AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85199

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85199.t1 MTR_8g028150 57.426 303 37 4 2 213 43 344 8.76e-97 288
MS.gene85199.t1 MTR_7g116890 52.500 240 66 3 22 213 106 345 1.12e-67 214
MS.gene85199.t1 MTR_8g070820 33.971 209 103 4 40 213 163 371 2.20e-23 96.7
MS.gene85199.t1 MTR_8g070820 35.079 191 99 4 40 213 163 345 2.92e-21 90.9
MS.gene85199.t1 MTR_8g077950 62.162 74 27 1 141 213 191 264 3.22e-21 89.4
MS.gene85199.t1 MTR_8g077950 62.162 74 27 1 141 213 251 324 3.96e-21 90.1
MS.gene85199.t1 MTR_8g077950 62.162 74 27 1 141 213 249 322 4.25e-21 89.7
MS.gene85199.t1 MTR_5g028850 34.906 212 100 5 40 213 159 370 9.17e-21 89.7
MS.gene85199.t1 MTR_5g028850 33.803 213 101 5 40 213 161 372 9.76e-21 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85199.t1 AT2G46270 41.137 299 74 10 2 212 38 322 2.94e-48 162
MS.gene85199.t1 AT2G46270 41.137 299 74 10 2 212 38 322 2.94e-48 162
MS.gene85199.t1 AT2G46270 39.649 285 75 8 2 212 38 299 5.33e-40 140
MS.gene85199.t1 AT2G46270 39.649 285 75 8 2 212 38 299 5.33e-40 140
MS.gene85199.t1 AT4G01120 34.256 289 97 9 2 212 39 312 5.08e-34 125
MS.gene85199.t1 AT2G35530 35.349 215 89 7 38 213 166 369 2.91e-23 96.7
MS.gene85199.t1 AT1G32150 58.904 73 28 1 143 213 287 359 1.36e-19 86.3
MS.gene85199.t1 AT4G36730 34.078 179 87 4 42 213 132 286 4.01e-18 81.3
MS.gene85199.t1 AT4G36730 55.714 70 31 0 144 213 215 284 1.46e-17 79.7

Find 50 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTATCTTCAGGCATATTA+TGG 0.202869 2.4:-4121228 MS.gene85199:intron
TGCATTGACTGCCGAGAATT+TGG 0.257432 2.4:-4119260 MS.gene85199:CDS
GTTGGTGTCTCTATGCTTGT+AGG 0.275884 2.4:+4120867 None:intergenic
GTTGGACCTGTACTTTCTTC+AGG 0.296175 2.4:-4120057 MS.gene85199:CDS
TTTGGAAGCGGCAGTATCTT+TGG 0.307531 2.4:+4120123 None:intergenic
CGAGAATTTGGTGCTGAAAT+CGG 0.374258 2.4:-4119248 MS.gene85199:CDS
TGTGGAACACTGGTGGAAAT+AGG 0.380405 2.4:+4120000 None:intergenic
GCAGCAGCTGAGTTGAAATA+TGG 0.380639 2.4:+4121183 None:intergenic
CGATTTCAGCACCAAATTCT+CGG 0.384484 2.4:+4119249 None:intergenic
ACAGGCTGAGGCTGAAGAAT+TGG 0.391234 2.4:-4119296 MS.gene85199:intron
ATCATCTGGAAACACTGATC+AGG 0.417420 2.4:-4120841 MS.gene85199:CDS
TTTGATTGTTTCCTCCTCTC+AGG 0.425650 2.4:+4119784 None:intergenic
GCCTCCTGAAGCTTGGATGC+AGG 0.426706 2.4:-4119962 MS.gene85199:intron
GTTGCCATCGCTTCCATCAC+TGG 0.442473 2.4:+4120629 None:intergenic
AAGATCCAGGCTTAGGAAGC+AGG 0.442737 2.4:-4119746 MS.gene85199:intron
