AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85551

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85551.t1 MTR_1g075890 81.776 653 58 3 1 592 1 653 0.0 1016
MS.gene85551.t1 MTR_3g464260 70.072 558 78 8 37 592 29 499 0.0 737
MS.gene85551.t1 MTR_3g464280 64.561 570 120 6 1 564 1 494 0.0 662
MS.gene85551.t1 MTR_5g071070 52.000 675 232 14 1 591 1 667 0.0 617
MS.gene85551.t1 MTR_5g071070 51.607 591 200 12 81 591 1 585 0.0 531
MS.gene85551.t1 MTR_5g082830 45.152 660 241 17 19 586 13 643 4.26e-176 515
MS.gene85551.t1 MTR_7g092070 40.875 663 260 19 30 586 44 680 1.30e-144 436
MS.gene85551.t1 MTR_3g070880 43.448 580 199 17 31 583 8 485 4.15e-136 407
MS.gene85551.t1 MTR_3g464290 55.134 448 151 10 168 591 39 460 1.04e-131 394
MS.gene85551.t1 MTR_3g029590 39.430 667 275 24 30 588 42 687 2.46e-127 391
MS.gene85551.t1 MTR_3g464270 53.406 367 142 6 228 582 8 357 1.36e-114 345
MS.gene85551.t1 MTR_3g435590 49.030 361 173 5 30 380 29 388 3.27e-106 328
MS.gene85551.t1 MTR_1g050438 42.117 463 228 11 32 458 14 472 7.14e-106 331
MS.gene85551.t1 MTR_0728s0020 42.117 463 228 11 32 458 30 488 2.81e-105 330
MS.gene85551.t1 MTR_3g435680 43.223 273 127 6 136 384 44 312 2.27e-65 219
MS.gene85551.t1 MTR_8g102100 54.268 164 69 1 225 382 77 240 2.22e-55 192
MS.gene85551.t1 MTR_6g090380 59.848 132 44 1 200 331 83 205 7.14e-53 185
MS.gene85551.t1 MTR_3g103960 59.701 134 52 2 219 350 43 176 1.81e-50 176
MS.gene85551.t1 MTR_2g090605 62.162 111 42 0 229 339 77 187 4.54e-48 173
MS.gene85551.t1 MTR_4g078660 57.944 107 45 0 225 331 56 162 2.57e-45 163
MS.gene85551.t1 MTR_1g081420 70.968 62 9 2 484 536 167 228 1.39e-15 76.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85551.t1 AT5G58620 48.328 598 247 19 27 583 8 584 2.37e-178 518
MS.gene85551.t1 AT3G55980 45.757 601 254 14 1 583 1 547 1.81e-163 479
MS.gene85551.t1 AT3G55980 45.757 601 254 14 1 583 68 614 1.30e-162 479
MS.gene85551.t1 AT2G40140 45.289 605 259 16 1 583 1 555 6.61e-161 473
MS.gene85551.t1 AT2G40140 45.289 605 259 16 1 583 1 555 6.61e-161 473
MS.gene85551.t1 AT5G12850 41.789 615 257 22 25 551 34 635 8.50e-136 412
MS.gene85551.t1 AT2G41900 46.383 470 193 7 25 453 40 491 9.28e-136 412
MS.gene85551.t1 AT5G07500 61.157 121 47 0 211 331 34 154 3.12e-53 182
MS.gene85551.t1 AT4G29190 51.744 172 61 3 218 382 68 224 4.87e-53 185
MS.gene85551.t1 AT2G19810 62.295 122 44 1 214 333 61 182 1.94e-51 181
MS.gene85551.t1 AT2G25900 58.140 129 54 0 225 353 86 214 3.62e-51 179
MS.gene85551.t1 AT2G25900 58.140 129 54 0 225 353 86 214 3.62e-51 179
MS.gene85551.t1 AT1G03790 52.258 155 66 3 196 346 53 203 4.23e-48 173
MS.gene85551.t1 AT5G44260 57.812 128 49 2 207 333 42 165 2.42e-46 167

Find 139 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACTTCATTGTGCTGTTTC+TGG 0.111884 1.3:+54424208 MS.gene85551:CDS
TGCTGCTTTGTTTGGAAGTA+TGG 0.164133 1.3:+54424114 MS.gene85551:CDS
ACCTTCTGCCTTCTCTGGTT+GGG 0.217111 1.3:+54425431 MS.gene85551:CDS
CTTGTCAAACTGGTTTGATT+AGG 0.230886 1.3:+54423894 MS.gene85551:CDS
GGTGTGTTGTCTTATATTCT+TGG 0.293124 1.3:+54424136 MS.gene85551:CDS
TTGTCAAACTGGTTTGATTA+GGG 0.297404 1.3:+54423895 MS.gene85551:CDS
GAACTTGAAAATCGCCTTAC+AGG 0.298409 1.3:+54425138 MS.gene85551:CDS
GTCTCCCTTGCCCTGAGTTT+CGG 0.301992 1.3:+54424683 MS.gene85551:CDS
