AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86226

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86226.t1 MTR_7g446520 47.710 262 90 5 13 258 32 262 1.05e-63 217
MS.gene86226.t1 MTR_7g028855 52.000 200 91 4 14 209 65 263 9.71e-55 196
MS.gene86226.t1 MTR_7g446480 62.069 116 44 0 143 258 140 255 1.42e-42 158
MS.gene86226.t1 MTR_7g446510 61.207 116 45 0 143 258 155 270 8.05e-41 153
MS.gene86226.t1 MTR_1g070355 34.201 269 159 6 1 261 9 267 9.56e-37 146
MS.gene86226.t1 MTR_5g076070 32.083 240 156 3 38 277 26 258 1.33e-34 134
MS.gene86226.t1 MTR_6g022080 31.250 176 118 2 55 228 73 247 4.91e-25 105
MS.gene86226.t1 MTR_0402s0040 31.111 180 113 3 67 242 28 200 1.79e-20 96.3
MS.gene86226.t1 MTR_6g051425 28.796 191 133 3 73 261 25 214 9.62e-20 92.0
MS.gene86226.t1 MTR_8g468460 50.526 95 46 1 275 369 12 105 9.92e-20 85.5
MS.gene86226.t1 MTR_2g098880 31.677 161 100 5 13 169 10 164 2.51e-15 77.0
MS.gene86226.t1 MTR_2g098880 31.677 161 100 5 13 169 22 176 3.65e-15 77.0
MS.gene86226.t1 MTR_0645s0010 38.000 100 57 1 261 360 31 125 6.37e-15 72.4
MS.gene86226.t1 MTR_4g035820 29.130 230 138 8 71 293 81 292 2.24e-14 77.4
MS.gene86226.t1 MTR_6g053140 31.373 153 87 5 16 158 44 188 9.16e-14 71.6
MS.gene86226.t1 MTR_1g008290 33.333 114 71 2 134 247 18 126 3.73e-13 68.2
MS.gene86226.t1 MTR_2g098920 36.538 104 62 2 70 173 85 184 6.88e-13 70.1
MS.gene86226.t1 MTR_2g101460 36.066 122 77 1 56 177 46 166 2.19e-12 67.4
MS.gene86226.t1 MTR_3g450850 28.409 176 114 5 71 236 53 226 3.22e-12 69.3
MS.gene86226.t1 MTR_2g101460 36.066 122 77 1 56 177 36 156 3.41e-12 66.6
MS.gene86226.t1 MTR_8g467420 39.024 82 48 2 50 130 63 143 2.01e-11 63.5
MS.gene86226.t1 MTR_5g015480 28.571 126 82 2 67 189 58 178 2.41e-11 65.5
MS.gene86226.t1 MTR_4g067290 32.812 128 81 3 70 196 81 204 8.14e-11 63.9
MS.gene86226.t1 MTR_4g067290 32.812 128 81 3 70 196 61 184 8.28e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86226.t1 AT4G38180 30.566 265 161 7 16 261 8 268 5.44e-22 102
MS.gene86226.t1 AT4G38180 23.478 230 174 2 267 496 492 719 3.01e-14 77.4
MS.gene86226.t1 AT1G10240 33.520 179 110 5 71 243 54 229 4.46e-18 89.7
MS.gene86226.t1 AT1G10240 33.520 179 110 5 71 243 54 229 4.46e-18 89.7
MS.gene86226.t1 AT4G15090 28.627 255 164 9 262 508 463 707 2.22e-16 84.3
MS.gene86226.t1 AT4G15090 27.803 223 148 6 69 290 55 265 2.19e-14 77.8
MS.gene86226.t1 AT4G15090 28.627 255 164 9 262 508 463 707 2.22e-16 84.3
MS.gene86226.t1 AT4G15090 27.803 223 148 6 69 290 55 265 2.19e-14 77.8
MS.gene86226.t1 AT4G15090 28.627 255 164 9 262 508 463 707 2.22e-16 84.3
MS.gene86226.t1 AT4G15090 27.803 223 148 6 69 290 55 265 2.19e-14 77.8
MS.gene86226.t1 AT4G15090 28.627 255 164 9 262 508 463 707 2.22e-16 84.3
MS.gene86226.t1 AT4G15090 27.803 223 148 6 69 290 55 265 2.19e-14 77.8
MS.gene86226.t1 AT4G15090 28.627 255 164 9 262 508 463 707 2.22e-16 84.3
MS.gene86226.t1 AT4G15090 27.803 223 148 6 69 290 55 265 2.19e-14 77.8
MS.gene86226.t1 AT3G22170 28.191 188 126 4 262 449 472 650 3.62e-14 77.0
MS.gene86226.t1 AT3G22170 28.191 188 126 4 262 449 472 650 3.62e-14 77.0
MS.gene86226.t1 AT4G38170 28.472 144 102 1 268 411 258 400 9.16e-14 75.5
MS.gene86226.t1 AT5G18960 28.571 161 111 2 254 414 576 732 9.67e-14 75.9
MS.gene86226.t1 AT5G18960 28.571 161 111 2 254 414 576 732 9.67e-14 75.9
MS.gene86226.t1 AT3G06250 29.139 151 106 1 264 414 559 708 1.49e-13 75.1
MS.gene86226.t1 AT3G06250 29.139 151 106 1 264 414 559 708 1.49e-13 75.1
MS.gene86226.t1 AT3G06250 29.139 151 106 1 264 414 559 708 1.49e-13 75.1
MS.gene86226.t1 AT2G27110 28.571 161 112 2 268 426 293 452 4.58e-13 73.6
MS.gene86226.t1 AT3G59470 30.675 163 108 3 12 173 16 174 5.32e-13 70.1
MS.gene86226.t1 AT3G59470 30.675 163 108 3 12 173 16 174 5.32e-13 70.1
MS.gene86226.t1 AT2G27110 28.571 161 112 2 268 426 438 597 5.76e-13 73.2
MS.gene86226.t1 AT2G27110 28.571 161 112 2 268 426 438 597 5.76e-13 73.2
MS.gene86226.t1 AT3G59470 30.675 163 108 3 12 173 28 186 6.53e-13 70.1
MS.gene86226.t1 AT5G28530 27.439 164 110 5 71 229 61 220 3.72e-12 70.5
MS.gene86226.t1 AT5G28530 27.439 164 110 5 71 229 61 220 4.36e-12 70.1
MS.gene86226.t1 AT5G28530 27.439 164 110 5 71 229 61 220 5.66e-12 70.1
MS.gene86226.t1 AT5G28530 27.439 164 110 5 71 229 61 220 6.47e-12 69.7
MS.gene86226.t1 AT5G28530 27.439 164 110 5 71 229 61 220 6.47e-12 69.7
MS.gene86226.t1 AT3G07500 37.736 106 63 2 66 171 34 136 7.03e-12 66.2
MS.gene86226.t1 AT1G76320 27.891 147 102 3 264 410 402 544 6.14e-11 66.6
MS.gene86226.t1 AT1G76320 27.891 147 102 3 264 410 402 544 6.20e-11 66.6
MS.gene86226.t1 AT1G76320 27.891 147 102 3 264 410 402 544 6.20e-11 66.6

Find 161 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATGACTGGTTCCCATTAT+TGG 0.117147 5.1:+23384745 None:intergenic
AAAGATTTGTATAACTATTT+TGG 0.141248 5.1:-23385948 MS.gene86226:CDS
AAAGTATTCTAAACTAAAAT+TGG 0.189918 5.1:-23384584 MS.gene86226:CDS
TGATGTAATGGATGATATTT+TGG 0.190992 5.1:-23384617 MS.gene86226:CDS
CCTCCTATTCATTCTATAAA+TGG 0.204960 5.1:-23383218 MS.gene86226:CDS
ACTCTCCATCTCAAGCTTTC+TGG 0.209501 5.1:+23386167 None:intergenic
TGGTGATTACAATGAGAATT+TGG 0.226630 5.1:-23386501 MS.gene86226:CDS
