AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85121

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85121.t1 MTR_2g101720 97.733 794 14 1 1 790 1 794 0.0 1612
MS.gene85121.t1 MTR_8g432540 43.321 801 374 15 34 788 43 809 0.0 640
MS.gene85121.t1 MTR_2g088470 44.242 712 354 10 106 788 38 735 0.0 622
MS.gene85121.t1 MTR_4g047800 42.800 750 377 13 66 788 8 732 0.0 616
MS.gene85121.t1 MTR_7g076080 41.466 791 391 14 39 788 18 777 0.0 614
MS.gene85121.t1 MTR_1g101280 42.450 702 350 13 133 785 54 750 0.0 574
MS.gene85121.t1 MTR_6g477820 40.393 713 353 13 133 787 27 725 0.0 560
MS.gene85121.t1 MTR_5g029320 37.260 781 402 16 82 785 9 778 2.43e-167 503
MS.gene85121.t1 MTR_0198s0030 38.615 751 376 13 95 785 26 751 4.75e-167 501
MS.gene85121.t1 MTR_5g005600 38.218 696 379 14 126 785 36 716 1.76e-154 468
MS.gene85121.t1 MTR_7g427710 37.518 685 363 16 122 787 2 640 2.18e-150 454
MS.gene85121.t1 MTR_2g030570 32.425 697 434 13 118 785 7 695 3.45e-117 370
MS.gene85121.t1 MTR_2g030600 31.313 693 440 14 118 785 7 688 3.18e-107 343
MS.gene85121.t1 MTR_2g030660 31.268 678 414 19 125 785 5 647 1.16e-93 306
MS.gene85121.t1 MTR_7g427690 36.461 373 211 7 299 663 57 411 1.07e-82 271
MS.gene85121.t1 MTR_2g083350 30.784 536 321 13 290 785 6 531 9.58e-75 253
MS.gene85121.t1 MTR_2g083330 29.942 521 320 10 301 786 16 526 2.91e-68 235
MS.gene85121.t1 MTR_7g427700 35.517 290 155 5 134 416 15 279 5.87e-50 182
MS.gene85121.t1 MTR_7g427700 37.888 161 82 5 637 787 281 433 2.71e-22 100
MS.gene85121.t1 MTR_0653s0020 35.106 188 103 5 482 667 3 173 2.07e-35 133
MS.gene85121.t1 MTR_2g101740 75.281 89 7 1 702 790 1 74 9.77e-35 127
MS.gene85121.t1 MTR_2g030690 31.741 293 174 10 503 785 1 277 1.54e-29 119
MS.gene85121.t1 MTR_7g027265 44.030 134 73 1 123 256 5 136 1.87e-29 114
MS.gene85121.t1 MTR_5g046040 38.922 167 92 4 133 296 31 190 1.27e-26 112
MS.gene85121.t1 MTR_2g094520 24.615 520 309 18 138 636 23 480 1.21e-23 107
MS.gene85121.t1 MTR_3g109800 21.350 726 409 25 138 779 20 667 5.75e-23 105
MS.gene85121.t1 MTR_3g109800 21.350 726 409 25 138 779 20 667 5.81e-23 105
MS.gene85121.t1 MTR_3g109800 21.245 739 420 25 125 779 7 667 8.34e-23 104
MS.gene85121.t1 MTR_4g058970 23.119 545 316 18 122 636 12 483 7.93e-22 101
MS.gene85121.t1 MTR_8g013980 22.159 528 315 17 138 636 15 475 5.64e-21 99.0
MS.gene85121.t1 MTR_2g101190 21.852 540 328 19 124 636 5 477 6.91e-21 98.6
MS.gene85121.t1 MTR_7g027605 43.956 91 50 1 387 477 2 91 4.83e-20 85.9
MS.gene85121.t1 MTR_0653s0010 41.935 124 55 4 673 787 1 116 1.30e-18 82.8
MS.gene85121.t1 MTR_2g101750 80.435 46 9 0 745 790 9 54 7.13e-18 78.6
MS.gene85121.t1 MTR_6g027290 29.032 155 102 3 123 273 12 162 3.03e-16 77.8
MS.gene85121.t1 MTR_2g451350 26.667 135 99 0 311 445 1 135 3.07e-14 70.9
MS.gene85121.t1 MTR_7g103340 41.176 102 55 1 121 222 51 147 1.09e-13 71.6
MS.gene85121.t1 MTR_1g105420 31.765 170 92 6 194 343 5 170 2.04e-13 70.5
MS.gene85121.t1 MTR_2g030700 28.986 138 87 3 123 260 6 132 1.10e-12 67.0
MS.gene85121.t1 MTR_2g101730 54.545 66 17 2 725 790 19 71 1.34e-12 63.9
MS.gene85121.t1 MTR_2g030680 28.986 138 87 3 123 260 6 132 7.53e-12 64.7
MS.gene85121.t1 MTR_1g054285 36.842 114 68 1 107 220 127 236 9.69e-12 67.0
MS.gene85121.t1 MTR_4g100550 34.459 148 82 4 73 220 70 202 4.88e-11 64.7
MS.gene85121.t1 MTR_6g011610 30.457 197 105 5 26 222 16 180 6.28e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85121.t1 AT1G79840 62.403 774 258 10 21 789 1 746 0.0 961
