AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85299

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85299.t1 MTR_2g045070 84.701 451 29 4 1 415 148 594 0.0 755
MS.gene85299.t1 MTR_3g062560 90.426 94 7 1 1 94 100 191 1.73e-53 177
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85299.t1 AT1G43245 40.898 401 188 11 12 390 134 507 4.47e-81 260
MS.gene85299.t1 AT1G43245 40.898 401 188 11 12 390 134 507 6.63e-81 259

Find 60 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACTCCTCACAATCAATTT+TGG 0.137975 2.4:+45357780 None:intergenic
CAACGACGGTGCAGCGATTC+TGG 0.215687 2.4:-45358944 MS.gene85299:CDS
GTCTCTGAAGAACTTGTAAT+TGG 0.243344 2.4:+45355925 None:intergenic
CGCGCTGGTGATTTATTATC+TGG 0.254941 2.4:-45355686 MS.gene85299:CDS
GAGTATCAAGAGAATTAAAA+AGG 0.260792 2.4:-45357763 MS.gene85299:CDS
CTTACTCCATCCTGAACTTC+TGG 0.315654 2.4:+45355490 None:intergenic
GAATGGAGCTATGCGAAGTT+TGG 0.333038 2.4:+45358735 None:intergenic
TAAGTTTCTTAATGTTGATT+TGG 0.342753 2.4:-45355471 MS.gene85299:CDS
TTAACTCTTACCAGAAGTTC+AGG 0.346116 2.4:-45355500 MS.gene85299:CDS
AAGTAGAGAAGAAGAAGAAT+TGG 0.349685 2.4:+45358768 None:intergenic
TGCAAGGACCCTATCAATTT+CGG 0.353888 2.4:-45355248 MS.gene85299:CDS
ATTACCCAGCCGAAATTGAT+AGG 0.361279 2.4:+45355239 None:intergenic
CGGGCTCATGATATCAAAAC+TGG 0.384552 2.4:-45355191 MS.gene85299:intron
CAGACATTACTCGTAATGTT+TGG 0.393354 2.4:-45355307 MS.gene85299:CDS
GAGAATCGGTAGCACGCATT+AGG 0.437091 2.4:+45358796 None:intergenic
AAGAATTGGAGAAGGAGAAT+CGG 0.451919 2.4:+45358782 None:intergenic
GTTGTCTGTGGAGATTCGTC+TGG 0.452765 2.4:-45355956 MS.gene85299:CDS
ATTTAGAGAGAGGTGGTGAA+GGG 0.459642 2.4:+45355742 None:intergenic
TATAACATCTTCGATGGAAT+CGG 0.473689 2.4:+45355883 None:intergenic
AATTTAGAGAGAGGTGGTGA+AGG 0.475466 2.4:+45355741 None:intergenic
GAACGAGACAATCGGAACTA+TGG 0.481166 2.4:-45356239 MS.gene85299:CDS
AGGACCCTATCAATTTCGGC+TGG 0.493252 2.4:-45355244 MS.gene85299:CDS
GATAATGAAGGATGCGCACT+CGG 0.495847 2.4:-45358868 MS.gene85299:CDS
TCATATCAAAAGCTTTCGAT+TGG 0.502770 2.4:+45355642 None:intergenic
CTCTCTGCCCTTCACATACG+TGG 0.515200 2.4:-45356178 MS.gene85299:CDS
TGTCAGAAGCAAGGTTTGAA+AGG 0.521753 2.4:+45355447 None:intergenic
GGTGTAGACGAGCAGCTAGA+AGG 0.527981 2.4:-45355794 MS.gene85299:CDS
CGGAACTATGGTCTAAGTAT+CGG 0.529829 2.4:-45356227 MS.gene85299:CDS
ACGTTGTGCAAATTCACTAC+TGG 0.536829 2.4:+45358583 None:intergenic
AGAAGAAGAAGAATTGGAGA+AGG 0.538190 2.4:+45358774 None:intergenic
ATATCAGAGTACCTTTCAGT+TGG 0.546568 2.4:-45355863 MS.gene85299:CDS
CTCTGATATAACATCTTCGA+TGG 0.555752 2.4:+45355877 None:intergenic
TTTAGAGAGAGGTGGTGAAG+GGG 0.570529 2.4:+45355743 None:intergenic
TTCATTATCATGCACCTCCA+CGG 0.572313 2.4:+45358882 None:intergenic
TTTATGATCCACGTATGTGA+AGG 0.575433 2.4:+45356170 None:intergenic
ATAATGAAGGATGCGCACTC+GGG 0.578764 2.4:-45358867 MS.gene85299:CDS
CTCTTACCAGAAGTTCAGGA+TGG 0.580229 2.4:-45355496 MS.gene85299:CDS
TACTGACCTCTTGCAGCCAA+AGG 0.583268 2.4:-45357718 MS.gene85299:intron
TGCATACCTTTGGCTGCAAG+AGG 0.584254 2.4:+45357712 None:intergenic
CTCGAGAAGATTCTCATCGA+AGG 0.597081 2.4:-45355815 MS.gene85299:CDS
AGGATGCGCACTCGGGATTG+CGG 0.598412 2.4:-45358860 MS.gene85299:CDS
ATGCAGAATCACCAACTGAA+AGG 0.598509 2.4:+45355852 None:intergenic
AGGGCAGAGAGCTACAACGT+TGG 0.602461 2.4:+45356190 None:intergenic
TTATGATCCACGTATGTGAA+GGG 0.607337 2.4:+45356171 None:intergenic
TCATATAGCAATTTAGAGAG+AGG 0.611256 2.4:+45355732 None:intergenic
GCCGCAGTAGAAAAGCAAAA+CGG 0.617046 2.4:-45358973 MS.gene85299:CDS
