Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85699.t1 | XP_024632961.1 | 88.4 | 311 | 12 | 3 | 1 | 287 | 1 | 311 | 1.60E-135 | 492.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85699.t1 | Q9SZL8 | 27.1 | 255 | 173 | 5 | 42 | 290 | 72 | 319 | 9.4e-20 | 99.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85699.t1 | A0A072TE13 | 83.2 | 303 | 28 | 3 | 1 | 287 | 1 | 296 | 4.8e-134 | 486.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene85699.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85699.t1 | MTR_0558s0020 | 86.799 | 303 | 17 | 3 | 1 | 287 | 1 | 296 | 0.0 | 531 |
| MS.gene85699.t1 | MTR_1g083640 | 85.315 | 286 | 18 | 3 | 1 | 262 | 1 | 286 | 2.04e-176 | 488 |
| MS.gene85699.t1 | MTR_8g446020 | 73.927 | 303 | 16 | 4 | 1 | 287 | 1 | 256 | 8.36e-152 | 425 |
| MS.gene85699.t1 | MTR_2g014100 | 85.306 | 245 | 19 | 3 | 1 | 228 | 1 | 245 | 1.95e-145 | 409 |
| MS.gene85699.t1 | MTR_6g090505 | 86.522 | 230 | 15 | 2 | 73 | 286 | 1 | 230 | 4.89e-142 | 404 |
| MS.gene85699.t1 | MTR_0337s0040 | 69.403 | 268 | 20 | 4 | 1 | 252 | 1 | 222 | 4.24e-120 | 343 |
| MS.gene85699.t1 | MTR_3g008240 | 80.476 | 210 | 18 | 3 | 1 | 194 | 1 | 203 | 1.28e-114 | 328 |
| MS.gene85699.t1 | MTR_4g040430 | 79.524 | 210 | 15 | 4 | 1 | 194 | 1 | 198 | 3.21e-111 | 320 |
| MS.gene85699.t1 | MTR_5g071590 | 73.224 | 183 | 22 | 4 | 1 | 159 | 1 | 180 | 3.71e-88 | 261 |
| MS.gene85699.t1 | MTR_0019s0290 | 78.947 | 152 | 16 | 2 | 90 | 225 | 9 | 160 | 1.07e-78 | 236 |
| MS.gene85699.t1 | MTR_1g055240 | 82.517 | 143 | 9 | 2 | 98 | 224 | 2 | 144 | 3.38e-78 | 234 |
| MS.gene85699.t1 | MTR_1281s0010 | 85.294 | 102 | 7 | 1 | 27 | 120 | 1 | 102 | 8.46e-56 | 176 |
| MS.gene85699.t1 | MTR_8g046060 | 87.500 | 88 | 10 | 1 | 1 | 87 | 91 | 178 | 2.07e-41 | 142 |
| MS.gene85699.t1 | MTR_4g008820 | 82.759 | 87 | 8 | 1 | 1 | 87 | 125 | 204 | 1.69e-38 | 143 |
| MS.gene85699.t1 | MTR_6g051425 | 30.417 | 240 | 154 | 4 | 48 | 280 | 22 | 255 | 9.38e-25 | 102 |
| MS.gene85699.t1 | MTR_1g070355 | 27.984 | 243 | 156 | 7 | 49 | 283 | 80 | 311 | 1.69e-16 | 79.7 |
| MS.gene85699.t1 | MTR_0337s0050 | 83.333 | 36 | 6 | 0 | 251 | 286 | 14 | 49 | 1.02e-14 | 68.2 |
| MS.gene85699.t1 | MTR_1g013060 | 28.241 | 216 | 120 | 9 | 48 | 245 | 29 | 227 | 4.66e-12 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85699.t1 | AT4G38180 | 27.059 | 255 | 173 | 5 | 42 | 290 | 72 | 319 | 6.81e-21 | 93.2 |
| MS.gene85699.t1 | AT5G28530 | 25.101 | 247 | 165 | 5 | 48 | 279 | 60 | 301 | 1.75e-14 | 73.9 |
| MS.gene85699.t1 | AT5G28530 | 25.101 | 247 | 165 | 5 | 48 | 279 | 60 | 301 | 1.75e-14 | 73.9 |
| MS.gene85699.t1 | AT5G28530 | 25.101 | 247 | 165 | 5 | 48 | 279 | 60 | 301 | 1.77e-14 | 73.9 |
| MS.gene85699.t1 | AT1G10240 | 23.529 | 238 | 170 | 5 | 57 | 287 | 62 | 294 | 1.22e-11 | 65.5 |
