AlfalfaGEDB | MS.gene85467

M. sativa cultivar XinJiangDaYe / MS.gene85467

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043565 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03106.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85467.t1	RHN47048.1	86.4	162	22	0	1	162	1	162	7.90E-71	276.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85467.t1	Q9FFS3	63.2	57	13	1	105	161	70	118	3.2e-12	73.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85467.t1	G7L6N9	87.0	161	21	0	1	161	1	161	5.7e-71	276.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene85467.t1	TF	WRKY

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85467.t1	MTR_7g079010	95.652	161	7	0	1	161	1	161	2.42e-113	321
MS.gene85467.t1	MTR_8g007270	65.116	43	15	0	119	161	132	174	1.42e-13	66.2
MS.gene85467.t1	MTR_7g028415	84.848	33	5	0	129	161	81	113	1.69e-13	65.1
MS.gene85467.t1	MTR_7g028710	84.848	33	5	0	129	161	81	113	1.69e-13	65.1
MS.gene85467.t1	MTR_3g095080	68.421	38	12	0	127	164	154	191	2.16e-13	65.1
MS.gene85467.t1	MTR_5g029850	69.231	39	12	0	119	157	29	67	1.36e-12	60.5
MS.gene85467.t1	MTR_5g043880	58.929	56	14	1	106	161	98	144	1.82e-12	63.9
MS.gene85467.t1	MTR_4g094588	80.000	35	7	0	127	161	164	198	1.96e-12	64.3
MS.gene85467.t1	MTR_2g033820	80.000	35	7	0	127	161	189	223	2.00e-12	64.3
MS.gene85467.t1	MTR_1g086790	70.000	40	12	0	122	161	139	178	4.03e-12	63.2
MS.gene85467.t1	MTR_5g091390	80.000	35	7	0	127	161	143	177	6.05e-12	62.8
MS.gene85467.t1	MTR_8g092140	69.444	36	10	1	127	161	145	180	1.76e-11	60.8
MS.gene85467.t1	MTR_7g109800	50.000	62	22	1	100	161	113	165	1.92e-11	61.2
MS.gene85467.t1	MTR_3g095040	58.000	50	20	1	113	161	89	138	2.34e-11	60.1
MS.gene85467.t1	MTR_3g095080	68.571	35	11	0	127	161	154	188	2.40e-11	60.5
MS.gene85467.t1	MTR_3g095080	68.571	35	11	0	127	161	130	164	2.80e-11	60.1
MS.gene85467.t1	MTR_8g096730	48.387	62	29	1	100	161	32	90	3.91e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85467.t1	AT5G41570	63.158	57	13	1	105	161	70	118	3.58e-17	75.1
MS.gene85467.t1	AT2G46130	69.565	46	14	0	122	167	11	56	3.94e-17	72.8
MS.gene85467.t1	AT2G46130	75.000	40	10	0	122	161	11	50	6.63e-15	67.4
MS.gene85467.t1	AT1G64000	65.909	44	15	0	118	161	91	134	7.18e-15	69.3
MS.gene85467.t1	AT5G13080	85.294	34	5	0	128	161	54	87	1.55e-14	67.4
MS.gene85467.t1	AT2G03340	38.947	95	54	1	71	161	341	435	2.16e-13	67.4
MS.gene85467.t1	AT1G69310	44.186	86	42	2	82	167	94	173	2.18e-13	66.6
MS.gene85467.t1	AT2G44745	60.465	43	17	0	119	161	123	165	4.12e-13	65.1
MS.gene85467.t1	AT3G01970	82.353	34	6	0	128	161	52	85	4.46e-13	63.5
MS.gene85467.t1	AT1G69310	46.250	80	37	2	82	161	94	167	7.36e-13	65.1
MS.gene85467.t1	AT1G69310	46.250	80	37	2	82	161	94	167	7.36e-13	65.1
MS.gene85467.t1	AT1G69310	46.250	80	37	2	82	161	94	167	7.36e-13	65.1
MS.gene85467.t1	AT4G39410	52.083	48	23	0	114	161	196	243	2.25e-12	63.9
MS.gene85467.t1	AT5G46350	34.899	149	71	4	28	161	66	203	8.82e-12	62.4
MS.gene85467.t1	AT5G49520	82.353	34	6	0	128	161	208	241	1.18e-11	62.4
MS.gene85467.t1	AT4G18170	62.500	48	14	1	118	161	145	192	1.35e-11	61.6
MS.gene85467.t1	AT2G38470	50.746	67	25	1	103	161	316	382	9.26e-11	59.7

