AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85725

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85725.t1 MTR_6g035370 88.024 668 52 8 1 662 1 646 0.0 815
MS.gene85725.t1 MTR_1g098900 55.102 686 225 18 1 658 1 631 0.0 597
MS.gene85725.t1 MTR_1g098870 42.123 603 236 13 2 600 3 496 1.42e-125 384
MS.gene85725.t1 MTR_2g026725 31.597 288 145 7 281 568 115 350 1.35e-34 135
MS.gene85725.t1 MTR_2g026725 39.796 98 56 2 54 149 261 357 1.80e-16 82.0
MS.gene85725.t1 MTR_1g492790 52.756 127 55 2 54 175 113 239 1.45e-34 138
MS.gene85725.t1 MTR_1g492790 45.652 92 48 2 476 566 110 200 4.18e-19 91.3
MS.gene85725.t1 MTR_1g060990 64.583 96 31 2 46 140 124 217 5.71e-32 130
MS.gene85725.t1 MTR_1g060990 46.237 93 48 2 476 567 127 218 4.12e-19 91.7
MS.gene85725.t1 MTR_1g060990 37.383 107 67 0 251 357 264 370 4.61e-14 75.5
MS.gene85725.t1 MTR_7g114860 57.000 100 34 2 480 570 381 480 2.31e-28 119
MS.gene85725.t1 MTR_7g114860 41.837 98 47 2 55 142 381 478 7.70e-16 81.3
MS.gene85725.t1 MTR_1g081180 59.783 92 35 1 480 569 418 509 1.54e-26 114
MS.gene85725.t1 MTR_1g081180 39.785 93 53 2 53 142 416 508 5.94e-14 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85725.t1 AT1G76880 57.099 324 93 8 43 359 49 333 1.43e-93 301
MS.gene85725.t1 AT1G76880 65.584 154 38 3 458 598 382 533 4.05e-60 212
MS.gene85725.t1 AT1G76880 46.739 92 48 1 50 140 403 494 3.77e-19 92.0
MS.gene85725.t1 AT1G76880 47.253 91 47 1 479 569 60 149 6.27e-19 91.3
MS.gene85725.t1 AT1G76890 49.375 320 129 7 52 357 38 338 7.53e-83 273
MS.gene85725.t1 AT1G76890 64.029 139 40 3 459 597 378 506 4.02e-49 181
MS.gene85725.t1 AT1G76890 44.565 92 50 1 52 142 394 485 1.81e-19 93.2
MS.gene85725.t1 AT1G76890 43.182 88 49 1 479 566 40 126 5.01e-17 85.5
MS.gene85725.t1 AT5G28300 36.789 299 139 9 281 569 300 558 2.60e-44 168
MS.gene85725.t1 AT5G28300 41.667 108 52 4 48 144 452 559 2.51e-15 80.1
MS.gene85725.t1 AT5G03680 34.202 307 142 7 55 356 119 370 7.42e-44 166
MS.gene85725.t1 AT5G03680 44.211 95 51 2 480 573 119 212 5.05e-19 91.7
MS.gene85725.t1 AT1G33240 59.649 114 35 1 54 167 61 163 3.38e-38 150
MS.gene85725.t1 AT1G33240 59.649 114 35 1 54 167 61 163 6.67e-38 150
MS.gene85725.t1 AT3G10000 57.143 105 43 2 38 140 71 175 1.42e-30 126
MS.gene85725.t1 AT3G10000 44.086 93 51 1 476 567 84 176 1.50e-20 95.9
MS.gene85725.t1 AT3G10000 53.846 104 38 2 38 140 71 165 7.45e-27 115
MS.gene85725.t1 AT3G10000 41.304 92 45 1 476 567 84 166 2.08e-17 85.9
MS.gene85725.t1 AT5G47660 51.685 89 41 1 480 568 303 389 8.03e-22 98.6
MS.gene85725.t1 AT5G47660 47.059 85 40 3 55 137 303 384 4.57e-14 75.1
MS.gene85725.t1 AT1G33240 38.655 119 71 2 55 171 396 514 3.18e-19 92.4
MS.gene85725.t1 AT1G33240 54.430 79 25 1 89 167 57 124 1.91e-18 90.1

Find 196 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAAGATGCAAGTGTTAA+AGG 0.186987 6.1:+16665394 MS.gene85725:CDS
TTCAGGTTTGATGAAGAAAA+TGG 0.194543 6.1:+16667769 MS.gene85725:CDS
CCATTATGGGAGGAGATTTC+AGG 0.224552 6.1:+16667752 MS.gene85725:CDS
TTTACCACCTCTTCCTTCTT+TGG 0.238671 6.1:-16666583 None:intergenic
ATGGACCTAAAGGGCCATTA+TGG 0.251711 6.1:+16667738 MS.gene85725:CDS
TCGGTGTCGGTGTTGGTGTT+GGG 0.265380 6.1:-16667388 None:intergenic
GATATGAAGTACCAAGAAAA+TGG 0.297874 6.1:+16667719 MS.gene85725:CDS
GGACATGTCTTTGAATCTTC+TGG 0.297895 6.1:-16667881 None:intergenic
TCCTCCCATAATGGCCCTTT+AGG 0.309914 6.1:-16667743 None:intergenic
GTTGTTGTTGATGATGGTAT+TGG 0.315589 6.1:-16666870 None:intergenic
AACAGGCGCCACCAGCAGCC+AGG 0.316128 6.1:+16668035 MS.gene85725:CDS
AAGTTCGTCTTCGGCTTCTT+CGG 0.334124 6.1:+16666992 MS.gene85725:CDS
GGAGTTGCACAAGAGGTTCT+TGG 0.339414 6.1:+16667118 MS.gene85725:CDS
GTCGGTGTCGGTGTTGGTGT+TGG 0.352301 6.1:-16667389 None:intergenic
GTCACATCCGCCCTGGCTGC+TGG 0.353901 6.1:-16668046 None:intergenic
TACTTTGGCTAATAGGAAAA+TGG 0.355696 6.1:+16666485 MS.gene85725:intron
CTCGAATTCAAGTTCGTCTT+CGG 0.360610 6.1:+16666983 MS.gene85725:CDS
GTTGAAGATAAGGTCTGATA+TGG 0.365393 6.1:+16665363 MS.gene85725:CDS
AGAGGGTTATTAGTATTTGT+AGG 0.377717 6.1:-16666900 None:intergenic
TACTAAAGATGCTGCTGTTA+TGG 0.378392 6.1:+16667259 MS.gene85725:CDS
AAAGCAACTGTTTCTTGTCT+TGG 0.384139 6.1:-16665340 None:intergenic
TCAGGTTTGATGAAGAAAAT+GGG 0.384923 6.1:+16667770 MS.gene85725:CDS
AATAGGAAAATGGCAGATCT+TGG 0.385060 6.1:+16666495 MS.gene85725:intron
CAAGTGTTAAAGGTCCCTTA+TGG 0.386222 6.1:+16665404 MS.gene85725:CDS
TTGAGGTGGTGGTGGTTTGT+TGG 0.387596 6.1:-16668194 None:intergenic
ACTGGCAATGGTGCTGCTGC+TGG 0.390612 6.1:-16667413 None:intergenic
GCAATGGCCTCCACAACAAC+AGG 0.393419 6.1:+16668018 MS.gene85725:CDS
TGAAGAAAATGGGGTACAAT+AGG 0.394111 6.1:+16667780 MS.gene85725:CDS
ACAGCAGCAAGTGACGAATA+TGG 0.394354 6.1:+16667592 MS.gene85725:CDS
TGCAAAGGATTAATAGAGAA+AGG 0.396339 6.1:+16667204 MS.gene85725:CDS
CAAAGAGGTGCAAAGAGAAA+TGG 0.396790 6.1:+16667807 MS.gene85725:CDS
