3076166 None:intergenic
CCTATGAAGATTCGGAAAAG+AGG 0.512041 2.4:-3076377 MS.gene85118:CDS
ACTTAATCATCGTTCACAAA+AGG 0.517591 2.4:-3076196 MS.gene85118:CDS
CATAAAGAAGATCAAGATGA+TGG 0.521149 2.4:-3075433 MS.gene85118:CDS
GCTTAAGGAAGTGAGATCTT+CGG 0.523012 2.4:+3075941 None:intergenic
CAAACCCCTCTCTCAATACA+AGG 0.526964 2.4:-3076171 MS.gene85118:CDS
TGGGAATGGCAAGAATGAGC+TGG 0.528852 2.4:-3075281 MS.gene85118:CDS
TCTTAAGAAAGTGAGATCTC+CGG 0.549197 2.4:+3075683 None:intergenic
TCTTAAGAAAGTGAGATCTC+CGG 0.549197 2.4:+3076223 None:intergenic
CCCGTGAAGAGTCTGAAAAG+AGG 0.549764 2.4:-3076095 MS.gene85118:CDS
GAAAAGCATTGGGTGGTCAC+CGG 0.556634 2.4:-3076242 MS.gene85118:CDS
GAAAAGCATTGGGTGGTCAC+CGG 0.560535 2.4:-3075702 MS.gene85118:CDS
ACTACCTTGTATTGAGAAAG+GGG 0.563148 2.4:+3075627 None:intergenic
AAAACCCCTTTCTCAATACA+AGG 0.568454 2.4:-3075631 MS.gene85118:CDS
CCATACAAAGCATTCATGGT+TGG 0.569961 2.4:+3075514 None:intergenic
CTATGAAGAGTCAGAAAAGA+GGG 0.570873 2.4:-3075836 MS.gene85118:CDS
ATCTTCTTTATGATATTCAG+TGG 0.577502 2.4:+3075444 None:intergenic
CCTATGAAGAGTCAGAAAAG+AGG 0.578028 2.4:-3075837 MS.gene85118:CDS
TCTTTATGATATTCAGTGGG+AGG 0.578179 2.4:+3075448 None:intergenic
TGGCAGAAGAGAGATAGAAG+AGG 0.582371 2.4:-3075391 MS.gene85118:CDS
AAGCGATGAAGATGATGCTA+AGG 0.582770 2.4:-3075788 MS.gene85118:CDS
GGGAATGGCAAGAATGAGCT+GGG 0.589572 2.4:-3075280 MS.gene85118:CDS
TGAAGATGATGCTAAGGATG+AGG 0.590398 2.4:-3075782 MS.gene85118:CDS
ACTACCTTGTATTGAGAGAG+GGG 0.593179 2.4:+3076167 None:intergenic
GTGTTGGTGAAACATCAAAT+GGG 0.595282 2.4:-3075300 MS.gene85118:CDS
TAACTACCTTGTATTGAGAG+AGG 0.601425 2.4:+3076165 None:intergenic
TCTTCTTTATGATATTCAGT+GGG 0.603725 2.4:+3075445 None:intergenic
TAGTGATGAAGATGATGCTA+AGG 0.613974 2.4:-3076328 MS.gene85118:CDS
AGCAATGGAAAAGCATTGGG+TGG 0.615604 2.4:-3076249 MS.gene85118:CDS
AAGTGATGAAGATGATGCTA+AGG 0.616457 2.4:-3076046 MS.gene85118:CDS
ATGGATAAAGATCATAAGCA+TGG 0.623190 2.4:-3076411 MS.gene85118:CDS
ATGGTCACATGAGGACTCAC+CGG 0.623316 2.4:-3075495 MS.gene85118:CDS
AGCAACGGAAAAGCATTGGG+TGG 0.623901 2.4:-3075967 MS.gene85118:CDS
AGATCATAAGCATGATCATG+AGG 0.630396 2.4:-3075863 MS.gene85118:CDS
GGTGAAACATCAAATGGGAA+TGG 0.636070 2.4:-3075295 MS.gene85118:CDS
