Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86016.t1 | XP_013444664.1 | 95.6 | 90 | 4 | 0 | 1 | 90 | 70 | 159 | 8.50E-41 | 176 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86016.t1 | Q8H0Y8 | 56.3 | 71 | 25 | 2 | 24 | 90 | 96 | 164 | 4.2e-12 | 72.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86016.t1 | A0A072TMF1 | 95.6 | 90 | 4 | 0 | 1 | 90 | 70 | 159 | 6.1e-41 | 176.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene86016.t1 | TF | WRKY |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86016.t1 | MTR_8g027860 | 95.556 | 90 | 4 | 0 | 1 | 90 | 70 | 159 | 3.40e-58 | 182 |
| MS.gene86016.t1 | MTR_7g117200 | 62.025 | 79 | 26 | 3 | 16 | 90 | 63 | 141 | 6.00e-23 | 90.5 |
| MS.gene86016.t1 | MTR_8g099350 | 57.534 | 73 | 27 | 2 | 22 | 90 | 83 | 155 | 8.97e-20 | 82.0 |
| MS.gene86016.t1 | MTR_3g090860 | 45.652 | 92 | 44 | 3 | 14 | 99 | 78 | 169 | 3.33e-18 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86016.t1 | AT4G11070 | 56.338 | 71 | 25 | 2 | 24 | 90 | 64 | 132 | 9.64e-18 | 75.9 |
| MS.gene86016.t1 | AT4G11070 | 56.338 | 71 | 25 | 2 | 24 | 90 | 96 | 164 | 1.04e-17 | 76.3 |
| MS.gene86016.t1 | AT4G23810 | 50.649 | 77 | 29 | 2 | 23 | 90 | 105 | 181 | 2.47e-17 | 75.1 |
| MS.gene86016.t1 | AT5G24110 | 47.761 | 67 | 33 | 1 | 24 | 90 | 72 | 136 | 3.34e-16 | 72.0 |
| MS.gene86016.t1 | AT2G46400 | 59.259 | 54 | 21 | 1 | 38 | 90 | 74 | 127 | 4.43e-13 | 63.2 |
Find 23 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGCAAGTGAAGGTTTATTC+TGG | 0.340277 | 8.2:-69560001 | MS.gene86016:CDS |
| TGTGCTTGAACTTTGAATCT+TGG | 0.345830 | 8.2:+69560207 | None:intergenic |
| GGTTATAGTTGGAGAAAGTA+TGG | 0.368943 | 8.2:-69559950 | MS.gene86016:CDS |
| TATGGACAAAAGGATATTCT+TGG | 0.372931 | 8.2:-69559932 | MS.gene86016:CDS |
| ATGGATTCTCATTGTTCAAA+TGG | 0.390219 | 8.2:-69560248 | MS.gene86016:CDS |
| CTGTTGAGGATGGTTATAGT+TGG | 0.443101 | 8.2:-69559961 | MS.gene86016:CDS |
| TTGGAGAAAGTATGGACAAA+AGG | 0.456392 | 8.2:-69559942 | MS.gene86016:CDS |
| TCCCTCATTACCCTTTGCCA+TGG | 0.480628 | 8.2:+69560298 | None:intergenic |
| AACCATGGCAAAGGGTAATG+AGG | 0.498135 | 8.2:-69560300 | None:intergenic |
| ACAAACATGTGTCCAAGAAA+AGG | 0.507877 | 8.2:-69560187 | MS.gene86016:intron |
| GACAAACAATGTCAACATGA+TGG | 0.527140 | 8.2:-69560267 | MS.gene86016:CDS |
| TTCTTGGAGCTAAGTTTCCA+AGG | 0.535081 | 8.2:-69559916 | MS.gene86016:intron |
| TTCACTTGCTCTGTCCACTT+AGG | 0.560248 | 8.2:+69560013 | None:intergenic |
| GACAGCAATAACCATGGCAA+AGG | 0.573478 | 8.2:-69560309 | None:intergenic |
| ACAGCAATAACCATGGCAAA+GGG | 0.573968 | 8.2:-69560308 | None:intergenic |
| TATTCTGGAACAGAAGCTGA+AGG | 0.581805 | 8.2:-69559986 | MS.gene86016:CDS |
| ACCATGGCAAAGGGTAATGA+GGG | 0.583479 | 8.2:-69560299 | MS.gene86016:CDS |
| GCTGAAGGGTCTGTTGAGGA+TGG | 0.595548 | 8.2:-69559971 | MS.gene86016:CDS |
| TTGCTCTGTCCACTTAGGCA+CGG | 0.602998 | 8.2:+69560018 | None:intergenic |
