AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85615

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85615.t1 MTR_2g039880 95.563 586 24 1 1 586 1 584 0.0 1102
MS.gene85615.t1 MTR_2g028950 45.747 529 260 10 48 558 45 564 4.67e-146 434
MS.gene85615.t1 MTR_5g090770 40.525 533 291 9 48 563 48 571 3.18e-125 381
MS.gene85615.t1 MTR_2g028950 45.376 465 228 9 48 494 45 501 1.30e-123 374
MS.gene85615.t1 MTR_5g090770 41.951 410 215 7 48 442 48 449 2.78e-97 304
MS.gene85615.t1 MTR_1g051025 31.452 372 211 9 67 402 24 387 3.67e-39 150
MS.gene85615.t1 MTR_7g090020 30.914 372 214 7 67 402 24 388 2.41e-38 147
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85615.t1 AT1G64280 51.839 571 242 10 7 558 7 563 0.0 553
MS.gene85615.t1 AT4G26120 50.261 575 252 11 10 569 17 572 2.49e-176 512
MS.gene85615.t1 AT4G26120 50.261 575 252 11 10 569 18 573 2.62e-176 512
MS.gene85615.t1 AT5G45110 42.830 530 271 11 48 556 41 559 4.31e-124 378
MS.gene85615.t1 AT4G19660 42.481 532 267 11 48 555 35 551 9.35e-124 377
MS.gene85615.t1 AT4G19660 40.608 559 266 12 48 555 35 578 1.48e-118 364
MS.gene85615.t1 AT5G45110 42.062 485 249 11 48 514 41 511 6.75e-110 339
MS.gene85615.t1 AT3G57130 31.522 368 213 7 67 402 26 386 6.06e-41 155
MS.gene85615.t1 AT2G41370 30.053 376 216 8 67 402 25 393 5.71e-37 144

Find 104 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTGAATTTACTCCAAATT+TGG 0.071235 2.1:-48521750 MS.gene85615:CDS
AGCGAGGAGTAGTTTCTTTA+AGG 0.220140 2.1:-48523271 MS.gene85615:CDS
ACTAAACATTCTTGATAAAA+TGG 0.238573 2.1:-48522701 MS.gene85615:CDS
CTCTGGTAACCACCCAAATT+TGG 0.244302 2.1:+48521737 None:intergenic
CACCTTTCAAATTTCTGAAT+TGG 0.266956 2.1:-48523019 MS.gene85615:CDS
GCGCTTCACTTGTCGATCTT+CGG 0.290085 2.1:+48521259 None:intergenic
TGAATTGGTTGCTCTTTATC+AGG 0.291540 2.1:-48523004 MS.gene85615:intron
TATATTACTTGTTTCACTAT+AGG 0.308533 2.1:+48522541 None:intergenic
ATGTTTGTCTAGAAGATTTA+CGG 0.318420 2.1:+48522483 None:intergenic
ATCTAAAGCTCTATGTATTC+TGG 0.325893 2.1:+48522456 None:intergenic
CGCTTCACTTGTCGATCTTC+GGG 0.329375 2.1:+48521260 None:intergenic
GCTACAAGAAGTTCTTAATA+AGG 0.338445 2.1:-48521222 MS.gene85615:CDS
TCTGAATTTACTCCAAATTT+GGG 0.345179 2.1:-48521749 MS.gene85615:CDS
ACTACCGAGCTTCTTGATCT+TGG 0.358468 2.1:-48522328 MS.gene85615:CDS
TCGATTGTGTATCGAGATAT+TGG 0.362910 2.1:-48522086 MS.gene85615:CDS
AATTCAGATGTGCCATCAAT+TGG 0.381830 2.1:+48521764 None:intergenic
AAAGAGGAGTTTGATAAATC+TGG 0.386748 2.1:-48521185 MS.gene85615:CDS
GAAGTAGTAATAGTTGAAAT+TGG 0.386953 2.1:+48523444 None:intergenic
TCATCTTGAGCAGTGGAGTT+GGG 0.392046 2.1:-48521374 MS.gene85615:intron
ATTGTTTACACTTTGGTTGT+TGG 0.395598 2.1:-48523083 MS.gene85615:CDS
GTTCTGCGTTATTTGTATAG+TGG 0.398609 2.1:-48523162 MS.gene85615:CDS
TCATCCTCATCCACGTCAAT+AGG 0.401640 2.1:-48521152 MS.gene85615:CDS
GTGGCTAAGGATTATGATGT+TGG 0.417096 2.1:-48523204 MS.gene85615:CDS
GCTTCAATACTAGATGATAC+AGG 0.421643 2.1:-48523369 MS.gene85615:CDS
AGAAACTTCTCGACCATCTC+TGG 0.426255 2.1:+48523314 None:intergenic