GGCGATTTCCTTAAGGAAAT+AGG 0.305515 1.3:+54424418 MS.gene85551:CDS
TATATAGTACAGATGAATTT+AGG 0.309340 1.3:+54424539 MS.gene85551:CDS
CTTATGGTTGCTGCTTTGTT+TGG 0.322501 1.3:+54424106 MS.gene85551:CDS
AATAGAAAACCTTGTCAAAC+TGG 0.336343 1.3:+54423884 MS.gene85551:CDS
GAACACAAACCAGCAACTTT+GGG 0.341369 1.3:+54425263 MS.gene85551:CDS
GAGTGCCCCTTTGTTCATCC+TGG 0.343455 1.3:+54424616 MS.gene85551:CDS
ATGGAACCTTCTGCCTTCTC+TGG 0.354986 1.3:+54425426 MS.gene85551:CDS
TGTTGATCAAATTCCATTGT+TGG 0.356576 1.3:-54425621 None:intergenic
GAACGCCATTACATCATCTT+TGG 0.357587 1.3:-54423981 None:intergenic
AAGGATTGCCCCAAAGTTGC+TGG 0.360358 1.3:-54425272 None:intergenic
GCAAACCCTTCTCGTGCATC+TGG 0.369951 1.3:-54424809 None:intergenic
CTTACCAATTCAAATGTCAT+TGG 0.371079 1.3:+54425300 MS.gene85551:CDS
GTCTTGCATCTCCTTCAAAT+AGG 0.375795 1.3:+54425160 MS.gene85551:CDS
AGAACACAAACCAGCAACTT+TGG 0.377562 1.3:+54425262 MS.gene85551:CDS
TAAGGAAATAGGTTTCAAAA+TGG 0.383529 1.3:+54424429 MS.gene85551:CDS
AACCTTCTGCCTTCTCTGGT+TGG 0.384069 1.3:+54425430 MS.gene85551:CDS
GAAGGTTGGATCACATGAAA+TGG 0.385080 1.3:+54424063 MS.gene85551:CDS
TTCACCAAGTGCTTCTTCAA+TGG 0.396899 1.3:+54424915 MS.gene85551:CDS
TGAAGAGAGGACACCTCTTA+TGG 0.399251 1.3:+54424090 MS.gene85551:CDS
GTGAATCAACCCAAGATTCA+TGG 0.407246 1.3:-54425584 None:intergenic
TGCTGCGCTCAATAAAGCTT+TGG 0.407302 1.3:-54425362 None:intergenic
TCGGTTTCTAAGTGTATCTT+CGG 0.409318 1.3:+54424334 MS.gene85551:CDS
TTTGAATTGGTAAGATCAAA+AGG 0.409476 1.3:-54425291 None:intergenic
TTGTCTTATATTCTTGGAAC+CGG 0.410886 1.3:+54424142 MS.gene85551:CDS
ACTTCTGCAAACCTATTTGA+AGG 0.410959 1.3:-54425171 None:intergenic
CTAAGTGTATCTTCGGTTCA+AGG 0.415813 1.3:+54424341 MS.gene85551:CDS
CCTTCTGCCTTCTCTGGTTG+GGG 0.417819 1.3:+54425432 MS.gene85551:CDS
TCAAAGTGAAGCCTTGTTCA+AGG 0.431149 1.3:+54424572 MS.gene85551:CDS
TCTGGTTGGGGCTCTCCTGA+TGG 0.432980 1.3:+54425444 MS.gene85551:CDS
AGTGCCCCTTTGTTCATCCT+GGG 0.433195 1.3:+54424617 MS.gene85551:CDS
TGATCCAATGACATTTGAAT+TGG 0.433935 1.3:-54425304 None:intergenic
TGACGATGGCGATTTCCTTA+AGG 0.439025 1.3:+54424411 MS.gene85551:CDS
GTCAGGTAGGGATGGATCAA+TGG 0.440308 1.3:-54424503 None:intergenic
AACAAAGCAGCAACCATAAG+AGG 0.442942 1.3:-54424103 None:intergenic
CAAGGGCTTACTCTCATGAT+TGG 0.443825 1.3:+54424590 MS.gene85551:CDS
CCCTTGCCCTGAGTTTCGGA+CGG 0.446696 1.3:+54424687 MS.gene85551:CDS
ACCGAGCGTGCGGCTCAGAT+AGG 0.449079 1.3:+54424179 MS.gene85551:CDS
GCTGCTTTGTTTGGAAGTAT+GGG 0.450481 1.3:+54424115 MS.gene85551:CDS
TTGTGGTATGGAAGGAAGGT+TGG 0.451300 1.3:+54424049 MS.gene85551:CDS
CATGATCCCGTCCGAAACTC+AGG 0.451924 1.3:-54424694 None:intergenic
TAATCTTTCTTCTCAGTTCG+AGG 0.455693 1.3:-54424478 None:intergenic
TATATTCCATTCTTAATGTC+AGG 0.462194 1.3:-54424520 None:intergenic
TTCTCCCCAGGATGAACAAA+GGG 0.463697 1.3:-54424622 None:intergenic
CTTCTTGGCATCTGAAGGGT+AGG 0.464649 1.3:-54425069 None:intergenic
ATCTTCTTCTTGGCATCTGA+AGG 0.468218 1.3:-54425074 None:intergenic
TACAACGGTCGAAGCTCCTC+TGG 0.468452 1.3:-54424847 None:intergenic
TTCTGCTAGCTCAGTTGCCA+TGG 0.471344 1.3:+54425407 MS.gene85551:CDS
AGGTGAAGGTAGAGCAGAAC+CGG 0.476212 1.3:-54424878 None:intergenic
CCCTATCTGAGCCGCACGCT+CGG 0.476697 1.3:-54424180 None:intergenic
ATTGAGTTCATTGCCGCGGA+TGG 0.478340 1.3:-54425481 None:intergenic
