AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85247

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85247.t1 MTR_2g099490 95.965 347 12 1 1 345 1 347 0.0 694
MS.gene85247.t1 MTR_2g099490 92.464 345 12 1 1 345 1 331 0.0 662
MS.gene85247.t1 MTR_8g019540 51.765 340 119 12 23 345 7 318 9.56e-86 261
MS.gene85247.t1 MTR_2g041090 55.147 136 49 4 121 253 19 145 8.91e-40 140
MS.gene85247.t1 MTR_2g030170 52.174 115 51 2 168 282 95 205 4.95e-33 122
MS.gene85247.t1 MTR_2g030170 52.174 115 51 2 168 282 128 238 1.92e-32 121
MS.gene85247.t1 MTR_8g037270 56.311 103 40 1 144 246 117 214 1.31e-30 118
MS.gene85247.t1 MTR_3g061510 49.624 133 52 3 114 246 112 229 1.82e-30 118
MS.gene85247.t1 MTR_3g061510 49.624 133 52 3 114 246 132 249 2.35e-30 118
MS.gene85247.t1 MTR_7g106450 51.000 100 40 3 147 246 136 226 5.42e-24 100
MS.gene85247.t1 MTR_1g056530 41.844 141 67 4 108 245 95 223 6.73e-24 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85247.t1 AT1G17590 38.873 355 160 13 10 345 12 328 3.37e-56 186
MS.gene85247.t1 AT1G17590 38.873 355 160 13 10 345 12 328 3.37e-56 186
MS.gene85247.t1 AT1G17590 38.873 355 160 13 10 345 12 328 3.37e-56 186
MS.gene85247.t1 AT1G17590 38.873 355 160 13 10 345 12 328 3.37e-56 186
MS.gene85247.t1 AT1G72830 36.088 363 187 13 2 345 4 340 8.56e-50 170
MS.gene85247.t1 AT1G72830 35.989 364 187 14 2 345 4 341 3.75e-48 166
MS.gene85247.t1 AT1G54160 38.281 256 107 8 22 250 11 242 1.11e-39 142
MS.gene85247.t1 AT3G14020 37.500 328 138 16 20 319 13 301 5.38e-39 140
MS.gene85247.t1 AT3G14020 37.500 328 138 16 20 319 13 301 5.38e-39 140
MS.gene85247.t1 AT1G72830 33.978 362 171 13 2 345 4 315 2.60e-38 139
MS.gene85247.t1 AT1G30500 51.938 129 58 2 130 254 40 168 1.36e-36 130
MS.gene85247.t1 AT1G30500 52.381 126 58 2 130 254 40 164 2.92e-36 130
MS.gene85247.t1 AT2G34720 51.613 124 51 3 123 246 44 158 8.78e-33 121
MS.gene85247.t1 AT2G34720 52.941 119 47 3 128 246 14 123 2.15e-31 116
MS.gene85247.t1 AT2G34720 52.941 119 47 3 128 246 14 123 2.15e-31 116
MS.gene85247.t1 AT3G20910 45.926 135 59 4 117 251 111 231 1.66e-29 115
MS.gene85247.t1 AT5G12840 47.015 134 55 4 116 248 115 233 8.44e-29 112
MS.gene85247.t1 AT5G12840 47.015 134 55 4 116 248 115 233 8.44e-29 112
MS.gene85247.t1 AT5G12840 47.015 134 55 4 116 248 116 234 8.98e-29 112
MS.gene85247.t1 AT5G12840 47.015 134 55 4 116 248 116 234 8.98e-29 112
MS.gene85247.t1 AT5G12840 47.015 134 55 4 116 248 116 234 8.98e-29 112
MS.gene85247.t1 AT5G06510 48.214 112 46 4 149 259 9 109 5.18e-24 97.4
MS.gene85247.t1 AT5G06510 48.214 112 46 4 149 259 9 109 5.18e-24 97.4
MS.gene85247.t1 AT5G06510 47.788 113 47 4 149 260 104 205 1.19e-23 98.6
MS.gene85247.t1 AT5G06510 47.788 113 47 4 149 260 104 205 1.19e-23 98.6
MS.gene85247.t1 AT5G06510 47.788 113 47 4 149 260 104 205 1.19e-23 98.6
MS.gene85247.t1 AT5G06510 47.788 113 47 4 149 260 104 205 1.19e-23 98.6
MS.gene85247.t1 AT5G06510 47.788 113 47 4 149 260 55 156 2.32e-23 96.7