TAAAAGAGGAGCATCTAATT+CGG 0.429046 2.1:-48521679 MS.gene85615:CDS
TCTCTTTCTCTTGCTAAGGC+AGG 0.429539 2.1:-48522025 MS.gene85615:CDS
AACAAAGATACAATAATCTT+CGG 0.437878 2.1:+48522235 None:intergenic
CAGATGATGTAGAATTAGTA+AGG 0.438766 2.1:-48522432 MS.gene85615:CDS
CTCTTTCTCTTGCTAAGGCA+GGG 0.442710 2.1:-48522024 MS.gene85615:CDS
TTCTGCGTTATTTGTATAGT+GGG 0.448053 2.1:-48523161 MS.gene85615:CDS
GTCATCTTGAGCAGTGGAGT+TGG 0.451507 2.1:-48521375 MS.gene85615:intron
ATCATACTATTATTAGCCCT+TGG 0.460763 2.1:+48521125 None:intergenic
GGCATCTCTTTCTCTTGCTA+AGG 0.467473 2.1:-48522029 MS.gene85615:CDS
CCTATCGCAGCTTGCATGCA+TGG 0.468067 2.1:-48521294 MS.gene85615:CDS
TGGTTACCAGAGGACAACTA+TGG 0.474344 2.1:-48521726 MS.gene85615:CDS
ATGGATATCAATCCAATTGA+TGG 0.475189 2.1:-48521776 MS.gene85615:CDS
TGGCTAAGGATTATGATGTT+GGG 0.476464 2.1:-48523203 MS.gene85615:CDS
CTTTCTATAGCAAACGTTTG+TGG 0.477691 2.1:-48522655 MS.gene85615:CDS
ACGGTTCTTCCCTCGTTGCT+CGG 0.483600 2.1:-48521348 MS.gene85615:CDS
TGAAGCATGAAATCAACAAC+AGG 0.485589 2.1:+48523060 None:intergenic
TTCAGAAATTTGAAAGGTGA+AGG 0.491260 2.1:+48523023 None:intergenic
ATCACAACCTTTGCTTCCAT+CGG 0.509584 2.1:+48521797 None:intergenic
GAGCAGTGGAGTTGGGGAAA+CGG 0.514130 2.1:-48521367 MS.gene85615:intron
TCTTTCTCTTGCTAAGGCAG+GGG 0.519191 2.1:-48522023 MS.gene85615:CDS
ATTGGAGCAAGCTGAGAGAA+GGG 0.524671 2.1:-48522068 MS.gene85615:CDS
GATGATGATTGTTTACACTT+TGG 0.524715 2.1:-48523090 MS.gene85615:CDS
GTCGAGAAGTTTCTGTGCAC+CGG 0.525175 2.1:-48523305 MS.gene85615:CDS
TCAAGTCCATAGTTGTCCTC+TGG 0.525929 2.1:+48521720 None:intergenic
GGAAGTGTTGAACAAGATCA+TGG 0.534097 2.1:-48521327 MS.gene85615:CDS
ATTGTATCTTTGTTAACGAA+GGG 0.535112 2.1:-48522226 MS.gene85615:CDS
GTAATGATGTACTTGAGAAG+TGG 0.540852 2.1:-48523537 MS.gene85615:CDS
TATTGGAGCAAGCTGAGAGA+AGG 0.543360 2.1:-48522069 MS.gene85615:CDS
TATTATAAATCTACTGAGGA+AGG 0.543578 2.1:-48522127 MS.gene85615:CDS
ATGAAGGCGTTGTCTAGAGC+AGG 0.543907 2.1:-48521656 MS.gene85615:intron
CATTCCTATTGACGTGGATG+AGG 0.544059 2.1:+48521148 None:intergenic
ACTTGTTTCACTATAGGTTG+AGG 0.548262 2.1:+48522547 None:intergenic
TAAATCTACTGAGGAAGGAA+AGG 0.549862 2.1:-48522122 MS.gene85615:CDS
GGCACCATTTAAAATAAAAG+AGG 0.551112 2.1:-48521693 MS.gene85615:CDS
CCTTCTGAACTCACTTTCGA+TGG 0.555532 2.1:-48522196 MS.gene85615:CDS
AAGATCAAGAAGCTCGGTAG+TGG 0.558220 2.1:+48522330 None:intergenic
GGAGCATCTAATTCGGATGA+AGG 0.559886 2.1:-48521672 MS.gene85615:CDS
CATCTTGAGCAGTGGAGTTG+GGG 0.560586 2.1:-48521373 MS.gene85615:intron
CTATCGCAGCTTGCATGCAT+GGG 0.560772 2.1:-48521293 MS.gene85615:CDS
GCAACTGAAGAAAGTGGCTA+AGG 0.563471 2.1:-48523217 MS.gene85615:CDS
AACCAATTCAGAAATTTGAA+AGG 0.567258 2.1:+48523017 None:intergenic
CCATGCATGCAAGCTGCGAT+AGG 0.568643 2.1:+48521294 None:intergenic
AGAGATGCCTCACCATGTAA+AGG 0.569360 2.1:+48522043 None:intergenic
GAGAGAAGGGAGCCTTTACA+TGG 0.569857 2.1:-48522055 MS.gene85615:CDS
TATTGTATCTTTGTTAACGA+AGG 0.570454 2.1:-48522227 MS.gene85615:CDS
GCAAAGATCATTGCCAGAGA+TGG 0.570514 2.1:-48523327 MS.gene85615:CDS
