AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85274

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85274.t1 MTR_2g099010 97.531 243 6 0 1 243 1 243 0.0 497
MS.gene85274.t1 MTR_2g099010 97.119 243 6 1 1 243 1 242 5.55e-179 491
MS.gene85274.t1 MTR_2g099010 95.722 187 8 0 57 243 31 217 1.91e-123 349
MS.gene85274.t1 MTR_4g067320 70.082 244 68 3 1 243 1 240 2.04e-123 350
MS.gene85274.t1 MTR_4g067320 69.672 244 68 4 1 243 1 239 3.70e-121 345
MS.gene85274.t1 MTR_2g073370 58.333 108 45 0 1 108 1 108 6.74e-42 144
MS.gene85274.t1 MTR_1g023260 35.338 266 142 6 1 242 1 260 1.10e-36 130
MS.gene85274.t1 MTR_1g023260 41.040 173 81 2 1 152 1 173 6.92e-35 123
MS.gene85274.t1 MTR_3g113070 34.529 223 123 5 1 217 1 206 1.17e-34 124
MS.gene85274.t1 MTR_3g117320 53.211 109 50 1 1 108 1 109 8.80e-34 122
MS.gene85274.t1 MTR_4g071200 48.214 112 57 1 1 111 1 112 1.67e-32 118
MS.gene85274.t1 MTR_5g021580 46.939 98 49 2 1 98 1 95 3.67e-23 93.2
MS.gene85274.t1 MTR_5g021580 46.939 98 49 2 1 98 1 95 3.88e-23 93.2
MS.gene85274.t1 MTR_3g105710 42.708 96 46 1 5 100 6 92 2.41e-17 79.7
MS.gene85274.t1 MTR_7g018170 40.217 92 46 1 5 96 26 108 9.56e-17 79.0
MS.gene85274.t1 MTR_7g018170 50.000 50 25 0 5 54 69 118 4.48e-11 62.4
MS.gene85274.t1 MTR_1g013450 40.217 92 46 1 5 96 6 88 3.37e-16 76.6
MS.gene85274.t1 MTR_4g128930 36.957 92 49 1 5 96 22 104 3.80e-15 74.3
MS.gene85274.t1 MTR_7g108150 39.796 98 49 2 5 102 20 107 4.60e-13 68.2
MS.gene85274.t1 MTR_5g069480 33.333 96 55 1 1 96 1 87 8.12e-12 64.7
MS.gene85274.t1 MTR_5g069480 33.333 96 55 1 1 96 1 87 1.05e-11 63.9
MS.gene85274.t1 MTR_3g082630 35.417 96 52 2 1 96 1 86 6.81e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85274.t1 AT2G31380 57.317 246 94 5 1 243 1 238 2.85e-94 276
MS.gene85274.t1 AT1G06040 57.087 254 92 7 1 243 1 248 8.84e-94 276
MS.gene85274.t1 AT1G06040 62.941 170 52 4 1 161 1 168 9.18e-68 207
MS.gene85274.t1 AT1G78600 58.333 108 45 0 1 108 1 108 5.33e-41 142
MS.gene85274.t1 AT1G78600 58.333 108 45 0 1 108 1 108 9.66e-41 142
MS.gene85274.t1 AT4G10240 52.632 114 44 2 1 108 1 110 2.30e-35 124
MS.gene85274.t1 AT1G75540 52.174 115 52 1 1 112 1 115 4.75e-34 125
MS.gene85274.t1 AT4G39070 51.887 106 50 1 1 105 1 106 2.60e-30 113
MS.gene85274.t1 AT2G21320 48.980 98 47 2 1 98 1 95 1.73e-23 93.6
MS.gene85274.t1 AT4G38960 48.980 98 47 2 1 98 1 95 4.57e-23 92.8