GATATTATGAATCTTGATTT+TGG 0.234560 5.1:-23386425 MS.gene86226:CDS
CCTCCTATGTATTCTATAAA+TGG 0.259449 5.1:-23383474 MS.gene86226:CDS
TACATTTATTTCCTGCATAT+CGG 0.267468 5.1:-23386094 MS.gene86226:CDS
TATCAAAACATGTCATATAA+TGG 0.273332 5.1:-23386021 MS.gene86226:CDS
TGGTGCATTTGGTGTATATT+TGG 0.274057 5.1:+23383778 None:intergenic
GAATGCTAAGATTCCAATAA+TGG 0.282353 5.1:-23384758 MS.gene86226:CDS
TTTGATGAGGATGGAGATAA+TGG 0.283559 5.1:-23386581 MS.gene86226:CDS
AAAGCACCGGTTTCGTTGTT+AGG 0.291848 5.1:-23386361 MS.gene86226:CDS
ATCAAAACATGTCATATAAT+GGG 0.298640 5.1:-23386020 MS.gene86226:CDS
TTGCTTCTCTAAGCTATCTT+CGG 0.321466 5.1:-23385887 MS.gene86226:CDS
CATTTGTCGGAGGAAGTCTT+TGG 0.348911 5.1:+23383728 None:intergenic
ACATTTATTTCCTGCATATC+GGG 0.352320 5.1:-23386093 MS.gene86226:CDS
GGCGAGCTGGACAATTATTC+CGG 0.353940 5.1:+23386207 None:intergenic
TGTTGTGGTTGCTGCAAAAT+AGG 0.356935 5.1:+23383694 None:intergenic
AGGAAGTCTTTGGCTTGAAT+TGG 0.358471 5.1:+23383738 None:intergenic
TTCTGTTAATCTTGATCTTA+CGG 0.373575 5.1:+23384184 None:intergenic
TGCTTCTCTAAGCTATCTTC+GGG 0.374894 5.1:-23385886 MS.gene86226:CDS
TTGGATTCTTGTGTAATAGA+TGG 0.384545 5.1:-23386482 MS.gene86226:CDS
TGGCCATTTATAGAATGAAT+AGG 0.387122 5.1:+23383215 None:intergenic
TGTGGTGGTTGCTGTAAAAT+AGG 0.389749 5.1:+23383185 None:intergenic
CATATTCAGCAGCATGTGAT+AGG 0.389828 5.1:-23384678 MS.gene86226:CDS
GCACTATCCAATGTATCATT+TGG 0.397797 5.1:-23383261 MS.gene86226:CDS
TCTGATCCGGTGTTGATAAC+TGG 0.398541 5.1:-23385102 MS.gene86226:CDS
GACCTTTGTTCATGACTTGT+TGG 0.399009 5.1:+23383017 None:intergenic
CATAATAGCTTGACTTTCAT+CGG 0.403705 5.1:+23383813 None:intergenic
GCTGTAAAATAAGAGGAGTT+TGG 0.403860 5.1:+23383451 None:intergenic
TAGTGCATTTGGTGTACATT+TGG 0.404990 5.1:+23383534 None:intergenic
GCTGTAAAATAGGAGTAGTT+TGG 0.409130 5.1:+23383195 None:intergenic
AATGCTAAGATTCCAATAAT+GGG 0.412224 5.1:-23384757 MS.gene86226:CDS
AGGAAGTACTTGGCTAGAAT+TGG 0.412632 5.1:+23383494 None:intergenic
CAGTTTGTGTGCAATAGAAA+TGG 0.414838 5.1:-23386290 MS.gene86226:CDS
TGCAAAGTTGCAGCTGAAAG+TGG 0.416677 5.1:-23384652 MS.gene86226:CDS
AACATTTGACTAGGGTCAAT+AGG 0.416834 5.1:-23386253 MS.gene86226:CDS
TAGTGCATTTGGTGTACATT+TGG 0.418525 5.1:+23383278 None:intergenic
TCAGAAGGGAACAGACATAT+TGG 0.427242 5.1:-23384538 MS.gene86226:CDS
CTGGTGCTAACCACAATGTT+AGG 0.437522 5.1:-23384432 MS.gene86226:CDS
GGTTGCACTTGAGACATCAT+AGG 0.438936 5.1:+23383299 None:intergenic
GATACATTGGATAGTGCATT+TGG 0.439923 5.1:+23383267 None:intergenic
TCCATTGACACCATTACGCT+TGG 0.440439 5.1:+23384402 None:intergenic
ATCTTTCCTAACAACGAAAC+CGG 0.440915 5.1:+23386355 None:intergenic
ACAATGTCTAGACCTCTTCA+TGG 0.443526 5.1:+23384465 None:intergenic
CAGAATGAAACAAACCATCT+TGG 0.444221 5.1:-23383329 MS.gene86226:CDS
GGTGTATATTTGGCTGCACT+TGG 0.444676 5.1:+23383788 None:intergenic
AAGGCATTGGATGGTGCATT+TGG 0.445253 5.1:+23383767 None:intergenic
AAAGTGTACCGCAGCAAAGT+TGG 0.448110 5.1:-23386332 MS.gene86226:CDS
GGCTGCACTGTTGACATCAT+AGG 0.450005 5.1:+23383555 None:intergenic
TGGCTTGAATTGGCAAAAGA+AGG 0.454963 5.1:+23383748 None:intergenic
TTTATCAATTTGCTTTCCAA+TGG 0.457449 5.1:+23383985 None:intergenic
CCCGATGTTATACGATCTAA+AGG 0.458963 5.1:-23384511 MS.gene86226:CDS
GGGGTACATGGTGGCAAACA+AGG 0.461825 5.1:-23386000 MS.gene86226:CDS
TTATCAATTTGCTTTCCAAT+GGG 0.465405 5.1:+23383986 None:intergenic
AAGTGTACCGCAGCAAAGTT+GGG 0.465894 5.1:-23386331 MS.gene86226:CDS
TCCGGTGTTGATAACTGGTC+TGG 0.467260 5.1:-23385097 MS.gene86226:CDS
ATCCATTCCATTCCCATGAC+TGG 0.470611 5.1:+23384732 None:intergenic
AAGTATTCTAAACTAAAATT+GGG 0.472379 5.1:-23384583 MS.gene86226:CDS
CCAATAATGGGAACCAGTCA+TGG 0.474453 5.1:-23384745 MS.gene86226:CDS
GCGAGCTGGACAATTATTCC+GGG 0.478461 5.1:+23386208 None:intergenic
CCCAAGCGTAATGGTGTCAA+TGG 0.483260 5.1:-23384403 MS.gene86226:CDS
CATCATAGGTTGGCTTGCAA+TGG 0.483995 5.1:+23383569 None:intergenic
GATGCATTGGATAGTGCATT+TGG 0.485433 5.1:+23383523 None:intergenic
TCCGTCAATCCCCGATATGC+AGG 0.486078 5.1:+23386083 None:intergenic
TAAGAGTAATACCTCTGATC+CGG 0.486946 5.1:-23385115 MS.gene86226:CDS
CAAGCAGTTTGATCTTGAAA+AGG 0.487339 5.1:-23384789 MS.gene86226:CDS
AGACATTGTTCAAATTGTAC+TGG 0.487977 5.1:-23384451 MS.gene86226:CDS
TCTAGAGACCACGATGTCTC+TGG 0.494750 5.1:-23386521 MS.gene86226:CDS
ATAATAGCTTGACTTTCATC+GGG 0.495573 5.1:+23383814 None:intergenic
CAAAGACTTCCTCCGACAAA+TGG 0.497442 5.1:-23383727 MS.gene86226:CDS
TCCTGCATATCGGGGATTGA+CGG 0.498073 5.1:-23386084 MS.gene86226:CDS
TTGATGAGGATGGAGATAAT+GGG 0.498718 5.1:-23386580 MS.gene86226:CDS
ATTAATTGTTCATGACCAAC+TGG 0.500633 5.1:-23384992 MS.gene86226:CDS
GGAGGAGTTTGACCATTTGT+CGG 0.500705 5.1:+23383715 None:intergenic
CAATTTGCTTTCCAATGGGA+TGG 0.513898 5.1:+23383990 None:intergenic
ATGGGTATTGATATTAAAGA+TGG 0.513994 5.1:-23385918 MS.gene86226:CDS
CCATTGACACCATTACGCTT+GGG 0.514811 5.1:+23384403 None:intergenic
CCGATGTTATACGATCTAAA+GGG 0.516186 5.1:-23384510 MS.gene86226:CDS
TGGCCATTTATAGAATACAT+AGG 0.516234 5.1:+23383471 None:intergenic
TAACGTATACGAAGGCGAGC+TGG 0.516616 5.1:+23386194 None:intergenic
CCTTTAGATCGTATAACATC+GGG 0.520619 5.1:+23384511 None:intergenic
