Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85548.t1 | KEH41338.1 | 82.4 | 153 | 12 | 1 | 1 | 138 | 1 | 153 | 4.50E-64 | 253.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85548.t1 | A0A072VIT9 | 82.4 | 153 | 12 | 1 | 1 | 138 | 1 | 153 | 3.2e-64 | 253.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene85548.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85548.t1 | MTR_1g048880 | 82.353 | 153 | 12 | 1 | 1 | 138 | 1 | 153 | 2.35e-88 | 254 |
| MS.gene85548.t1 | MTR_1g041455 | 54.491 | 167 | 47 | 4 | 1 | 138 | 1 | 167 | 2.40e-48 | 153 |
| MS.gene85548.t1 | MTR_5g056450 | 44.118 | 170 | 51 | 7 | 1 | 138 | 1 | 158 | 6.72e-28 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGAATAGAATCTTCTAATTG+AGG | - | 8645:2210-2229 | MS.gene85548:intergenic | 25.0% |
| !!! | TTCATGTTTTGAGTATCATT+TGG | - | 8645:2293-2312 | MS.gene85548:intergenic | 25.0% |
| GATACTCAAAACATGAATGA+TGG | + | 8645:2296-2315 | MS.gene85548:CDS | 30.0% | |
| TTGAGTGTTAAATCCAATGT+AGG | - | 8645:2476-2495 | MS.gene85548:intergenic | 30.0% | |
| ! | CATGATTACTTCTGAAAACT+TGG | - | 8645:2154-2173 | MS.gene85548:intergenic | 30.0% |
| ! | TCATCATCCTTTTTCTTGAT+AGG | - | 8645:2440-2459 | MS.gene85548:intergenic | 30.0% |
| ACCAAAGAAATTGTCCTTGA+GGG | - | 8645:2244-2263 | MS.gene85547:intergenic | 35.0% | |
| CGAGAAACCTATCAAGAAAA+AGG | + | 8645:2430-2449 | MS.gene85548:CDS | 35.0% | |
| CTCAAAACATGAATGATGGT+AGG | + | 8645:2300-2319 | MS.gene85548:CDS | 35.0% | |
| TACCAAAGAAATTGTCCTTG+AGG | - | 8645:2245-2264 | MS.gene85548:intergenic | 35.0% | |
| TCATGCATTAGTAACTCACA+TGG | + | 8645:2169-2188 | MS.gene85548:CDS | 35.0% | |
| TCATTTGGCAAAACATGTGA+CGG | - | 8645:2383-2402 | MS.gene85548:intergenic | 35.0% | |
| TGAAACATCATCATAGGATG+TGG | - | 8645:2341-2360 | MS.gene85548:intergenic | 35.0% | |
| ! | TCACATGTTTTGCCAAATGA+TGG | + | 8645:2383-2402 | MS.gene85548:CDS | 35.0% |
| ! | TCATTTGGAACATGAAAACC+TGG | - | 8645:2278-2297 | MS.gene85548:intergenic | 35.0% |
| ! | TTCTCGAGTTGCATGATATA+TGG | - | 8645:2416-2435 | MS.gene85548:intergenic | 35.0% |
| ACCCTCAAGGACAATTTCTT+TGG | + | 8645:2240-2259 | MS.gene85548:CDS | 40.0% | |
| ATCATCATAGGATGTGGTTG+AGG | - | 8645:2335-2354 | MS.gene85548:intergenic | 40.0% | |
| CCAACGACTTTAGTAACAAG+AGG | + | 8645:2090-2109 | MS.gene85548:CDS | 40.0% | |
| CCTCTTGTTACTAAAGTCGT+TGG | - | 8645:2093-2112 | MS.gene85548:intergenic | 40.0% | |
| GAGTTGCATGATATATGGCT+TGG | - | 8645:2411-2430 | MS.gene85548:intergenic | 40.0% | |
| GTTGGCTGAAACATCATCAT+AGG | - | 8645:2347-2366 | MS.gene85548:intergenic | 40.0% | |
| TTGGAACATGAAAACCTGGT+GGG | - | 8645:2274-2293 | MS.gene85548:intergenic | 40.0% | |
| ! | GACGGAATTTGGTTTCTTGT+TGG | - | 8645:2365-2384 | MS.gene85548:intergenic | 40.0% |
| ! | GCAAAACATGTGACGGAATT+TGG | - | 8645:2376-2395 | MS.gene85548:intergenic | 40.0% |
| ! | TTTGGAACATGAAAACCTGG+TGG | - | 8645:2275-2294 | MS.gene85548:intergenic | 40.0% |
| AATTGTCCTTGAGGGTTGAC+AGG | - | 8645:2236-2255 | MS.gene85547:intergenic | 45.0% | |
| CAACTCAGCTGTTCCTACAT+TGG | + | 8645:2460-2479 | MS.gene85548:CDS | 45.0% | |
| GAGGCATGATGTGCAATACT+CGG | + | 8645:2109-2128 | MS.gene85548:CDS | 45.0% | |
| TGGAACATGAAAACCTGGTG+GGG | - | 8645:2273-2292 | MS.gene85548:intergenic | 45.0% | |
| !! | TATGGCTTGGTGCCATCATT+TGG | - | 8645:2398-2417 | MS.gene85548:intergenic | 45.0% |
| ACTCACATGGAGTCTCACGA+AGG | + | 8645:2182-2201 | MS.gene85548:CDS | 50.0% | |
| CTCACATGGAGTCTCACGAA+GGG | + | 8645:2183-2202 | MS.gene85548:CDS | 50.0% | |
| CTTTGGTATAGTTCCCCACC+AGG | + | 8645:2257-2276 | MS.gene85548:CDS | 50.0% | |
| TCACATGGAGTCTCACGAAG+GGG | + | 8645:2184-2203 | MS.gene85548:CDS | 50.0% | |
| TCAGAACCTGTCAACCCTCA+AGG | + | 8645:2227-2246 | MS.gene85548:CDS | 50.0% | |
| GCAATACTCGGTCGCATGCA+GGG | + | 8645:2121-2140 | MS.gene85548:CDS | 55.0% | |
| TGCAATACTCGGTCGCATGC+AGG | + | 8645:2120-2139 | MS.gene85548:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 8645 | gene | 2086 | 2502 | 2086 | ID=MS.gene85548 |
| 8645 | mRNA | 2086 | 2502 | 2086 | ID=MS.gene85548.t1;Parent=MS.gene85548 |
| 8645 | exon | 2086 | 2502 | 2086 | ID=MS.gene85548.t1.exon1;Parent=MS.gene85548.t1 |
| 8645 | CDS | 2086 | 2502 | 2086 | ID=cds.MS.gene85548.t1;Parent=MS.gene85548.t1 |
| Gene Sequence |
| Protein sequence |