MS.gene85274.t1 AT4G38960 48.980 98 47 2 1 98 1 95 4.57e-23 92.8
MS.gene85274.t1 AT5G15850 42.391 92 44 1 5 96 12 94 2.79e-17 80.1
MS.gene85274.t1 AT2G24790 40.625 96 48 1 5 100 8 94 9.03e-17 78.2
MS.gene85274.t1 AT2G24790 41.304 92 45 1 5 96 8 90 1.03e-16 76.6
MS.gene85274.t1 AT3G02380 30.220 182 108 5 5 182 16 182 1.17e-15 75.5
MS.gene85274.t1 AT5G24930 39.583 96 49 1 5 100 6 92 1.44e-15 75.5
MS.gene85274.t1 AT5G15840 32.520 123 71 2 5 124 20 133 5.74e-15 73.6
MS.gene85274.t1 AT5G15840 32.520 123 71 2 5 124 20 133 6.90e-15 72.8
MS.gene85274.t1 AT2G33500 34.000 100 57 1 3 102 10 100 3.11e-12 65.9
MS.gene85274.t1 AT1G28050 33.663 101 58 1 2 102 6 97 3.15e-12 65.9
MS.gene85274.t1 AT2G33500 34.000 100 57 1 3 102 10 100 3.24e-12 65.9
MS.gene85274.t1 AT2G47890 37.647 85 44 1 5 89 13 88 6.40e-11 61.6
MS.gene85274.t1 AT2G47890 37.647 85 44 1 5 89 13 88 6.99e-11 61.6

Find 42 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTGGCATATGTCACATTT+AGG 0.154164 2.4:-5074979 None:intergenic
GTGAGTTTGTTCGTCGTCTT+TGG 0.207161 2.4:-5075841 None:intergenic
TTGGGGTGTTGATGACTTAT+TGG 0.243521 2.4:+5075941 MS.gene85274:CDS
ACTTGATGTGTATTCCTCTT+AGG 0.287737 2.4:-5075873 None:intergenic
CTTGGCATATGTCACATTTA+GGG 0.307139 2.4:-5074978 None:intergenic
TGATTTGCAGAAAGGCTTCC+AGG 0.309803 2.4:-5075768 None:intergenic
TCACGTATGAGTTCAATTCT+AGG 0.311669 2.4:-5076787 None:intergenic
AGTTTCTGTGTGATGAATAA+TGG 0.342501 2.4:-5076738 None:intergenic
CAGAAGGATTCCATGCAGTT+TGG 0.346227 2.4:+5076610 MS.gene85274:intron
TTTGGCGCAGTTAGATTTCA+TGG 0.374267 2.4:-5075823 None:intergenic
TACTTTACCGTGCCTGATCT+TGG 0.385640 2.4:+5076835 MS.gene85274:CDS
TGGTTGCTAGCATGAGTTAC+TGG 0.389027 2.4:-5076718 None:intergenic
GCACAACAAATCATTGTTGC+AGG 0.412099 2.4:-5074850 None:intergenic
TTCCATGCAGTTTGGAGAGA+TGG 0.460835 2.4:+5076618 MS.gene85274:CDS
CATTTGCTTCATCCAAGATC+AGG 0.470656 2.4:-5076847 None:intergenic
AGGAACTTCAGCTGCAGCCA+TGG 0.492244 2.4:-5076689 None:intergenic
AAGTCAGGCACTTGCTGAGA+AGG 0.502751 2.4:-5075909 None:intergenic
TGATGTGTATTCCTCTTAGG+TGG 0.511604 2.4:-5075870 None:intergenic
TTTGTTAGACATGTGTGACT+TGG 0.512562 2.4:-5076761 None:intergenic
CTCACTTGGAACCACCTAAG+AGG 0.518615 2.4:+5075859 MS.gene85274:CDS
CACCAGCGCTTCCTTGCGAC+AGG 0.519290 2.4:+5075789 MS.gene85274:CDS
AGCGCTGGTGATTTGCAGAA+AGG 0.527070 2.4:-5075776 None:intergenic