TCATAGGTTGACTTCCAAGA+TGG 0.521243 5.1:+23383315 None:intergenic
CTAATATGATAGAGCTTGCT+CGG 0.521561 5.1:-23384708 MS.gene86226:CDS
GCAAAGTTGCAGCTGAAAGT+GGG 0.522215 5.1:-23384651 MS.gene86226:CDS
AATACATAGGAGGAAGTACT+TGG 0.524323 5.1:+23383484 None:intergenic
GATGAGCCGTTAGTAGTTCG+TGG 0.524704 5.1:+23383055 None:intergenic
AAGCAATTCTAATCTGAAGA+AGG 0.526180 5.1:-23383863 MS.gene86226:CDS
ATTGTTGGTTATTATGCAGA+TGG 0.526442 5.1:-23386630 None:intergenic
AATGAATAGGAGGAAGTACT+TGG 0.526943 5.1:+23383228 None:intergenic
ATGTGTGGTCAAAGTTATGC+AGG 0.527030 5.1:+23385175 None:intergenic
CATAATATCATCCGCCGTCA+TGG 0.531051 5.1:+23386439 None:intergenic
CTGGACAATTATTCCGGGTG+AGG 0.532213 5.1:+23386213 None:intergenic
TAGGTTGGCTTGCAATGGGA+TGG 0.532929 5.1:+23383574 None:intergenic
ATTCTTGTGTAATAGATGGT+AGG 0.533148 5.1:-23386478 MS.gene86226:CDS
ACTTGGCTAGAATTGGCAAA+AGG 0.533753 5.1:+23383501 None:intergenic
TTCTATACTCTCTCAATGTG+TGG 0.535446 5.1:+23385160 None:intergenic
ATCATAGGTTGGCTTGCAAT+GGG 0.536598 5.1:+23383570 None:intergenic
AATGTAGTTAACCATCCCAT+TGG 0.537897 5.1:-23384001 MS.gene86226:CDS
GAATTGGCAAAAGGATGCAT+TGG 0.541168 5.1:+23383510 None:intergenic
GAATTAGCCAAATGATACAT+TGG 0.541331 5.1:+23383254 None:intergenic
TAGGACAAAGAAATGTCTAA+AGG 0.545001 5.1:-23384230 MS.gene86226:CDS
GGCGATTTAAACAACATCCA+TGG 0.546504 5.1:-23384209 MS.gene86226:CDS
ATGGGAACCAGTCATGGGAA+TGG 0.547754 5.1:-23384739 MS.gene86226:CDS
TATACAATACAAACCTGTTG+TGG 0.551522 5.1:+23383679 None:intergenic
GCACTGTTGACATCATAGGT+TGG 0.554976 5.1:+23383559 None:intergenic
ATGTCATATAATGGGGTACA+TGG 0.556484 5.1:-23386012 MS.gene86226:CDS
GGAATGCCACGAACTACTAA+CGG 0.557402 5.1:-23383061 MS.gene86226:CDS
AGGACATGTCCCAAGCGTAA+TGG 0.564262 5.1:-23384412 MS.gene86226:CDS
GCAGTTTGATCTTGAAAAGG+TGG 0.564852 5.1:-23384786 MS.gene86226:CDS
ACTTATCTTCAACATCCAGT+TGG 0.564933 5.1:+23384977 None:intergenic
ATAAAGACACAAAATTCAGA+AGG 0.565494 5.1:-23384553 MS.gene86226:CDS
TGGACAATTATTCCGGGTGA+GGG 0.565731 5.1:+23386214 None:intergenic
GAGAACATAGACCCCTCACC+CGG 0.567472 5.1:-23386226 MS.gene86226:CDS
AGATCAAGATTAACAGAACA+TGG 0.572591 5.1:-23384179 MS.gene86226:CDS
CCCTTTAGATCGTATAACAT+CGG 0.573562 5.1:+23384510 None:intergenic
GTGTATATTTGGCTGCACTT+GGG 0.573918 5.1:+23383789 None:intergenic
AACCAGTCATGGGAATGGAA+TGG 0.579805 5.1:-23384734 MS.gene86226:CDS
GTTGACAGTATGCAATCGTA+TGG 0.580581 5.1:-23386044 MS.gene86226:CDS
CAATAATGGGAACCAGTCAT+GGG 0.581291 5.1:-23384744 MS.gene86226:CDS
CAAGATTAACAGAACATGGA+AGG 0.582220 5.1:-23384175 MS.gene86226:CDS
CATTTATTTCCTGCATATCG+GGG 0.582815 5.1:-23386092 MS.gene86226:CDS
ATCTTGATCTTACGGAACCA+TGG 0.586359 5.1:+23384192 None:intergenic
GGTGGTTGCTGTAAAATAAG+AGG 0.588777 5.1:+23383444 None:intergenic
GGGTACATGGTGGCAAACAA+GGG 0.589509 5.1:-23385999 MS.gene86226:CDS
GAATTGGCAAAAGAAGGCAT+TGG 0.600371 5.1:+23383754 None:intergenic
TGTTAAGATTAATTCCATGA+CGG 0.600938 5.1:-23386453 MS.gene86226:CDS
CAAAGTTGCAGCTGAAAGTG+GGG 0.602434 5.1:-23384650 MS.gene86226:CDS
TGGCAAAAGAAGGCATTGGA+TGG 0.604905 5.1:+23383758 None:intergenic
CCATTTATAGAATGAATAGG+AGG 0.608139 5.1:+23383218 None:intergenic
TACATGGTGGCAAACAAGGG+TGG 0.611316 5.1:-23385996 MS.gene86226:CDS
CTTCTCTAAGCTATCTTCGG+GGG 0.613173 5.1:-23385884 MS.gene86226:CDS
AGTTATCAACACCGGATCAG+AGG 0.615238 5.1:+23385104 None:intergenic
CGATGTTATACGATCTAAAG+GGG 0.619205 5.1:-23384509 MS.gene86226:CDS
TGTAGATTGTTTGAGTCTCG+CGG 0.625379 5.1:-23384868 MS.gene86226:CDS
CTGGATTATAACGTATACGA+AGG 0.628678 5.1:+23386186 None:intergenic
CCATTTATAGAATACATAGG+AGG 0.630840 5.1:+23383474 None:intergenic
TCCAGACCAGTTATCAACAC+CGG 0.632781 5.1:+23385096 None:intergenic
TGGGACATGTCCTAACATTG+TGG 0.635268 5.1:+23384422 None:intergenic
ATAATCCAGAAAGCTTGAGA+TGG 0.638128 5.1:-23386172 MS.gene86226:CDS
GCTTCTCTAAGCTATCTTCG+GGG 0.639112 5.1:-23385885 MS.gene86226:CDS
AGAAAGCTTGAGATGGAGAG+TGG 0.639417 5.1:-23386165 MS.gene86226:CDS
AAGGGTGGATATGATGCTGT+TGG 0.642784 5.1:-23385981 MS.gene86226:CDS
GGACAATTATTCCGGGTGAG+GGG 0.643647 5.1:+23386215 None:intergenic
GGAGTTTGACCATTTGTCGG+AGG 0.644844 5.1:+23383718 None:intergenic
TAAAGACACAAAATTCAGAA+GGG 0.646077 5.1:-23384552 MS.gene86226:CDS
CTCCAACAAGTCATGAACAA+AGG 0.646798 5.1:-23383019 MS.gene86226:CDS
TATTCTAAACTAAAATTGGG+AGG 0.648453 5.1:-23384580 MS.gene86226:CDS
TATATAATACACACATGTGG+TGG 0.648699 5.1:+23383170 None:intergenic
TCAAAACATGTCATATAATG+GGG 0.649125 5.1:-23386019 MS.gene86226:CDS
TATACAATACAAACCTGTGG+TGG 0.653372 5.1:+23383426 None:intergenic
TCATATAATGGGGTACATGG+TGG 0.662255 5.1:-23386009 MS.gene86226:CDS
ATCGCAAATCAACAGTGATG+AGG 0.662624 5.1:-23384380 MS.gene86226:CDS
TTTGTTTCCCAACTTTGCTG+CGG 0.666357 5.1:+23386324 None:intergenic
ATGATAGAGCTTGCTCGGTG+TGG 0.668531 5.1:-23384703 MS.gene86226:CDS
TAAGATTAATTCCATGACGG+CGG 0.673303 5.1:-23386450 MS.gene86226:CDS
TGTAATCACCAGAGACATCG+TGG 0.695321 5.1:+23386513 None:intergenic
TGTGGTTGCTGCAAAATAGG+AGG 0.703437 5.1:+23383697 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAATGTATATATTTTGA+TGG + chr5.1:23383525-23383544 None:intergenic 10.0%