| ATTCTGGAACAGAAGCTGAA+GGG | 0.629611 | 8.2:-69559985 | MS.gene86016:CDS |
| AAATGGAAGCCCTAAAAGTG+AGG | 0.654127 | 8.2:-69560231 | MS.gene86016:CDS |
| AGAAGCTGAAGGGTCTGTTG+AGG | 0.659448 | 8.2:-69559975 | MS.gene86016:CDS |
| TAAGTGGACAGAGCAAGTGA+AGG | 0.673359 | 8.2:-69560011 | MS.gene86016:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATACTATATCAAATAA+TGG | - | chr8.2:69559871-69559890 | MS.gene86016:intron | 10.0% |
| !! | AAAATACTATATCAAATAAT+GGG | - | chr8.2:69559872-69559891 | MS.gene86016:intron | 10.0% |
| !!! | TAGGTTTTTATTTATTATAT+AGG | - | chr8.2:69559748-69559767 | MS.gene86016:intron | 10.0% |
| !! | AAAAAAAAAAAATTGTCCTT+TGG | + | chr8.2:69559936-69559955 | None:intergenic | 15.0% |
| !! | AAATTAGAAAGATTTAATTC+TGG | - | chr8.2:69559976-69559995 | MS.gene86016:CDS | 15.0% |
| !! | TATTTAATGCTAACTTAAAT+TGG | + | chr8.2:69560082-69560101 | None:intergenic | 15.0% |
| !!! | ATAATTTTGTCAAATTTACT+AGG | + | chr8.2:69559489-69559508 | None:intergenic | 15.0% |
| !!! | ATATAGGAATTTTTTTTGAT+TGG | - | chr8.2:69559764-69559783 | MS.gene86016:intron | 15.0% |
| !!! | TCATAATTTTCATTATTCTT+TGG | - | chr8.2:69559251-69559270 | MS.gene86016:intron | 15.0% |
| !!! | TTTTTTTTTTAAGAATCCAA+AGG | - | chr8.2:69559950-69559969 | MS.gene86016:CDS | 15.0% |
| !! | AAGTTGTATTTGTTCTATTT+AGG | - | chr8.2:69559729-69559748 | MS.gene86016:intron | 20.0% |
| !! | CATTGGAAAAATTTAACAAT+TGG | - | chr8.2:69560022-69560041 | MS.gene86016:CDS | 20.0% |
| !!! | AAGAGTTTTGTTATTCATTA+TGG | - | chr8.2:69559512-69559531 | MS.gene86016:intron | 20.0% |
| !!! | AATCTGACAATTTTAACTAA+TGG | + | chr8.2:69560202-69560221 | None:intergenic | 20.0% |
| !!! | AATTTAATTACTTTTGCTTG+AGG | - | chr8.2:69559605-69559624 | MS.gene86016:intron | 20.0% |
| !!! | TATAGGAATTTTTTTTTCCA+AGG | - | chr8.2:69559219-69559238 | MS.gene86016:intron | 20.0% |
| !!! | TTCAAGGTTCATTTTTTTAT+AGG | - | chr8.2:69559202-69559221 | MS.gene86016:intron | 20.0% |
| ! | ACCAATAATGTCTAAAAAAG+AGG | - | chr8.2:69559836-69559855 | MS.gene86016:intron | 25.0% |
| ! | AGGAATTTAAAGATGCATTA+TGG | + | chr8.2:69559469-69559488 | None:intergenic | 25.0% |
| ! | CCAATAATGTCTAAAAAAGA+GGG | - | chr8.2:69559837-69559856 | MS.gene86016:intron | 25.0% |
| ! | CTAACCTAAAGTCAAAATAT+AGG | - | chr8.2:69560257-69560276 | MS.gene86016:CDS | 25.0% |
| ! | GTAAAAAATGATTGTTACCT+TGG | + | chr8.2:69559416-69559435 | None:intergenic | 25.0% |
| ! | TAGAAAGATTTAATTCTGGT+AGG | - | chr8.2:69559980-69559999 | MS.gene86016:CDS | 25.0% |
| ! | TATATATTAATCTCCTGAGT+CGG | + | chr8.2:69559544-69559563 | None:intergenic | 25.0% |
| ! | TGGAACCATTAATACAATTA+TGG | - | chr8.2:69560174-69560193 | MS.gene86016:intron | 25.0% |
| ! | TGTATTAATGGTTCCATAAT+TGG | + | chr8.2:69560170-69560189 | None:intergenic | 25.0% |
| !! | AAAAAAAGTTATTTGCTGCA+TGG | + | chr8.2:69559644-69559663 | None:intergenic | 25.0% |