GAGACACCAACTAAATCATC+TGG 0.445527 2.4:-4120855 MS.gene85199:CDS
TTCAACTCAGCTGCTGCTCC+TGG 0.457412 2.4:-4121177 MS.gene85199:intron
TGTATCTTCAGGCATATTAT+GGG 0.464465 2.4:-4121227 MS.gene85199:intron
GAAATCGGAAATGAATGAAT+TGG 0.472002 2.4:-4119233 MS.gene85199:CDS
TTCAGGTATGACCACATCAC+TGG 0.473232 2.4:-4120040 MS.gene85199:CDS
AAACTTACAGCAGGTGAAGA+TGG 0.476285 2.4:+4120969 None:intergenic
GGAAGCGATGGCAACACTAC+AGG 0.483595 2.4:-4120621 MS.gene85199:CDS
TCATACCTGCTTCCTAAGCC+TGG 0.486648 2.4:+4119741 None:intergenic
CTGCTAGAAGATCCAGGCTT+AGG 0.486710 2.4:-4119753 MS.gene85199:CDS
AGTACCTGCATCCAAGCTTC+AGG 0.494330 2.4:+4119958 None:intergenic
ACCAGTGTTCCACAGCCTTG+TGG 0.495543 2.4:-4119991 MS.gene85199:CDS
GTGTTTCCAGATGATTTAGT+TGG 0.505094 2.4:+4120849 None:intergenic
GGCAAAACTCCACAAGGCTG+TGG 0.541143 2.4:+4119982 None:intergenic
TCATCTGGAAACACTGATCA+GGG 0.547332 2.4:-4120840 MS.gene85199:CDS
GTGAATCTGCTAGAAGATCC+AGG 0.554001 2.4:-4119759 MS.gene85199:CDS
ATTACTTGAACAGATGGAGA+AGG 0.554600 2.4:-4120175 MS.gene85199:intron
TCCACAAGGCTGTGGAACAC+TGG 0.554932 2.4:+4119990 None:intergenic
GAAGCGATGGCAACACTACA+GGG 0.570248 2.4:-4120620 MS.gene85199:CDS
TTACTTGAACAGATGGAGAA+GGG 0.571149 2.4:-4120174 MS.gene85199:intron
GATACTGAGGGTTCCAGTGA+TGG 0.573693 2.4:-4120642 MS.gene85199:CDS
GCTGAGTTGAAATATGGTAG+TGG 0.584049 2.4:+4121189 None:intergenic
TGACCACATCACTGGAACTG+AGG 0.592621 2.4:-4120032 MS.gene85199:CDS
ACAAGGCTGTGGAACACTGG+TGG 0.593279 2.4:+4119993 None:intergenic
GTTCCTCAGTTCCAGTGATG+TGG 0.596954 2.4:+4120029 None:intergenic
GGTTCCAGTGATGGAAGCGA+TGG 0.601144 2.4:-4120633 MS.gene85199:CDS
ACACTGGTGGAAATAGGCGA+AGG 0.605436 2.4:+4120006 None:intergenic
TATCAACAGTGTGGATACTG+AGG 0.618321 2.4:-4120655 MS.gene85199:intron
GTCATACCTGAAGAAAGTAC+AGG 0.620947 2.4:+4120051 None:intergenic
ACCTGCATCCAAGCTTCAGG+AGG 0.636392 2.4:+4119961 None:intergenic
GTGAGCTGAAACCTGAGAGG+AGG 0.651036 2.4:-4119795 MS.gene85199:CDS
ATCAACAGTGTGGATACTGA+GGG 0.652441 2.4:-4120654 MS.gene85199:intron
TCAGGAGGCAAAACTCCACA+AGG 0.658647 2.4:+4119976 None:intergenic
CACTGGTGGAAATAGGCGAA+GGG 0.662789 2.4:+4120007 None:intergenic
AGCGTGAGCTGAAACCTGAG+AGG 0.706426 2.4:-4119798 MS.gene85199:CDS
AAGCGATGGCAACACTACAG+GGG 0.742066 2.4:-4120619 MS.gene85199:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTACATTCAATTTTTT+TGG - chr2.4:4120545-4120564 MS.gene85199:intron 10.0%
!! AAAGAAAATGTTATCACAAA+AGG - chr2.4:4121457-4121476 MS.gene85199:intron 20.0%