!!! ATAAATGTATATATTTTGAT+GGG + chr5.1:23383524-23383543 None:intergenic 10.0%
!! AAGTATTCTAAACTAAAATT+GGG - chr5.1:23385046-23385065 MS.gene86226:CDS 15.0%
!! TGATGATTCATTAAAAAAAA+AGG + chr5.1:23385365-23385384 None:intergenic 15.0%
!! TTATATGTAAAATATGAGAA+GGG - chr5.1:23383789-23383808 MS.gene86226:CDS 15.0%
!!! AAAGATTTGTATAACTATTT+TGG - chr5.1:23383681-23383700 MS.gene86226:intron 15.0%
!!! AAAGTATTCTAAACTAAAAT+TGG - chr5.1:23385045-23385064 MS.gene86226:CDS 15.0%
!!! TAAATGTATATATTTTGATG+GGG + chr5.1:23383523-23383542 None:intergenic 15.0%
!!! TAAATTACTATAGACAATTT+TGG + chr5.1:23385535-23385554 None:intergenic 15.0%
!!! TCATATTTTACATATAACAT+AGG + chr5.1:23383786-23383805 None:intergenic 15.0%
!! AAAAATCACCTAAATTGATA+TGG + chr5.1:23385685-23385704 None:intergenic 20.0%
!! AAATAAATACAATCTTCCTT+TGG - chr5.1:23383914-23383933 MS.gene86226:intron 20.0%
!! AATTATTGCCATATCAATTT+AGG - chr5.1:23385674-23385693 MS.gene86226:intron 20.0%
!! ACAAAAATAATATGAGAACA+TGG + chr5.1:23384791-23384810 None:intergenic 20.0%
!! ACATTCTGTAAAAACATAAA+AGG + chr5.1:23385559-23385578 None:intergenic 20.0%
!! ATCAAAACATGTCATATAAT+GGG - chr5.1:23383609-23383628 MS.gene86226:intron 20.0%
!! GTTATATGTAAAATATGAGA+AGG - chr5.1:23383788-23383807 MS.gene86226:CDS 20.0%
!! TATCAAAACATGTCATATAA+TGG - chr5.1:23383608-23383627 MS.gene86226:intron 20.0%
!! TATTATATAATACACACATG+TGG + chr5.1:23386465-23386484 None:intergenic 20.0%
!!! ATGTTTTTACAAAATGTGAA+GGG - chr5.1:23385740-23385759 MS.gene86226:intron 20.0%
!!! ATTCACAAACGATTATTTTT+GGG - chr5.1:23383961-23383980 MS.gene86226:intron 20.0%
!!! GAAATTGAAAAGATTGTTTT+GGG - chr5.1:23383821-23383840 MS.gene86226:CDS 20.0%
!!! GATATTATGAATCTTGATTT+TGG - chr5.1:23383204-23383223 MS.gene86226:CDS 20.0%
!!! GTATTTATTTTTCTTGTATG+TGG + chr5.1:23383904-23383923 None:intergenic 20.0%
!!! TATGTTTTTACAAAATGTGA+AGG - chr5.1:23385739-23385758 MS.gene86226:intron 20.0%
!!! TCAAATTGTTTTTGTAAATC+TGG - chr5.1:23385332-23385351 MS.gene86226:intron 20.0%
!!! TCATGATTTCAGTATTTTTT+TGG - chr5.1:23386547-23386566 MS.gene86226:CDS 20.0%
!!! TCTAACTTTTTTGAATATCT+CGG + chr5.1:23384594-23384613 None:intergenic 20.0%
!!! TGAATTACTAAAGACAATTT+TGG + chr5.1:23385713-23385732 None:intergenic 20.0%
!!! TTCTGATTTTAATTTTGATG+AGG - chr5.1:23383035-23383054 MS.gene86226:CDS 20.0%
!!! TTTTGCATACTTTTGATAAA+AGG + chr5.1:23383250-23383269 None:intergenic 20.0%
! AAACAATAGAGACATATACA+TGG - chr5.1:23384003-23384022 MS.gene86226:CDS 25.0%
! AACAATAGAGACATATACAT+GGG - chr5.1:23384004-23384023 MS.gene86226:CDS 25.0%
! AAGCAAAAAACATTTGACTA+GGG - chr5.1:23383368-23383387 MS.gene86226:intron 25.0%
! AATGCTAAGATTCCAATAAT+GGG - chr5.1:23384872-23384891 MS.gene86226:CDS 25.0%
! ATAAAGACACAAAATTCAGA+AGG - chr5.1:23385076-23385095 MS.gene86226:CDS 25.0%
! CAAAAATAATCGTTTGTGAA+TGG + chr5.1:23383962-23383981 None:intergenic 25.0%
! TAAAGACACAAAATTCAGAA+GGG - chr5.1:23385077-23385096 MS.gene86226:CDS 25.0%
! TACATTTATTTCCTGCATAT+CGG - chr5.1:23383535-23383554 MS.gene86226:CDS 25.0%
! TATTATACAATACAAACCTG+TGG + chr5.1:23385956-23385975 None:intergenic 25.0%
! TATTCTAAACTAAAATTGGG+AGG - chr5.1:23385049-23385068 MS.gene86226:CDS 25.0%
! TCAAAACATGTCATATAATG+GGG - chr5.1:23383610-23383629 MS.gene86226:intron 25.0%
! TGTTAAGATTAATTCCATGA+CGG - chr5.1:23383176-23383195 MS.gene86226:intron 25.0%
! TTCTGTTAATCTTGATCTTA+CGG + chr5.1:23385448-23385467 None:intergenic 25.0%
!! ATGGAAAAAGTTTTCTTCTT+TGG + chr5.1:23385148-23385167 None:intergenic 25.0%
!! CATTCACAAACGATTATTTT+TGG - chr5.1:23383960-23383979 MS.gene86226:intron 25.0%
!! GGAAATTGAAAAGATTGTTT+TGG - chr5.1:23383820-23383839 MS.gene86226:CDS 25.0%
!! TTATCAATTTGCTTTCCAAT+GGG + chr5.1:23385646-23385665 None:intergenic 25.0%
!! TTTATCAATTTGCTTTCCAA+TGG + chr5.1:23385647-23385666 None:intergenic 25.0%
!!! AACAATCACATAAGTTGATA+AGG + chr5.1:23385507-23385526 None:intergenic 25.0%
!!! AACTATTTTGGCAAGAAAAT+GGG - chr5.1:23383693-23383712 MS.gene86226:CDS 25.0%
!!! ATGGGTATTGATATTAAAGA+TGG - chr5.1:23383711-23383730 MS.gene86226:CDS 25.0%
!!! CATCATTTTTAACATCTTGT+AGG - chr5.1:23385380-23385399 MS.gene86226:intron 25.0%
!!! CGAAATGATTTTTTGTGTTA+TGG - chr5.1:23383858-23383877 MS.gene86226:CDS 25.0%
!!! GATTTTAATTTTGATGAGGA+TGG - chr5.1:23383039-23383058 MS.gene86226:CDS 25.0%
!!! TAACTATTTTGGCAAGAAAA+TGG - chr5.1:23383692-23383711 MS.gene86226:intron 25.0%
!!! TACTTTCGAGATAAGTTTTT+TGG - chr5.1:23384347-23384366 MS.gene86226:CDS 25.0%
!!! TATGTTTTTACAGAATGTGA+AGG - chr5.1:23385561-23385580 MS.gene86226:intron 25.0%
!!! TGATGTAATGGATGATATTT+TGG - chr5.1:23385012-23385031 MS.gene86226:CDS 25.0%
!!! TTCACAAACGATTATTTTTG+GGG - chr5.1:23383962-23383981 MS.gene86226:intron 25.0%
AAAACTCTTCAAATTCCTCT+GGG + chr5.1:23384241-23384260 None:intergenic 30.0%
AAGCAATTCTAATCTGAAGA+AGG - chr5.1:23385766-23385785 MS.gene86226:intron 30.0%
ACATTTATTTCCTGCATATC+GGG - chr5.1:23383536-23383555 MS.gene86226:CDS 30.0%
AGACATTGTTCAAATTGTAC+TGG - chr5.1:23385178-23385197 MS.gene86226:CDS 30.0%
AGATCAAGATTAACAGAACA+TGG - chr5.1:23385450-23385469 MS.gene86226:intron 30.0%
ATAATAGCTTGACTTTCATC+GGG + chr5.1:23385818-23385837 None:intergenic 30.0%