MS.gene85247.t1 AT3G05690 48.544 103 39 4 147 245 107 199 7.45e-20 89.0
MS.gene85247.t1 AT3G05690 48.544 103 39 4 147 245 144 236 1.14e-19 89.0

Find 79 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTTTGAATTCACATATA+TGG 0.180422 2.4:+4738957 None:intergenic
CACTTTGAAGCTCAAACTTT+TGG 0.192638 2.4:+4740602 None:intergenic
TTCAACCTGATCATGCATTT+TGG 0.229189 2.4:+4738602 None:intergenic
GCGAATCAAGCTTTGATTCT+TGG 0.254868 2.4:+4738691 None:intergenic
TTCATATGGCCAACAATATA+AGG 0.305273 2.4:-4739482 MS.gene85247:intron
TATAGCTGGTGGAAACATTT+TGG 0.306979 2.4:+4738658 None:intergenic
AATTCCCTTGCCATCTGATA+TGG 0.316404 2.4:-4738989 MS.gene85247:CDS
GAGAACCGAGGAGAACAATA+TGG 0.321909 2.4:-4739723 MS.gene85247:CDS
AAATTCTGACCAGCTTCTTC+AGG 0.334629 2.4:-4739040 MS.gene85247:CDS
TGCTTTGAATTCACATATAT+GGG 0.338735 2.4:+4738958 None:intergenic
TTCCAGCATTGTTCTTCTAC+TGG 0.343547 2.4:-4739758 MS.gene85247:CDS
CTCATCGGATCATCATCTAT+TGG 0.345699 2.4:-4739695 MS.gene85247:CDS
GGAGGATGGACGGTGAGATT+TGG 0.358180 2.4:+4739668 None:intergenic
GCTTCTTCAGGGCCAGCTAA+TGG 0.362306 2.4:-4739028 MS.gene85247:CDS
GGCCTACTTTCTGCTTCATA+TGG 0.362322 2.4:-4739496 MS.gene85247:CDS
CACCGTCCATCCTCCACTAA+TGG 0.364359 2.4:-4739660 MS.gene85247:CDS
TATACTTGTTTGAATTTGAA+TGG 0.374199 2.4:-4738639 MS.gene85247:CDS
TACAAATTACCTTATATTGT+TGG 0.397767 2.4:+4739473 None:intergenic
TGTCGGGACTCGTGAAGATA+TGG 0.413042 2.4:+4738774 None:intergenic
TCATCGGATCATCATCTATT+GGG 0.416852 2.4:-4739694 MS.gene85247:CDS
TATATCTGCTTACAAGTGAT+TGG 0.427209 2.4:+4739630 None:intergenic
AGCACATAACAAACTCATAA+AGG 0.430383 2.4:-4738890 MS.gene85247:CDS
CAGAAGCAGTTTCCATTAGC+TGG 0.433379 2.4:+4739016 None:intergenic
TGAAATGTTTCAGCAGCAAC+AGG 0.453392 2.4:-4738532 MS.gene85247:CDS
ATGTCACATACGGATCTCAT+AGG 0.460134 2.4:-4738557 MS.gene85247:CDS
TTCCTTCCAGTCTGAGAAGA+AGG 0.473570 2.4:+4738483 None:intergenic
AATTCTGACCAGCTTCTTCA+GGG 0.474643 2.4:-4739039 MS.gene85247:CDS
AGCAACAGGAGTTAGAGTTC+AGG 0.485902 2.4:-4738518 MS.gene85247:CDS
CAAATTCAAACAAGTATAGC+TGG 0.488784 2.4:+4738644 None:intergenic
GAATTTGAATGGAAATATGT+CGG 0.489962 2.4:-4738628 MS.gene85247:CDS
GGAATCCAAAATGCATGATC+AGG 0.490416 2.4:-4738607 MS.gene85247:CDS
CTTTAATGCATGTACATGTC+GGG 0.500813 2.4:+4738758 None:intergenic
TCGACTGGCTCATCACAATC+AGG 0.502357 2.4:-4740501 MS.gene85247:intron
GAAAGTAGGCCATTGTAGCC+TGG 0.505229 2.4:+4739508 None:intergenic
AACAATATGGGATGTCTCAT+CGG 0.507452 2.4:-4739710 MS.gene85247:CDS
AACCAGTAGAAGAACAATGC+TGG 0.515161 2.4:+4739756 None:intergenic
GCACTCCACTATGCTGATCC+AGG 0.517924 2.4:-4739526 MS.gene85247:CDS
TCAGAAGATCTTCCCCATGA+TGG 0.517971 2.4:+4740651 None:intergenic
TCTTTAATGCATGTACATGT+CGG 0.521057 2.4:+4738757 None:intergenic
TATGAGACGAAGACAGTGTC+GGG 0.522586 2.4:-4738923 MS.gene85247:CDS
AGAACCGAGGAGAACAATAT+GGG 0.525995 2.4:-4739722 MS.gene85247:CDS
AACTCAGTGCTTTACTGTTG+TGG 0.539967 2.4:+4740559 None:intergenic