!! ATAAACAATACTAGTTATTC+TGG - chr2.4:4120339-4120358 MS.gene85199:intron 20.0%
!! CCATACATAATTAGTAATAT+AGG - chr2.4:4119369-4119388 MS.gene85199:intron 20.0%
!! CCTATATTACTAATTATGTA+TGG + chr2.4:4119372-4119391 None:intergenic 20.0%
!! GTACTTATGAATTATATTCA+AGG - chr2.4:4119813-4119832 MS.gene85199:intron 20.0%
!! TAATCAGAAATTTAAAAGCA+TGG + chr2.4:4120483-4120502 None:intergenic 20.0%
!! TACATATGAATATGTTATAC+TGG - chr2.4:4119704-4119723 MS.gene85199:intron 20.0%
!! TGAAAACAACTTATAGAATA+TGG - chr2.4:4121298-4121317 MS.gene85199:intron 20.0%
!! TTAGTAATATAGGATAATTC+AGG - chr2.4:4119379-4119398 MS.gene85199:intron 20.0%
!!! AAAGCAAAAGTAGTTTTTAA+TGG + chr2.4:4119543-4119562 None:intergenic 20.0%
!!! AAGCAAAAGTAGTTTTTAAT+GGG + chr2.4:4119542-4119561 None:intergenic 20.0%
!!! TCAATTTTTTTGGTTTCATA+AGG - chr2.4:4120555-4120574 MS.gene85199:intron 20.0%
! AAAAAATCAAAACTTACAGC+AGG + chr2.4:4119852-4119871 None:intergenic 25.0%
! ATAATGATATATCAACAGTG+TGG - chr2.4:4120145-4120164 MS.gene85199:CDS 25.0%
! ATATAAACATTCAACCACAA+AGG + chr2.4:4119791-4119810 None:intergenic 25.0%
! GATTGTGACTAAAGTTAATT+TGG - chr2.4:4119231-4119250 MS.gene85199:CDS 25.0%
! GTATTAATTACTTGAACAGA+TGG - chr2.4:4120628-4120647 MS.gene85199:CDS 25.0%
! TATGTACACAGAAATATCAA+TGG + chr2.4:4119690-4119709 None:intergenic 25.0%
! TTATTGAAGCATACAACTAT+TGG + chr2.4:4119326-4119345 None:intergenic 25.0%
!! GTGATAACATTTTCTTTCAA+GGG + chr2.4:4121454-4121473 None:intergenic 25.0%
!! TATGTATGGAGTATCTAATT+TGG + chr2.4:4119358-4119377 None:intergenic 25.0%
!! TGTGATAACATTTTCTTTCA+AGG + chr2.4:4121455-4121474 None:intergenic 25.0%
!! TTAAGTTGTTTATCCAAACA+CGG - chr2.4:4121407-4121426 MS.gene85199:intron 25.0%
!! TTAATTTGGTCAAAGAAATG+TGG - chr2.4:4119245-4119264 MS.gene85199:CDS 25.0%
!!! TATTTGTCACGTGTTTTTAT+GGG - chr2.4:4121136-4121155 MS.gene85199:intron 25.0%
!!! TCTTTTAAGTGAAAAAAGCT+TGG - chr2.4:4119476-4119495 MS.gene85199:intron 25.0%
!!! TTATTTGTCACGTGTTTTTA+TGG - chr2.4:4121135-4121154 MS.gene85199:intron 25.0%
AATAAGCTGAAAAGAGCTTA+TGG + chr2.4:4121253-4121272 None:intergenic 30.0%
ACTGACACTAAAGTAATTCT+TGG - chr2.4:4120970-4120989 MS.gene85199:intron 30.0%
AGTATTGTTTATCATACCAG+TGG + chr2.4:4120331-4120350 None:intergenic 30.0%
ATGTAATTAACTCTCAAACG+AGG + chr2.4:4120535-4120554 None:intergenic 30.0%
ATGTATCTTCAGGCATATTA+TGG - chr2.4:4119581-4119600 MS.gene85199:intron 30.0%
CAATGTTATAAACAAACCAC+TGG - chr2.4:4120312-4120331 MS.gene85199:intron 30.0%
CCAATTGTTAATGCATGATA+AGG - chr2.4:4120449-4120468 MS.gene85199:intron 30.0%