GTTGATTCACTTGTGAATGA+TGG 0.479955 1.3:+54425597 MS.gene85551:CDS
GCAGCATCATCGCCTACGGC+TGG 0.487982 1.3:+54425006 MS.gene85551:CDS
CGACCGTTGTATGCTTCTAC+CGG 0.490079 1.3:+54424859 MS.gene85551:CDS
AAGGTTGGATCACATGAAAT+GGG 0.495696 1.3:+54424064 MS.gene85551:CDS
AAGTAGGGTTGTGGTATGGA+AGG 0.496075 1.3:+54424041 MS.gene85551:CDS
AGCCTTGTTCTGTATTGAGC+AGG 0.498591 1.3:-54424772 None:intergenic
TCCCTACCTGACATTAAGAA+TGG 0.501206 1.3:+54424514 MS.gene85551:CDS
CCTTGCCCTGAGTTTCGGAC+GGG 0.502111 1.3:+54424688 MS.gene85551:CDS
ATACTGAAAGGCTTACTAGA+AGG 0.507130 1.3:+54424370 MS.gene85551:CDS
TTCAGCCAAAGATGATGTAA+TGG 0.508817 1.3:+54423976 MS.gene85551:CDS
TCCATTCTTAATGTCAGGTA+GGG 0.510904 1.3:-54424515 None:intergenic
TCAGGTAGGGATGGATCAAT+GGG 0.513171 1.3:-54424502 None:intergenic
AGGGTTGTGGTATGGAAGGA+AGG 0.514853 1.3:+54424045 MS.gene85551:CDS
CAAAGTGAAGCCTTGTTCAA+GGG 0.517860 1.3:+54424573 MS.gene85551:CDS
CGGCGCCTTGCATTCTCCCC+AGG 0.518859 1.3:-54424634 None:intergenic
TTCTTAATGTCAGGTAGGGA+TGG 0.519031 1.3:-54424511 None:intergenic
GGAAATAGGTTTCAAAATGG+TGG 0.519603 1.3:+54424432 MS.gene85551:CDS
GATCATGATGTTGATGAAGT+AGG 0.526063 1.3:+54424025 MS.gene85551:CDS
GTATCTTCGGTTCAAGGAAG+AGG 0.528667 1.3:+54424347 MS.gene85551:CDS
TTCATCCTGGGGAGAATGCA+AGG 0.528936 1.3:+54424629 MS.gene85551:CDS
TTCTTGGCATCTGAAGGGTA+GGG 0.529780 1.3:-54425068 None:intergenic
ATCATGATGTTGATGAAGTA+GGG 0.530587 1.3:+54424026 MS.gene85551:CDS
CCCGTCCGAAACTCAGGGCA+AGG 0.534270 1.3:-54424688 None:intergenic
AAGGAGATGCAAGACCTGTA+AGG 0.541933 1.3:-54425152 None:intergenic
CAGATTGTATCATAGAAGAC+GGG 0.542671 1.3:-54424957 None:intergenic
AGGAGAGCCCCAACCAGAGA+AGG 0.547335 1.3:-54425439 None:intergenic
CCGAGCGTGCGGCTCAGATA+GGG 0.547437 1.3:+54424180 MS.gene85551:CDS
CTCCTGATGGAAAATTAGAC+TGG 0.548612 1.3:+54425457 MS.gene85551:CDS
TCTGTTACCGTTTGCATCAG+CGG 0.549170 1.3:-54424293 None:intergenic
ATGCAGCGACTTGATTATCT+CGG 0.549828 1.3:+54424315 MS.gene85551:CDS
CAGAAGTAGCACTTCAACAA+TGG 0.550435 1.3:+54425521 MS.gene85551:CDS
AATGATATTTCCTCGGATCA+CGG 0.552863 1.3:-54424654 None:intergenic
GTTAGTTCCGCTGATGCAAA+CGG 0.558347 1.3:+54424286 MS.gene85551:CDS
ATCTGCAGCATCATCGCCTA+CGG 0.562339 1.3:+54425002 MS.gene85551:CDS
TCGGTTAACATCAACGCGAC+CGG 0.566776 1.3:-54424161 None:intergenic
AGAAACCAGATGCACGAGAA+GGG 0.568595 1.3:+54424804 MS.gene85551:CDS
CATGTGTAATGATATTTCCT+CGG 0.570791 1.3:-54424661 None:intergenic
GATGAAGTAGGGTTGTGGTA+TGG 0.576535 1.3:+54424037 MS.gene85551:CDS
TCTTCTTCTTGGCATCTGAA+GGG 0.578697 1.3:-54425073 None:intergenic
GTGCCCCTTTGTTCATCCTG+GGG 0.583668 1.3:+54424618 MS.gene85551:CDS
TTAGAATATGATGTAGGTGA+AGG 0.584198 1.3:-54424892 None:intergenic
CTAATCAAACCAGTTTGACA+AGG 0.588552 1.3:-54423893 None:intergenic
AGAGAAGGCAGAAGGTTCCA+TGG 0.589015 1.3:-54425424 None:intergenic
AAGAAACCAGATGCACGAGA+AGG 0.591165 1.3:+54424803 MS.gene85551:CDS
ATACAGAACAAGGCTTTGCA+AGG 0.591927 1.3:+54424780 MS.gene85551:CDS
GCAATGAACTCAATAAAATG+AGG 0.593274 1.3:+54425490 MS.gene85551:CDS
GTGGAGCAGCAACAAGATGT+AGG 0.593275 1.3:+54424451 MS.gene85551:CDS
CCCCAACCAGAGAAGGCAGA+AGG 0.594817 1.3:-54425432 None:intergenic
ATGCAAGGCGCCGTGATCCG+AGG 0.595327 1.3:+54424644 MS.gene85551:CDS
TTCCATTCTTAATGTCAGGT+AGG 0.597572 1.3:-54424516 None:intergenic