MS.gene85121.t1 AT1G79840 62.403 774 258 10 21 789 30 775 0.0 957
MS.gene85121.t1 AT1G79840 64.580 703 229 7 92 789 1 688 0.0 924
MS.gene85121.t1 AT1G79840 64.580 703 229 7 92 789 1 688 0.0 924
MS.gene85121.t1 AT1G79840 62.404 649 211 10 21 664 1 621 0.0 798
MS.gene85121.t1 AT4G04890 43.175 718 365 10 106 790 33 740 0.0 619
MS.gene85121.t1 AT4G04890 43.175 718 365 10 106 790 33 740 0.0 619
MS.gene85121.t1 AT1G79840 60.377 530 177 10 21 545 1 502 0.0 612
MS.gene85121.t1 AT4G00730 44.302 702 351 11 111 788 116 801 0.0 609
MS.gene85121.t1 AT4G21750 43.058 713 347 9 128 788 55 760 0.0 608
MS.gene85121.t1 AT4G21750 43.058 713 347 9 128 788 55 760 0.0 608
MS.gene85121.t1 AT4G21750 43.058 713 347 9 128 788 55 760 0.0 608
MS.gene85121.t1 AT4G21750 43.058 713 347 9 128 788 55 760 0.0 608
MS.gene85121.t1 AT1G05230 42.999 707 355 9 106 788 35 717 0.0 603
MS.gene85121.t1 AT1G05230 42.877 709 355 9 106 788 35 719 0.0 602
MS.gene85121.t1 AT1G05230 42.877 709 355 9 106 788 35 719 0.0 602
MS.gene85121.t1 AT1G05230 42.877 709 355 9 106 788 35 719 0.0 602
MS.gene85121.t1 AT1G05230 42.877 709 355 9 106 788 35 719 0.0 602
MS.gene85121.t1 AT1G05230 42.877 709 355 9 106 788 35 719 0.0 602
MS.gene85121.t1 AT1G05230 42.877 709 355 9 106 788 35 719 0.0 602
MS.gene85121.t1 AT1G05230 42.877 709 355 9 106 788 35 719 0.0 602
MS.gene85121.t1 AT1G05230 42.877 709 355 9 106 788 35 719 0.0 602
MS.gene85121.t1 AT1G05230 42.877 709 355 9 106 788 35 719 0.0 602
MS.gene85121.t1 AT1G05230 42.877 709 355 9 106 788 35 719 0.0 602
MS.gene85121.t1 AT3G61150 42.412 738 342 12 114 788 89 806 0.0 593
MS.gene85121.t1 AT1G05230 42.981 577 298 6 106 663 35 599 3.62e-166 494
MS.gene85121.t1 AT5G52170 40.955 691 359 14 106 785 26 678 4.75e-166 496
MS.gene85121.t1 AT5G52170 40.955 691 359 14 106 785 26 678 4.75e-166 496
MS.gene85121.t1 AT5G52170 40.666 691 366 12 106 785 26 683 1.88e-164 492
MS.gene85121.t1 AT1G17920 38.796 714 372 15 107 787 2 683 1.35e-163 490
MS.gene85121.t1 AT1G17920 38.796 714 372 15 107 787 2 683 1.35e-163 490
MS.gene85121.t1 AT1G73360 39.571 700 360 18 134 786 31 714 1.72e-163 491
MS.gene85121.t1 AT2G32370 39.856 695 366 14 121 788 54 723 1.12e-159 481
MS.gene85121.t1 AT5G46880 36.043 738 386 18 110 785 93 806 1.21e-146 451
MS.gene85121.t1 AT5G46880 36.043 738 386 18 110 785 93 806 1.21e-146 451
MS.gene85121.t1 AT5G46880 36.043 738 386 18 110 785 93 806 1.21e-146 451
MS.gene85121.t1 AT4G17710 37.801 664 352 16 135 785 88 703 1.02e-133 414
MS.gene85121.t1 AT5G17320 32.650 732 415 17 116 788 6 718 8.78e-125 390
MS.gene85121.t1 AT3G03260 33.102 722 406 18 120 789 2 698 6.40e-123 385
MS.gene85121.t1 AT4G00730 42.763 456 226 7 111 544 116 558 2.27e-120 374
MS.gene85121.t1 AT4G25530 32.999 697 423 15 107 788 18 685 3.77e-119 375
MS.gene85121.t1 AT1G34650 32.575 703 401 18 142 788 23 708 2.11e-105 339
MS.gene85121.t1 AT3G03260 32.123 551 325 13 120 635 2 538 1.05e-88 290
MS.gene85121.t1 AT5G07260 20.632 538 371 15 201 733 10 496 1.54e-27 118
MS.gene85121.t1 AT5G07260 20.664 542 371 16 201 733 10 501 2.75e-27 117
MS.gene85121.t1 AT4G32880 21.023 704 413 26 125 752 2 638 2.80e-24 109
MS.gene85121.t1 AT5G60690 23.062 529 298 21 138 636 25 474 3.18e-21 99.8
MS.gene85121.t1 AT1G30490 22.409 656 383 26 126 720 9 599 7.09e-21 98.6
MS.gene85121.t1 AT2G34710 21.265 743 421 29 128 785 15 678 2.17e-18 90.9
MS.gene85121.t1 AT4G26920 27.179 195 124 6 289 478 47 228 1.30e-14 77.8
MS.gene85121.t1 AT3G60390 39.623 106 58 2 118 222 146 246 1.52e-11 67.0
