AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85941

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85941.t1 MTR_2g104530 87.108 287 26 4 1 285 3 280 5.93e-175 484
MS.gene85941.t1 MTR_2g104500 64.333 300 60 11 1 281 3 274 2.58e-117 338
MS.gene85941.t1 MTR_2g104550 42.712 295 139 10 8 279 25 312 1.57e-61 197
MS.gene85941.t1 MTR_2g104490 41.017 295 144 9 4 277 21 306 4.17e-55 181
MS.gene85941.t1 MTR_4g009540 45.607 239 118 5 44 278 100 330 4.44e-55 181
MS.gene85941.t1 MTR_2g104490 41.544 272 129 9 8 258 25 287 4.97e-49 164
MS.gene85941.t1 MTR_4g009110 41.667 240 130 5 44 278 115 349 4.11e-48 164
MS.gene85941.t1 MTR_4g009540 45.205 219 108 5 44 258 100 310 7.80e-47 159
MS.gene85941.t1 MTR_8g009020 42.460 252 128 7 37 278 78 322 3.80e-46 158
MS.gene85941.t1 MTR_2g104560 40.642 187 100 4 95 278 46 224 7.70e-39 136
MS.gene85941.t1 MTR_2g104590 42.778 180 82 3 95 274 61 219 6.18e-35 126
MS.gene85941.t1 MTR_5g014520 36.864 236 130 5 47 274 101 325 2.17e-33 124
MS.gene85941.t1 MTR_5g014600 36.757 185 107 3 104 278 150 334 3.22e-32 121
MS.gene85941.t1 MTR_6g047570 33.473 239 147 4 39 274 98 327 5.01e-32 121
MS.gene85941.t1 MTR_8g069740 33.818 275 148 7 5 257 3 265 1.50e-31 118
MS.gene85941.t1 MTR_7g080780 32.453 265 157 3 14 278 73 315 1.65e-31 119
MS.gene85941.t1 MTR_7g080780 32.453 265 157 3 14 278 86 328 2.24e-31 119
MS.gene85941.t1 MTR_2g104650 33.942 274 165 4 5 274 3 264 7.35e-30 114
MS.gene85941.t1 MTR_4g097940 32.830 265 158 7 18 277 75 324 1.02e-29 115
MS.gene85941.t1 MTR_4g092700 33.068 251 156 6 29 277 89 329 2.01e-29 114
MS.gene85941.t1 MTR_8g009010 34.884 258 115 6 26 276 58 269 8.69e-29 112
MS.gene85941.t1 MTR_5g014640 33.486 218 130 3 70 278 112 323 1.83e-28 111
MS.gene85941.t1 MTR_4g009510 38.824 170 79 3 114 277 53 203 1.85e-28 108
MS.gene85941.t1 MTR_2g010450 36.628 172 105 2 104 274 178 346 6.66e-28 110
MS.gene85941.t1 MTR_4g097920 32.453 265 159 7 18 277 60 309 2.10e-27 108
MS.gene85941.t1 MTR_0250s0040 36.628 172 104 3 104 274 89 256 2.30e-27 107
MS.gene85941.t1 MTR_4g097920 32.453 265 159 7 18 277 75 324 3.37e-27 108
MS.gene85941.t1 MTR_0011s0260 35.838 173 106 3 104 275 184 352 4.78e-27 108
MS.gene85941.t1 MTR_7g451650 39.506 162 72 4 119 274 51 192 6.36e-27 104
MS.gene85941.t1 MTR_7g050350 40.541 148 63 3 133 274 2 130 9.39e-27 102
MS.gene85941.t1 MTR_7g451650 40.541 148 63 3 133 274 2 130 9.39e-27 102
MS.gene85941.t1 MTR_0011s0210 35.260 173 107 3 104 275 184 352 1.34e-26 107
MS.gene85941.t1 MTR_0246s0020 32.843 204 126 5 80 274 169 370 2.19e-26 107
MS.gene85941.t1 MTR_4g066460 32.520 246 135 8 44 278 99 324 5.90e-26 104
MS.gene85941.t1 MTR_5g014560 36.994 173 98 2 106 274 134 299 1.58e-25 103
MS.gene85941.t1 MTR_4g067010 33.714 175 110 3 104 278 148 316 3.62e-25 102
MS.gene85941.t1 MTR_0246s0050 28.502 207 130 3 75 274 117 312 2.72e-24 100
MS.gene85941.t1 MTR_4g098035 28.502 207 130 3 75 274 138 333 3.13e-24 100
MS.gene85941.t1 MTR_4g097950 34.188 234 132 6 59 278 24 249 1.85e-23 96.7
MS.gene85941.t1 MTR_4g009420 36.646 161 80 3 114 274 54 192 1.90e-23 95.1
MS.gene85941.t1 MTR_7g451650 40.541 148 62 4 121 262 53 180 4.33e-23 94.0
MS.gene85941.t1 MTR_0246s0020 32.663 199 122 6 80 268 169 365 1.61e-22 95.9
MS.gene85941.t1 MTR_4g066380 32.338 201 123 3 80 274 124 317 3.04e-20 89.4