GTAGGCGATGATGCTGCAGA+TGG 0.600318 1.3:-54425000 None:intergenic
TGCCACATGGTTCCAGCCGT+AGG 0.601454 1.3:-54425018 None:intergenic
ACAGATTGTATCATAGAAGA+CGG 0.604472 1.3:-54424958 None:intergenic
GAATCCATTGAAGAAGCACT+TGG 0.606204 1.3:-54424919 None:intergenic
CCGTCCGAAACTCAGGGCAA+GGG 0.609374 1.3:-54424687 None:intergenic
CGGACGGGATCATGCAACAA+AGG 0.611380 1.3:+54424703 MS.gene85551:CDS
TTGTTCTATTGCTTCTGTCG+AGG 0.614035 1.3:+54424231 MS.gene85551:CDS
TGAGAGTAAGCCCTTGAACA+AGG 0.618008 1.3:-54424583 None:intergenic
GGTGAATTAGAATATGATGT+AGG 0.621500 1.3:-54424898 None:intergenic
ATGGCAACTGAGCTAGCAGA+AGG 0.621567 1.3:-54425405 None:intergenic
AACACAAACCAGCAACTTTG+GGG 0.626505 1.3:+54425264 MS.gene85551:CDS
AAAACAAAAGTCATGTGCAA+CGG 0.630659 1.3:+54423857 None:intergenic
ATTCTCCCCAGGATGAACAA+AGG 0.634474 1.3:-54424623 None:intergenic
ATGTTGATGAAGTAGGGTTG+TGG 0.634550 1.3:+54424032 MS.gene85551:CDS
ATGATCCCGTCCGAAACTCA+GGG 0.634829 1.3:-54424693 None:intergenic
ATCCTGCTCAATACAGAACA+AGG 0.635511 1.3:+54424770 MS.gene85551:CDS
CTGAATCTGAGTCTGCCACA+TGG 0.636502 1.3:-54425031 None:intergenic
TGTGAATGATGGTCCAACAA+TGG 0.642483 1.3:+54425608 MS.gene85551:CDS
ATGAAATGGGATATGAAGAG+AGG 0.647449 1.3:+54424077 MS.gene85551:CDS
GTTGATGTTAACCGAGCGTG+CGG 0.649710 1.3:+54424169 MS.gene85551:CDS
GATGCTGCAGATGGAGTCAA+AGG 0.650089 1.3:-54424991 None:intergenic
AAATTAGACTGGTCCATCCG+CGG 0.659387 1.3:+54425468 MS.gene85551:CDS
GCTTTGGCTCTGCTTCGAAG+AGG 0.660152 1.3:-54425346 None:intergenic
TCAAGGAAGAGGATACTGAA+AGG 0.669438 1.3:+54424358 MS.gene85551:CDS
CGCCTACGGCTGGAACCATG+TGG 0.694097 1.3:+54425016 MS.gene85551:CDS
GCTGCAGATGGAGTCAAAGG+TGG 0.696337 1.3:-54424988 None:intergenic
AGATTGTATCATAGAAGACG+GGG 0.721883 1.3:-54424956 None:intergenic
TCTCCCCAGGATGAACAAAG+GGG 0.731224 1.3:-54424621 None:intergenic
GATGCTTGTGAATATGCACA+TGG 0.757069 1.3:+54424727 MS.gene85551:CDS
GATGATGTTGATGATGACGA+TGG 0.765181 1.3:+54424397 MS.gene85551:CDS
GAACCGGTAGAAGCATACAA+CGG 0.780700 1.3:-54424862 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATAGTACAGATGAATTT+AGG + chr1.3:54424539-54424558 MS.gene85551:CDS 20.0%
! TAAGGAAATAGGTTTCAAAA+TGG + chr1.3:54424429-54424448 MS.gene85551:CDS 25.0%
! TATATTCCATTCTTAATGTC+AGG - chr1.3:54424523-54424542 None:intergenic 25.0%
! TCATGGCTTAAAAATATATC+TGG - chr1.3:54425570-54425589 None:intergenic 25.0%
! TTTGAATTGGTAAGATCAAA+AGG - chr1.3:54425294-54425313 None:intergenic 25.0%
!! AAAATGTTTTCATGGTTAGT+AGG - chr1.3:54425201-54425220 None:intergenic 25.0%
!! ACTAACCATGAAAACATTTT+CGG + chr1.3:54425201-54425220 MS.gene85551:CDS 25.0%
!!! ATTTTTAAGCCATGAATCTT+GGG + chr1.3:54425575-54425594 MS.gene85551:CDS 25.0%
!!! TATTTTTAAGCCATGAATCT+TGG + chr1.3:54425574-54425593 MS.gene85551:CDS 25.0%
!!! TTTTGAAACCTATTTCCTTA+AGG - chr1.3:54424429-54424448 None:intergenic 25.0%
AATAGAAAACCTTGTCAAAC+TGG + chr1.3:54423884-54423903 MS.gene85551:CDS 30.0%
ACAGATTGTATCATAGAAGA+CGG - chr1.3:54424961-54424980 None:intergenic 30.0%
CATGTGTAATGATATTTCCT+CGG - chr1.3:54424664-54424683 None:intergenic 30.0%
CTTACCAATTCAAATGTCAT+TGG + chr1.3:54425300-54425319 MS.gene85551:CDS 30.0%
GCAATGAACTCAATAAAATG+AGG + chr1.3:54425490-54425509 MS.gene85551:CDS 30.0%
GGTGAATTAGAATATGATGT+AGG - chr1.3:54424901-54424920 None:intergenic 30.0%