MS.gene85121.t1 AT4G16780 36.752 117 62 2 110 220 101 211 5.34e-11 64.7

Find 169 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAGACTCCATTGTTAATT+TGG 0.153641 2.4:+3118084 None:intergenic
CTCCAAGGCAAGTCAAATTT+TGG 0.154977 2.4:-3122873 MS.gene85121:CDS
GTCAACAGTGGCCTAGCTTT+TGG 0.171217 2.4:-3119161 MS.gene85121:CDS
TGATTTGGGTACGACGATTT+TGG 0.178481 2.4:+3122848 None:intergenic
TTGGCTTTAAATAGGTAGTA+TGG 0.217597 2.4:-3119238 MS.gene85121:intron
TTCAAATTCATCCATTGATT+TGG 0.230510 2.4:+3124159 None:intergenic
GAGAAGCATTTGTAAGGATT+TGG 0.247426 2.4:+3118113 None:intergenic
GGGGCAACAAGCCTTATTTA+TGG 0.248716 2.4:+3122634 None:intergenic
ATTTAAATAATACCTTGATT+TGG 0.259779 2.4:+3122833 None:intergenic
TGTGGTGGGGACCCCACAAT+TGG 0.267327 2.4:+3122613 None:intergenic
AGAGAATATTAAAAGCTCTT+TGG 0.271894 2.4:-3121706 MS.gene85121:CDS
CGGCATGGCGGTGCGGCGGC+GGG 0.287501 2.4:-3124298 MS.gene85121:CDS
GTTTCCATGTTTAATATCTA+AGG 0.290461 2.4:-3121242 MS.gene85121:CDS
TTTGTGCTGTTTCTTCTATA+TGG 0.290578 2.4:-3118750 MS.gene85121:CDS
TCGGCATGGCGGTGCGGCGG+CGG 0.292792 2.4:-3124299 MS.gene85121:CDS
CATAACCTCGGCTTTGAGTT+TGG 0.297585 2.4:+3122538 None:intergenic
GTTGTCTTCTACTTTGTCTA+TGG 0.298498 2.4:+3119508 None:intergenic
GGCATGGCGGTGCGGCGGCG+GGG 0.303946 2.4:-3124297 MS.gene85121:CDS
GGGGAGGAAGGGAGTACAAT+AGG 0.308975 2.4:-3124232 MS.gene85121:CDS
GTGGTGGGGACCCCACAATT+GGG 0.315163 2.4:+3122614 None:intergenic
GAGAGGCTGAGAGCAGCTTT+AGG 0.319197 2.4:-3121780 MS.gene85121:CDS
GAAGCTTCAATGGATCTCTT+TGG 0.328107 2.4:+3121489 None:intergenic
TTTAAATAATACCTTGATTT+GGG 0.331757 2.4:+3122834 None:intergenic
CTCACCAGTTGAATCCTTCA+TGG 0.359314 2.4:+3119067 None:intergenic
TCCATTGATTTGGATTTAGT+TGG 0.361488 2.4:+3124169 None:intergenic
ATTGCCTCTGTCTTGTCCTT+TGG 0.362072 2.4:+3118519 None:intergenic
GCATGGCGGTGCGGCGGCGG+GGG 0.367383 2.4:-3124296 MS.gene85121:CDS
GGTAGTATGGGTGGAGCATT+TGG 0.373488 2.4:-3119225 MS.gene85121:CDS
GAAATAAGCAGCGAGTATTC+AGG 0.374487 2.4:-3124193 MS.gene85121:CDS
TGGAGAGGGAAAGAAGTCTT+TGG 0.377024 2.4:+3124760 None:intergenic
GAGGTGGAAGAAGGGGAGGA+AGG 0.380425 2.4:-3124244 MS.gene85121:CDS
CCTACGAGAGACCATAAATA+AGG 0.385213 2.4:-3122645 MS.gene85121:CDS
AAGAAATCAAACAAAACATT+AGG 0.391068 2.4:+3118715 None:intergenic
ATAAGAACAAGAAGAAGAAA+AGG 0.391486 2.4:-3124078 MS.gene85121:CDS
AACCAAAATTTGACTTGCCT+TGG 0.392620 2.4:+3122871 None:intergenic
CATTGCACCATCTCTGTTTG+TGG 0.393009 2.4:+3122595 None:intergenic
ACTAAGGTTACTAGTAAAAC+TGG 0.395797 2.4:-3118838 MS.gene85121:CDS
TTTCTGATGGCTTGGAATCA+AGG 0.397526 2.4:-3118204 MS.gene85121:CDS
TTCAATTGTTTCTGATGGCT+TGG 0.398938 2.4:-3118212 MS.gene85121:CDS
CAAGAATGGTGCAGTGCAAC+TGG 0.400659 2.4:-3121179 MS.gene85121:intron
AGTAATATGGAGGTGGAAGA+AGG 0.401643 2.4:-3124253 MS.gene85121:CDS
AACAAAACATTAGGAGATAT+AGG 0.411699 2.4:+3118724 None:intergenic
CAACAACTTAGCAAGCAATT+AGG 0.414776 2.4:-3122901 MS.gene85121:CDS
AGAGGCTGAGAGCAGCTTTA+GGG 0.415023 2.4:-3121779 MS.gene85121:CDS
TCTTTGGCTTTAGAAGTAGA+AGG 0.415922 2.4:+3124776 None:intergenic
ACAAAATACACTTCTCTAGT+TGG 0.422278 2.4:+3119581 None:intergenic
TTATAAGCTCCTCCATTGCT+TGG 0.425119 2.4:+3121628 None:intergenic
TGAAAATCCTGAGGGCAGTA+TGG 0.428055 2.4:+3118231 None:intergenic
AATCAGAAATAGAGAAACTA+AGG 0.429794 2.4:-3122682 MS.gene85121:CDS
AGGTGGAAGAAGGGGAGGAA+GGG 0.430407 2.4:-3124243 MS.gene85121:CDS
TGTATCTCTTGAAGCTTCAA+TGG 0.438168 2.4:+3121479 None:intergenic
TGGATCTCTTTGGTCTTCCA+TGG 0.443218 2.4:+3121499 None:intergenic
