Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85782.t1 | PNY05171.1 | 96.4 | 110 | 4 | 0 | 1 | 110 | 311 | 420 | 8.80E-55 | 222.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85782.t1 | A0A2K3NQ66 | 96.4 | 110 | 4 | 0 | 1 | 110 | 311 | 420 | 6.4e-55 | 222.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85782.t1 | MTR_0349s0030 | 96.364 | 110 | 4 | 0 | 1 | 110 | 184 | 293 | 8.92e-76 | 226 |
| MS.gene85782.t1 | MTR_6g074905 | 96.364 | 110 | 4 | 0 | 1 | 110 | 1064 | 1173 | 2.02e-71 | 230 |
| MS.gene85782.t1 | MTR_6g074640 | 89.216 | 102 | 10 | 1 | 9 | 110 | 139 | 239 | 1.74e-60 | 185 |
| MS.gene85782.t1 | MTR_6g074670 | 92.045 | 88 | 7 | 0 | 1 | 88 | 35 | 122 | 6.36e-56 | 170 |
| MS.gene85782.t1 | MTR_5g082700 | 54.630 | 108 | 49 | 0 | 1 | 108 | 605 | 712 | 1.32e-37 | 134 |
| MS.gene85782.t1 | MTR_6g074835 | 93.548 | 62 | 4 | 0 | 1 | 62 | 63 | 124 | 3.32e-37 | 122 |
| MS.gene85782.t1 | MTR_6g074870 | 90.566 | 53 | 5 | 0 | 1 | 53 | 295 | 347 | 1.09e-29 | 108 |
| MS.gene85782.t1 | MTR_6g074480 | 93.750 | 48 | 3 | 0 | 63 | 110 | 30 | 77 | 3.35e-28 | 98.2 |
| MS.gene85782.t1 | MTR_8g069620 | 91.837 | 49 | 4 | 0 | 1 | 49 | 153 | 201 | 1.56e-26 | 97.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85782.t1 | AT1G73450 | 81.818 | 110 | 20 | 0 | 1 | 110 | 1043 | 1152 | 4.87e-62 | 204 |
| MS.gene85782.t1 | AT1G73460 | 81.818 | 110 | 20 | 0 | 1 | 110 | 1060 | 1169 | 8.62e-62 | 203 |
| MS.gene85782.t1 | AT1G73460 | 81.818 | 110 | 20 | 0 | 1 | 110 | 1060 | 1169 | 8.62e-62 | 203 |
| MS.gene85782.t1 | AT3G17750 | 84.545 | 110 | 17 | 0 | 1 | 110 | 1029 | 1138 | 6.13e-61 | 201 |
| MS.gene85782.t1 | AT4G03175 | 52.174 | 92 | 44 | 0 | 11 | 102 | 2 | 93 | 4.69e-34 | 113 |
| MS.gene85782.t1 | AT2G40120 | 50.980 | 102 | 50 | 0 | 1 | 102 | 463 | 564 | 9.53e-33 | 119 |
Find 0 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTCAATTTCAGAAATTTTA+GGG | + | 42462:23407-23426 | MS.gene85782:intergenic | 20.0% |
| !!! | ATTTTTTGTGAAGTACTTAT+AGG | + | 42462:23299-23318 | MS.gene85782:intergenic | 20.0% |
| !!! | TAGTCAATTTCAGAAATTTT+AGG | + | 42462:23408-23427 | MS.gene85782:intergenic | 20.0% |
| ! | AAAATCATATGCTTTATGAG+AGG | - | 42462:23314-23333 | MS.gene85782:CDS | 25.0% |
| ! | AACACAATCGTATGATAATA+TGG | + | 42462:23596-23615 | MS.gene85782:intergenic | 25.0% |
| ! | ACACAATCGTATGATAATAT+GGG | + | 42462:23595-23614 | MS.gene85782:intergenic | 25.0% |
| ! | ATACAATCGTATCATAACAT+AGG | + | 42462:23659-23678 | MS.gene85782:intergenic | 25.0% |
| ! | ATTGTCTTCAAAAAGCAAAA+TGG | - | 42462:23372-23391 | MS.gene85782:intron | 25.0% |