CCATCGAAAGTGAGTTCAGA+AGG 0.573318 2.1:+48522196 None:intergenic
TAAAGATGTTTGTGTTTGTG+TGG 0.576566 2.1:-48523112 MS.gene85615:CDS
TTTGTTTCCGATGGAAGCAA+AGG 0.576921 2.1:-48521804 MS.gene85615:CDS
CTTCAATACTAGATGATACA+GGG 0.581620 2.1:-48523368 MS.gene85615:CDS
GACGCTGCAACTGAAGAAAG+TGG 0.581731 2.1:-48523223 MS.gene85615:CDS
TTCTCTTCGCAGCAACATGT+AGG 0.583462 2.1:+48522263 None:intergenic
GACAACTATGGACTTGAATG+AGG 0.586182 2.1:-48521714 MS.gene85615:CDS
TCTTAATAAGGTCTTTCATG+AGG 0.586342 2.1:-48521210 MS.gene85615:CDS
GAATTTACTCCAAATTTGGG+TGG 0.590945 2.1:-48521746 MS.gene85615:CDS
GATGGAAGCAAAGGTTGTGA+TGG 0.592814 2.1:-48521795 MS.gene85615:CDS
CCTTGGCATTCCTATTGACG+TGG 0.593232 2.1:+48521142 None:intergenic
CCACGTCAATAGGAATGCCA+AGG 0.605123 2.1:-48521142 MS.gene85615:CDS
ACAGCATAACTCACTAGCAC+TGG 0.609316 2.1:+48523476 None:intergenic
AAGCTAGATTGTCTGAGAGA+CGG 0.610515 2.1:+48523388 None:intergenic
TCCTCGCTGACAGAATGCAC+CGG 0.615150 2.1:+48523286 None:intergenic
AAAGCGTAGTACGAAAGCTG+TGG 0.616351 2.1:-48522152 MS.gene85615:CDS
AAGGGAGCCTTTACATGGTG+AGG 0.619314 2.1:-48522050 MS.gene85615:CDS
GGTCTTTCATGAGGACAAAG+AGG 0.622648 2.1:-48521201 MS.gene85615:CDS
GGCAATAGATGACATGTTGG+TGG 0.623200 2.1:-48522680 MS.gene85615:CDS
AATGGCAATAGATGACATGT+TGG 0.633106 2.1:-48522683 MS.gene85615:CDS
CATGTGATAAACTACTAACA+AGG 0.637446 2.1:-48522627 MS.gene85615:CDS
AGTGAAGCGCAGACGATACG+CGG 0.639553 2.1:-48521246 MS.gene85615:CDS
AAGTCCAAGATCAAGAAGCT+CGG 0.641506 2.1:+48522324 None:intergenic
ATATACAAACGTAAACAACT+AGG 0.645283 2.1:-48522523 MS.gene85615:CDS
CAAATTTGGGTGGTTACCAG+AGG 0.650574 2.1:-48521736 MS.gene85615:CDS
CACGTCAATAGGAATGCCAA+GGG 0.650742 2.1:-48521141 MS.gene85615:CDS
AGGACACACTACTCTAGATG+AGG 0.655016 2.1:-48522395 MS.gene85615:CDS
ACCGGTGCATTCTGTCAGCG+AGG 0.681670 2.1:-48523287 MS.gene85615:CDS
TTGTCTGAGAGACGGTTGAG+TGG 0.683526 2.1:+48523396 None:intergenic
TCAACACTTCCGAGCAACGA+GGG 0.684586 2.1:+48521339 None:intergenic
TTCAACACTTCCGAGCAACG+AGG 0.701823 2.1:+48521338 None:intergenic
GGATTATTATAAATCTACTG+AGG 0.714422 2.1:-48522131 MS.gene85615:CDS
TTGTTGTACCTAGAAAACCG+AGG 0.809311 2.1:-48521983 MS.gene85615:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTTAAAGTAATTTCATA+GGG - chr2.1:48521710-48521729 MS.gene85615:CDS 15.0%
!! ATCTAACTTATAATGAAAAT+TGG + chr2.1:48523185-48523204 None:intergenic 15.0%
!! ATGAAATTAATTTAACTGAA+AGG + chr2.1:48521856-48521875 None:intergenic 15.0%
!! ATGTAAATCATAAAAATCTT+AGG - chr2.1:48522346-48522365 MS.gene85615:CDS 15.0%
!! TCAAAAATAAAATGAAAGAT+GGG + chr2.1:48523029-48523048 None:intergenic 15.0%
!! TGCAAAATTTAACAAATAAA+TGG + chr2.1:48523216-48523235 None:intergenic 15.0%
!! TGTAAATCATAAAAATCTTA+GGG - chr2.1:48522347-48522366 MS.gene85615:CDS 15.0%
!!! AAGATTTTTATGATTTACAT+CGG + chr2.1:48522346-48522365 None:intergenic 15.0%
!!! CATTTTTGATTTATATACAA+TGG + chr2.1:48523094-48523113 None:intergenic 15.0%
!! AACAAAGATACAATAATCTT+CGG + chr2.1:48522411-48522430 None:intergenic 20.0%
!! AAGTTAAAGTAATTTCATAG+GGG - chr2.1:48521711-48521730 MS.gene85615:CDS 20.0%