AGCATTGAGAAAACGGCCAC+CGG 0.542799 2.4:+4738722 None:intergenic
ACATCCCATATTGTTCTCCT+CGG 0.546379 2.4:+4739718 None:intergenic
CAAAAGTTTGAGCTTCAAAG+TGG 0.551748 2.4:-4740601 MS.gene85247:CDS
TTCCGCCATATCAGATGGCA+AGG 0.552797 2.4:+4738984 None:intergenic
TTAGCTGGCCCTGAAGAAGC+TGG 0.554264 2.4:+4739031 None:intergenic
GTAGAAGAACAATGCTGGAA+AGG 0.555226 2.4:+4739761 None:intergenic
TTAAAGAGAGCTAGAGGTGC+CGG 0.556911 2.4:-4738741 MS.gene85247:CDS
TCATCTTCAACTCAATCGAC+TGG 0.564458 2.4:-4740516 MS.gene85247:CDS
GATTGGCTGTGATCCATTAG+TGG 0.569834 2.4:+4739647 None:intergenic
TTACTGTTGTGGCATTCTTG+TGG 0.577080 2.4:+4740570 None:intergenic
ACCCTTCTTCTCAGACTGGA+AGG 0.577435 2.4:-4738485 MS.gene85247:CDS
CTCACATGCGGACGGATAGT+CGG 0.579968 2.4:+4738415 None:intergenic
TGTAGCCTGGATCAGCATAG+TGG 0.581932 2.4:+4739521 None:intergenic
ATGATGGACATCCGAAAACA+TGG 0.581989 2.4:+4740667 None:intergenic
GATGCTTCTGATGTCACATA+CGG 0.584773 2.4:-4738567 MS.gene85247:CDS
TATGCTGATCCAGGCTACAA+TGG 0.586886 2.4:-4739517 MS.gene85247:CDS
GGTTCTTCCGCCATATCAGA+TGG 0.587888 2.4:+4738979 None:intergenic
TCCCTTGCCATCTGATATGG+CGG 0.605293 2.4:-4738986 MS.gene85247:CDS
AGTTACCCTTCTTCTCAGAC+TGG 0.606122 2.4:-4738489 MS.gene85247:CDS
ATTCAAACAAGTATAGCTGG+TGG 0.610080 2.4:+4738647 None:intergenic
GGCCATATGAAGCAGAAAGT+AGG 0.615452 2.4:+4739494 None:intergenic
TACAAAGCTGACAAGAATGT+TGG 0.616527 2.4:-4738450 MS.gene85247:CDS
TTATGAGACGAAGACAGTGT+CGG 0.626148 2.4:-4738924 MS.gene85247:CDS
ATCCATTAGTGGAGGATGGA+CGG 0.629213 2.4:+4739658 None:intergenic
GTCTTCGTCTCATAATAGCA+TGG 0.632754 2.4:+4738932 None:intergenic
CAAAGTCTCTCACATGCGGA+CGG 0.635051 2.4:+4738407 None:intergenic
TCCTTCCAGTCTGAGAAGAA+GGG 0.646710 2.4:+4738484 None:intergenic
CATGCATTAAAGAGAGCTAG+AGG 0.647089 2.4:-4738747 MS.gene85247:CDS
TGTGATCCATTAGTGGAGGA+TGG 0.649182 2.4:+4739654 None:intergenic
ATGGACATCCGAAAACATGG+TGG 0.657816 2.4:+4740670 None:intergenic
TGGACATCCGAAAACATGGT+GGG 0.659209 2.4:+4740671 None:intergenic
TCCGCCATATCAGATGGCAA+GGG 0.679431 2.4:+4738985 None:intergenic
AAGAGAGCTAGAGGTGCCGG+TGG 0.680129 2.4:-4738738 MS.gene85247:CDS
GGACATCCGAAAACATGGTG+GGG 0.683730 2.4:+4740672 None:intergenic
TTCAACATTTAAGAGAACCG+AGG 0.691304 2.4:-4739735 MS.gene85247:CDS
TGGCTGTGATCCATTAGTGG+AGG 0.701564 2.4:+4739650 None:intergenic
TCTACAAAGTCTCTCACATG+CGG 0.720564 2.4:+4738403 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGAAAAACTTAAATTATA+TGG + chr2.4:4739037-4739056 None:intergenic 10.0%
!!! TTAAGTTTTTCTTATAAAAT+TGG - chr2.4:4739043-4739062 MS.gene85247:CDS 10.0%
!!! TTTTTTATCTCTTTTAATTA+CGG - chr2.4:4739684-4739703 MS.gene85247:CDS 10.0%
!!! AATTATTTTGATTTCTTATG+AGG - chr2.4:4739980-4739999 MS.gene85247:intron 15.0%
!!! TATTTACAAGTTTTTTTTGA+AGG + chr2.4:4739810-4739829 None:intergenic 15.0%
!!! TCTATTTCTTAAATTATTAC+TGG - chr2.4:4738858-4738877 MS.gene85247:intron 15.0%