GGACCCTATCAATTTCGGCT+GGG 0.618597 2.4:-45355243 MS.gene85299:CDS
TCAGCATATAAAGTCCGCGC+TGG 0.621347 2.4:-45355701 MS.gene85299:CDS
CAAAACGGTTGCAGCAACGA+CGG 0.625848 2.4:-45358958 MS.gene85299:CDS
TTACCCAGCCGAAATTGATA+GGG 0.626489 2.4:+45355240 None:intergenic
AAAGAAGGACGGGTGATCTG+TGG 0.627550 2.4:-45357804 MS.gene85299:intron
CACTGCACAGAGAGCAACAG+TGG 0.628872 2.4:+45358915 None:intergenic
GTGGAGGTGCATGATAATGA+AGG 0.652320 2.4:-45358880 MS.gene85299:CDS
TGCAGTGCTAACTAACGCCG+TGG 0.654951 2.4:-45358899 MS.gene85299:CDS
TATAGCAATTTAGAGAGAGG+TGG 0.656702 2.4:+45355735 None:intergenic
CGACGGTGCAGCGATTCTGG+AGG 0.659267 2.4:-45358941 MS.gene85299:CDS
GTGGTCCAAAATTGATTGTG+AGG 0.672157 2.4:-45357785 MS.gene85299:CDS
CAGGAAATATCTGTTGTCTG+TGG 0.685663 2.4:-45355968 MS.gene85299:CDS
AGTGCTAACTAACGCCGTGG+AGG 0.715440 2.4:-45358896 MS.gene85299:CDS
CAGATAATAAATCACCAGCG+CGG 0.796906 2.4:+45355687 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTAAAAATTAATTAATTG+AGG - chr2.4:45356142-45356161 MS.gene85299:intron 10.0%
!!! TATATCAATTTTTTATCAAT+TGG - chr2.4:45356592-45356611 MS.gene85299:intron 10.0%
!! AAGAAATAAAAAATTACTTG+TGG + chr2.4:45355296-45355315 None:intergenic 15.0%
!! TATAATATAAACCATCAATT+TGG - chr2.4:45356865-45356884 MS.gene85299:intron 15.0%
!!! ACATTTTCTCAAATTATTAT+CGG + chr2.4:45355528-45355547 None:intergenic 15.0%
!! AAAAAAATTCACTAGATGTT+CGG - chr2.4:45358776-45358795 MS.gene85299:CDS 20.0%
!! ACAATTTCATCTAATTTATG+AGG + chr2.4:45357596-45357615 None:intergenic 20.0%
!! AGTATCAAGAGAATTAAAAA+GGG - chr2.4:45356225-45356244 MS.gene85299:CDS 20.0%
!! AGTTTCCTAAATTCATAAAT+GGG + chr2.4:45357526-45357545 None:intergenic 20.0%
!! ATTCTTAATGTATCAAGTTA+GGG - chr2.4:45355644-45355663 MS.gene85299:CDS 20.0%
!! CTAATATTGAATTATTGTTC+AGG - chr2.4:45358000-45358019 MS.gene85299:intron 20.0%
!! CTTCATTATTATACAAGTAA+GGG - chr2.4:45357147-45357166 MS.gene85299:intron 20.0%
!! GAAATTAAGTCAAAAAAATG+TGG + chr2.4:45356952-45356971 None:intergenic 20.0%
!! GATGAATTGAGAATAATTTA+GGG - chr2.4:45355992-45356011 MS.gene85299:intron 20.0%
!! TATTCTTAATGTATCAAGTT+AGG - chr2.4:45355643-45355662 MS.gene85299:CDS 20.0%
!! TGATGAATTGAGAATAATTT+AGG - chr2.4:45355991-45356010 MS.gene85299:intron 20.0%
!! TTCAAGATATATTATTTCCA+AGG + chr2.4:45357397-45357416 None:intergenic 20.0%
!! TTTATTAAATCCTAGGATTA+TGG - chr2.4:45356569-45356588 MS.gene85299:intron 20.0%
!!! AATTTGTATTTGGCTTTTTA+GGG - chr2.4:45357726-45357745 MS.gene85299:CDS 20.0%
!!! ACTAGTTACTTTTCAAAAAT+AGG + chr2.4:45355962-45355981 None:intergenic 20.0%
!!! ATTGATATTCTAGTTTAGTT+AGG - chr2.4:45357859-45357878 MS.gene85299:intron 20.0%
!!! TAAGTTTCTTAATGTTGATT+TGG - chr2.4:45358516-45358535 MS.gene85299:intron 20.0%
!!! TATTTTTGAAAAGTAACTAG+TGG - chr2.4:45355961-45355980 MS.gene85299:CDS 20.0%
!!! TCATTATATGTTTCTAGTTT+AGG - chr2.4:45356020-45356039 MS.gene85299:intron 20.0%
!!! TGGATAGATTTTGTATTAAA+AGG + chr2.4:45357432-45357451 None:intergenic 20.0%
!!! TTATTGGCTTATATATGTTA+TGG - chr2.4:45358931-45358950 MS.gene85299:CDS 20.0%
!!! TTTTTTTTGCATGAAAAAGA+AGG - chr2.4:45356168-45356187 MS.gene85299:CDS 20.0%
! AAAAAATTCACTAGATGTTC+GGG - chr2.4:45358777-45358796 MS.gene85299:CDS 25.0%
! AAAGGAATAATTATATGCTG+TGG - chr2.4:45356461-45356480 MS.gene85299:intron 25.0%
! AAGCTATAAACTATTTCCAT+AGG + chr2.4:45355796-45355815 None:intergenic 25.0%
! AATTGATATACCATAATCCT+AGG + chr2.4:45356582-45356601 None:intergenic 25.0%
! AGAACAAAACTAACCAAAAT+AGG + chr2.4:45356662-45356681 None:intergenic 25.0%