TGATCCAATGACATTTGAAT+TGG - chr1.3:54425307-54425326 None:intergenic 30.0%
TGTTGATCAAATTCCATTGT+TGG - chr1.3:54425624-54425643 None:intergenic 30.0%
TTAGAATATGATGTAGGTGA+AGG - chr1.3:54424895-54424914 None:intergenic 30.0%
TTGTCTTATATTCTTGGAAC+CGG + chr1.3:54424142-54424161 MS.gene85551:CDS 30.0%
! ATCATGATGTTGATGAAGTA+GGG + chr1.3:54424026-54424045 MS.gene85551:CDS 30.0%
! CTAACCATGAAAACATTTTC+GGG + chr1.3:54425202-54425221 MS.gene85551:CDS 30.0%
!! TTGTCAAACTGGTTTGATTA+GGG + chr1.3:54423895-54423914 MS.gene85551:CDS 30.0%
!!! GCAGTTTTAAATCTTCTTCT+TGG - chr1.3:54425087-54425106 None:intergenic 30.0%
AAGGTTGGATCACATGAAAT+GGG + chr1.3:54424064-54424083 MS.gene85551:CDS 35.0%
AATGATATTTCCTCGGATCA+CGG - chr1.3:54424657-54424676 None:intergenic 35.0%
ACTTCTGCAAACCTATTTGA+AGG - chr1.3:54425174-54425193 None:intergenic 35.0%
AGATTGTATCATAGAAGACG+GGG - chr1.3:54424959-54424978 None:intergenic 35.0%
ATACTGAAAGGCTTACTAGA+AGG + chr1.3:54424370-54424389 MS.gene85551:CDS 35.0%
ATGAAATGGGATATGAAGAG+AGG + chr1.3:54424077-54424096 MS.gene85551:CDS 35.0%
CAGATTGTATCATAGAAGAC+GGG - chr1.3:54424960-54424979 None:intergenic 35.0%
CTAATCAAACCAGTTTGACA+AGG - chr1.3:54423896-54423915 None:intergenic 35.0%
GGAAATAGGTTTCAAAATGG+TGG + chr1.3:54424432-54424451 MS.gene85551:CDS 35.0%
GGTGTGTTGTCTTATATTCT+TGG + chr1.3:54424136-54424155 MS.gene85551:CDS 35.0%
TAATCTTTCTTCTCAGTTCG+AGG - chr1.3:54424481-54424500 None:intergenic 35.0%
TCCATTCTTAATGTCAGGTA+GGG - chr1.3:54424518-54424537 None:intergenic 35.0%
TCGGTTTCTAAGTGTATCTT+CGG + chr1.3:54424334-54424353 MS.gene85551:CDS 35.0%
TTCAGCCAAAGATGATGTAA+TGG + chr1.3:54423976-54423995 MS.gene85551:CDS 35.0%
TTCCATTCTTAATGTCAGGT+AGG - chr1.3:54424519-54424538 None:intergenic 35.0%
! ATGACCCGAAAATGTTTTCA+TGG - chr1.3:54425209-54425228 None:intergenic 35.0%
! GATCATGATGTTGATGAAGT+AGG + chr1.3:54424025-54424044 MS.gene85551:CDS 35.0%
! GTTGATTCACTTGTGAATGA+TGG + chr1.3:54425597-54425616 MS.gene85551:CDS 35.0%
! TTTTATTGAGTTCATTGCCG+CGG - chr1.3:54425488-54425507 None:intergenic 35.0%
!! CACATGGTATTTTTGAATGC+TGG + chr1.3:54424743-54424762 MS.gene85551:CDS 35.0%
!! CTTGTCAAACTGGTTTGATT+AGG + chr1.3:54423894-54423913 MS.gene85551:CDS 35.0%
AACAAAGCAGCAACCATAAG+AGG - chr1.3:54424106-54424125 None:intergenic 40.0%
AACACAAACCAGCAACTTTG+GGG + chr1.3:54425264-54425283 MS.gene85551:CDS 40.0%
AGAACACAAACCAGCAACTT+TGG + chr1.3:54425262-54425281 MS.gene85551:CDS 40.0%
ATACAGAACAAGGCTTTGCA+AGG + chr1.3:54424780-54424799 MS.gene85551:CDS 40.0%
ATCCTGCTCAATACAGAACA+AGG + chr1.3:54424770-54424789 MS.gene85551:CDS 40.0%
ATCTTCTTCTTGGCATCTGA+AGG - chr1.3:54425077-54425096 None:intergenic 40.0%
ATGCAGCGACTTGATTATCT+CGG + chr1.3:54424315-54424334 MS.gene85551:CDS 40.0%
CAGAAGTAGCACTTCAACAA+TGG + chr1.3:54425521-54425540 MS.gene85551:CDS 40.0%
CTAAGTGTATCTTCGGTTCA+AGG + chr1.3:54424341-54424360 MS.gene85551:CDS 40.0%
CTCCTGATGGAAAATTAGAC+TGG + chr1.3:54425457-54425476 MS.gene85551:CDS 40.0%
GAACACAAACCAGCAACTTT+GGG + chr1.3:54425263-54425282 MS.gene85551:CDS 40.0%
GAACGCCATTACATCATCTT+TGG - chr1.3:54423984-54424003 None:intergenic 40.0%
GAACTTGAAAATCGCCTTAC+AGG + chr1.3:54425138-54425157 MS.gene85551:CDS 40.0%
GAAGGTTGGATCACATGAAA+TGG + chr1.3:54424063-54424082 MS.gene85551:CDS 40.0%