!! ACTAAACATTCTTGATAAAA+TGG - chr2.1:48521942-48521961 MS.gene85615:intron 20.0%
!! CTCAAAAATAAAATGAAAGA+TGG + chr2.1:48523030-48523049 None:intergenic 20.0%
!! GAAAGTTAAAGTAATTTCAT+AGG - chr2.1:48521709-48521728 MS.gene85615:CDS 20.0%
!! GTAAAGAAAGAAAAATAGAA+TGG + chr2.1:48523120-48523139 None:intergenic 20.0%
!! GTAAATCATAAAAATCTTAG+GGG - chr2.1:48522348-48522367 MS.gene85615:CDS 20.0%
!! TATATTACTTGTTTCACTAT+AGG + chr2.1:48522105-48522124 None:intergenic 20.0%
!! TCAACATTTATTGTGAAATT+CGG + chr2.1:48522777-48522796 None:intergenic 20.0%
!!! ATTTTTGAGTACTTTCTTAA+AGG - chr2.1:48523041-48523060 MS.gene85615:CDS 20.0%
!!! TTCTTTAAGGATGTTTTTAA+GGG - chr2.1:48521385-48521404 MS.gene85615:intron 20.0%
!!! TTTCTTTAAGGATGTTTTTA+AGG - chr2.1:48521384-48521403 MS.gene85615:intron 20.0%
! AACCAATTCAGAAATTTGAA+AGG + chr2.1:48521629-48521648 None:intergenic 25.0%
! ATATACAAACGTAAACAACT+AGG - chr2.1:48522120-48522139 MS.gene85615:CDS 25.0%
! ATCTGAATTTACTCCAAATT+TGG - chr2.1:48522893-48522912 MS.gene85615:intron 25.0%
! ATGTTTGTCTAGAAGATTTA+CGG + chr2.1:48522163-48522182 None:intergenic 25.0%
! ATTGTATCTTTGTTAACGAA+GGG - chr2.1:48522417-48522436 MS.gene85615:CDS 25.0%
! CAACATTTATTGTGAAATTC+GGG + chr2.1:48522776-48522795 None:intergenic 25.0%
! GAAGTAGTAATAGTTGAAAT+TGG + chr2.1:48521202-48521221 None:intergenic 25.0%
! GGATTATTATAAATCTACTG+AGG - chr2.1:48522512-48522531 MS.gene85615:CDS 25.0%
! GTTCGAAAACAATTGATATA+GGG - chr2.1:48523158-48523177 MS.gene85615:CDS 25.0%
! TAATTTAACTGAAAGGTCAT+AGG + chr2.1:48521849-48521868 None:intergenic 25.0%
! TATTATAAATCTACTGAGGA+AGG - chr2.1:48522516-48522535 MS.gene85615:CDS 25.0%
! TATTGTATCTTTGTTAACGA+AGG - chr2.1:48522416-48522435 MS.gene85615:CDS 25.0%
! TCTGAATTTACTCCAAATTT+GGG - chr2.1:48522894-48522913 MS.gene85615:intron 25.0%
! TTTCTTTACTTGTATCTATC+TGG - chr2.1:48523131-48523150 MS.gene85615:CDS 25.0%
!!! TGCTCCTCTTTTATTTTAAA+TGG + chr2.1:48522957-48522976 None:intergenic 25.0%
AAAGAGGAGTTTGATAAATC+TGG - chr2.1:48523458-48523477 MS.gene85615:CDS 30.0%
ATCATACTATTATTAGCCCT+TGG + chr2.1:48523521-48523540 None:intergenic 30.0%
ATGGATATCAATCCAATTGA+TGG - chr2.1:48522867-48522886 MS.gene85615:intron 30.0%
ATTGTTTACACTTTGGTTGT+TGG - chr2.1:48521560-48521579 MS.gene85615:intron 30.0%
CACCTTTCAAATTTCTGAAT+TGG - chr2.1:48521624-48521643 MS.gene85615:intron 30.0%
CAGATGATGTAGAATTAGTA+AGG - chr2.1:48522211-48522230 MS.gene85615:CDS 30.0%
CATGTGATAAACTACTAACA+AGG - chr2.1:48522016-48522035 MS.gene85615:CDS 30.0%
CTTCAATACTAGATGATACA+GGG - chr2.1:48521275-48521294 MS.gene85615:CDS 30.0%
GAACATGTAAGAAAATACCT+CGG + chr2.1:48522680-48522699 None:intergenic 30.0%
GATGATGATTGTTTACACTT+TGG - chr2.1:48521553-48521572 MS.gene85615:intron 30.0%
GCTACAAGAAGTTCTTAATA+AGG - chr2.1:48523421-48523440 MS.gene85615:CDS 30.0%
GGTTCGAAAACAATTGATAT+AGG - chr2.1:48523157-48523176 MS.gene85615:CDS 30.0%
TAAAGATGTTTGTGTTTGTG+TGG - chr2.1:48521531-48521550 MS.gene85615:intron 30.0%