! ATAAATTTGATGCATACCTT+TGG + chr2.4:45356288-45356307 None:intergenic 25.0%
! ATTTATCTTGTGTAATCACA+TGG - chr2.4:45355548-45355567 MS.gene85299:CDS 25.0%
! CAAAATTTAAATGCTGTAAG+TGG - chr2.4:45358563-45358582 MS.gene85299:intron 25.0%
! CAGTTTCCTAAATTCATAAA+TGG + chr2.4:45357527-45357546 None:intergenic 25.0%
! CCTTACTTGTATAATAATGA+AGG + chr2.4:45357149-45357168 None:intergenic 25.0%
! CCTTCATTATTATACAAGTA+AGG - chr2.4:45357146-45357165 MS.gene85299:intron 25.0%
! CGTCATATAGTTGTATAATT+TGG + chr2.4:45356074-45356093 None:intergenic 25.0%
! GAGTATCAAGAGAATTAAAA+AGG - chr2.4:45356224-45356243 MS.gene85299:CDS 25.0%
! GTATCAAGAGAATTAAAAAG+GGG - chr2.4:45356226-45356245 MS.gene85299:CDS 25.0%
! TATCAAGAGAATTAAAAAGG+GGG - chr2.4:45356227-45356246 MS.gene85299:CDS 25.0%
! TCATGATGCTATTAATTAGT+GGG - chr2.4:45356616-45356635 MS.gene85299:intron 25.0%
! TGACTTAATTTCATGAGTTT+AGG - chr2.4:45356960-45356979 MS.gene85299:intron 25.0%
! TTATACATAAGTGCTTATCA+TGG - chr2.4:45355863-45355882 MS.gene85299:CDS 25.0%
! TTCTAGTCTCAATTTGTATT+TGG - chr2.4:45357716-45357735 MS.gene85299:intron 25.0%
! TTTATCTTGTGTAATCACAT+GGG - chr2.4:45355549-45355568 MS.gene85299:CDS 25.0%
!! TATTTGCTACATAATTCGTT+AGG - chr2.4:45355450-45355469 MS.gene85299:CDS 25.0%
!! TATTTTCATAAGATAGCCTA+TGG - chr2.4:45355777-45355796 MS.gene85299:CDS 25.0%
!! TCAATATTAGTTTCACTTCT+GGG + chr2.4:45357990-45358009 None:intergenic 25.0%
!! TGATGATATGCTTAGATTTT+TGG - chr2.4:45356117-45356136 MS.gene85299:intron 25.0%
!! TTCAATATTAGTTTCACTTC+TGG + chr2.4:45357991-45358010 None:intergenic 25.0%
!! TTTTCTCTACCATAATATAC+AGG + chr2.4:45357184-45357203 None:intergenic 25.0%
!!! AGTTGGTTTTATTAAATCCT+AGG - chr2.4:45356562-45356581 MS.gene85299:intron 25.0%
!!! CAATTTGTATTTGGCTTTTT+AGG - chr2.4:45357725-45357744 MS.gene85299:CDS 25.0%
!!! TAAGATACGCTTTTTATTGA+AGG + chr2.4:45355608-45355627 None:intergenic 25.0%
!!! TGTATTAGTTTTTCATGCAT+TGG - chr2.4:45356314-45356333 MS.gene85299:intron 25.0%
!!! TGTCTTACATGATTACTTTT+AGG - chr2.4:45357119-45357138 MS.gene85299:intron 25.0%
!!! TGTTTAGAATTTTGAGTGAA+TGG + chr2.4:45355272-45355291 None:intergenic 25.0%
AAATTAGAGAGTGATATTCG+AGG - chr2.4:45356046-45356065 MS.gene85299:intron 30.0%
AAGTAGAGAAGAAGAAGAAT+TGG + chr2.4:45355222-45355241 None:intergenic 30.0%
AATAAGCTGTATATCCAAAC+AGG - chr2.4:45355910-45355929 MS.gene85299:CDS 30.0%
AATATATGCTACATATCGAG+TGG + chr2.4:45357456-45357475 None:intergenic 30.0%
AATTAGAGAGTGATATTCGA+GGG - chr2.4:45356047-45356066 MS.gene85299:intron 30.0%
AGAGTGGAAATTTAATACCA+GGG + chr2.4:45355937-45355956 None:intergenic 30.0%
ATAAGCTGTATATCCAAACA+GGG - chr2.4:45355911-45355930 MS.gene85299:CDS 30.0%
ATAATTATTCCTTTGCTGCT+TGG + chr2.4:45356455-45356474 None:intergenic 30.0%
CCTTGCATGATTTCAAAAAA+TGG + chr2.4:45358726-45358745 None:intergenic 30.0%
CTAGATGATTTCTATATCAC+TGG - chr2.4:45356683-45356702 MS.gene85299:intron 30.0%
CTCATGATGCTATTAATTAG+TGG - chr2.4:45356615-45356634 MS.gene85299:intron 30.0%
CTTAACATATGGAGGTTTAT+TGG - chr2.4:45358915-45358934 MS.gene85299:CDS 30.0%
GAATAGATGGAATGTTGTAA+TGG - chr2.4:45356506-45356525 MS.gene85299:intron 30.0%
GACTTGAATCCTGTATATTA+TGG - chr2.4:45357172-45357191 MS.gene85299:intron 30.0%
GATTTGACCATTTAACATAC+AGG + chr2.4:45358611-45358630 None:intergenic 30.0%
TAACAAAAAATATGCAGCAC+TGG + chr2.4:45358377-45358396 None:intergenic 30.0%
TAAGTCAAAAAAATGTGGCA+TGG + chr2.4:45356947-45356966 None:intergenic 30.0%
TAATTTCATGAGTTTAGGCT+TGG - chr2.4:45356965-45356984 MS.gene85299:intron 30.0%