!! AAAACTTGTAAATAGATGTT+AGG - chr2.4:4739816-4739835 MS.gene85247:intron 20.0%
!! TACAAATTACCTTATATTGT+TGG + chr2.4:4739657-4739676 None:intergenic 20.0%
!! TATAAAATTGGAATTTGTCT+TGG - chr2.4:4739055-4739074 MS.gene85247:CDS 20.0%
!! TATACTTGTTTGAATTTGAA+TGG - chr2.4:4740488-4740507 MS.gene85247:intron 20.0%
!! TATATATATCTATGTTCTAG+TGG - chr2.4:4739516-4739535 MS.gene85247:CDS 20.0%
!!! TTCTCATTTTGTTATGTTAT+TGG - chr2.4:4738702-4738721 MS.gene85247:CDS 20.0%
! AGTGCAAATACTAATCAAAT+GGG + chr2.4:4739324-4739343 None:intergenic 25.0%
! CTATATATTGGTAAAAGCTA+TGG - chr2.4:4739007-4739026 MS.gene85247:CDS 25.0%
! GAATTTGAATGGAAATATGT+CGG - chr2.4:4740499-4740518 MS.gene85247:intron 25.0%
! TAATATCAATATCAACATGC+TGG + chr2.4:4739734-4739753 None:intergenic 25.0%
! TGCTGAATTACTTAATTTAG+TGG - chr2.4:4739289-4739308 MS.gene85247:intron 25.0%
!! AGACACTTTTCTCAAAATTT+TGG - chr2.4:4739562-4739581 MS.gene85247:intron 25.0%
!! GACACTTTTCTCAAAATTTT+GGG - chr2.4:4739563-4739582 MS.gene85247:intron 25.0%
!! TCAATTTTCAGTCATAACTA+TGG + chr2.4:4740028-4740047 None:intergenic 25.0%
!! TGCTTTGAATTCACATATAT+GGG + chr2.4:4740172-4740191 None:intergenic 25.0%
AAATTGGAATTTGTCTTGGA+TGG - chr2.4:4739059-4739078 MS.gene85247:CDS 30.0%
AGCACATAACAAACTCATAA+AGG - chr2.4:4740237-4740256 MS.gene85247:intron 30.0%
ATGATTTATGAAAGCCGTAA+AGG + chr2.4:4739935-4739954 None:intergenic 30.0%
CAAATTCAAACAAGTATAGC+TGG + chr2.4:4740486-4740505 None:intergenic 30.0%
CAGTGCAAATACTAATCAAA+TGG + chr2.4:4739325-4739344 None:intergenic 30.0%
CCATTTAGAGGAACAAATTA+AGG + chr2.4:4739875-4739894 None:intergenic 30.0%
GTGCAAATACTAATCAAATG+GGG + chr2.4:4739323-4739342 None:intergenic 30.0%
TACTACTGCAGTTTCTTAAT+AGG + chr2.4:4738739-4738758 None:intergenic 30.0%
TATATCTATGTTCTAGTGGA+TGG - chr2.4:4739520-4739539 MS.gene85247:CDS 30.0%
TATATCTGCTTACAAGTGAT+TGG + chr2.4:4739500-4739519 None:intergenic 30.0%
TCTTTAATGCATGTACATGT+CGG + chr2.4:4740373-4740392 None:intergenic 30.0%
TGCAAATACTAATCAAATGG+GGG + chr2.4:4739322-4739341 None:intergenic 30.0%
TTCATATGGCCAACAATATA+AGG - chr2.4:4739645-4739664 MS.gene85247:CDS 30.0%
TTGATGGGAAAATAAATGGT+TGG - chr2.4:4739193-4739212 MS.gene85247:intron 30.0%
! ATCGTTGATGGGAAAATAAA+TGG - chr2.4:4739189-4739208 MS.gene85247:intron 30.0%
! CTGCTTTGAATTCACATATA+TGG + chr2.4:4740173-4740192 None:intergenic 30.0%
! GAGTTTTCAATTTCAAGAAC+AGG - chr2.4:4738586-4738605 MS.gene85247:CDS 30.0%
!! ACACCTTTAATTTGAAGTGT+CGG + chr2.4:4738780-4738799 None:intergenic 30.0%
!! CCTTAATTTGTTCCTCTAAA+TGG - chr2.4:4739872-4739891 MS.gene85247:intron 30.0%
!! GCTTTTTAGCATTGAGAAAA+CGG + chr2.4:4740415-4740434 None:intergenic 30.0%
!! TATAAATTGACTCGCTTATG+AGG + chr2.4:4739147-4739166 None:intergenic 30.0%
!!! TTCTCAAAATTTTGGGTTTC+AGG - chr2.4:4739570-4739589 MS.gene85247:intron 30.0%
AACAATATGGGATGTCTCAT+CGG - chr2.4:4739417-4739436 MS.gene85247:intron 35.0%
AACAATATTGTTAGTGCGGT+TGG + chr2.4:4740327-4740346 None:intergenic 35.0%