CCTTATCATGCATTAACAAT+TGG + chr2.4:4120452-4120471 None:intergenic 30.0%
CTGGATTACAAATATACTTC+TGG - chr2.4:4120358-4120377 MS.gene85199:intron 30.0%
CTTAATGTATCAAACCTTTG+TGG - chr2.4:4119774-4119793 MS.gene85199:CDS 30.0%
GAAATCGGAAATGAATGAAT+TGG - chr2.4:4121576-4121595 MS.gene85199:intron 30.0%
TAAACACATATGAGACTGTT+CGG - chr2.4:4121197-4121216 MS.gene85199:CDS 30.0%
TGTATCTTCAGGCATATTAT+GGG - chr2.4:4119582-4119601 MS.gene85199:intron 30.0%
TGTTGTATGTATGTATCTTC+AGG - chr2.4:4119571-4119590 MS.gene85199:intron 30.0%
TTTAGTGTCAGTAAAAGACA+AGG + chr2.4:4120962-4120981 None:intergenic 30.0%
! TTTTCATGCTTCAATTTTCC+AGG + chr2.4:4120285-4120304 None:intergenic 30.0%
!! CAGTGGTTTGTTTATAACAT+TGG + chr2.4:4120314-4120333 None:intergenic 30.0%
!! CTGTCTTATTTATAATGACC+TGG - chr2.4:4120264-4120283 MS.gene85199:intron 30.0%
!! GTTGTTTTCATAAACTATCC+TGG + chr2.4:4121288-4121307 None:intergenic 30.0%
!! TTTTATGGGTTTGTTTGGAT+TGG - chr2.4:4121150-4121169 MS.gene85199:intron 30.0%
AGACTATAAGTGTACACAAC+TGG + chr2.4:4120246-4120265 None:intergenic 35.0%
ATTACTTGAACAGATGGAGA+AGG - chr2.4:4120634-4120653 MS.gene85199:CDS 35.0%
CGTTAAAGATCACAATCACA+AGG + chr2.4:4119291-4119310 None:intergenic 35.0%
GAATCATTTGATACCAAGAC+TGG - chr2.4:4120507-4120526 MS.gene85199:intron 35.0%
GACTATAAGTGTACACAACT+GGG + chr2.4:4120245-4120264 None:intergenic 35.0%
GTGTTTCCAGATGATTTAGT+TGG + chr2.4:4119963-4119982 None:intergenic 35.0%
TCAATGAATTGTTGACACCA+CGG + chr2.4:4120403-4120422 None:intergenic 35.0%
TTACTTGAACAGATGGAGAA+GGG - chr2.4:4120635-4120654 MS.gene85199:CDS 35.0%
! ATAGGAAGTGCAGTTAATTC+TGG + chr2.4:4120076-4120095 None:intergenic 35.0%
!! CGTGTTTTTATGGGTTTGTT+TGG - chr2.4:4121145-4121164 MS.gene85199:intron 35.0%
AAACTATCCTGGAGAGCTTA+TGG + chr2.4:4121277-4121296 None:intergenic 40.0%
AAACTTACAGCAGGTGAAGA+TGG + chr2.4:4119843-4119862 None:intergenic 40.0%
ACAATCACAAGGAACGTTGT+AGG + chr2.4:4119280-4119299 None:intergenic 40.0%
ACCAGTGCTGCAGAAAAAAA+TGG - chr2.4:4120730-4120749 MS.gene85199:intron 40.0%
ATCAACAGTGTGGATACTGA+GGG - chr2.4:4120155-4120174 MS.gene85199:CDS 40.0%
ATCATCTGGAAACACTGATC+AGG - chr2.4:4119968-4119987 MS.gene85199:CDS 40.0%
CGATTTCAGCACCAAATTCT+CGG + chr2.4:4121563-4121582 None:intergenic 40.0%
CTTATTTCCATAAGCTCTCC+AGG - chr2.4:4121267-4121286 MS.gene85199:intron 40.0%
GAAGCATACAACTATTGGTG+TGG + chr2.4:4119321-4119340 None:intergenic 40.0%
GAGACACCAACTAAATCATC+TGG - chr2.4:4119954-4119973 MS.gene85199:intron 40.0%