Find 43 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACTTCATCTTCTTCTAATT+TGG	0.109939	7.2:-26459834	MS.gene85467:CDS
AAGAAGTGTTGATGATATTT+TGG	0.221201	7.2:-26459681	MS.gene85467:CDS
ACAACAATAACTTGCTTGTT+GGG	0.273574	7.2:-26459898	MS.gene85467:CDS
ATTAATGGTGGTGGTGGGTT+TGG	0.274937	7.2:+26460061	None:intergenic
GTGGTGGGTTTGGTGGGTCT+TGG	0.304953	7.2:+26460071	None:intergenic
GGCGATGGGATATTCATTAA+TGG	0.315542	7.2:+26460046	None:intergenic
GGTTATCGTTGGAGGAAATA+TGG	0.317694	7.2:-26459653	MS.gene85467:CDS
AACAACAATAACTTGCTTGT+TGG	0.348151	7.2:-26459899	MS.gene85467:CDS
GAAACAAGTTAGAGGAGAAA+AGG	0.356863	7.2:-26459760	MS.gene85467:CDS
TTAGAGGAGAAAAGGGTTAG+AGG	0.362096	7.2:-26459752	MS.gene85467:CDS
TTCTTTACAAAATTACCCTT+TGG	0.380075	7.2:-26459978	MS.gene85467:CDS
AGAAAAGGGAAACAAGTTAG+AGG	0.399768	7.2:-26459768	MS.gene85467:CDS
TCAACTACATGAAGAAGAAA+AGG	0.419090	7.2:-26459783	MS.gene85467:CDS
AAACAAGTTAGAGGAGAAAA+GGG	0.432866	7.2:-26459759	MS.gene85467:CDS
GAATCACAAGATCAAGGTCT+TGG	0.450978	7.2:-26459956	MS.gene85467:CDS
ATGGTGGTGGTGGGTTTGGT+GGG	0.457794	7.2:+26460065	None:intergenic
TGGATGATGGTTATCGTTGG+AGG	0.458848	7.2:-26459661	MS.gene85467:CDS
TAAAGAAGGCGTAAAGAAGA+AGG	0.461538	7.2:+26459994	None:intergenic
ATATTCATTAATGGTGGTGG+TGG	0.463366	7.2:+26460055	None:intergenic
AATGGTGGTGGTGGGTTTGG+TGG	0.469202	7.2:+26460064	None:intergenic
GTAATTCAAAATAATGAAAG+TGG	0.470864	7.2:-26459812	MS.gene85467:CDS
AGGAGAAAAGGGTTAGAGGT+GGG	0.487742	7.2:-26459748	MS.gene85467:CDS
TATTCATTAATGGTGGTGGT+GGG	0.497156	7.2:+26460056	None:intergenic
CTTGCTTGTTGGGGATGCTA+AGG	0.508570	7.2:-26459888	MS.gene85467:CDS
AAAGAAGGCGTAAAGAAGAA+GGG	0.509237	7.2:+26459995	None:intergenic
CTTGATCTTGTGATTCCAAA+GGG	0.509438	7.2:+26459963	None:intergenic
ACATACATGTAGTATATACC+TGG	0.511478	7.2:+26459601	None:intergenic
CAACTACATGAAGAAGAAAA+GGG	0.514940	7.2:-26459782	MS.gene85467:CDS
CCTTTGGAATCACAAGATCA+AGG	0.519006	7.2:-26459962	MS.gene85467:CDS
GAGGAGAAAAGGGTTAGAGG+TGG	0.538687	7.2:-26459749	MS.gene85467:CDS
CAACAATAACTTGCTTGTTG+GGG	0.541537	7.2:-26459897	MS.gene85467:CDS
AAGAAGGCGTAAAGAAGAAG+GGG	0.548737	7.2:+26459996	None:intergenic
GGGATATTCATTAATGGTGG+TGG	0.555899	7.2:+26460052	None:intergenic
CCTTGATCTTGTGATTCCAA+AGG	0.562491	7.2:+26459962	None:intergenic
CTTGTCTGAAATGCAAACCT+TGG	0.566555	7.2:+26459701	None:intergenic
TCAAGAACAGCAAGTATCCC+AGG	0.569780	7.2:-26459619	MS.gene85467:CDS
AGGTCTTGGTGATATTGAGT+GGG	0.577572	7.2:-26459942	MS.gene85467:CDS
AAGGTCTTGGTGATATTGAG+TGG	0.598654	7.2:-26459943	MS.gene85467:CDS
TAATTCAAAATAATGAAAGT+GGG	0.605922	7.2:-26459811	MS.gene85467:CDS
AAAAGACAACAAAAGTGCCA+AGG	0.641958	7.2:-26459718	MS.gene85467:CDS
GATGGGATATTCATTAATGG+TGG	0.659675	7.2:+26460049	None:intergenic
CATACATGTAGTATATACCT+GGG	0.661629	7.2:+26459602	None:intergenic
GGTCTTGGTGATATTGAGTG+GGG	0.698935	7.2:-26459941	MS.gene85467:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAATTCAAAATAATGAAAGT+GGG	-	chr7.2:26459863-26459882	MS.gene85467:CDS	15.0%
!!	GTAATTCAAAATAATGAAAG+TGG	-	chr7.2:26459862-26459881	MS.gene85467:CDS	20.0%
!	TTCTTTACAAAATTACCCTT+TGG	-	chr7.2:26459696-26459715	MS.gene85467:CDS	25.0%
!!	AAAGGGTAATTTTGTAAAGA+AGG	+	chr7.2:26459697-26459716	None:intergenic	25.0%
!!!	AAGAAGTGTTGATGATATTT+TGG	-	chr7.2:26459993-26460012	MS.gene85467:CDS	25.0%
	AAACAAGTTAGAGGAGAAAA+GGG	-	chr7.2:26459915-26459934	MS.gene85467:CDS	30.0%
	ACATACATGTAGTATATACC+TGG	+	chr7.2:26460076-26460095	None:intergenic	30.0%
	CAACTACATGAAGAAGAAAA+GGG	-	chr7.2:26459892-26459911	MS.gene85467:CDS	30.0%