AGTTATGAAGTAACGTGTCA+AGG + chr2.4:4739106-4739125 None:intergenic 35.0%
ATTATGTAAACCTACCTCCT+TGG - chr2.4:4738974-4738993 MS.gene85247:CDS 35.0%
ATTCAAACAAGTATAGCTGG+TGG + chr2.4:4740483-4740502 None:intergenic 35.0%
CAAAAGTTTGAGCTTCAAAG+TGG - chr2.4:4738526-4738545 MS.gene85247:CDS 35.0%
CAAGATATCTCATCGTTGAT+GGG - chr2.4:4739178-4739197 MS.gene85247:intron 35.0%
CTTTAATGCATGTACATGTC+GGG + chr2.4:4740372-4740391 None:intergenic 35.0%
GATTAGTATTTGCACTGTGT+TGG - chr2.4:4739328-4739347 MS.gene85247:intron 35.0%
GCACAACAATATTGTTAGTG+CGG + chr2.4:4740331-4740350 None:intergenic 35.0%
TACAAAGCTGACAAGAATGT+TGG - chr2.4:4740677-4740696 MS.gene85247:CDS 35.0%
TACACATACACAGCAGATAA+TGG + chr2.4:4739232-4739251 None:intergenic 35.0%
TCATCGGATCATCATCTATT+GGG - chr2.4:4739433-4739452 MS.gene85247:intron 35.0%
TCGAATGTAACTATGTGAGT+CGG + chr2.4:4738833-4738852 None:intergenic 35.0%
TGATTGAACAGTCCATTTAG+AGG + chr2.4:4739887-4739906 None:intergenic 35.0%
TTCAACATTTAAGAGAACCG+AGG - chr2.4:4739392-4739411 MS.gene85247:intron 35.0%
! TATAGCTGGTGGAAACATTT+TGG + chr2.4:4740472-4740491 None:intergenic 35.0%
! TTCAACCTGATCATGCATTT+TGG + chr2.4:4740528-4740547 None:intergenic 35.0%
! TTTTGGACATGGATGTTTGT+TGG + chr2.4:4738511-4738530 None:intergenic 35.0%
!! CTGTTCTTCATAGCTTTTTC+TGG - chr2.4:4738911-4738930 MS.gene85247:CDS 35.0%
!! GAAACATTTTGGTCATGGTT+TGG + chr2.4:4740461-4740480 None:intergenic 35.0%
!!! CACTTTGAAGCTCAAACTTT+TGG + chr2.4:4738528-4738547 None:intergenic 35.0%
AAAACACCAGTTGACAAGCA+CGG + chr2.4:4738684-4738703 None:intergenic 40.0%
AAATTCTGACCAGCTTCTTC+AGG - chr2.4:4740087-4740106 MS.gene85247:intron 40.0%
AACCAGTAGAAGAACAATGC+TGG + chr2.4:4739374-4739393 None:intergenic 40.0%
AACTCAGTGCTTTACTGTTG+TGG + chr2.4:4738571-4738590 None:intergenic 40.0%
AATTCCCTTGCCATCTGATA+TGG - chr2.4:4740138-4740157 MS.gene85247:intron 40.0%
AATTCTGACCAGCTTCTTCA+GGG - chr2.4:4740088-4740107 MS.gene85247:intron 40.0%
ACATCCCATATTGTTCTCCT+CGG + chr2.4:4739412-4739431 None:intergenic 40.0%
AGAACCGAGGAGAACAATAT+GGG - chr2.4:4739405-4739424 MS.gene85247:intron 40.0%
AGACATCCATACACAGACAA+CGG + chr2.4:4738893-4738912 None:intergenic 40.0%
ATGTCACATACGGATCTCAT+AGG - chr2.4:4740570-4740589 MS.gene85247:CDS 40.0%
CATGCATTAAAGAGAGCTAG+AGG - chr2.4:4740380-4740399 MS.gene85247:intron 40.0%
CTCATCGGATCATCATCTAT+TGG - chr2.4:4739432-4739451 MS.gene85247:intron 40.0%
GATGCTTCTGATGTCACATA+CGG - chr2.4:4740560-4740579 MS.gene85247:CDS 40.0%
GCAAGATATCTCATCGTTGA+TGG - chr2.4:4739177-4739196 MS.gene85247:intron 40.0%
GGAATCCAAAATGCATGATC+AGG - chr2.4:4740520-4740539 MS.gene85247:CDS 40.0%
GTAGAAGAACAATGCTGGAA+AGG + chr2.4:4739369-4739388 None:intergenic 40.0%
GTCTTCGTCTCATAATAGCA+TGG + chr2.4:4740198-4740217 None:intergenic 40.0%
TATATAGCCAGTGCTAACCA+AGG + chr2.4:4738994-4739013 None:intergenic 40.0%
TCAATATCAACATGCTGGAG+TGG + chr2.4:4739729-4739748 None:intergenic 40.0%