CTGTAGGAGAAGCATTTGTA+AGG 0.443396 2.4:+3118107 None:intergenic
AGGCTTGTTGCCCCAATTGT+GGG 0.447887 2.4:-3122625 MS.gene85121:CDS
TTAGGTGTTGCCACATTGGC+TGG 0.448272 2.4:-3118943 MS.gene85121:intron
ATTGAAGCTTCAAGAGATAC+AGG 0.451772 2.4:-3121477 MS.gene85121:CDS
CCCATCTGGTTGCATTATTG+AGG 0.452497 2.4:-3119453 MS.gene85121:CDS
GTAATATGGAGGTGGAAGAA+GGG 0.456514 2.4:-3124252 MS.gene85121:CDS
TACGCACCTGTGGATATTAC+CGG 0.457137 2.4:-3118295 MS.gene85121:CDS
TCTTGATGAGTCAAGAATAA+TGG 0.457181 2.4:-3121661 MS.gene85121:CDS
ACAAGTCTAAATTGTGAAGA+TGG 0.459079 2.4:-3118016 MS.gene85121:CDS
TCCAACTAAATCCAAATCAA+TGG 0.462181 2.4:-3124170 MS.gene85121:CDS
GTGCATCAAGCTTCCATACA+TGG 0.464344 2.4:-3118861 MS.gene85121:CDS
GCTTTAGGGAAATATGCATC+AGG 0.464608 2.4:-3121765 MS.gene85121:CDS
GACGTTCGCATTGAAGTTGA+AGG 0.466117 2.4:+3119117 None:intergenic
AAATGCAGAAAACGCCCATC+TGG 0.466193 2.4:-3119467 MS.gene85121:CDS
AATGATCCTAGTGAACATCT+TGG 0.466350 2.4:-3118781 MS.gene85121:CDS
TGCTATGTTTGCAGAATCAA+TGG 0.466472 2.4:-3121273 MS.gene85121:intron
TCCTTTGGCTAAATTTGCAA+TGG 0.466744 2.4:+3118534 None:intergenic
TAGTAACCTTAGTCCATGTA+TGG 0.466982 2.4:+3118848 None:intergenic
CACAAATTCTTACGAATCAA+TGG 0.474074 2.4:-3118323 MS.gene85121:CDS
GCTAAGAGAGAGAGCTGGAG+AGG 0.488674 2.4:+3124745 None:intergenic
TAATATGGAGGTGGAAGAAG+GGG 0.489966 2.4:-3124251 MS.gene85121:CDS
CTTATTTATGGTCTCTCGTA+GGG 0.492068 2.4:+3122646 None:intergenic
ACTTCTCTAGTTGGAACCAT+TGG 0.492214 2.4:+3119590 None:intergenic
GTAAAACTGGAGAAGACATA+AGG 0.495014 2.4:-3118825 MS.gene85121:CDS
ATTATTGAGGATAAGTCAAA+TGG 0.496385 2.4:-3119440 MS.gene85121:CDS
AAGGCTTGTTGCCCCAATTG+TGG 0.499744 2.4:-3122626 MS.gene85121:CDS
TGGCTTTAAATAGGTAGTAT+GGG 0.500533 2.4:-3119237 MS.gene85121:intron
CTTATAAAGATGGCAACAAT+GGG 0.505105 2.4:-3121612 MS.gene85121:CDS
CAGTGGGATATAATGTCTAA+TGG 0.509556 2.4:-3118571 MS.gene85121:CDS
GGTATTCAATCCGTGATGAC+CGG 0.513881 2.4:-3118274 MS.gene85121:CDS
CCTTATTTATGGTCTCTCGT+AGG 0.514357 2.4:+3122645 None:intergenic
GCACCATCTCTGTTTGTGGT+GGG 0.514855 2.4:+3122599 None:intergenic
AAATCAATGGATGAATTTGA+AGG 0.515118 2.4:-3124157 MS.gene85121:CDS
AATCAAAAGAAAACAATATG+TGG 0.518388 2.4:-3118363 MS.gene85121:CDS
TGCACCATCTCTGTTTGTGG+TGG 0.525119 2.4:+3122598 None:intergenic
TGGCTTGGAATCAAGGCAAA+TGG 0.525320 2.4:-3118197 MS.gene85121:CDS
TCCTCAATAATGCAACCAGA+TGG 0.528035 2.4:+3119452 None:intergenic
GTTCCCATGAAGGATTCAAC+TGG 0.531129 2.4:-3119071 MS.gene85121:intron
AAATCGTCGTACCCAAATCA+AGG 0.531425 2.4:-3122845 MS.gene85121:intron
CACCATCTCTGTTTGTGGTG+GGG 0.533160 2.4:+3122600 None:intergenic
AAGCTTCCATACATGGACTA+AGG 0.539054 2.4:-3118854 MS.gene85121:CDS
TGGTGGGGACCCCACAATTG+GGG 0.540284 2.4:+3122615 None:intergenic
GCTTATAAAGATGGCAACAA+TGG 0.540324 2.4:-3121613 MS.gene85121:CDS
GGAGAAGGTTCTAACAAGAA+TGG 0.540967 2.4:-3121193 MS.gene85121:CDS
GTTGCAAATGTTAACACCAA+TGG 0.544408 2.4:-3119606 MS.gene85121:CDS
ATAGTTAAGTATTTCACGAC+CGG 0.544434 2.4:+3121560 None:intergenic
TCCATTGTTAATTTGGCTGT+AGG 0.545967 2.4:+3118091 None:intergenic
GGCAACAAACGTTCCCATGA+AGG 0.546780 2.4:-3119081 MS.gene85121:CDS
AATGCCTTGCCCCAAAAGCT+AGG 0.549012 2.4:+3119150 None:intergenic
TCCATTGCAAATTTAGCCAA+AGG 0.550629 2.4:-3118535 MS.gene85121:CDS
CGAATCACATCCGGTCATCA+CGG 0.551333 2.4:+3118264 None:intergenic
TAGATTGCTCGAATCACATC+CGG 0.551516 2.4:+3118255 None:intergenic
AATGGAGGAGCTTATAAAGA+TGG 0.553738 2.4:-3121622 MS.gene85121:CDS
ATTCTCTTGGTCATGACTAG+TGG 0.554085 2.4:+3121722 None:intergenic