| MS.gene85699.t1 | AT1G10240 | 23.529 | 238 | 170 | 5 | 57 | 287 | 62 | 294 | 1.22e-11 | 65.5 |
Find 37 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACTAGATTATTCTCTATT+TGG | 0.147857 | 6.1:-17414299 | MS.gene85699:CDS |
| ATAACACTGGATTCTCTATT+AGG | 0.309117 | 6.1:-17414889 | MS.gene85699:CDS |
| ACAAACTCAATAAAGTTGTT+AGG | 0.316138 | 6.1:+17414758 | None:intergenic |
| GGCGATATTGATTGTTTGTT+CGG | 0.357702 | 6.1:+17414249 | None:intergenic |
| GAGAACGTCTTATGAAGTTA+TGG | 0.394356 | 6.1:-17414540 | MS.gene85699:CDS |
| GTACAAAATACTAACCTAAT+GGG | 0.422907 | 6.1:+17414228 | None:intergenic |
| ATGTCTATAAGATATATAGA+TGG | 0.423693 | 6.1:-17414685 | MS.gene85699:CDS |
| GAAGTTATGGGTCAAGGAGT+TGG | 0.428685 | 6.1:-17414527 | MS.gene85699:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 6.1:-17414602 | MS.gene85699:CDS |
| AACAATCAATATCGCCCATT+AGG | 0.430510 | 6.1:-17414242 | MS.gene85699:intron |
| TCCATGATTCTTTATTGCTA+GGG | 0.444266 | 6.1:+17414969 | None:intergenic |
| TTCCATGATTCTTTATTGCT+AGG | 0.445442 | 6.1:+17414968 | None:intergenic |
| TCTAATGACAATTCGTCAAA+AGG | 0.456711 | 6.1:-17414459 | MS.gene85699:CDS |
| GCTTTAATAAATTCTAAAGC+TGG | 0.465202 | 6.1:-17414572 | MS.gene85699:CDS |
| AGAACGTCTTATGAAGTTAT+GGG | 0.466325 | 6.1:-17414539 | MS.gene85699:CDS |
| CGTTATGTTTGTAACAAACA+AGG | 0.494754 | 6.1:-17414806 | MS.gene85699:CDS |
| CCCCTAGCAATAAAGAATCA+TGG | 0.497001 | 6.1:-17414970 | MS.gene85699:CDS |
| TGTCTTGTGCAGCATCAACT+TGG | 0.502977 | 6.1:+17414354 | None:intergenic |
| TAACAAACAAGGTTTCAAGA+AGG | 0.511682 | 6.1:-17414795 | MS.gene85699:CDS |
| GGTAATTATGCATATAACAC+TGG | 0.523547 | 6.1:-17414902 | MS.gene85699:CDS |
| TGTCTATAAGATATATAGAT+GGG | 0.523615 | 6.1:-17414684 | MS.gene85699:CDS |
| TTTAAGTCCGAAAACCGAAA+AGG | 0.524077 | 6.1:+17414488 | None:intergenic |
| AAATGCCCTGGTTTATGTAA+TGG | 0.529222 | 6.1:+17414644 | None:intergenic |
| ACAAAATAAGAAATCAGATA+AGG | 0.530342 | 6.1:-17414840 | MS.gene85699:CDS |
| TAGACACTAAGCAGTTCAAA+AGG | 0.536783 | 6.1:+17414128 | None:intergenic |
| CCATGATTCTTTATTGCTAG+GGG | 0.537011 | 6.1:+17414970 | None:intergenic |
| AACAAACAAGGTTTCAAGAA+GGG | 0.554561 | 6.1:-17414794 | MS.gene85699:CDS |
| CGTCAAAAGGAGATGGTGGT+TGG | 0.556443 | 6.1:-17414446 | MS.gene85699:CDS |
| CTCAAGTAACACAAATGCCC+TGG | 0.558368 | 6.1:+17414632 | None:intergenic |
| GACAATTCGTCAAAAGGAGA+TGG | 0.566160 | 6.1:-17414453 | MS.gene85699:CDS |
| TAAGATATATAGATGGGATG+TGG | 0.567659 | 6.1:-17414678 | MS.gene85699:CDS |
| GTTATGGGTCAAGGAGTTGG+AGG | 0.578191 | 6.1:-17414524 | MS.gene85699:CDS |
| TCTTATGAAGTTATGGGTCA+AGG | 0.580120 | 6.1:-17414533 | MS.gene85699:CDS |
| TGTCATCCATTACATAAACC+AGG | 0.618164 | 6.1:-17414650 | MS.gene85699:CDS |