MS.gene85941.t1 MTR_4g009420 36.913 149 72 3 114 262 54 180 1.44e-19 85.1
MS.gene85941.t1 MTR_5g014550 35.714 126 78 1 132 257 53 175 1.23e-13 68.6
MS.gene85941.t1 MTR_8g098275 27.011 174 104 3 104 254 309 482 6.50e-13 68.9
MS.gene85941.t1 MTR_8g098275 27.011 174 104 3 104 254 402 575 7.50e-13 68.9
MS.gene85941.t1 MTR_8g098275 27.011 174 104 3 104 254 245 418 7.54e-13 68.6
MS.gene85941.t1 MTR_1g072320 28.488 172 92 4 80 237 455 609 4.24e-12 66.6
MS.gene85941.t1 MTR_8g024790 27.313 227 145 6 25 242 213 428 3.30e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85941.t1 AT2G22760 37.433 187 112 4 95 278 111 295 1.05e-30 117
MS.gene85941.t1 AT4G37850 36.041 197 123 3 83 279 133 326 4.30e-30 115
MS.gene85941.t1 AT4G37850 36.041 197 123 3 83 279 133 326 4.30e-30 115
MS.gene85941.t1 AT2G22760 37.952 166 99 3 95 258 111 274 7.97e-27 106
MS.gene85941.t1 AT2G22770 32.787 183 111 2 104 274 133 315 3.47e-24 100
MS.gene85941.t1 AT2G22750 35.938 192 98 3 95 273 118 297 4.71e-24 99.4
MS.gene85941.t1 AT2G22750 35.938 192 98 3 95 273 146 325 7.64e-24 99.4
MS.gene85941.t1 AT2G22750 35.938 192 98 3 95 273 142 321 8.84e-24 99.0
MS.gene85941.t1 AT2G22750 35.938 192 97 4 95 273 118 296 1.20e-22 95.5
MS.gene85941.t1 AT2G22750 35.938 192 97 4 95 273 146 324 2.02e-22 95.1
MS.gene85941.t1 AT4G09820 35.294 153 69 6 94 242 351 477 3.39e-14 72.8
MS.gene85941.t1 AT1G63650 29.167 168 96 2 87 235 389 552 1.19e-11 65.5
MS.gene85941.t1 AT1G63650 29.167 168 96 2 87 235 389 552 1.19e-11 65.5
MS.gene85941.t1 AT1G63650 29.167 168 96 2 87 235 389 552 1.19e-11 65.5

Find 59 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTATTGTGAAGTATTAT+TGG 0.152976 2.1:+1232369 None:intergenic
AGTATTGATCTTCTCTTCTT+TGG 0.201867 2.1:+1233116 None:intergenic
ATCAATTGTGAGAAGGAAAA+TGG 0.286777 2.1:-1232225 MS.gene85941:CDS
TGGCATTCAACTCAAAATAT+TGG 0.318480 2.1:-1232205 MS.gene85941:CDS
GACACTTTCACTTTGATATC+AGG 0.319665 2.1:+1232270 None:intergenic
ACCAATAATACTTCACAATA+AGG 0.330869 2.1:-1232370 MS.gene85941:CDS
CCGTTTGGCAATTCAACTCT+TGG 0.391384 2.1:-1232135 MS.gene85941:CDS
TGATTCAAAGCTTATATTTG+AGG 0.412613 2.1:+1233161 None:intergenic
AATCTTCGTGGCAGCGGTGG+CGG 0.416309 2.1:+1233293 None:intergenic
AGCACCAGCGTATTGCCGTT+TGG 0.418325 2.1:-1232150 MS.gene85941:CDS
TTTAGGGTAATAAGGTGGTT+TGG 0.419274 2.1:+1233194 None:intergenic
CTTCCCTGGTATTGTGGCTA+TGG 0.426831 2.1:+1233218 None:intergenic
GCGGCTCCGGCTACTATAGA+TGG 0.428100 2.1:+1233312 None:intergenic
ATATCAGGAAGCAATTCATT+TGG 0.433496 2.1:+1232285 None:intergenic
CGTGGCAGCGGTGGCGGCTC+CGG 0.438466 2.1:+1233299 None:intergenic
CGCCAAGGAATTCACCATCT+TGG 0.480758 2.1:+1233352 None:intergenic
GACAAGGCCTCTATAGTTCA+AGG 0.488754 2.1:-1232468 MS.gene85941:CDS
CGGCGAGCTTGCAATCTTCG+TGG 0.489491 2.1:+1233281 None:intergenic
GCTTGCAATCTTCGTGGCAG+CGG 0.490965 2.1:+1233287 None:intergenic
TTTGGACTTCCCTGGTATTG+TGG 0.495026 2.1:+1233212 None:intergenic
CACCAAGATGGTGAATTCCT+TGG 0.497019 2.1:-1233354 MS.gene85941:CDS
TGGATCTAGCGGATAACATA+AGG 0.501788 2.1:-1230186 MS.gene85941:CDS
CACGAAGATTGCAAGCTCGC+CGG 0.503233 2.1:-1233280 MS.gene85941:CDS
CACAATAAGGAAAATTATTG+TGG 0.503978 2.1:-1232357 MS.gene85941:CDS
AATTCCATAGCCACAATACC+AGG 0.516400 2.1:-1233222 MS.gene85941:CDS
TGGCATTACTATCATCGCTC+AGG 0.520862 2.1:-1232115 MS.gene85941:intron
TGAATTCCTTGGCGACTACA+TGG 0.522050 2.1:-1233343 MS.gene85941:CDS