TATAACATCTTCGATGGAAT+CGG + chr2.4:45358107-45358126 None:intergenic 30.0%
TCAAATAAGTCAATCCATGT+AGG + chr2.4:45355670-45355689 None:intergenic 30.0%
TCATATAGCAATTTAGAGAG+AGG + chr2.4:45358258-45358277 None:intergenic 30.0%
TCATATCAAAAGCTTTCGAT+TGG + chr2.4:45358348-45358367 None:intergenic 30.0%
TCGAATATCACTCTCTAATT+TGG + chr2.4:45356047-45356066 None:intergenic 30.0%
TTAGGAAACTGTCAACTAAA+AGG - chr2.4:45357536-45357555 MS.gene85299:intron 30.0%
! ATACTCCTCACAATCAATTT+TGG + chr2.4:45356210-45356229 None:intergenic 30.0%
! ATATTACAGAGAGCATATCT+GGG + chr2.4:45357939-45357958 None:intergenic 30.0%
! TAAATTTTGTGTCAGAAGCA+AGG + chr2.4:45358552-45358571 None:intergenic 30.0%
! TGTATCTTTGAGTCCTATTT+TGG - chr2.4:45356646-45356665 MS.gene85299:intron 30.0%
! TTGAGTTTATGTACTGACAT+AGG - chr2.4:45355686-45355705 MS.gene85299:CDS 30.0%
! TTGCATCAGTTGCTAATTTT+TGG - chr2.4:45358443-45358462 MS.gene85299:intron 30.0%
! TTTAGAGCCTGTATGTTAAA+TGG - chr2.4:45358601-45358620 MS.gene85299:CDS 30.0%
! TTTGTTGTATAGTATCTCCT+TGG - chr2.4:45357377-45357396 MS.gene85299:intron 30.0%
! TTTTCAAAAATAGGAGAGAG+TGG + chr2.4:45355953-45355972 None:intergenic 30.0%
! TTTTGCATGAAAAAGAAGGA+CGG - chr2.4:45356172-45356191 MS.gene85299:CDS 30.0%
! TTTTTGAACATGCTTTCTCT+TGG - chr2.4:45355150-45355169 MS.gene85299:intron 30.0%
!! GTAACACATATTTTTCAGCT+TGG - chr2.4:45358883-45358902 MS.gene85299:CDS 30.0%
!! TCAGTTGCTAATTTTTGGAT+AGG - chr2.4:45358448-45358467 MS.gene85299:intron 30.0%
!!! CCATTTTTTGAAATCATGCA+AGG - chr2.4:45358723-45358742 MS.gene85299:CDS 30.0%
!!! TGCTGCATATTTTTTGTTAC+TGG - chr2.4:45358378-45358397 MS.gene85299:intron 30.0%
AAGAATTGGAGAAGGAGAAT+CGG + chr2.4:45355208-45355227 None:intergenic 35.0%
ACCTATGGACATTAATACAG+AGG + chr2.4:45357042-45357061 None:intergenic 35.0%
AGAAGAAGAAGAATTGGAGA+AGG + chr2.4:45355216-45355235 None:intergenic 35.0%
AGTCATTAACCCCATTGTAT+GGG + chr2.4:45357010-45357029 None:intergenic 35.0%
ATAGCAACAGTGTGAATAGA+TGG - chr2.4:45356493-45356512 MS.gene85299:intron 35.0%
ATGCTGTAAGTGGTCATTAA+TGG - chr2.4:45358573-45358592 MS.gene85299:intron 35.0%
ATTACCACTCACCAAATTGA+TGG + chr2.4:45356879-45356898 None:intergenic 35.0%
CAGACATTACTCGTAATGTT+TGG - chr2.4:45358680-45358699 MS.gene85299:intron 35.0%
CATGAATCTGTGAATAGAAC+TGG - chr2.4:45358832-45358851 MS.gene85299:CDS 35.0%
CCAAAATTGAGACAAGTTAC+AGG + chr2.4:45357266-45357285 None:intergenic 35.0%
CCATTGAAAAAGAGATACAG+TGG + chr2.4:45357334-45357353 None:intergenic 35.0%
CTCTGATATAACATCTTCGA+TGG + chr2.4:45358113-45358132 None:intergenic 35.0%
GACAGTGATAAATGAAGTTG+TGG - chr2.4:45357085-45357104 MS.gene85299:intron 35.0%
GAGAGTGGAAATTTAATACC+AGG + chr2.4:45355938-45355957 None:intergenic 35.0%
GATGTGCAAGTAATGACAAA+AGG + chr2.4:45356792-45356811 None:intergenic 35.0%
GGTGTACATTGCTTAACATA+TGG - chr2.4:45358904-45358923 MS.gene85299:CDS 35.0%
GTACATTGCTTAACATATGG+AGG - chr2.4:45358907-45358926 MS.gene85299:CDS 35.0%
TAGTCATTAACCCCATTGTA+TGG + chr2.4:45357011-45357030 None:intergenic 35.0%
TATAGCAATTTAGAGAGAGG+TGG + chr2.4:45358255-45358274 None:intergenic 35.0%
TCAAACAAGATCTGAGAAAG+AGG + chr2.4:45357662-45357681 None:intergenic 35.0%
TCCATGAAATGTTTGACGAA+AGG + chr2.4:45357889-45357908 None:intergenic 35.0%
TCCTAAATTCATAAATGGGC+AGG + chr2.4:45357522-45357541 None:intergenic 35.0%
TCCTCTGTATTAATGTCCAT+AGG - chr2.4:45357038-45357057 MS.gene85299:intron 35.0%
TCCTGCCCATTTATGAATTT+AGG - chr2.4:45357518-45357537 MS.gene85299:intron 35.0%
TCTTGAAATACAGTGACAAC+AGG + chr2.4:45357490-45357509 None:intergenic 35.0%