CCTGAAATCTCCTCCCATAA+TGG 0.399545 6.1:-16667752 None:intergenic
AGGGGTGGGAATAGTACTAA+TGG 0.401850 6.1:-16666721 None:intergenic
AACGATGATGGCACCGTTGA+TGG 0.403172 6.1:+16667982 MS.gene85725:CDS
GTCGGTGTTGGTGTTGGGGT+TGG 0.406448 6.1:-16667383 None:intergenic
CAGTTGCTTTGTTGAAGATA+AGG 0.412788 6.1:+16665353 MS.gene85725:CDS
GACTCAACAGTGTTGGGAAC+AGG 0.413588 6.1:+16665181 MS.gene85725:CDS
CCTTGAAACTTCATCCCATA+AGG 0.419165 6.1:-16665419 None:intergenic
GGAGTTTGTGTTTGAGTTTG+TGG 0.419992 6.1:-16666699 None:intergenic
GGAACAGGAGGAGGCAGCTC+AGG 0.420665 6.1:+16665196 MS.gene85725:CDS
GAGGGTGTGGATAGAAGTTT+TGG 0.422223 6.1:+16665304 MS.gene85725:CDS
TGTGTTTGAGTTTGTGGTGG+TGG 0.423701 6.1:-16666693 None:intergenic
AGAAATGAAGCTGTTGTTGT+AGG 0.430108 6.1:-16666741 None:intergenic
GTAATAGTAATTTGAGGAAG+AGG 0.435660 6.1:-16666918 None:intergenic
TGTGTGTAAGACATTGGAAT+TGG 0.436193 6.1:-16666825 None:intergenic
ACCAACTTGATGCTCTATAC+AGG 0.438141 6.1:+16667912 MS.gene85725:CDS
TGGACCTAAAGGGCCATTAT+GGG 0.446325 6.1:+16667739 MS.gene85725:CDS
GGTGGTGGTGGTGTTATTGA+AGG 0.449272 6.1:-16666678 None:intergenic
GGTATTGCTGGTATTGGTAT+TGG 0.450653 6.1:-16667452 None:intergenic
ACGGTGTTGTTGTTCAATAC+AGG 0.451355 6.1:-16667323 None:intergenic
AAGTGTTAAAGGTCCCTTAT+GGG 0.453094 6.1:+16665405 MS.gene85725:CDS
GACGGAAGAGGAAGAGGAAA+TGG 0.453743 6.1:+16667048 MS.gene85725:CDS
CGAATCGACGGAAGAGGAAG+AGG 0.460674 6.1:+16667042 MS.gene85725:CDS
GGTGTAGGTATTGCTGGTAT+TGG 0.461156 6.1:-16667458 None:intergenic
AGCTGCTGCGTCGAGGCCTG+AGG 0.462044 6.1:+16667958 MS.gene85725:CDS
GAAATGAAGCTGTTGTTGTA+GGG 0.465028 6.1:-16666740 None:intergenic
AATGGTGCTGCTGCTGGTGT+CGG 0.467443 6.1:-16667407 None:intergenic
GCTGCTGCTGGTGTCGGTGT+CGG 0.467777 6.1:-16667401 None:intergenic
CCTTATGGGATGAAGTTTCA+AGG 0.471695 6.1:+16665419 MS.gene85725:CDS
GCATCAAGTTGGTGAAAGTA+AGG 0.472098 6.1:-16667902 None:intergenic
CTAGGGGACTCAACAGTGTT+GGG 0.473609 6.1:+16665175 MS.gene85725:CDS
TGTGGAGGCCATTGCTGCTC+CGG 0.474042 6.1:-16668010 None:intergenic
GGCAATGGTACTGCTGGTAC+TGG 0.477694 6.1:-16667431 None:intergenic
GTTTGAGTTTGTGGTGGTGG+TGG 0.478900 6.1:-16666690 None:intergenic
TGGAAGTAGTAGAAATGAAG+AGG 0.480126 6.1:+16665285 MS.gene85725:CDS
GCAAAGGATTAATAGAGAAA+GGG 0.481748 6.1:+16667205 MS.gene85725:CDS
TACCAAGAAAATGGACCTAA+AGG 0.481995 6.1:+16667728 MS.gene85725:CDS
GCTGGTGGCGCCTGTTGTTG+TGG 0.482373 6.1:-16668028 None:intergenic
TTTCTTTGAGAGACTAATGA+AGG 0.482390 6.1:+16667076 MS.gene85725:CDS
CTTGAAACTTCATCCCATAA+GGG 0.483873 6.1:-16665418 None:intergenic
TAGTAGAAATGAAGAGGGTG+TGG 0.484511 6.1:+16665291 MS.gene85725:CDS
GAGGGTTATTAGTATTTGTA+GGG 0.485687 6.1:-16666899 None:intergenic
CGGTGTCGGTGTTGGTGTTG+GGG 0.486815 6.1:-16667387 None:intergenic
GGAAACGACAACAATGGAAG+GGG 0.487557 6.1:+16667016 MS.gene85725:CDS
CTCCATGGTCACATCCGCCC+TGG 0.491677 6.1:-16668053 None:intergenic
ATAGGAAAATGGCAGATCTT+GGG 0.492836 6.1:+16666496 MS.gene85725:intron
ACCAAGAAAATGGACCTAAA+GGG 0.493139 6.1:+16667729 MS.gene85725:CDS
ACATCAGCAGCAGCAACAAC+AGG 0.496241 6.1:+16667559 MS.gene85725:CDS
GCAGCAGCAACAACAGGTTC+AGG 0.497898 6.1:+16667565 MS.gene85725:CDS
GCTGGTGTCGGTGTCGGTGT+TGG 0.501369 6.1:-16667395 None:intergenic
GGAGCTTACCATGATGGTGG+TGG 0.501555 6.1:+16665229 MS.gene85725:CDS
GGCAGCTCAGGAGATGGAGC+AGG 0.501746 6.1:+16665208 MS.gene85725:CDS
GGGGATCGAATCGACGGAAG+AGG 0.501747 6.1:+16667036 MS.gene85725:CDS
GCTTACCATGATGGTGGTGG+GGG 0.502473 6.1:+16665232 MS.gene85725:CDS
GGAATTGGAAGTGTAGTTGA+TGG 0.503022 6.1:-16666810 None:intergenic
TGTTGCGGTGTAGGTATTGC+TGG 0.503047 6.1:-16667464 None:intergenic
GGCACCGTTGATGGTTCAGC+CGG 0.506059 6.1:+16667991 MS.gene85725:CDS
TGTTGCTGCTGCTGATGTTG+TGG 0.506243 6.1:-16667554 None:intergenic
GAAGGTGATTGATGCATTGA+AGG 0.506401 6.1:-16666660 None:intergenic
AAAGAGGTGCAAAGAGAAAT+GGG 0.507157 6.1:+16667808 MS.gene85725:CDS
TTCGTCTTCGGCTTCTTCGG+AGG 0.514052 6.1:+16666995 MS.gene85725:CDS
GTTTGAACTTGTTGCGGTGT+AGG 0.516119 6.1:-16667473 None:intergenic
CACTCCTATGCAGCTATGCT+AGG 0.516756 6.1:+16665157 None:intergenic
AACAGCACCGGTCATTCCTC+AGG 0.519741 6.1:+16667520 MS.gene85725:CDS
GGTGCCATCATCGTTCCCTC+AGG 0.524674 6.1:-16667974 None:intergenic
AGCTTACCATGATGGTGGTG+GGG 0.524813 6.1:+16665231 MS.gene85725:CDS
ACTCCTATGCAGCTATGCTA+GGG 0.525198 6.1:+16665158 None:intergenic
GAGGTGCTAGAGAAGAAGCT+TGG 0.525337 6.1:+16667168 MS.gene85725:CDS
TGGTGGAAAGTGTGTAGGGT+TGG 0.526086 6.1:-16666850 None:intergenic
GAGCTTACCATGATGGTGGT+GGG 0.527151 6.1:+16665230 MS.gene85725:CDS
GAGAAAAGAGAAAGAGAGAG+AGG 0.527477 6.1:+16667149 MS.gene85725:CDS
TGGTTCAGCCGGAGCAGCAA+TGG 0.528420 6.1:+16668002 MS.gene85725:CDS
GCTAGGGGACTCAACAGTGT+TGG 0.529101 6.1:+16665174 MS.gene85725:CDS
ACAGTGTTGGGAACAGGAGG+AGG 0.532710 6.1:+16665187 MS.gene85725:CDS