AGCTTTGAATCAAAAGCAAA+TGG 0.523173 2.1:-1233150 MS.gene85941:CDS
ACACACTGGCAGAGAGGAGA+AGG 0.529594 2.1:-1233068 MS.gene85941:CDS
ATAAGGAAACTGTTGGTGAA+TGG 0.532126 2.1:-1230169 MS.gene85941:CDS
TAAGGTGGTTTGGACTTCCC+TGG 0.533936 2.1:+1233204 None:intergenic
GAAGAGAGAATGCTAGTGCT+AGG 0.534839 2.1:+1233249 None:intergenic
CAAGGAATTCACCATCTTGG+TGG 0.540396 2.1:+1233355 None:intergenic
GATAGCGCCTTGAACTATAG+AGG 0.545601 2.1:+1232461 None:intergenic
TGTAGATGGGTGATGCATAC+AGG 0.548489 2.1:-1230219 MS.gene85941:intron
AAGATTCATTTCACTAGATG+TGG 0.551691 2.1:+1232323 None:intergenic
TTGTTATACAGGATATGGAT+TGG 0.552653 2.1:-1233392 None:intergenic
AAACGGCAATACGCTGGTGC+TGG 0.555816 2.1:+1232152 None:intergenic
TGCAATCTTCGTGGCAGCGG+TGG 0.556907 2.1:+1233290 None:intergenic
GAATCAAAAGCAAATGGCAA+AGG 0.572843 2.1:-1233144 MS.gene85941:CDS
CTAGTGCTAGGAAGCAGCTC+CGG 0.573968 2.1:+1233261 None:intergenic
CCAAGAGTTGAATTGCCAAA+CGG 0.574130 2.1:+1232135 None:intergenic
CAACAACCATCTATAGTAGC+CGG 0.584716 2.1:-1233318 MS.gene85941:CDS
TTTATTACCTTCGTTGAGCG+AGG 0.586963 2.1:+1233000 None:intergenic
GAATCGATGTGCCACCAAGA+TGG 0.596035 2.1:-1233366 MS.gene85941:CDS
CATTTACATCAATTGTGAGA+AGG 0.601314 2.1:-1232232 MS.gene85941:CDS
GGATAACATAAGGAAACTGT+TGG 0.609049 2.1:-1230176 MS.gene85941:CDS
TATAATTCCTCGCTCAACGA+AGG 0.620367 2.1:-1233007 MS.gene85941:intron
TTCATTTCACTAGATGTGGT+AGG 0.630513 2.1:+1232327 None:intergenic
GAAGTACAACAAAACAAGAG+AGG 0.639060 2.1:-1232399 MS.gene85941:CDS
TGCATACAGGATGACGATGA+TGG 0.639126 2.1:-1230206 MS.gene85941:CDS
AAAGACTCTATTTCACACAC+TGG 0.641330 2.1:-1233082 MS.gene85941:CDS
GTTGTGCCATGTAGTCGCCA+AGG 0.642754 2.1:+1233337 None:intergenic
TGATATCAAAGTGAAAGTGT+CGG 0.646805 2.1:-1232268 MS.gene85941:CDS
ATTGCCAAACGGCAATACGC+TGG 0.648172 2.1:+1232146 None:intergenic
GATGACGATGATGGATCTAG+CGG 0.664627 2.1:-1230197 MS.gene85941:CDS
TATTTCACACACTGGCAGAG+AGG 0.667005 2.1:-1233074 MS.gene85941:CDS
ATTCCATAGCCACAATACCA+GGG 0.679504 2.1:-1233221 MS.gene85941:CDS
TCAAGGCGCTATCAATTACG+TGG 0.680617 2.1:-1232451 MS.gene85941:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATGAAATTAAAATAAAA+TGG + chr2.1:1232406-1232425 None:intergenic 10.0%
!! ACATGAAATTAAAATAAAAT+GGG + chr2.1:1232405-1232424 None:intergenic 10.0%
!! TATATATATATATATAGACA+TGG - chr2.1:1232676-1232695 MS.gene85941:intron 10.0%
!! AAATGTATTAATTAATGCAA+GGG + chr2.1:1230586-1230605 None:intergenic 15.0%
!! CTAGAAAAATAAAAAATCAT+TGG - chr2.1:1233152-1233171 MS.gene85941:CDS 15.0%
!! GAAAAAAAATCAAAGAAAAA+TGG + chr2.1:1231883-1231902 None:intergenic 15.0%
!!! AATTTTATCACAATGAATTT+TGG + chr2.1:1232995-1233014 None:intergenic 15.0%
!!! TTATTTTAATTTCATGTTCT+GGG - chr2.1:1232408-1232427 MS.gene85941:CDS 15.0%
!!! TTTATTTTAATTTCATGTTC+TGG - chr2.1:1232407-1232426 MS.gene85941:CDS 15.0%
!!! TTTTTTTTATCATATCTAAC+TGG - chr2.1:1233126-1233145 MS.gene85941:CDS 15.0%
!! AATGTATTAATTAATGCAAG+GGG + chr2.1:1230585-1230604 None:intergenic 20.0%
!! AGAAAGTAATTTCACAAAAT+TGG + chr2.1:1231987-1232006 None:intergenic 20.0%
!! GAAATGTATTAATTAATGCA+AGG + chr2.1:1230587-1230606 None:intergenic 20.0%
!! TAATAAGATTGTGTATCTAA+TGG - chr2.1:1232513-1232532 MS.gene85941:intron 20.0%
!! TTTCCCTAAAAAGTTAATAT+AGG + chr2.1:1230765-1230784 None:intergenic 20.0%