| !! | AAAAAAGTTATTTGCTGCAT+GGG | + | chr8.2:69559643-69559662 | None:intergenic | 25.0% |
| !! | ACTATATCAAATAATGGGAA+AGG | - | chr8.2:69559877-69559896 | MS.gene86016:intron | 25.0% |
| !! | ATCTTTCTAATTTTGTCCTT+TGG | + | chr8.2:69559969-69559988 | None:intergenic | 25.0% |
| !! | TAATCCAATATACCTTTTCT+TGG | + | chr8.2:69559140-69559159 | None:intergenic | 25.0% |
| !! | TTTAGACATTATTGGTTGAT+TGG | + | chr8.2:69559832-69559851 | None:intergenic | 25.0% |
| !!! | ATGACCTATATTTTGACTTT+AGG | + | chr8.2:69560264-69560283 | None:intergenic | 25.0% |
| !!! | CATTATTCTTTGGAATTTTG+TGG | - | chr8.2:69559261-69559280 | MS.gene86016:intron | 25.0% |
| AACTTAAATTGGTGCATGAA+TGG | + | chr8.2:69560071-69560090 | None:intergenic | 30.0% | |
| AGACGATAAAAGTTATCCAA+AGG | - | chr8.2:69559917-69559936 | MS.gene86016:CDS | 30.0% | |
| ATAATGTCTAAAAAAGAGGG+AGG | - | chr8.2:69559840-69559859 | MS.gene86016:intron | 30.0% | |
| ATGGATTCTCATTGTTCAAA+TGG | - | chr8.2:69559064-69559083 | MS.gene86016:intron | 30.0% | |
| GTTGTCTATAACAGTGAATT+TGG | + | chr8.2:69560127-69560146 | None:intergenic | 30.0% | |
| TAATGTCTAAAAAAGAGGGA+GGG | - | chr8.2:69559841-69559860 | MS.gene86016:intron | 30.0% | |
| TATGGACAAAAGGATATTCT+TGG | - | chr8.2:69559380-69559399 | MS.gene86016:intron | 30.0% | |
| TGGCTCCATAATTGTATTAA+TGG | + | chr8.2:69560182-69560201 | None:intergenic | 30.0% | |
| ! | AAAAAGTTATTTGCTGCATG+GGG | + | chr8.2:69559642-69559661 | None:intergenic | 30.0% |
| !!! | CAATGATATTTTAGTGAGTG+GGG | + | chr8.2:69559808-69559827 | None:intergenic | 30.0% |
| !!! | CCAATGATATTTTAGTGAGT+GGG | + | chr8.2:69559809-69559828 | None:intergenic | 30.0% |
| !!! | CCCTCTTTTTTAGACATTAT+TGG | + | chr8.2:69559840-69559859 | None:intergenic | 30.0% |
| !!! | TCATTGATTCCTTTTGAACT+TGG | - | chr8.2:69559170-69559189 | MS.gene86016:intron | 30.0% |
| !!! | TTTTTTTTGATTGGTGCAAG+TGG | - | chr8.2:69559773-69559792 | MS.gene86016:intron | 30.0% |
| AATTATGAGATCGTGAACCT+TGG | + | chr8.2:69559239-69559258 | None:intergenic | 35.0% | |
| AATTCACCACCAAGTTCAAA+AGG | + | chr8.2:69559182-69559201 | None:intergenic | 35.0% | |
| ACAAACATGTGTCCAAGAAA+AGG | - | chr8.2:69559125-69559144 | MS.gene86016:intron | 35.0% | |
| ATTATGGTATTATCCGACTC+AGG | - | chr8.2:69559528-69559547 | MS.gene86016:intron | 35.0% | |
| CATGTGTACTACACCAATTA+TGG | - | chr8.2:69560154-69560173 | MS.gene86016:intron | 35.0% | |
| CCCACTCACTAAAATATCAT+TGG | - | chr8.2:69559806-69559825 | MS.gene86016:intron | 35.0% | |
| GACAAACAATGTCAACATGA+TGG | - | chr8.2:69559045-69559064 | MS.gene86016:intron | 35.0% | |
| GGTTATAGTTGGAGAAAGTA+TGG | - | chr8.2:69559362-69559381 | MS.gene86016:intron | 35.0% | |
| TACTAGAAGAGAAAGGACAT+TGG | - | chr8.2:69560005-69560024 | MS.gene86016:CDS | 35.0% | |
| TGTGCTTGAACTTTGAATCT+TGG | + | chr8.2:69559108-69559127 | None:intergenic | 35.0% | |