GGGGTGGGAATAGTACTAAT+GGG 0.535590 6.1:-16666720 None:intergenic
TGATGCATGCAAGTTCATCT+AGG 0.535689 6.1:+16667648 MS.gene85725:CDS
AACAAGAGGAGTTGCACAAG+AGG 0.535913 6.1:+16667111 MS.gene85725:CDS
GAAATATTAGCGAATGAAGA+TGG 0.536488 6.1:-16666948 None:intergenic
TGTTGTTGCACTGTTACAGT+TGG 0.536680 6.1:-16667497 None:intergenic
GCTGCTGCGTCGAGGCCTGA+GGG 0.536821 6.1:+16667959 MS.gene85725:CDS
ACATCCGCCCTGGCTGCTGG+TGG 0.538679 6.1:-16668043 None:intergenic
TGCTCCGGCTGAACCATCAA+CGG 0.540154 6.1:-16667995 None:intergenic
TAATAGTAATTTGAGGAAGA+GGG 0.542471 6.1:-16666917 None:intergenic
GGCGAAGCCTGAGGAATGAC+CGG 0.543584 6.1:-16667527 None:intergenic
GAGGCCTGAGGGAACGATGA+TGG 0.544953 6.1:+16667970 MS.gene85725:CDS
GGAGGAGGCAGCTCAGGAGA+TGG 0.546943 6.1:+16665202 MS.gene85725:CDS
GGAGGTGTAGAGTTTAACAA+TGG 0.547437 6.1:-16666774 None:intergenic
TGAAGGAAAGCAACAAGAAG+AGG 0.547925 6.1:+16667858 MS.gene85725:CDS
GGAAGAGGTGGTAAATCAGA+TGG 0.550653 6.1:+16666591 MS.gene85725:CDS
TTGGATTGTGTGTAAGACAT+TGG 0.551286 6.1:-16666831 None:intergenic
ACAGGCGCCACCAGCAGCCA+GGG 0.555235 6.1:+16668036 MS.gene85725:CDS
GTGCTAGAGAAGAAGCTTGG+AGG 0.555286 6.1:+16667171 MS.gene85725:CDS
TTGTTGTTGTTGAGGAATGA+CGG 0.557825 6.1:-16667342 None:intergenic
TATTAGCGAATGAAGATGGT+GGG 0.558390 6.1:-16666944 None:intergenic
GTTGTTGATGATGGTATTGG+TGG 0.559014 6.1:-16666867 None:intergenic
TGCTGCTGATGTTGTGGCAA+CGG 0.560332 6.1:-16667548 None:intergenic
GAGGAAACGACAACAATGGA+AGG 0.562272 6.1:+16667014 MS.gene85725:CDS
TGCGGCGCTTGTTGTTGTTG+AGG 0.562772 6.1:-16667350 None:intergenic
TCAACAACAACAAGCGCCGC+AGG 0.568754 6.1:+16667352 MS.gene85725:CDS
TGAAGCTGTTGTTGTAGGGG+TGG 0.568902 6.1:-16666736 None:intergenic
ATTAGCGAATGAAGATGGTG+GGG 0.569029 6.1:-16666943 None:intergenic
TATCACAAGAGAACCAAAGA+AGG 0.569305 6.1:+16666570 MS.gene85725:CDS
GGAGGTTGCAAGAGATGCAA+AGG 0.569352 6.1:+16667189 MS.gene85725:CDS
TGATCGGCCGCGTCGAGATG+AGG 0.570258 6.1:+16668096 MS.gene85725:CDS
TTTCAGGTGATGATGAAAGA+GGG 0.571339 6.1:+16665260 MS.gene85725:CDS
AAGAGAACCAAAGAAGGAAG+AGG 0.573797 6.1:+16666576 MS.gene85725:CDS
GGTATTGGTGGAAAGTGTGT+AGG 0.574192 6.1:-16666855 None:intergenic
TTCATTCGCTAATATTTCAG+CGG 0.578885 6.1:+16666953 MS.gene85725:CDS
CAGGCAGAAGAACAAGGTGG+AGG 0.580136 6.1:+16667931 MS.gene85725:CDS
CAGGTTTGATGAAGAAAATG+GGG 0.580467 6.1:+16667771 MS.gene85725:CDS
GCTGGTATTGGTATTGGCAA+TGG 0.581527 6.1:-16667446 None:intergenic
GGTATTGGCAATGGTACTGC+TGG 0.582926 6.1:-16667437 None:intergenic
GGTACTGCTGGTACTGGCAA+TGG 0.588980 6.1:-16667425 None:intergenic
GGAGCAGGAGCTTACCATGA+TGG 0.589395 6.1:+16665223 MS.gene85725:CDS
GTTTGTGTTTGAGTTTGTGG+TGG 0.590703 6.1:-16666696 None:intergenic
CTGAGAATGCTGAGAATGAT+CGG 0.591465 6.1:+16668080 MS.gene85725:CDS
ATTTCAGGTGATGATGAAAG+AGG 0.592121 6.1:+16665259 MS.gene85725:CDS
AGAACCAAAGAAGGAAGAGG+TGG 0.595193 6.1:+16666579 MS.gene85725:CDS
GATGACGAGGACGAAGATGA+AGG 0.597081 6.1:+16668148 MS.gene85725:CDS
AGCATTCTCAGCATCCTCCA+TGG 0.598685 6.1:-16668068 None:intergenic
GAATATTATGACGATGATGA+CGG 0.600596 6.1:+16668121 MS.gene85725:CDS
GGCGCCACCAGCAGCCAGGG+CGG 0.602641 6.1:+16668039 MS.gene85725:CDS
AGGGTGCAGCTGCTGCGTCG+AGG 0.603837 6.1:+16667951 MS.gene85725:CDS
GGAAGTAGTAGAAATGAAGA+GGG 0.606377 6.1:+16665286 MS.gene85725:CDS
TTCGGAGGAAACGACAACAA+TGG 0.607000 6.1:+16667010 MS.gene85725:CDS
GGAGGTTGTTGAGAAACAAG+AGG 0.607799 6.1:+16667097 MS.gene85725:CDS
CTTTGAGAGACTAATGAAGG+AGG 0.607887 6.1:+16667079 MS.gene85725:CDS
GCCTGTATAGAGCATCAAGT+TGG 0.608740 6.1:-16667913 None:intergenic
GTATTGGTGGAAAGTGTGTA+GGG 0.609236 6.1:-16666854 None:intergenic
GAAGAGGAAGAGGAAATGGA+AGG 0.609651 6.1:+16667052 MS.gene85725:CDS
TTAGCGAATGAAGATGGTGG+GGG 0.612373 6.1:-16666942 None:intergenic
CAAGTATTTCAAGAAAGTGA+AGG 0.613019 6.1:+16667841 MS.gene85725:CDS
GTTGATGGAACAGTGGTGGT+AGG 0.616670 6.1:-16666795 None:intergenic
AAGGTGATTGATGCATTGAA+GGG 0.619014 6.1:-16666659 None:intergenic
TCAACAGTGTTGGGAACAGG+AGG 0.620361 6.1:+16665184 MS.gene85725:CDS
GATGGAACAGTGGTGGTAGG+AGG 0.624878 6.1:-16666792 None:intergenic
GAAGCTGTTGTTGTAGGGGT+GGG 0.625788 6.1:-16666735 None:intergenic
TCTATACAGGCAGAAGAACA+AGG 0.628687 6.1:+16667925 MS.gene85725:CDS
ATATTAGCGAATGAAGATGG+TGG 0.629833 6.1:-16666945 None:intergenic
ACCTAAAGGGCCATTATGGG+AGG 0.631216 6.1:+16667742 MS.gene85725:CDS
TGTGGCAACGGCGAAGCCTG+AGG 0.633518 6.1:-16667536 None:intergenic
TTCAGGTGATGATGAAAGAG+GGG 0.634874 6.1:+16665261 MS.gene85725:CDS
AGCCAGGGCGGATGTGACCA+TGG 0.636181 6.1:+16668051 MS.gene85725:CDS
GGTGTAGAGTTTAACAATGG+AGG 0.640467 6.1:-16666771 None:intergenic
CTCCTATGCAGCTATGCTAG+GGG 0.640615 6.1:+16665159 None:intergenic
AGGAAACGACAACAATGGAA+GGG 0.641866 6.1:+16667015 MS.gene85725:CDS
AGGCAGAAGAACAAGGTGGA+GGG 0.643787 6.1:+16667932 MS.gene85725:CDS
GTTGGAGTTTGAACTTGTTG+CGG 0.644658 6.1:-16667479 None:intergenic