!!! ACTCCTATATTAACTTTTTA+GGG - chr2.1:1230759-1230778 MS.gene85941:intron 20.0%
!!! TACTCCTATATTAACTTTTT+AGG - chr2.1:1230758-1230777 MS.gene85941:intron 20.0%
!!! TATTTTGTTATAAAGAGAGT+GGG + chr2.1:1231964-1231983 None:intergenic 20.0%
!!! TTTGAGAATTTTTTTTCAGT+GGG - chr2.1:1232195-1232214 MS.gene85941:CDS 20.0%
! AAAACTTCAAAAAAGAGTGA+TGG - chr2.1:1231101-1231120 MS.gene85941:intron 25.0%
! AAAGAGTTTGGTATTTCATT+AGG + chr2.1:1231908-1231927 None:intergenic 25.0%
! AAATGAAAAAGCTATCATTG+TGG - chr2.1:1232817-1232836 MS.gene85941:intron 25.0%
! AAGATTCATTGAATCATAGA+TGG + chr2.1:1230874-1230893 None:intergenic 25.0%
! ACCAATAATACTTCACAATA+AGG - chr2.1:1231158-1231177 MS.gene85941:intron 25.0%
! ATGTATTAATTAATGCAAGG+GGG + chr2.1:1230584-1230603 None:intergenic 25.0%
! CACAATAAGGAAAATTATTG+TGG - chr2.1:1231171-1231190 MS.gene85941:intron 25.0%
! GAAGAATAAGAAGATGATTA+AGG + chr2.1:1231940-1231959 None:intergenic 25.0%
! TGATTCAAAGCTTATATTTG+AGG + chr2.1:1230370-1230389 None:intergenic 25.0%
! TTAATACACAATCCAAATCT+CGG - chr2.1:1230892-1230911 MS.gene85941:intron 25.0%
! TTTAATAGTCATGATACGAT+AGG - chr2.1:1231590-1231609 MS.gene85941:intron 25.0%
! TTTGATGTAGTGTTAAAGTA+GGG + chr2.1:1231461-1231480 None:intergenic 25.0%
!! CAAAAAAATTCTAGTGTTGA+AGG - chr2.1:1232486-1232505 MS.gene85941:intron 25.0%
!! TCCTTATTGTGAAGTATTAT+TGG + chr2.1:1231162-1231181 None:intergenic 25.0%
!! TTTTGATGTAGTGTTAAAGT+AGG + chr2.1:1231462-1231481 None:intergenic 25.0%
!!! CTTTGAGAATTTTTTTTCAG+TGG - chr2.1:1232194-1232213 MS.gene85941:CDS 25.0%
!!! GGGAAAAAAAGAGAATTTTT+TGG - chr2.1:1230779-1230798 MS.gene85941:intron 25.0%
!!! GTATTTTGTTATAAAGAGAG+TGG + chr2.1:1231965-1231984 None:intergenic 25.0%
!!! TAACATGGTTTTACTTGATT+TGG + chr2.1:1231842-1231861 None:intergenic 25.0%
!!! TGTTATTGACTCGAATTTAA+TGG - chr2.1:1231005-1231024 MS.gene85941:intron 25.0%
!!! TTGGCTCTTTTAAATTCTAT+TGG + chr2.1:1232976-1232995 None:intergenic 25.0%
AAAATAAAATGGGTGCTTGA+CGG + chr2.1:1232395-1232414 None:intergenic 30.0%
AACATTAACTTTCACATGGT+GGG + chr2.1:1231651-1231670 None:intergenic 30.0%
AAGATTCATTTCACTAGATG+TGG + chr2.1:1231208-1231227 None:intergenic 30.0%
AATCTATGTGGAATTTAGCA+TGG + chr2.1:1232580-1232599 None:intergenic 30.0%
AGGAATTGAAGAAAAGAGTT+TGG + chr2.1:1231920-1231939 None:intergenic 30.0%
ATATCAGGAAGCAATTCATT+TGG + chr2.1:1231246-1231265 None:intergenic 30.0%
ATCAATTGTGAGAAGGAAAA+TGG - chr2.1:1231303-1231322 MS.gene85941:intron 30.0%
ATCTATGTGGAATTTAGCAT+GGG + chr2.1:1232579-1232598 None:intergenic 30.0%
CATAAAGTCATCCATATTAG+AGG - chr2.1:1233215-1233234 MS.gene85941:CDS 30.0%
CATTTACATCAATTGTGAGA+AGG - chr2.1:1231296-1231315 MS.gene85941:intron 30.0%
CTATCTTACACTAATCTATG+TGG + chr2.1:1232592-1232611 None:intergenic 30.0%
GAAAAATAGATCCAGAAAAC+AGG - chr2.1:1232038-1232057 MS.gene85941:intron 30.0%
TAACACCTGATAACAAAGAA+TGG - chr2.1:1232239-1232258 MS.gene85941:CDS 30.0%
TAACATTAACTTTCACATGG+TGG + chr2.1:1231652-1231671 None:intergenic 30.0%
TGGCATTCAACTCAAAATAT+TGG - chr2.1:1231323-1231342 MS.gene85941:intron 30.0%
TGTATTAATTAATGCAAGGG+GGG + chr2.1:1230583-1230602 None:intergenic 30.0%
TTAACTCATGAGTTAGTATG+TGG + chr2.1:1230975-1230994 None:intergenic 30.0%
TTCATAACATGTTCGTAACA+TGG + chr2.1:1231857-1231876 None:intergenic 30.0%
TTCATTGAATCATAGATGGA+CGG + chr2.1:1230870-1230889 None:intergenic 30.0%