TGAAGATGATGCTAAGGATG+AGG 0.644459 2.4:-3076322 MS.gene85118:CDS
AGTAACGGAAAAGCATTGGG+TGG 0.649033 2.4:-3075709 MS.gene85118:CDS
GTGTATTGGTAGTGTTGAGG+CGG 0.666307 2.4:-3075362 MS.gene85118:CDS
AAAAGTTCTTGTCATAACGA+AGG 0.694153 2.4:+3075323 None:intergenic
AAAGTGTATTGGTAGTGTTG+AGG 0.694754 2.4:-3075365 MS.gene85118:CDS
ATGCTTTGTATGGTCACATG+AGG 0.725028 2.4:-3075504 MS.gene85118:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATACAAGGTAGTAATAATA+GGG - chr2.4:3075989-3076008 MS.gene85118:CDS 20.0%
!! AATACAAGGTAGTTATAATA+GGG - chr2.4:3075449-3075468 MS.gene85118:CDS 20.0%
! ATCTTCTTTATGATATTCAG+TGG + chr2.4:3076165-3076184 None:intergenic 25.0%
! CAATACAAGGTAGTAATAAT+AGG - chr2.4:3075988-3076007 MS.gene85118:CDS 25.0%
! CAATACAAGGTAGTTATAAT+AGG - chr2.4:3075448-3075467 MS.gene85118:CDS 25.0%
! TCTTCTTTATGATATTCAGT+GGG + chr2.4:3076164-3076183 None:intergenic 25.0%
! TTTGATAAAGATAGTAAGCA+TGG - chr2.4:3075477-3075496 MS.gene85118:CDS 25.0%
!! AATAAACCTTTTCCTTTCTT+TGG + chr2.4:3076380-3076399 None:intergenic 25.0%
!! TGTGGAAAAACTTTTAGTAA+CGG - chr2.4:3075882-3075901 MS.gene85118:CDS 25.0%
!!! ACAAAAGGTTGATAATACTA+TGG - chr2.4:3075707-3075726 MS.gene85118:CDS 25.0%
AAAAGTTCTTGTCATAACGA+AGG + chr2.4:3076286-3076305 None:intergenic 30.0%
ACTCAATCATCATCAACAAA+AGG - chr2.4:3075950-3075969 MS.gene85118:CDS 30.0%
ACTTAATCATCGTTCACAAA+AGG - chr2.4:3075410-3075429 MS.gene85118:CDS 30.0%
ACTTCATCATGATTCACAAA+AGG - chr2.4:3075692-3075711 MS.gene85118:CDS 30.0%
ATGGATAAAGATCATAAGCA+TGG - chr2.4:3075195-3075214 MS.gene85118:CDS 30.0%
CATAAAGAAGATCAAGATGA+TGG - chr2.4:3076173-3076192 MS.gene85118:CDS 30.0%
GATGATGAAGAAGAAATTGA+TGG - chr2.4:3076029-3076048 MS.gene85118:CDS 30.0%
GTGATTTATCTTTGCCAAAA+TGG - chr2.4:3076195-3076214 MS.gene85118:CDS 30.0%
TACTACCTTGTATTGAGAAA+GGG + chr2.4:3075983-3076002 None:intergenic 30.0%
TGAGAAAACCTATGAAGATT+CGG - chr2.4:3075221-3075240 MS.gene85118:CDS 30.0%
TTACTACCTTGTATTGAGAA+AGG + chr2.4:3075984-3076003 None:intergenic 30.0%
! ACACATCAATGCAATTTTTG+TGG - chr2.4:3075606-3075625 MS.gene85118:CDS 30.0%
! TATGACAAGAACTTTTGTGT+TGG - chr2.4:3076290-3076309 MS.gene85118:CDS 30.0%
! TGATAATACTATGGCTAGTT+TGG - chr2.4:3075716-3075735 MS.gene85118:CDS 30.0%