	CACTTCATCTTCTTCTAATT+TGG	-	chr7.2:26459840-26459859	MS.gene85467:CDS	30.0%
	CATACATGTAGTATATACCT+GGG	+	chr7.2:26460075-26460094	None:intergenic	30.0%
	TCAACTACATGAAGAAGAAA+AGG	-	chr7.2:26459891-26459910	MS.gene85467:CDS	30.0%
!!	AACAACAATAACTTGCTTGT+TGG	-	chr7.2:26459775-26459794	MS.gene85467:CDS	30.0%
!!	ACAACAATAACTTGCTTGTT+GGG	-	chr7.2:26459776-26459795	MS.gene85467:CDS	30.0%
!!!	GTTGATGATATTTTGGATGA+TGG	-	chr7.2:26460000-26460019	MS.gene85467:CDS	30.0%
	AGAAAAGGGAAACAAGTTAG+AGG	-	chr7.2:26459906-26459925	MS.gene85467:CDS	35.0%
	CTTGATCTTGTGATTCCAAA+GGG	+	chr7.2:26459714-26459733	None:intergenic	35.0%
	GAAACAAGTTAGAGGAGAAA+AGG	-	chr7.2:26459914-26459933	MS.gene85467:CDS	35.0%
	GATGGGATATTCATTAATGG+TGG	+	chr7.2:26459628-26459647	None:intergenic	35.0%
!	AAAGAAGGCGTAAAGAAGAA+GGG	+	chr7.2:26459682-26459701	None:intergenic	35.0%
!	TAAAGAAGGCGTAAAGAAGA+AGG	+	chr7.2:26459683-26459702	None:intergenic	35.0%
!!	AAAAGACAACAAAAGTGCCA+AGG	-	chr7.2:26459956-26459975	MS.gene85467:CDS	35.0%
!!	ATATTCATTAATGGTGGTGG+TGG	+	chr7.2:26459622-26459641	None:intergenic	35.0%
!!	CAACAATAACTTGCTTGTTG+GGG	-	chr7.2:26459777-26459796	MS.gene85467:CDS	35.0%
!!	TATTCATTAATGGTGGTGGT+GGG	+	chr7.2:26459621-26459640	None:intergenic	35.0%
!!	TTTTGGATGATGGTTATCGT+TGG	-	chr7.2:26460010-26460029	MS.gene85467:CDS	35.0%
!!!	GTGTTGTTGTTTTGAGTTTG+TGG	+	chr7.2:26459652-26459671	None:intergenic	35.0%
	CCTTGATCTTGTGATTCCAA+AGG	+	chr7.2:26459715-26459734	None:intergenic	40.0%
	GAATCACAAGATCAAGGTCT+TGG	-	chr7.2:26459718-26459737	MS.gene85467:CDS	40.0%
	GGCGATGGGATATTCATTAA+TGG	+	chr7.2:26459631-26459650	None:intergenic	40.0%
	GGGATATTCATTAATGGTGG+TGG	+	chr7.2:26459625-26459644	None:intergenic	40.0%
	GGTTATCGTTGGAGGAAATA+TGG	-	chr7.2:26460021-26460040	MS.gene85467:CDS	40.0%
	TTAGAGGAGAAAAGGGTTAG+AGG	-	chr7.2:26459922-26459941	MS.gene85467:CDS	40.0%
!	AAGAAGGCGTAAAGAAGAAG+GGG	+	chr7.2:26459681-26459700	None:intergenic	40.0%
!	AAGGTCTTGGTGATATTGAG+TGG	-	chr7.2:26459731-26459750	MS.gene85467:CDS	40.0%
!	AGGTCTTGGTGATATTGAGT+GGG	-	chr7.2:26459732-26459751	MS.gene85467:CDS	40.0%
!	CTTGTCTGAAATGCAAACCT+TGG	+	chr7.2:26459976-26459995	None:intergenic	40.0%
!!	CCTTTGGAATCACAAGATCA+AGG	-	chr7.2:26459712-26459731	MS.gene85467:CDS	40.0%
!!	GAGTGGGGAAACTTTTTCTT+AGG	-	chr7.2:26459748-26459767	MS.gene85467:CDS	40.0%
!!!	TGTTTTGAGTTTGTGGCGAT+GGG	+	chr7.2:26459645-26459664	None:intergenic	40.0%
!!!	TTGTTTTGAGTTTGTGGCGA+TGG	+	chr7.2:26459646-26459665	None:intergenic	40.0%
	AGGAGAAAAGGGTTAGAGGT+GGG	-	chr7.2:26459926-26459945	MS.gene85467:CDS	45.0%
	TCAAGAACAGCAAGTATCCC+AGG	-	chr7.2:26460055-26460074	MS.gene85467:CDS	45.0%
!	GGTCTTGGTGATATTGAGTG+GGG	-	chr7.2:26459733-26459752	MS.gene85467:CDS	45.0%
!	TGGATGATGGTTATCGTTGG+AGG	-	chr7.2:26460013-26460032	MS.gene85467:CDS	45.0%
!!	ATTAATGGTGGTGGTGGGTT+TGG	+	chr7.2:26459616-26459635	None:intergenic	45.0%
	GAGGAGAAAAGGGTTAGAGG+TGG	-	chr7.2:26459925-26459944	MS.gene85467:CDS	50.0%
!	CTTGCTTGTTGGGGATGCTA+AGG	-	chr7.2:26459786-26459805	MS.gene85467:CDS	50.0%
!!	AATGGTGGTGGTGGGTTTGG+TGG	+	chr7.2:26459613-26459632	None:intergenic	55.0%
!!	ATGGTGGTGGTGGGTTTGGT+GGG	+	chr7.2:26459612-26459631	None:intergenic	55.0%
!!	GTGGTGGGTTTGGTGGGTCT+TGG	+	chr7.2:26459606-26459625	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	26459595	26460101	26459595	ID=MS.gene85467
chr7.2	mRNA	26459595	26460101	26459595	ID=MS.gene85467.t1;Parent=MS.gene85467
chr7.2	exon	26459595	26460101	26459595	ID=MS.gene85467.t1.exon1;Parent=MS.gene85467.t1
chr7.2	CDS	26459595	26460101	26459595	ID=cds.MS.gene85467.t1;Parent=MS.gene85467.t1