TCTTAATAAGGTCTTTCATG+AGG - chr2.1:48523433-48523452 MS.gene85615:CDS 30.0%
TTACTTGTATCTATCTGGTT+CGG - chr2.1:48523136-48523155 MS.gene85615:CDS 30.0%
TTCAGAAATTTGAAAGGTGA+AGG + chr2.1:48521623-48521642 None:intergenic 30.0%
TTCTGCGTTATTTGTATAGT+GGG - chr2.1:48521482-48521501 MS.gene85615:intron 30.0%
! ATCTAAAGCTCTATGTATTC+TGG + chr2.1:48522190-48522209 None:intergenic 30.0%
! GGCACCATTTAAAATAAAAG+AGG - chr2.1:48522950-48522969 MS.gene85615:intron 30.0%
!! ACGATTCATTGTTTTTCAGT+TGG - chr2.1:48522804-48522823 MS.gene85615:intron 30.0%
!! TAAAAGAGGAGCATCTAATT+CGG - chr2.1:48522964-48522983 MS.gene85615:intron 30.0%
!! TAGTTGATTTGATTACTTGC+AGG - chr2.1:48521912-48521931 MS.gene85615:intron 30.0%
AATGGCAATAGATGACATGT+TGG - chr2.1:48521960-48521979 MS.gene85615:intron 35.0%
AATTCAGATGTGCCATCAAT+TGG + chr2.1:48522882-48522901 None:intergenic 35.0%
ACTTGTTTCACTATAGGTTG+AGG + chr2.1:48522099-48522118 None:intergenic 35.0%
AGCCTCTTGTGTAAAAAACA+GGG + chr2.1:48521810-48521829 None:intergenic 35.0%
AGTACTTTCTTAAAGGTCGA+AGG - chr2.1:48523048-48523067 MS.gene85615:CDS 35.0%
CTTTCTATAGCAAACGTTTG+TGG - chr2.1:48521988-48522007 MS.gene85615:CDS 35.0%
GAATTTACTCCAAATTTGGG+TGG - chr2.1:48522897-48522916 MS.gene85615:intron 35.0%
GCTTCAATACTAGATGATAC+AGG - chr2.1:48521274-48521293 MS.gene85615:CDS 35.0%
GTTCTGCGTTATTTGTATAG+TGG - chr2.1:48521481-48521500 MS.gene85615:intron 35.0%
TAAATCTACTGAGGAAGGAA+AGG - chr2.1:48522521-48522540 MS.gene85615:CDS 35.0%
TCGATTGTGTATCGAGATAT+TGG - chr2.1:48522557-48522576 MS.gene85615:CDS 35.0%
TGAAGCATGAAATCAACAAC+AGG + chr2.1:48521586-48521605 None:intergenic 35.0%
TGAATTGGTTGCTCTTTATC+AGG - chr2.1:48521639-48521658 MS.gene85615:intron 35.0%
TGGCTAAGGATTATGATGTT+GGG - chr2.1:48521440-48521459 MS.gene85615:intron 35.0%
TTCGGGAGTAACATATGTAA+AGG + chr2.1:48522759-48522778 None:intergenic 35.0%
! AGAAAATACCTCGGTTTTCT+AGG + chr2.1:48522671-48522690 None:intergenic 35.0%
!! AGCAAAACTTTTGTTTCCGA+TGG - chr2.1:48522830-48522849 MS.gene85615:intron 35.0%
!! CCATTTTGCTTTATTGTACG+CGG - chr2.1:48521779-48521798 MS.gene85615:CDS 35.0%
!!! GTAAGGTTGCTTTTGAAAGA+AGG - chr2.1:48522228-48522247 MS.gene85615:CDS 35.0%
!!! TACCCTGTTTTTTACACAAG+AGG - chr2.1:48521805-48521824 MS.gene85615:CDS 35.0%
AAGCTAGATTGTCTGAGAGA+CGG + chr2.1:48521258-48521277 None:intergenic 40.0%
AAGGTCATAGGTTCAAGTCT+CGG + chr2.1:48521837-48521856 None:intergenic 40.0%
AAGTCCAAGATCAAGAAGCT+CGG + chr2.1:48522322-48522341 None:intergenic 40.0%
AGCGAGGAGTAGTTTCTTTA+AGG - chr2.1:48521372-48521391 MS.gene85615:intron 40.0%
ATCACAACCTTTGCTTCCAT+CGG + chr2.1:48522849-48522868 None:intergenic 40.0%
CAGCCTCTTGTGTAAAAAAC+AGG + chr2.1:48521811-48521830 None:intergenic 40.0%
CCGCGTACAATAAAGCAAAA+TGG + chr2.1:48521782-48521801 None:intergenic 40.0%
GACAACTATGGACTTGAATG+AGG - chr2.1:48522929-48522948 MS.gene85615:intron 40.0%
GTGGCTAAGGATTATGATGT+TGG - chr2.1:48521439-48521458 MS.gene85615:intron 40.0%
TTGTTGTACCTAGAAAACCG+AGG - chr2.1:48522660-48522679 MS.gene85615:CDS 40.0%