! TGTGGAAAAACTTTTAGCAA+CGG - chr2.4:3075624-3075643 MS.gene85118:CDS 30.0%
!! GTCCTTTAAAATCTCTATTC+CGG + chr2.4:3076133-3076152 None:intergenic 30.0%
!! TTTAGCAATGGAAAAGCATT+GGG - chr2.4:3075354-3075373 MS.gene85118:CDS 30.0%
!! TTTAGTAACGGAAAAGCATT+GGG - chr2.4:3075894-3075913 MS.gene85118:CDS 30.0%
!! TTTTAGCAATGGAAAAGCAT+TGG - chr2.4:3075353-3075372 MS.gene85118:CDS 30.0%
!! TTTTAGTAACGGAAAAGCAT+TGG - chr2.4:3075893-3075912 MS.gene85118:CDS 30.0%
AAAACCCCTTTCTCAATACA+AGG - chr2.4:3075975-3075994 MS.gene85118:CDS 35.0%
AACTACCTTGTATTGAGAGA+GGG + chr2.4:3075443-3075462 None:intergenic 35.0%
AAGTGATGAAGATGATGCTA+AGG - chr2.4:3075560-3075579 MS.gene85118:CDS 35.0%
ACTACCTTGTATTGAGAAAG+GGG + chr2.4:3075982-3076001 None:intergenic 35.0%
AGATCATAAGCATGATCATG+AGG - chr2.4:3075743-3075762 MS.gene85118:CDS 35.0%
AGTCTGAAAAGAGGATAAAG+AGG - chr2.4:3075520-3075539 MS.gene85118:CDS 35.0%
ATTCGGAAAAGAGGATGAAA+AGG - chr2.4:3075238-3075257 MS.gene85118:CDS 35.0%
CATGATGAAGTTTCTGCTTA+AGG + chr2.4:3075683-3075702 None:intergenic 35.0%
CTATGAAGAGTCAGAAAAGA+GGG - chr2.4:3075770-3075789 MS.gene85118:CDS 35.0%
GAATGAGTCGTATGTTATAG+AGG - chr2.4:3076406-3076425 MS.gene85118:CDS 35.0%
TAACTACCTTGTATTGAGAG+AGG + chr2.4:3075444-3075463 None:intergenic 35.0%
TAGTGATGAAGATGATGCTA+AGG - chr2.4:3075278-3075297 MS.gene85118:CDS 35.0%
TCTTAAGAAAGTGAGATCTC+CGG + chr2.4:3075386-3075405 None:intergenic 35.0%
TCTTTATGATATTCAGTGGG+AGG + chr2.4:3076161-3076180 None:intergenic 35.0%
! AGAAGAGGTAGAAAGTGTAT+TGG - chr2.4:3076230-3076249 MS.gene85118:CDS 35.0%
! CACCGGAATAGAGATTTTAA+AGG - chr2.4:3076128-3076147 MS.gene85118:CDS 35.0%
! GTGTTGGTGAAACATCAAAT+GGG - chr2.4:3076306-3076325 MS.gene85118:CDS 35.0%
! TGTGGGAAAACTTTTAGCAA+TGG - chr2.4:3075342-3075361 MS.gene85118:CDS 35.0%
! TGTGTTGGTGAAACATCAAA+TGG - chr2.4:3076305-3076324 MS.gene85118:CDS 35.0%
! TTTTTGCAGAGAGAACAAGT+TGG + chr2.4:3076065-3076084 None:intergenic 35.0%
!! AAAGTGTATTGGTAGTGTTG+AGG - chr2.4:3076241-3076260 MS.gene85118:CDS 35.0%
!! TTTAGCAACGGAAAAGCATT+GGG - chr2.4:3075636-3075655 MS.gene85118:CDS 35.0%
!! TTTTAGCAACGGAAAAGCAT+TGG - chr2.4:3075635-3075654 MS.gene85118:CDS 35.0%