GTGAAAAAAGCTTGGCCAAT+CGG - chr2.4:4119484-4119503 MS.gene85199:intron 40.0%
GTGCTGCAGAAAAAAATGGT+TGG - chr2.4:4120734-4120753 MS.gene85199:intron 40.0%
TATCAACAGTGTGGATACTG+AGG - chr2.4:4120154-4120173 MS.gene85199:CDS 40.0%
TATGAACATCACAGATGACC+AGG + chr2.4:4119653-4119672 None:intergenic 40.0%
TCATCTGGAAACACTGATCA+GGG - chr2.4:4119969-4119988 MS.gene85199:CDS 40.0%
TGCGTAGTTTACGGTATTCA+GGG + chr2.4:4119912-4119931 None:intergenic 40.0%
TTTGATTGTTTCCTCCTCTC+AGG + chr2.4:4121028-4121047 None:intergenic 40.0%
! CTACAGGGGTGAGAAATTTT+TGG - chr2.4:4120204-4120223 MS.gene85199:intron 40.0%
! GATAACTGACACCGTCTTTT+TGG + chr2.4:4120707-4120726 None:intergenic 40.0%
! GCTGAGTTGAAATATGGTAG+TGG + chr2.4:4119623-4119642 None:intergenic 40.0%
! GTCATACCTGAAGAAAGTAC+AGG + chr2.4:4120761-4120780 None:intergenic 40.0%
!! CGAGAATTTGGTGCTGAAAT+CGG - chr2.4:4121561-4121580 MS.gene85199:intron 40.0%
!! CTACGAGTTTGTTGCTCTAT+TGG - chr2.4:4120106-4120125 MS.gene85199:CDS 40.0%
!!! ACCATTTTTTTCTGCAGCAC+TGG + chr2.4:4120734-4120753 None:intergenic 40.0%
ACTCGTAGAGCAAGGAACAT+AGG + chr2.4:4120094-4120113 None:intergenic 45.0%
AGCAACAAACTCGTAGAGCA+AGG + chr2.4:4120102-4120121 None:intergenic 45.0%
CAGGGGATGATGTAAAGCTT+CGG + chr2.4:4119894-4119913 None:intergenic 45.0%
CGTTCTCATCTCCTCTATAC+AGG - chr2.4:4121495-4121514 MS.gene85199:intron 45.0%
CTGAATACCGTAAACTACGC+AGG - chr2.4:4119911-4119930 MS.gene85199:intron 45.0%
CTGCGTAGTTTACGGTATTC+AGG + chr2.4:4119913-4119932 None:intergenic 45.0%
GAAAGCAATCTGTGACCGAT+TGG + chr2.4:4119502-4119521 None:intergenic 45.0%
GAACAAACCTCAGTGATAGC+AGG + chr2.4:4120040-4120059 None:intergenic 45.0%
GAGCATATAGAACCAGCGTA+TGG - chr2.4:4121222-4121241 MS.gene85199:intron 45.0%
GCGTAGTTTACGGTATTCAG+GGG + chr2.4:4119911-4119930 None:intergenic 45.0%
GGCTACATGTACATGCATCA+GGG + chr2.4:4121433-4121452 None:intergenic 45.0%
GTGAATCTGCTAGAAGATCC+AGG - chr2.4:4121050-4121069 MS.gene85199:intron 45.0%
GTTGGACCTGTACTTTCTTC+AGG - chr2.4:4120752-4120771 MS.gene85199:intron 45.0%
TATAGAACCAGCGTATGGCA+TGG - chr2.4:4121227-4121246 MS.gene85199:intron 45.0%
TCATGTCATGACTGTAACCG+TGG - chr2.4:4120383-4120402 MS.gene85199:intron 45.0%
TGTGGAACACTGGTGGAAAT+AGG + chr2.4:4120812-4120831 None:intergenic 45.0%
TTCAGCTCACGCTCATTCTA+AGG + chr2.4:4121004-4121023 None:intergenic 45.0%
TTCAGGTATGACCACATCAC+TGG - chr2.4:4120769-4120788 MS.gene85199:intron 45.0%
TTTGGAAGCGGCAGTATCTT+TGG + chr2.4:4120689-4120708 None:intergenic 45.0%