ATACGAGAACAAGGAATTAT+GGG - chr5.1:23384316-23384335 MS.gene86226:intron 30.0%
ATTAATTGTTCATGACCAAC+TGG - chr5.1:23384637-23384656 MS.gene86226:CDS 30.0%
ATTCTTGTGTAATAGATGGT+AGG - chr5.1:23383151-23383170 MS.gene86226:intron 30.0%
CAACGAAAAATATCGAAAAG+AGG - chr5.1:23384700-23384719 MS.gene86226:CDS 30.0%
CATAATAGCTTGACTTTCAT+CGG + chr5.1:23385819-23385838 None:intergenic 30.0%
CCATTTATAGAATACATAGG+AGG + chr5.1:23386158-23386177 None:intergenic 30.0%
CCATTTATAGAATGAATAGG+AGG + chr5.1:23386414-23386433 None:intergenic 30.0%
CCTCCTATGTATTCTATAAA+TGG - chr5.1:23386155-23386174 MS.gene86226:CDS 30.0%
CCTCCTATTCATTCTATAAA+TGG - chr5.1:23386411-23386430 MS.gene86226:CDS 30.0%
GAAGCAAAAAACATTTGACT+AGG - chr5.1:23383367-23383386 MS.gene86226:intron 30.0%
GAATGCTAAGATTCCAATAA+TGG - chr5.1:23384871-23384890 MS.gene86226:CDS 30.0%
GAATTAGCCAAATGATACAT+TGG + chr5.1:23386378-23386397 None:intergenic 30.0%
TAGGACAAAGAAATGTCTAA+AGG - chr5.1:23385399-23385418 MS.gene86226:intron 30.0%
TATACAATACAAACCTGTTG+TGG + chr5.1:23385953-23385972 None:intergenic 30.0%
TATATAATACACACATGTGG+TGG + chr5.1:23386462-23386481 None:intergenic 30.0%
TGGCCATTTATAGAATACAT+AGG + chr5.1:23386161-23386180 None:intergenic 30.0%
TGGCCATTTATAGAATGAAT+AGG + chr5.1:23386417-23386436 None:intergenic 30.0%
TGGTGATTACAATGAGAATT+TGG - chr5.1:23383128-23383147 MS.gene86226:intron 30.0%
TTGGATTCTTGTGTAATAGA+TGG - chr5.1:23383147-23383166 MS.gene86226:intron 30.0%
TTTCAAGAAAGCAATGTACT+CGG - chr5.1:23384208-23384227 MS.gene86226:CDS 30.0%
! AAACTTATCTCGAAAGTATG+CGG + chr5.1:23384345-23384364 None:intergenic 30.0%
! AATTCGTTTCTAGAAGCAAT+GGG - chr5.1:23384032-23384051 MS.gene86226:CDS 30.0%
! AGAGGAATTTGAAGAGTTTT+GGG - chr5.1:23384241-23384260 MS.gene86226:intron 30.0%
! CAATCTTTTCAATTTCCCAT+TGG + chr5.1:23383817-23383836 None:intergenic 30.0%
!! AGAAAACTTTTTCCATGAAG+AGG - chr5.1:23385152-23385171 MS.gene86226:CDS 30.0%
!! TTCTAAAACATACGAGAACA+AGG - chr5.1:23384307-23384326 MS.gene86226:intron 30.0%
!! TTTTCAAGATCAAACTGCTT+GGG + chr5.1:23384841-23384860 None:intergenic 30.0%
!!! ATAAGTTTTTTGGTCGCATA+CGG - chr5.1:23384357-23384376 MS.gene86226:CDS 30.0%
!!! GGCATGTTTTAATGATGTAA+TGG - chr5.1:23385000-23385019 MS.gene86226:CDS 30.0%
AAACTCTTCAAATTCCTCTG+GGG + chr5.1:23384240-23384259 None:intergenic 35.0%
AACATTTGACTAGGGTCAAT+AGG - chr5.1:23383376-23383395 MS.gene86226:intron 35.0%
AATGTAGTTAACCATCCCAT+TGG - chr5.1:23385628-23385647 MS.gene86226:intron 35.0%
ACTTATCTTCAACATCCAGT+TGG + chr5.1:23384655-23384674 None:intergenic 35.0%
ATAATCCAGAAAGCTTGAGA+TGG - chr5.1:23383457-23383476 MS.gene86226:CDS 35.0%
ATCTTTCCTAACAACGAAAC+CGG + chr5.1:23383277-23383296 None:intergenic 35.0%
CAAAACTCTTCAAATTCCTC+TGG + chr5.1:23384242-23384261 None:intergenic 35.0%
CAAGATTAACAGAACATGGA+AGG - chr5.1:23385454-23385473 MS.gene86226:intron 35.0%
CAAGCAGTTTGATCTTGAAA+AGG - chr5.1:23384840-23384859 MS.gene86226:CDS 35.0%
CAGAATGAAACAAACCATCT+TGG - chr5.1:23386300-23386319 MS.gene86226:CDS 35.0%
CAGTTTGTGTGCAATAGAAA+TGG - chr5.1:23383339-23383358 MS.gene86226:intron 35.0%
CATACGAGAACAAGGAATTA+TGG - chr5.1:23384315-23384334 MS.gene86226:intron 35.0%
CATTTATTTCCTGCATATCG+GGG - chr5.1:23383537-23383556 MS.gene86226:CDS 35.0%
CCCTTTAGATCGTATAACAT+CGG + chr5.1:23385122-23385141 None:intergenic 35.0%
CCGATGTTATACGATCTAAA+GGG - chr5.1:23385119-23385138 MS.gene86226:CDS 35.0%
CCTTTAGATCGTATAACATC+GGG + chr5.1:23385121-23385140 None:intergenic 35.0%
CGATGTTATACGATCTAAAG+GGG - chr5.1:23385120-23385139 MS.gene86226:CDS 35.0%
CTGGATTATAACGTATACGA+AGG + chr5.1:23383446-23383465 None:intergenic 35.0%
GATACATTGGATAGTGCATT+TGG + chr5.1:23386365-23386384 None:intergenic 35.0%
GCACTATCCAATGTATCATT+TGG - chr5.1:23386368-23386387 MS.gene86226:CDS 35.0%
GCTGTAAAATAAGAGGAGTT+TGG + chr5.1:23386181-23386200 None:intergenic 35.0%
GCTGTAAAATAGGAGTAGTT+TGG + chr5.1:23386437-23386456 None:intergenic 35.0%
TAAGAGTAATACCTCTGATC+CGG - chr5.1:23384514-23384533 MS.gene86226:CDS 35.0%
TAAGATTAATTCCATGACGG+CGG - chr5.1:23383179-23383198 MS.gene86226:intron 35.0%
TATACAATACAAACCTGTGG+TGG + chr5.1:23386206-23386225 None:intergenic 35.0%
TGAGCTACAAGGTAACAATT+GGG - chr5.1:23384283-23384302 MS.gene86226:intron 35.0%
TTCTATACTCTCTCAATGTG+TGG + chr5.1:23384472-23384491 None:intergenic 35.0%
TTGATGAGGATGGAGATAAT+GGG - chr5.1:23383049-23383068 MS.gene86226:CDS 35.0%
TTGCTTCTCTAAGCTATCTT+CGG - chr5.1:23383742-23383761 MS.gene86226:CDS 35.0%
TTTGATGAGGATGGAGATAA+TGG - chr5.1:23383048-23383067 MS.gene86226:CDS 35.0%
! ATGTCATATAATGGGGTACA+TGG - chr5.1:23383617-23383636 MS.gene86226:intron 35.0%
! CAAAAGTATGCAAAAAGCAC+CGG - chr5.1:23383255-23383274 MS.gene86226:CDS 35.0%
! CAGAGGAATTTGAAGAGTTT+TGG - chr5.1:23384240-23384259 MS.gene86226:intron 35.0%
! CTAATATGATAGAGCTTGCT+CGG - chr5.1:23384921-23384940 MS.gene86226:CDS 35.0%
! CTTCCTTTGGTAATATTCTC+TGG - chr5.1:23383927-23383946 MS.gene86226:intron 35.0%
! GAATTCGTTTCTAGAAGCAA+TGG - chr5.1:23384031-23384050 MS.gene86226:CDS 35.0%
! GGAGAGTGGTAATTTTTGAA+GGG - chr5.1:23383478-23383497 MS.gene86226:CDS 35.0%
