Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86418.t1 | XP_004492094.1 | 83.3 | 48 | 8 | 0 | 1 | 48 | 46 | 93 | 8.60E-13 | 82.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86418.t1 | Q5QMM3 | 66.7 | 48 | 16 | 0 | 1 | 48 | 103 | 150 | 1.1e-11 | 70.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86418.t1 | G7KVS3 | 58.0 | 81 | 33 | 1 | 1 | 80 | 79 | 159 | 6.2e-13 | 82.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene86418.t1 | TF | HB-WOX |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86418.t1 | MTR_7g023810 | 72.840 | 81 | 21 | 1 | 1 | 80 | 79 | 159 | 2.74e-34 | 115 |
| MS.gene86418.t1 | MTR_1g115315 | 63.218 | 87 | 28 | 2 | 1 | 83 | 96 | 182 | 3.73e-28 | 102 |
| MS.gene86418.t1 | MTR_1g115315 | 63.218 | 87 | 28 | 2 | 1 | 83 | 96 | 182 | 6.18e-28 | 101 |
| MS.gene86418.t1 | MTR_3g115620 | 57.971 | 69 | 26 | 1 | 1 | 66 | 93 | 161 | 1.59e-21 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86418.t1 | AT4G35550 | 57.333 | 75 | 27 | 1 | 1 | 70 | 110 | 184 | 8.73e-18 | 75.1 |
| MS.gene86418.t1 | AT1G20700 | 57.143 | 49 | 21 | 0 | 1 | 49 | 44 | 92 | 1.14e-16 | 70.1 |
| MS.gene86418.t1 | AT1G20700 | 57.143 | 49 | 21 | 0 | 1 | 49 | 106 | 154 | 3.11e-16 | 70.1 |
| MS.gene86418.t1 | AT1G20700 | 57.143 | 49 | 21 | 0 | 1 | 49 | 106 | 154 | 3.23e-16 | 70.1 |
| MS.gene86418.t1 | AT1G20710 | 48.276 | 58 | 30 | 0 | 1 | 58 | 90 | 147 | 9.33e-15 | 66.2 |
Find 15 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGAGACCAATGTATATAAT+TGG | 0.221984 | 2.3:+948286 | MS.gene86418:CDS |
| TATAAGCTACATTCTTTAGT+TGG | 0.349191 | 2.3:-948451 | None:intergenic |
| TCCAAGCAAGGAAAAGATAA+AGG | 0.385115 | 2.3:+948227 | MS.gene86418:CDS |
| CTCAGAAATTTGTCCGTGCT+TGG | 0.426170 | 2.3:-948268 | None:intergenic |
| CAAGAGAAAGCATCGGAGTA+GGG | 0.507277 | 2.3:+948332 | MS.gene86418:CDS |
| ACAAGAGAAAGCATCGGAGT+AGG | 0.511487 | 2.3:+948331 | MS.gene86418:CDS |
| GCGAATATTTGAAGCTGAGA+CGG | 0.563300 | 2.3:+948200 | MS.gene86418:CDS |
| CTGAAACCAATTATATACAT+TGG | 0.563327 | 2.3:-948292 | None:intergenic |
| GGTTTCAGAACAGACGTGCT+AGG | 0.577434 | 2.3:+948307 | MS.gene86418:CDS |
| GTTGTTGATTTAGCCAAGCA+CGG | 0.577536 | 2.3:+948255 | MS.gene86418:CDS |
| GGTTAATGTTGAATCAGAAG+TGG | 0.597468 | 2.3:+948353 | MS.gene86418:CDS |
| TGAGACGGAGTCTCCAAGCA+AGG | 0.649464 | 2.3:+948215 | MS.gene86418:CDS |
| CTTATCATCAGAATCAACCT+CGG | 0.655992 | 2.3:-948379 | None:intergenic |
| CTAGGTACAAGAGAAAGCAT+CGG | 0.670064 | 2.3:+948325 | MS.gene86418:CDS |
| TGAATCAGAAGTGGAGACCG+AGG | 0.711591 | 2.3:+948362 | MS.gene86418:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTGAAACCAATTATATACAT+TGG | - | chr2.3:948295-948314 | None:intergenic | 25.0% |
| CTGAGACCAATGTATATAAT+TGG | + | chr2.3:948286-948305 | MS.gene86418:CDS | 30.0% | |
| CTTATCATCAGAATCAACCT+CGG | - | chr2.3:948382-948401 | None:intergenic | 35.0% | |
| GGTTAATGTTGAATCAGAAG+TGG | + | chr2.3:948353-948372 | MS.gene86418:CDS | 35.0% | |
| TCCAAGCAAGGAAAAGATAA+AGG | + | chr2.3:948227-948246 | MS.gene86418:CDS | 35.0% | |
| ! | TCCTTTATCTTTTCCTTGCT+TGG | - | chr2.3:948231-948250 | None:intergenic | 35.0% |
| ! | CTAGGTACAAGAGAAAGCAT+CGG | + | chr2.3:948325-948344 | MS.gene86418:CDS | 40.0% |
| ! | GCGAATATTTGAAGCTGAGA+CGG | + | chr2.3:948200-948219 | MS.gene86418:CDS | 40.0% |
| !! | GTTGTTGATTTAGCCAAGCA+CGG | + | chr2.3:948255-948274 | MS.gene86418:CDS | 40.0% |
| CTCAGAAATTTGTCCGTGCT+TGG | - | chr2.3:948271-948290 | None:intergenic | 45.0% | |
| !! | ACAAGAGAAAGCATCGGAGT+AGG | + | chr2.3:948331-948350 | MS.gene86418:CDS | 45.0% |
| !! | CAAGAGAAAGCATCGGAGTA+GGG | + | chr2.3:948332-948351 | MS.gene86418:CDS | 45.0% |
| !! | TGAGTTTTGTGCGTCTCAAC+TGG | + | chr2.3:948410-948429 | MS.gene86418:CDS | 45.0% |
| GGTTTCAGAACAGACGTGCT+AGG | + | chr2.3:948307-948326 | MS.gene86418:CDS | 50.0% | |
| TGAATCAGAAGTGGAGACCG+AGG | + | chr2.3:948362-948381 | MS.gene86418:CDS | 50.0% | |
| !!! | GCGTCTCAACTGGCTTTTGA+TGG | + | chr2.3:948420-948439 | MS.gene86418:CDS | 50.0% |
| TGAGACGGAGTCTCCAAGCA+AGG | + | chr2.3:948215-948234 | MS.gene86418:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 948195 | 948458 | 948195 | ID=MS.gene86418 |
| chr2.3 | mRNA | 948195 | 948458 | 948195 | ID=MS.gene86418.t1;Parent=MS.gene86418 |
| chr2.3 | exon | 948195 | 948458 | 948195 | ID=MS.gene86418.t1.exon1;Parent=MS.gene86418.t1 |
| chr2.3 | CDS | 948195 | 948458 | 948195 | ID=cds.MS.gene86418.t1;Parent=MS.gene86418.t1 |
| Gene Sequence |
| Protein sequence |