! GCAGCAGCTGAGTTGAAATA+TGG + chr2.4:4119629-4119648 None:intergenic 45.0%
! GTTGGTGTCTCTATGCTTGT+AGG + chr2.4:4119945-4119964 None:intergenic 45.0%
! TACTGCCGCTTCCAAAAAGA+CGG - chr2.4:4120693-4120712 MS.gene85199:intron 45.0%
!! TGACACCGTCTTTTTGGAAG+CGG + chr2.4:4120701-4120720 None:intergenic 45.0%
!! TGCATTGACTGCCGAGAATT+TGG - chr2.4:4121549-4121568 MS.gene85199:intron 45.0%
AAGATCCAGGCTTAGGAAGC+AGG - chr2.4:4121063-4121082 MS.gene85199:intron 50.0%
AAGCGATGGCAACACTACAG+GGG - chr2.4:4120190-4120209 MS.gene85199:intron 50.0%
ACACTGGTGGAAATAGGCGA+AGG + chr2.4:4120806-4120825 None:intergenic 50.0%
ACAGGCTGAGGCTGAAGAAT+TGG - chr2.4:4121513-4121532 MS.gene85199:intron 50.0%
AGTACCTGCATCCAAGCTTC+AGG + chr2.4:4120854-4120873 None:intergenic 50.0%
ATACCGTAAACTACGCAGGC+TGG - chr2.4:4119915-4119934 MS.gene85199:intron 50.0%
CACTGGTGGAAATAGGCGAA+GGG + chr2.4:4120805-4120824 None:intergenic 50.0%
CATCTCCTCTATACAGGCTG+AGG - chr2.4:4121501-4121520 MS.gene85199:intron 50.0%
CGGAAAACCTGCTATCACTG+AGG - chr2.4:4120030-4120049 MS.gene85199:CDS 50.0%
CTGCTAGAAGATCCAGGCTT+AGG - chr2.4:4121056-4121075 MS.gene85199:intron 50.0%
GAAGCGATGGCAACACTACA+GGG - chr2.4:4120189-4120208 MS.gene85199:intron 50.0%
GATACTGAGGGTTCCAGTGA+TGG - chr2.4:4120167-4120186 MS.gene85199:intron 50.0%
GGGCTACATGTACATGCATC+AGG + chr2.4:4121434-4121453 None:intergenic 50.0%
GTTCCTCAGTTCCAGTGATG+TGG + chr2.4:4120783-4120802 None:intergenic 50.0%
TACCGTAAACTACGCAGGCT+GGG - chr2.4:4119916-4119935 MS.gene85199:intron 50.0%
TCAGGAGGCAAAACTCCACA+AGG + chr2.4:4120836-4120855 None:intergenic 50.0%
TCATACCTGCTTCCTAAGCC+TGG + chr2.4:4121071-4121090 None:intergenic 50.0%
TGACCACATCACTGGAACTG+AGG - chr2.4:4120777-4120796 MS.gene85199:intron 50.0%
TTCAGCCTCAGCCTGTATAG+AGG + chr2.4:4121509-4121528 None:intergenic 50.0%
! GAGTTTTGCCTCCTGAAGCT+TGG - chr2.4:4120840-4120859 MS.gene85199:CDS 50.0%
!! TTGAAAGTGCTGAGCTAGCG+TGG - chr2.4:4120005-4120024 MS.gene85199:CDS 50.0%
ACAAGGCTGTGGAACACTGG+TGG + chr2.4:4120819-4120838 None:intergenic 55.0%
ACATGCATCAGGGCCGTGTT+TGG + chr2.4:4121423-4121442 None:intergenic 55.0%
ACCAGTGTTCCACAGCCTTG+TGG - chr2.4:4120818-4120837 MS.gene85199:intron 55.0%
ACCTGCATCCAAGCTTCAGG+AGG + chr2.4:4120851-4120870 None:intergenic 55.0%
AGCGTGAGCTGAAACCTGAG+AGG - chr2.4:4121011-4121030 MS.gene85199:intron 55.0%
CTTATGGCCATGCCATACGC+TGG + chr2.4:4121237-4121256 None:intergenic 55.0%
GGAAGCGATGGCAACACTAC+AGG - chr2.4:4120188-4120207 MS.gene85199:intron 55.0%
GGCAAAACTCCACAAGGCTG+TGG + chr2.4:4120830-4120849 None:intergenic 55.0%