TTCGAGTCAATAACACAATT+TGG + chr2.1:1231000-1231019 None:intergenic 30.0%
! TGATATCAAAGTGAAAGTGT+CGG - chr2.1:1231260-1231279 MS.gene85941:intron 30.0%
! TTTCTCCATTCTTTGTTATC+AGG + chr2.1:1232247-1232266 None:intergenic 30.0%
!! AGCTTTGAATCAAAAGCAAA+TGG - chr2.1:1230378-1230397 MS.gene85941:intron 30.0%
!! AGTATTGATCTTCTCTTCTT+TGG + chr2.1:1230415-1230434 None:intergenic 30.0%
!!! ATTTTTTTTCAGTGGGCAAA+TGG - chr2.1:1232202-1232221 MS.gene85941:CDS 30.0%
!!! TTTTGAAGCTTTGAGATGAA+TGG + chr2.1:1232114-1232133 None:intergenic 30.0%
AAAGACTCTATTTCACACAC+TGG - chr2.1:1230446-1230465 MS.gene85941:intron 35.0%
AATTGTGGCTTCATGTTCTT+AGG - chr2.1:1232311-1232330 MS.gene85941:CDS 35.0%
ATAGTCATGATACGATAGGA+TGG - chr2.1:1231594-1231613 MS.gene85941:intron 35.0%
ATCATCTCTCTCAACACTTT+CGG + chr2.1:1232451-1232470 None:intergenic 35.0%
ATTGTGGCTTCATGTTCTTA+GGG - chr2.1:1232312-1232331 MS.gene85941:CDS 35.0%
CACCTTATTACCCTAAAAAG+AGG - chr2.1:1230340-1230359 MS.gene85941:intron 35.0%
GAAGTACAACAAAACAAGAG+AGG - chr2.1:1231129-1231148 MS.gene85941:intron 35.0%
GAATCAAAAGCAAATGGCAA+AGG - chr2.1:1230384-1230403 MS.gene85941:intron 35.0%
GACACTTTCACTTTGATATC+AGG + chr2.1:1231261-1231280 None:intergenic 35.0%
GAGTATGTGTATGTGTAGAT+GGG - chr2.1:1233296-1233315 MS.gene85941:CDS 35.0%
GGATAACATAAGGAAACTGT+TGG - chr2.1:1233352-1233371 MS.gene85941:CDS 35.0%
GGCTAACATTAACTTTCACA+TGG + chr2.1:1231655-1231674 None:intergenic 35.0%
GGGTTTATCTACCTCTAATA+TGG + chr2.1:1233229-1233248 None:intergenic 35.0%
GTGTTCTTTACTTGATCTGA+TGG + chr2.1:1231676-1231695 None:intergenic 35.0%
TATCCAGATCCACTGAAAAT+GGG - chr2.1:1232163-1232182 MS.gene85941:CDS 35.0%
TGAGTATGTGTATGTGTAGA+TGG - chr2.1:1233295-1233314 MS.gene85941:CDS 35.0%
TGGATAGGTTGTAGTTTATG+TGG + chr2.1:1232149-1232168 None:intergenic 35.0%
TTCATTTCACTAGATGTGGT+AGG + chr2.1:1231204-1231223 None:intergenic 35.0%
! ACAGAAGCGATGATGAATTT+TGG + chr2.1:1232079-1232098 None:intergenic 35.0%
! AGTTGGGACAAAGATTTTGA+TGG + chr2.1:1231792-1231811 None:intergenic 35.0%
! ATAAGGAAACTGTTGGTGAA+TGG - chr2.1:1233359-1233378 MS.gene85941:CDS 35.0%
! CAGAAGCGATGATGAATTTT+GGG + chr2.1:1232078-1232097 None:intergenic 35.0%
! GAATTTATCCCCATTTTCAG+TGG + chr2.1:1232175-1232194 None:intergenic 35.0%
! TTGAGGAAGTCCTCTTTTTA+GGG + chr2.1:1230353-1230372 None:intergenic 35.0%
! TTTAGGGTAATAAGGTGGTT+TGG + chr2.1:1230337-1230356 None:intergenic 35.0%
! TTTGAGGAAGTCCTCTTTTT+AGG + chr2.1:1230354-1230373 None:intergenic 35.0%
!! AACATTGACTTTCTCTTGGT+GGG - chr2.1:1232538-1232557 MS.gene85941:intron 35.0%
!! GGATTTGATTTTGCTGTGAA+GGG + chr2.1:1231821-1231840 None:intergenic 35.0%
!! TAACATTGACTTTCTCTTGG+TGG - chr2.1:1232537-1232556 MS.gene85941:intron 35.0%
!! TGGATTTGATTTTGCTGTGA+AGG + chr2.1:1231822-1231841 None:intergenic 35.0%
!!! CTCTTTTTAGGGTAATAAGG+TGG + chr2.1:1230342-1230361 None:intergenic 35.0%
!!! GTCCTCTTTTTAGGGTAATA+AGG + chr2.1:1230345-1230364 None:intergenic 35.0%
AATTCCATAGCCACAATACC+AGG - chr2.1:1230306-1230325 MS.gene85941:intron 40.0%
ATCCAGATCCACTGAAAATG+GGG - chr2.1:1232164-1232183 MS.gene85941:CDS 40.0%
ATTCCATAGCCACAATACCA+GGG - chr2.1:1230307-1230326 MS.gene85941:intron 40.0%
CAACAACCATCTATAGTAGC+CGG - chr2.1:1230210-1230229 MS.gene85941:CDS 40.0%
CCAAGAGTTGAATTGCCAAA+CGG + chr2.1:1231396-1231415 None:intergenic 40.0%