! TCTTTTGTCTTCTTCTGCTT+CGG + chr5.1:23385604-23385623 None:intergenic 35.0%
! TGGAGAGTGGTAATTTTTGA+AGG - chr5.1:23383477-23383496 MS.gene86226:CDS 35.0%
!! AAATATGAGAAGGGTACCAA+TGG - chr5.1:23383798-23383817 MS.gene86226:CDS 35.0%
!! AATACATAGGAGGAAGTACT+TGG + chr5.1:23386148-23386167 None:intergenic 35.0%
!! AATATGAGAAGGGTACCAAT+GGG - chr5.1:23383799-23383818 MS.gene86226:CDS 35.0%
!! AATGAATAGGAGGAAGTACT+TGG + chr5.1:23386404-23386423 None:intergenic 35.0%
!! CTTTTCAAGATCAAACTGCT+TGG + chr5.1:23384842-23384861 None:intergenic 35.0%
!! TAGTGCATTTGGTGTACATT+TGG + chr5.1:23386098-23386117 None:intergenic 35.0%
!! TGGTGCATTTGGTGTATATT+TGG + chr5.1:23385854-23385873 None:intergenic 35.0%
!!! AAAAGATTGTTTTGGGCAGA+TGG - chr5.1:23383828-23383847 MS.gene86226:CDS 35.0%
!!! ATTTTGATGGGGTTAGTTCA+TGG + chr5.1:23383512-23383531 None:intergenic 35.0%
ACAATGTCTAGACCTCTTCA+TGG + chr5.1:23385167-23385186 None:intergenic 40.0%
ACACAGTCTGTGAAATGCTT+TGG + chr5.1:23384129-23384148 None:intergenic 40.0%
ACTTGGCTAGAATTGGCAAA+AGG + chr5.1:23386131-23386150 None:intergenic 40.0%
ATCGCAAATCAACAGTGATG+AGG - chr5.1:23385249-23385268 MS.gene86226:intron 40.0%
ATCTTGATCTTACGGAACCA+TGG + chr5.1:23385440-23385459 None:intergenic 40.0%
ATGTGTGGTCAAAGTTATGC+AGG + chr5.1:23384457-23384476 None:intergenic 40.0%
ATTGATTGCTTCACACTGAG+AGG + chr5.1:23384387-23384406 None:intergenic 40.0%
CACAGTCTGTGAAATGCTTT+GGG + chr5.1:23384128-23384147 None:intergenic 40.0%
CAGCCAGAGAATATTACCAA+AGG + chr5.1:23383933-23383952 None:intergenic 40.0%
CATATTCAGCAGCATGTGAT+AGG - chr5.1:23384951-23384970 MS.gene86226:CDS 40.0%
CCCGATGTTATACGATCTAA+AGG - chr5.1:23385118-23385137 MS.gene86226:CDS 40.0%
CTCCAACAAGTCATGAACAA+AGG - chr5.1:23386610-23386629 MS.gene86226:CDS 40.0%
GAATTGGCAAAAGGATGCAT+TGG + chr5.1:23386122-23386141 None:intergenic 40.0%
GATGCATTGGATAGTGCATT+TGG + chr5.1:23386109-23386128 None:intergenic 40.0%
GCAGTTTGATCTTGAAAAGG+TGG - chr5.1:23384843-23384862 MS.gene86226:CDS 40.0%
GGCGATTTAAACAACATCCA+TGG - chr5.1:23385420-23385439 MS.gene86226:intron 40.0%
GGTGGTTGCTGTAAAATAAG+AGG + chr5.1:23386188-23386207 None:intergenic 40.0%
GTGAGCTACAAGGTAACAAT+TGG - chr5.1:23384282-23384301 MS.gene86226:intron 40.0%
GTGTATATTTGGCTGCACTT+GGG + chr5.1:23385843-23385862 None:intergenic 40.0%
GTTGACAGTATGCAATCGTA+TGG - chr5.1:23383585-23383604 MS.gene86226:CDS 40.0%
TCAGAAGGGAACAGACATAT+TGG - chr5.1:23385091-23385110 MS.gene86226:CDS 40.0%
TGCTTCTCTAAGCTATCTTC+GGG - chr5.1:23383743-23383762 MS.gene86226:CDS 40.0%
TGGCTTGAATTGGCAAAAGA+AGG + chr5.1:23385884-23385903 None:intergenic 40.0%
TGTAGATTGTTTGAGTCTCG+CGG - chr5.1:23384761-23384780 MS.gene86226:CDS 40.0%
TGTGGTGGTTGCTGTAAAAT+AGG + chr5.1:23386191-23386210 None:intergenic 40.0%
TGTTGTGGTTGCTGCAAAAT+AGG + chr5.1:23385938-23385957 None:intergenic 40.0%
TTTGTTTCCCAACTTTGCTG+CGG + chr5.1:23383308-23383327 None:intergenic 40.0%
! CAATAATGGGAACCAGTCAT+GGG - chr5.1:23384885-23384904 MS.gene86226:CDS 40.0%
! CAATTTGCTTTCCAATGGGA+TGG + chr5.1:23385642-23385661 None:intergenic 40.0%
! GAATTGGCAAAAGAAGGCAT+TGG + chr5.1:23385878-23385897 None:intergenic 40.0%
! GACCTTTGTTCATGACTTGT+TGG + chr5.1:23386615-23386634 None:intergenic 40.0%
! TATTTTACAGCAACCACCAC+AGG - chr5.1:23386190-23386209 MS.gene86226:CDS 40.0%
! TATTTTGCAGCAACCACAAC+AGG - chr5.1:23385937-23385956 MS.gene86226:CDS 40.0%
! TCATAGGTTGACTTCCAAGA+TGG + chr5.1:23386317-23386336 None:intergenic 40.0%
! TCATATAATGGGGTACATGG+TGG - chr5.1:23383620-23383639 MS.gene86226:intron 40.0%
! TGATGGTGACATGTCTATGA+GGG - chr5.1:23384082-23384101 MS.gene86226:intron 40.0%
!! AGGAAGTACTTGGCTAGAAT+TGG + chr5.1:23386138-23386157 None:intergenic 40.0%
!! AGGAAGTCTTTGGCTTGAAT+TGG + chr5.1:23385894-23385913 None:intergenic 40.0%
!! ATCATAGGTTGGCTTGCAAT+GGG + chr5.1:23386062-23386081 None:intergenic 40.0%
!! TGATTTTGGCAGTGAAGAAG+AGG - chr5.1:23383218-23383237 MS.gene86226:CDS 40.0%
!!! GATTATTTTTGGGGCTGCTT+TGG - chr5.1:23383971-23383990 MS.gene86226:intron 40.0%
AAAGTGTACCGCAGCAAAGT+TGG - chr5.1:23383297-23383316 MS.gene86226:CDS 45.0%
AACCAGTCATGGGAATGGAA+TGG - chr5.1:23384895-23384914 MS.gene86226:CDS 45.0%
AAGGGTGGATATGATGCTGT+TGG - chr5.1:23383648-23383667 MS.gene86226:intron 45.0%
AAGTGTACCGCAGCAAAGTT+GGG - chr5.1:23383298-23383317 MS.gene86226:CDS 45.0%
ACTCTCCATCTCAAGCTTTC+TGG + chr5.1:23383465-23383484 None:intergenic 45.0%
AGAAAGCTTGAGATGGAGAG+TGG - chr5.1:23383464-23383483 MS.gene86226:CDS 45.0%
AGCATTCTTGTGCAAATGCC+AGG + chr5.1:23384153-23384172 None:intergenic 45.0%
AGTTATCAACACCGGATCAG+AGG + chr5.1:23384528-23384547 None:intergenic 45.0%
ATCCATTCCATTCCCATGAC+TGG + chr5.1:23384900-23384919 None:intergenic 45.0%
CAAAGACTTCCTCCGACAAA+TGG - chr5.1:23385902-23385921 MS.gene86226:CDS 45.0%
CAAAGTTGCAGCTGAAAGTG+GGG - chr5.1:23384979-23384998 MS.gene86226:CDS 45.0%
CATAATATCATCCGCCGTCA+TGG + chr5.1:23383193-23383212 None:intergenic 45.0%
CATTTGTCGGAGGAAGTCTT+TGG + chr5.1:23385904-23385923 None:intergenic 45.0%
CCAAAAGCAGTAGTGACTGA+TGG - chr5.1:23384065-23384084 MS.gene86226:intron 45.0%
CCATTGACACCATTACGCTT+GGG + chr5.1:23385229-23385248 None:intergenic 45.0%