GTCCCAGCCTGCGTAGTTTA+CGG + chr2.4:4119921-4119940 None:intergenic 55.0%
GTGAGCTGAAACCTGAGAGG+AGG - chr2.4:4121014-4121033 MS.gene85199:intron 55.0%
GTTGCCATCGCTTCCATCAC+TGG + chr2.4:4120183-4120202 None:intergenic 55.0%
TCCACAAGGCTGTGGAACAC+TGG + chr2.4:4120822-4120841 None:intergenic 55.0%
TTCAACTCAGCTGCTGCTCC+TGG - chr2.4:4119632-4119651 MS.gene85199:intron 55.0%
! CTCAAACGAGGTGCCAGTCT+TGG + chr2.4:4120523-4120542 None:intergenic 55.0%
! GGTTCCAGTGATGGAAGCGA+TGG - chr2.4:4120176-4120195 MS.gene85199:intron 55.0%
GCCTCCTGAAGCTTGGATGC+AGG - chr2.4:4120847-4120866 MS.gene85199:CDS 60.0%
! AGTGCTGAGCTAGCGTGGAG+CGG - chr2.4:4120010-4120029 MS.gene85199:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 4119174 4121657 4119174 ID=MS.gene85199
chr2.4 mRNA 4119174 4121657 4119174 ID=MS.gene85199.t1;Parent=MS.gene85199
chr2.4 exon 4121655 4121657 4121655 ID=MS.gene85199.t1.exon1;Parent=MS.gene85199.t1
chr2.4 CDS 4121655 4121657 4121655 ID=cds.MS.gene85199.t1;Parent=MS.gene85199.t1
chr2.4 exon 4121178 4121238 4121178 ID=MS.gene85199.t1.exon2;Parent=MS.gene85199.t1
chr2.4 CDS 4121178 4121238 4121178 ID=cds.MS.gene85199.t1;Parent=MS.gene85199.t1
chr2.4 exon 4120977 4120996 4120977 ID=MS.gene85199.t1.exon3;Parent=MS.gene85199.t1
chr2.4 CDS 4120977 4120996 4120977 ID=cds.MS.gene85199.t1;Parent=MS.gene85199.t1
chr2.4 exon 4120819 4120898 4120819 ID=MS.gene85199.t1.exon4;Parent=MS.gene85199.t1
chr2.4 CDS 4120819 4120898 4120819 ID=cds.MS.gene85199.t1;Parent=MS.gene85199.t1
chr2.4 exon 4120620 4120668 4120620 ID=MS.gene85199.t1.exon5;Parent=MS.gene85199.t1
chr2.4 CDS 4120620 4120668 4120620 ID=cds.MS.gene85199.t1;Parent=MS.gene85199.t1
chr2.4 exon 4119963 4120184 4119963 ID=MS.gene85199.t1.exon6;Parent=MS.gene85199.t1
chr2.4 CDS 4119963 4120184 4119963 ID=cds.MS.gene85199.t1;Parent=MS.gene85199.t1
chr2.4 exon 4119747 4119824 4119747 ID=MS.gene85199.t1.exon7;Parent=MS.gene85199.t1
chr2.4 CDS 4119747 4119824 4119747 ID=cds.MS.gene85199.t1;Parent=MS.gene85199.t1
chr2.4 exon 4119174 4119314 4119174 ID=MS.gene85199.t1.exon8;Parent=MS.gene85199.t1
chr2.4 CDS 4119174 4119314 4119174 ID=cds.MS.gene85199.t1;Parent=MS.gene85199.t1
Gene Sequence

>MS.gene85199