TCATCTTCAACTCAATCGAC+TGG - chr2.4:4738611-4738630 MS.gene85247:CDS 40.0%
TGAAATGTTTCAGCAGCAAC+AGG - chr2.4:4740595-4740614 MS.gene85247:CDS 40.0%
TGTCGATGTCTAGTCCTTTA+CGG - chr2.4:4739918-4739937 MS.gene85247:intron 40.0%
TTACTGTTGTGGCATTCTTG+TGG + chr2.4:4738560-4738579 None:intergenic 40.0%
TTATGAGACGAAGACAGTGT+CGG - chr2.4:4740203-4740222 MS.gene85247:intron 40.0%
TTCCAGCATTGTTCTTCTAC+TGG - chr2.4:4739369-4739388 MS.gene85247:intron 40.0%
TTTGCACTGTGTTGGATACT+AGG - chr2.4:4739336-4739355 MS.gene85247:intron 40.0%
! ATGTTTTCGGATGTCCATCA+TGG - chr2.4:4738462-4738481 MS.gene85247:CDS 40.0%
! GAAGCTCAAACTTTTGGACA+TGG + chr2.4:4738522-4738541 None:intergenic 40.0%
! GCACCGACACTTCAAATTAA+AGG - chr2.4:4738774-4738793 MS.gene85247:CDS 40.0%
! GCGAATCAAGCTTTGATTCT+TGG + chr2.4:4740439-4740458 None:intergenic 40.0%
! TGACACTTGTTGCTAGTTAC+AGG - chr2.4:4740045-4740064 MS.gene85247:intron 40.0%
! TGTTCTAGTGGATGGAAGTA+TGG - chr2.4:4739528-4739547 MS.gene85247:CDS 40.0%
! TGTTTTCGGATGTCCATCAT+GGG - chr2.4:4738463-4738482 MS.gene85247:CDS 40.0%
!! ATGATGGACATCCGAAAACA+TGG + chr2.4:4738463-4738482 None:intergenic 40.0%
!! GGTTAGCACTGGCTATATAT+TGG - chr2.4:4738995-4739014 MS.gene85247:CDS 40.0%
!! TGGTGGAAACATTTTGGTCA+TGG + chr2.4:4740466-4740485 None:intergenic 40.0%
AGCAACAGGAGTTAGAGTTC+AGG - chr2.4:4740609-4740628 MS.gene85247:CDS 45.0%
AGTTACCCTTCTTCTCAGAC+TGG - chr2.4:4740638-4740657 MS.gene85247:CDS 45.0%
ATACTAATCAAATGGGGGCG+TGG + chr2.4:4739317-4739336 None:intergenic 45.0%
ATCCATTAGTGGAGGATGGA+CGG + chr2.4:4739472-4739491 None:intergenic 45.0%
ATGGACATCCGAAAACATGG+TGG + chr2.4:4738460-4738479 None:intergenic 45.0%
CAACATGCTGGAGTGGAATA+CGG + chr2.4:4739722-4739741 None:intergenic 45.0%
CAATGTCCGTTGTCTGTGTA+TGG - chr2.4:4738884-4738903 MS.gene85247:CDS 45.0%
CAGAAGCAGTTTCCATTAGC+TGG + chr2.4:4740114-4740133 None:intergenic 45.0%
CATACACAGACAACGGACAT+TGG + chr2.4:4738886-4738905 None:intergenic 45.0%
GAGAACCGAGGAGAACAATA+TGG - chr2.4:4739404-4739423 MS.gene85247:intron 45.0%
GGCCATATGAAGCAGAAAGT+AGG + chr2.4:4739636-4739655 None:intergenic 45.0%
GGCCTACTTTCTGCTTCATA+TGG - chr2.4:4739631-4739650 MS.gene85247:intron 45.0%
TATGAGACGAAGACAGTGTC+GGG - chr2.4:4740204-4740223 MS.gene85247:intron 45.0%
TATGCTGATCCAGGCTACAA+TGG - chr2.4:4739610-4739629 MS.gene85247:intron 45.0%
TCAGAAGATCTTCCCCATGA+TGG + chr2.4:4738479-4738498 None:intergenic 45.0%
TCCTTCCAGTCTGAGAAGAA+GGG + chr2.4:4740646-4740665 None:intergenic 45.0%
TGGACATCCGAAAACATGGT+GGG + chr2.4:4738459-4738478 None:intergenic 45.0%
TGTGATCCATTAGTGGAGGA+TGG + chr2.4:4739476-4739495 None:intergenic 45.0%
TTAGGAGTAGATGTTGCAGC+TGG - chr2.4:4739834-4739853 MS.gene85247:intron 45.0%
TTCCTTCCAGTCTGAGAAGA+AGG + chr2.4:4740647-4740666 None:intergenic 45.0%
! GATTGGCTGTGATCCATTAG+TGG + chr2.4:4739483-4739502 None:intergenic 45.0%
! GTTTTCGGATGTCCATCATG+GGG - chr2.4:4738464-4738483 MS.gene85247:CDS 45.0%