GTGTTGTTGTTCAATACAGG+AGG 0.651178 6.1:-16667320 None:intergenic
AGTGCAACAACAAACAGCAC+CGG 0.652497 6.1:+16667508 MS.gene85725:CDS
GGGGTTGGTGTAGTTTCCTG+CGG 0.653086 6.1:-16667368 None:intergenic
GGTGGCGCCTGTTGTTGTGG+AGG 0.655534 6.1:-16668025 None:intergenic
TCAGGTGATGATGAAAGAGG+GGG 0.658562 6.1:+16665262 MS.gene85725:CDS
AAATGAAGCTGTTGTTGTAG+GGG 0.668647 6.1:-16666739 None:intergenic
GAAACGACAACAATGGAAGG+GGG 0.686144 6.1:+16667017 MS.gene85725:CDS
TGCATGCAAGTTCATCTAGG+TGG 0.686396 6.1:+16667651 MS.gene85725:CDS
GCAGGAGCTTACCATGATGG+TGG 0.707615 6.1:+16665226 MS.gene85725:CDS
GTTGCGAAAAGTGACAACAA+TGG 0.712598 6.1:+16667617 MS.gene85725:CDS
AAGTGTAGTTGATGGAACAG+TGG 0.713309 6.1:-16666802 None:intergenic
AGGTAGTGACAATTATGAAG+TGG 0.722373 6.1:+16668168 MS.gene85725:CDS
GGTACAATAGGAATGCAAAG+AGG 0.724275 6.1:+16667792 MS.gene85725:CDS
TGTAGTTGATGGAACAGTGG+TGG 0.725823 6.1:-16666799 None:intergenic
CAGGGCGGATGTGACCATGG+AGG 0.727693 6.1:+16668054 MS.gene85725:CDS
ATACAGGCAGAAGAACAAGG+TGG 0.746845 6.1:+16667928 MS.gene85725:CDS
TATTCTTCCTCATCTCGACG+CGG 0.761362 6.1:-16668103 None:intergenic
TGATGACGGTGATGATGACG+AGG 0.768255 6.1:+16668135 MS.gene85725:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATTAAAAATATACATGA+AGG - chr6.1:16665842-16665861 None:intergenic 10.0%
!!! ATTAATTTTGTCTTTATTTT+TGG + chr6.1:16666461-16666480 MS.gene85725:intron 10.0%
!!! ATTTAATTTTTCAATTTTTG+AGG + chr6.1:16665633-16665652 MS.gene85725:intron 10.0%
!!! TATTTTTTTTATCTTTGATT+TGG + chr6.1:16665742-16665761 MS.gene85725:intron 10.0%
!!! TTAATTTTGTCTTTATTTTT+GGG + chr6.1:16666462-16666481 MS.gene85725:intron 10.0%
!! GTTGAAAAATAAAAATATGA+TGG + chr6.1:16666086-16666105 MS.gene85725:intron 15.0%
!!! TATTTTTCAACACTAAAAAT+GGG - chr6.1:16666077-16666096 None:intergenic 15.0%
!!! TTATTTTTCAACACTAAAAA+TGG - chr6.1:16666078-16666097 None:intergenic 15.0%
!! AACTTAAGTGATTAAGATAA+GGG - chr6.1:16666278-16666297 None:intergenic 20.0%
!! TAAATTAATAAATGGGATTG+AGG - chr6.1:16665618-16665637 None:intergenic 20.0%
!! TAACTTAAGTGATTAAGATA+AGG - chr6.1:16666279-16666298 None:intergenic 20.0%
!! TGTTATCACATTAAATCTTT+AGG - chr6.1:16666412-16666431 None:intergenic 20.0%
!!! AAACTTTTCTTTGCATTTTT+TGG - chr6.1:16666535-16666554 None:intergenic 20.0%
!!! ATTAAAAAGTTTGTTTCTCA+TGG - chr6.1:16666154-16666173 None:intergenic 20.0%
!!! TATATCAAAGTTGATGTAAT+TGG - chr6.1:16666233-16666252 None:intergenic 20.0%
!!! TTCAAGTTTGTAACTTTATT+TGG - chr6.1:16666007-16666026 None:intergenic 20.0%
!!! TTTTTTTTTCAGATTTCTGA+AGG - chr6.1:16665529-16665548 None:intergenic 20.0%
! AAAAATGGGTAACTGAAATA+AGG - chr6.1:16666063-16666082 None:intergenic 25.0%
! AAAATGGGTAACTGAAATAA+GGG - chr6.1:16666062-16666081 None:intergenic 25.0%
! TAATAGTAATTTGAGGAAGA+GGG - chr6.1:16666920-16666939 None:intergenic 25.0%
! TAGGATAAGAAGCAATTAAT+TGG - chr6.1:16666393-16666412 None:intergenic 25.0%
! TCAATAATTCAGATTTCTCA+AGG - chr6.1:16665683-16665702 None:intergenic 25.0%
AGACAACCAAAGAATTGAAA+TGG - chr6.1:16666308-16666327 None:intergenic 30.0%
AGAGGGTTATTAGTATTTGT+AGG - chr6.1:16666903-16666922 None:intergenic 30.0%
CAAAATCTTTGTTCACACTT+TGG + chr6.1:16666116-16666135 MS.gene85725:intron 30.0%
CAAGTATTTCAAGAAAGTGA+AGG + chr6.1:16667841-16667860 MS.gene85725:CDS 30.0%
GAAATATTAGCGAATGAAGA+TGG - chr6.1:16666951-16666970 None:intergenic 30.0%
GAATATTATGACGATGATGA+CGG + chr6.1:16668121-16668140 MS.gene85725:CDS 30.0%
GAGGGTTATTAGTATTTGTA+GGG - chr6.1:16666902-16666921 None:intergenic 30.0%
GCAAAGGATTAATAGAGAAA+GGG + chr6.1:16667205-16667224 MS.gene85725:CDS 30.0%
GTAATAGTAATTTGAGGAAG+AGG - chr6.1:16666921-16666940 None:intergenic 30.0%
TACTTTGGCTAATAGGAAAA+TGG + chr6.1:16666485-16666504 MS.gene85725:intron 30.0%
TCAGGTTTGATGAAGAAAAT+GGG + chr6.1:16667770-16667789 MS.gene85725:CDS 30.0%
TGCAAAGGATTAATAGAGAA+AGG + chr6.1:16667204-16667223 MS.gene85725:CDS 30.0%
TTCAAAGATGCAAGTGTTAA+AGG + chr6.1:16665394-16665413 MS.gene85725:CDS 30.0%
TTCATTCGCTAATATTTCAG+CGG + chr6.1:16666953-16666972 MS.gene85725:CDS 30.0%
! GATATGAAGTACCAAGAAAA+TGG + chr6.1:16667719-16667738 MS.gene85725:CDS 30.0%
! TTCAGGTTTGATGAAGAAAA+TGG + chr6.1:16667769-16667788 MS.gene85725:CDS 30.0%
! TTTCTTTGAGAGACTAATGA+AGG + chr6.1:16667076-16667095 MS.gene85725:CDS 30.0%
!! GGAAGAAAATCTTTGCTTTT+TGG - chr6.1:16666038-16666057 None:intergenic 30.0%
!! TTTGTTGTTGTTGTTGATGA+TGG - chr6.1:16666879-16666898 None:intergenic 30.0%
!!! GTCTTTATTTTTGGGTACTT+TGG + chr6.1:16666470-16666489 MS.gene85725:intron 30.0%
!!! TTTTTTCAGATTTCTGAAGG+TGG - chr6.1:16665526-16665545 None:intergenic 30.0%
AAATGAAGCTGTTGTTGTAG+GGG - chr6.1:16666742-16666761 None:intergenic 35.0%
AAGGTGATTGATGCATTGAA+GGG - chr6.1:16666662-16666681 None:intergenic 35.0%
AAGTGATTAAGATAAGGGAG+AGG - chr6.1:16666273-16666292 None:intergenic 35.0%