GATGCTTGTGAATATGCACA+TGG + chr1.3:54424727-54424746 MS.gene85551:CDS 40.0%
GGCGATTTCCTTAAGGAAAT+AGG + chr1.3:54424418-54424437 MS.gene85551:CDS 40.0%
GTCTTGCATCTCCTTCAAAT+AGG + chr1.3:54425160-54425179 MS.gene85551:CDS 40.0%
GTGAATCAACCCAAGATTCA+TGG - chr1.3:54425587-54425606 None:intergenic 40.0%
TCAAGGAAGAGGATACTGAA+AGG + chr1.3:54424358-54424377 MS.gene85551:CDS 40.0%
TCCCTACCTGACATTAAGAA+TGG + chr1.3:54424514-54424533 MS.gene85551:CDS 40.0%
TCTTCTTCTTGGCATCTGAA+GGG - chr1.3:54425076-54425095 None:intergenic 40.0%
TGTGAATGATGGTCCAACAA+TGG + chr1.3:54425608-54425627 MS.gene85551:CDS 40.0%
TTCACCAAGTGCTTCTTCAA+TGG + chr1.3:54424915-54424934 MS.gene85551:CDS 40.0%
TTCTTAATGTCAGGTAGGGA+TGG - chr1.3:54424514-54424533 None:intergenic 40.0%
TTGTTCTATTGCTTCTGTCG+AGG + chr1.3:54424231-54424250 MS.gene85551:CDS 40.0%
! ATGTTGATGAAGTAGGGTTG+TGG + chr1.3:54424032-54424051 MS.gene85551:CDS 40.0%
! GACCAGTCTAATTTTCCATC+AGG - chr1.3:54425462-54425481 None:intergenic 40.0%
!! CAAAGTGAAGCCTTGTTCAA+GGG + chr1.3:54424573-54424592 MS.gene85551:CDS 40.0%
!! CTTATGGTTGCTGCTTTGTT+TGG + chr1.3:54424106-54424125 MS.gene85551:CDS 40.0%
!! GAATCCATTGAAGAAGCACT+TGG - chr1.3:54424922-54424941 None:intergenic 40.0%
!! GATGATGTTGATGATGACGA+TGG + chr1.3:54424397-54424416 MS.gene85551:CDS 40.0%
!! GCTGCTTTGTTTGGAAGTAT+GGG + chr1.3:54424115-54424134 MS.gene85551:CDS 40.0%
!! TCAAAGTGAAGCCTTGTTCA+AGG + chr1.3:54424572-54424591 MS.gene85551:CDS 40.0%
!! TGCTGCTTTGTTTGGAAGTA+TGG + chr1.3:54424114-54424133 MS.gene85551:CDS 40.0%
AAGAAACCAGATGCACGAGA+AGG + chr1.3:54424803-54424822 MS.gene85551:CDS 45.0%
AAGGAGATGCAAGACCTGTA+AGG - chr1.3:54425155-54425174 None:intergenic 45.0%
AAGTAGGGTTGTGGTATGGA+AGG + chr1.3:54424041-54424060 MS.gene85551:CDS 45.0%
AGAAACCAGATGCACGAGAA+GGG + chr1.3:54424804-54424823 MS.gene85551:CDS 45.0%
ATTCTCCCCAGGATGAACAA+AGG - chr1.3:54424626-54424645 None:intergenic 45.0%
CAAGGGCTTACTCTCATGAT+TGG + chr1.3:54424590-54424609 MS.gene85551:CDS 45.0%
GATGAAGTAGGGTTGTGGTA+TGG + chr1.3:54424037-54424056 MS.gene85551:CDS 45.0%
GTATCTTCGGTTCAAGGAAG+AGG + chr1.3:54424347-54424366 MS.gene85551:CDS 45.0%
GTTAGTTCCGCTGATGCAAA+CGG + chr1.3:54424286-54424305 MS.gene85551:CDS 45.0%
TCAGGTAGGGATGGATCAAT+GGG - chr1.3:54424505-54424524 None:intergenic 45.0%
TCTGTTACCGTTTGCATCAG+CGG - chr1.3:54424296-54424315 None:intergenic 45.0%
TGAAGAGAGGACACCTCTTA+TGG + chr1.3:54424090-54424109 MS.gene85551:CDS 45.0%
TGACGATGGCGATTTCCTTA+AGG + chr1.3:54424411-54424430 MS.gene85551:CDS 45.0%
TGAGAGTAAGCCCTTGAACA+AGG - chr1.3:54424586-54424605 None:intergenic 45.0%
TGCTGCGCTCAATAAAGCTT+TGG - chr1.3:54425365-54425384 None:intergenic 45.0%
TTCTCCCCAGGATGAACAAA+GGG - chr1.3:54424625-54424644 None:intergenic 45.0%
TTCTTGGCATCTGAAGGGTA+GGG - chr1.3:54425071-54425090 None:intergenic 45.0%
TTGTGGTATGGAAGGAAGGT+TGG + chr1.3:54424049-54424068 MS.gene85551:CDS 45.0%
! AAATTAGACTGGTCCATCCG+CGG + chr1.3:54425468-54425487 MS.gene85551:CDS 45.0%
! GAACCGGTAGAAGCATACAA+CGG - chr1.3:54424865-54424884 None:intergenic 45.0%
!! AGCCTTGTTCTGTATTGAGC+AGG - chr1.3:54424775-54424794 None:intergenic 45.0%
!! GCACTTCATTGTGCTGTTTC+TGG + chr1.3:54424208-54424227 MS.gene85551:CDS 45.0%