AAGCAGCCAAAGAAAGGAAA+AGG - chr2.4:3076371-3076390 MS.gene85118:CDS 40.0%
AAGCGATGAAGATGATGCTA+AGG - chr2.4:3075818-3075837 MS.gene85118:CDS 40.0%
ACACATCAGTGCAATGTTTG+TGG - chr2.4:3075324-3075343 MS.gene85118:CDS 40.0%
ACTACCTTGTATTGAGAGAG+GGG + chr2.4:3075442-3075461 None:intergenic 40.0%
AGTCAGAAAAGAGGGTGAAA+AGG - chr2.4:3075778-3075797 MS.gene85118:CDS 40.0%
AGTGATAAGCAGCCAAAGAA+AGG - chr2.4:3076365-3076384 MS.gene85118:CDS 40.0%
ATGCTTTGTATGGTCACATG+AGG - chr2.4:3076102-3076121 MS.gene85118:CDS 40.0%
CACATCAGTGCAATGTTTGT+GGG - chr2.4:3075325-3075344 MS.gene85118:CDS 40.0%
CCAACCATGAATGCTTTGTA+TGG - chr2.4:3076092-3076111 MS.gene85118:CDS 40.0%
CCTATGAAGAGTCAGAAAAG+AGG - chr2.4:3075769-3075788 MS.gene85118:CDS 40.0%
CCTATGAAGATTCGGAAAAG+AGG - chr2.4:3075229-3075248 MS.gene85118:CDS 40.0%
GCTTAAGGAAGTGAGATCTT+CGG + chr2.4:3075668-3075687 None:intergenic 40.0%
GGTGAAACATCAAATGGGAA+TGG - chr2.4:3076311-3076330 MS.gene85118:CDS 40.0%
TGAAGATGATGCTAAGGATG+AGG - chr2.4:3075284-3075303 MS.gene85118:CDS 40.0%
! CCATACAAAGCATTCATGGT+TGG + chr2.4:3076095-3076114 None:intergenic 40.0%
! CCTCTTTTCCGAATCTTCAT+AGG + chr2.4:3075232-3075251 None:intergenic 40.0%
! CCTCTTTTCTGACTCTTCAT+AGG + chr2.4:3075772-3075791 None:intergenic 40.0%
! GTGACCATACAAAGCATTCA+TGG + chr2.4:3076099-3076118 None:intergenic 40.0%
! TCCTCTTTTCAGACTCTTCA+CGG + chr2.4:3075515-3075534 None:intergenic 40.0%
!! CTATCTCTCTTCTGCCATTT+TGG + chr2.4:3076212-3076231 None:intergenic 40.0%
CAAACCCCTCTCTCAATACA+AGG - chr2.4:3075435-3075454 MS.gene85118:CDS 45.0%
TGGCAGAAGAGAGATAGAAG+AGG - chr2.4:3076215-3076234 MS.gene85118:CDS 45.0%
! CCTCTTTTCAGACTCTTCAC+GGG + chr2.4:3075514-3075533 None:intergenic 45.0%
!! AGCAATGGAAAAGCATTGGG+TGG - chr2.4:3075357-3075376 MS.gene85118:CDS 45.0%
!! AGTAACGGAAAAGCATTGGG+TGG - chr2.4:3075897-3075916 MS.gene85118:CDS 45.0%
!! GTGTATTGGTAGTGTTGAGG+CGG - chr2.4:3076244-3076263 MS.gene85118:CDS 45.0%
ATGGTCACATGAGGACTCAC+CGG - chr2.4:3076111-3076130 MS.gene85118:CDS 50.0%
CCCGTGAAGAGTCTGAAAAG+AGG - chr2.4:3075511-3075530 MS.gene85118:CDS 50.0%
GGGAATGGCAAGAATGAGCT+GGG - chr2.4:3076326-3076345 MS.gene85118:CDS 50.0%
TGGGAATGGCAAGAATGAGC+TGG - chr2.4:3076325-3076344 MS.gene85118:CDS 50.0%