AAGTGTTAAAGGTCCCTTAT+GGG + chr6.1:16665405-16665424 MS.gene85725:CDS 35.0%
AATAGGAAAATGGCAGATCT+TGG + chr6.1:16666495-16666514 MS.gene85725:intron 35.0%
ACCAAGAAAATGGACCTAAA+GGG + chr6.1:16667729-16667748 MS.gene85725:CDS 35.0%
AGAAATGAAGCTGTTGTTGT+AGG - chr6.1:16666744-16666763 None:intergenic 35.0%
AGGTAGTGACAATTATGAAG+TGG + chr6.1:16668168-16668187 MS.gene85725:CDS 35.0%
ATAGGAAAATGGCAGATCTT+GGG + chr6.1:16666496-16666515 MS.gene85725:intron 35.0%
ATATTAGCGAATGAAGATGG+TGG - chr6.1:16666948-16666967 None:intergenic 35.0%
CAGGTTTGATGAAGAAAATG+GGG + chr6.1:16667771-16667790 MS.gene85725:CDS 35.0%
CTTGAAACTTCATCCCATAA+GGG - chr6.1:16665421-16665440 None:intergenic 35.0%
CTTGCTCCATTTCAATTCTT+TGG + chr6.1:16666299-16666318 MS.gene85725:intron 35.0%
GAAATGAAGCTGTTGTTGTA+GGG - chr6.1:16666743-16666762 None:intergenic 35.0%
GGAAGTAGTAGAAATGAAGA+GGG + chr6.1:16665286-16665305 MS.gene85725:CDS 35.0%
GTTGAAGATAAGGTCTGATA+TGG + chr6.1:16665363-16665382 MS.gene85725:CDS 35.0%
TACCAAGAAAATGGACCTAA+AGG + chr6.1:16667728-16667747 MS.gene85725:CDS 35.0%
TACTAAAGATGCTGCTGTTA+TGG + chr6.1:16667259-16667278 MS.gene85725:CDS 35.0%
TATCACAAGAGAACCAAAGA+AGG + chr6.1:16666570-16666589 MS.gene85725:CDS 35.0%
TATTAGCGAATGAAGATGGT+GGG - chr6.1:16666947-16666966 None:intergenic 35.0%
TGAAGAAAATGGGGTACAAT+AGG + chr6.1:16667780-16667799 MS.gene85725:CDS 35.0%
TGGAAGTAGTAGAAATGAAG+AGG + chr6.1:16665285-16665304 MS.gene85725:CDS 35.0%
TGTGTGTAAGACATTGGAAT+TGG - chr6.1:16666828-16666847 None:intergenic 35.0%
TTGGATTGTGTGTAAGACAT+TGG - chr6.1:16666834-16666853 None:intergenic 35.0%
! AAAGAGGTGCAAAGAGAAAT+GGG + chr6.1:16667808-16667827 MS.gene85725:CDS 35.0%
! AAAGCAACTGTTTCTTGTCT+TGG - chr6.1:16665343-16665362 None:intergenic 35.0%
! ATTTCAGGTGATGATGAAAG+AGG + chr6.1:16665259-16665278 MS.gene85725:CDS 35.0%
! CAGTTGCTTTGTTGAAGATA+AGG + chr6.1:16665353-16665372 MS.gene85725:CDS 35.0%
! TTGTTGTTGTTGAGGAATGA+CGG - chr6.1:16667345-16667364 None:intergenic 35.0%
! TTTCAGGTGATGATGAAAGA+GGG + chr6.1:16665260-16665279 MS.gene85725:CDS 35.0%
!! CATTGAAGGGTTGTTTTCAA+GGG - chr6.1:16666649-16666668 None:intergenic 35.0%
!! GAAGTTTTGGTGGTAATAGA+TGG + chr6.1:16665317-16665336 MS.gene85725:CDS 35.0%
!! GTTGTTGTTGATGATGGTAT+TGG - chr6.1:16666873-16666892 None:intergenic 35.0%
!! TTTTGGGTACTTTGGCTAAT+AGG + chr6.1:16666478-16666497 MS.gene85725:intron 35.0%
AAGAGAACCAAAGAAGGAAG+AGG + chr6.1:16666576-16666595 MS.gene85725:CDS 40.0%
ACCAACTTGATGCTCTATAC+AGG + chr6.1:16667912-16667931 MS.gene85725:CDS 40.0%
AGGAAACGACAACAATGGAA+GGG + chr6.1:16667015-16667034 MS.gene85725:CDS 40.0%
ATGGGTAACTGAAATAAGGG+AGG - chr6.1:16666059-16666078 None:intergenic 40.0%
ATTAGCGAATGAAGATGGTG+GGG - chr6.1:16666946-16666965 None:intergenic 40.0%
CAAGTGTTAAAGGTCCCTTA+TGG + chr6.1:16665404-16665423 MS.gene85725:CDS 40.0%
CCTTATGGGATGAAGTTTCA+AGG + chr6.1:16665419-16665438 MS.gene85725:CDS 40.0%
CCTTGAAACTTCATCCCATA+AGG - chr6.1:16665422-16665441 None:intergenic 40.0%
CTGAGAATGCTGAGAATGAT+CGG + chr6.1:16668080-16668099 MS.gene85725:CDS 40.0%
GAAGGTGATTGATGCATTGA+AGG - chr6.1:16666663-16666682 None:intergenic 40.0%
GAGAAAAGAGAAAGAGAGAG+AGG + chr6.1:16667149-16667168 MS.gene85725:CDS 40.0%
GGACATGTCTTTGAATCTTC+TGG - chr6.1:16667884-16667903 None:intergenic 40.0%
GGTACAATAGGAATGCAAAG+AGG + chr6.1:16667792-16667811 MS.gene85725:CDS 40.0%
GGTGTAGAGTTTAACAATGG+AGG - chr6.1:16666774-16666793 None:intergenic 40.0%
GTTGCGAAAAGTGACAACAA+TGG + chr6.1:16667617-16667636 MS.gene85725:CDS 40.0%
TAGTAGAAATGAAGAGGGTG+TGG + chr6.1:16665291-16665310 MS.gene85725:CDS 40.0%
TCTATACAGGCAGAAGAACA+AGG + chr6.1:16667925-16667944 MS.gene85725:CDS 40.0%
TGAAGGAAAGCAACAAGAAG+AGG + chr6.1:16667858-16667877 MS.gene85725:CDS 40.0%
TGATGCATGCAAGTTCATCT+AGG + chr6.1:16667648-16667667 MS.gene85725:CDS 40.0%
TGTTGTTGCACTGTTACAGT+TGG - chr6.1:16667500-16667519 None:intergenic 40.0%
TTTACCACCTCTTCCTTCTT+TGG - chr6.1:16666586-16666605 None:intergenic 40.0%
! AATGCCTCAACCTCAGTTTT+TGG - chr6.1:16667677-16667696 None:intergenic 40.0%
! CAAAGAGGTGCAAAGAGAAA+TGG + chr6.1:16667807-16667826 MS.gene85725:CDS 40.0%
! CTCGAATTCAAGTTCGTCTT+CGG + chr6.1:16666983-16667002 MS.gene85725:CDS 40.0%
! CTTTGAGAGACTAATGAAGG+AGG + chr6.1:16667079-16667098 MS.gene85725:CDS 40.0%
! GGAATTGGAAGTGTAGTTGA+TGG - chr6.1:16666813-16666832 None:intergenic 40.0%
! GGAGGTGTAGAGTTTAACAA+TGG - chr6.1:16666777-16666796 None:intergenic 40.0%
! GGAGTTTGTGTTTGAGTTTG+TGG - chr6.1:16666702-16666721 None:intergenic 40.0%
! GGGGGTGTAATAGTAATTTG+AGG - chr6.1:16666927-16666946 None:intergenic 40.0%
! GTATTGGTGGAAAGTGTGTA+GGG - chr6.1:16666857-16666876 None:intergenic 40.0%
! GTGTTGTTGTTCAATACAGG+AGG - chr6.1:16667323-16667342 None:intergenic 40.0%
! GTTTGTGTTTGAGTTTGTGG+TGG - chr6.1:16666699-16666718 None:intergenic 40.0%