CTATCCAGATCCACTGAAAA+TGG - chr2.1:1232162-1232181 MS.gene85941:CDS 40.0%
TATAATTCCTCGCTCAACGA+AGG - chr2.1:1230521-1230540 MS.gene85941:intron 40.0%
TCATGATACGATAGGATGGT+GGG - chr2.1:1231598-1231617 MS.gene85941:intron 40.0%
TGTCAGCACTCAACTTTGTA+CGG + chr2.1:1233251-1233270 None:intergenic 40.0%
TGTGTGAAACTACCGAGATT+TGG + chr2.1:1230907-1230926 None:intergenic 40.0%
TTAGTATGTGGACGCAGTTA+CGG + chr2.1:1230963-1230982 None:intergenic 40.0%
TTTATTACCTTCGTTGAGCG+AGG + chr2.1:1230531-1230550 None:intergenic 40.0%
! CATTTTCAGTGGATCTGGAT+AGG + chr2.1:1232164-1232183 None:intergenic 40.0%
! TGGATCTAGCGGATAACATA+AGG - chr2.1:1233342-1233361 MS.gene85941:CDS 40.0%
!! AAAGTTGAGTGCTGACACAA+CGG - chr2.1:1233254-1233273 MS.gene85941:CDS 40.0%
!! AGGATGGTGGGATTTAGTAT+GGG - chr2.1:1231610-1231629 MS.gene85941:intron 40.0%
!! GAAAGTGTTGAGAGAGATGA+TGG - chr2.1:1232450-1232469 MS.gene85941:CDS 40.0%
!! GAAGCTTTGAGATGAATGGA+AGG + chr2.1:1232110-1232129 None:intergenic 40.0%
!! GGCTAACATTGACTTTCTCT+TGG - chr2.1:1232534-1232553 MS.gene85941:intron 40.0%
!! TAGGATGGTGGGATTTAGTA+TGG - chr2.1:1231609-1231628 MS.gene85941:intron 40.0%
!! TTGATTTTGCTGTGAAGGGA+AGG + chr2.1:1231817-1231836 None:intergenic 40.0%
!!! GATTTTGATGGTGTTGTTGC+TGG + chr2.1:1231780-1231799 None:intergenic 40.0%
CAAGGAATTCACCATCTTGG+TGG + chr2.1:1230176-1230195 None:intergenic 45.0%
CACCAAGATGGTGAATTCCT+TGG - chr2.1:1230174-1230193 MS.gene85941:CDS 45.0%
CCGTTTGGCAATTCAACTCT+TGG - chr2.1:1231393-1231412 MS.gene85941:intron 45.0%
GAAGAGAGAATGCTAGTGCT+AGG + chr2.1:1230282-1230301 None:intergenic 45.0%
GACAAGGCCTCTATAGTTCA+AGG - chr2.1:1231060-1231079 MS.gene85941:intron 45.0%
GATAGCGCCTTGAACTATAG+AGG + chr2.1:1231070-1231089 None:intergenic 45.0%
GGTGGGATTTAGTATGGGAA+AGG - chr2.1:1231615-1231634 MS.gene85941:intron 45.0%
GTCAGCACTCAACTTTGTAC+GGG + chr2.1:1233250-1233269 None:intergenic 45.0%
GTCATGATACGATAGGATGG+TGG - chr2.1:1231597-1231616 MS.gene85941:intron 45.0%
TATTTCACACACTGGCAGAG+AGG - chr2.1:1230454-1230473 MS.gene85941:intron 45.0%
TCAGCACTCAACTTTGTACG+GGG + chr2.1:1233249-1233268 None:intergenic 45.0%
TGAATTCCTTGGCGACTACA+TGG - chr2.1:1230185-1230204 MS.gene85941:CDS 45.0%
TGCATACAGGATGACGATGA+TGG - chr2.1:1233322-1233341 MS.gene85941:CDS 45.0%
TGGCATTACTATCATCGCTC+AGG - chr2.1:1231413-1231432 MS.gene85941:intron 45.0%
TGTAGATGGGTGATGCATAC+AGG - chr2.1:1233309-1233328 MS.gene85941:CDS 45.0%
TTGGTATGAAGGAGTGATGG+TGG + chr2.1:1231748-1231767 None:intergenic 45.0%
TTTCAGTGGGCAAATGGTGA+AGG - chr2.1:1232208-1232227 MS.gene85941:CDS 45.0%
TTTGGACTTCCCTGGTATTG+TGG + chr2.1:1230319-1230338 None:intergenic 45.0%
! AGTGTTGAGAGAGATGATGG+TGG - chr2.1:1232453-1232472 MS.gene85941:CDS 45.0%
! ATCCCCATTTTCAGTGGATC+TGG + chr2.1:1232169-1232188 None:intergenic 45.0%
! TCAAGGCGCTATCAATTACG+TGG - chr2.1:1231077-1231096 MS.gene85941:intron 45.0%
! TCCTTTTCGACAGACAGACA+AGG - chr2.1:1231044-1231063 MS.gene85941:intron 45.0%
! TGGTTGGTATGAAGGAGTGA+TGG + chr2.1:1231751-1231770 None:intergenic 45.0%
!! AGAGAGATGATGGTGGAAGA+TGG - chr2.1:1232460-1232479 MS.gene85941:CDS 45.0%
!! AGTGATGGTGGTGGAAGATA+GGG + chr2.1:1231736-1231755 None:intergenic 45.0%
!! GATGACGATGATGGATCTAG+CGG - chr2.1:1233331-1233350 MS.gene85941:CDS 45.0%
!! GCATCTAGCTTCCTGTTTTC+TGG + chr2.1:1232052-1232071 None:intergenic 45.0%