AGCAATTAGGTCTTGCTCCA+AGG 0.554546 2.4:-3122888 MS.gene85121:CDS
TAACTATGATGAATATATGA+AGG 0.554639 2.4:-3121544 MS.gene85121:CDS
TCACCAGTTGAATCCTTCAT+GGG 0.555142 2.4:+3119068 None:intergenic
GGGATATAATGTCTAATGGT+GGG 0.556948 2.4:-3118567 MS.gene85121:CDS
GGCAAATGGTAATTACTTCG+AGG 0.560541 2.4:-3118183 MS.gene85121:CDS
AATCTAGCCATACTGCCCTC+AGG 0.562964 2.4:-3118238 MS.gene85121:CDS
GAGGAAGGGAGTACAATAGG+AGG 0.565183 2.4:-3124229 MS.gene85121:CDS
TGAATACCGGTAATATCCAC+AGG 0.566945 2.4:+3118289 None:intergenic
TATGGAGGTGGAAGAAGGGG+AGG 0.567505 2.4:-3124248 MS.gene85121:CDS
GGCTTGTTGCCCCAATTGTG+GGG 0.570159 2.4:-3122624 MS.gene85121:CDS
AGAAAAGGAAGAAATATCAC+AGG 0.572043 2.4:-3124063 MS.gene85121:CDS
AATGGTAATTACTTCGAGGC+GGG 0.572459 2.4:-3118179 MS.gene85121:CDS
GGTCATCACGGATTGAATAC+CGG 0.575578 2.4:+3118276 None:intergenic
CCTCAATAATGCAACCAGAT+GGG 0.578551 2.4:+3119453 None:intergenic
GGATGTAGTAAACCAAGCAA+TGG 0.580722 2.4:-3121640 MS.gene85121:CDS
CTAAGAGAGAGAGCTGGAGA+GGG 0.582499 2.4:+3124746 None:intergenic
TTCTGAACAGATCAGAGTCA+TGG 0.585454 2.4:-3124035 MS.gene85121:CDS
ATTGTTAGGTGTTGCCACAT+TGG 0.587255 2.4:-3118947 MS.gene85121:intron
ATCAGAAATAGAGAAACTAA+GGG 0.588729 2.4:-3122681 MS.gene85121:CDS
TGGGATATAATGTCTAATGG+TGG 0.590022 2.4:-3118568 MS.gene85121:CDS
ACAAGGCTAAGAGAGAGAGC+TGG 0.592876 2.4:+3124740 None:intergenic
TCCTACAGCCAAATTAACAA+TGG 0.597793 2.4:-3118092 MS.gene85121:CDS
GCCGTTGAAAATTCAGACCA+TGG 0.602698 2.4:-3121516 MS.gene85121:CDS
TACAATAGGAGGTGAGCGTG+TGG 0.603598 2.4:-3124218 MS.gene85121:CDS
TTACAGCATCAAGAAAACAT+TGG 0.605442 2.4:+3121424 None:intergenic
TAATACATGTGTAGGTAGAG+AGG 0.607160 2.4:-3121797 MS.gene85121:intron
GACACTATATGCAAAGGAGA+AGG 0.607999 2.4:-3121208 MS.gene85121:CDS
TGTAGTAAACCAAGCAATGG+AGG 0.608774 2.4:-3121637 MS.gene85121:CDS
GATGAACTTGAAGATGATGA+AGG 0.609560 2.4:-3124133 MS.gene85121:CDS
AGAAAACATTGGACGATCCG+AGG 0.612026 2.4:+3121435 None:intergenic
AGCTAGGCCACTGTTGACAA+TGG 0.614429 2.4:+3119166 None:intergenic
GATGAAGGTGAAGATGATGA+AGG 0.621690 2.4:-3124118 MS.gene85121:CDS
AGAAACAATTGAAAATCCTG+AGG 0.621946 2.4:+3118222 None:intergenic
CACAAACAGAGATGGTGCAA+TGG 0.622132 2.4:-3122594 MS.gene85121:CDS
CAAATTAACAATGGAGTCTG+TGG 0.624759 2.4:-3118083 MS.gene85121:CDS
GAAACAATTGAAAATCCTGA+GGG 0.625241 2.4:+3118223 None:intergenic
ACGTTCGCATTGAAGTTGAA+GGG 0.628268 2.4:+3119118 None:intergenic
GTCCCCACCACAAACAGAGA+TGG 0.629482 2.4:-3122602 MS.gene85121:CDS
AATAGGAGGTGAGCGTGTGG+AGG 0.632405 2.4:-3124215 MS.gene85121:CDS
ACAGTTGACACTATATGCAA+AGG 0.638611 2.4:-3121214 MS.gene85121:CDS
TGGTTACGTAGCTTAGAGAC+CGG 0.638972 2.4:-3121579 MS.gene85121:CDS
ATCAATCCAAGATGTTCACT+AGG 0.639331 2.4:+3118775 None:intergenic
ATGTTTGCAGAATCAATGGA+AGG 0.640926 2.4:-3121269 MS.gene85121:intron
TTGGCTTTAGAAGTAGAAGG+AGG 0.644576 2.4:+3124779 None:intergenic
CTTTAAATAGGTAGTATGGG+TGG 0.647600 2.4:-3119234 MS.gene85121:intron
GCTGCCTTAGATATTAAACA+TGG 0.649260 2.4:+3121238 None:intergenic
CACATCCTGATGAAAAGCAG+AGG 0.653751 2.4:-3122924 MS.gene85121:CDS
GATGATGAAGGTGATGGTGA+TGG 0.658053 2.4:-3124106 MS.gene85121:CDS
TCTAAGCTACGTAACCACAT+TGG 0.660378 2.4:+3121585 None:intergenic
AAATGGTAATTACTTCGAGG+CGG 0.664685 2.4:-3118180 MS.gene85121:CDS
GGATGAAACTCGTCGAACTG+AGG 0.665962 2.4:-3118689 MS.gene85121:intron
AAATGCCAAACTCAAAGCCG+AGG 0.668396 2.4:-3122543 MS.gene85121:intron
GGTGAAGATGATGAAGGTGA+TGG 0.672812 2.4:-3124112 MS.gene85121:CDS