! TTCAGGTGATGATGAAAGAG+GGG + chr6.1:16665261-16665280 MS.gene85725:CDS 40.0%
!! AAGTGTAGTTGATGGAACAG+TGG - chr6.1:16666805-16666824 None:intergenic 40.0%
!! ACGGTGTTGTTGTTCAATAC+AGG - chr6.1:16667326-16667345 None:intergenic 40.0%
!! GCATCAAGTTGGTGAAAGTA+AGG - chr6.1:16667905-16667924 None:intergenic 40.0%
!! GCATTGAAGGGTTGTTTTCA+AGG - chr6.1:16666650-16666669 None:intergenic 40.0%
!! GGTATTGCTGGTATTGGTAT+TGG - chr6.1:16667455-16667474 None:intergenic 40.0%
!! GTTGGAGTTTGAACTTGTTG+CGG - chr6.1:16667482-16667501 None:intergenic 40.0%
!! GTTGTTGATGATGGTATTGG+TGG - chr6.1:16666870-16666889 None:intergenic 40.0%
AACAAGAGGAGTTGCACAAG+AGG + chr6.1:16667111-16667130 MS.gene85725:CDS 45.0%
AAGTTCGTCTTCGGCTTCTT+CGG + chr6.1:16666992-16667011 MS.gene85725:CDS 45.0%
ACAGCAGCAAGTGACGAATA+TGG + chr6.1:16667592-16667611 MS.gene85725:CDS 45.0%
AGAACCAAAGAAGGAAGAGG+TGG + chr6.1:16666579-16666598 MS.gene85725:CDS 45.0%
AGGGGTGGGAATAGTACTAA+TGG - chr6.1:16666724-16666743 None:intergenic 45.0%
AGTGCAACAACAAACAGCAC+CGG + chr6.1:16667508-16667527 MS.gene85725:CDS 45.0%
ATACAGGCAGAAGAACAAGG+TGG + chr6.1:16667928-16667947 MS.gene85725:CDS 45.0%
ATCTAGGTGGCCAAAAACTG+AGG + chr6.1:16667664-16667683 MS.gene85725:CDS 45.0%
ATGGACCTAAAGGGCCATTA+TGG + chr6.1:16667738-16667757 MS.gene85725:CDS 45.0%
CCATTATGGGAGGAGATTTC+AGG + chr6.1:16667752-16667771 MS.gene85725:CDS 45.0%
CCTGAAATCTCCTCCCATAA+TGG - chr6.1:16667755-16667774 None:intergenic 45.0%
GAAACGACAACAATGGAAGG+GGG + chr6.1:16667017-16667036 MS.gene85725:CDS 45.0%
GAAGAGGAAGAGGAAATGGA+AGG + chr6.1:16667052-16667071 MS.gene85725:CDS 45.0%
GAGGAAACGACAACAATGGA+AGG + chr6.1:16667014-16667033 MS.gene85725:CDS 45.0%
GGAAACGACAACAATGGAAG+GGG + chr6.1:16667016-16667035 MS.gene85725:CDS 45.0%
GGAAGAGGTGGTAAATCAGA+TGG + chr6.1:16666591-16666610 MS.gene85725:CDS 45.0%
GGAGGTTGTTGAGAAACAAG+AGG + chr6.1:16667097-16667116 MS.gene85725:CDS 45.0%
GGGGTGGGAATAGTACTAAT+GGG - chr6.1:16666723-16666742 None:intergenic 45.0%
TATTCTTCCTCATCTCGACG+CGG - chr6.1:16668106-16668125 None:intergenic 45.0%
TGCATGCAAGTTCATCTAGG+TGG + chr6.1:16667651-16667670 MS.gene85725:CDS 45.0%
TGGACCTAAAGGGCCATTAT+GGG + chr6.1:16667739-16667758 MS.gene85725:CDS 45.0%
TTCGGAGGAAACGACAACAA+TGG + chr6.1:16667010-16667029 MS.gene85725:CDS 45.0%
! CAGATTTCTGAAGGTGGCAT+TGG - chr6.1:16665520-16665539 None:intergenic 45.0%
! GCCCTTTAGGTCCATTTTCT+TGG - chr6.1:16667733-16667752 None:intergenic 45.0%
! GCCTGTATAGAGCATCAAGT+TGG - chr6.1:16667916-16667935 None:intergenic 45.0%
! GGTGTAGGTATTGCTGGTAT+TGG - chr6.1:16667461-16667480 None:intergenic 45.0%
! TCAGGTGATGATGAAAGAGG+GGG + chr6.1:16665262-16665281 MS.gene85725:CDS 45.0%
! TGTGTTTGAGTTTGTGGTGG+TGG - chr6.1:16666696-16666715 None:intergenic 45.0%
! TTAGCGAATGAAGATGGTGG+GGG - chr6.1:16666945-16666964 None:intergenic 45.0%
!! GAGGGTGTGGATAGAAGTTT+TGG + chr6.1:16665304-16665323 MS.gene85725:CDS 45.0%
!! GCTGGTATTGGTATTGGCAA+TGG - chr6.1:16667449-16667468 None:intergenic 45.0%
!! GGTATTGGTGGAAAGTGTGT+AGG - chr6.1:16666858-16666877 None:intergenic 45.0%
!! GGTGTGGATAGAAGTTTTGG+TGG + chr6.1:16665307-16665326 MS.gene85725:CDS 45.0%
!! GTTTGAACTTGTTGCGGTGT+AGG - chr6.1:16667476-16667495 None:intergenic 45.0%
!! TGTAGTTGATGGAACAGTGG+TGG - chr6.1:16666802-16666821 None:intergenic 45.0%
!! GAAAAATTAAATTAATAAAT+GGG - chr6.1:16665625-16665644 None:intergenic 5.0%
!! TGAAAAATTAAATTAATAAA+TGG - chr6.1:16665626-16665645 None:intergenic 5.0%
ACATCAGCAGCAGCAACAAC+AGG + chr6.1:16667559-16667578 MS.gene85725:CDS 50.0%
ACCTAAAGGGCCATTATGGG+AGG + chr6.1:16667742-16667761 MS.gene85725:CDS 50.0%
AGCATTCTCAGCATCCTCCA+TGG - chr6.1:16668071-16668090 None:intergenic 50.0%
AGGCAGAAGAACAAGGTGGA+GGG + chr6.1:16667932-16667951 MS.gene85725:CDS 50.0%
CTAGGGGACTCAACAGTGTT+GGG + chr6.1:16665175-16665194 MS.gene85725:CDS 50.0%
GAAGCTGTTGTTGTAGGGGT+GGG - chr6.1:16666738-16666757 None:intergenic 50.0%
GACGGAAGAGGAAGAGGAAA+TGG + chr6.1:16667048-16667067 MS.gene85725:CDS 50.0%
GATGACGAGGACGAAGATGA+AGG + chr6.1:16668148-16668167 MS.gene85725:CDS 50.0%
GGAGGTTGCAAGAGATGCAA+AGG + chr6.1:16667189-16667208 MS.gene85725:CDS 50.0%
GGAGTTGCACAAGAGGTTCT+TGG + chr6.1:16667118-16667137 MS.gene85725:CDS 50.0%
GTGCTAGAGAAGAAGCTTGG+AGG + chr6.1:16667171-16667190 MS.gene85725:CDS 50.0%
GTGGCCAAAAACTGAGGTTG+AGG + chr6.1:16667670-16667689 MS.gene85725:CDS 50.0%
TCCTCCCATAATGGCCCTTT+AGG - chr6.1:16667746-16667765 None:intergenic 50.0%
TGAAGCTGTTGTTGTAGGGG+TGG - chr6.1:16666739-16666758 None:intergenic 50.0%
TGCTGCTGATGTTGTGGCAA+CGG - chr6.1:16667551-16667570 None:intergenic 50.0%
TGTTGCTGCTGCTGATGTTG+TGG - chr6.1:16667557-16667576 None:intergenic 50.0%
! AACGATGATGGCACCGTTGA+TGG + chr6.1:16667982-16668001 MS.gene85725:CDS 50.0%