TGCAGTTTGGAGAGATGGAA+TGG 0.544384 2.4:+5076623 MS.gene85274:CDS
AGTTAGATTTCATGGCCACT+TGG 0.554538 2.4:-5075815 None:intergenic
TTCCATCTCTCCAAACTGCA+TGG 0.555768 2.4:-5076620 None:intergenic
TACCTGTCGCAAGGAAGCGC+TGG 0.556206 2.4:-5075791 None:intergenic
AGACGACGAACAAACTCACT+TGG 0.565694 2.4:+5075845 MS.gene85274:CDS
GACGAATCGATTCATTTACC+TGG 0.577934 2.4:+5075750 MS.gene85274:CDS
GATGGAATGGTTCACAGAGG+AGG 0.579999 2.4:+5076636 MS.gene85274:CDS
ATGGAATGGTTCACAGAGGA+GGG 0.583205 2.4:+5076637 MS.gene85274:CDS
AGCTTGCAAGCAAGCATCAG+AGG 0.588673 2.4:+5074926 MS.gene85274:CDS
TGACTTCACTCAAGAAGCCA+TGG 0.595191 2.4:+5076672 MS.gene85274:CDS
AATAAGTCATCAACACCCCA+AGG 0.619248 2.4:-5075939 None:intergenic
GCTTCATCCAAGATCAGGCA+CGG 0.625901 2.4:-5076842 None:intergenic
CCACTTGGATACCTGTCGCA+AGG 0.630313 2.4:-5075800 None:intergenic
ATAAGTCATCAACACCCCAA+GGG 0.636431 2.4:-5075938 None:intergenic
GCATGAGTTACTGGAAGCTG+AGG 0.655267 2.4:-5076709 None:intergenic
TAAGTCATCAACACCCCAAG+GGG 0.667753 2.4:-5075937 None:intergenic
AGACAGGGCACTCTTCTGTG+AGG 0.698154 2.4:+5075722 MS.gene85274:CDS
CCTTGCGACAGGTATCCAAG+TGG 0.753321 2.4:+5075800 MS.gene85274:CDS
AAGTCATCAACACCCCAAGG+GGG 0.775594 2.4:-5075936 None:intergenic
AGAGATGGAATGGTTCACAG+AGG 0.780922 2.4:+5076633 MS.gene85274:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATAAACTCAAATAAAT+CGG + chr2.4:5075558-5075577 MS.gene85274:intron 10.0%
!! AAATTATAAAGTATACTTTC+TGG + chr2.4:5075205-5075224 MS.gene85274:intron 15.0%
!! TTTCTTATCTTAATAAGATT+AGG + chr2.4:5075256-5075275 MS.gene85274:intron 15.0%
!!! AGACAAAAAAACATTTTATA+AGG - chr2.4:5076105-5076124 None:intergenic 15.0%
!! AAAAAACAGAGAGAAAATTA+AGG - chr2.4:5075017-5075036 None:intergenic 20.0%
!! ACATAATAAGTAACAGTTAT+AGG - chr2.4:5075636-5075655 None:intergenic 20.0%
!! ACATTTCTTGTATTTAATCA+TGG - chr2.4:5076528-5076547 None:intergenic 20.0%
!! ATCATGTATCATTAGATAAT+AGG - chr2.4:5076352-5076371 None:intergenic 20.0%
!! ATCTAATGATACATGATTAA+GGG + chr2.4:5076355-5076374 MS.gene85274:intron 20.0%
!! CATTTCTTGTATTTAATCAT+GGG - chr2.4:5076527-5076546 None:intergenic 20.0%
!! TATCTAATGATACATGATTA+AGG + chr2.4:5076354-5076373 MS.gene85274:intron 20.0%
!! TGAAATCGAATATTTAACTA+AGG - chr2.4:5076067-5076086 None:intergenic 20.0%
!!! TTACAAAACTACATGTTTTT+AGG - chr2.4:5076138-5076157 None:intergenic 20.0%