CTTCTCTAAGCTATCTTCGG+GGG - chr5.1:23383745-23383764 MS.gene86226:CDS 45.0%
GCAAAGTTGCAGCTGAAAGT+GGG - chr5.1:23384978-23384997 MS.gene86226:CDS 45.0%
GCACTGTTGACATCATAGGT+TGG + chr5.1:23386073-23386092 None:intergenic 45.0%
GCTTCTCTAAGCTATCTTCG+GGG - chr5.1:23383744-23383763 MS.gene86226:CDS 45.0%
GGAATGCCACGAACTACTAA+CGG - chr5.1:23386568-23386587 MS.gene86226:CDS 45.0%
GGAGGAGTTTGACCATTTGT+CGG + chr5.1:23385917-23385936 None:intergenic 45.0%
GGTGTATATTTGGCTGCACT+TGG + chr5.1:23385844-23385863 None:intergenic 45.0%
GGTTGCACTTGAGACATCAT+AGG + chr5.1:23386333-23386352 None:intergenic 45.0%
GTTGAGATGAGTGAGCTACA+AGG - chr5.1:23384272-23384291 MS.gene86226:intron 45.0%
TCCAGACCAGTTATCAACAC+CGG + chr5.1:23384536-23384555 None:intergenic 45.0%
TCCATTGACACCATTACGCT+TGG + chr5.1:23385230-23385249 None:intergenic 45.0%
TGCAAAGTTGCAGCTGAAAG+TGG - chr5.1:23384977-23384996 MS.gene86226:CDS 45.0%
TGGACAATTATTCCGGGTGA+GGG + chr5.1:23383418-23383437 None:intergenic 45.0%
TGGGACATGTCCTAACATTG+TGG + chr5.1:23385210-23385229 None:intergenic 45.0%
TGTAATCACCAGAGACATCG+TGG + chr5.1:23383119-23383138 None:intergenic 45.0%
TGTGGTTGCTGCAAAATAGG+AGG + chr5.1:23385935-23385954 None:intergenic 45.0%
! CCAATAATGGGAACCAGTCA+TGG - chr5.1:23384884-23384903 MS.gene86226:CDS 45.0%
! CCATCAGTCACTACTGCTTT+TGG + chr5.1:23384068-23384087 None:intergenic 45.0%
! CCATGACTGGTTCCCATTAT+TGG + chr5.1:23384887-23384906 None:intergenic 45.0%
! CTGATGGTGACATGTCTATG+AGG - chr5.1:23384081-23384100 MS.gene86226:intron 45.0%
! CTGGTGCTAACCACAATGTT+AGG - chr5.1:23385197-23385216 MS.gene86226:CDS 45.0%
!! AAAGCACCGGTTTCGTTGTT+AGG - chr5.1:23383268-23383287 MS.gene86226:CDS 45.0%
!! AAGGCATTGGATGGTGCATT+TGG + chr5.1:23385865-23385884 None:intergenic 45.0%
!! CATCATAGGTTGGCTTGCAA+TGG + chr5.1:23386063-23386082 None:intergenic 45.0%
!! TCTGATCCGGTGTTGATAAC+TGG - chr5.1:23384527-23384546 MS.gene86226:CDS 45.0%
!! TGGCAAAAGAAGGCATTGGA+TGG + chr5.1:23385874-23385893 None:intergenic 45.0%
!!! GATTGTTTTGGGCAGATGGA+TGG - chr5.1:23383832-23383851 MS.gene86226:CDS 45.0%
AGGACATGTCCCAAGCGTAA+TGG - chr5.1:23385217-23385236 MS.gene86226:intron 50.0%
ATGGGAACCAGTCATGGGAA+TGG - chr5.1:23384890-23384909 MS.gene86226:CDS 50.0%
CATTTCACAGACTGTGTGCC+TGG - chr5.1:23384132-23384151 MS.gene86226:intron 50.0%
CTGGACAATTATTCCGGGTG+AGG + chr5.1:23383419-23383438 None:intergenic 50.0%
GATGAGCCGTTAGTAGTTCG+TGG + chr5.1:23386577-23386596 None:intergenic 50.0%
GCGAGCTGGACAATTATTCC+GGG + chr5.1:23383424-23383443 None:intergenic 50.0%
GGACAATTATTCCGGGTGAG+GGG + chr5.1:23383417-23383436 None:intergenic 50.0%
GGAGTTTGACCATTTGTCGG+AGG + chr5.1:23385914-23385933 None:intergenic 50.0%
GGCGAGCTGGACAATTATTC+CGG + chr5.1:23383425-23383444 None:intergenic 50.0%
GGCTGCACTGTTGACATCAT+AGG + chr5.1:23386077-23386096 None:intergenic 50.0%
GGGTACATGGTGGCAAACAA+GGG - chr5.1:23383630-23383649 MS.gene86226:intron 50.0%
TAACGTATACGAAGGCGAGC+TGG + chr5.1:23383438-23383457 None:intergenic 50.0%
TACATGGTGGCAAACAAGGG+TGG - chr5.1:23383633-23383652 MS.gene86226:intron 50.0%
TCCTGCATATCGGGGATTGA+CGG - chr5.1:23383545-23383564 MS.gene86226:CDS 50.0%
TCTAAGCTATCTTCGGGGGA+AGG - chr5.1:23383749-23383768 MS.gene86226:CDS 50.0%
TCTAGAGACCACGATGTCTC+TGG - chr5.1:23383108-23383127 MS.gene86226:intron 50.0%
! ATGATAGAGCTTGCTCGGTG+TGG - chr5.1:23384926-23384945 MS.gene86226:CDS 50.0%
! CCCAAGCGTAATGGTGTCAA+TGG - chr5.1:23385226-23385245 MS.gene86226:intron 50.0%
!! TAGGTTGGCTTGCAATGGGA+TGG + chr5.1:23386058-23386077 None:intergenic 50.0%
!! TCCGGTGTTGATAACTGGTC+TGG - chr5.1:23384532-23384551 MS.gene86226:CDS 50.0%
AAGCTATCTTCGGGGGAAGG+CGG - chr5.1:23383752-23383771 MS.gene86226:CDS 55.0%
GAGAACATAGACCCCTCACC+CGG - chr5.1:23383403-23383422 MS.gene86226:intron 55.0%
GGGGTACATGGTGGCAAACA+AGG - chr5.1:23383629-23383648 MS.gene86226:intron 55.0%
GTACTCGGATTTCACCCCAG+AGG - chr5.1:23384223-23384242 MS.gene86226:CDS 55.0%
TCCGTCAATCCCCGATATGC+AGG + chr5.1:23383549-23383568 None:intergenic 55.0%
AGCTATCTTCGGGGGAAGGC+GGG - chr5.1:23383753-23383772 MS.gene86226:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 23383012 23386639 23383012 ID=MS.gene86226
chr5.1 mRNA 23383012 23386639 23383012 ID=MS.gene86226.t1;Parent=MS.gene86226
chr5.1 exon 23385857 23386639 23385857 ID=MS.gene86226.t1.exon1;Parent=MS.gene86226.t1
chr5.1 CDS 23385857 23386639 23385857 ID=cds.MS.gene86226.t1;Parent=MS.gene86226.t1
chr5.1 exon 23384333 23385217 23384333 ID=MS.gene86226.t1.exon2;Parent=MS.gene86226.t1
chr5.1 CDS 23384333 23385217 23384333 ID=cds.MS.gene86226.t1;Parent=MS.gene86226.t1
chr5.1 exon 23384163 23384249 23384163 ID=MS.gene86226.t1.exon3;Parent=MS.gene86226.t1
chr5.1 CDS 23384163 23384249 23384163 ID=cds.MS.gene86226.t1;Parent=MS.gene86226.t1
chr5.1 exon 23383985 23384077 23383985 ID=MS.gene86226.t1.exon4;Parent=MS.gene86226.t1
chr5.1 CDS 23383985 23384077 23383985 ID=cds.MS.gene86226.t1;Parent=MS.gene86226.t1
chr5.1 exon 23383693 23383890 23383693 ID=MS.gene86226.t1.exon5;Parent=MS.gene86226.t1
chr5.1 CDS 23383693 23383890 23383693 ID=cds.MS.gene86226.t1;Parent=MS.gene86226.t1