AACCTTCTGCCTTCTCTGGT+TGG + chr1.3:54425430-54425449 MS.gene85551:CDS 50.0%
AAGGATTGCCCCAAAGTTGC+TGG - chr1.3:54425275-54425294 None:intergenic 50.0%
ACCTTCTGCCTTCTCTGGTT+GGG + chr1.3:54425431-54425450 MS.gene85551:CDS 50.0%
AGAGAAGGCAGAAGGTTCCA+TGG - chr1.3:54425427-54425446 None:intergenic 50.0%
AGGGTTGTGGTATGGAAGGA+AGG + chr1.3:54424045-54424064 MS.gene85551:CDS 50.0%
AGGTGAAGGTAGAGCAGAAC+CGG - chr1.3:54424881-54424900 None:intergenic 50.0%
AGTGCCCCTTTGTTCATCCT+GGG + chr1.3:54424617-54424636 MS.gene85551:CDS 50.0%
ATCTGCAGCATCATCGCCTA+CGG + chr1.3:54425002-54425021 MS.gene85551:CDS 50.0%
ATGATCCCGTCCGAAACTCA+GGG - chr1.3:54424696-54424715 None:intergenic 50.0%
ATGGAACCTTCTGCCTTCTC+TGG + chr1.3:54425426-54425445 MS.gene85551:CDS 50.0%
ATGGCAACTGAGCTAGCAGA+AGG - chr1.3:54425408-54425427 None:intergenic 50.0%
ATTGAGTTCATTGCCGCGGA+TGG - chr1.3:54425484-54425503 None:intergenic 50.0%
CGACCGTTGTATGCTTCTAC+CGG + chr1.3:54424859-54424878 MS.gene85551:CDS 50.0%
CTGAATCTGAGTCTGCCACA+TGG - chr1.3:54425034-54425053 None:intergenic 50.0%
CTTCTTGGCATCTGAAGGGT+AGG - chr1.3:54425072-54425091 None:intergenic 50.0%
GATGCTGCAGATGGAGTCAA+AGG - chr1.3:54424994-54425013 None:intergenic 50.0%
GTCAGGTAGGGATGGATCAA+TGG - chr1.3:54424506-54424525 None:intergenic 50.0%
GTGGAGCAGCAACAAGATGT+AGG + chr1.3:54424451-54424470 MS.gene85551:CDS 50.0%
GTTGATGTTAACCGAGCGTG+CGG + chr1.3:54424169-54424188 MS.gene85551:CDS 50.0%
TCGGTTAACATCAACGCGAC+CGG - chr1.3:54424164-54424183 None:intergenic 50.0%
TCTCCCCAGGATGAACAAAG+GGG - chr1.3:54424624-54424643 None:intergenic 50.0%
TTCATCCTGGGGAGAATGCA+AGG + chr1.3:54424629-54424648 MS.gene85551:CDS 50.0%
TTCTGCTAGCTCAGTTGCCA+TGG + chr1.3:54425407-54425426 MS.gene85551:CDS 50.0%
!! CTTTTTCGCTCACAAGCCAG+AGG + chr1.3:54424831-54424850 MS.gene85551:CDS 50.0%
CATGATCCCGTCCGAAACTC+AGG - chr1.3:54424697-54424716 None:intergenic 55.0%
CCTTCTGCCTTCTCTGGTTG+GGG + chr1.3:54425432-54425451 MS.gene85551:CDS 55.0%
CGGACGGGATCATGCAACAA+AGG + chr1.3:54424703-54424722 MS.gene85551:CDS 55.0%
GAGTGCCCCTTTGTTCATCC+TGG + chr1.3:54424616-54424635 MS.gene85551:CDS 55.0%
GCAAACCCTTCTCGTGCATC+TGG - chr1.3:54424812-54424831 None:intergenic 55.0%
GCTGCAGATGGAGTCAAAGG+TGG - chr1.3:54424991-54425010 None:intergenic 55.0%
GTAGGCGATGATGCTGCAGA+TGG - chr1.3:54425003-54425022 None:intergenic 55.0%
GTCTCCCTTGCCCTGAGTTT+CGG + chr1.3:54424683-54424702 MS.gene85551:CDS 55.0%
GTGCCCCTTTGTTCATCCTG+GGG + chr1.3:54424618-54424637 MS.gene85551:CDS 55.0%
TACAACGGTCGAAGCTCCTC+TGG - chr1.3:54424850-54424869 None:intergenic 55.0%
!! GCTTTGGCTCTGCTTCGAAG+AGG - chr1.3:54425349-54425368 None:intergenic 55.0%
AGGAGAGCCCCAACCAGAGA+AGG - chr1.3:54425442-54425461 None:intergenic 60.0%
CCCCAACCAGAGAAGGCAGA+AGG - chr1.3:54425435-54425454 None:intergenic 60.0%
CCCTTGCCCTGAGTTTCGGA+CGG + chr1.3:54424687-54424706 MS.gene85551:CDS 60.0%
CCGTCCGAAACTCAGGGCAA+GGG - chr1.3:54424690-54424709 None:intergenic 60.0%
CCTTGCCCTGAGTTTCGGAC+GGG + chr1.3:54424688-54424707 MS.gene85551:CDS 60.0%
TCTGGTTGGGGCTCTCCTGA+TGG + chr1.3:54425444-54425463 MS.gene85551:CDS 60.0%
TGCCACATGGTTCCAGCCGT+AGG - chr1.3:54425021-54425040 None:intergenic 60.0%
ACCGAGCGTGCGGCTCAGAT+AGG + chr1.3:54424179-54424198 MS.gene85551:CDS 65.0%
CCCGTCCGAAACTCAGGGCA+AGG - chr1.3:54424691-54424710 None:intergenic 65.0%