!!! TTACAAATTTTGTTAGTTCA+TGG - chr2.4:5076039-5076058 None:intergenic 20.0%
!!! TTGCAACTTTATTTCATAAT+TGG + chr2.4:5076000-5076019 MS.gene85274:intron 20.0%
!!! TTTTGTTATAATTGAGCTAA+GGG - chr2.4:5076319-5076338 None:intergenic 20.0%
! ACTATTTACATAAGTCATTC+TGG + chr2.4:5075488-5075507 MS.gene85274:intron 25.0%
! CTTAGCTCAATTATAACAAA+AGG + chr2.4:5076318-5076337 MS.gene85274:intron 25.0%
! TGAAGCACAATAAGAAAAAA+AGG - chr2.4:5076592-5076611 None:intergenic 25.0%
! TTAATCATGGGAAAATACTT+GGG - chr2.4:5076515-5076534 None:intergenic 25.0%
! TTCATACATGAGTAACTAAT+AGG + chr2.4:5075105-5075124 MS.gene85274:intron 25.0%
! TTTAATCATGGGAAAATACT+TGG - chr2.4:5076516-5076535 None:intergenic 25.0%
!! AATCCTAAAGTGTTTAAAAG+AGG - chr2.4:5075378-5075397 None:intergenic 25.0%
!! TAACCTCTTTTAAACACTTT+AGG + chr2.4:5075372-5075391 MS.gene85274:intron 25.0%
!! TAAGGTGCTAATTAATTACT+TGG - chr2.4:5074999-5075018 None:intergenic 25.0%
!!! CTTTTGTTATAATTGAGCTA+AGG - chr2.4:5076320-5076339 None:intergenic 25.0%
AAAGTTAAGACAACATACCT+TGG - chr2.4:5075135-5075154 None:intergenic 30.0%
AGTTTCTGTGTGATGAATAA+TGG - chr2.4:5076741-5076760 None:intergenic 30.0%
ATAAAACAACAAAGACAGCT+AGG + chr2.4:5076222-5076241 MS.gene85274:intron 30.0%
GTACGAACTAAACTAAAATG+TGG + chr2.4:5076161-5076180 MS.gene85274:intron 30.0%
TAAAACAACAAAGACAGCTA+GGG + chr2.4:5076223-5076242 MS.gene85274:intron 30.0%
! CATATTTTGTGTTGAAGACA+GGG + chr2.4:5075707-5075726 MS.gene85274:CDS 30.0%
! TCATATTTTGTGTTGAAGAC+AGG + chr2.4:5075706-5075725 MS.gene85274:CDS 30.0%
! TTTTCTTATTGTGCTTCAGA+AGG + chr2.4:5076594-5076613 MS.gene85274:intron 30.0%
AAGTAGCATGTATGAGTTAG+TGG + chr2.4:5075290-5075309 MS.gene85274:intron 35.0%
ACTTGATGTGTATTCCTCTT+AGG - chr2.4:5075876-5075895 None:intergenic 35.0%
ACTTGGCATATGTCACATTT+AGG - chr2.4:5074982-5075001 None:intergenic 35.0%
AGTAACTAATAGGTCATCCA+AGG + chr2.4:5075115-5075134 MS.gene85274:intron 35.0%
AGTAGCATGTATGAGTTAGT+GGG + chr2.4:5075291-5075310 MS.gene85274:intron 35.0%
ATTAAATCTGGGTCACACAA+AGG - chr2.4:5076472-5076491 None:intergenic 35.0%
CTTGGCATATGTCACATTTA+GGG - chr2.4:5074981-5075000 None:intergenic 35.0%
TCACGTATGAGTTCAATTCT+AGG - chr2.4:5076790-5076809 None:intergenic 35.0%
TGTATGAGTTAGTGGGAATT+AGG + chr2.4:5075298-5075317 MS.gene85274:intron 35.0%
TTTGTTAGACATGTGTGACT+TGG - chr2.4:5076764-5076783 None:intergenic 35.0%
!! CAATTGTCTAAGAGCATCTT+TGG - chr2.4:5075585-5075604 None:intergenic 35.0%