| ! | TTGTCTTCAAAAAGCAAAAT+GGG | - | 42462:23373-23392 | MS.gene85782:intron | 25.0% |
| !! | TTTTCCATGATAATTAAACG+TGG | + | 42462:23704-23723 | MS.gene85782:intergenic | 25.0% |
| GTATGTTATAATTGTTGCCA+TGG | - | 42462:23675-23694 | MS.gene85782:intron | 30.0% | |
| TAAGCTGTTAATCCAAACAT+GGG | + | 42462:23450-23469 | MS.gene85782:intergenic | 30.0% | |
| TTAAGCTGTTAATCCAAACA+TGG | + | 42462:23451-23470 | MS.gene85782:intergenic | 30.0% | |
| TTTCCATAAACTCTTTCGAA+CGG | - | 42462:23558-23577 | MS.gene85782:intron | 30.0% | |
| AAAGTATGCTAGCAAAAGGA+CGG | - | 42462:23269-23288 | MS.gene85782:CDS | 35.0% | |
| AACATAGGCATGTTTCGATT+CGG | + | 42462:23644-23663 | MS.gene85782:intergenic | 35.0% | |
| TATGCTTTATGAGAGGAATC+AGG | - | 42462:23321-23340 | MS.gene85782:CDS | 35.0% | |
| TCCATGATAATTAAACGTGG+AGG | + | 42462:23701-23720 | MS.gene85782:intergenic | 35.0% | |
| TCTTGTATATTGCCCATGTT+TGG | - | 42462:23435-23454 | MS.gene85782:intron | 35.0% | |
| TTCCAAAGAAGACATCTTTG+AGG | - | 42462:23783-23802 | MS.gene85782:intron | 35.0% | |
| ! | GATACTGTGTATTGATATGC+AGG | - | 42462:23736-23755 | MS.gene85782:intron | 35.0% |
| ! | TTTAGAACTTCAGATGCAGA+AGG | + | 42462:23884-23903 | MS.gene85782:intergenic | 35.0% |
| !! | AAGATGATATCGGTTCGTAA+GGG | + | 42462:23925-23944 | MS.gene85782:intergenic | 35.0% |
| !! | AAGATGTCTTCTTTGGAATC+AGG | + | 42462:23781-23800 | MS.gene85782:intergenic | 35.0% |
| !! | TGTTTCGATTCGGTTATTTG+AGG | + | 42462:23634-23653 | MS.gene85782:intergenic | 35.0% |
| AAGTATGCTAGCAAAAGGAC+GGG | - | 42462:23270-23289 | MS.gene85782:CDS | 40.0% | |
| ACATTACTTGCCAGGGTAAT+CGG | - | 42462:23226-23245 | MS.gene85782:CDS | 40.0% | |
| AGACCGTTCGAAAGAGTTTA+TGG | + | 42462:23564-23583 | MS.gene85782:intergenic | 40.0% | |
| ATAATTAAACGTGGAGGCCA+TGG | + | 42462:23695-23714 | MS.gene85782:intergenic | 40.0% | |
| CAACAAAGTCAATGAAGCCT+TGG | + | 42462:23829-23848 | MS.gene85782:intergenic | 40.0% | |
| GCCTCCACGTTTAATTATCA+TGG | - | 42462:23697-23716 | MS.gene85782:intron | 40.0% | |
| GCTAGCATACTTTGACCAAT+TGG | + | 42462:23262-23281 | MS.gene85782:intergenic | 40.0% | |
| GGTCAAAGTATGCTAGCAAA+AGG | - | 42462:23265-23284 | MS.gene85782:CDS | 40.0% | |
| TCTTTCGAACGGTCTCATAA+GGG | - | 42462:23569-23588 | MS.gene85782:intron | 40.0% | |
| TGAGGCATAGATTACCGATA+GGG | - | 42462:23801-23820 | MS.gene85782:intron | 40.0% | |
| TGCCTCAAAGATGTCTTCTT+TGG | + | 42462:23788-23807 | MS.gene85782:intergenic | 40.0% | |
| TTGAGGCATAGATTACCGAT+AGG | - | 42462:23800-23819 | MS.gene85782:intron | 40.0% | |