! GACTCAACAGTGTTGGGAAC+AGG + chr6.1:16665181-16665200 MS.gene85725:CDS 50.0%
! GAGGTGCTAGAGAAGAAGCT+TGG + chr6.1:16667168-16667187 MS.gene85725:CDS 50.0%
! GTTGATGGAACAGTGGTGGT+AGG - chr6.1:16666798-16666817 None:intergenic 50.0%
! TCAACAGTGTTGGGAACAGG+AGG + chr6.1:16665184-16665203 MS.gene85725:CDS 50.0%
! TGGTGGAAAGTGTGTAGGGT+TGG - chr6.1:16666853-16666872 None:intergenic 50.0%
! TGTTGCGGTGTAGGTATTGC+TGG - chr6.1:16667467-16667486 None:intergenic 50.0%
!! AGCTTACCATGATGGTGGTG+GGG + chr6.1:16665231-16665250 MS.gene85725:CDS 50.0%
!! GAGCTTACCATGATGGTGGT+GGG + chr6.1:16665230-16665249 MS.gene85725:CDS 50.0%
!! GGTATTGGCAATGGTACTGC+TGG - chr6.1:16667440-16667459 None:intergenic 50.0%
!! GGTGGTGGTGGTGTTATTGA+AGG - chr6.1:16666681-16666700 None:intergenic 50.0%
!! GTTTGAGTTTGTGGTGGTGG+TGG - chr6.1:16666693-16666712 None:intergenic 50.0%
!! TGATGACGGTGATGATGACG+AGG + chr6.1:16668135-16668154 MS.gene85725:CDS 50.0%
!! TTGAGGTGGTGGTGGTTTGT+TGG - chr6.1:16668197-16668216 None:intergenic 50.0%
CAGGCAGAAGAACAAGGTGG+AGG + chr6.1:16667931-16667950 MS.gene85725:CDS 55.0%
CGAATCGACGGAAGAGGAAG+AGG + chr6.1:16667042-16667061 MS.gene85725:CDS 55.0%
GATGGAACAGTGGTGGTAGG+AGG - chr6.1:16666795-16666814 None:intergenic 55.0%
GCAATGGCCTCCACAACAAC+AGG + chr6.1:16668018-16668037 MS.gene85725:CDS 55.0%
GCAGCAGCAACAACAGGTTC+AGG + chr6.1:16667565-16667584 MS.gene85725:CDS 55.0%
GCAGGAGCTTACCATGATGG+TGG + chr6.1:16665226-16665245 MS.gene85725:CDS 55.0%
GCTAGGGGACTCAACAGTGT+TGG + chr6.1:16665174-16665193 MS.gene85725:CDS 55.0%
GGAGCAGGAGCTTACCATGA+TGG + chr6.1:16665223-16665242 MS.gene85725:CDS 55.0%
GGCAATGGTACTGCTGGTAC+TGG - chr6.1:16667434-16667453 None:intergenic 55.0%
TGCTCCGGCTGAACCATCAA+CGG - chr6.1:16667998-16668017 None:intergenic 55.0%
TGGAAGGGGGATCGAATCGA+CGG + chr6.1:16667030-16667049 MS.gene85725:CDS 55.0%
TTCGTCTTCGGCTTCTTCGG+AGG + chr6.1:16666995-16667014 MS.gene85725:CDS 55.0%
TTGATCCCCCACCACCATCA+TGG - chr6.1:16665240-16665259 None:intergenic 55.0%
! ACAGTGTTGGGAACAGGAGG+AGG + chr6.1:16665187-16665206 MS.gene85725:CDS 55.0%
! GGTGATGATGAAAGAGGGGG+TGG + chr6.1:16665265-16665284 MS.gene85725:CDS 55.0%
! TCAACAACAACAAGCGCCGC+AGG + chr6.1:16667352-16667371 MS.gene85725:CDS 55.0%
!! AACAGCACCGGTCATTCCTC+AGG + chr6.1:16667520-16667539 MS.gene85725:CDS 55.0%
!! AATGGTGCTGCTGCTGGTGT+CGG - chr6.1:16667410-16667429 None:intergenic 55.0%
!! GCTTACCATGATGGTGGTGG+GGG + chr6.1:16665232-16665251 MS.gene85725:CDS 55.0%
!! GGAGCTTACCATGATGGTGG+TGG + chr6.1:16665229-16665248 MS.gene85725:CDS 55.0%
!! GGGGTTGGTGTAGTTTCCTG+CGG - chr6.1:16667371-16667390 None:intergenic 55.0%
!! GGTACTGCTGGTACTGGCAA+TGG - chr6.1:16667428-16667447 None:intergenic 55.0%
!! GGTGGTGGGGGATCAATTTC+AGG + chr6.1:16665244-16665263 MS.gene85725:CDS 55.0%
!! TCGGTGTCGGTGTTGGTGTT+GGG - chr6.1:16667391-16667410 None:intergenic 55.0%
!! TGCGGCGCTTGTTGTTGTTG+AGG - chr6.1:16667353-16667372 None:intergenic 55.0%
GAGGCCTGAGGGAACGATGA+TGG + chr6.1:16667970-16667989 MS.gene85725:CDS 60.0%
GGCGAAGCCTGAGGAATGAC+CGG - chr6.1:16667530-16667549 None:intergenic 60.0%
GGGGATCGAATCGACGGAAG+AGG + chr6.1:16667036-16667055 MS.gene85725:CDS 60.0%
TGGTTCAGCCGGAGCAGCAA+TGG + chr6.1:16668002-16668021 MS.gene85725:CDS 60.0%
TGTGGAGGCCATTGCTGCTC+CGG - chr6.1:16668013-16668032 None:intergenic 60.0%
! GGTGCCATCATCGTTCCCTC+AGG - chr6.1:16667977-16667996 None:intergenic 60.0%
!! ACTGGCAATGGTGCTGCTGC+TGG - chr6.1:16667416-16667435 None:intergenic 60.0%
!! CGGTGTCGGTGTTGGTGTTG+GGG - chr6.1:16667390-16667409 None:intergenic 60.0%
!! GGCACCGTTGATGGTTCAGC+CGG + chr6.1:16667991-16668010 MS.gene85725:CDS 60.0%
!! GTCGGTGTCGGTGTTGGTGT+TGG - chr6.1:16667392-16667411 None:intergenic 60.0%
!! GTCGGTGTTGGTGTTGGGGT+TGG - chr6.1:16667386-16667405 None:intergenic 60.0%
AGCCAGGGCGGATGTGACCA+TGG + chr6.1:16668051-16668070 MS.gene85725:CDS 65.0%
CAGGGCGGATGTGACCATGG+AGG + chr6.1:16668054-16668073 MS.gene85725:CDS 65.0%
CTCCATGGTCACATCCGCCC+TGG - chr6.1:16668056-16668075 None:intergenic 65.0%
GGAACAGGAGGAGGCAGCTC+AGG + chr6.1:16665196-16665215 MS.gene85725:CDS 65.0%
GGAGGAGGCAGCTCAGGAGA+TGG + chr6.1:16665202-16665221 MS.gene85725:CDS 65.0%
GGCAGCTCAGGAGATGGAGC+AGG + chr6.1:16665208-16665227 MS.gene85725:CDS 65.0%
GGTGGCGCCTGTTGTTGTGG+AGG - chr6.1:16668028-16668047 None:intergenic 65.0%
TGATCGGCCGCGTCGAGATG+AGG + chr6.1:16668096-16668115 MS.gene85725:CDS 65.0%
TGTGGCAACGGCGAAGCCTG+AGG - chr6.1:16667539-16667558 None:intergenic 65.0%
! GCTGGTGGCGCCTGTTGTTG+TGG - chr6.1:16668031-16668050 None:intergenic 65.0%
!! GCTGCTGCTGGTGTCGGTGT+CGG - chr6.1:16667404-16667423 None:intergenic 65.0%
!! GCTGGTGTCGGTGTCGGTGT+TGG - chr6.1:16667398-16667417 None:intergenic 65.0%
AACAGGCGCCACCAGCAGCC+AGG + chr6.1:16668035-16668054 MS.gene85725:CDS 70.0%
ACAGGCGCCACCAGCAGCCA+GGG + chr6.1:16668036-16668055 MS.gene85725:CDS 70.0%