CTTTAGGGAAATATGCATCA+GGG 0.674316 2.4:-3121764 MS.gene85121:CDS
AATGGTGGTATACGCACCTG+TGG 0.675541 2.4:-3118305 MS.gene85121:CDS
AGTTGGCACAAAGAATGACA+TGG 0.675595 2.4:-3118903 MS.gene85121:CDS
TATCGCACCATTGTCAACAG+TGG 0.688962 2.4:-3119173 MS.gene85121:CDS
AAATTCTTACGAATCAATGG+TGG 0.698012 2.4:-3118320 MS.gene85121:CDS
AGATACTGCAAGCAGTTGAG+TGG 0.698583 2.4:-3119557 MS.gene85121:CDS
CAACAATGGGTGAACCAATG+TGG 0.720119 2.4:-3121599 MS.gene85121:CDS
TCATGACTAGTGGAACATGA+AGG 0.724034 2.4:+3121732 None:intergenic
TTAGCCAAAGGACAAGACAG+AGG 0.726395 2.4:-3118523 MS.gene85121:CDS
TCTGCACACATTGACAACGT+TGG 0.738320 2.4:+3124818 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr2.4 gene 3118012 3124894 3118012 ID=MS.gene85121
chr2.4 mRNA 3118012 3124894 3118012 ID=MS.gene85121.t1;Parent=MS.gene85121
chr2.4 exon 3124742 3124894 3124742 ID=MS.gene85121.t1.exon1;Parent=MS.gene85121.t1
chr2.4 CDS 3124742 3124894 3124742 ID=cds.MS.gene85121.t1;Parent=MS.gene85121.t1
chr2.4 exon 3124031 3124332 3124031 ID=MS.gene85121.t1.exon2;Parent=MS.gene85121.t1
chr2.4 CDS 3124031 3124332 3124031 ID=cds.MS.gene85121.t1;Parent=MS.gene85121.t1
chr2.4 exon 3122846 3122963 3122846 ID=MS.gene85121.t1.exon3;Parent=MS.gene85121.t1
chr2.4 CDS 3122846 3122963 3122846 ID=cds.MS.gene85121.t1;Parent=MS.gene85121.t1
chr2.4 exon 3122544 3122738 3122544 ID=MS.gene85121.t1.exon4;Parent=MS.gene85121.t1
chr2.4 CDS 3122544 3122738 3122544 ID=cds.MS.gene85121.t1;Parent=MS.gene85121.t1
chr2.4 exon 3121428 3121805 3121428 ID=MS.gene85121.t1.exon5;Parent=MS.gene85121.t1
chr2.4 CDS 3121428 3121805 3121428 ID=cds.MS.gene85121.t1;Parent=MS.gene85121.t1
chr2.4 exon 3121180 3121281 3121180 ID=MS.gene85121.t1.exon6;Parent=MS.gene85121.t1
chr2.4 CDS 3121180 3121281 3121180 ID=cds.MS.gene85121.t1;Parent=MS.gene85121.t1
chr2.4 exon 3119430 3119639 3119430 ID=MS.gene85121.t1.exon7;Parent=MS.gene85121.t1
chr2.4 CDS 3119430 3119639 3119430 ID=cds.MS.gene85121.t1;Parent=MS.gene85121.t1
chr2.4 exon 3119072 3119246 3119072 ID=MS.gene85121.t1.exon8;Parent=MS.gene85121.t1
chr2.4 CDS 3119072 3119246 3119072 ID=cds.MS.gene85121.t1;Parent=MS.gene85121.t1
chr2.4 exon 3118690 3118961 3118690 ID=MS.gene85121.t1.exon9;Parent=MS.gene85121.t1
chr2.4 CDS 3118690 3118961 3118690 ID=cds.MS.gene85121.t1;Parent=MS.gene85121.t1
chr2.4 exon 3118504 3118590 3118504 ID=MS.gene85121.t1.exon10;Parent=MS.gene85121.t1
chr2.4 CDS 3118504 3118590 3118504 ID=cds.MS.gene85121.t1;Parent=MS.gene85121.t1
chr2.4 exon 3118012 3118392 3118012 ID=MS.gene85121.t1.exon11;Parent=MS.gene85121.t1
chr2.4 CDS 3118012 3118392 3118012 ID=cds.MS.gene85121.t1;Parent=MS.gene85121.t1
Gene Sequence

>MS.gene85121

ATGACACAACCATTGCTTTTGCTCCTTAAAAAACACAACACACTTCTTTCTTCACCAACGTTGTCAATGTGTGCAGACATGTCTAATAATAATCCTCCTTCTACTTCTAAAGCCAAAGACTTCTTTCCCTCTCCAGCTCTCTCTCTTAGCCTTGTAAGTCCTAACTATAACTAACTCTCTTCTTTATTTCTTCTTCACATTTCTTCAATATTTTCTTATTTAAACGTTAATAAGTTTGTAGTTACATTAGTTTTTATGATTAAATTTTGAACCTCTAGTCAAATTTTTGTTTTTCCCCTTGTTTTTAACTCACCAACCGAGCTATCTACTTGGAACATTTCTTCCTCGTTTTATGTTTCATTTGCTTTATATGTTTCTTCTGTTAGAGTCTCACATCGGATAGATAAAATTATTTCAAGTGACACTTATGTCATTAGGGTTATTAATAATAATTTTTTTTCCTATGAGTTTTCTTTTAAAAATTTTGAGACTTTCCATCTAATGAACTAATTTTTAAGTTGGACTTTAAGTTCTAACATATTTTTTTCTTTGCTCTAATCAGGCGGGGATTTTTCGGCATGGCGGTGCGGCGGCGGGGGAGGGTGAAGGAGGGAGTTTTAGTAATATGGAGGTGGAAGAAGGGGAGGAAGGGAGTACAATAGGAGGTGAGCGTGTGGAGGAAATAAGCAGCGAGTATTCAGGTCCAACTAAATCCAAATCAATGGATGAATTTGAAGGAGATGAACTTGAAGATGATGAAGGTGAAGATGATGAAGGTGATGGTGATGGTGATAATAAGAACAAGAAGAAGAAAAGGAAGAAATATCACAGGCACACTTCTGAACAGATCAGAGTCATGGAAGCGTGCGTATAGTATAATCCACTATGCTCTTTTTTTTTTCCTCTATTCGTGTGCCTATTTTGTTTGGATGACAATTAATACTCTCCTTCATAATAGTACTACATATCACTTTAAAGAATTTTAATTTGTCTCATTCTATTTATCAATTTAAATAACAAATATACTATTAATTATTTTTTATAGTTTGTCAAAAAAAAGTATACTGATAATTAATAATTTTTAACAAGTGTGTATCATATTACTAATTTAAAAGGCAAATATCATTGAATCACCCGTGAGTGTGACTATTGAGAATTCAGTGTTATGAGTTTCTTATATTTTTTCTATTTTTAGTTCCTACTATCTTAACCTGCACAGTACTCTATGATTACCATATTGTCTGTCTTGGTTCCTTAAGAGATACCTTTGATTAATATAGTTATATTTGTTTGAATAAAAGAATGACTAGTGTGATGATTCTCTGTTCTTATTTATGTAACTTGTTTAAGTGCATAGGTCACTAATTTGAGGTTACCAATGAAATTTCTTTTTCTTCAAAGTTATATAGTATATAGTCCATGTGATTACATTTTATAATATATTGAACAGCTTTTGCTTTAATTATTTATTTGATATCTTATATATGATTATAAAAATGTTTCACACTTACTTTTTCATAGATAATCACGTACAAAAATATGATTAATTCAGTTGAAAATAAGTTAACTTAATTTGGATTGAAAATTTGGTTAACTCATATGATTGAATTAACCACAGTTTTGACGGTGAATTAAATTAAAAATGTAATCAACTAGGGTTTAGAAAAGTCAGTGAGCATGTATTATTATTGGTAAGGTTATGTCTTTGTAAAACATTTTTCCCCACTTACCACTTGATTAGTTTTTTGTTTTTTACAATACCTATTGATAAGATTGTAGTATATTTATTGAAGAGGTGTACTTGGCGTTTGCATTATTAAACACCATTTGTTGGACCACTCTACATATAAGAATTGAAGCATGTTGTGAAAAATAAATTAAATTCAATAACAAAAAAAATTTTTACATGAATGGTGAATGTAATATTGTAGGCTTTTCAAAGAGTCACCACATCCTGATGAAAAGCAGAGGCAACAACTTAGCAAGCAATTAGGTCTTGCTCCAAGGCAAGTCAAATTTTGGTTCCAAAATCGTCGTACCCAAATCAAGGTATTATTTAAATCAACTAATAAAAAAAGTGTGCTAATTTTTGCATTTTAATTATTAAGTGAATTTAACAATAATATTAAGCATTACTTATGTTGCATGTTTTTCAGGCAATACAAGAGCGACATGAAAATTCATTGTTGAAATCAGAAATAGAGAAACTAAGGGAGAAAAATAAGACCCTACGAGAGACCATAAATAAGGCTTGTTGCCCCAATTGTGGGGTCCCCACCACAAACAGAGATGGTGCAATGGCAACTGAAGAACAACAACTACGTATTGAAAATGCCAAACTCAAAGCCGAGGTTATGATTAGAAATATCTCAAACTACACATCATCACTTTTTTATTTCAATACTTAAAAAAATTAGACAAATTGTGGAGAAAACAATATATAACATTGTTGATGTAAAACAATTTTACAATCACATTCAATGAGAGACCACAATTTCTCATGTCGTATTATCAATTTCAAAATATCTTTTAAAAATGAGGTAATACGGAAAAATAATGAATTTTCATTGGATGTCGTAAAACTATTTTACATCGACAATGCATATCCTTTAAAACTATTCTACACCAACAATGCATATCTAATTTTGTTTATAGATTTATTTTGTTGCACTGGTGTAATTTTTTTTACAATGTCAACCAATCAGATGCTATAATGTGTATGATGCTTAAAAATAATCCAATAGCTATGATCGATCGACAAAGTTAAGCTAGTAGCGGTTTTGGCCTCTTGTTGGTTGATTGTAACTCATGTAACTTTATTTGAGTCCTAGCCTAGCACTTTTTATGCGGGCGTGGAGCTCCATTGATTAATATATTCCATTTTTTTTTTTGAGGAAATATATTCCTTTTTTTTTAGCTAGTACTTTATCATGTATAGTAATTTTGTATCAATGCATGTTAACCAAATCATTTTGATTATCTATAATTAGCTAGTAGTTTATCATGTCTATTAATTTTTTTTATTTTTTTTTACAATAAAAATTAAATTATAGATTGTAAAATTACACTCTTGATTAATACATGTGTAGGTAGAGAGGCTGAGAGCAGCTTTAGGGAAATATGCATCAGGGACAATGTCACCTTCATGTTCCACTAGTCATGACCAAGAGAATATTAAAAGCTCTTTGGATTTTTATACTGGAATTTTTGGTCTTGATGAGTCAAGAATAATGGATGTAGTAAACCAAGCAATGGAGGAGCTTATAAAGATGGCAACAATGGGTGAACCAATGTGGTTACGTAGCTTAGAGACCGGTCGTGAAATACTTAACTATGATGAATATATGAAGGAGTTTGCCGTTGAAAATTCAGACCATGGAAGACCAAAGAGATCCATTGAAGCTTCAAGAGATACAGGAGTTGTTTTTGCAGATCTTCCTCGGATCGTCCAATGTTTTCTTGATGCTGTAAGACATAAATTAATGCTTATACATTGAATTATCATATCCTTGAACCATATGAATAATATCATTGCATGCACATAAAAAAAAATATTATATCCTTTTGTTTTGTAGAAGTTTAACAATATTTACTTATTGTGCTATGTTTGCAGAATCAATGGAAGGAAATGTTTCCATGTTTAATATCTAAGGCAGCAACAGTTGACACTATATGCAAAGGAGAAGGTTCTAACAAGAATGGTGCAGTGCAACTGGTAAGGGCAAATGGTAGCTACTAGCTAGGTATCTTTGATATTGAATTAGATGGGCCTACTAAATTAATGAATTTCTTTTAGGTGCTTTTTTTTTTCCTACAATTCTTAAAAATTACTCTAGACCGGATTTGACTATCGGGACACGAGAAAACACTGTTTTTCTCGGATTCACTCATCTAGACACGAGAAAAAACAGCCAGTCTGAATAGGTAAATTCGAATGGGTGTAATTTTTAAAAGTATATTAGTTCATTTTTTATGAGTACCTATCAAAAAAGACATTATGAAGTTTACAAATTCACACACCAACTATTAATTAGTTAATGAAAGATTTCATAAAACCTTATATGTAGGAATGGTAAATTGAGGAAAAAAGTTTCTTATAACTTCAGTCGTTTTCATTCACTGATTTTAACTTCAAATGTCTGAAATAAAATTTATTTCTTGCTGTAAATCTGAAAGGAACAGTTCATACATGGATTTATTGATATGGATATTTTATTTTATTATAAGTGGTCCAAACATGACAAGCAAAAGAAAATGATGGTACATTGTAATTTTAAATGATATTTCCTATGTTTGAAAATTCTATTCTAATGTTGTAAATGTTTTAGTGGATAAAAAAAAGATTAAGTTGATTGTGGTAGAATTATATGCTTTTGATTATAGTATGGTTAATCTTTCTTTTTAGAAGTGAAGGTTCTTGTCAACTTTTCATCTCCTTTACTTATCTATTTCACAGAAATATAGATAAAGACACGTAAAATATAAAAAGTTCAATGTCATTTAAACTTTAGTAACACTTTGGAATTTTCCTCTCATCCATATCTTGTCTAATTCATCTCATTGGAATCTCTCGCTCTTCATGACAAATTATGATTCCATGCGGTCACTTTTTTCGCACATTCACACTATCAGGACTTTAATGGTCGTCCAATCTTGATTGGATCGTCTAGAAAAAGCAGATTTAATTTTTATTATATAATTTTTAAAATGCTGAACTTAAATCCTAACCGTTAGATCGAACGGTCACCGGTGTCCGATTGCGTGATTTGTTAACCGCAACAAATTGTGGTCCCTCCATGACTTATAAGTTATAAGTGGTGTGTATCTAACCACTTTACAAGTCAGTTTTATAAAGTTGATTTAGGTCCAATCCAAAATATAAAACCATCTGCTTTGTAGCATATATCTATATCTATGGATATATGGATATTCTAGTTTTTCACTTTTGTTTGAAAATAGTTAAATGTTGTAATTCTATAACGCAATTTAAATTAAAATTCTAGGATTAAATAAAAGTACTTTGGATTAAATACAAAATTTATAAACAACTATACATATAAAATGAGGCAGTAAGATTTATTCCATGTACAACACTTGAAGATGGAAATAGAAACAAAGGTAGAGGAAATTCTAAGATGTGGACACATTGCACAGTTTTCCAATGATAAATACTGTGGAGGCACAGAGCATATACTACTTAACTTGGACATGAAATCATAAAACTTTAACCTTAATTTTGTATTTGGTACGTACAGATGTTTGCTGAGTTGCAAATGTTAACACCAATGGTTCCAACTAGAGAAGTGTATTTTGTCAGATACTGCAAGCAGTTGAGTGGTGAAAAATGGGCTATTGTTGATGTGTCCATAGACAAAGTAGAAGACAACATTGACAAGTCCCTAGTGAAATGCAGAAAACGCCCATCTGGTTGCATTATTGAGGATAAGTCAAATGGACATTGCAAAGTATGTAATAGCAGCATTTGTTTCCATTTTTGTTACATAATGTATGGAGCATAATTTGGACCTATATGATTTTGTTTTCTTTATTCAATTTATGTGACAAGTTATATTTTTTTAACTCAATGTAAAATTCTTTTGATATTATTTTTATGAAGGTTTATTGATTTTTCCTTTGGCTTTAAATAGGTAGTATGGGTGGAGCATTTGGAGTGTCAAAAGAGTGTAGTTCATTCAATGTATCGCACCATTGTCAACAGTGGCCTAGCTTTTGGGGCAAGGCATTGGATTGCGACCCTTCAACTTCAATGCGAACGTCTAGTTTTCTTCATGGCAACAAACGTTCCCATGAAGGATTCAACTGGTGAGTAAATTAAGAATTATCATAAGAAAAAGGTTATTTTGTAAAATAGTACTTTACCAAGTAGTAAATATTGTACACTAAAATGATTAGTGATGAAAACAAATTGTTAGGTGTTGCCACATTGGCTGGAAGAAAAAGCATTTTGAAGTTGGCACAAAGAATGACATGGAGTTTCTGTCAAGCAATTAGTGCATCAAGCTTCCATACATGGACTAAGGTTACTAGTAAAACTGGAGAAGACATAAGGATTAGCTCTAGAAAGAACTTGAATGATCCTAGTGAACATCTTGGATTGATAGTTTGTGCTGTTTCTTCTATATGGTTGCCTATATCTCCTAATGTTTTGTTTGATTTCTTGAGGGATGAAACTCGTCGAACTGAGGTACCGAGTTTAATATAGTGATTCAATCATATTTTACCTGAGATATTTTGTATATGAATTTAGAGGGGATGTTTGTGTTGACAATAATCATTATGACAGTGGGATATAATGTCTAATGGTGGGACAGTGCAATCCATTGCAAATTTAGCCAAAGGACAAGACAGAGGCAATGCAGTAACTATCCAAGTAAGTTACTCAATATTCAAAACTGAACATATTGAACAAAATGCAAGTTACATTTAAAACATTTTCTAACTTCTAAGAAAAATAAGAGGTTTTTTTCTTCTCCCTTTGCAGACAATTAAATCAAAAGAAAACAATATGTGGATACTGCAAGATAGCTGCACAAATTCTTACGAATCAATGGTGGTATACGCACCTGTGGATATTACCGGTATTCAATCCGTGATGACCGGATGTGATTCGAGCAATCTAGCCATACTGCCCTCAGGATTTTCAATTGTTTCTGATGGCTTGGAATCAAGGCAAATGGTAATTACTTCGAGGCGGGAAGAGAAAAATACAGAAGGAGGATCTTTGTTTACAATAGCATTCCAAATCCTTACAAATGCTTCTCCTACAGCCAAATTAACAATGGAGTCTGTGGACTCTGTCAACTCTCTTGTATCATGTACATTGAGACACATCAAAACAAGTCTAAATTGTGAAGATGGTTAG
Protein sequence

>MS.gene85121.t1

MTQPLLLLLKKHNTLLSSPTLSMCADMSNNNPPSTSKAKDFFPSPALSLSLAGIFRHGGAAAGEGEGGSFSNMEVEEGEEGSTIGGERVEEISSEYSGPTKSKSMDEFEGDELEDDEGEDDEGDGDGDNKNKKKKRKKYHRHTSEQIRVMEALFKESPHPDEKQRQQLSKQLGLAPRQVKFWFQNRRTQIKAIQERHENSLLKSEIEKLREKNKTLRETINKACCPNCGVPTTNRDGAMATEEQQLRIENAKLKAEVERLRAALGKYASGTMSPSCSTSHDQENIKSSLDFYTGIFGLDESRIMDVVNQAMEELIKMATMGEPMWLRSLETGREILNYDEYMKEFAVENSDHGRPKRSIEASRDTGVVFADLPRIVQCFLDANQWKEMFPCLISKAATVDTICKGEGSNKNGAVQLMFAELQMLTPMVPTREVYFVRYCKQLSGEKWAIVDVSIDKVEDNIDKSLVKCRKRPSGCIIEDKSNGHCKVVWVEHLECQKSVVHSMYRTIVNSGLAFGARHWIATLQLQCERLVFFMATNVPMKDSTGVATLAGRKSILKLAQRMTWSFCQAISASSFHTWTKVTSKTGEDIRISSRKNLNDPSEHLGLIVCAVSSIWLPISPNVLFDFLRDETRRTEWDIMSNGGTVQSIANLAKGQDRGNAVTIQTIKSKENNMWILQDSCTNSYESMVVYAPVDITGIQSVMTGCDSSNLAILPSGFSIVSDGLESRQMVITSRREEKNTEGGSLFTIAFQILTNASPTAKLTMESVDSVNSLVSCTLRHIKTSLNCEDG