| AATTCGTCAAAAGGAGATGG+TGG | 0.636402 | 6.1:-17414450 | MS.gene85699:CDS |
| GGGTCAAGGAGTTGGAGGCA+CGG | 0.637387 | 6.1:-17414519 | MS.gene85699:CDS |
| GTCATCCATTACATAAACCA+GGG | 0.682651 | 6.1:-17414649 | MS.gene85699:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATAAGAAATCAGATA+AGG | - | chr6.1:17414382-17414401 | MS.gene85699:CDS | 20.0% |
| !! | ATGTCTATAAGATATATAGA+TGG | - | chr6.1:17414537-17414556 | MS.gene85699:CDS | 20.0% |
| !! | TGTCTATAAGATATATAGAT+GGG | - | chr6.1:17414538-17414557 | MS.gene85699:CDS | 20.0% |
| !!! | AAAGAGATTGAAGATTTTTA+TGG | - | chr6.1:17414299-17414318 | MS.gene85699:CDS | 20.0% |
| ! | ACAAACTCAATAAAGTTGTT+AGG | + | chr6.1:17414467-17414486 | None:intergenic | 25.0% |
| ! | AGTACAAAATACTAACCTAA+TGG | + | chr6.1:17414998-17415017 | None:intergenic | 25.0% |
| ! | CAACTAGATTATTCTCTATT+TGG | - | chr6.1:17414923-17414942 | MS.gene85699:CDS | 25.0% |
| ! | GTACAAAATACTAACCTAAT+GGG | + | chr6.1:17414997-17415016 | None:intergenic | 25.0% |
| ! | TGGATATCATAGTAAAAAGA+TGG | + | chr6.1:17414851-17414870 | None:intergenic | 25.0% |
| !! | CATTATGTTGTATTTTGTTC+TGG | - | chr6.1:17415048-17415067 | MS.gene85699:CDS | 25.0% |
| !!! | ACTTGGAATAATTTTGAAGT+TGG | - | chr6.1:17414502-17414521 | MS.gene85699:CDS | 25.0% |
| !!! | GCTTTAATAAATTCTAAAGC+TGG | - | chr6.1:17414650-17414669 | MS.gene85699:CDS | 25.0% |
| AACAAACAAGGTTTCAAGAA+GGG | - | chr6.1:17414428-17414447 | MS.gene85699:CDS | 30.0% | |
| AGAACGTCTTATGAAGTTAT+GGG | - | chr6.1:17414683-17414702 | MS.gene85699:CDS | 30.0% | |
| ATAACACTGGATTCTCTATT+AGG | - | chr6.1:17414333-17414352 | MS.gene85699:CDS | 30.0% | |
| CGTTATGTTTGTAACAAACA+AGG | - | chr6.1:17414416-17414435 | MS.gene85699:CDS | 30.0% | |
| GGTAATTATGCATATAACAC+TGG | - | chr6.1:17414320-17414339 | MS.gene85699:CDS | 30.0% | |
| GTGAAAGTGAAAGAAAAACT+TGG | - | chr6.1:17414485-17414504 | MS.gene85699:CDS | 30.0% | |
| TAACAAACAAGGTTTCAAGA+AGG | - | chr6.1:17414427-17414446 | MS.gene85699:CDS | 30.0% | |
| TAAGATATATAGATGGGATG+TGG | - | chr6.1:17414544-17414563 | MS.gene85699:CDS | 30.0% | |
| TCCATGATTCTTTATTGCTA+GGG | + | chr6.1:17414256-17414275 | None:intergenic | 30.0% | |
| TCTAATGACAATTCGTCAAA+AGG | - | chr6.1:17414763-17414782 | MS.gene85699:CDS | 30.0% | |
| TTCCATGATTCTTTATTGCT+AGG | + | chr6.1:17414257-17414276 | None:intergenic | 30.0% | |
| ! | TAGTATTTTGTACTTCTCTC+AGG | - | chr6.1:17415004-17415023 | MS.gene85699:CDS | 30.0% |
| !!! | AAGACATAGCTAGCATTTTT+TGG | - | chr6.1:17414885-17414904 | MS.gene85699:CDS | 30.0% |
| !!! | AGACATAGCTAGCATTTTTT+GGG | - | chr6.1:17414886-17414905 | MS.gene85699:CDS | 30.0% |
| AAATGCCCTGGTTTATGTAA+TGG | + | chr6.1:17414581-17414600 | None:intergenic | 35.0% | |