AATAAGTCATCAACACCCCA+AGG - chr2.4:5075942-5075961 None:intergenic 40.0%
AGTTAGATTTCATGGCCACT+TGG - chr2.4:5075818-5075837 None:intergenic 40.0%
ATAAGTCATCAACACCCCAA+GGG - chr2.4:5075941-5075960 None:intergenic 40.0%
CTTCACCTGTATGTACAAAC+AGG + chr2.4:5075671-5075690 MS.gene85274:intron 40.0%
CTTGTCCTGTTTGTACATAC+AGG - chr2.4:5075679-5075698 None:intergenic 40.0%
GCACAACAAATCATTGTTGC+AGG - chr2.4:5074853-5074872 None:intergenic 40.0%
GTACATACAGGTGAAGCAAA+AGG - chr2.4:5075667-5075686 None:intergenic 40.0%
GTCACCGTGACATTAAATCT+GGG - chr2.4:5076417-5076398 None:intergenic 40.0%
GTGACCCAGATTTAATGTCA+CGG + chr2.4:5076476-5076495 MS.gene85274:intron 40.0%
GTGGCTCAGATTTAATGTCA+CGG - chr2.4:5076405-5076424 None:intergenic 40.0%
TGATGTGTATTCCTCTTAGG+TGG - chr2.4:5075873-5075892 None:intergenic 40.0%
TGTCACCGTGACATTAAATC+TGG - chr2.4:5076416-5076397 None:intergenic 40.0%
TTTGGCGCAGTTAGATTTCA+TGG - chr2.4:5075826-5075845 None:intergenic 40.0%
! GACGAATCGATTCATTTACC+TGG + chr2.4:5075750-5075769 MS.gene85274:CDS 40.0%
!! ACATGATTAAGGGAAGCACT+TGG + chr2.4:5076365-5076384 MS.gene85274:intron 40.0%
!! CATGATTAAGGGAAGCACTT+GGG + chr2.4:5076366-5076385 MS.gene85274:intron 40.0%
!! TTGGGGTGTTGATGACTTAT+TGG + chr2.4:5075941-5075960 MS.gene85274:CDS 40.0%
AGACGACGAACAAACTCACT+TGG + chr2.4:5075845-5075864 MS.gene85274:CDS 45.0%
AGAGATGGAATGGTTCACAG+AGG + chr2.4:5076633-5076652 MS.gene85274:CDS 45.0%
ATGGAATGGTTCACAGAGGA+GGG + chr2.4:5076637-5076656 MS.gene85274:CDS 45.0%
CAGAAGGATTCCATGCAGTT+TGG + chr2.4:5076610-5076629 MS.gene85274:intron 45.0%
TAAGTCATCAACACCCCAAG+GGG - chr2.4:5075940-5075959 None:intergenic 45.0%
TACTTTACCGTGCCTGATCT+TGG + chr2.4:5076835-5076854 MS.gene85274:CDS 45.0%
TGACTTCACTCAAGAAGCCA+TGG + chr2.4:5076672-5076691 MS.gene85274:CDS 45.0%
TGCAGTTTGGAGAGATGGAA+TGG + chr2.4:5076623-5076642 MS.gene85274:CDS 45.0%
TGGTTGCTAGCATGAGTTAC+TGG - chr2.4:5076721-5076740 None:intergenic 45.0%
TTCCATCTCTCCAAACTGCA+TGG - chr2.4:5076623-5076642 None:intergenic 45.0%
TTCCATGCAGTTTGGAGAGA+TGG + chr2.4:5076618-5076637 MS.gene85274:CDS 45.0%
! CTTTCTGGAGATGTAGTGTG+AGG + chr2.4:5075220-5075239 MS.gene85274:intron 45.0%
! GTGAGTTTGTTCGTCGTCTT+TGG - chr2.4:5075844-5075863 None:intergenic 45.0%
! TGATTTGCAGAAAGGCTTCC+AGG - chr2.4:5075771-5075790 None:intergenic 45.0%
