AlfalfaGEDB | MS.gene86226

M. sativa cultivar XinJiangDaYe / MS.gene86226

KEGG: -
GO: GO:0003674 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86226.t1	XP_029126074.1	40.6	828	262	7	3	613	1	815	2.30E-147	533.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86226.t1	Q9SZL8	31.4	255	152	7	16	251	8	258	6.9e-20	100.9
MS.gene86226.t1	Q9SZL8	23.2	233	177	2	264	496	489	719	1.4e-12	76.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86226.t1	A0A396J342	35.2	731	254	4	1	515	8	734	2.6e-108	402.9

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene86226.t1	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86226.t1	MTR_7g446520	47.710	262	90	5	13	258	32	262	1.05e-63	217
MS.gene86226.t1	MTR_7g028855	52.000	200	91	4	14	209	65	263	9.71e-55	196
MS.gene86226.t1	MTR_7g446480	62.069	116	44	0	143	258	140	255	1.42e-42	158
MS.gene86226.t1	MTR_7g446510	61.207	116	45	0	143	258	155	270	8.05e-41	153
MS.gene86226.t1	MTR_1g070355	34.201	269	159	6	1	261	9	267	9.56e-37	146
MS.gene86226.t1	MTR_5g076070	32.083	240	156	3	38	277	26	258	1.33e-34	134
MS.gene86226.t1	MTR_6g022080	31.250	176	118	2	55	228	73	247	4.91e-25	105
MS.gene86226.t1	MTR_0402s0040	31.111	180	113	3	67	242	28	200	1.79e-20	96.3
MS.gene86226.t1	MTR_6g051425	28.796	191	133	3	73	261	25	214	9.62e-20	92.0
MS.gene86226.t1	MTR_8g468460	50.526	95	46	1	275	369	12	105	9.92e-20	85.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86226.t1	AT4G38180	30.566	265	161	7	16	261	8	268	5.44e-22	102
MS.gene86226.t1	AT4G38180	23.478	230	174	2	267	496	492	719	3.01e-14	77.4
MS.gene86226.t1	AT1G10240	33.520	179	110	5	71	243	54	229	4.46e-18	89.7
MS.gene86226.t1	AT1G10240	33.520	179	110	5	71	243	54	229	4.46e-18	89.7
MS.gene86226.t1	AT4G15090	28.627	255	164	9	262	508	463	707	2.22e-16	84.3
MS.gene86226.t1	AT4G15090	27.803	223	148	6	69	290	55	265	2.19e-14	77.8
MS.gene86226.t1	AT4G15090	28.627	255	164	9	262	508	463	707	2.22e-16	84.3
MS.gene86226.t1	AT4G15090	27.803	223	148	6	69	290	55	265	2.19e-14	77.8
MS.gene86226.t1	AT4G15090	28.627	255	164	9	262	508	463	707	2.22e-16	84.3
MS.gene86226.t1	AT4G15090	27.803	223	148	6	69	290	55	265	2.19e-14	77.8