!! TTGATGGTGTTGTTGCTGGT+GGG + chr2.1:1231776-1231795 None:intergenic 45.0%
!! TTTGATGGTGTTGTTGCTGG+TGG + chr2.1:1231777-1231796 None:intergenic 45.0%
!! AATATGAATATATAATTTAT+AGG - chr2.1:1233022-1233041 MS.gene85941:CDS 5.0%
!! ATTAAAATAAAATAAAATTG+TGG - chr2.1:1232296-1232315 MS.gene85941:CDS 5.0%
!!! ATTTTTCAATAAAAATAAAA+AGG + chr2.1:1232025-1232044 None:intergenic 5.0%
ATTGCCAAACGGCAATACGC+TGG + chr2.1:1231385-1231404 None:intergenic 50.0%
CGCCAAGGAATTCACCATCT+TGG + chr2.1:1230179-1230198 None:intergenic 50.0%
CTGTGAAGGGAAGGAGAGTT+GGG + chr2.1:1231808-1231827 None:intergenic 50.0%
CTTCCCTGGTATTGTGGCTA+TGG + chr2.1:1230313-1230332 None:intergenic 50.0%
GAATCGATGTGCCACCAAGA+TGG - chr2.1:1230162-1230181 MS.gene85941:CDS 50.0%
GCCTTGTCTGTCTGTCGAAA+AGG + chr2.1:1231048-1231067 None:intergenic 50.0%
! TAAGGTGGTTTGGACTTCCC+TGG + chr2.1:1230327-1230346 None:intergenic 50.0%
!! GAGTGATGGTGGTGGAAGAT+AGG + chr2.1:1231737-1231756 None:intergenic 50.0%
!! GTATGAAGGAGTGATGGTGG+TGG + chr2.1:1231745-1231764 None:intergenic 50.0%
!! TGATGGTGTTGTTGCTGGTG+GGG + chr2.1:1231775-1231794 None:intergenic 50.0%
!! TTGAGTGCTGACACAACGGT+AGG - chr2.1:1233258-1233277 MS.gene85941:CDS 50.0%
AAACGGCAATACGCTGGTGC+TGG + chr2.1:1231379-1231398 None:intergenic 55.0%
ACACACTGGCAGAGAGGAGA+AGG - chr2.1:1230460-1230479 MS.gene85941:intron 55.0%
AGCACCAGCGTATTGCCGTT+TGG - chr2.1:1231378-1231397 MS.gene85941:intron 55.0%
CACGAAGATTGCAAGCTCGC+CGG - chr2.1:1230248-1230267 MS.gene85941:intron 55.0%
CTAGTGCTAGGAAGCAGCTC+CGG + chr2.1:1230270-1230289 None:intergenic 55.0%
GCTGTGAAGGGAAGGAGAGT+TGG + chr2.1:1231809-1231828 None:intergenic 55.0%
GCTTGCAATCTTCGTGGCAG+CGG + chr2.1:1230244-1230263 None:intergenic 55.0%
GTTGTGCCATGTAGTCGCCA+AGG + chr2.1:1230194-1230213 None:intergenic 55.0%
! TTGTTGCTGGTGGGGTTGGT+TGG + chr2.1:1231767-1231786 None:intergenic 55.0%
!! GGTGGGGTTGGTTGGTATGA+AGG + chr2.1:1231759-1231778 None:intergenic 55.0%
AATCTTCGTGGCAGCGGTGG+CGG + chr2.1:1230238-1230257 None:intergenic 60.0%
CGGCGAGCTTGCAATCTTCG+TGG + chr2.1:1230250-1230269 None:intergenic 60.0%
GCGGCTCCGGCTACTATAGA+TGG + chr2.1:1230219-1230238 None:intergenic 60.0%
TGCAATCTTCGTGGCAGCGG+TGG + chr2.1:1230241-1230260 None:intergenic 60.0%
!! GGTGTTGTTGCTGGTGGGGT+TGG + chr2.1:1231771-1231790 None:intergenic 60.0%
!! CGTGGCAGCGGTGGCGGCTC+CGG + chr2.1:1230232-1230251 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 1230150 1233400 1230150 ID=MS.gene85941
chr2.1 mRNA 1230150 1233400 1230150 ID=MS.gene85941.t1;Parent=MS.gene85941
chr2.1 exon 1233008 1233400 1233008 ID=MS.gene85941.t1.exon1;Parent=MS.gene85941.t1
chr2.1 CDS 1233008 1233400 1233008 ID=cds.MS.gene85941.t1;Parent=MS.gene85941.t1
chr2.1 exon 1232116 1232493 1232116 ID=MS.gene85941.t1.exon2;Parent=MS.gene85941.t1
chr2.1 CDS 1232116 1232493 1232116 ID=cds.MS.gene85941.t1;Parent=MS.gene85941.t1
chr2.1 exon 1230150 1230236 1230150 ID=MS.gene85941.t1.exon3;Parent=MS.gene85941.t1
chr2.1 CDS 1230150 1230236 1230150 ID=cds.MS.gene85941.t1;Parent=MS.gene85941.t1
Gene Sequence

>MS.gene85941