TTTGTTTCCGATGGAAGCAA+AGG - chr2.1:48522839-48522858 MS.gene85615:intron 40.0%
!! GGAAGTGTTGAACAAGATCA+TGG - chr2.1:48523316-48523335 MS.gene85615:CDS 40.0%
!! TTTTTGGTCATCTTGAGCAG+TGG - chr2.1:48523262-48523281 MS.gene85615:CDS 40.0%
!!! AGGCAGTGATTTTTCAAGAG+TGG + chr2.1:48522079-48522098 None:intergenic 40.0%
AAAGCGTAGTACGAAAGCTG+TGG - chr2.1:48522491-48522510 MS.gene85615:CDS 45.0%
AAGATCAAGAAGCTCGGTAG+TGG + chr2.1:48522316-48522335 None:intergenic 45.0%
ACAGCATAACTCACTAGCAC+TGG + chr2.1:48521170-48521189 None:intergenic 45.0%
ACTACCGAGCTTCTTGATCT+TGG - chr2.1:48522315-48522334 MS.gene85615:CDS 45.0%
AGAAACTTCTCGACCATCTC+TGG + chr2.1:48521332-48521351 None:intergenic 45.0%
AGAGATGCCTCACCATGTAA+AGG + chr2.1:48522603-48522622 None:intergenic 45.0%
AGGACACACTACTCTAGATG+AGG - chr2.1:48522248-48522267 MS.gene85615:CDS 45.0%
ATTGGAGCAAGCTGAGAGAA+GGG - chr2.1:48522575-48522594 MS.gene85615:CDS 45.0%
CAAATTTGGGTGGTTACCAG+AGG - chr2.1:48522907-48522926 MS.gene85615:intron 45.0%
CACGTCAATAGGAATGCCAA+GGG - chr2.1:48523502-48523521 MS.gene85615:CDS 45.0%
CATTCCTATTGACGTGGATG+AGG + chr2.1:48523498-48523517 None:intergenic 45.0%
CCATCGAAAGTGAGTTCAGA+AGG + chr2.1:48522450-48522469 None:intergenic 45.0%
CCTTCTGAACTCACTTTCGA+TGG - chr2.1:48522447-48522466 MS.gene85615:CDS 45.0%
CTCTGGTAACCACCCAAATT+TGG + chr2.1:48522909-48522928 None:intergenic 45.0%
CTCTTTCTCTTGCTAAGGCA+GGG - chr2.1:48522619-48522638 MS.gene85615:CDS 45.0%
GATGGAAGCAAAGGTTGTGA+TGG - chr2.1:48522848-48522867 MS.gene85615:intron 45.0%
GCAAAGATCATTGCCAGAGA+TGG - chr2.1:48521316-48521335 MS.gene85615:CDS 45.0%
GCAACTGAAGAAAGTGGCTA+AGG - chr2.1:48521426-48521445 MS.gene85615:intron 45.0%
GGCAATAGATGACATGTTGG+TGG - chr2.1:48521963-48521982 MS.gene85615:intron 45.0%
GGCATCTCTTTCTCTTGCTA+AGG - chr2.1:48522614-48522633 MS.gene85615:CDS 45.0%
GGTCTTTCATGAGGACAAAG+AGG - chr2.1:48523442-48523461 MS.gene85615:CDS 45.0%
TATTGGAGCAAGCTGAGAGA+AGG - chr2.1:48522574-48522593 MS.gene85615:CDS 45.0%
TCAAGTCCATAGTTGTCCTC+TGG + chr2.1:48522926-48522945 None:intergenic 45.0%
TCATCCTCATCCACGTCAAT+AGG - chr2.1:48523491-48523510 MS.gene85615:CDS 45.0%
TCATCTTGAGCAGTGGAGTT+GGG - chr2.1:48523269-48523288 MS.gene85615:CDS 45.0%
TCTCTTTCTCTTGCTAAGGC+AGG - chr2.1:48522618-48522637 MS.gene85615:CDS 45.0%
TCTTTCTCTTGCTAAGGCAG+GGG - chr2.1:48522620-48522639 MS.gene85615:CDS 45.0%
TGGTTACCAGAGGACAACTA+TGG - chr2.1:48522917-48522936 MS.gene85615:intron 45.0%
TTCTCTTCGCAGCAACATGT+AGG + chr2.1:48522383-48522402 None:intergenic 45.0%
! GGAGCATCTAATTCGGATGA+AGG - chr2.1:48522971-48522990 MS.gene85615:intron 45.0%
!!! TATATTTTATTTTGATTTTT+TGG - chr2.1:48523246-48523265 MS.gene85615:CDS 5.0%
AAAATGGTGAGACCCCTTGC+AGG + chr2.1:48521766-48521785 None:intergenic 50.0%
AAGGGAGCCTTTACATGGTG+AGG - chr2.1:48522593-48522612 MS.gene85615:CDS 50.0%
CATCTTGAGCAGTGGAGTTG+GGG - chr2.1:48523270-48523289 MS.gene85615:CDS 50.0%
CCACGTCAATAGGAATGCCA+AGG - chr2.1:48523501-48523520 MS.gene85615:CDS 50.0%
CCGTGCACTAAAACTAGGCA+GGG - chr2.1:48521739-48521758 MS.gene85615:CDS 50.0%