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Find 161 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGATTTGTATAACTATTT+TGG	0.141248	5.1:-23385948	MS.gene86226:CDS
AAAGCACCGGTTTCGTTGTT+AGG	0.291848	5.1:-23386361	MS.gene86226:CDS
AAAGTATTCTAAACTAAAAT+TGG	0.189918	5.1:-23384584	MS.gene86226:CDS
AAAGTGTACCGCAGCAAAGT+TGG	0.448110	5.1:-23386332	MS.gene86226:CDS
AACATTTGACTAGGGTCAAT+AGG	0.416834	5.1:-23386253	MS.gene86226:CDS
AACCAGTCATGGGAATGGAA+TGG	0.579805	5.1:-23384734	MS.gene86226:CDS
AAGCAATTCTAATCTGAAGA+AGG	0.526180	5.1:-23383863	MS.gene86226:CDS
AAGGCATTGGATGGTGCATT+TGG	0.445253	5.1:+23383767	None:intergenic
AAGGGTGGATATGATGCTGT+TGG	0.642784	5.1:-23385981	MS.gene86226:CDS
AAGTATTCTAAACTAAAATT+GGG	0.472379	5.1:-23384583	MS.gene86226:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACTCTTCAAATTCCTCT+GGG	+	chr5.1:23384241-23384260	None:intergenic	30.0%
AAGCAATTCTAATCTGAAGA+AGG	-	chr5.1:23385766-23385785	MS.gene86226:intron	30.0%
ACATTTATTTCCTGCATATC+GGG	-	chr5.1:23383536-23383555	MS.gene86226:CDS	30.0%
AGACATTGTTCAAATTGTAC+TGG	-	chr5.1:23385178-23385197	MS.gene86226:CDS	30.0%
AGATCAAGATTAACAGAACA+TGG	-	chr5.1:23385450-23385469	MS.gene86226:intron	30.0%
ATAATAGCTTGACTTTCATC+GGG	+	chr5.1:23385818-23385837	None:intergenic	30.0%
ATACGAGAACAAGGAATTAT+GGG	-	chr5.1:23384316-23384335	MS.gene86226:intron	30.0%
ATTAATTGTTCATGACCAAC+TGG	-	chr5.1:23384637-23384656	MS.gene86226:CDS	30.0%
ATTCTTGTGTAATAGATGGT+AGG	-	chr5.1:23383151-23383170	MS.gene86226:intron	30.0%
CAACGAAAAATATCGAAAAG+AGG	-	chr5.1:23384700-23384719	MS.gene86226:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	23383012	23386639	23383012	ID=MS.gene86226
chr5.1	mRNA	23383012	23386639	23383012	ID=MS.gene86226.t1;Parent=MS.gene86226
chr5.1	exon	23385857	23386639	23385857	ID=MS.gene86226.t1.exon1;Parent=MS.gene86226.t1
chr5.1	CDS	23385857	23386639	23385857	ID=cds.MS.gene86226.t1;Parent=MS.gene86226.t1
chr5.1	exon	23384333	23385217	23384333	ID=MS.gene86226.t1.exon2;Parent=MS.gene86226.t1
chr5.1	CDS	23384333	23385217	23384333	ID=cds.MS.gene86226.t1;Parent=MS.gene86226.t1
chr5.1	exon	23384163	23384249	23384163	ID=MS.gene86226.t1.exon3;Parent=MS.gene86226.t1
chr5.1	CDS	23384163	23384249	23384163	ID=cds.MS.gene86226.t1;Parent=MS.gene86226.t1
chr5.1	exon	23383985	23384077	23383985	ID=MS.gene86226.t1.exon4;Parent=MS.gene86226.t1
chr5.1	CDS	23383985	23384077	23383985	ID=cds.MS.gene86226.t1;Parent=MS.gene86226.t1
chr5.1	exon	23383693	23383890	23383693	ID=MS.gene86226.t1.exon5;Parent=MS.gene86226.t1
chr5.1	CDS	23383693	23383890	23383693	ID=cds.MS.gene86226.t1;Parent=MS.gene86226.t1
chr5.1	exon	23383440	23383607	23383440	ID=MS.gene86226.t1.exon6;Parent=MS.gene86226.t1
chr5.1	CDS	23383440	23383607	23383440	ID=cds.MS.gene86226.t1;Parent=MS.gene86226.t1
chr5.1	exon	23383184	23383348	23383184	ID=MS.gene86226.t1.exon7;Parent=MS.gene86226.t1
chr5.1	CDS	23383184	23383348	23383184	ID=cds.MS.gene86226.t1;Parent=MS.gene86226.t1
chr5.1	exon	23383012	23383092	23383012	ID=MS.gene86226.t1.exon8;Parent=MS.gene86226.t1
chr5.1	CDS	23383012	23383092	23383012	ID=cds.MS.gene86226.t1;Parent=MS.gene86226.t1