! TCAACACCCCACCATGTTTT+CGG - chr2.4:4738449-4738468 MS.gene85247:CDS 45.0%
! TTAAAGAGAGCTAGAGGTGC+CGG - chr2.4:4740386-4740405 MS.gene85247:intron 45.0%
!!! ATAATTCATTAAATTTTAAA+AGG + chr2.4:4739773-4739792 None:intergenic 5.0%
ACCCTTCTTCTCAGACTGGA+AGG - chr2.4:4740642-4740661 MS.gene85247:CDS 50.0%
AGACACGTGTTAGTGTCCGA+AGG - chr2.4:4738804-4738823 MS.gene85247:intron 50.0%
ATAGCCAGTGCTAACCAAGG+AGG + chr2.4:4738991-4739010 None:intergenic 50.0%
CTAGACATCGACAGAGAGCA+CGG + chr2.4:4739911-4739930 None:intergenic 50.0%
GAAAGTAGGCCATTGTAGCC+TGG + chr2.4:4739622-4739641 None:intergenic 50.0%
GGACATCCGAAAACATGGTG+GGG + chr2.4:4738458-4738477 None:intergenic 50.0%
GGTTCTTCCGCCATATCAGA+TGG + chr2.4:4740151-4740170 None:intergenic 50.0%
GTGATTCCGTGCTTGTCAAC+TGG - chr2.4:4738675-4738694 MS.gene85247:CDS 50.0%
TCCCTTGCCATCTGATATGG+CGG - chr2.4:4740141-4740160 MS.gene85247:intron 50.0%
TCCGCCATATCAGATGGCAA+GGG + chr2.4:4740145-4740164 None:intergenic 50.0%
TGGCTGTGATCCATTAGTGG+AGG + chr2.4:4739480-4739499 None:intergenic 50.0%
TGTAGCCTGGATCAGCATAG+TGG + chr2.4:4739609-4739628 None:intergenic 50.0%
TGTCGGGACTCGTGAAGATA+TGG + chr2.4:4740356-4740375 None:intergenic 50.0%
TTCCGCCATATCAGATGGCA+AGG + chr2.4:4740146-4740165 None:intergenic 50.0%
! AGCATTGAGAAAACGGCCAC+CGG + chr2.4:4740408-4740427 None:intergenic 50.0%
! CTATGTGAGTCGGTGTCCTT+CGG + chr2.4:4738823-4738842 None:intergenic 50.0%
! TCGACTGGCTCATCACAATC+AGG - chr2.4:4738626-4738645 MS.gene85247:CDS 50.0%
CACCGTCCATCCTCCACTAA+TGG - chr2.4:4739467-4739486 MS.gene85247:intron 55.0%
CCAGTGCTAACCAAGGAGGT+AGG + chr2.4:4738987-4739006 None:intergenic 55.0%
CCTACCTCCTTGGTTAGCAC+TGG - chr2.4:4738984-4739003 MS.gene85247:CDS 55.0%
GCACTCCACTATGCTGATCC+AGG - chr2.4:4739601-4739620 MS.gene85247:intron 55.0%
GCTTCTTCAGGGCCAGCTAA+TGG - chr2.4:4740099-4740118 MS.gene85247:intron 55.0%
GGAGGATGGACGGTGAGATT+TGG + chr2.4:4739462-4739481 None:intergenic 55.0%
TTAGCTGGCCCTGAAGAAGC+TGG + chr2.4:4740099-4740118 None:intergenic 55.0%
!! AAGAGAGCTAGAGGTGCCGG+TGG - chr2.4:4740389-4740408 MS.gene85247:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 4738416 4740733 4738416 ID=MS.gene85247
chr2.4 mRNA 4738416 4740733 4738416 ID=MS.gene85247.t1;Parent=MS.gene85247
chr2.4 exon 4740502 4740733 4740502 ID=MS.gene85247.t1.exon1;Parent=MS.gene85247.t1
chr2.4 CDS 4740502 4740733 4740502 ID=cds.MS.gene85247.t1;Parent=MS.gene85247.t1
chr2.4 exon 4739643 4739791 4739643 ID=MS.gene85247.t1.exon2;Parent=MS.gene85247.t1
chr2.4 CDS 4739643 4739791 4739643 ID=cds.MS.gene85247.t1;Parent=MS.gene85247.t1
chr2.4 exon 4739483 4739557 4739483 ID=MS.gene85247.t1.exon3;Parent=MS.gene85247.t1
chr2.4 CDS 4739483 4739557 4739483 ID=cds.MS.gene85247.t1;Parent=MS.gene85247.t1
chr2.4 exon 4738882 4739082 4738882 ID=MS.gene85247.t1.exon4;Parent=MS.gene85247.t1
chr2.4 CDS 4738882 4739082 4738882 ID=cds.MS.gene85247.t1;Parent=MS.gene85247.t1