| ! | GTGTCCAAGAAAAGGTATAT+TGG | - | chr8.2:69559133-69559152 | MS.gene86016:intron | 35.0% |
| ! | TTGATTCCTTTTGAACTTGG+TGG | - | chr8.2:69559173-69559192 | MS.gene86016:intron | 35.0% |
| ! | TTGGAGAAAGTATGGACAAA+AGG | - | chr8.2:69559370-69559389 | MS.gene86016:intron | 35.0% |
| !! | AATCTTGGACCTCACTTTTA+GGG | + | chr8.2:69559093-69559112 | None:intergenic | 35.0% |
| !!! | AACTTGGTGGTGAATTTTCA+AGG | - | chr8.2:69559186-69559205 | MS.gene86016:intron | 35.0% |
| !!! | GCCAATGATATTTTAGTGAG+TGG | + | chr8.2:69559810-69559829 | None:intergenic | 35.0% |
| AAATGGAAGCCCTAAAAGTG+AGG | - | chr8.2:69559081-69559100 | MS.gene86016:intron | 40.0% | |
| CTGTTGAGGATGGTTATAGT+TGG | - | chr8.2:69559351-69559370 | MS.gene86016:intron | 40.0% | |
| GAGCAAGTGAAGGTTTATTC+TGG | - | chr8.2:69559311-69559330 | MS.gene86016:intron | 40.0% | |
| GTAGGTGTACTAGAAGAGAA+AGG | - | chr8.2:69559998-69560017 | MS.gene86016:CDS | 40.0% | |
| TTCTTGGAGCTAAGTTTCCA+AGG | - | chr8.2:69559396-69559415 | MS.gene86016:intron | 40.0% | |
| !! | ATTCTGGAACAGAAGCTGAA+GGG | - | chr8.2:69559327-69559346 | MS.gene86016:intron | 40.0% |
| !! | GAATCTTGGACCTCACTTTT+AGG | + | chr8.2:69559094-69559113 | None:intergenic | 40.0% |
| !! | TATTCTGGAACAGAAGCTGA+AGG | - | chr8.2:69559326-69559345 | MS.gene86016:intron | 40.0% |
| TAAGTGGACAGAGCAAGTGA+AGG | - | chr8.2:69559301-69559320 | MS.gene86016:intron | 45.0% | |
| TGATTGGTGCAAGTGGCTTT+GGG | - | chr8.2:69559780-69559799 | MS.gene86016:intron | 45.0% | |
| TTCACTTGCTCTGTCCACTT+AGG | + | chr8.2:69559302-69559321 | None:intergenic | 45.0% | |
| TTGATTGGTGCAAGTGGCTT+TGG | - | chr8.2:69559779-69559798 | MS.gene86016:intron | 45.0% | |
| AGAAGCTGAAGGGTCTGTTG+AGG | - | chr8.2:69559337-69559356 | MS.gene86016:intron | 50.0% | |
| GCAGAAAAACCGTGCCTAAG+TGG | - | chr8.2:69559285-69559304 | MS.gene86016:intron | 50.0% | |
| TTGCTCTGTCCACTTAGGCA+CGG | + | chr8.2:69559297-69559316 | None:intergenic | 50.0% | |
| GCTGAAGGGTCTGTTGAGGA+TGG | - | chr8.2:69559341-69559360 | MS.gene86016:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 69559016 | 69560318 | 69559016 | ID=MS.gene86016 |
| chr8.2 | mRNA | 69559016 | 69560318 | 69559016 | ID=MS.gene86016.t1;Parent=MS.gene86016 |
| chr8.2 | exon | 69560188 | 69560318 | 69560188 | ID=MS.gene86016.t1.exon1;Parent=MS.gene86016.t1 |
| chr8.2 | CDS | 69560188 | 69560318 | 69560188 | ID=cds.MS.gene86016.t1;Parent=MS.gene86016.t1 |
| chr8.2 | exon | 69559917 | 69560045 | 69559917 | ID=MS.gene86016.t1.exon2;Parent=MS.gene86016.t1 |
| chr8.2 | CDS | 69559917 | 69560045 | 69559917 | ID=cds.MS.gene86016.t1;Parent=MS.gene86016.t1 |
| chr8.2 | exon | 69559016 | 69559055 | 69559016 | ID=MS.gene86016.t1.exon3;Parent=MS.gene86016.t1 |
| chr8.2 | CDS | 69559016 | 69559055 | 69559016 | ID=cds.MS.gene86016.t1;Parent=MS.gene86016.t1 |
| Gene Sequence |
| Protein sequence |