| TTGATATGCAGGAAAGCAAC+AGG | - | 42462:23747-23766 | MS.gene85782:intron | 40.0% | |
| ! | TCGTAAGGGTATGATAACCA+GGG | + | 42462:23911-23930 | MS.gene85782:intergenic | 40.0% |
| ! | TTCGTAAGGGTATGATAACC+AGG | + | 42462:23912-23931 | MS.gene85782:intergenic | 40.0% |
| !! | CAAGATGATATCGGTTCGTA+AGG | + | 42462:23926-23945 | MS.gene85782:intergenic | 40.0% |
| CATTACTTGCCAGGGTAATC+GGG | - | 42462:23227-23246 | MS.gene85782:CDS | 45.0% | |
| CTCTTTCGAACGGTCTCATA+AGG | - | 42462:23568-23587 | MS.gene85782:intron | 45.0% | |
| CTTTGTTGCTCATTTGCTCG+AGG | - | 42462:23841-23860 | MS.gene85782:CDS | 45.0% | |
| GAGGCATAGATTACCGATAG+GGG | - | 42462:23802-23821 | MS.gene85782:intron | 45.0% | |
| ! | CGTAAGGGTATGATAACCAG+GGG | + | 42462:23910-23929 | MS.gene85782:intergenic | 45.0% |
| !! | TATAATTAAAGATAAAATAA+AGG | - | 42462:23526-23545 | MS.gene85782:intron | 5.0% |
| ACCCTGGCAAGTAATGTTGC+AGG | + | 42462:23223-23242 | MS.gene85782:intergenic | 50.0% | |
| AGATTACCGATAGGGGACCA+AGG | - | 42462:23809-23828 | MS.gene85782:CDS | 50.0% | |
| ATCGGGATCATCGGTCCAAT+TGG | - | 42462:23244-23263 | MS.gene85782:CDS | 50.0% | |
| ATGAAGCCTTGGTCCCCTAT+CGG | + | 42462:23818-23837 | MS.gene85782:intergenic | 50.0% | |
| ATGCAGAAGGTCGCTTCTTC+GGG | + | 42462:23871-23890 | MS.gene85782:intergenic | 50.0% | |
| CCCTGCAACATTACTTGCCA+GGG | - | 42462:23219-23238 | MS.gene85782:CDS | 50.0% | |
| CCCTGGCAAGTAATGTTGCA+GGG | + | 42462:23222-23241 | MS.gene85782:intergenic | 50.0% | |
| CCTGGCAAGTAATGTTGCAG+GGG | + | 42462:23221-23240 | MS.gene85782:intergenic | 50.0% | |
| GATGCAGAAGGTCGCTTCTT+CGG | + | 42462:23872-23891 | MS.gene85782:intergenic | 50.0% | |
| !! | CTGAAGTTCTAAAGCACCCC+TGG | - | 42462:23891-23910 | MS.gene85782:CDS | 50.0% |
| ACCGATGATCCCGATTACCC+TGG | + | 42462:23239-23258 | MS.gene85782:intergenic | 55.0% | |
| CCCCTGCAACATTACTTGCC+AGG | - | 42462:23218-23237 | MS.gene85782:CDS | 55.0% | |
| GCCAGGGTAATCGGGATCAT+CGG | - | 42462:23235-23254 | MS.gene85782:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 42462 | gene | 23199 | 23946 | 23199 | ID=MS.gene85782 |
| 42462 | mRNA | 23199 | 23946 | 23199 | ID=MS.gene85782.t1;Parent=MS.gene85782 |
| 42462 | exon | 23803 | 23946 | 23803 | ID=MS.gene85782.t1.exon1;Parent=MS.gene85782.t1 |
| 42462 | CDS | 23803 | 23946 | 23803 | ID=cds.MS.gene85782.t1;Parent=MS.gene85782.t1 |
| 42462 | exon | 23199 | 23387 | 23199 | ID=MS.gene85782.t1.exon2;Parent=MS.gene85782.t1 |
| 42462 | CDS | 23199 | 23387 | 23199 | ID=cds.MS.gene85782.t1;Parent=MS.gene85782.t1 |
| Gene Sequence |
| Protein sequence |
