Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85546.t1 | KEH41338.1 | 82.4 | 153 | 12 | 1 | 1 | 138 | 1 | 153 | 4.50E-64 | 253.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85546.t1 | A0A072VIT9 | 82.4 | 153 | 12 | 1 | 1 | 138 | 1 | 153 | 3.2e-64 | 253.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene85546.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85546.t1 | MTR_1g048880 | 82.353 | 153 | 12 | 1 | 1 | 138 | 1 | 153 | 2.35e-88 | 254 |
| MS.gene85546.t1 | MTR_1g041455 | 54.491 | 167 | 47 | 4 | 1 | 138 | 1 | 167 | 2.40e-48 | 153 |
| MS.gene85546.t1 | MTR_5g056450 | 44.118 | 170 | 51 | 7 | 1 | 138 | 1 | 158 | 6.72e-28 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGAATAGAATCTTCTAATTG+AGG | - | 8646:2872-2891 | MS.gene85546:intergenic | 25.0% |
| !!! | TTCATGTTTTGAGTATCATT+TGG | - | 8646:2955-2974 | MS.gene85546:intergenic | 25.0% |
| GATACTCAAAACATGAATGA+TGG | + | 8646:2958-2977 | MS.gene85546:CDS | 30.0% | |
| TTGAGTGTTAAATCCAATGT+AGG | - | 8646:3138-3157 | MS.gene85546:intergenic | 30.0% | |
| ! | CATGATTACTTCTGAAAACT+TGG | - | 8646:2816-2835 | MS.gene85546:intergenic | 30.0% |
| ! | TCATCATCCTTTTTCTTGAT+AGG | - | 8646:3102-3121 | MS.gene85546:intergenic | 30.0% |
| ACCAAAGAAATTGTCCTTGA+GGG | - | 8646:2906-2925 | MS.gene85545:intergenic | 35.0% | |
| CGAGAAACCTATCAAGAAAA+AGG | + | 8646:3092-3111 | MS.gene85546:CDS | 35.0% | |
| CTCAAAACATGAATGATGGT+AGG | + | 8646:2962-2981 | MS.gene85546:CDS | 35.0% | |
| TACCAAAGAAATTGTCCTTG+AGG | - | 8646:2907-2926 | MS.gene85546:intergenic | 35.0% | |
| TCATGCATTAGTAACTCACA+TGG | + | 8646:2831-2850 | MS.gene85546:CDS | 35.0% | |
| TCATTTGGCAAAACATGTGA+CGG | - | 8646:3045-3064 | MS.gene85546:intergenic | 35.0% | |
| TGAAACATCATCATAGGATG+TGG | - | 8646:3003-3022 | MS.gene85546:intergenic | 35.0% | |
| ! | TCACATGTTTTGCCAAATGA+TGG | + | 8646:3045-3064 | MS.gene85546:CDS | 35.0% |
| ! | TCATTTGGAACATGAAAACC+TGG | - | 8646:2940-2959 | MS.gene85546:intergenic | 35.0% |
| ! | TTCTCGAGTTGCATGATATA+TGG | - | 8646:3078-3097 | MS.gene85546:intergenic | 35.0% |
| ACCCTCAAGGACAATTTCTT+TGG | + | 8646:2902-2921 | MS.gene85546:CDS | 40.0% | |
| ATCATCATAGGATGTGGTTG+AGG | - | 8646:2997-3016 | MS.gene85546:intergenic | 40.0% | |
| CCAACGACTTTAGTAACAAG+AGG | + | 8646:2752-2771 | MS.gene85546:CDS | 40.0% | |
| CCTCTTGTTACTAAAGTCGT+TGG | - | 8646:2755-2774 | MS.gene85546:intergenic | 40.0% | |
| GAGTTGCATGATATATGGCT+TGG | - | 8646:3073-3092 | MS.gene85546:intergenic | 40.0% | |
| GTTGGCTGAAACATCATCAT+AGG | - | 8646:3009-3028 | MS.gene85546:intergenic | 40.0% | |
| TTGGAACATGAAAACCTGGT+GGG | - | 8646:2936-2955 | MS.gene85546:intergenic | 40.0% | |
| ! | GACGGAATTTGGTTTCTTGT+TGG | - | 8646:3027-3046 | MS.gene85546:intergenic | 40.0% |
| ! | GCAAAACATGTGACGGAATT+TGG | - | 8646:3038-3057 | MS.gene85546:intergenic | 40.0% |
| ! | TTTGGAACATGAAAACCTGG+TGG | - | 8646:2937-2956 | MS.gene85546:intergenic | 40.0% |
| AATTGTCCTTGAGGGTTGAC+AGG | - | 8646:2898-2917 | MS.gene85545:intergenic | 45.0% | |
| CAACTCAGCTGTTCCTACAT+TGG | + | 8646:3122-3141 | MS.gene85546:CDS | 45.0% | |
| GAGGCATGATGTGCAATACT+CGG | + | 8646:2771-2790 | MS.gene85546:CDS | 45.0% | |
| TGGAACATGAAAACCTGGTG+GGG | - | 8646:2935-2954 | MS.gene85546:intergenic | 45.0% | |
| !! | TATGGCTTGGTGCCATCATT+TGG | - | 8646:3060-3079 | MS.gene85546:intergenic | 45.0% |
| ACTCACATGGAGTCTCACGA+AGG | + | 8646:2844-2863 | MS.gene85546:CDS | 50.0% | |
| CTCACATGGAGTCTCACGAA+GGG | + | 8646:2845-2864 | MS.gene85546:CDS | 50.0% | |
| CTTTGGTATAGTTCCCCACC+AGG | + | 8646:2919-2938 | MS.gene85546:CDS | 50.0% | |
| TCACATGGAGTCTCACGAAG+GGG | + | 8646:2846-2865 | MS.gene85546:CDS | 50.0% | |
| TCAGAACCTGTCAACCCTCA+AGG | + | 8646:2889-2908 | MS.gene85546:CDS | 50.0% | |
| GCAATACTCGGTCGCATGCA+GGG | + | 8646:2783-2802 | MS.gene85546:CDS | 55.0% | |
| TGCAATACTCGGTCGCATGC+AGG | + | 8646:2782-2801 | MS.gene85546:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 8646 | gene | 2748 | 3164 | 2748 | ID=MS.gene85546 |
| 8646 | mRNA | 2748 | 3164 | 2748 | ID=MS.gene85546.t1;Parent=MS.gene85546 |
| 8646 | exon | 2748 | 3164 | 2748 | ID=MS.gene85546.t1.exon1;Parent=MS.gene85546.t1 |
| 8646 | CDS | 2748 | 3164 | 2748 | ID=cds.MS.gene85546.t1;Parent=MS.gene85546.t1 |
| Gene Sequence |
| Protein sequence |