ATGGATTGGCTAGAATCGATGTGCCACCAAGATGGTGAATTCCTTGGCGACTACATGGCACAACAACCATCTATAGTAGCCGGAGCCGCCACCGCTGCCACGAAGATTGCAAGCTCGCCGGAGCTGCTTCCTAGCACTAGCATTCTCTCTTCTAATAATTCCATAGCCACAATACCAGGGAAGTCCAAACCACCTTATTACCCTAAAAAGAGGACTTCCTCAAATATAAGCTTTGAATCAAAAGCAAATGGCAAAGGATTAGCCAAAGAAGAGAAGATCAATACTAGAAGCAAATCAAAGACTCTATTTCACACACTGGCAGAGAGGAGAAGGAGACTTGAACTCGCACATAAATTCACAGAGCTTTCATCTATAATTCCTCGCTCAACGAAGGTAATAAACTCAACAAATTATTTAGACTCAATTTTTACCCCCCTTGCATTAATTAATACATTTCAGTGCTTTGATTTGAATTACATACATACTGAATCAAATCAAAACACATGATGTAATAAAACAAATCAAAACAAAACAAATGATGTAATAAAACAAATCAAAACAAAGCATTCATCATAGTTTTTTTATATTATTTATTTTGAATATAAATATACTCCTATATTAACTTTTTAGGGAAAAAAAGAGAATTTTTTGGAAAATATGTTAAAAACTTATAGTGAACGAATTCACAAAATTCAAAGTAGTAGTAATAAATCTCGACCGTCCATCTATGATTCAATGAATCTTAATACACAATCCAAATCTCGGTAGTTTCACACAATGTGAAATCTCAATCGTTCTTTTACAATCAGACCGTAACTGCGTCCACATACTAACTCATGAGTTAAATCCAAATTGTGTTATTGACTCGAATTTAATGGTTTCTGCATATGTATTTCCTTTTCGACAGACAGACAAGGCCTCTATAGTTCAAGGCGCTATCAATTACGTGGAAAAACTTCAAAAAAGAGTGATGGAGTTAGAAGTACAACAAAACAAGAGAGGCAAAGAACCAATAATACTTCACAATAAGGAAAATTATTGTGGAGTTCGACCTACCACATCTAGTGAAATGAATCTTGACAATTATCTTAGACCAAATGAATTGCTTCCTGATATCAAAGTGAAAGTGTCGGAGAATAATATACTCATTTACATCAATTGTGAGAAGGAAAATGGCATTCAACTCAAAATATTGGACATGCTTCAAAATCTTCATTTGTTTGTCACCAGCACCAGCGTATTGCCGTTTGGCAATTCAACTCTTGGCATTACTATCATCGCTCAGGTATGCATCTCTAAACTTTAATGCCCTACTTTAACACTACATCAAAACTAATTATGAACATTTTCTTTGACAAATTGTACTAATTATAAACATGCATGAGAAATGTAGTTTTTGCTATAACTTTTATAAAAATAAAAAATAAAATAAAACATTTGTTTAATAGTCATGATACGATAGGATGGTGGGATTTAGTATGGGAAAGGAGAGTTGTTTCCCACCATGTGAAAGTTAATGTTAGCCATCAGATCAAGTAAAGAACACAATCTTATCATACACTCTCAACACTAGATTATTTTGTCCCTATCTTCCACCACCATCACTCCTTCATACCAACCAACCCCACCAGCAACAACACCATCAAAATCTTTGTCCCAACTCTCCTTCCCTTCACAGCAAAATCAAATCCAAATCAAGTAAAACCATGTTACGAACATGTTATGAACAACCATTTTTCTTTGATTTTTTTTCTTCCTAATGAAATACCAAACTCTTTTCTTCAATTCCTTAATCATCTTCTTATTCTTCACCCACTCTCTTTATAACAAAATACCAATTTTGTGAAATTACTTTCTTTCTCTTTGTTTTATCCTTTTTATTTTTATTGAAAAATAGATCCAGAAAACAGGAAGCTAGATGCAGTCCCAAAATTCATCATCGCTTCTGTCTTTAACTCCTTCCATTCATCTCAAAGCTTCAAAATTTCAAACACAACCACATAAACTACAACCTATCCAGATCCACTGAAAATGGGGATAAATTCTTTGAGAATTTTTTTTCAGTGGGCAAATGGTGAAGGAAATAAAATAACACCTGATAACAAAGAATGGAGAAAAAAATTCAATAATTGAACAAATCATAGAAATTAAAATAAAATAAAATTGTGGCTTCATGTTCTTAGGGCAACACATGATGTGAAATTGAAAGAAGAAAAAAAAACTGCGAATTCATGCTGTGATACCGTCAAGCACCCATTTTATTTTAATTTCATGTTCTGGGTTATTAAGTTTTGATTTCCGAAAGTGTTGAGAGAGATGATGGTGGAAGATGGTGACAAAAAAATTCTAGTGTTGAAGGTGTATAATAAGATTGTGTATCTAATGGCTAACATTGACTTTCTCTTGGTGGGAAACAACTATGCTTTCCCATGCTAAATTCCACATAGATTAGTGTAAGATAGAATATAAAAATAGATATAATATGATATATATATATATATATATATATATATATATATATATATATATATATATATATATAGACATGGTAAGTCTAATCGTATCTTATGTTTGATGTACAATAAAAATAAACTTAAATATTATATATTATTTTATTTGTAGATTTTAGATAAACTAAGTATTGATATGAGATACATTATTATATGTAAATGAAAAAGCTATCATTGTGGAAAAGTTATATTGAATTTTTTTAAAATGAAACGAAATTTCTTAATTTTAATAATAATAGTAATAATATAATAAATTTACTAAAAAATAATAATAATAATAATAATTTTATTTTAAGAATTAAATATTTTTATTCCAATAGAATTTAAAAGAGCCAAAATTCATTGTGATAAAATTATATATAAATATGAATATATAATTTATAGGAATATAATTTTAATATAATAAATATTATTATATTTTTAAAAAAGTTATATTTAAATTATACTTTATAAAAGTAAATGATAATTTTTTTTATCATATCTAACTGGATTCTAGAAAAATAAAAAATCATTGGTAACATAATTTTCTTCTAAGAAAAAAAAAAGAATTTCATTCATAAAGTCATCCATATTAGAGGTAGATAAACCCCGTACAAAGTTGAGTGCTGACACAACGGTAGGCATGTCTCTGATTGTGAGTATGTGTATGTGTAGATGGGTGATGCATACAGGATGACGATGATGGATCTAGCGGATAACATAAGGAAACTGTTGGTGAATGGCGCAAATCGTCGAACATGA
Protein sequence

>MS.gene85941.t1

MDWLESMCHQDGEFLGDYMAQQPSIVAGAATAATKIASSPELLPSTSILSSNNSIATIPGKSKPPYYPKKRTSSNISFESKANGKGLAKEEKINTRSKSKTLFHTLAERRRRLELAHKFTELSSIIPRSTKTDKASIVQGAINYVEKLQKRVMELEVQQNKRGKEPIILHNKENYCGVRPTTSSEMNLDNYLRPNELLPDIKVKVSENNILIYINCEKENGIQLKILDMLQNLHLFVTSTSVLPFGNSTLGITIIAQMGDAYRMTMMDLADNIRKLLVNGANRRT