ACATCCGCCCTGGCTGCTGG+TGG - chr6.1:16668046-16668065 None:intergenic 70.0%
AGCTGCTGCGTCGAGGCCTG+AGG + chr6.1:16667958-16667977 MS.gene85725:CDS 70.0%
AGGGTGCAGCTGCTGCGTCG+AGG + chr6.1:16667951-16667970 MS.gene85725:CDS 70.0%
GCTGCTGCGTCGAGGCCTGA+GGG + chr6.1:16667959-16667978 MS.gene85725:CDS 70.0%
GTCACATCCGCCCTGGCTGC+TGG - chr6.1:16668049-16668068 None:intergenic 70.0%
! GGCGCCACCAGCAGCCAGGG+CGG + chr6.1:16668039-16668058 MS.gene85725:CDS 80.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 16665172 16668219 16665172 ID=MS.gene85725
chr6.1 mRNA 16665172 16668219 16665172 ID=MS.gene85725.t1;Parent=MS.gene85725
chr6.1 exon 16665172 16665440 16665172 ID=MS.gene85725.t1.exon1;Parent=MS.gene85725.t1
chr6.1 CDS 16665172 16665440 16665172 ID=cds.MS.gene85725.t1;Parent=MS.gene85725.t1
chr6.1 exon 16666500 16668219 16666500 ID=MS.gene85725.t1.exon2;Parent=MS.gene85725.t1
chr6.1 CDS 16666500 16668219 16666500 ID=cds.MS.gene85725.t1;Parent=MS.gene85725.t1
Gene Sequence

>MS.gene85725

ATGCTAGGGGACTCAACAGTGTTGGGAACAGGAGGAGGCAGCTCAGGAGATGGAGCAGGAGCTTACCATGATGGTGGTGGGGGATCAATTTCAGGTGATGATGAAAGAGGGGGTGGAAGTAGTAGAAATGAAGAGGGTGTGGATAGAAGTTTTGGTGGTAATAGATGGCCAAGACAAGAAACAGTTGCTTTGTTGAAGATAAGGTCTGATATGGATGTTGCTTTCAAAGATGCAAGTGTTAAAGGTCCCTTATGGGATGAAGTTTCAAGGTTAGTCAAAAAAAAAAATTCAATTTTTTTCATTTTTTTGATTTTAATTATTTGAGATTGTATTCAACTTGTTAATCCAATGCCACCTTCAGAAATCTGAAAAAAAAATTCACTTTGAGTTCATGTTTTGTATATTAATTTTTTTTTTTTCAATGTGTTTCAATTTTCATTTCACCTCAATCCCATTTATTAATTTAATTTTTCAATTTTTGAGGTATTTTTTACTTGATTCAATGCTACCTTGAGAAATCTGAATTATTGAATGTGTTTCAATTTTGATTTTGATTTCACTTCAATTTCATATTTTTTTTATCTTTGATTTGGACTTGTTTTCATGTAAAGATGAATTTTTTAATTTTTTTTGTTATCATTGAATTATTGAATTGATTATTGAAGTTCCTTCATGTATATTTTTAATTTAAAGATATAAGTACTGTTTATGAAAAATTAAATAATTTAGAGATGTGTGCAAGCATCTCTCTATGACTCTATCTATCTATGATATGATGACTTTGATGTGATTTGCTTTTCTGACATTCATTTTGATACATGTTGTATTTTTACCAAATAAAGTTACAAACTTGAATTGAGTCACCAAAAAGCAAAGATTTTCTTCCTCCCTTATTTCAGTTACCCATTTTTAGTGTTGAAAAATAAAAATATGATGGTAGATTACAAAATCTTTGTTCACACTTTGGTTCATGTGTTTTCCATGAGAAACAAACTTTTTAATTTTTTCAACATTTTACGCTATTTAATTTCTTGTCATTATTAAAAAAAATCTTCACTCCAATTACATCAACTTTGATATATAATAATTTTTTATTCACCTCTCCCTTATCTTAATCACTTAAGTTACTTGCTCCATTTCAATTCTTTGGTTGTCTAAATTGATTTATTTATTTTTATTATTATTATTTTTGACTCAATTCATTTATTATCTTATGCACCAATTAATTGCTTCTTATCCTAAAGATTTAATGTGATAACAAAAAGCGAGAAATTTTATTTTTTTCTTTAATTAATTTTGTCTTTATTTTTGGGTACTTTGGCTAATAGGAAAATGGCAGATCTTGGGTATCAAAGAAACTCCAAAAAATGCAAAGAAAAGTTTGAGAATGTTTACAAATATCACAAGAGAACCAAAGAAGGAAGAGGTGGTAAATCAGATGGTAAAACTTATAGATTCTTTGATCAATTACAAGCCCTTGAAAACAACCCTTCAATGCATCAATCACCTTCAATAACACCACCACCACCACAAACTCAAACACAAACTCCCATTAGTACTATTCCCACCCCTACAACAACAGCTTCATTTCTATCTGCACCTCCATTGTTAAACTCTACACCTCCTACCACCACTGTTCCATCAACTACACTTCCAATTCCAATGTCTTACACACAATCCAACCCTACACACTTTCCACCAATACCATCATCAACAACAACAACAAACCCTACAAATACTAATAACCCTCTTCCTCAAATTACTATTACACCCCCACCATCTTCATTCGCTAATATTTCAGCGGATTTCTTCTCGAATTCAAGTTCGTCTTCGGCTTCTTCGGAGGAAACGACAACAATGGAAGGGGGATCGAATCGACGGAAGAGGAAGAGGAAATGGAAGGATTTCTTTGAGAGACTAATGAAGGAGGTTGTTGAGAAACAAGAGGAGTTGCACAAGAGGTTCTTGGAAGCTATAGAGAAAAGAGAAAGAGAGAGAGGTGCTAGAGAAGAAGCTTGGAGGTTGCAAGAGATGCAAAGGATTAATAGAGAAAGGGAAATTCTTGCTCAAGAGAGATCTATTGCTGCTACTAAAGATGCTGCTGTTATGGCTTTTTTGCAGAAGATTGCTGAACAACAAAATCTTGTTCCTCCTGTATTGAACAACAACACCGTCATTCCTCAACAACAACAAGCGCCGCAGGAAACTACACCAACCCCAACACCAACACCGACACCGACACCAGCAGCAGCACCATTGCCAGTACCAGCAGTACCATTGCCAATACCAATACCAGCAATACCTACACCGCAACAAGTTCAAACTCCAACTGTAACAGTGCAACAACAAACAGCACCGGTCATTCCTCAGGCTTCGCCGTTGCCACAACATCAGCAGCAGCAACAACAGGTTCAGGTACAACAGCAGCAAGTGACGAATATGGAAGTTGCGAAAAGTGACAACAATGGTGAGAGTATGATGCATGCAAGTTCATCTAGGTGGCCAAAAACTGAGGTTGAGGCATTGATAAAGCTGAGAACAACTCTTGATATGAAGTACCAAGAAAATGGACCTAAAGGGCCATTATGGGAGGAGATTTCAGGTTTGATGAAGAAAATGGGGTACAATAGGAATGCAAAGAGGTGCAAAGAGAAATGGGAGAACATCAACAAGTATTTCAAGAAAGTGAAGGAAAGCAACAAGAAGAGGCCAGAAGATTCAAAGACATGTCCTTACTTTCACCAACTTGATGCTCTATACAGGCAGAAGAACAAGGTGGAGGGTGCAGCTGCTGCGTCGAGGCCTGAGGGAACGATGATGGCACCGTTGATGGTTCAGCCGGAGCAGCAATGGCCTCCACAACAACAGGCGCCACCAGCAGCCAGGGCGGATGTGACCATGGAGGATGCTGAGAATGCTGAGAATGATCGGCCGCGTCGAGATGAGGAAGAATATTATGACGATGATGACGGTGATGATGACGAGGACGAAGATGAAGGTAGTGACAATTATGAAGTGGTTGCCAACAAACCACCACCACCTCAATAA
Protein sequence

>MS.gene85725.t1

MLGDSTVLGTGGGSSGDGAGAYHDGGGGSISGDDERGGGSSRNEEGVDRSFGGNRWPRQETVALLKIRSDMDVAFKDASVKGPLWDEVSRKMADLGYQRNSKKCKEKFENVYKYHKRTKEGRGGKSDGKTYRFFDQLQALENNPSMHQSPSITPPPPQTQTQTPISTIPTPTTTASFLSAPPLLNSTPPTTTVPSTTLPIPMSYTQSNPTHFPPIPSSTTTTNPTNTNNPLPQITITPPPSSFANISADFFSNSSSSSASSEETTTMEGGSNRRKRKRKWKDFFERLMKEVVEKQEELHKRFLEAIEKRERERGAREEAWRLQEMQRINREREILAQERSIAATKDAAVMAFLQKIAEQQNLVPPVLNNNTVIPQQQQAPQETTPTPTPTPTPTPAAAPLPVPAVPLPIPIPAIPTPQQVQTPTVTVQQQTAPVIPQASPLPQHQQQQQQVQVQQQQVTNMEVAKSDNNGESMMHASSSRWPKTEVEALIKLRTTLDMKYQENGPKGPLWEEISGLMKKMGYNRNAKRCKEKWENINKYFKKVKESNKKRPEDSKTCPYFHQLDALYRQKNKVEGAAAASRPEGTMMAPLMVQPEQQWPPQQQAPPAARADVTMEDAENAENDRPRRDEEEYYDDDDGDDDEDEDEGSDNYEVVANKPPPPQ