CCCTATCTGAGCCGCACGCT+CGG - chr1.3:54424183-54424202 None:intergenic 65.0%
CCGAGCGTGCGGCTCAGATA+GGG + chr1.3:54424180-54424199 MS.gene85551:CDS 65.0%
CGCCTACGGCTGGAACCATG+TGG + chr1.3:54425016-54425035 MS.gene85551:CDS 65.0%
GCAGCATCATCGCCTACGGC+TGG + chr1.3:54425006-54425025 MS.gene85551:CDS 65.0%
!! ATGCAAGGCGCCGTGATCCG+AGG + chr1.3:54424644-54424663 MS.gene85551:CDS 65.0%
CGGCGCCTTGCATTCTCCCC+AGG - chr1.3:54424637-54424656 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 54423869 54425647 54423869 ID=MS.gene85551
chr1.3 mRNA 54423869 54425647 54423869 ID=MS.gene85551.t1;Parent=MS.gene85551
chr1.3 exon 54423869 54425647 54423869 ID=MS.gene85551.t1.exon1;Parent=MS.gene85551.t1
chr1.3 CDS 54423869 54425647 54423869 ID=cds.MS.gene85551.t1;Parent=MS.gene85551.t1
Gene Sequence

>MS.gene85551

ATGTGCAACGGTTCAAATAGAAAACCTTGTCAAACTGGTTTGATTAGGGAAAATGAATGTGAAAGACATGAAGAACTTCATCATAAGATTTCAGCATTGCTTGAGTTTTCAGCCAAAGATGATGTAATGGCGTTCACAGATGCTGTCGAAAAAGATGATCATGATGTTGATGAAGTAGGGTTGTGGTATGGAAGGAAGGTTGGATCACATGAAATGGGATATGAAGAGAGGACACCTCTTATGGTTGCTGCTTTGTTTGGAAGTATGGGTGTGTTGTCTTATATTCTTGGAACCGGTCGCGTTGATGTTAACCGAGCGTGCGGCTCAGATAGGGCTACTGCACTTCATTGTGCTGTTTCTGGTTGTTCTATTGCTTCTGTCGAGGTTGTCAAGCTTTTGCTTGATGCATCTGCAGATGTTAGTTCCGCTGATGCAAACGGTAACAGATGCAGCGACTTGATTATCTCGGTTTCTAAGTGTATCTTCGGTTCAAGGAAGAGGATACTGAAAGGCTTACTAGAAGGTGTAGATGATGTTGATGATGACGATGGCGATTTCCTTAAGGAAATAGGTTTCAAAATGGTGGAGCAGCAACAAGATGTAGGTACACCTCGAACTGAGAAGAAAGATTATCCCATTGATCCATCCCTACCTGACATTAAGAATGGAATATATAGTACAGATGAATTTAGGATGTTTACATTCAAAGTGAAGCCTTGTTCAAGGGCTTACTCTCATGATTGGACTGAGTGCCCCTTTGTTCATCCTGGGGAGAATGCAAGGCGCCGTGATCCGAGGAAATATCATTACACATGTCTCCCTTGCCCTGAGTTTCGGACGGGATCATGCAACAAAGGAGATGCTTGTGAATATGCACATGGTATTTTTGAATGCTGGCTTCATCCTGCTCAATACAGAACAAGGCTTTGCAAGGAAGAAACCAGATGCACGAGAAGGGTTTGCTTTTTCGCTCACAAGCCAGAGGAGCTTCGACCGTTGTATGCTTCTACCGGTTCTGCTCTACCTTCACCTACATCATATTCTAATTCACCAAGTGCTTCTTCAATGGATTCTTTCACATTGAGCTCCCCGTCTTCTATGATACAATCTGTGTCGACGCCACCTTTGACTCCATCTGCAGCATCATCGCCTACGGCTGGAACCATGTGGCAGACTCAGATTCAGCTTCATGCTGCAGTCCCTACCCTTCAGATGCCAAGAAGAAGATTTAAAACTGCAACGAATGCTAGAAATAATGCTGAATTTCTCGAACTTGAAAATCGCCTTACAGGTCTTGCATCTCCTTCAAATAGGTTTGCAGAAGTGAATCCTACTAACCATGAAAACATTTTCGGGTCATCAATACAATCTACGACATCAATACGAGTGCATCAGAACACAAACCAGCAACTTTGGGGCAATCCTTTTGATCTTACCAATTCAAATGTCATTGGATCATCACAATTTAGAGTTGATGCCTCTTCGAAGCAGAGCCAAAGCTTTATTGAGCGCAGCAGCGTTGCGAGCTTTAACTCTCCTTCTGCTAGCTCAGTTGCCATGGAACCTTCTGCCTTCTCTGGTTGGGGCTCTCCTGATGGAAAATTAGACTGGTCCATCCGCGGCAATGAACTCAATAAAATGAGGAAATCTTACAGAAGTAGCACTTCAACAATGGCTGCTGCATTAAGTGTTAATGATCCAGATATATTTTTAAGCCATGAATCTTGGGTTGATTCACTTGTGAATGATGGTCCAACAATGGAATTTGATCAACATTGA
Protein sequence

>MS.gene85551.t1

MCNGSNRKPCQTGLIRENECERHEELHHKISALLEFSAKDDVMAFTDAVEKDDHDVDEVGLWYGRKVGSHEMGYEERTPLMVAALFGSMGVLSYILGTGRVDVNRACGSDRATALHCAVSGCSIASVEVVKLLLDASADVSSADANGNRCSDLIISVSKCIFGSRKRILKGLLEGVDDVDDDDGDFLKEIGFKMVEQQQDVGTPRTEKKDYPIDPSLPDIKNGIYSTDEFRMFTFKVKPCSRAYSHDWTECPFVHPGENARRRDPRKYHYTCLPCPEFRTGSCNKGDACEYAHGIFECWLHPAQYRTRLCKEETRCTRRVCFFAHKPEELRPLYASTGSALPSPTSYSNSPSASSMDSFTLSSPSSMIQSVSTPPLTPSAASSPTAGTMWQTQIQLHAAVPTLQMPRRRFKTATNARNNAEFLELENRLTGLASPSNRFAEVNPTNHENIFGSSIQSTTSIRVHQNTNQQLWGNPFDLTNSNVIGSSQFRVDASSKQSQSFIERSSVASFNSPSASSVAMEPSAFSGWGSPDGKLDWSIRGNELNKMRKSYRSSTSTMAAALSVNDPDIFLSHESWVDSLVNDGPTMEFDQH