! TTCACAGAGGAGGGTCTTTT+TGG + chr2.4:5076646-5076665 MS.gene85274:CDS 45.0%
!! CACAAAGGTGTTGTGTTGTG+TGG - chr2.4:5076457-5076476 None:intergenic 45.0%
AAGTCATCAACACCCCAAGG+GGG - chr2.4:5075939-5075958 None:intergenic 50.0%
ACACATTGCCACACAGCATC+TGG + chr2.4:5076430-5076449 MS.gene85274:intron 50.0%
AGCTTGCAAGCAAGCATCAG+AGG + chr2.4:5074926-5074945 MS.gene85274:CDS 50.0%
CACATTGCCACACAGCATCT+GGG + chr2.4:5076431-5076450 MS.gene85274:intron 50.0%
CTCACTTGGAACCACCTAAG+AGG + chr2.4:5075859-5075878 MS.gene85274:CDS 50.0%
GATGGAATGGTTCACAGAGG+AGG + chr2.4:5076636-5076655 MS.gene85274:CDS 50.0%
GCATGAGTTACTGGAAGCTG+AGG - chr2.4:5076712-5076731 None:intergenic 50.0%
GTGTGGCAATGTGTGTTGTG+TGG - chr2.4:5076424-5076443 None:intergenic 50.0%
! AAGTCAGGCACTTGCTGAGA+AGG - chr2.4:5075912-5075931 None:intergenic 50.0%
!! AGCGCTGGTGATTTGCAGAA+AGG - chr2.4:5075779-5075798 None:intergenic 50.0%
AGGAACTTCAGCTGCAGCCA+TGG - chr2.4:5076692-5076711 None:intergenic 55.0%
CCACTTGGATACCTGTCGCA+AGG - chr2.4:5075803-5075822 None:intergenic 55.0%
CCTGACTTTACACCCCCTTG+GGG + chr2.4:5075924-5075943 MS.gene85274:CDS 55.0%
CCTTGCGACAGGTATCCAAG+TGG + chr2.4:5075800-5075819 MS.gene85274:CDS 55.0%
GCCTGACTTTACACCCCCTT+GGG + chr2.4:5075923-5075942 MS.gene85274:CDS 55.0%
TGCCTGACTTTACACCCCCT+TGG + chr2.4:5075922-5075941 MS.gene85274:CDS 55.0%
! AGACAGGGCACTCTTCTGTG+AGG + chr2.4:5075722-5075741 MS.gene85274:CDS 55.0%
TGTGTGGCCCAGATGCTGTG+TGG - chr2.4:5076441-5076460 None:intergenic 60.0%
! CCCCAAGGGGGTGTAAAGTC+AGG - chr2.4:5075927-5075946 None:intergenic 60.0%
! TACCTGTCGCAAGGAAGCGC+TGG - chr2.4:5075794-5075813 None:intergenic 60.0%
CACCAGCGCTTCCTTGCGAC+AGG + chr2.4:5075789-5075808 MS.gene85274:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 5074817 5076861 5074817 ID=MS.gene85274
chr2.4 mRNA 5074817 5076861 5074817 ID=MS.gene85274.t1;Parent=MS.gene85274
chr2.4 exon 5074817 5074999 5074817 ID=MS.gene85274.t1.exon1;Parent=MS.gene85274.t1
chr2.4 CDS 5074817 5074999 5074817 ID=cds.MS.gene85274.t1;Parent=MS.gene85274.t1
chr2.4 exon 5075693 5075992 5075693 ID=MS.gene85274.t1.exon2;Parent=MS.gene85274.t1
chr2.4 CDS 5075693 5075992 5075693 ID=cds.MS.gene85274.t1;Parent=MS.gene85274.t1
chr2.4 exon 5076613 5076861 5076613 ID=MS.gene85274.t1.exon3;Parent=MS.gene85274.t1
chr2.4 CDS 5076613 5076861 5076613 ID=cds.MS.gene85274.t1;Parent=MS.gene85274.t1
Gene Sequence