TGATGTTCATGGTTATGTGT+AGG - chr2.4:45356715-45356734 MS.gene85299:intron 35.0%
TGATTTCTATATCACTGGAG+TGG - chr2.4:45356688-45356707 MS.gene85299:intron 35.0%
TTAACTCTTACCAGAAGTTC+AGG - chr2.4:45358487-45358506 MS.gene85299:intron 35.0%
TTATCACTGTCATTGCTCAT+TGG + chr2.4:45357076-45357095 None:intergenic 35.0%
TTATGATCCACGTATGTGAA+GGG + chr2.4:45357819-45357838 None:intergenic 35.0%
TTGAACCTGAGAAACCAAAT+GGG + chr2.4:45358965-45358984 None:intergenic 35.0%
TTTATGATCCACGTATGTGA+AGG + chr2.4:45357820-45357839 None:intergenic 35.0%
TTTGAACCTGAGAAACCAAA+TGG + chr2.4:45358966-45358985 None:intergenic 35.0%
TTTGCATGAAAAAGAAGGAC+GGG - chr2.4:45356173-45356192 MS.gene85299:CDS 35.0%
! ATATCAGAGTACCTTTCAGT+TGG - chr2.4:45358124-45358143 MS.gene85299:intron 35.0%
! CAGATAGATTGTGGTGTTTT+TGG - chr2.4:45355379-45355398 MS.gene85299:intron 35.0%
! CATATTACAGAGAGCATATC+TGG + chr2.4:45357940-45357959 None:intergenic 35.0%
! GTCTCTGAAGAACTTGTAAT+TGG + chr2.4:45358065-45358084 None:intergenic 35.0%
! TAAACCATCAATTTGGTGAG+TGG - chr2.4:45356872-45356891 MS.gene85299:intron 35.0%
! TATTACAGAGAGCATATCTG+GGG + chr2.4:45357938-45357957 None:intergenic 35.0%
! TGTGTTTTGCAGATAGATTG+TGG - chr2.4:45355370-45355389 MS.gene85299:intron 35.0%
! TTACTAGCTGAAGTGAGTTT+TGG - chr2.4:45356539-45356558 MS.gene85299:intron 35.0%
!! CCTGTAACTTGTCTCAATTT+TGG - chr2.4:45357263-45357282 MS.gene85299:intron 35.0%
!! TTTTTAGGGAACGAGACAAT+CGG - chr2.4:45357740-45357759 MS.gene85299:CDS 35.0%
!!! CCACTGTATCTCTTTTTCAA+TGG - chr2.4:45357331-45357350 MS.gene85299:intron 35.0%
!!! GCATATTTTTTGTTACTGGC+AGG - chr2.4:45358382-45358401 MS.gene85299:intron 35.0%
AAAAATGGCAGTCATAGACG+AGG + chr2.4:45358711-45358730 None:intergenic 40.0%
AACAAGATCTGAGAAAGAGG+AGG + chr2.4:45357659-45357678 None:intergenic 40.0%
AATTTAGAGAGAGGTGGTGA+AGG + chr2.4:45358249-45358268 None:intergenic 40.0%
ACGTTGTGCAAATTCACTAC+TGG + chr2.4:45355407-45355426 None:intergenic 40.0%
ATGCAGAATCACCAACTGAA+AGG + chr2.4:45358138-45358157 None:intergenic 40.0%
ATGGTTATGTGTAGGAGCAA+TGG - chr2.4:45356723-45356742 MS.gene85299:intron 40.0%
ATGTCCATAGGTTGTAGCAA+TGG - chr2.4:45357050-45357069 MS.gene85299:intron 40.0%
ATTACCCAGCCGAAATTGAT+AGG + chr2.4:45358751-45358770 None:intergenic 40.0%
ATTCATAAATGGGCAGGAAG+AGG + chr2.4:45357516-45357535 None:intergenic 40.0%
ATTGTGTCTGTGTCAGACAT+CGG - chr2.4:45355577-45355596 MS.gene85299:CDS 40.0%
ATTTAGAGAGAGGTGGTGAA+GGG + chr2.4:45358248-45358267 None:intergenic 40.0%
CAGATAATAAATCACCAGCG+CGG + chr2.4:45358303-45358322 None:intergenic 40.0%
CAGGAAATATCTGTTGTCTG+TGG - chr2.4:45358019-45358038 MS.gene85299:intron 40.0%
CGGAACTATGGTCTAAGTAT+CGG - chr2.4:45357760-45357779 MS.gene85299:CDS 40.0%
CTGAGAAACCAAATGGGAAT+GGG + chr2.4:45358959-45358978 None:intergenic 40.0%
GAGAATTAAAAAGGGGGAAG+AGG - chr2.4:45356233-45356252 MS.gene85299:CDS 40.0%
GATAAACGAACAAGTACGAG+TGG - chr2.4:45356349-45356368 MS.gene85299:intron 40.0%
GATACAGTGGTTTCTCAGTA+TGG + chr2.4:45357321-45357340 None:intergenic 40.0%
GATATAGTACCAAGCAGCAA+AGG - chr2.4:45356443-45356462 MS.gene85299:intron 40.0%
GCCTTTCGTCAAACATTTCA+TGG - chr2.4:45357885-45357904 MS.gene85299:intron 40.0%
GTCATTACTTGCACATCTTG+TGG - chr2.4:45356795-45356814 MS.gene85299:intron 40.0%
GTGGTCCAAAATTGATTGTG+AGG - chr2.4:45356202-45356221 MS.gene85299:CDS 40.0%
TATGCTACATATCGAGTGGA+TGG + chr2.4:45357452-45357471 None:intergenic 40.0%
TATGTGTAGGAGCAATGGTT+TGG - chr2.4:45356728-45356747 MS.gene85299:intron 40.0%
TGAAATGTTTGACGAAAGGC+TGG + chr2.4:45357885-45357904 None:intergenic 40.0%