| AACAATCAATATCGCCCATT+AGG | - | chr6.1:17414980-17414999 | MS.gene85699:CDS | 35.0% | |
| CCATGATTCTTTATTGCTAG+GGG | + | chr6.1:17414255-17414274 | None:intergenic | 35.0% | |
| CGAGAAGTAAATGAAGTTCA+AGG | - | chr6.1:17414620-17414639 | MS.gene85699:CDS | 35.0% | |
| GAGAACGTCTTATGAAGTTA+TGG | - | chr6.1:17414682-17414701 | MS.gene85699:CDS | 35.0% | |
| GTCATCCATTACATAAACCA+GGG | - | chr6.1:17414573-17414592 | MS.gene85699:CDS | 35.0% | |
| TCTTATGAAGTTATGGGTCA+AGG | - | chr6.1:17414689-17414708 | MS.gene85699:CDS | 35.0% | |
| TGTCATCCATTACATAAACC+AGG | - | chr6.1:17414572-17414591 | MS.gene85699:CDS | 35.0% | |
| TTTAAGTCCGAAAACCGAAA+AGG | + | chr6.1:17414737-17414756 | None:intergenic | 35.0% | |
| ! | GAATCTTCCTTTTCGGTTTT+CGG | - | chr6.1:17414727-17414746 | MS.gene85699:CDS | 35.0% |
| ! | GGCGATATTGATTGTTTGTT+CGG | + | chr6.1:17414976-17414995 | None:intergenic | 35.0% |
| ! | TGTTGTATTTTGTTCTGGCA+TGG | - | chr6.1:17415053-17415072 | MS.gene85699:CDS | 35.0% |
| AATTCGTCAAAAGGAGATGG+TGG | - | chr6.1:17414772-17414791 | MS.gene85699:CDS | 40.0% | |
| CCCCTAGCAATAAAGAATCA+TGG | - | chr6.1:17414252-17414271 | MS.gene85699:CDS | 40.0% | |
| GACAATTCGTCAAAAGGAGA+TGG | - | chr6.1:17414769-17414788 | MS.gene85699:CDS | 40.0% | |
| TGTTCTGGCATGGATAGTAT+AGG | - | chr6.1:17415063-17415082 | MS.gene85699:CDS | 40.0% | |
| !!! | GCTAGCATTTTTTGGGCATA+TGG | - | chr6.1:17414893-17414912 | MS.gene85699:CDS | 40.0% |
| CTCAAGTAACACAAATGCCC+TGG | + | chr6.1:17414593-17414612 | None:intergenic | 45.0% | |
| CTGGCATGGATAGTATAGGT+AGG | - | chr6.1:17415067-17415086 | MS.gene85699:CDS | 45.0% | |
| GAAGTTATGGGTCAAGGAGT+TGG | - | chr6.1:17414695-17414714 | MS.gene85699:CDS | 45.0% | |
| TGTCTTGTGCAGCATCAACT+TGG | + | chr6.1:17414871-17414890 | None:intergenic | 45.0% | |
| ! | GCACGGAGAATCTTCCTTTT+CGG | - | chr6.1:17414720-17414739 | MS.gene85699:CDS | 45.0% |
| GTTATGGGTCAAGGAGTTGG+AGG | - | chr6.1:17414698-17414717 | MS.gene85699:CDS | 50.0% | |
| ! | CGTCAAAAGGAGATGGTGGT+TGG | - | chr6.1:17414776-17414795 | MS.gene85699:CDS | 50.0% |
| GGGTCAAGGAGTTGGAGGCA+CGG | - | chr6.1:17414703-17414722 | MS.gene85699:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 17414143 | 17415101 | 17414143 | ID=MS.gene85699 |
| chr6.1 | mRNA | 17414143 | 17415101 | 17414143 | ID=MS.gene85699.t1;Parent=MS.gene85699 |
| chr6.1 | exon | 17414243 | 17415101 | 17414243 | ID=MS.gene85699.t1.exon1;Parent=MS.gene85699.t1 |
| chr6.1 | CDS | 17414243 | 17415101 | 17414243 | ID=cds.MS.gene85699.t1;Parent=MS.gene85699.t1 |
| chr6.1 | exon | 17414143 | 17414159 | 17414143 | ID=MS.gene85699.t1.exon2;Parent=MS.gene85699.t1 |
| chr6.1 | CDS | 17414143 | 17414159 | 17414143 | ID=cds.MS.gene85699.t1;Parent=MS.gene85699.t1 |
| Gene Sequence |
| Protein sequence |
