Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85544.t1 | KEH41338.1 | 82.4 | 153 | 12 | 1 | 1 | 138 | 1 | 153 | 4.50E-64 | 253.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85544.t1 | A0A072VIT9 | 82.4 | 153 | 12 | 1 | 1 | 138 | 1 | 153 | 3.2e-64 | 253.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene85544.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85544.t1 | MTR_1g048880 | 82.353 | 153 | 12 | 1 | 1 | 138 | 1 | 153 | 2.35e-88 | 254 |
| MS.gene85544.t1 | MTR_1g041455 | 54.491 | 167 | 47 | 4 | 1 | 138 | 1 | 167 | 2.40e-48 | 153 |
| MS.gene85544.t1 | MTR_5g056450 | 44.118 | 170 | 51 | 7 | 1 | 138 | 1 | 158 | 6.72e-28 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGAATAGAATCTTCTAATTG+AGG | - | 8647:3115-3134 | MS.gene85544:intergenic | 25.0% |
| !!! | TTCATGTTTTGAGTATCATT+TGG | - | 8647:3198-3217 | MS.gene85544:intergenic | 25.0% |
| GATACTCAAAACATGAATGA+TGG | + | 8647:3201-3220 | MS.gene85544:CDS | 30.0% | |
| TTGAGTGTTAAATCCAATGT+AGG | - | 8647:3381-3400 | MS.gene85544:intergenic | 30.0% | |
| ! | CATGATTACTTCTGAAAACT+TGG | - | 8647:3059-3078 | MS.gene85544:intergenic | 30.0% |
| ! | TCATCATCCTTTTTCTTGAT+AGG | - | 8647:3345-3364 | MS.gene85544:intergenic | 30.0% |
| ACCAAAGAAATTGTCCTTGA+GGG | - | 8647:3149-3168 | MS.gene85543:intergenic | 35.0% | |
| CGAGAAACCTATCAAGAAAA+AGG | + | 8647:3335-3354 | MS.gene85544:CDS | 35.0% | |
| CTCAAAACATGAATGATGGT+AGG | + | 8647:3205-3224 | MS.gene85544:CDS | 35.0% | |
| TACCAAAGAAATTGTCCTTG+AGG | - | 8647:3150-3169 | MS.gene85544:intergenic | 35.0% | |
| TCATGCATTAGTAACTCACA+TGG | + | 8647:3074-3093 | MS.gene85544:CDS | 35.0% | |
| TCATTTGGCAAAACATGTGA+CGG | - | 8647:3288-3307 | MS.gene85544:intergenic | 35.0% | |
| TGAAACATCATCATAGGATG+TGG | - | 8647:3246-3265 | MS.gene85544:intergenic | 35.0% | |
| ! | TCACATGTTTTGCCAAATGA+TGG | + | 8647:3288-3307 | MS.gene85544:CDS | 35.0% |
| ! | TCATTTGGAACATGAAAACC+TGG | - | 8647:3183-3202 | MS.gene85544:intergenic | 35.0% |
| ! | TTCTCGAGTTGCATGATATA+TGG | - | 8647:3321-3340 | MS.gene85544:intergenic | 35.0% |
| ACCCTCAAGGACAATTTCTT+TGG | + | 8647:3145-3164 | MS.gene85544:CDS | 40.0% | |
| ATCATCATAGGATGTGGTTG+AGG | - | 8647:3240-3259 | MS.gene85544:intergenic | 40.0% | |
| CCAACGACTTTAGTAACAAG+AGG | + | 8647:2995-3014 | MS.gene85544:CDS | 40.0% | |
| CCTCTTGTTACTAAAGTCGT+TGG | - | 8647:2998-3017 | MS.gene85544:intergenic | 40.0% | |
| GAGTTGCATGATATATGGCT+TGG | - | 8647:3316-3335 | MS.gene85544:intergenic | 40.0% | |
| GTTGGCTGAAACATCATCAT+AGG | - | 8647:3252-3271 | MS.gene85544:intergenic | 40.0% | |
| TTGGAACATGAAAACCTGGT+GGG | - | 8647:3179-3198 | MS.gene85544:intergenic | 40.0% | |
| ! | GACGGAATTTGGTTTCTTGT+TGG | - | 8647:3270-3289 | MS.gene85544:intergenic | 40.0% |
| ! | GCAAAACATGTGACGGAATT+TGG | - | 8647:3281-3300 | MS.gene85544:intergenic | 40.0% |
| ! | TTTGGAACATGAAAACCTGG+TGG | - | 8647:3180-3199 | MS.gene85544:intergenic | 40.0% |
| AATTGTCCTTGAGGGTTGAC+AGG | - | 8647:3141-3160 | MS.gene85543:intergenic | 45.0% | |
| CAACTCAGCTGTTCCTACAT+TGG | + | 8647:3365-3384 | MS.gene85544:CDS | 45.0% | |
| GAGGCATGATGTGCAATACT+CGG | + | 8647:3014-3033 | MS.gene85544:CDS | 45.0% | |
| TGGAACATGAAAACCTGGTG+GGG | - | 8647:3178-3197 | MS.gene85544:intergenic | 45.0% | |
| !! | TATGGCTTGGTGCCATCATT+TGG | - | 8647:3303-3322 | MS.gene85544:intergenic | 45.0% |
| ACTCACATGGAGTCTCACGA+AGG | + | 8647:3087-3106 | MS.gene85544:CDS | 50.0% | |
| CTCACATGGAGTCTCACGAA+GGG | + | 8647:3088-3107 | MS.gene85544:CDS | 50.0% | |
| CTTTGGTATAGTTCCCCACC+AGG | + | 8647:3162-3181 | MS.gene85544:CDS | 50.0% | |
| TCACATGGAGTCTCACGAAG+GGG | + | 8647:3089-3108 | MS.gene85544:CDS | 50.0% | |
| TCAGAACCTGTCAACCCTCA+AGG | + | 8647:3132-3151 | MS.gene85544:CDS | 50.0% | |
| GCAATACTCGGTCGCATGCA+GGG | + | 8647:3026-3045 | MS.gene85544:CDS | 55.0% | |
| TGCAATACTCGGTCGCATGC+AGG | + | 8647:3025-3044 | MS.gene85544:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 8647 | gene | 2991 | 3407 | 2991 | ID=MS.gene85544 |
| 8647 | mRNA | 2991 | 3407 | 2991 | ID=MS.gene85544.t1;Parent=MS.gene85544 |
| 8647 | exon | 2991 | 3407 | 2991 | ID=MS.gene85544.t1.exon1;Parent=MS.gene85544.t1 |
| 8647 | CDS | 2991 | 3407 | 2991 | ID=cds.MS.gene85544.t1;Parent=MS.gene85544.t1 |
| Gene Sequence |
| Protein sequence |