Gene Sequence

>MS.gene85467

ATGGATAGCCAAGACCCACCAAACCCACCACCACCATTAATGAATATCCCATCGCCACAAACTCAAAACAACAACACTTCTCTCCCCTTCTTCTTTACGCCTTCTTTACAAAATTACCCTTTGGAATCACAAGATCAAGGTCTTGGTGATATTGAGTGGGGAAACTTTTTCTTAGGTCAAAACAACAATAACTTGCTTGTTGGGGATGCTAAGGAGACATTAAAGATTGACAATATTCAACAATGCACTTCATCTTCTTCTAATTTGGTAATTCAAAATAATGAAAGTGGGAGTTATCAACTACATGAAGAAGAAAAGGGAAACAAGTTAGAGGAGAAAAGGGTTAGAGGTGGGAGAGTGAAAAAGACAACAAAAGTGCCAAGGTTTGCATTTCAGACAAGAAGTGTTGATGATATTTTGGATGATGGTTATCGTTGGAGGAAATATGGACAAAAAGCTGTCAAGAACAGCAAGTATCCCAGGTATATACTACATGTATGTTTGTGA

Protein sequence

>MS.gene85467.t1

MDSQDPPNPPPPLMNIPSPQTQNNNTSLPFFFTPSLQNYPLESQDQGLGDIEWGNFFLGQNNNNLLVGDAKETLKIDNIQQCTSSSSNLVIQNNESGSYQLHEEEKGNKLEEKRVRGGRVKKTTKVPRFAFQTRSVDDILDDGYRWRKYGQKAVKNSKYPRYILHVCL