chr5.1 exon 23383440 23383607 23383440 ID=MS.gene86226.t1.exon6;Parent=MS.gene86226.t1
chr5.1 CDS 23383440 23383607 23383440 ID=cds.MS.gene86226.t1;Parent=MS.gene86226.t1
chr5.1 exon 23383184 23383348 23383184 ID=MS.gene86226.t1.exon7;Parent=MS.gene86226.t1
chr5.1 CDS 23383184 23383348 23383184 ID=cds.MS.gene86226.t1;Parent=MS.gene86226.t1
chr5.1 exon 23383012 23383092 23383012 ID=MS.gene86226.t1.exon8;Parent=MS.gene86226.t1
chr5.1 CDS 23383012 23383092 23383012 ID=cds.MS.gene86226.t1;Parent=MS.gene86226.t1
Gene Sequence

>MS.gene86226

ATGCAGATGGATAGCTCTGATGATTCTGATTTTAATTTTGATGAGGATGGAGATAATGGGTATTCTACTTCTAGTGAAGACGATGAAAGTATAGAGTCTAGAGACCACGATGTCTCTGGTGATTACAATGAGAATTTGGATTCTTGTGTAATAGATGGTAGGTTTGTTAAGATTAATTCCATGACGGCGGATGATATTATGAATCTTGATTTTGGCAGTGAAGAAGAGGCTTATGCCTTTTATCAAAAGTATGCAAAAAGCACCGGTTTCGTTGTTAGGAAAGATAAAGTGTACCGCAGCAAAGTTGGGAAACAAATTGTAATGCGACAGTTTGTGTGCAATAGAAATGGATTAAGAAGCAAAAAACATTTGACTAGGGTCAATAGGAAAAGAGAACATAGACCCCTCACCCGGAATAATTGTCCAGCTCGCCTTCGTATACGTTATAATCCAGAAAGCTTGAGATGGAGAGTGGTAATTTTTGAAGGGTGTCATAACCATGAACTAACCCCATCAAAATATATACATTTATTTCCTGCATATCGGGGATTGACGGATGCTGATAAAGCTCAAGTTGACAGTATGCAATCGTATGGTATCAAAACATGTCATATAATGGGGTACATGGTGGCAAACAAGGGTGGATATGATGCTGTTGGTTTTACGAAGAAAGATTTGTATAACTATTTTGGCAAGAAAATGGGTATTGATATTAAAGATGGTGATGTTGTTGCTTCTCTAAGCTATCTTCGGGGGAAGGCGGGCTTAGATCCTATGTTATATGTAAAATATGAGAAGGGTACCAATGGGAAATTGAAAAGATTGTTTTGGGCAGATGGATGGAGTCGAAATGATTTTTTGTGTTATGGCGATGTTATTGCATTTGATACCACATACAAGAAAAATAAATACAATCTTCCTTTGGTAATATTCTCTGGCTGCAACAACCATTCACAAACGATTATTTTTGGGGCTGCTTTGGTTTCAGATGAAACAATAGAGACATATACATGGGTGTTGAATTCGTTTCTAGAAGCAATGGGAAATCGACAACCAAAAGCAGTAGTGACTGATGGTGACATGTCTATGAGGGACGCAGTAAAAGTTGTTTTTCCCAAAGCATTTCACAGACTGTGTGCCTGGCATTTGCACAAGAATGCTTATGAAAATGTTAAAAATAAAGAATTTTTGACAAATTTCAAGAAAGCAATGTACTCGGATTTCACCCCAGAGGAATTTGAAGAGTTTTGGGCAAGTGCTGTTGAGATGAGTGAGCTACAAGGTAACAATTGGGTTTCTAAAACATACGAGAACAAGGAATTATGGGCAACCGCATACTTTCGAGATAAGTTTTTTGGTCGCATACGGACAACCTCTCAGTGTGAAGCAATCAATTCAGTAGTCAAGAAATATGTCAGAAAGAAATGCAGCATTTATGAGTTCCTGCATAACTTTGACCACACATTGAGAGAGTATAGAAACAACGAACATGTTGCTGATTTTAAGAGTAATACCTCTGATCCGGTGTTGATAACTGGTCTGGATAATATTGAGAAAGATGCTGCAAAGATTTATACATCCGAGATATTCAAAAAAGTTAGAAAACACATTGTCAAGTCTTCTGCATTAATTGTTCATGACCAACTGGATGTTGAAGATAAGTCAATGTTTAAGTTAAGCAAAACATGCAACGAAAAATATCGAAAAGAGGTTGTATACGACATTGCAGACTCTTCTTTTCACTGTGAATGTAGATTGTTTGAGTCTCGCGGATTTCCATGTTCTCATATTATTTTTGTCATGAAAGTAGTGCACATTGATCATATCCCAAGCAGTTTGATCTTGAAAAGGTGGACTAAGAATGCTAAGATTCCAATAATGGGAACCAGTCATGGGAATGGAATGGATTCTAATATGATAGAGCTTGCTCGGTGTGGTGCATATTCAGCAGCATGTGATAGGTTTTGCAAAGTTGCAGCTGAAAGTGGGGCATGTTTTAATGATGTAATGGATGATATTTTGGAGCTTACTGAAAAGTATTCTAAACTAAAATTGGGAGGTGCTATAAAGACACAAAATTCAGAAGGGAACAGACATATTGGTGATCCCGATGTTATACGATCTAAAGGGGCTCCAAAGAAGAAAACTTTTTCCATGAAGAGGTCTAGACATTGTTCAAATTGTACTGGTGCTAACCACAATGTTAGGACATGTCCCAAGCGTAATGGTGTCAATGGATCGCAAATCAACAGTGATGAGGAATCTGAGTCAGATGCTACAAAATTTAGCAATGCAGCAGATAATATTGTAAGTGCATCTGTCAAATTGTTTTTGTAAATCTGGTGTTATACCTTTTTTTTTAATGAATCATCATTTTTAACATCTTGTAGGACAAAGAAATGTCTAAAGGCGATTTAAACAACATCCATGGTTCCGTAAGATCAAGATTAACAGAACATGGAAGGCAAATTGATAAAGTGATTGAATCACTACCTTATCAACTTATGTGATTGTTATTTGCCAAAATTGTCTATAGTAATTTATCCTTTTATGTTTTTACAGAATGTGAAGGAAAAAAGTAATTCTAAACCGAAGCAGAAGAAGACAAAAGAGTTGAATGTAGTTAACCATCCCATTGGAAAGCAAATTGATAAAGTGAATAAATTATTGCCATATCAATTTAGGTGATTTTTATTTGCCAAAATTGTCTTTAGTAATTCATCATTTTATGTTTTTACAAAATGTGAAGGGAGAAAGCAATTCTAATCTGAAGAAGGCAAAAGAGTTGAATGAAATTAGACATCCCGATGAAAGTCAAGCTATTATGTCCCAAGTGCAGCCAAATATACACCAAATGCACCATCCAATGCCTTCTTTTGCCAATTCAAGCCAAAGACTTCCTCCGACAAATGGTCAAACTCCTCCTATTTTGCAGCAACCACAACAGGTTTGTATTGTATAATATTACTTTGCTGTTATAATGTTAAATTTATGACAAATTTATCTAAAATTTGAAAATGTCATAATTTCAGAATGAAATAAACCATCCCATTGCAAGCCAACCTATGATGTCAACAGTGCAGCCAAATGTACACCAAATGCACTATCCAATGCATCCTTTTGCCAATTCTAGCCAAGTACTTCCTCCTATGTATTCTATAAATGGCCAAACTCCTCTTATTTTACAGCAACCACCACAGGTTTGTATTGTATAATATGTTAATGATTTTTCTGCTATTATGTTAATTTTATGACATATGTATATGAAATTTGAAAATGTCGTCATTTCAGAATGAAACAAACCATCTTGGAAGTCAACCTATGATGTCTCAAGTGCAACCAAATGTACACCAAATGCACTATCCAATGTATCATTTGGCTAATTCAAGCCAAGTACTTCCTCCTATTCATTCTATAAATGGCCAAACTACTCCTATTTTACAGCAACCACCACATGTGTGTATTATATAATATAGTATTGCTTATGCTGTTATTATGTCATTTTTATGACAAATTTATCTAAAATTTGAATATGTCATGATTTCAGTATTTTTTTGGAATGCCACGAACTACTAACGGCTCATCTTGTATTGACATGCTCCAACAAGTCATGAACAAAGGTCGTTAA
Protein sequence

>MS.gene86226.t1

MQMDSSDDSDFNFDEDGDNGYSTSSEDDESIESRDHDVSGDYNENLDSCVIDGRFVKINSMTADDIMNLDFGSEEEAYAFYQKYAKSTGFVVRKDKVYRSKVGKQIVMRQFVCNRNGLRSKKHLTRVNRKREHRPLTRNNCPARLRIRYNPESLRWRVVIFEGCHNHELTPSKYIHLFPAYRGLTDADKAQVDSMQSYGIKTCHIMGYMVANKGGYDAVGFTKKDLYNYFGKKMGIDIKDGDVVASLSYLRGKAGLDPMLYKCSIYEFLHNFDHTLREYRNNEHVADFKSNTSDPVLITGLDNIEKDAAKIYTSEIFKKVRKHIVKSSALIVHDQLDVEDKSMFKLSKTCNEKYRKEVVYDIADSSFHCECRLFESRGFPCSHIIFVMKVVHIDHIPSSLILKRWTKNAKIPIMGTSHGNGMDSNMIELARCGAYSAACDRFCKVAAESGACFNDVMDDILELTEKYSKLKLGGAIKTQNSEGNRHIGDPDVIRSKGAPKKKTFSMKRSRHCSNCTGANHNVRTCPKRNGVNGSQINSDEESESDATKFSNAADNIDKEMSKGDLNNIHGSVRSRLTEHGRQIDKNVKEKSNSKPKQKKTKELNVVNHPIGKQIDKGESNSNLKKAKELNEIRHPDESQAIMSQVQPNIHQMHHPMPSFANSSQRLPPTNGQTPPILQQPQQNEINHPIASQPMMSTVQPNVHQMHYPMHPFANSSQVLPPMYSINGQTPLILQQPPQNETNHLGSQPMMSQVQPNVHQMHYPMYHLANSSQVLPPIHSINGQTTPILQQPPHYFFGMPRTTNGSSCIDMLQQVMNKGR