chr2.4 exon 4738416 4738796 4738416 ID=MS.gene85247.t1.exon5;Parent=MS.gene85247.t1
chr2.4 CDS 4738416 4738796 4738416 ID=cds.MS.gene85247.t1;Parent=MS.gene85247.t1
Gene Sequence

>MS.gene85247

ATGAAGTGCTTATGTGAGAAAGACTCTGCTCATTCAACACCCCACCATGTTTTCGGATGTCCATCATGGGGAAGATCTTCTGAATCTGAAGTCCAACAAACATCCATGTCCAAAAGTTTGAGCTTCAAAGTGGAAGCTCTACCACAAGAATGCCACAACAGTAAAGCACTGAGTTTTCAATTTCAAGAACAGGATTCATCTTCAACTCAATCGACTGGCTCATCACAATCAGGTATTTGAATTGAAACACTCTATTTGTGTGATTCCGTGCTTGTCAACTGGTGTTTTCTCATTTTGTTATGTTATTGGTGTATATATCTCCTATTAAGAAACTGCAGTAGTACTTTGTATCTATGTAGCACCGACACTTCAAATTAAAGGTGTCTTGAGACACGTGTTAGTGTCCGAAGGACACCGACTCACATAGTTACATTCGATTACTTCTATTTCTTAAATTATTACTGGTGCCAATGTCCGTTGTCTGTGTATGGATGTCTGTTCTTCATAGCTTTTTCTGGTGATTGAAACAAAATGAAGTGATAGAAGTATATGATTATGATTATGTAAACCTACCTCCTTGGTTAGCACTGGCTATATATTGGTAAAAGCTATGGTCATCCATATAATTTAAGTTTTTCTTATAAAATTGGAATTTGTCTTGGATGGCTCAATCAAAATAACAAAATACCTTGACACGTTACTTCATAACTCATATTGAGAATTCAGCTCCTCATAAGCGAGTCAATTTATAGAATAATAAAGCAAGATATCTCATCGTTGATGGGAAAATAAATGGTTGGTATTCTAACACATCCATTATCTGCTGTGTATGTGTATGTGTATGTAATAACAACAAATTTTAACATTTGAGATTGCTGAATTACTTAATTTAGTGGAGCCACGCCCCCATTTGATTAGTATTTGCACTGTGTTGGATACTAGGTCAAATTCCTTTCCAGCATTGTTCTTCTACTGGTTCAACATTTAAGAGAACCGAGGAGAACAATATGGGATGTCTCATCGGATCATCATCTATTGGGAGTCCAAATCTCACCGTCCATCCTCCACTAATGGATCACAGCCAATCACTTGTAAGCAGATATATATATCTATGTTCTAGTGGATGGAAGTATGGAACATATATGCAGACACTTTTCTCAAAATTTTGGGTTTCAGGCGCATGTTGCACTCCACTATGCTGATCCAGGCTACAATGGCCTACTTTCTGCTTCATATGGCCAACAATATAAGGTAATTTGTATTCAAGATTTTTTATCTCTTTTAATTACGGTTTATTGTGTTACCGTATTCCACTCCAGCATGTTGATATTGATATTAATATTGTATGCAGTTTCCTTTTAAAATTTAATGAATTATGATTATGATCAAATCCTTCAAAAAAAACTTGTAAATAGATGTTAGGAGTAGATGTTGCAGCTGGTACATCTTGTTTATACCTTAATTTGTTCCTCTAAATGGACTGTTCAATCATCCGTGCTCTCTGTCGATGTCTAGTCCTTTACGGCTTTCATAAATCATGATTACATCAAGACTGTTCAGCTTCAATTATTTTGATTTCTTATGAGGTGTATTAGTTCTCTAACTCGAACCATAGTTATGACTGAAAATTGACACTTGTTGCTAGTTACAGGCTTATAGCATCATGTCATCAAATTCTGACCAGCTTCTTCAGGGCCAGCTAATGGAAACTGCTTCTGTTCGAATTCCCTTGCCATCTGATATGGCGGAAGAACCCATATATGTGAATTCAAAGCAGTACCATGCTATTATGAGACGAAGACAGTGTCGGGCAAAACTTGAAGCACATAACAAACTCATAAAGGATCGTAAAGTAAGCACACATGCATTTCTGTTTTGTCTCTTCTATAGCATAAAATTTCTCTTAAACCAACCGCACTAACAATATTGTTGTGCAGCCATATCTTCACGAGTCCCGACATGTACATGCATTAAAGAGAGCTAGAGGTGCCGGTGGCCGTTTTCTCAATGCTAAAAAGCTCCAAGAATCAAAGCTTGATTCGCCAAACCATGACCAAAATGTTTCCACCAGCTATACTTGTTTGAATTTGAATGGAAATATGTCGGAATCCAAAATGCATGATCAGGTTGAAAACTACAGAGATGATGCTTCTGATGTCACATACGGATCTCATAGGAATGAAATGTTTCAGCAGCAACAGGAGTTAGAGTTCAGGTTATGCAGTTACCCTTCTTCTCAGACTGGAAGGAACATGCATGATTACAAAGCTGACAAGAATGTTGGTGCGAATCAACGCCGACTATCCGTCCGCATGTGA
Protein sequence

>MS.gene85247.t1

MKCLCEKDSAHSTPHHVFGCPSWGRSSESEVQQTSMSKSLSFKVEALPQECHNSKALSFQFQEQDSSSTQSTGSSQSGQIPFQHCSSTGSTFKRTEENNMGCLIGSSSIGSPNLTVHPPLMDHSQSLAHVALHYADPGYNGLLSASYGQQYKAYSIMSSNSDQLLQGQLMETASVRIPLPSDMAEEPIYVNSKQYHAIMRRRQCRAKLEAHNKLIKDRKPYLHESRHVHALKRARGAGGRFLNAKKLQESKLDSPNHDQNVSTSYTCLNLNGNMSESKMHDQVENYRDDASDVTYGSHRNEMFQQQQELEFRLCSYPSSQTGRNMHDYKADKNVGANQRRLSVRM