Gene Sequence

>MS.gene86226

ATGCAGATGGATAGCTCTGATGATTCTGATTTTAATTTTGATGAGGATGGAGATAATGGGTATTCTACTTCTAGTGAAGACGATGAAAGTATAGAGTCTAGAGACCACGATGTCTCTGGTGATTACAATGAGAATTTGGATTCTTGTGTAATAGATGGTAGGTTTGTTAAGATTAATTCCATGACGGCGGATGATATTATGAATCTTGATTTTGGCAGTGAAGAAGAGGCTTATGCCTTTTATCAAAAGTATGCAAAAAGCACCGGTTTCGTTGTTAGGAAAGATAAAGTGTACCGCAGCAAAGTTGGGAAACAAATTGTAATGCGACAGTTTGTGTGCAATAGAAATGGATTAAGAAGCAAAAAACATTTGACTAGGGTCAATAGGAAAAGAGAACATAGACCCCTCACCCGGAATAATTGTCCAGCTCGCCTTCGTATACGTTATAATCCAGAAAGCTTGAGATGGAGAGTGGTAATTTTTGAAGGGTGTCATAACCATGAACTAACCCCATCAAAATATATACATTTATTTCCTGCATATCGGGGATTGACGGATGCTGATAAAGCTCAAGTTGACAGTATGCAATCGTATGGTATCAAAACATGTCATATAATGGGGTACATGGTGGCAAACAAGGGTGGATATGATGCTGTTGGTTTTACGAAGAAAGATTTGTATAACTATTTTGGCAAGAAAATGGGTATTGATATTAAAGATGGTGATGTTGTTGCTTCTCTAAGCTATCTTCGGGGGAAGGCGGGCTTAGATCCTATGTTATATGTAAAATATGAGAAGGGTACCAATGGGAAATTGAAAAGATTGTTTTGGGCAGATGGATGGAGTCGAAATGATTTTTTGTGTTATGGCGATGTTATTGCATTTGATACCACATACAAGAAAAATAAATACAATCTTCCTTTGGTAATATTCTCTGGCTGCAACAACCATTCACAAACGATTATTTTTGGGGCTGCTTTGGTTTCAGATGAAACAATAGAGACATATACATGGGTGTTGAATTCGTTTCTAGAAGCAATGGGAAATCGACAACCAAAAGCAGTAGTGACTGATGGTGACATGTCTATGAGGGACGCAGTAAAAGTTGTTTTTCCCAAAGCATTTCACAGACTGTGTGCCTGGCATTTGCACAAGAATGCTTATGAAAATGTTAAAAATAAAGAATTTTTGACAAATTTCAAGAAAGCAATGTACTCGGATTTCACCCCAGAGGAATTTGAAGAGTTTTGGGCAAGTGCTGTTGAGATGAGTGAGCTACAAGGTAACAATTGGGTTTCTAAAACATACGAGAACAAGGAATTATGGGCAACCGCATACTTTCGAGATAAGTTTTTTGGTCGCATACGGACAACCTCTCAGTGTGAAGCAATCAATTCAGTAGTCAAGAAATATGTCAGAAAGAAATGCAGCATTTATGAGTTCCTGCATAACTTTGACCACACATTGAGAGAGTATAGAAACAACGAACATGTTGCTGATTTTAAGAGTAATACCTCTGATCCGGTGTTGATAACTGGTCTGGATAATATTGAGAAAGATGCTGCAAAGATTTATACATCCGAGATATTCAAAAAAGTTAGAAAACACATTGTCAAGTCTTCTGCATTAATTGTTCATGACCAACTGGATGTTGAAGATAAGTCAATGTTTAAGTTAAGCAAAACATGCAACGAAAAATATCGAAAAGAGGTTGTATACGACATTGCAGACTCTTCTTTTCACTGTGAATGTAGATTGTTTGAGTCTCGCGGATTTCCATGTTCTCATATTATTTTTGTCATGAAAGTAGTGCACATTGATCATATCCCAAGCAGTTTGATCTTGAAAAGGTGGACTAAGAATGCTAAGATTCCAATAATGGGAACCAGTCATGGGAATGGAATGGATTCTAATATGATAGAGCTTGCTCGGTGTGGTGCATATTCAGCAGCATGTGATAGGTTTTGCAAAGTTGCAGCTGAAAGTGGGGCATGTTTTAATGATGTAATGGATGATATTTTGGAGCTTACTGAAAAGTATTCTAAACTAAAATTGGGAGGTGCTATAAAGACACAAAATTCAGAAGGGAACAGACATATTGGTGATCCCGATGTTATACGATCTAAAGGGGCTCCAAAGAAGAAAACTTTTTCCATGAAGAGGTCTAGACATTGTTCAAATTGTACTGGTGCTAACCACAATGTTAGGACATGTCCCAAGCGTAATGGTGTCAATGGATCGCAAATCAACAGTGATGAGGAATCTGAGTCAGATGCTACAAAATTTAGCAATGCAGCAGATAATATTGTAAGTGCATCTGTCAAATTGTTTTTGTAAATCTGGTGTTATACCTTTTTTTTTAATGAATCATCATTTTTAACATCTTGTAGGACAAAGAAATGTCTAAAGGCGATTTAAACAACATCCATGGTTCCGTAAGATCAAGATTAACAGAACATGGAAGGCAAATTGATAAAGTGATTGAATCACTACCTTATCAACTTATGTGATTGTTATTTGCCAAAATTGTCTATAGTAATTTATCCTTTTATGTTTTTACAGAATGTGAAGGAAAAAAGTAATTCTAAACCGAAGCAGAAGAAGACAAAAGAGTTGAATGTAGTTAACCATCCCATTGGAAAGCAAATTGATAAAGTGAATAAATTATTGCCATATCAATTTAGGTGATTTTTATTTGCCAAAATTGTCTTTAGTAATTCATCATTTTATGTTTTTACAAAATGTGAAGGGAGAAAGCAATTCTAATCTGAAGAAGGCAAAAGAGTTGAATGAAATTAGACATCCCGATGAAAGTCAAGCTATTATGTCCCAAGTGCAGCCAAATATACACCAAATGCACCATCCAATGCCTTCTTTTGCCAATTCAAGCCAAAGACTTCCTCCGACAAATGGTCAAACTCCTCCTATTTTGCAGCAACCACAACAGGTTTGTATTGTATAATATTACTTTGCTGTTATAATGTTAAATTTATGACAAATTTATCTAAAATTTGAAAATGTCATAATTTCAGAATGAAATAAACCATCCCATTGCAAGCCAACCTATGATGTCAACAGTGCAGCCAAATGTACACCAAATGCACTATCCAATGCATCCTTTTGCCAATTCTAGCCAAGTACTTCCTCCTATGTATTCTATAAATGGCCAAACTCCTCTTATTTTACAGCAACCACCACAGGTTTGTATTGTATAATATGTTAATGATTTTTCTGCTATTATGTTAATTTTATGACATATGTATATGAAATTTGAAAATGTCGTCATTTCAGAATGAAACAAACCATCTTGGAAGTCAACCTATGATGTCTCAAGTGCAACCAAATGTACACCAAATGCACTATCCAATGTATCATTTGGCTAATTCAAGCCAAGTACTTCCTCCTATTCATTCTATAAATGGCCAAACTACTCCTATTTTACAGCAACCACCACATGTGTGTATTATATAATATAGTATTGCTTATGCTGTTATTATGTCATTTTTATGACAAATTTATCTAAAATTTGAATATGTCATGATTTCAGTATTTTTTTGGAATGCCACGAACTACTAACGGCTCATCTTGTATTGACATGCTCCAACAAGTCATGAACAAAGGTCGTTAA