!! AGCAACGGAAAAGCATTGGG+TGG - chr2.4:3075639-3075658 MS.gene85118:CDS 50.0%
!! GAAAAGCATTGGGTGGTCAC+CGG - chr2.4:3075364-3075383 MS.gene85118:CDS 50.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 3075195 3076433 3075195 ID=MS.gene85118
chr2.4 mRNA 3075195 3076433 3075195 ID=MS.gene85118.t1;Parent=MS.gene85118
chr2.4 exon 3075195 3076433 3075195 ID=MS.gene85118.t1.exon1;Parent=MS.gene85118.t1
chr2.4 CDS 3075195 3076433 3075195 ID=cds.MS.gene85118.t1;Parent=MS.gene85118.t1
Gene Sequence

>MS.gene85118

ATGGATAAAGATCATAAGCATGGTTATGAGAAAACCTATGAAGATTCGGAAAAGAGGATGAAAAGGTCTTCATGTTCACTATCTAGTGATGAAGATGATGCTAAGGATGAGGTTTCTGTTCAAGAATCTACACATCAGTGCAATGTTTGTGGGAAAACTTTTAGCAATGGAAAAGCATTGGGTGGTCACCGGAGATCTCACTTTCTTAAGAAGAAACTTAATCATCGTTCACAAAAGGTACAAACCCCTCTCTCAATACAAGGTAGTTATAATAGGGCTAGTTTTGATAAAGATAGTAAGCATGGTTTTGAGAACACCCGTGAAGAGTCTGAAAAGAGGATAAAGAGGTCTTTTAGTTCTCTATCAAGTGATGAAGATGATGCTAAGGATGAGCTTTCTATTCAAGTATCTACACATCAATGCAATTTTTGTGGAAAAACTTTTAGCAACGGAAAAGCATTGGGTGGTCACCGAAGATCTCACTTCCTTAAGCAGAAACTTCATCATGATTCACAAAAGGTTGATAATACTATGGCTAGTTTGGATAAAGATCATAAGCATGATCATGAGGATACCTATGAAGAGTCAGAAAAGAGGGTGAAAAGGTCAGCTTGTTCTCTATCAAGCGATGAAGATGATGCTAAGGATGAGGTTTCTGTTCAAGAATCTACACATCAGTGCAATGTTTGTGGAAAAACTTTTAGTAACGGAAAAGCATTGGGTGGTCACCGGAGATCTCACTTTCTTAAGAAGAAACTCAATCATCATCAACAAAAGGTGAAAACCCCTTTCTCAATACAAGGTAGTAATAATAGGGCTAGTTTTGATGATTATGATGATGAAGAAGAAATTGATGGCATAGAGAAACCAACTTGTTCTCTCTGCAAAAAGAAGTTTCCAACCATGAATGCTTTGTATGGTCACATGAGGACTCACCGGAATAGAGATTTTAAAGGACTTAATCCTCCCACTGAATATCATAAAGAAGATCAAGATGATGGTGATTTATCTTTGCCAAAATGGCAGAAGAGAGATAGAAGAGGTAGAAAGTGTATTGGTAGTGTTGAGGCGGCTGCAAATCTTTTGCACCTTCGTTATGACAAGAACTTTTGTGTTGGTGAAACATCAAATGGGAATGGCAAGAATGAGCTGGGCAGTGATAGAAACAGCAGTGATAAGCAGCCAAAGAAAGGAAAAGGTTTATTTGATTTGAATGAGTCGTATGTTATAGAGGACTAA
Protein sequence

>MS.gene85118.t1

MDKDHKHGYEKTYEDSEKRMKRSSCSLSSDEDDAKDEVSVQESTHQCNVCGKTFSNGKALGGHRRSHFLKKKLNHRSQKVQTPLSIQGSYNRASFDKDSKHGFENTREESEKRIKRSFSSLSSDEDDAKDELSIQVSTHQCNFCGKTFSNGKALGGHRRSHFLKQKLHHDSQKVDNTMASLDKDHKHDHEDTYEESEKRVKRSACSLSSDEDDAKDEVSVQESTHQCNVCGKTFSNGKALGGHRRSHFLKKKLNHHQQKVKTPFSIQGSNNRASFDDYDDEEEIDGIEKPTCSLCKKKFPTMNALYGHMRTHRNRDFKGLNPPTEYHKEDQDDGDLSLPKWQKRDRRGRKCIGSVEAAANLLHLRYDKNFCVGETSNGNGKNELGSDRNSSDKQPKKGKGLFDLNESYVIED