TGAGAAACCAAATGGGAATG+GGG + chr2.4:45358958-45358977 None:intergenic 40.0%
TGCAAGGACCCTATCAATTT+CGG - chr2.4:45358739-45358758 MS.gene85299:CDS 40.0%
TGTCAGAAGCAAGGTTTGAA+AGG + chr2.4:45358543-45358562 None:intergenic 40.0%
TTACCCAGCCGAAATTGATA+GGG + chr2.4:45358750-45358769 None:intergenic 40.0%
TTACTTGCACATCTTGTGGT+TGG - chr2.4:45356799-45356818 MS.gene85299:intron 40.0%
TTCATTATCATGCACCTCCA+CGG + chr2.4:45355108-45355127 None:intergenic 40.0%
! CGAAAGCTTTTGATATGAGC+AGG - chr2.4:45358350-45358369 MS.gene85299:intron 40.0%
!! ACCGTTTTGCTTTTCTACTG+CGG + chr2.4:45355018-45355037 None:intergenic 40.0%
!! GCTAATTTTTGGATAGGAGC+TGG - chr2.4:45358454-45358473 MS.gene85299:intron 40.0%
!!! TAGGAGCAATGGTTTGGTTT+TGG - chr2.4:45356734-45356753 MS.gene85299:intron 40.0%
AACCCCATTGTATGGGAACA+GGG + chr2.4:45357003-45357022 None:intergenic 45.0%
ACAGTGGTTTCTCAGTATGG+TGG + chr2.4:45357318-45357337 None:intergenic 45.0%
ATAATGAAGGATGCGCACTC+GGG - chr2.4:45355120-45355139 MS.gene85299:intron 45.0%
CCTGAGAAACCAAATGGGAA+TGG + chr2.4:45358960-45358979 None:intergenic 45.0%
CGGGCTCATGATATCAAAAC+TGG - chr2.4:45358796-45358815 MS.gene85299:CDS 45.0%
CTCGAGAAGATTCTCATCGA+AGG - chr2.4:45358172-45358191 MS.gene85299:intron 45.0%
CTCTTACCAGAAGTTCAGGA+TGG - chr2.4:45358491-45358510 MS.gene85299:intron 45.0%
CTTACTCCATCCTGAACTTC+TGG + chr2.4:45358500-45358519 None:intergenic 45.0%
GAACGAGACAATCGGAACTA+TGG - chr2.4:45357748-45357767 MS.gene85299:CDS 45.0%
GAATGGAGCTATGCGAAGTT+TGG + chr2.4:45355255-45355274 None:intergenic 45.0%
GAGTGGAAGAGGAGAAAGTA+TGG - chr2.4:45356366-45356385 MS.gene85299:intron 45.0%
GATAATGAAGGATGCGCACT+CGG - chr2.4:45355119-45355138 MS.gene85299:intron 45.0%
GCCGCAGTAGAAAAGCAAAA+CGG - chr2.4:45355014-45355033 MS.gene85299:intron 45.0%
GTATCAAGTTAGGGCCTACA+TGG - chr2.4:45355653-45355672 MS.gene85299:CDS 45.0%
TAACCCCATTGTATGGGAAC+AGG + chr2.4:45357004-45357023 None:intergenic 45.0%
TGAGTTTAGGCTTGGAGTCT+TGG - chr2.4:45356973-45356992 MS.gene85299:intron 45.0%
TTATGGTCCCCATTCCCATT+TGG - chr2.4:45358948-45358967 MS.gene85299:CDS 45.0%
TTGGCCATTGCTACAACCTA+TGG + chr2.4:45357057-45357076 None:intergenic 45.0%
TTGTGTCTGTGTCAGACATC+GGG - chr2.4:45355578-45355597 MS.gene85299:CDS 45.0%
TTTAATACCAGGGCCCTGTT+TGG + chr2.4:45355927-45355946 None:intergenic 45.0%
TTTAGAGAGAGGTGGTGAAG+GGG + chr2.4:45358247-45358266 None:intergenic 45.0%
! CCATTCCCATTTGGTTTCTC+AGG - chr2.4:45358957-45358976 MS.gene85299:CDS 45.0%
! CGCGCTGGTGATTTATTATC+TGG - chr2.4:45358301-45358320 MS.gene85299:intron 45.0%
! GTGGAGGTGCATGATAATGA+AGG - chr2.4:45355107-45355126 MS.gene85299:intron 45.0%
!!! GCTGAAGTGAGTTTTGGAGT+TGG - chr2.4:45356545-45356564 MS.gene85299:intron 45.0%
AAAGAAGGACGGGTGATCTG+TGG - chr2.4:45356183-45356202 MS.gene85299:CDS 50.0%
ACACCCCTGTTCCCATACAA+TGG - chr2.4:45356996-45357015 MS.gene85299:intron 50.0%
ACCCCATTGTATGGGAACAG+GGG + chr2.4:45357002-45357021 None:intergenic 50.0%
ACCCCTGTTCCCATACAATG+GGG - chr2.4:45356998-45357017 MS.gene85299:intron 50.0%
AGGACCCTATCAATTTCGGC+TGG - chr2.4:45358743-45358762 MS.gene85299:CDS 50.0%
CAAAACGGTTGCAGCAACGA+CGG - chr2.4:45355029-45355048 MS.gene85299:intron 50.0%
CACCCCTGTTCCCATACAAT+GGG - chr2.4:45356997-45357016 MS.gene85299:intron 50.0%
CGAACAAGTACGAGTGGAAG+AGG - chr2.4:45356355-45356374 MS.gene85299:intron 50.0%
GAGAATCGGTAGCACGCATT+AGG + chr2.4:45355194-45355213 None:intergenic 50.0%
GGACCCTATCAATTTCGGCT+GGG - chr2.4:45358744-45358763 MS.gene85299:CDS 50.0%