Protein sequence

>MS.gene86226.t1

MQMDSSDDSDFNFDEDGDNGYSTSSEDDESIESRDHDVSGDYNENLDSCVIDGRFVKINSMTADDIMNLDFGSEEEAYAFYQKYAKSTGFVVRKDKVYRSKVGKQIVMRQFVCNRNGLRSKKHLTRVNRKREHRPLTRNNCPARLRIRYNPESLRWRVVIFEGCHNHELTPSKYIHLFPAYRGLTDADKAQVDSMQSYGIKTCHIMGYMVANKGGYDAVGFTKKDLYNYFGKKMGIDIKDGDVVASLSYLRGKAGLDPMLYKCSIYEFLHNFDHTLREYRNNEHVADFKSNTSDPVLITGLDNIEKDAAKIYTSEIFKKVRKHIVKSSALIVHDQLDVEDKSMFKLSKTCNEKYRKEVVYDIADSSFHCECRLFESRGFPCSHIIFVMKVVHIDHIPSSLILKRWTKNAKIPIMGTSHGNGMDSNMIELARCGAYSAACDRFCKVAAESGACFNDVMDDILELTEKYSKLKLGGAIKTQNSEGNRHIGDPDVIRSKGAPKKKTFSMKRSRHCSNCTGANHNVRTCPKRNGVNGSQINSDEESESDATKFSNAADNIDKEMSKGDLNNIHGSVRSRLTEHGRQIDKNVKEKSNSKPKQKKTKELNVVNHPIGKQIDKGESNSNLKKAKELNEIRHPDESQAIMSQVQPNIHQMHHPMPSFANSSQRLPPTNGQTPPILQQPQQNEINHPIASQPMMSTVQPNVHQMHYPMHPFANSSQVLPPMYSINGQTPLILQQPPQNETNHLGSQPMMSQVQPNVHQMHYPMYHLANSSQVLPPIHSINGQTTPILQQPPHYFFGMPRTTNGSSCIDMLQQVMNKGR