CCTTGGCATTCCTATTGACG+TGG + chr2.1:48523504-48523523 None:intergenic 50.0%
CGCTTCACTTGTCGATCTTC+GGG + chr2.1:48523386-48523405 None:intergenic 50.0%
CTATCGCAGCTTGCATGCAT+GGG - chr2.1:48523350-48523369 MS.gene85615:CDS 50.0%
GACGCTGCAACTGAAGAAAG+TGG - chr2.1:48521420-48521439 MS.gene85615:intron 50.0%
GAGAGAAGGGAGCCTTTACA+TGG - chr2.1:48522588-48522607 MS.gene85615:CDS 50.0%
GCGCTTCACTTGTCGATCTT+CGG + chr2.1:48523387-48523406 None:intergenic 50.0%
GTCATCTTGAGCAGTGGAGT+TGG - chr2.1:48523268-48523287 MS.gene85615:CDS 50.0%
GTCGAGAAGTTTCTGTGCAC+CGG - chr2.1:48521338-48521357 MS.gene85615:CDS 50.0%
TCAACACTTCCGAGCAACGA+GGG + chr2.1:48523307-48523326 None:intergenic 50.0%
TTCAACACTTCCGAGCAACG+AGG + chr2.1:48523308-48523327 None:intergenic 50.0%
TTGTCTGAGAGACGGTTGAG+TGG + chr2.1:48521250-48521269 None:intergenic 50.0%
!! ATGAAGGCGTTGTCTAGAGC+AGG - chr2.1:48522987-48523006 MS.gene85615:intron 50.0%
!!! CCCTGCCTAGTTTTAGTGCA+CGG + chr2.1:48521742-48521761 None:intergenic 50.0%
!!! CCTGCCTAGTTTTAGTGCAC+GGG + chr2.1:48521741-48521760 None:intergenic 50.0%
!!! CTGCCTAGTTTTAGTGCACG+GGG + chr2.1:48521740-48521759 None:intergenic 50.0%
AAACTAGGCAGGGTCCTGCA+AGG - chr2.1:48521749-48521768 MS.gene85615:CDS 55.0%
AACTAGGCAGGGTCCTGCAA+GGG - chr2.1:48521750-48521769 MS.gene85615:CDS 55.0%
ACGGTTCTTCCCTCGTTGCT+CGG - chr2.1:48523295-48523314 MS.gene85615:CDS 55.0%
AGTGAAGCGCAGACGATACG+CGG - chr2.1:48523397-48523416 MS.gene85615:CDS 55.0%
CAGCCCCGTGCACTAAAACT+AGG - chr2.1:48521734-48521753 MS.gene85615:CDS 55.0%
CCATGCATGCAAGCTGCGAT+AGG + chr2.1:48523352-48523371 None:intergenic 55.0%
CCCGTGCACTAAAACTAGGC+AGG - chr2.1:48521738-48521757 MS.gene85615:CDS 55.0%
CCTATCGCAGCTTGCATGCA+TGG - chr2.1:48523349-48523368 MS.gene85615:CDS 55.0%
GAGCAGTGGAGTTGGGGAAA+CGG - chr2.1:48523276-48523295 MS.gene85615:CDS 55.0%
TCCTCGCTGACAGAATGCAC+CGG + chr2.1:48521360-48521379 None:intergenic 55.0%
ACCGGTGCATTCTGTCAGCG+AGG - chr2.1:48521356-48521375 MS.gene85615:CDS 60.0%
ACTAGGCAGGGTCCTGCAAG+GGG - chr2.1:48521751-48521770 MS.gene85615:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 48521109 48523556 48521109 ID=MS.gene85615
chr2.1 mRNA 48521109 48523556 48521109 ID=MS.gene85615.t1;Parent=MS.gene85615
chr2.1 exon 48523005 48523556 48523005 ID=MS.gene85615.t1.exon1;Parent=MS.gene85615.t1
chr2.1 CDS 48523005 48523556 48523005 ID=cds.MS.gene85615.t1;Parent=MS.gene85615.t1
chr2.1 exon 48521984 48522731 48521984 ID=MS.gene85615.t1.exon2;Parent=MS.gene85615.t1
chr2.1 CDS 48521984 48522731 48521984 ID=cds.MS.gene85615.t1;Parent=MS.gene85615.t1
chr2.1 exon 48521657 48521842 48521657 ID=MS.gene85615.t1.exon3;Parent=MS.gene85615.t1
chr2.1 CDS 48521657 48521842 48521657 ID=cds.MS.gene85615.t1;Parent=MS.gene85615.t1
chr2.1 exon 48521109 48521383 48521109 ID=MS.gene85615.t1.exon4;Parent=MS.gene85615.t1
chr2.1 CDS 48521109 48521383 48521109 ID=cds.MS.gene85615.t1;Parent=MS.gene85615.t1
Gene Sequence

>MS.gene85615