>MS.gene85274

ATGAAAATTCAGTGTGATGTGTGTGAGAAAGCTCCTGCAACAATGATTTGTTGTGCTGATGAAGCTGCTTTGTGTGCTAAATGTGATATTGAAGTTCATGCTGCTAACAAGCTTGCAAGCAAGCATCAGAGGATTCATCTTCAATCTCTCTCTAACAAGCTCCCTAAATGTGACATATGCCAAGTAATTAATTAGCACCTTAATTTTCTCTCTGTTTTTTTGTATCATGTCATAAAATTAATCATGTTTCAATTGAGTGAATTTTTGTGATAGATATGATATTCAATATTCATACATGAGTAACTAATAGGTCATCCAAGGTATGTTGTCTTAACTTTTAAACTACTTCACAATTAATTCTAAAATAGTTTAGTTTGTTTCTTTTAAAAAATTATAAAGTATACTTTCTGGAGATGTAGTGTGAGGATGTTGCTTTCTGTTTCTTATCTTAATAAGATTAGGATGTTAATTTCAAGTAGCATGTATGAGTTAGTGGGAATTAGGAGCTTATTTTGAAAGTAGCTTATGTGAATGAGATGCTATAAGTTATTTTTATAACCTCTTTTAAACACTTTAGGATTTGCATGCACTTATCATACGAGTTTGTTTATGTAAAAGTTATTTCTATAATAAAAGATAAAATAGTCAAATTATTCTCATACAATTTATAAACTATTTACATAAGTCATTCTGGAGAGCTTATGAAAATAAGCTGAAGTTGAAGACTACTTATATACATATTATAATAAACTCAAATAAATCGGTCCAAAGATGCTCTTAGACAATTGTTTATCTGATGAATCTTGTATTAACTTTCCTATAACTGTTACTTATTATGTTCATTCTTCCTTTTGCTTCACCTGTATGTACAAACAGGACAAGACAGCTTTCATATTTTGTGTTGAAGACAGGGCACTCTTCTGTGAGGATTGTGACGAATCGATTCATTTACCTGGAAGCCTTTCTGCAAATCACCAGCGCTTCCTTGCGACAGGTATCCAAGTGGCCATGAAATCTAACTGCGCCAAAGACGACGAACAAACTCACTTGGAACCACCTAAGAGGAATACACATCAAGTTTCTCTTGAAACTCCTTCTCAGCAAGTGCCTGACTTTACACCCCCTTGGGGTGTTGATGACTTATTGGAGTTAGCAGACTTTAATTCTCATGACAAAGTAAGACTTGCAACTTTATTTCATAATTGGAAGTCAATATATGCCATGAACTAACAAAATTTGTAACATGTCCTTAGTTAAATATTCGATTTCAAATTTTGTTTTTAGTCCTTATAAAATGTTTTTTTGTCTCTTTTTAGAGCCTAAAAACATGTAGTTTTGTAATTTGTACGAACTAAACTAAAATGTGGTACATGTTAACAATATAGAGATTCAGAGAGAAGATAGAATAAAACAACAAAGACAGCTAGGGTTTCATTAGATTGACAAAAGTTCAAAATACAATAAGAATATATATAGTAAAATATAAAGAGACTAAACTACCCTTAGCTCAATTATAACAAAAGGTTCAATAACCTATTATCTAATGATACATGATTAAGGGAAGCACTTGGGTGACATAATGTCACCGTGACATTAAATCTGAGCCACACAACACACATTGCCACACAGCATCTGGGCCACACAACACAACACCTTTGTGTGACCCAGATTTAATGTCACGGTGACATTATGTCACCCAAGTATTTTCCCATGATTAAATACAAGAAATGTAAATTTTTAGTAACTAGACATATTTAACTCTATTTTTAACTCCTTTTTTTCTTATTGTGCTTCAGAAGGATTCCATGCAGTTTGGAGAGATGGAATGGTTCACAGAGGAGGGTCTTTTTGGTGATGACTTCACTCAAGAAGCCATGGCTGCAGCTGAAGTTCCTCAGCTTCCAGTAACTCATGCTAGCAACCATTATTCATCACACAGAAACTCCAAGTCACACATGTCTAACAAAAAGCCTAGAATTGAACTCATACGTGATTATGATTATGATGATGAAGATGAGTACTTTACCGTGCCTGATCTTGGATGA
Protein sequence

>MS.gene85274.t1

MKIQCDVCEKAPATMICCADEAALCAKCDIEVHAANKLASKHQRIHLQSLSNKLPKCDICQDKTAFIFCVEDRALFCEDCDESIHLPGSLSANHQRFLATGIQVAMKSNCAKDDEQTHLEPPKRNTHQVSLETPSQQVPDFTPPWGVDDLLELADFNSHDKKDSMQFGEMEWFTEEGLFGDDFTQEAMAAAEVPQLPVTHASNHYSSHRNSKSHMSNKKPRIELIRDYDYDDEDEYFTVPDLG