GGAGTGGATGCTGATGTTCA+TGG - chr2.4:45356704-45356723 MS.gene85299:intron 50.0%
GTTGTCTGTGGAGATTCGTC+TGG - chr2.4:45358031-45358050 MS.gene85299:intron 50.0%
TACTGACCTCTTGCAGCCAA+AGG - chr2.4:45356269-45356288 MS.gene85299:intron 50.0%
TCAGCATATAAAGTCCGCGC+TGG - chr2.4:45358286-45358305 MS.gene85299:intron 50.0%
TGCATACCTTTGGCTGCAAG+AGG + chr2.4:45356278-45356297 None:intergenic 50.0%
TGTATATCCAAACAGGGCCC+TGG - chr2.4:45355917-45355936 MS.gene85299:CDS 50.0%
AGGGCAGAGAGCTACAACGT+TGG + chr2.4:45357800-45357819 None:intergenic 55.0%
AGTGCTAACTAACGCCGTGG+AGG - chr2.4:45355091-45355110 MS.gene85299:intron 55.0%
CACTGCACAGAGAGCAACAG+TGG + chr2.4:45355075-45355094 None:intergenic 55.0%
CTCTCTGCCCTTCACATACG+TGG - chr2.4:45357809-45357828 MS.gene85299:intron 55.0%
GGTGTAGACGAGCAGCTAGA+AGG - chr2.4:45358193-45358212 MS.gene85299:intron 55.0%
TGCAGTGCTAACTAACGCCG+TGG - chr2.4:45355088-45355107 MS.gene85299:intron 55.0%
AGGATGCGCACTCGGGATTG+CGG - chr2.4:45355127-45355146 MS.gene85299:intron 60.0%
CAACGACGGTGCAGCGATTC+TGG - chr2.4:45355043-45355062 MS.gene85299:intron 60.0%
CGACGGTGCAGCGATTCTGG+AGG - chr2.4:45355046-45355065 MS.gene85299:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 45355008 45359001 45355008 ID=MS.gene85299
chr2.4 mRNA 45355008 45359001 45355008 ID=MS.gene85299.t1;Parent=MS.gene85299
chr2.4 exon 45358711 45359001 45358711 ID=MS.gene85299.t1.exon1;Parent=MS.gene85299.t1
chr2.4 CDS 45358711 45359001 45358711 ID=cds.MS.gene85299.t1;Parent=MS.gene85299.t1
chr2.4 exon 45358585 45358627 45358585 ID=MS.gene85299.t1.exon2;Parent=MS.gene85299.t1
chr2.4 CDS 45358585 45358627 45358585 ID=cds.MS.gene85299.t1;Parent=MS.gene85299.t1
chr2.4 exon 45357719 45357822 45357719 ID=MS.gene85299.t1.exon3;Parent=MS.gene85299.t1
chr2.4 CDS 45357719 45357822 45357719 ID=cds.MS.gene85299.t1;Parent=MS.gene85299.t1
chr2.4 exon 45356164 45356262 45356164 ID=MS.gene85299.t1.exon4;Parent=MS.gene85299.t1
chr2.4 CDS 45356164 45356262 45356164 ID=cds.MS.gene85299.t1;Parent=MS.gene85299.t1
chr2.4 exon 45355432 45355987 45355432 ID=MS.gene85299.t1.exon5;Parent=MS.gene85299.t1
chr2.4 CDS 45355432 45355987 45355432 ID=cds.MS.gene85299.t1;Parent=MS.gene85299.t1
chr2.4 exon 45355192 45355326 45355192 ID=MS.gene85299.t1.exon6;Parent=MS.gene85299.t1
chr2.4 CDS 45355192 45355326 45355192 ID=cds.MS.gene85299.t1;Parent=MS.gene85299.t1
chr2.4 exon 45355008 45355030 45355008 ID=MS.gene85299.t1.exon7;Parent=MS.gene85299.t1
chr2.4 CDS 45355008 45355030 45355008 ID=cds.MS.gene85299.t1;Parent=MS.gene85299.t1
Gene Sequence

>MS.gene85299

ATGGCTGCCGCAGTAGAAAAGCAAAACGGTTGCAGCAACGACGGTGCAGCGATTCTGGAGGATGCCACTGTTGCTCTCTGTGCAGTGCTAACTAACGCCGTGGAGGTGCATGATAATGAAGGATGCGCACTCGGGATTGCGGTTTTTGAACATGCTTTCTCTTGGATTAATCATAGTTGTTCTCCTAATGCGTGCTACCGATTCTCCTTCTCCAATTCTTCTTCTTCTCTACTTTCTCATGAATCCAAACTTCGCATAGCTCCATTCACTCAAAATTCTAAACAACCACAAGTAATTTTTTATTTCTTTGCTACTTTTGTGCAGTTTTGCTTCTGCTATTTTCTAACATGATGTGAATTGTTTGTGTTTTGCAGATAGATTGTGGTGTTTTTGGTACCAGTAGTGAATTTGCACAACGTTTGTTTCTTTTTTATTACATTTATATTTGCTACATAATTCGTTAGGACTTTATATTAAACAATTCATATTTGCTAAGCACGACACTCACACGTTCACACCGATAATAATTTGAGAAAATGTATTTATCTTGTGTAATCACATGGGTTAGTATTGTGTCTGTGTCAGACATCGGGAACACCTTCAATAAAAAGCGTATCTTACAGAGTTTCATAAGCTATTCTTAATGTATCAAGTTAGGGCCTACATGGATTGACTTATTTGAGTTTATGTACTGACATAGGTTTTATGAGACTGTTTGCGAGAACTTATAAACACAGCTTATGACATTCTTCATAAGCTATTTTGAGCTTATTTTCATAAGATAGCCTATGGAAATAGTTTATAGCTTACATGAAAAAAATTTAACTTTTATTTATCTTTTGCTATAAACATAGCTTATACATAAGTGCTTATCATGGTATGTGCTTGTGCTATAAGCTGCAAATAAGCTGTATATCCAAACAGGGCCCTGGTATTAAATTTCCACTCTCTCCTATTTTTGAAAAGTAACTAGTGGACTAACTTGATGAATTGAGAATAATTTAGGGACTATTCATTATATGTTTCTAGTTTAGGACCAAATTAGAGAGTGATATTCGAGGGACCAAATTATACAACTATATGACGTTCTTACACAACTTTGTTCTATCTGATGATATGCTTAGATTTTTGGTTTTGTAAAAATTAATTAATTGAGGTGATTTTTTTTGCATGAAAAAGAAGGACGGGTGATCTGTGGTCCAAAATTGATTGTGAGGAGTATCAAGAGAATTAAAAAGGGGGAAGAGGTCACTGTAGCATATACTGACCTCTTGCAGCCAAAGGTATGCATCAAATTTATGTTATTTGTATTAGTTTTTCATGCATTGGAACTGAGAGAGTGATAAACGAACAAGTACGAGTGGAAGAGGAGAAAGTATGGAAAATGCTAGATGAATTGAATTTATACATTGAATTTACAATTTTTTAACATTCTGATATAGTACCAAGCAGCAAAGGAATAATTATATGCTGTGGTAAACAGAAATAGCAACAGTGTGAATAGATGGAATGTTGTAATGGTATATCAGATTTACTAGCTGAAGTGAGTTTTGGAGTTGGTTTTATTAAATCCTAGGATTATGGTATATCAATTTTTTATCAATTGGCTCATGATGCTATTAATTAGTGGGATGTATGTGTATCTTTGAGTCCTATTTTGGTTAGTTTTGTTCTGCTAGATGATTTCTATATCACTGGAGTGGATGCTGATGTTCATGGTTATGTGTAGGAGCAATGGTTTGGTTTTGGTATTTGCTGCATTTATACAGTTTTTGTATAAACCTTTTGTCATTACTTGCACATCTTGTGGTTGGATTATGCTCATAAATAAATTCATTTGTCTCTTCAAAAATAAAATATAATATAAACCATCAATTTGGTGAGTGGTAATAACAACATATTATGTTCTTATGATATATGCAGTGTATATAAATGTCCATGCCACATTTTTTTGACTTAATTTCATGAGTTTAGGCTTGGAGTCTTGGACACCCCTGTTCCCATACAATGGGGTTAATGACTATGTTATGTCCTCTGTATTAATGTCCATAGGTTGTAGCAATGGCCAATGAGCAATGACAGTGATAAATGAAGTTGTGGTTTTGTTGCTTTGTCTTACATGATTACTTTTAGGTTCGCCTTCATTATTATACAAGTAAGGGCTGACTTGAATCCTGTATATTATGGTAGAGAAAATTAATGATGCTAGTATCTTACAGTGATGTATGAGTCACAATTCGTGTGTTCATGACTATCCTGTAACTTGTCTCAATTTTGGACATGAAAACATTTTTTTAATATATTTGTCCACCATACTGAGAAACCACTGTATCTCTTTTTCAATGGATATTAGTTTTTGTTTATCTTTCTTTGTTGTATAGTATCTCCTTGGAAATAATATATCTTGAAAAGCAATAATTTCCTTTTAATACAAAATCTATCCATCCACTCGATATGTAGCATATATTACATATAATTTCCTGTTGTCACTGTATTTCAAGACATCCTCTTCCTGCCCATTTATGAATTTAGGAAACTGTCAACTAAAAGGATAGTTATCTGCTAAATTATAAACATAAATGTATCCTCATAAATTAGATGAAATTGTACTCTTTTTTCTCTCACAAAAGTTTGTCTCATGAATTTTTCCTCCTCTTTCTCAGATCTTGTTTGAGTTCTTTCATTTCTTTATTGATATTTTATTAATCTTCTAGTCTCAATTTGTATTTGGCTTTTTAGGGAACGAGACAATCGGAACTATGGTCTAAGTATCGGTTTATTTGTTGCTGCCAACGTTGTAGCTCTCTGCCCTTCACATACGTGGATCATAAATTGCAAGTAAGACTTTGAAATTGATATTCTAGTTTAGTTAGGCCAGCCTTTCGTCAAACATTTCATGGATGTTGTCAAACTAACAGATTTGTTTGCCCCAGATATGCTCTCTGTAATATGAATTTTCTGATAAATATTTGTGACTCGCCCAGAAGTGAAACTAATATTGAATTATTGTTCAGGAAATATCTGTTGTCTGTGGAGATTCGTCTGGTTTACGTTCCAATTACAAGTTCTTCAGAGACATGACTGATAGAAGATTGACCGATTCCATCGAAGATGTTATATCAGAGTACCTTTCAGTTGGTGATTCTGCATCTTGTTGTGAGAAGCTCGAGAAGATTCTCATCGAAGGTGTAGACGAGCAGCTAGAAGGAAAAGCACATTCTCAGTTCACGCTGCATCCCCTTCACCACCTCTCTCTAAATTGCTATATGACACTAGCTTCAGCATATAAAGTCCGCGCTGGTGATTTATTATCTGGAGATTCTGAAATTGATTTTAACCAATCGAAAGCTTTTGATATGAGCAGGACCAGTGCTGCATATTTTTTGTTACTGGCAGGTGCAGCTCATCATCTTTTCAATTCTGAATCATCACTTATTGCATCAGTTGCTAATTTTTGGATAGGAGCTGGTGAATCTTTGTTAACTCTTACCAGAAGTTCAGGATGGAGTAAGTTTCTTAATGTTGATTTGGTCCTTTCAAACCTTGCTTCTGACACAAAATTTAAATGCTGTAAGTGGTCATTAATGGATACTTTTAGAGCCTGTATGTTAAATGGTCAAATCAATTGTGAAGATTTTGAGAATGTATCAAATGAGTTCATTCGTTCTGTCTCAGACATTACTCGTAATGTTTGGAGTTTCCTCGTCTATGACTGCCATTTTTTGAAATCATGCAAGGACCCTATCAATTTCGGCTGGGTAATGTCTGAAAAAAATTCACTAGATGTTCGGGCTCATGATATCAAAACTGGTATGTGTTACACACATGAATCTGTGAATAGAACTGGTTTTCGTGAAGAACAAGATTACAATGCAGTAACACATATTTTTCAGCTTGGTGTACATTGCTTAACATATGGAGGTTTATTGGCTTATATATGTTATGGTCCCCATTCCCATTTGGTTTCTCAGGTTCAAAATATTCTAGATCATAA
Protein sequence

>MS.gene85299.t1

MAAAVEKQNGCSNDGAAILEDATVALCAVLTNAVEVHDNEGCALGIAVFEHAFSWINHSCSPNACYRFSFSNSSSSLLSHESKLRIAPFTQNSKQPQIDCGVFGTSSEFAQQGRVICGPKLIVRSIKRIKKGEEVTVAYTDLLQPKGTRQSELWSKYRFICCCQRCSSLPFTYVDHKLQEISVVCGDSSGLRSNYKFFRDMTDRRLTDSIEDVISEYLSVGDSASCCEKLEKILIEGVDEQLEGKAHSQFTLHPLHHLSLNCYMTLASAYKVRAGDLLSGDSEIDFNQSKAFDMSRTSAAYFLLLAGAAHHLFNSESSLIASVANFWIGAGESLLTLTRSSGWSKFLNVDLVLSNLASDTKFKCYITRNVWSFLVYDCHFLKSCKDPINFGWVMSEKNSLDVRAHDIKTGSKYSRS