ATGATGTACTTGAGAAGTGGATTCTCTGAATATTCAAACGAAATCAGCAACAACACTGCCAGTGCTAGTGAGTTATGCTGTACAACAGCACCAATTTCAACTATTACTACTTCACAAGATATCATCACAATCACACAACCACTCAACCGTCTCTCAGACAATCTAGCTTCAATACTAGATGATACAGGGTTTGACTTCTTTTCTGACGCAAAGATCATTGCCAGAGATGGTCGAGAAGTTTCTGTGCACCGGTGCATTCTGTCAGCGAGGAGTAGTTTCTTTAAGGATGTTTTTAAGGGAAAGAAAGAAACGACGCTGCAACTGAAGAAAGTGGCTAAGGATTATGATGTTGGGTTTGAAGCGCTTAACGTTGTTCTGCGTTATTTGTATAGTGGGAGAGTTGATAATCATCATTTGTCTGCTAAAGATGTTTGTGTTTGTGTGGATGATGATTGTTTACACTTTGGTTGTTGGCCTGTTGTTGATTTCATGCTTCAACTTCTCTATGTTTCCTTCACCTTTCAAATTTCTGAATTGGTTGCTCTTTATCAGGTAAGAATTAAGCACTTATAGATGCTATTTTAATAATAAAAGATGAAAGAAAGTTAAAGTAATTTCATAGGGGCAGCCCCGTGCACTAAAACTAGGCAGGGTCCTGCAAGGGGTCTCACCATTTTGCTTTATTGTACGCGGTCTTACCCTGTTTTTTACACAAGAGGCTGTTTCCGAGACTTGAACCTATGACCTTTCAGTTAAATTAATTTCATATAGTTTATTAAAATAATCTTAAAACAATTTCTTGATAGTTGATTTGATTACTTGCAGGATCATCTACTAAACATTCTTGATAAAATGGCAATAGATGACATGTTGGTGGTTCTTTCTATAGCAAACGTTTGTGGTAAAACATGTGATAAACTACTAACAAGGTGTACAGAGATAATAGTTGAATCTAATGTTGATATAACCACTCTTGAAAAATCACTGCCTCAACCTATAGTGAAACAAGTAATATACAAACGTAAACAACTAGGTTTAGACATGTATGAAACCGTAAATCTTCTAGACAAACATGTCACCAGAATACATAGAGCTTTAGATTCAGATGATGTAGAATTAGTAAGGTTGCTTTTGAAAGAAGGACACACTACTCTAGATGAGGCTCATGCACTTCACTATGCTGTTGCTTATTGTGATGTCAAAACCACTACCGAGCTTCTTGATCTTGGACTTGCCGATGTAAATCATAAAAATCTTAGGGGCTACAGCGTCCTACATGTTGCTGCGAAGAGAAAAGAACCGAAGATTATTGTATCTTTGTTAACGAAGGGAGCTCAACCTTCTGAACTCACTTTCGATGGCAGAAAAGCTCTTCAAATTTCAAAGCGTAGTACGAAAGCTGTGGATTATTATAAATCTACTGAGGAAGGAAAGGTTTCTTCGAATGATCGATTGTGTATCGAGATATTGGAGCAAGCTGAGAGAAGGGAGCCTTTACATGGTGAGGCATCTCTTTCTCTTGCTAAGGCAGGGGATGATTTACGTATGAAATTGTTGTACCTAGAAAACCGAGGTATTTTCTTACATGTTCATATTTATCGATTTATTTTCTTTTTTTCGATAAATGTTGAATCAACATTTGTGTCGCCTTTACATATGTTACTCCCGAATTTCACAATAAATGTTGATCAACTGACGATTCATTGTTTTTCAGTTGGACTAGCAAAACTTTTGTTTCCGATGGAAGCAAAGGTTGTGATGGATATCAATCCAATTGATGGCACATCTGAATTTACTCCAAATTTGGGTGGTTACCAGAGGACAACTATGGACTTGAATGAGGCACCATTTAAAATAAAAGAGGAGCATCTAATTCGGATGAAGGCGTTGTCTAGAGCAGGTAATATATACATGTTTCCCATCTTTCATTTTATTTTTGAGTACTTTCTTAAAGGTCGAAGGCTCAAAGTAATTCTTATCAACCATTGTATATAAATCAAAAATGTTTCCATTCTATTTTTCTTTCTTTACTTGTATCTATCTGGTTCGGTTCGAAAACAATTGATATAGGGACCAATTTTCATTATAAGTTAGATTTTAGTCACCATTTATTTGTTAAATTTTGCAAATTGATGCTTATATTTTATTTTGATTTTTTGGTCATCTTGAGCAGTGGAGTTGGGGAAACGGTTCTTCCCTCGTTGCTCGGAAGTGTTGAACAAGATCATGGATGTCGATGACCTATCGCAGCTTGCATGCATGGGTCATGACAGTCCCGAAGATCGACAAGTGAAGCGCAGACGATACGCGGAGCTACAAGAAGTTCTTAATAAGGTCTTTCATGAGGACAAAGAGGAGTTTGATAAATCTGGCATGTCTTCATCATCCTCATCCACGTCAATAGGAATGCCAAGGGCTAATAATAGTATGATAGCAATGAATCATTGA
Protein sequence

>MS.gene85615.t1

MMYLRSGFSEYSNEISNNTASASELCCTTAPISTITTSQDIITITQPLNRLSDNLASILDDTGFDFFSDAKIIARDGREVSVHRCILSARSSFFKDVFKGKKETTLQLKKVAKDYDVGFEALNVVLRYLYSGRVDNHHLSAKDVCVCVDDDCLHFGCWPVVDFMLQLLYVSFTFQISELVALYQDHLLNILDKMAIDDMLVVLSIANVCGKTCDKLLTRCTEIIVESNVDITTLEKSLPQPIVKQVIYKRKQLGLDMYETVNLLDKHVTRIHRALDSDDVELVRLLLKEGHTTLDEAHALHYAVAYCDVKTTTELLDLGLADVNHKNLRGYSVLHVAAKRKEPKIIVSLLTKGAQPSELTFDGRKALQISKRSTKAVDYYKSTEEGKVSSNDRLCIEILEQAERREPLHGEASLSLAKAGDDLRMKLLYLENRVGLAKLLFPMEAKVVMDINPIDGTSEFTPNLGGYQRTTMDLNEAPFKIKEEHLIRMKALSRAVELGKRFFPRCSEVLNKIMDVDDLSQLACMGHDSPEDRQVKRRRYAELQEVLNKVFHEDKEEFDKSGMSSSSSSTSIGMPRANNSMIAMNH