ATGGTTATAAGATGATACTACGATTGTGTGTGTTTTTACAATAGTTAAATGATTGATGATTGTGACTAAAGTTAATTTGGTCAAAGAAATGTGGCTTTGCTAGCCTACAACGTTCCTTGTGATTGTGATCTTTAACGAGATCGACCACACCAATAGTTGTATGCTTCAATAATTGTCAGCTCCAAATTAGATACTCCATACATAATTAGTAATATAGGATAATTCAGGAAATTCAAAGTTACTTACTATAATAAGTTTTTTTATATTATAGATTTTTTTACTTTGATTATCATTAGATAAAGTCTTTTAAGTGAAAAAAGCTTGGCCAATCGGTCACAGATTGCTTTCACTTTTGAAAAGATTTACCCATTAAAAACTACTTTTGCTTTAAAGTATCTGTTGTATGTATGTATCTTCAGGCATATTATGGGCAAAGAGTCAACATTCCACTACCATATTTCAACTCAGCTGCTGCTCCTGGTCATCTGTGATGTTCATAATTACTTTGTTGTACCATTGATATTTCTGTGTACATATGAATATGTTATACTGGTGAATTTTTACTGTTATCACTAATTAACTTCTTCGTTCTTTGATGTACTTAATGTATCAAACCTTTGTGGTTGAATGTTTATATATGTACTTATGAATTATATTCAAGGAATTCCATCTTCACCTGCTGTAAGTTTTGATTTTTTCTTAGATCACTTCAAAATTCCGAAGCTTTACATCATCCCCTGAATACCGTAAACTACGCAGGCTGGGACTCCTACAAGCATAGAGACACCAACTAAATCATCTGGAAACACTGATCAGGGTTTAATCAAAAAATTGAAAGTGCTGAGCTAGCGTGGAGCGGAAAACCTGCTATCACTGAGGTTTGTTCTATACGATGATTACCAGAATTAACTGCACTTCCTATGTTCCTTGCTCTACGAGTTTGTTGCTCTATTGGATAACTGAATAATTTTATAATGATATATCAACAGTGTGGATACTGAGGGTTCCAGTGATGGAAGCGATGGCAACACTACAGGGGTGAGAAATTTTTGGCTATTATAATTTTTTCCCAGTTGTGTACACTTATAGTCTGTCTTATTTATAATGACCTGGAAAATTGAAGCATGAAAATGTTTGCCAATGTTATAAACAAACCACTGGTATGATAAACAATACTAGTTATTCTGGATTACAAATATACTTCTGGTGTCATGTCATGACTGTAACCGTGGTGTCAACAATTCATTGAGTATGATCTTGATAAATAAATACTATCCAATTGTTAATGCATGATAAGGTGAAGTTACCATGCTTTTAAATTTCTGATTAATTTGAATCATTTGATACCAAGACTGGCACCTCGTTTGAGAGTTAATTACATTCAATTTTTTTGGTTTCATAAGGATGCATTTATCGATAAAACGTTATCTAACGTTATCTACTAGTAACTTAATGTATTAATTACTTGAACAGATGGAGAAGGGAAAACTGAGATGCAAGATAGTCAAGTTTCCAAAGATACTGCCGCTTCCAAAAAGACGGTGTCAGTTATCGCAACCAGTGCTGCAGAAAAAAATGGTTGGACCTGTACTTTCTTCAGGTATGACCACATCACTGGAACTGAGGAACCCTTCGCCTATTTCCACCAGTGTTCCACAGCCTTGTGGAGTTTTGCCTCCTGAAGCTTGGATGCAGGTACTGTAAGATTTTAGTTCTACTAATGTTTCATTTCTCACATCTAGTACATTAGAGATTCTTTTGAATCACACAACAATAATGCAGCATCCTTGTCTTTTACTGACACTAAAGTAATTCTTGGTCTGAATTCCTTAGAATGAGCGTGAGCTGAAACCTGAGAGGAGGAAACAATCAAATCGTGAATCTGCTAGAAGATCCAGGCTTAGGAAGCAGGTATGATTTGATGCAAATTTGCAATTTTTGTTTCACGTGTTCGTGTTTTTTTATTTGTCACGTGTTTTTATGGGTTTGTTTGGATTGGCTAATTTGAGCTTATCAACTAACATAAACACATATGAGACTGTTCGGAAGAGCATATAGAACCAGCGTATGGCATGGCCATAAGCTCTTTTCAGCTTATTTCCATAAGCTCTCCAGGATAGTTTATGAAAACAACTTATAGAATATGGAAACAATTTGACTTTAATTTATCTTTTGCTATAGTAGTTTATACATAAACACTTATATGATGAACATTTATGCTACAAGCACTCAGTTAAGTTGTTTATCCAAACACGGCCCTGATGCATGTACATGTAGCCCTTGAAAGAAAATGTTATCACAAAAGGAAAGTTCATGACAAACGTTCTCATCTCCTCTATACAGGCTGAGGCTGAAGAATTGGCACGAAGAGTCGATGCATTGACTGCCGAGAATTTGGTGCTGAAATCGGAAATGAATGAATTGGCTGAAAATTCTGAGAAGCTGAAGATTGAAAATGCTACATTAAAGATAATGCACACATAA
Protein sequence

>MS.gene85199.t1

MAYYGQRVNIPLPYFNSAAAPGIPSSPAAGTPTSIETPTKSSGNTDQGLIKKLKVVDTEGSSDGSDGNTTGMEKGKLRCKIVKFPKILPLPKRRCQLSQPVLQKKMVGPVLSSGMTTSLELRNPSPISTSVPQPCGVLPPEAWMQNERELKPERRKQSNRESARRSRLRKQAEAEELARRVDALTAENLVLKSEMNELAENSEKLKIENATLKIMHT